JBRA assisted reproduction最新文献

{"title":"First Brazilian birth after Preimplantation Genetic Testing for Severe Methylmalonic Acidemia: preventing sudden infant death.","authors":"Rafael Berteli Fontes, Cezar Antonio Fontes, Rita de Cássia Sousa-Polezzi, Rita de Cássia Fuga Berteli Fontes, Taccyanna Mikulski Ali, Ana Cerveró","doi":"10.5935/1518-0557.20260031","DOIUrl":"https://doi.org/10.5935/1518-0557.20260031","url":null,"abstract":"<p><p>Methylmalonic acidemia (MMA) is a rare autosomal recessive metabolic disorder caused by pathogenic variants in the MMUT gene, often associated with severe neonatal manifestations and sudden unexpected death in infancy (SUDI). Preimplantation Genetic Testing for Monogenic Diseases (PGT-M) offers a valuable reproductive option for at-risk couples seeking to prevent transmission of such conditions. This represents the first Brazilian case of a healthy birth following combined PGT-M and PGT-A for MMA known. After the neonatal loss of their first child due to mut0-type MMA, a couple underwent genetic testing, which identified compound heterozygosity in MMUT: a maternal deletion (c.1196_1197del) and a paternal duplication (c.213_216dup). Haplotype construction used parental and proband DNA. Two IVF cycles yielded 13 blastocysts; of these, one embryo was diagnosed as being both euploid and unaffected, and two were euploid and heterozygous carriers. One of those embryos was selected for transfer, resulting in an uneventful pregnancy and the live birth of a healthy female infant. This single case highlights the clinical utility of PGT-M to prevent the recurrence of a severe inborn errors of metabolism and underscores the importance of early genetic counseling in couples with prior adverse neonatal outcomes.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Endometrioma may differentially affect eicosanoids in the follicular fluid and serum of infertile women with endometriosis.","authors":"Larissa de Oliveira Koopman, Mayra Beraldo Andozia, Carlos Arterio Sorgi, Lucia Helena Faccioli, Michele Gomes da Broi, Paula Andrea Navarro","doi":"10.5935/1518-0557.20260033","DOIUrl":"https://doi.org/10.5935/1518-0557.20260033","url":null,"abstract":"<p><strong>Objective: </strong>The etiopathogenesis of endometriosis-related infertility remains under investigation, including in women with endometriomas. Oocyte quality is believed to play an important role in the impairment of natural fertility in these patients, with systemic inflammation and oxidative stress acting as mediators of oocyte damage. Eicosanoids are bioactive lipids produced in response to inflammatory or oxidative conditions and are involved in various ovarian processes; their dysregulation may impair oocyte competence. This study aimed to compare the serum and follicular fluid eicosanoid profiles of women with and without endometriosis, including women with and without endometrioma.</p><p><strong>Methods: </strong>In this prospective cohort study, blood and follicular fluid samples were collected on the day of oocyte retrieval from 18 infertile women with endometriosis (8 with endometrioma and 10 without endometrioma) and 20 patients with tubal and/or male-factor infertility. The eicosanoid profiles of the samples were analyzed by liquid chromatography-tandem mass spectrometry (LC-MS/MS).</p><p><strong>Results: </strong>No differences were found in serum eicosanoids between the endometriosis and control groups. After subdividing the endometriosis group into women with and without endometrioma, we observed lower serum levels of 5-hydroxyeicosatetraenoic acid (5-HETE) in those with endometrioma (38.47pg/mL vs. 120.45pg/mL; p=0.01). Increased follicular levels of eicosapentaenoic acid (EPA) were found in women with endometriosis (4038.52pg/mL; p=0.03) and in those with endometrioma (5811.50pg/mL; p=0.005) compared with controls (1159.83 pg/mL).</p><p><strong>Conclusion: </strong>The findings suggest that pelvic disease promotes a systemic pro-inflammatory alteration, whereas endometrioma induces a local anti-inflammatory response. This may represent a mechanism to prevent oocyte damage, highlighting different mechanisms underlying endometriosis-related infertility according to lesion type. Further studies should confirm these findings using appropriate sample sizes and methodology.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-07-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440683","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Differential Expression of ZNF83, ACY-1, and SMC5 in Cumulus Cells: A Potential Biomarker for Oocyte Fertilization Competence.","authors":"Azam Govahi, Ehsan Raoufi, Mahsaneh Vatankhah, Elham Hosseini, Hadis Gholipour, Fatemehsadat Amjadi","doi":"10.5935/1518-0557.20260022","DOIUrl":"10.5935/1518-0557.20260022","url":null,"abstract":"<p><strong>Objective: </strong>Since cumulus cells (CCs) play an undeniable role in oocyte maturation by producing and transferring important molecules to the oocyte, examining these cells can provide a broad view of the factors affecting oocyte competence. Therefore, the aim of this study was to investigate the expression of genes and proteins ZNF83, ACY-1, andSMC5 in CCs of unfertilized oocytes and compare it with fertilized oocytes to gain new insights into the relationship between CCs function and oocyte competence.