{"title":"Precision Imaging of Biothiols in Live Cells and Treatment Evaluation during the Development of Liver Injury via a Near-Infrared Fluorescent Probe.","authors":"Yinshuang Yang, Xiaolan Liu, Deyang Xi, Yibin Zhang, Xiucai Gao, Kai Xu, Haiying Liu, Mingxi Fang","doi":"10.1021/cbmi.4c00048","DOIUrl":null,"url":null,"abstract":"<p><p>In this study, a biothiol-sensitive near-infrared (NIR) fluorescent sensor, BDP-CYS, based on a coumarin-hemicyanine skeleton, was designed and developed based on thiol-halogen S<sub>N</sub>Ar nucleophilic substitution. BDP-CYS was successfully implemented to ratiometrically monitor endogenous and exogenous Cys, Hcy, and GSH in living cells as well as to distinguish between normal and cancer cells. Furthermore, the probe was utilized to detect changes of biothiols in drug-induced hepatotoxicity and evaluate the treatment effectiveness of diabetes-associated liver injury in vivo. The advantages of BDP-CYS's Cys, Hcy, and GSH include practical sensitivity, high selectivity, rapidity of reaction, and stability across a range of pH and temperature conditions, thus introducing a new tool to better understand the roles of biothiols in oxidative stress.</p>","PeriodicalId":53181,"journal":{"name":"Chemical & Biomedical Imaging","volume":"3 3","pages":"169-179"},"PeriodicalIF":0.0000,"publicationDate":"2024-08-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11937986/pdf/","citationCount":"0","resultStr":null,"platform":"Semanticscholar","paperid":null,"PeriodicalName":"Chemical & Biomedical Imaging","FirstCategoryId":"1085","ListUrlMain":"https://doi.org/10.1021/cbmi.4c00048","RegionNum":0,"RegionCategory":null,"ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":null,"EPubDate":"2025/3/24 0:00:00","PubModel":"eCollection","JCR":"","JCRName":"","Score":null,"Total":0}
引用次数: 0
Abstract
In this study, a biothiol-sensitive near-infrared (NIR) fluorescent sensor, BDP-CYS, based on a coumarin-hemicyanine skeleton, was designed and developed based on thiol-halogen SNAr nucleophilic substitution. BDP-CYS was successfully implemented to ratiometrically monitor endogenous and exogenous Cys, Hcy, and GSH in living cells as well as to distinguish between normal and cancer cells. Furthermore, the probe was utilized to detect changes of biothiols in drug-induced hepatotoxicity and evaluate the treatment effectiveness of diabetes-associated liver injury in vivo. The advantages of BDP-CYS's Cys, Hcy, and GSH include practical sensitivity, high selectivity, rapidity of reaction, and stability across a range of pH and temperature conditions, thus introducing a new tool to better understand the roles of biothiols in oxidative stress.
期刊介绍：
Chemical & Biomedical Imaging is a peer-reviewed open access journal devoted to the publication of cutting-edge research papers on all aspects of chemical and biomedical imaging. This interdisciplinary field sits at the intersection of chemistry physics biology materials engineering and medicine. The journal aims to bring together researchers from across these disciplines to address cutting-edge challenges of fundamental research and applications.Topics of particular interest include but are not limited to:Imaging of processes and reactionsImaging of nanoscale microscale and mesoscale materialsImaging of biological interactions and interfacesSingle-molecule and cellular imagingWhole-organ and whole-body imagingMolecular imaging probes and contrast agentsBioluminescence chemiluminescence and electrochemiluminescence imagingNanophotonics and imagingChemical tools for new imaging modalitiesChemical and imaging techniques in diagnosis and therapyImaging-guided drug deliveryAI and machine learning assisted imaging
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