JBRA assisted reproduction最新文献

{"title":"Introducing the Blastocyst Fragmentation Indicator (BFI): A Novel Time-Lapse Metric for Enhanced Aneuploidy Risk Stratification in Non-Invasive Embryo Assessment.","authors":"Hamilton de Martin, Eduardo Gomes Sá, Andrea Mesquita Lima, Ellayne Cavalcanti Queiroz, Gleicyane Sousa Dos Santos Alam, Tulius Augustus Ferreira de Freitas, Gabriel Acácio de Moura, Eduardo de Paula Miranda, Sebastião Evangelista Torquato","doi":"10.5935/1518-0557.20250171","DOIUrl":"10.5935/1518-0557.20250171","url":null,"abstract":"<p><strong>Objective: </strong>To propose an improved blastocyst grading system by integrating dynamic time-lapse parameters with conventional morphological criteria, aiming to enhance embryo selection during assisted reproduction.</p><p><strong>Methods: </strong>This retrospective cohort study evaluated 1,182 embryos derived from 433 cycles of intracytoplasmic sperm injection. Time-lapse parameters included morula compaction status, blastocyst collapse frequency, and fragmentation of the inner cell mass and trophectoderm. These findings were analyzed in conjunction with standard morphological grading and embryo scoring systems based on implantation data. Embryo outcomes were stratified by chromosomal status determined through preimplantation genetic testing for aneuploidy.</p><p><strong>Results: </strong>Fully compacted morulae were associated with higher morphological grades; however, the proportion of chromosomally normal embryos did not differ significantly when compared to those derived from partially compacted morulae, suggesting that static morphology alone may not accurately reflect developmental potential. Although most blastocyst collapse events were minor, they correlated with increased fragmentation and reduced embryo quality. Fragmentation of the inner cell mass and/or the trophectoderm was associated with significantly lower rates of chromosomal normalcy. Logistic regression analysis confirmed fragmentation as the strongest predictor of aneuploidy, outperforming both conventional grading systems.</p><p><strong>Conclusions: </strong>This integrated evaluation model, combining static morphology with dynamic developmental events, offers a more comprehensive and accurate approach to assessing embryo viability. By identifying embryos with higher risk of chromosomal abnormalities, this strategy may enhance embryo selection, improve reproductive outcomes, and contribute to more individualized care in assisted reproduction.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"132-141"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059644/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Is there an increased risk of preeclampsia using donor sperm, depending on sexual condition?","authors":"Begoña Prieto, Natalia Sanz, María Díaz-Nuñez, Lucía Lainz, Silvia Pérez-Fernández, Olatz Molina, Ainara Bengoetxea, Maitane Gantxegi, Roberto Matorras","doi":"10.5935/1518-0557.20250155","DOIUrl":"10.5935/1518-0557.20250155","url":null,"abstract":"<p><strong>Objective: </strong>An increased risk of preeclampsia has been described when performing donor intrauterine insemination. One of the possible causes is immunological tolerance to semen. Both lesbian and single women, who have theoretically lower exposure to seminal fluid than heterosexual women requesting artificial insemination with donor, sperm should have a higher incidence of preeclampsia, although this fact has not been analyzed previously.</p><p><strong>Methods: </strong>The population under study consisted of 439 gestations <24 weeks, achieved with artificial insemination with donor sperm, performed in two different populations: heterosexual couples and SLTG (single women, lesbian women, women with transgender partner). Preeclampsia rates and other perinatal outcomes were compared.</p><p><strong>Results: </strong>No significant differences were found in the development of preeclampsia in the SLTG group 6.03% (12/199) vs. heterosexual patients 5.83% (14/240), p=0.93. In the SLTG group, compared with heterosexual group, age (36 vs. 35, p=0.002) and BMI (24.8 vs. 23.6, p=0.002) were somewhat higher. After adjusting for BMI, age and multiplicity of gestation, there were no significant differences in the risk of preeclampsia between SLTG group and heterosexual women. As expected, in women with preeclampsia, gestational ages and newborn weights (quantitative markers of preeclampsia severity) were significantly lower than in women without preeclampsia.