Chemical & Biomedical Imaging最新文献

{"title":"Operando Optical Imaging Reveals Beyond-Meniscus Nanoelectrochemistry Mediated by a Nanometric Wetting Film","authors":"Louis Godeffroy,&nbsp;Hugo Chotard,&nbsp;Ivette Aguilar,&nbsp;Viacheslav Shkirskiy,&nbsp;Jean-Marc Noël,&nbsp;Jean-François Lemineur,&nbsp;Frédéric Kanoufi","doi":"10.1021/cbmi.6c00040","DOIUrl":"https://doi.org/10.1021/cbmi.6c00040","url":null,"abstract":"<p>Recent studies have highlighted therole of micrometer-scale, three-phaseinterfaces─between an electrode, an electrolyte, and a gasphase─in confining and activating chemical reactions. Here,we combine scanning electrochemical cell microscopy (SECCM) to createa localized air|water|indium tin oxide (ITO) meniscus and interferencereflection microscopy (IRM) to image nanoscale electrochemical processesoccurring in its vicinity. This approach reveals that, under the conditionsused here (20 μm diameter KCl electrolyte meniscus, ca. 40–60%relative humidity, ITO partial wetting properties, and several tensof seconds of contact time), a nanometric electrolyte film extendsbeyond the visible meniscus, over several micrometers. This expandsthe three-phase region, enabling nanocatalytic sites located outsidethe meniscus-confined electrochemical cell to react. During cathodicpolarization, IRM monitors these beyond-meniscus events through theappearance of emerging optical features associated with the formationof localized electrolyte microdroplets and dendritic metallic structures.These events are quantified at the single-entity level, particularlyin the framework of the capillary work associated with microdropletgrowth and relaxation. This analysis shows that fA-level electrochemicalactivity can be partly converted into mechanical capillary work viaelectrolyte transport through the nanometric electrolyte film. Wefinally discuss how such thin-film-mediated peripheral activity canpotentially bias meniscus-confined electrochemical measurements.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1858–1868"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810286","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Deciphering Anion-Induced Dynamic Heterogeneity of Electrochemiluminescence on Single Gold Microplates via Multi-Resolved Imaging","authors":"Zhihao Hu,&nbsp;Haihan He,&nbsp;Lisi Wen,&nbsp;Jiaxin Mao,&nbsp;Yang Wang,&nbsp;Rui Hao","doi":"10.1021/cbmi.5c00298","DOIUrl":"https://doi.org/10.1021/cbmi.5c00298","url":null,"abstract":"<p>Electrochemiluminescence (ECL) microscopy has been widelyusedfor visualization of electrochemical heterogeneity. Nevertheless,given the intricate nature of ECL reaction mechanisms, directly correlatingthe observed luminescence distribution with the material’sintrinsic catalytic activity is open to interpretation. In this work,the origin of Ru(bpy)₃²⁺/DBAE ECL heterogeneityon single Au microplates is investigated by in situ multiresolvedECL imaging/visible absorption imaging. The results demonstrate thatthe suppressed ECL activity on basal planes arises from the sluggishelectrocatalytic kinetics of the coreactant DBAE. Specifically, visibleabsorption imaging reveals a dense accumulation of DBAE oxidationproducts on the basal planes, indicating kinetic stagnation and slowdesorption that restrict the flux of radical intermediates requiredfor emission. To overcome this “adsorption-induced inhibition”,a strategy involving anion-regulated interfacial reconstruction isintroduced. The specific adsorption of sulfate anions forms a structuralscaffold within the electric double layer that physically disruptsthe dense accumulation of DBAE oxidation products. This reconstructionsupersedes the intrinsic facet-dependence of the gold surface, establishinga uniform kinetic environment that homogenizes ECL emission acrossthe entire microplate. These findings underscore the critical roleof coreactant kinetics in governing ECL heterogeneity and, furthermore,provide a mechanistic foundation for tailoring electrocatalysis viarational electrolyte design.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1785–1793"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810302","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Label-Free Imaging of Matrix Mineralization in Alginate-Encapsulated Bone Spheroids Using Coherent Raman Scattering Microscopy","authors":"Diamante Boscaro,&nbsp;Sebastian J. Kjeldgaard-Nintemann,&nbsp;Astrid Bjørkøy,&nbsp;Pawel Sikorski","doi":"10.1021/cbmi.6c00026","DOIUrl":"https://doi.org/10.1021/cbmi.6c00026","url":null,"abstract":"<p>Three-dimensional (3D) cell cultures, such as spheroids,are increasinglyused to perform advanced studies on bone matrix mineralization. However,their full characterization remains challenging. Traditional colorimetricand fluorescent