JBRA assisted reproduction最新文献

{"title":"Contraceptive-induced impairment: a rodent model study of levonorgestrel and DMPA.","authors":"F A Akinpelu, O L Apanisile, B M Oguntimilehin, E N Akang","doi":"10.5935/1518-0557.20250041","DOIUrl":"https://doi.org/10.5935/1518-0557.20250041","url":null,"abstract":"<p><strong>Objective: </strong>Hormonal contraceptives are widely used to alter the hypothalamic-pituitary-gonadal (HPG) axis, inhibiting ovulation, and altering reproductive hormone levels. Understanding their effects on estrous cycle regulation and fecundity in animal models is essential for evaluating their contraceptive mechanisms and long-term reproductive impact. This study aims to investigate the effects of Levonorgestrel and Depot-Medroxyprogesterone Acetate (DMPA) on estrous cycle phases, hormonal disruptions, and fecundity in rodent models, providing insights into their contraceptive efficacy and potential reproductive consequences.</p><p><strong>Methods: </strong>Fifteen adults female Wistar rats were divided into three groups: Control (water only), Levonorgestrel-treated (0.7mL oral administration), and DMPA-treated (0.02mL intramuscular injection). Vaginal cytology was used to track estrous cycle phases before, during, and after treatment. Mating success and fecundity were assessed by pairing treated females with males and recording pregnancy rates and litter sizes.</p><p><strong>Results: </strong>Both Levonorgestrel and DMPA significantly disrupted estrous cycle regularity. The estrus phase duration was notably shortened in treated groups, while the diestrus phase was prolonged, especially in the DMPA group (p<0.0001). Mating success was significantly reduced, with only 40% of DMPA-treated and 60% of Levonorgestrel-treated females mating successfully, compared to 100% in the control group. Litter numbers were also significantly lower in treated groups compared to controls.</p><p><strong>Conclusions: </strong>These findings suggest that prolonged use of Levonorgestrel or DMPA may significantly alter the oestrous cycle, potentially impairing fertility and delaying reproductive recovery. Further studies are needed to explore the long-term consequences of contraceptive-induced cycle disruptions.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254390","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Monosodium glutamate is associated with dose-dependent reproductive toxicity and sperm dysfunction in male wistar rats.","authors":"Olaleke Abraham Fasasi, Babatunde Oluwaseun Ibitoye, Adesua Emmanuel Ogunmokunwa, Adebanji Modupe Akingbade, Adebukola Olubosede Omolayo","doi":"10.5935/1518-0557.20250052","DOIUrl":"https://doi.org/10.5935/1518-0557.20250052","url":null,"abstract":"<p><strong>Objective: </strong>Monosodium glutamate (E621) is widely used as a flavor enhancer and is added to many processed foods, often concealed by the label E621. Controversies exist regarding its safety, as most studies use high doses that are challenging to translate to human relevance. This study investigated the effects of chronic low-dose MSG administration, mimicking average daily human usage, on male Wistar rats' reproductive function.</p><p><strong>Methods: </strong>Thirty rats were divided into five groups (n=6): Group A (control, 1 ml distilled water) and Groups B, C, D, and E, receiving MSG doses of 30 mg/kg, 100 mg/kg, 300 mg/kg, and 1000 mg/kg body weight, respectively, for 30 days. Parameters measured included body weight, testicular weight, and semen analysis (sperm concentration, morphology, and motility).</p><p><strong>Results: </strong>Body weight increased significantly in MSG-treated groups, particularly at higher doses (240±20 g for 300 mg/kg and 260±25 g for 1000 mg/kg). Gonadosomatic Index (GSI) rose dose-dependently, while testicular weight declined in higher-dose groups (1.2±0.1g at 1000 mg/kg). Sperm concentration reduced from 80±5 million/ml (control) to 30±2 million/ml (1000 mg/kg), and normal sperm morphology dropped from 76% to 58%. Defects in sperm head, neck, and tail increased with dose, and motility showed a marked decline, with fast progressive sperm decreasing to 39% (1000 mg/kg) and non-motile sperm rising to 51%.</p><p><strong>Conclusions: </strong>These findings suggest that chronic low-dose MSG exposure negatively impacts male reproductive health, emphasizing the need to assess dietary MSG's long-term risks.