</p><p><strong>Conclusions: </strong>Our study suggests that neither sexual orientation nor previous sperm exposure carries a higher risk of preeclampsia, especially after correction for age and weight. Artificial insemination with donor sperm performed on single women, lesbians or women with transgender partners is a safe, simple and efficient technique, just as in heterosexual couples.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"47-55"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145544792","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Effect of Vitex Agnus - Castus Plant on Some Markers of Oxidative Stress, Lipid Profile and Insulin Resistance in Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind Controlled Clinical Trial Study.","authors":"Aniseh Hatami, Fateme Seidi, Ali Khosrowbeygi, Azam Moslemi, Farideh Jalali-Mashayekhi","doi":"10.5935/1518-0557.20250165","DOIUrl":"10.5935/1518-0557.20250165","url":null,"abstract":"<p><strong>Objective: </strong>This randomized controlled trial investigated the efficacy of standardized Vitex agnus-castus extract in managing features of PCOS. The primary aim was to assess changes in oxidative stress markers; secondary outcomes included lipid profile, insulin resistance, and clinical signs such as hirsutism and menstrual frequency.</p><p><strong>Methods: </strong>Sixty women with PCOS were randomly assigned to a Vitex group (5.8 mg daily, standardized to 0.42-0.82 mg Aucubin) or placebo for 12 weeks. Dietary habits and physical activity and physical activity were maintained throughout the study. Serum total antioxidant capacity, glutathione peroxidase, reduced glutathione, and other biochemical and clinical parameters were assessed preand post-intervention. Between-group differences were analyzed using independent t-tests and ANCOVA.</p><p><strong>Results: </strong>Compared to placebo, Vitex significantly increased total antioxidant capacity (effect size = 13.01), glutathione peroxidase (3.35), reduced glutathione (3.88), total thiol (3.34), and HDL (5.74) (all p<0.05). It decreased total oxidant status (-6.49), oxidative stress index (-9.30), malondialdehyde (-5.29), fasting blood sugar (-5.10), HOMA-IR (-0.31), LDL (-2.85), ALT (-3.51), and mFG score (-5.38). Menstrual frequency improved (3.51), and left ovarian volume reduced (-0.80).</p><p><strong>Conclusions: </strong>Vitex agnus-castus improved oxidative stress markers and insulin resistance and favorably modulated clinical manifestations of PCOS. These findings suggest a clinically meaningful benefit and support further investigation into Vitex as an adjunctive therapy.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"70-78"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055162/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Exposure to oral contraceptives alters human endometrial stem cells culture media secretome.","authors":"Raquel Cellin Rochetti, Fernanda Bertuccez Cordeiro, Larissa Berioni Rodrigues da Silveira, Lívia do Vale Teixeira da Costa, Renato Fraietta, Kayla Jane Perkel, Fernando Prado Ferreira, Edson Guimaraes Lo Turco","doi":"10.5935/1518-0557.20250194","DOIUrl":"10.5935/1518-0557.20250194","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the effect of oral contraceptives on the secretome of endometrial mesenchymal stem cells (EnMSC) and their potential impact on endometrial plasticity.</p><p><strong>Methods: </strong>The EnMSC were collected from menstrual shedding of five volunteers and cultured for three passages. Cells were characterized by flow cytometry and culture media was collected at the end of each passage for further secretome analysis. Quantitative analysis included detection of aminoacids, biogenic amines, acylcarnitines, lysophosphatidylcholines, phosphatidylcholines, sphingomyelins, and hexose. Data was analyzed by partial least square analysis. Potential biomarkers were analyzed by receiver operating characteristic (ROC) curve.