</p><p><strong>Methods: </strong>Eighteen healthy female oocyte donors were included in this study. After obtaining the cumulus-oocyte complex and isolating the CCs, oocytes were injected and the embryos were followed and morphologically graded on day 3. Then the expression of genes and proteins ZNF83, ACY-1, andSMC5 in CCs of non-fertilized oocytes and CCs from fertilized oocytes was investigated and compared.</p><p><strong>Results: </strong>The expression of ZNF83, ACY-1, and SMC5 at gene and protein levels was reduced in the non-fertilized group compared to the fertilized group (p<0.05).</p><p><strong>Conclusions: </strong>The results highlight CCs' critical role in supporting oocyte fertilization. These findings show the way for novel biomarkers and therapeutic strategies to improve IVF outcomes. More functional studies are needed to support the hypothesis of this study and explore the clinical applications of these markers in ART.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"437-442"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544261/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of three embryo culture media: A randomized clinical trial with sibling oocytes.","authors":"Bruna Campos Galgaro, Lisiane Knob de Souza, Luíza da Silva Rodrigues, João Sabino Lahorgue da Cunha-Filho","doi":"10.5935/1518-0557.20260025","DOIUrl":"10.5935/1518-0557.20260025","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the influence of three culture media on the good quality cleavage embryo and fertilization rates.</p><p><strong>Methods: </strong>A three-arm, prospective, randomized, controlled clinical trial included patients undergoing IVF treatments at a single center from May to August 2023. Sibling oocytes were randomized in three culture media (A, B and C). Fertilization and day three good quality embryo rates were evaluated.</p><p><strong>Results: </strong>Three hundred sixty-four retrieved oocytes from forty patients were randomized right after ovarian aspiration. From those, 309 metaphase II oocytes (mean per patient 7.72±2.87) were fertilized and cultured. Overall fertilization rate was 81.6%, with no statistical difference among media: 85.8% (73-100) vs. 77.2% (63-100) vs. 82.4% (67-100) for A, B and C, respectively (p=0.290). Although the percentage of D3 good quality embryos in each media was not statistically significant [A: 52.3% (0-76), B: 35.9% (0-67), C: 44.8% (0-100) (p=0.137)], the number of patients with at least one good quality embryo on D3 was statistically different [28 (70.0%) vs. 18 (45.0%) for A and B, respectively (p=0.020) OR 1.56; 95% CI (1.05-2.32, p=0.020)]. In a multivariable analysis, culture media and age have significantly affected D3 embryo quality OR 1.44; 95% CI (1.22-2.35, p=0.016).</p><p><strong>Conclusions: </strong>Although no difference was observed in fertilization and cleavage-stage embryo quality rate among brands, one culture medium had a significantly lower number of patients with good-quality cleavage embryos. Further trials with pregnancy outcomes are warranted to confirm clinical implications of these findings.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"443-449"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544258/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440638","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Impact of chronic endometritis treatment in patients with reproductive failure.","authors":"Sara Vázquez Ramos, Alejandro Olloqui Escalona, Jorge García Ibáñez, Laura de la Fuente Bitaine, Carmen Guillén Gámez, Concepción Pérez Sagaseta, Cristina González Macho, María Concepción Carrera Roig","doi":"10.5935/1518-0557.20260049","DOIUrl":"10.5935/1518-0557.20260049","url":null,"abstract":"<p><strong>Objective: </strong>To assess whether the diagnosis and antibiotic treatment of chronic endometritis improve reproductive outcomes in patients with recurrent pregnancy loss or recurrent implantation failure.</p><p><strong>Methods: </strong>This retrospective study included 67 women with recurrent pregnancy loss or recurrent implantation failure. Chronic endometritis was diagnosed in 30 patients (44.8%) based on hysteroscopic findings, CD138-positive histology, or a positive endometrial culture. All patients diagnosed with chronic endometritis received first-line antibiotic therapy, most commonly doxycycline 200 mg/day for 14 days.</p><p><strong>Results: </strong>Following antibiotic therapy, 17 patients (56.7%) achieved a clinical pregnancy and 12 (40%) achieved a live birth. Clinical pregnancy and live birth rates were similar in women with recurrent pregnancy loss (60% and 40%, respectively) and those with recurrent implantation failure (55% and 40%, respectively), with no statistically significant differences between patients with resolved and persistent chronic endometritis (p>0.05).</p><p><strong>Conclusion: </strong>In this study, antibiotic treatment for chronic endometritis was not associated with a statistically significant improvement in reproductive outcomes. The heterogeneity of diagnostic and therapeutic approaches highlights the need for standardized criteria and well-designed prospective studies to clarify its clinical relevance.