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of the biomechanical characteristics of human ovarian tissue after vitrification versus slow freezing.","authors":"Marta Méndez, Carolina Herranz-Diez, Santiago González, Janisse Ferreri, Josep Maria Calafell, Jordi Otero, Ramon Farre, Salvadora Civico, Francesc Fabregues","doi":"10.5935/1518-0557.20250045","DOIUrl":"https://doi.org/10.5935/1518-0557.20250045","url":null,"abstract":"<p><strong>Objective: </strong>Numerous studies have compared ovarian tissue cryopreservation methods, including slow freezing/rapid thawing (SF/RT) and vitrification/warming (V/W), focusing on morpho-functional status, follicle density, stromal cell integrity, and gene expression during in vitro culture. However, results remain inconclusive. This study aims to evaluate the effects of SF/RT and V/W on the ovarian cortex's biomechanical properties.</p><p><strong>Methods: </strong>Human ovarian biopsies were taken from five women between 32 and 45 years undergoing laparoscopic surgery for tubal sterilization. For each patient, one small sample of fresh tissue was used as control, and the remaining tissue were vitrified or frozen by slow freezing method. The tissue stiffness of the cryopreserved samples at the micrometer scale was measured by Atomic Force Microscopy after thawing and warming, depending of the cryopreservation method.</p><p><strong>Results: </strong>The median stiffness of the ovarian cortex was 3670.00 Pa (Pascal) (IQR 2146.4) in the control group. After cryopreservation, the median stiffness slightly decreased to 1305.90 Pa (IQR 503.51) with SF/RT and to 2284.50 Pa (IQR 3314.40) with V/W. General linear model analysis revealed no significant effect of cryopreservation method on the ovarian cortex stiffness (F=2.750, p=0.071). No significant differences were observed based on the intra-sample zone studied by AFM. However, a significant inter-patient effect on tissue stiffness was identified (F=3.958, p=0.006).</p><p><strong>Conclusions: </strong>The study findings suggest that ovarian tissue freezing methods do not have a relevant impact on functional aspects of the extracellular matrix (ECM), suggesting that given the logistical advantages of vitrification, this technique should be prioritized.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254472","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association between anti-Mullerian hormone and antithyroid antibodies detectability and positivity in women assisted at a fertility clinic.","authors":"Yuri Ian Lima de Oliveira, Arnaldo Couto, Paulo Gallo de Sá, Lenora Maria Camarate Silveira Martins Leão, Ana Beatriz Winter Tavares","doi":"10.5935/1518-0557.20250032","DOIUrl":"https://doi.org/10.5935/1518-0557.20250032","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate the association between antithyroid antibodies detectability and positivity and ovarian reserve. Additionally, we aimed to evaluate the correlation between TSH levels and parameters of ovarian reserve [anti-Mullerian (AMH) levels and antral follicle count (AFC)].</p><p><strong>Methods: </strong>Subjects were women seeking assisted reproductive therapy and oocyte cryopreservation. AMH, AFC and antithyroid antibodies were determined. In a subgroup of patients, antithyroid antibodies were stratified into categories for the association analyses. The reference values were as follows: antithyroid peroxidase antibody (TPOAb): undetectable (<9 IU/mL), low detectable (9-21 IU/mL), high detectable (22-34 IU/mL), and positive (> 34 IU/mL); antithyroglobulin antibody (TgAb): undetectable (< 10 IU/mL), low detectable (10-60 IU/mL), high detectable (61-115 IU/ mL), and positive (>115 IU/mL). AMH levels < 1.1 ng/mL and/or AFC < 7 follicles were considered to indicate low ovarian reserve.</p><p><strong>Results: </strong>A total of 453 patients were enrolled. 24.4% had at least one positive antithyroid antibody. Low detectable values for TPOAb and TgAb were found in 42.3% and 66.9%, respectively. TPOAb and TgAb levels were not statistically different between women with AMH < or ≥ 1.1 ng/mL; there was also no statistically significant difference between antithyroid antibodies levels and AFC < or ≥ 7 follicles. AMH levels did not show significant association with detectability nor positivity of the antithyroid antibodies. No statistically significant correlation was observed between AMH and TSH nor AFC and TSH.</p><p><strong>Conclusions: </strong>Although the detectability of TPOAb has been associated with different outcomes, TPOAb and/or TgAb were not associated with ovarian reserve in our study.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Efficiency of mild ovarian stimulation with letrozole co-treatment in expected low ovarian responders.","authors":"Alfredo Cortés-Vazquez, Ismael Perez-Bautista, J Andreas Mejicanos-Quiñonez, Hector R Salazar-Ochoa, M Carmen Peraza-Briones, J Daniel Moreno-García, Alfredo L Cortés-Algara","doi":"10.5935/1518-0557.20250161","DOIUrl":"https://doi.org/10.5935/1518-0557.20250161","url":null,"abstract":"<p><strong>Objective: </strong>Ovarian stimulation (OS) is a critical step in assisted reproductive techniques (ART); it has been routinely adopted since it substantially increases the whole process efficiency. Our study aims to compare the efficiency of mild OS with letrozole co-treatment vs. conventional OS in expected low ovarian responders.