</p><p><strong>Results: </strong>From 186 metabolites quantified in the culture media of OC and non-OC groups, 15 metabolites were identified as of high discrimination between groups by the PLS-DA. The ROC curve showed that 4 out of 15 metabolites presented more than 80% of sensitivity. These metabolites are Alanine, Phosphatidylcholine (PC) aa C30:0, Glycine and PC aa C32:2, whose concentrations were higher in the OC group than in non-OC group. The Students' T-Test analysis confirmed that Alanine was significantly higher in the OC group (p=0.00176).</p><p><strong>Conclusions: </strong>The use of OCs could affect endometrial plasticity and influence the reproductive success. This study provides a preliminary insight into the EnMSCs response to OCs based on specific metabolite signatures, which may contribute to the comprehension of mechanism associated with EnMSC and OCs.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"31-38"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055182/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314518","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Beneficial Effects of Hesperidin and Exercise on the Histology and Biochemical Parameters of Surgically Induced Endometriosis in a Rabbit Model.","authors":"Idowu Sunday Oyeleye, Barakat Olamide Ishola, Busuyi Akinola, Abiola Hannah Oduntan, Victor Okoliko Ukwenya, Ganiyu Oboh","doi":"10.5935/1518-0557.20250183","DOIUrl":"10.5935/1518-0557.20250183","url":null,"abstract":"<p><strong>Objective: </strong>Endometriosis is a gynecological disorder marked by the formation of endometrial tissue (gland and stroma) outside the uterine cavity. Macrophages, erythrocytes, and apoptotic endometrial tissue transplant into the peritoneal cavity, free radicals, and oxidative stress play a vital role in endometriosis. This study focused on unraveling the combinatorial effect of treadmill exercise with hesperidin (HESP, a citrus flavonoid) on histoarchitecture and biochemical [reactive oxygen species (ROS), nitric oxide (NO), total thiol (T-SH), glutathione (GSH), TBARS levels, catalase, glutathione peroxidase (GPx), superoxide dismutase (SOD), glutathione-s-transferase (GST), and monoamine oxidase activities] molecules in endometrial tissue of female rabbits.</p><p><strong>Methods: </strong>The rabbits underwent surgery by resecting one uterine horn, isolating the endometrium, and fixing the tissue segment to the pelvic peritoneum, and they were distributed into 5 groups (n = 6). Group 1: normal control (NC) Group 2: untreated endometriotic rabbits (ENDO); Group 3: HESP-treated endometriotic rabbits (eNDO + HESP); Group 4: exercised endometriotic rabbits (ENDO + EXER); Group 5: exercised endometriotic rabbits administered with HESP (ENDO + HESP + EXER).</p><p><strong>Results: </strong>The results revealed that antioxidant enzyme activities and non-antioxidant molecules were reduced in untreated endometriotic rabbits compared to NC. However, there was a significant increase in antioxidant status in exercised endometriotic rabbits treated with hesperidin.</p><p><strong>Conclusions: </strong>This finding revealed that combining physical exercise with the consumption of hesperidin-rich fruits can be explored to alleviate oxidative stress, a major risk factor in endometriosis.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"170-179"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055166/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314524","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"JBRA Assisted Reproduction: three decades of science, innovation, and leadership in Latin America.","authors":"Paulo Franco Taitson, Maria do Carmo Borges de Souza, Roberto de Azevedo Antunes, João Batista Alcantara Oliveira","doi":"10.5935/1518-0557.20260013","DOIUrl":"10.5935/1518-0557.20260013","url":null,"abstract":"","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":"30 1","pages":"1-2"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13060014/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147597620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of human papillomavirus on semen parameters and male infertility: a single-center study.","authors":"Stefan Matik, Ana-Marija Bosilkovska, Simona Bardakoska-Stefanovska, Ivana Matik, Sotir Ropi, Aleksandar Jovanovski, Hristijan Trpchevski, Marija Petrusevska","doi":"10.5935/1518-0557.20250186","DOIUrl":"10.5935/1518-0557.20250186","url":null,"abstract":"<p><strong>Objective: </strong>To detect the presence of seminal HPV and examine its association with semen quality in men from infertile and fertile couples.