assays using dyes, such as Alizarin Red S (ARS) andcalcein, are effective in monolayer cell cultures but fail to providereliable information when used in complex 3D cell constructs. In thisstudy, we investigated the application of Coherent Raman Scatteringmicroscopy for the label-free, comprehensive characterization of extracellularmatrix (ECM) mineralization in alginate-encapsulated bone spheroids.After confirming that traditional staining techniques are unreliablefor mineral detection in spheroids, Stimulated Raman Scattering (SRS)microscopy was used to detect phosphate-rich mineral deposits at aRaman shift of 960 cm^–1, while Second Harmonic Generation(SHG) microscopy was used in association with SRS to provide complementaryinformation on the deposition and organization of the collagenousmatrix. SRS was used to detect lipid-rich regions at a Raman shiftof 2857 cm^–1 to perform cell localization. SRS imagingrevealed the presence of phosphate-rich regions in the spheroids,including the core regions, which are usually challenging to characterizein intact 3D constructs. Raman spectral scans of SRS-positive regionsconfirmed the specificity of the phosphate signal. In addition, comparisonof SRS and Coherent Anti-Stokes Raman Scattering (CARS) demonstratedthe advantage of SRS in terms of reduced background compared withCARS for lipid imaging. Taken together, our results demonstrated thatSRS, in combination with SHG, provides a promising and powerful approachto performing label-free, chemically specific characterization ofintact 3D bone models.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1837–1849"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810377","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Catalyzing MRI: An Enzymatic Strategy for In Vivo Glucose Imaging","authors":"Wen Sun,&nbsp;Jong Seung Kim","doi":"10.1021/cbmi.6c00050","DOIUrl":"https://doi.org/10.1021/cbmi.6c00050","url":null,"abstract":"","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1555–1557"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810304","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"TyrRu: A Tyramide-Amplified Multimodal Ruthenium Tag for Elemental Mass Spectrometry and Immunofluorescence Imaging","authors":"Rosemary J. Bergin,&nbsp;Ioannis Kohilas,&nbsp;Thomas E. Lockwood,&nbsp;Andrew M. McDonagh,&nbsp;Mika T. Westerhausen,&nbsp;David P. Bishop","doi":"10.1021/cbmi.6c00007","DOIUrl":"https://doi.org/10.1021/cbmi.6c00007","url":null,"abstract":"<p>Tyramide signal amplification, used in immunohistochemistrytoimage low-abundance biomolecules, has seen adaptation beyond conventionalmicroscopy and is utilized in flow cytometry and electron microscopy.Here, we applied tyramide signal amplification to image biomoleculesvia elemental mass spectrometry imaging (MSI). Elemental MSI techniquessuch as laser ablation-inductively coupled plasma-mass spectrometry(LA-ICP-MS) are used to image biomolecules via metal-conjugated probes,and resolution is typically hampered by a low detection sensitivity.We report the synthesis of a bespoke ruthenium complex (<strong>TyrRu</strong>) for tyramide amplification and describe its application as a multimodalanalyte to image and quantify glial fibrillary acidic protein in mousebrain by complementary immunofluorescence and elemental MSI. Pairing <strong>TyrRu</strong> and avidin–biotin-HRP amplification produceda signal-to-noise ratio 2× higher than that obtained with a commercialmetal-chelating polymer, allowing LA-ICP-MS image resolution of 1μm. Data obtained using immunofluorescence and LA-ICP-MS showedexcellent biomolecule agreement (<em>r</em> = 0.86). <strong>TyrRu</strong> was also applied alongside primary-conjugated antibodiesin a multiplexed analysis, and the enhanced detection sensitivityfacilitated the generation of an image with submicrometer resolutionafter super-resolution reconstruction.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1805–1815"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810300","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Perspective on Scanning Electrochemical Probe Microscopy in Single-Cell Imaging","authors":"Jingjing Song,&nbsp;Yuheng Lv,&nbsp;Feixiang Fang,&nbsp;Xige Ren,&nbsp;Fei Li","doi":"10.1021/cbmi.5c00296","DOIUrl":"https://doi.org/10.1021/cbmi.5c00296","url":null,"abstract":"<p>Single-cell analysis is essentialfor probing cellularheterogeneityand understanding biological and disease mechanisms. Cellular imagingplays a key role in this field by providing critical spatiotemporaland functional information. Scanning electrochemical probe microscopy(SEPM), a kind of electrochemical imaging technique using micro/nanometer-sizedprobes to scan cells, can provide information on cellular topographyand various interfacial