</p><p><strong>Methods: </strong>A single-centre, observational, comparative, non-experimental and retrospective study using our database at the Reproductive Endocrinology Department at Centro Médico Nacional 20 de Noviembre in Mexico City was performed.</p><p><strong>Results: </strong>We compared the total rFSH dosage per oocyte, we noticed a statistically significant difference between groups, Group A 388.9 vs. Group B 667.2IU per retrieved oocyte (p<0.0002, 105 to 450 IU CI 95%), Comparing total rFSH dosage per follicle, we noticed a statistically significant difference among Group A 397.5 vs. Group B 590.2IU per follicle (p<0.014, 39.2 to 346.2 IU, CI 95%).</p><p><strong>Conclusions: </strong>In expected poor ovarian responders, mild ovarian stimulation with letrozole co-treatment is much more efficient than conventional stimulation.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254474","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of infertility-associated autoantibodies and sexually transmitted infections on implantation success rates after ICSI.","authors":"Natalia Bergamo Saraiva Bacarov, Tabea Hammes, Jens Miguel Warnecke, Rafaela Hidalgo, Ana Carolina Bernardo, Claudia Fideles, Letícia Dargenio Garcia, Caio Parente Barbosa, Michel Moraes Soane, Denise Maria Christofolini","doi":"10.5935/1518-0557.20250157","DOIUrl":"https://doi.org/10.5935/1518-0557.20250157","url":null,"abstract":"<p><strong>Objective: </strong>Preimplantation genetic testing improves rates of assisted reproductive technologies success by selecting euploid embryos, reducing implantation failures and miscarriages. However, some factors in women may also impact outcomes, though systematic studies on autoantibodies and implantation success are lacking. In this study, we determined the presence of specific autoantibodies related to infertility and specific antibodies to sexually transmitted infections pathogens among women undergoing intracytoplasmic sperm injection including preimplantation genetic testing and investigated their effect on implantation success.</p><p><strong>Methods: </strong>Plasma samples from 86 women undergoing in vitro fertilization procedures by intracytoplasmic sperm injection and preimplantation genetic testing were tested for the presence of specific autoantibodies against the ovaries, placenta, uterus, and sperm as well as specific antibodies against Mycoplasma hominis, Ureaplasma urealyticum, Treponema pallidum, Chlamydia Trachomatis, Herpes simplex virus 1 and Herpes simplex virus 2. The prevalences of these antibodies were compared between women with successful versus unsuccessful implantation after embryo transfer.</p><p><strong>Results: </strong>The prevalence of anti-Ureaplasma urealyticum antibodies was significantly higher (Fisher's exact test, p=0.028) in women with unsuccessful implantation after intracytoplasmic sperm injection procedures (62.0%), compared to women with successful implantation (36.1%).</p><p><strong>Conclusions: </strong>The results of this study indicate that a past infection with Ureaplasma urealyticum might lead to an increased risk for implantation failure after intracytoplasmic sperm injection procedures. Thus, the detection of IgG antibodies against Ureaplasma urealyticum could potentially be used in the future to identify women with an increased risk for implantation failure. However, our findings need to be confirmed in larger cohorts.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-10-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145254450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Does Sleep Quality Affect IVF Outcomes?","authors":"Lara S Pamfilio, Elaine C F de Oliveira, Frederico T R Sousa, Maira Casalechi, Fernando M Reis","doi":"10.5935/1518-0557.20250048","DOIUrl":"https://doi.org/10.5935/1518-0557.20250048","url":null,"abstract":"<p><strong>Objective: </strong>This narrative review aims to summarize the current understanding of the relationship between sleep quality and outcomes in in vitro fertilization (IVF) treatments. An intricate connection links sleep and reproductive health. Sleep affects hormonal regulation and reproductive processes by several physiological mechanisms, underscoring the importance of considering sleep as a modifiable factor in fertility treatments. Evidence suggests that sleep habits and quality can affect IVF outcomes like clinical pregnancy and live birth rates. However, the heterogeneity in study designs and methodologies poses challenges in drawing definitive conclusions. Further research should employ standardized and objective measures of sleep quality to deepen our understanding and improve clinical guidance for couples undergoing IVF. The potential for behavioral interventions to enhance sleep quality offers a promising opportunity for improving fertility outcomes, warranting more focused investigations in this area.