</p><p><strong>Methods: </strong>This single-center case-control study involved 50 men from infertile couples and 10 proven-fertility men as controls. Semen samples collected were analyzed using computer-aided semen analysis and tested for HPV detection and genotyping with real-time PCR.</p><p><strong>Results: </strong>HPV DNA was detected in the semen of 9 men from infertile couples (18.0%) and 1 proven-fertility control (10.0%). Among infertile couples, HPV positivity was linked to significantly altered semen parameters: increased progressive motility (p=0.0399), higher presence of sperm with excess residual cytoplasm (p=0.05), and a lower percentage of normal acrosomes (p=0.05). Kinematic measurements, including straight-line velocity (VSL, p=0.0235), average path velocity (VAP, p=0.0434), linearity (LIN, p=0.05), wobble (WOB, p=0.05), and beat cross frequency (BCF, p=0.0239), were significantly higher in HPV-positive infertile men. Compared to fertile controls, HPV-positive men from the infertile group showed significantly lower sperm concentration (p=0.0493), total motile sperm count (p=0.0291), and mucus penetration ability (p=0.0088), along with a reduced percentage of morphologically normal sperm (p=0.05). Conversely, they had higher rates of sperm with excess residual cytoplasm (p=0.05), tail deformities (p=0.05), and neck and midpiece deformities (p=0.0499).</p><p><strong>Conclusions: </strong>Seminal HPV is associated with impaired sperm parameters in infertile men. This highlights HPV's role as a potential contributing factor in male infertility, warranting further investigations.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"99-106"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055435/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812212","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Piperine protects ovarian follicles and stromal cells against doxorubicin-induced adverse effects in mouse ovaries.","authors":"Moisés Bruno Marinho Rocha, Ernando Igo Teixeira de Assis, Venância Antônia Nunes Azevedo, Miguel Fernandes de Lima Neto, Anderson Weiny Barbalho Silva, Alana Nogueira Godinho, Jordânia Marques de Oliveira Freire, Emanoel da Silva Félix, Regislane Pinto Ribeiro, Geovany Amorim Gomes, José Roberto Viana Silva","doi":"10.5935/1518-0557.20250187","DOIUrl":"10.5935/1518-0557.20250187","url":null,"abstract":"<p><strong>Objective: </strong>This study investigates the effects of Piperine (PIP) on doxorubicin (DOX)-induced changes in mouse ovarian follicles, stromal cells, collagen fibers, and mRNA expression of nuclear factor erythroid 2-related factor (NRF2), superoxide dismutase (SOD), and catalase (CAT).</p><p><strong>Methods: </strong>The mice were randomly divided into seven groups. In the first three groups, they received saline (1), both DOX and N-acetylcysteine (2), or DOX only (3). In groups 4 and 5, mice were treated with DOX in combination with 0.1 or 10.0mg/kg PIP. In groups 6 and 7, mice received 0.1 or 10.0mg/kg PIP alone. After 10 days, ovaries were collected and used to evaluate follicular morphology and growth, collagen fibers, stromal cells, and mRNA for NRF2, SOD, and CAT.</p><p><strong>Results: </strong>Mice treated with DOX showed reduced percentage of normal follicles, but the combination of DOX with PIP or NAC prevented this effect, maintaining follicle integrity similar to untreated animals. Ovaries of mice treated with PIP alone had similar percentage of normal follicles compared to control group. Additionally, the association of DOX and PIP preserved collagen levels similar to control, while PIP or NAC alone did not influence collagen distribution. Ovaries of mice treated with both DOX and NAC showed a reduction in stromal cells, but those treated with both DOX and PIP maintained the levels of collagens similar to control.</p><p><strong>Conclusions: </strong>The DOX and PIP preserved the integrity of follicles and collagen fibers in mouse ovaries, which opens a new possibility to protect primordial follicles during chemotherapy.