JBRA assisted reproduction最新文献

Influence of seminal cryopreservation on embryo kinetics. Image analysis and clinical outcomes. 精子冷冻保存对胚胎动力学的影响。图像分析和临床结果。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-03-17 DOI: 10.5935/1518-0557.20260005

M C Lorenzo Rodríguez, M Martínez Morales, I Pérez Cano, P Hernández Vargas, B Gadea Navarro, M Roldán Ramírez, B Amorocho Llanos, A Doménech Vidal, M A Valera Cerdá, M Muñoz Cantero, M Meseguer Escrivá

{"title":"Influence of seminal cryopreservation on embryo kinetics. Image analysis and clinical outcomes.","authors":"M C Lorenzo Rodríguez, M Martínez Morales, I Pérez Cano, P Hernández Vargas, B Gadea Navarro, M Roldán Ramírez, B Amorocho Llanos, A Doménech Vidal, M A Valera Cerdá, M Muñoz Cantero, M Meseguer Escrivá","doi":"10.5935/1518-0557.20260005","DOIUrl":"https://doi.org/10.5935/1518-0557.20260005","url":null,"abstract":"Objective: This study aims to compare the effect of using fresh ejaculated sperm versus cryopreserved sperm on the morphology and kinetics of embryo development and on clinical reproductive outcomes.Methods: Retrospective, observational, single-centre study conducted at IVIRMA Alicante (June 2013-December 2020) that included 469 oocyte donation cycles with frozen, fresh and mixed eggs in which ICSI, IVF or IVF-ICSI with fresh ejaculated and frozen ejaculated partner sperm was performed. For each group, the morphokinetic variables t2, t3, t4, t5, t6, t7, t8, tM, tSB, tB, tEB were studied in addition to the clinical pregnancy rate, implantation rate, ongoing pregnancy rate, miscarriage rate, and live birth rate, per transfer and per embryo transferred. Statistical analysis was performed using the IBM SPSS® software. Values of p≤0.05 were considered statistically significant.Results: Longer development times were observed in the fresh semen group, with significantly longer times for t2, t3, t4, t5, tB, tEB. Clinical results per transfer showed a trend, although not significant, towards lower rates in the cryopreserved semen group compared to the fresh semen group. However, the miscarriage rate was higher for the cryopreserved semen group, although this difference was not statistically significant.Conclusions: Our results indicate that embryos from fresh semen have a slower developmental rate for the morphokinetic parameters studied. Since no significant differences in clinical reproductive outcomes were found, it is concluded that sperm freezing is a valuable technique that can continue to be applied. However, further studies are needed to confirm these results.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The scientific gap in reproductive immunology and the financial burden on patients: a critical review. 生殖免疫学的科学差距和患者的经济负担：一个重要的回顾。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-03-17 DOI: 10.5935/1518-0557.20250191

Pedro Augusto Araujo Monteleone

引用次数: 0

Effectiveness of BMMSC and Dienogest on IL-1β, IL-8 Expression and Lesion Area in Endometriosis Mice. BMMSC和Dienogest对子宫内膜异位症小鼠IL-1β、IL-8表达及病变面积的影响。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-03-17 DOI: 10.5935/1518-0557.20260001

Rista Silvana, Yusuf Effendi, Rizani Amran, Widjiati Widjiati

{"title":"Effectiveness of BMMSC and Dienogest on IL-1β, IL-8 Expression and Lesion Area in Endometriosis Mice.","authors":"Rista Silvana, Yusuf Effendi, Rizani Amran, Widjiati Widjiati","doi":"10.5935/1518-0557.20260001","DOIUrl":"https://doi.org/10.5935/1518-0557.20260001","url":null,"abstract":"Objective: Endometriosis is a chronic estrogen-dependent inflammatory disease that impairs fertility and quality of life. Dienogest, the current standard therapy, provides symptom relief but does not correct immune imbalance or prevent recurrence. Bone marrow-derived mesenchymal stem cells (BMMSCs), with strong immunomodulatory potential, may represent a disease-modifying alternative. This study evaluated the effects of BMMSCs, Dienogest, and their combination on IL-1β and IL-8 expression and lesion size in a murine endometriosis model.Methods: The experiment was conducted between August and December 2024 using 32 female mice, randomly assigned into four groups: untreated endometriosis controls (K+), BMMSC monotherapy (P1), BMMSC combined with Dienogest (P2), and Dienogest monotherapy (P3). Mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the models received mesenchymal stem cells. Sample collection was on day 29. Cytokine expression (IL-1β and IL-8) was assessed immunohistochemically, while lesion dimensions were analyzed using ImageJ v1.54 (NIH, USA) software. Immunofluorescence confirmed the BMMSC phenotype (CD73+, CD90+, CD105+, CD45-). Statistical analysis used one-way ANOVA and Kruskal-Wallis with Bonferroni correction.Results: BMMSC monotherapy resulted in the most pronounced suppression of IL-1β and IL-8 expression, alongside a significant reduction in lesion size (p<0.001). All treatment groups differed significantly from the control group.Conclusions: BMMSC monotherapy in endometriosis mice models markedly reduced IL-1β and IL-8 expression, decreased lesion size, and demonstrated more robust disease-modifying activity compared to Dienogest or combination therapy.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Egg freezing in transgender men. 变性男性的卵子冷冻。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-03-17 DOI: 10.5935/1518-0557.20260009