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133369","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Repeated gonadotropin stimulation modulates the expression of specific proteins in mouse oviduct.","authors":"Gianna Rossi, Sabrina Colafarina, Osvaldo Zarivi, Anna Maria Giuseppina Poma, Sandra Cecconi","doi":"10.5935/1518-0557.20250050","DOIUrl":"https://doi.org/10.5935/1518-0557.20250050","url":null,"abstract":"<p><strong>Objective: </strong>Superovulation protocol modifies the oviductal site-specific expression of some proteins regulating cell cycle and oxidative stress response.</p><p><strong>Methods: </strong>Swiss CD1 female mice (n=24) were sorted into 2 groups: one was used as control (Ctr, n=10), the other consisted of mice undergoing 8 Rounds (8R) of repeated gonadotropin stimulation (n=14). After their removal, oviducts were cut into two portions: one including Infundibulum and Ampulla (If-Am), and the rest including Isthmus (Is). Both portions were separately used to assess the expression levels of oviductal proteins regulating cell cycle and oxidative stress response. Statistical analysis employed t-test with significance at p<0.05.</p><p><strong>Results: </strong>In Ctr mice, superoxide dismutases 1 and 2 were significantly more expressed in the If-Am, while phospho-p53, glutathione peroxidase 1 and estrogen receptor beta mainly in the Is. Conversely, catalase, cleaved-caspase 3, estrogen alpha and progesterone receptors were similarly distributed across the oviduct. After 8R, glutathione peroxidase 1 and superoxide dismutase 1 increased in both segments, superoxide dismutase 2 and cleaved-caspase 3 increased mainly in If-Am, while catalase and phosphorylated p53 mainly in Is. Estrogen alpha/beta and progesterone receptors levels remained unchanged.</p><p><strong>Conclusions: </strong>Altogether, these results demonstrated that in the mouse oviduct many proteins were expressed in a site-specific manner and that repeated gonadotropin stimulation could modulate their expression levels. These data suggest that different localization of proteins between Infundibulum-Ampulla and Isthmus regions is fundamental for creating a suitable microenvironment for embryo development.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Bisphenol A and human fertility: a systematic review.","authors":"Carolina Paz Mohamad Isa, Gabriela Barella Schmidt, Renata Guerreiro de Jesus, Joselaine Sturmer, Júlia Prauchner de Castilhos, Laura Randon Chapochnicoff, Victória Campos Dornelles, Marta Ribeiro Hentschke, Alvaro Petracco, Mariangela Badalotti","doi":"10.5935/1518-0557.20250029","DOIUrl":"https://doi.org/10.5935/1518-0557.20250029","url":null,"abstract":"<p><p>Considering the significant exposure to the synthetic compound bisphenol A (BPA), present in a wide range of materials in our daily lives, this article discusses a possible correlation between this substance and human fertility through a bibliographic review. In the context of growing evidence that BPA impacts the fertility of women and men of reproductive age, the reviewed articles suggest that exposure to this agent may affect ovarian reserve parameters in women. In pregnant women, it may cause fetal malformations. BPA has also been linked to an increase in spontaneous abortions and premature births. Additionally, it can cause hormonal disruptions, affect folliculogenesis, and worsen ovarian response in assisted reproduction, as well as lead to lower estradiol concentrations, reduced fertilization rates, and higher implantation failure. In men of reproductive age, BPA may decrease sperm production, potentially contributing to testicular dysgenesis syndrome and cryptorchidism. However, further studies are still required to better understand the diverse and complex mechanisms through which BPA affects key reproductive functions.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133368","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comment on \"The effect of COVID-19 vaccination on serum levels of anti-Mullerian hormone in women of reproductive age\".","authors":"Hinpetch Daungsupawong, Viroj Wiwanitkit","doi":"10.5935/1518-0557.20250054","DOIUrl":"https://doi.org/10.5935/1518-0557.20250054","url":null,"abstract":"","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2025-09-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145133405","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}