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"569-573"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544562/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effect of depression, anxiety, and cognitive flexibility on pregnancy outcomes in women with unexplained infertility.","authors":"Kübra Dilşad Sönmez Özgür, Beyazıt Garip, Kazım Emre Karaşahin","doi":"10.5935/1518-0557.20260032","DOIUrl":"10.5935/1518-0557.20260032","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to investigate the interaction between depression, anxiety, and cognitive flexibility in women with unexplained infertility and to evaluate the impact of these psychological factors on pregnancy outcomes following assisted reproductive treatments (ART). The study also sought to determine whether cognitive flexibility could serve as a potential neuropsychological determinant influencing reproductive success.</p><p><strong>Methods: </strong>A total of 120 women aged 20-40 years were enrolled: 66 with unexplained infertility and 54 healthy controls with spontaneous conception. The infertility group underwent assisted reproductive treatments (ART) and was divided into pregnant (n=35) and nonpregnant (n=31) subgroups. Depression, anxiety, and cognitive flexibility were assessed using Beck Depression Inventory (BDI), Beck Anxiety Inventory (BAI), and the State-Trait Anxiety Inventory (STAI-I/II) and Wisconsin Card Sorting Test (WCST).</p><p><strong>Results: </strong>Depression scores significantly decreased in women who conceived after ART (p=0.012), whereas both depression and anxiety levels increased in those who did not achieve pregnancy (p<0.001). No significant changes were observed in the control group (p≥0.050). Women who conceived demonstrated significantly higher WCST category completion scores compared to those who did not conceive (p=0.046), while no group difference was found in perseverative error counts.</p><p><strong>Conclusion: </strong>Cognitive flexibility may influence treatment success in unexplained infertility. Higher WCST category completion in women who conceived suggests that supporting psychological resilience and adaptive coping may contribute to improved emotional well-being and reproductive outcomes.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"469-474"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544255/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440760","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of chlamydia infection history on tubal patency: A pilot study.","authors":"Julià Alvarez-Puig, Nuria Muñiz-López, Laura Melé, Montserrat Cubo-Abert, Alba Noguera-Solà, Nuria Esteve-Vila, José Moreno-Sepulveda, Ana Calvo-Redol, Marta Simó-Gonzalez, Juan J Espinós","doi":"10.5935/1518-0557.20260036","DOIUrl":"10.5935/1518-0557.20260036","url":null,"abstract":"<p><strong>Objectives: </strong>Chlamydia infection is a known risk factor for tubal occlusion and subsequent infertility. A significant proportion of Chlamydia infections are asymptomatic, which can delay diagnosis and allow silent progression of tubal damage. Therefore, identifying a history of Chlamydia infection-even in the absence of symptoms-may help recognize women at risk of tubal involvement. In such cases, it would be justified to perform tubal patency testing to assess potential tubal and pelvic sequelae. Our purpose was to evaluate the relationship of Chlamydia IgG serology with tubal patency as determined by Hysterosonosalpingo-Foam-Sonography (HyFoSy). Furthermore, the study examines whether this information could guide decision-making in the initial clinical evaluation of infertile patients.</p><p><strong>Methods: </strong>A retrospective pilot study was performed including all patients referred for primary infertility to the Reproductive Medicine Unit of the Gynecology Service of the Hospital Arnau de Vilanova in Lleida between 2022 and 2024. Chlamydia infection history was assessed through serological IgG testing, and tubal patency was evaluated using HyFoSy.</p><p><strong>Results: </strong>Seventy-nine patients were included in the study. A Chlamydia IgG seropositivity rate of 13.9% was observed, while tubal occlusion-assessed through HyFoSy-was detected in 25.3% of cases. Patients younger than 33 years with a positive Chlamydia IgG testing showed a higher rate of tubal occlusion compared to patients with negative serology (OR 8.00, 95% CI [1.08, 59.16]). No differences were observed in older patients.</p><p><strong>Conclusions: </strong>Chlamydia infection may be associated with a higher risk of tubal occlusion in younger patients, especially those under 33 years.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"498-505"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544263/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440689","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Endometrial Thickness Predict Chemical Pregnancy in Frozen Embryo Transfer? Analysis of 522 artificial cycles performed by a single physician.","authors":"Lorena Cristina Santos, Mario Silva Approbato, Alexandre Vieira Santos Moraes, Jane Porfírio Rocha, Gustavo Cardoso Borges, Amanda A Araujo, Julia Coelho Masiero, Eduardo Camelo de Castro","doi":"10.5935/1518-0557.20260038","DOIUrl":"10.5935/1518-0557.20260038","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate whether endometrial thickness (EMT) is associated with pregnancy rates in frozen embryo transfer (FET) cycles.