Caiane Gabrielle Souza de Paula, Paula Bruno Monteiro, Raissa de Vasconcelos Cavalcanti

{"title":"Egg freezing in transgender men.","authors":"Caiane Gabrielle Souza de Paula, Paula Bruno Monteiro, Raissa de Vasconcelos Cavalcanti","doi":"10.5935/1518-0557.20260009","DOIUrl":"https://doi.org/10.5935/1518-0557.20260009","url":null,"abstract":"Transgender men are people who were biologically assigned female at birth but identify as men. It is important to consider the impacts of testosterone and reassignment surgery on fertility. Hormone therapy can compromise ovarian function, and surgery can result in the irreversible loss of reproductive capacity. Fertility preservation has emerged as a viable option for those who wish to have biological children. This study aimed to evaluate the effectiveness of this method in trans men, analyze the ovarian response to hormonal stimulation, and investigate the impact of the duration of therapy on the quality and quantity of oocytes. PubMed, SciELO, and LILACS databases were consulted, with articles from 2015 to 2025 in English, Portuguese, and Spanish, following the PRISMA guide. The data was organized in Microsoft Excel tables. Case, cohort, cross-sectional, prospective, and retrospective studies were included, excluding articles out of scope, unavailable, used animals, and reviews. Initially, 404 articles were identified, with four duplicates excluded. After screening, 25 articles remained for full reading, of which 13 were analyzed. The duration of hormone therapy varied between 2 and 12 years among the patients in the studies analyzed, and only one patient did not have sufficient data due to the absence of embryo transfer. The studies showed promising results for egg cryopreservation, even with prolonged use of testosterone, but there are still no definitive conclusions about the long-term effect on fertility. This review contributes to the understanding of fertility in trans men and their reproductive alternatives.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477911","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Comparative Efficacy of Microfluidics and Density Gradient Centrifugation for Sperm Preparation in IVF: A Randomized Controlled Trial. 微流体和密度梯度离心在体外受精精子制备中的比较效果：一项随机对照试验。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250190

Do Thuy Huong, Do Thi Minh Tam, Nguyen Thanh Hoa, Ho Nguyet Minh, Nguyen Manh Ha, Ho Sy Hung

{"title":"Comparative Efficacy of Microfluidics and Density Gradient Centrifugation for Sperm Preparation in IVF: A Randomized Controlled Trial.","authors":"Do Thuy Huong, Do Thi Minh Tam, Nguyen Thanh Hoa, Ho Nguyet Minh, Nguyen Manh Ha, Ho Sy Hung","doi":"10.5935/1518-0557.20250190","DOIUrl":"https://doi.org/10.5935/1518-0557.20250190","url":null,"abstract":"Objective: To compare the effectiveness of microfluidic sperm sorting (MC) and traditional density gradient centrifugation (DGC) in reducing sperm DNA fragmentation index (DFI) and evaluate their impact on clinical outcomes in IVF cycles.Methods: In this randomized controlled trial, 119 couples undergoing IVF were allocated to either the MC or DGC group. Sperm DNA fragmentation was assessed before and after preparation. Primary outcomes included live birth rate and DFI reduction. Secondary outcomes were sperm quality parameters, fertilization rate, embryo development, and pregnancy outcomes. Subgroup analysis was conducted based on initial DFI levels (<15% vs. ≥15%).Results: The MC group demonstrated significantly greater and more consistent DFI reduction, particularly in samples with high baseline DFI (≥15%), compared to the DGC group. Although the number of oocytes retrieved was higher in the MC group, no significant differences were observed between groups in fertilization rate, Day-2 embryo quality, clinical pregnancy, ongoing pregnancy, or live birth rates. Subgroup analysis also showed no significant differences in outcomes based on baseline DFI levels.Conclusions: Microfluidics is an effective method for reducing sperm DNA fragmentation, particularly in samples with high DFI. However, this improvement does not necessarily guarantee better clinical outcomes in IVF. Baseline sperm DNA fragmentation index (DFI) may still act as an independent prognostic factor influencing IVF success, regardless of the sperm preparation method used.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In Vitro Fertilization and Placenta Accreta (spectrum): A Systematic Review. 体外受精和胎盘增生（谱）：系统综述。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250192