processes in a nondestructive and real-timemanner with high spatiotemporal resolution, therefore becoming a powerfultool for single-cell analysis. In this perspective, we systematicallyreview the advances of three SEPM techniques (including scanning electrochemicalmicroscopy (SECM), scanning ion conductance microscopy (SICM), andscanning electrochemical cell microscopy (SECCM) for single-cell analysis,including imaging cellular topography, membrane protein distribution,metabolic activity, surface charge, and mechanical property. We alsodiscuss the current challenges in enhancing spatiotemporal resolution,detection sensitivity, and imaging throughput and realizing the multiparametermonitoring capability of SEPM for single-cell studies. Last, we highlighttheir promising development directions, such as high-performance imaging,multimodal integration, functionalized probes, 4D monitoring, andintelligent data analysis, with which SEPM can support more in-depthinvestigation in cell function studies.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1558–1569"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810305","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"An Alginate Hydrogel-Based Novel Oral Contrast Agent Designed for Gastric Computed Tomography Imaging","authors":"Xiaoqiang Shang,&nbsp;Wenyu Ling,&nbsp;Ronghuan Wan,&nbsp;Hongkai Xie,&nbsp;Shiyang Zhang,&nbsp;Weiyu Shu,&nbsp;Yufeng Chen,&nbsp;Hua Zhang","doi":"10.1021/cbmi.6c00037","DOIUrl":"https://doi.org/10.1021/cbmi.6c00037","url":null,"abstract":"<p>In gastric computed tomography (CT), oral contrast agentscan enhancethe image quality, thereby improving the diagnostic sensitivity andeffectiveness for the early detection of gastrointestinal diseases.However, current oral contrast agents are mostly liquid and sufferfrom rapid gastric emptying rates and short retention times, resultingin unsatisfactory CT results. Here, we present a contrast agent thatenables calcium carbonate and sodium alginate to undergo a gelationreaction in the acidic environment of the stomach, facilitating the <em>in situ</em> formation of an iohexol-incorporated calcium alginatehydrogel-based contrast agent. Results from <em>in vivo</em> high-resolution CT scans in mice demonstrate that this hydrogel-basedcontrast agent remains stable in the stomach for 40 min, whereas liquidcontrast agents (like diluted iohexol) were significantly expelledin less than 20 min. After CT scanning, rapid dissolution and excretioncan be achieved within 30 min by oral administration of a chelatingagent, demonstrating good safety. Compared with traditional liquidcontrast agents, this hydrogel-based contrast agent exhibited strongergastric mucosa adhesion, better gastric distention, and a longer gastricretention time. Therefore, the inherent properties of hydrogel materialsfacilitate better imaging during gastric CT scans, and they hold potentialfor application in the diagnosis of gastric disorders.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1850–1857"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrabroadband Multiplex CARS Imaging Reveals Distinct Protein- and Lipid-Rich Domains in Arbuscules","authors":"Amu Sofue,&nbsp;Naoya Takeda,&nbsp;Shinsuke Shigeto","doi":"10.1021/cbmi.6c00006","DOIUrl":"https://doi.org/10.1021/cbmi.6c00006","url":null,"abstract":"<p>Arbuscular mycorrhizal (AM) fungi form mutualistic symbioseswitha wide range of terrestrial plants, in which they exchange inorganicnutrients taken up from the soil for plant-derived photosynthatessuch as sugars and lipids. This nutrient exchange occurs exclusivelyat arbuscules─highly branched hyphal structures that developin the plant root cortex. Although the molecular mechanisms underlyingarbuscule development and function have been extensively studied usingtargeted approaches based on transgenic plants and fluorescence microscopy,directly visualizing the various molecular components crucial forarbuscule function using nontargeted approaches remains a major challenge.Here, we demonstrate that ultrabroadband multiplex coherent anti-StokesRaman scattering (CARS) microspectroscopy enables two- and three-dimensionalimaging of arbuscule-containing regions in plant roots. Using geneticallytransformed <em>Lotus japonicus</em> roots colonizedby the AM fungus <em>Rhizophagus irregularis</em>, we identify Raman spectroscopic signatures of arbuscules indicativeof protein-rich domains. These domains are associated with periarbuscularmembrane-embedded transporters that are involved in AM nutrient exchange,including phosphate transporters, as well as other proteins presentin fungal and plant cells adjacent to the arbuscules. Additionally,we detect adjacent lipid-rich regions corresponding to arbuscule trunksthat mainly contain unsaturated triacylglycerols transferred fromthe host plant. Our findings highlight the potential of ultrabroadbandmultiplex CARS imaging as a label-free, in situ imaging tool for studyingarbuscules, providing deeper chemical insights into AM symbiosis.