</p><p><strong>Methods: </strong>This retrospective study analyzed medical records of patients who underwent FET between January 2021 and December 2024. All procedures were conducted by the same physician, using standardized endometrial preparation, with blastocyst-stage transfers. EMT was measured by transvaginal ultrasound on the day of transfer, and pregnancy was confirmed by β-hCG > 25 mIU/ml approximately two weeks later. Statistical analysis included Mann-Whitney U test, linear regression, ROC curve analysis, and chi-square tests, with significance defined as p≤0.05.</p><p><strong>Results: </strong>A total of 522 FET cycles were performed, after exclusions, such as untreated uterine infertility factors and cryopreserved cleavage stage embryos, 478 patients were included with a chemical pregnancy rate of 70.29%. Participants' median age was 34.9 years (range 23-49). EMT ranged from 3.6 to 20mm (mean 8.82mm). ROC analysis identified 8.3 mm as the optimal cutoff (AUC=0.55; sensitivity 0.542; specificity 0.518). No significant differences were found in pregnancy rates between different EMT intervals (≤8.3mm vs. >8.3 mm, p=0.4944; <7 mm vs. ≥7mm, p=0.1036). Regression analysis showed no significant association between EMT and pregnancy (b=-1.205; p=0.3182; 95% CI: -3.914 to 1.504).</p><p><strong>Conclusion: </strong>In our study, EMT did not significantly predict pregnancy outcomes in FET cycles, indicating that the cancellation of embryo transfers should not be canceled solely on the basis of endometrial thickness.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"519-523"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544269/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440693","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Role of Artificial Intelligence in Addressing Menopausal Mental Health Challenges.","authors":"Rowaida Sadat, Koray Gorkem Sacinti, Andrea Panattoni, Stefano Luisi","doi":"10.5935/1518-0557.20260019","DOIUrl":"10.5935/1518-0557.20260019","url":null,"abstract":"<p><p>Menopause marks a crucial transition in a woman's life and is often accompanied by physical and psychological changes that can adversely affect mental health. Depression, anxiety, cognitive changes, and sleep disturbances are common during the menopausal transition, yet they are frequently underdiagnosed and undertreated, particularly in lowand middle-income countries. Emerging technologies, especially artificial intelligence (AI), offer new opportunities to narrow this care gap. Although AI has shown considerable promise in identifying menopause-related physical health conditions (e.g., osteoporosis and endometrial cancer), its use for mental health during this life stage remains limited. We discuss the potential of AI-driven tools-including machine learning algorithms, digital therapeutics, symptom trackers, and large language models-to improve the detection, monitoring, and personalized management of menopause-associated mental health disorders. By integrating genetic, clinical, lifestyle, and wearable data, AI systems may help predict risk, identify symptom patterns, and support tailored interventions. These approaches could enable scalable, accessible, and cost-effective mental healthcare, reduce stigma, and address service gaps. Harnessing AI in this area offers a significant opportunity to improve quality of life for millions of women worldwide.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"658-659"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544265/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro effect of fresh and frozen seminal plasma on the proliferation and viability of periodontal ligament fibroblasts.","authors":"Sara Valentina Oquendo Cano, Manuela González Muñoz, Mónica Alejandra Mahecha Peña, Jenniffer Puerta Suárez","doi":"10.5935/1518-0557.20260048","DOIUrl":"10.5935/1518-0557.20260048","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effects of fresh and frozen seminal plasma, at three different concentrations, on the migration and viability of periodontal ligament fibroblasts.</p><p><strong>Methods: </strong>Semen samples from three healthy volunteers were processed to obtain seminal plasma, filtered (0.22 µm), and diluted to 2.5% and 5% (v/v) in DMEM. Aliquots were tested either fresh or after storage at -20°C for 8 days. Cell migration was evaluated by measuring wound closure in a wound-healing assay using photographs analyzed with ImageJ. Cell proliferation was assessed in triplicate at 6, 12, and 24 hours using the commercial CellTiter 96® AQueous Non-Radioactive Cell Proliferation Assay kit.</p><p><strong>Results: </strong>Across donors, frozen seminal plasma showed a higher mean percentage of wound closure at 48 hours than fresh seminal plasma; however, differences versus controls were not consistently significant, and viability tended to decrease after exposure to seminal plasma, particularly after freezing.</p><p><strong>Conclusion: </strong>Fresh and frozen seminal plasma did not promote cell migration or proliferation at the concentrations tested. The apparent trend toward greater migration with frozen samples requires confirmation in adequately powered studies and should be interpreted cautiously.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"562-568"},"PeriodicalIF":1.9,"publicationDate":"2026-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13544557/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440728","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}