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"79-88"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055168/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of fertilization media on in vitro embryonic development in red-rumped agouti () using a heterologous system with guinea pig oocytes.","authors":"Lhara R M de Oliveira, Leonardo V C de Aquino, Luana G P Bezerra, Moacir F de Oliveira, Alexandre R Silva, Alexsandra F Pereira","doi":"10.5935/1518-0557.20250180","DOIUrl":"10.5935/1518-0557.20250180","url":null,"abstract":"<p><strong>Objective: </strong>This study aimed to evaluate the effects of different heterologous in vitro fertilization (he-IVF) media on embryonic development in red-rumped agoutis (Dasyprocta leporina) using guinea pig oocytes as a model. Considering the species' ecological and biotechnological relevance, optimizing IVF conditions is essential to improve assisted reproductive technologies for conservation and research.</p><p><strong>Methods: </strong>Epididymal spermatozoa from four red-rumped agoutis were selected and capacitated before co-incubation with in vitro matured guinea pig oocytes. Three IVF media were tested: human tubal fluid (HTF), Toyoda-Yokoyama-Hoshi (TYH), and Tyrode's albumin lactate pyruvate (TALP). After six hours of fertilization, presumptive zygotes were cultured and evaluated on Days 2 and 5 for cleavage, morphology, assessments of oocyte-sperm interaction, and number of cells. In addition, reactive oxygen species (ROS) levels, and mitochondrial membrane potential assessments were performed to analyze oxidative stress during early development.</p><p><strong>Results: </strong>Among the tested media, TALP significantly improved cleavage rates (p<0.05) and resulted in a higher proportion of embryos with eight or more cells on both D2 and D5 of culture (p<0.05). Embryos derived from TALP also showed reduced oxidative stress, evidenced by lower ROS levels and decreased mitochondrial membrane potential compared to the other groups (p<0.05).</p><p><strong>Conclusions: </strong>TALP proved to be the most effective medium for supporting he-IVF using red-rumped agouti sperm, enhancing both developmental kinetics and cellular quality. These results contribute to the refinement of IVF protocols and represent an important step toward developing homologous embryo production systems in this neotropical species.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"191-202"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059645/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812255","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Zingerone Mitigates Testicular Dysfunction Induced by Cisplatin.","authors":"Elham Younesi, Layasadat Khorsandi, Amirhesam Keshavarz Zarjani, Abbas Heidari-Moghadam, Mohammad Javad Khodayar, Yousef Asadi-Fard","doi":"10.5935/1518-0557.20250181","DOIUrl":"10.5935/1518-0557.20250181","url":null,"abstract":"<p><strong>Objective: </strong>Cisplatin is one of the most widely used antitumor drugs globally, particularly in treating various solid tumors. The reproductive system is impacted by cisplatin toxic effects. This study aims to understand how Zingerone affects spermatogenesis defects in mice.</p><p><strong>Methods: </strong>In the present experimental laboratory study, the 48 male NMRI mice (6 to 8 weeks of age, 25 to 30g weight) were treated with Cisplatin (7 mg/kg) for 5 days and zingerone for 30 days at concentrations of 10, 20, and 40 mg/kg before cisplatin administration. After the treatment period, the testicles were dissected immediately following sacrifice. Morphometric parameters, serum testosterone concentration, histology, Bax/Bcl-2 ratio, and testis weight have been assessed. To determine levels of oxidative stress, malondialdehyde contents and antioxidant levels were evaluated.</p><p><strong>Results: </strong>Cisplatin-induced structural damages enhanced the Bax/Bcl-2 ratio, and reduced testosterone levels and testis weight. Cisplatin caused oxidative stress by enhancing malondialdehyde contents in the mouse testicles. Zingerone dose-dependently reduced the Bax/Bcl-2 ratio and reversed the histological changes, testosterone levels, and antioxidant capacity.</p><p><strong>Conclusions: </strong>According to the results of the present study, Pretreatment with zingerone can improve testosterone production by preventing apoptosis and oxidative stress in the testicles of mice that have undergone cisplatin intoxication.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"203-212"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13059646/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}