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1794–1804"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810387","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Subtle Twist on Sensor Design: NO-Induced Nitration-Triggered C–C Bond Cleavage","authors":"Ananya Dutta,&nbsp;Atul Katarkar,&nbsp;Stefan M. Huber,&nbsp;Aminur Khan,&nbsp;Mahammad Ali","doi":"10.1021/cbmi.5c00219","DOIUrl":"https://doi.org/10.1021/cbmi.5c00219","url":null,"abstract":"<p>A benzochromene-dihydropyridine-based probe (CQME) undergoescarbon–carbonbond cleavage upon exposure to NO, producing a bright fluorescentcompound (PYNO₂) and a nonfluorescent compound (PYMAA),resulting in an enhancement of fluorescence intensity by about 29times at 615 nm when excited at 470 nm along with a huge Stokes shift(∼145 nm) that reduces interference from autofluorescence,making it advantageous for <em>in vivo</em> applications.The most striking observation in this context is the C–C bondcleavage between benzochromene and dihydropyridine moieties (DIPY)for both CQME and CQET which is facilitated by NO mediated nitrationat the C2* atom. Both the probe (CQME) and the product (PYNO₂) were structurally characterized by a single-crystal X-ray diffractionmethod. Extension of this study to a simple chromene-DIPY based probe(SALDPY) gives dimethyl 4-(2H-chromen-3-yl)-2,6-dimethylpyridine-3,5-dicarboxylate(SALPY) as the major product (∼50%) with a very small amount(∼3%) of C-nitrated product (SALNO₂) and the originof these different reactivities was investigated computationally.Again, the cell permeability, notable selectivity, sensitivity (LOD∼ 42 nM), and pH independent fluorescence properties of CQMEmake it an outstanding candidate for monitoring endogenous and exogenousNO in the nanomolar range.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1678–1683"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810139","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A pH/ATP Dual-Sensitive Nanoprobe-Based Bioluminescence Imaging for Intraoperative Human Multicancer Detection","authors":"Xiaofeng He,&nbsp;Jiahui Wang,&nbsp;Junwei Li,&nbsp;Yangsi Zheng,&nbsp;Wenqi Shi,&nbsp;Jiatian Song,&nbsp;Jiayu Li,&nbsp;Zhiyong Wu,&nbsp;Menglong Zhao,&nbsp;Tiantian Zhai,&nbsp;Siqi Qiu","doi":"10.1021/cbmi.5c00256","DOIUrl":"https://doi.org/10.1021/cbmi.5c00256","url":null,"abstract":"<p>Current intraoperative cancer diagnosispredominantly relies onfrozen section histopathology, which is time-consuming and prone tosampling bias. Development of techniques for intraoperative cancerdiagnosis is urgently needed. Notably, malignant tumors manifest distinctivemicroenvironmental signatures characterized by acidic pH gradientsand elevated ATP concentrations, which are important biomarkers fortumor identification. Based on these signatures, a pH/ATP dual-sensitivebioluminescent nanoprobe, <span>d</span>-luciferase-liposome-PEOz (DLPNPs),was engineered using thin-film hydration. The nanoprobe displayeda spherical morphology (∼140 nm in diameter), low pH-dependentactivation (pH &lt;6.5), and good biocompatibility <em>in vitro</em>. <em>Ex vivo</em> bioluminescence imaging of freshly resectedspecimens from five murine tumor models (breast, liver, colon, lung,and tongue) and five human cancer types (breast, colorectal, endometrial,lung, and gastric) demonstrated significantly elevated tumor signalscompared to normal tissues. The median tumor-to-background ratios(TBRs) were 21.49 (IQR, 8.86–105.7) in mice and 47.89 (IQR,5.27–238.9) in humans. The nanoprobe achieved 100% sensitivityand 100% specificity in cancer diagnosis, with an area under the curve(AUC) of 1.00 across all cancer types evaluated in both mouse andhuman tissues. Microscopic analysis further confirmed precise colocalizationbetween bioluminescent signals and malignant regions. Notably, theentire workflow, from nanoprobe application to image acquisition,requires less than 10 min. These findings indicate that DLPNPs enablerapid and accurate intraoperative tumor diagnosis by leveraging tumormicroenvironmental signatures. The multicancer applicability, highTBR, and streamlined workflow indicate that DLPNPs are worthy of furtherevaluation for clinical translation in future studies with largersample sizes and more diverse cancer cohorts.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"4 8","pages":"1706–1717"},"PeriodicalIF":6.7,"publicationDate":"2026-08-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148810309","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}