Ana Carolina Wickert Theisen, Nathália Fritsch Camargo, Talita Colombo

{"title":"In Vitro Fertilization and Placenta Accreta (spectrum): A Systematic Review.","authors":"Ana Carolina Wickert Theisen, Nathália Fritsch Camargo, Talita Colombo","doi":"10.5935/1518-0557.20250192","DOIUrl":"https://doi.org/10.5935/1518-0557.20250192","url":null,"abstract":"In vitro fertilization (IVF) has transformed infertility treatment, providing options for individuals struggling to conceive naturally, with significant success rates, particularly in women under 35. However, IVF pregnancies are associated with potential obstetric complications, notably placenta accreta spectrum (PAS), which necessitates early diagnosis and a multidisciplinary approach to safeguard maternal-fetal health. This systematic review involved a comprehensive search of databases such as PubMed, Cochrane Library, BVS, Embase, Web of Science, and Scopus using specific terms related to IVF and PAS. Original studies comparing spontaneous pregnancies to IVF pregnancies were included, while case reports and non-peer-reviewed articles were excluded. Data extraction was standardized, and study quality was assessed using the Newcastle-Ottawa tool. The analysis covered thirteen articles involving 252,295 assisted reproductive technology pregnancies and over 16 million spontaneous pregnancies. A key finding is the increased risk of PAS in IVF pregnancies, with some studies indicating that frozen embryo transfer (FET) poses a higher risk than fresh embryo transfer. Additional complications include hypertension, preeclampsia, and intrauterine growth restriction (IUGR). Interestingly, some research suggests a lower Intensive Care Unit admission rate for women with placenta accreta after IVF compared to those with spontaneous conception. This indicates that while assisted reproductive technology is an independent risk factor for PAS, it presents a unique risk profile. The review emphasizes the need for further research into maternal outcomes related to PAS after assisted reproductive techniques (ART) and highlights the importance of specialized follow-up and rigorous prenatal diagnosis to minimize complications in these pregnancies.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Global Research Trends in Immunotherapies for Recurrent Pregnancy Loss: A Bibliometric Analysis. 免疫疗法治疗复发性妊娠丢失的全球研究趋势：文献计量学分析。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250188

Marcelo Borges Cavalcante, Daniel de Sousa Sobral, Raíssa Helen de Andrade Praciano, Maria Edith Holanda Banhos, Cristiana Libardi Miranda Furtado

{"title":"Global Research Trends in Immunotherapies for Recurrent Pregnancy Loss: A Bibliometric Analysis.","authors":"Marcelo Borges Cavalcante, Daniel de Sousa Sobral, Raíssa Helen de Andrade Praciano, Maria Edith Holanda Banhos, Cristiana Libardi Miranda Furtado","doi":"10.5935/1518-0557.20250188","DOIUrl":"https://doi.org/10.5935/1518-0557.20250188","url":null,"abstract":"This bibliometric study investigates global research trends in the application of immunotherapies for managing recurrent pregnancy loss (RPL). Data were retrieved from the Web of Science (WoS) database, encompassing 1,735 publications from 1964 to 2024. The analysis demonstrates a steady increase in research activity, with an average annual growth rate of 1.14%. Leading journals in the field include The American Journal of Reproductive Immunology and Human Reproduction, while prominent contributors such as Ole Bjarne Christiansen and William H. Kutteh have significantly influenced the domain. The United States, China, and the United Kingdom were identified as the most productive countries in terms of publication volume and citation metrics. Keyword co-occurrence analysis reveals a thematic progression from early-stage experimental treatments to a more advanced focus on immunological and molecular mechanisms underlying RPL. Among various therapeutic modalities, heparin, aspirin, and intravenous immunoglobulin have been the most frequently studied. Meanwhile, novel immunotherapies are emerging as promising alternatives, although their clinical efficacy remains to be thoroughly validated. This study emphasizes the critical role of international collaboration in advancing the field and highlights persistent research gaps that warrant further investigation. Overall, the findings provide a comprehensive overview of the evolution, current status, and future directions of immunotherapy research in the context of recurrent pregnancy loss.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314529","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

#infertility education on Instagram: A Reliability Analysis in Brazil Using the DISCERN Instrument. # Instagram上的不孕教育：使用DISCERN工具在巴西进行的可靠性分析。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20260006

Márcia Mendonça Carneiro, Renata Bossi, Debora Alvarenga, Ana Carolina Xavier, Rodrigo Hurtado, Marcos Sampaio, Marisa Mendonça Carneiro

{"title":"#infertility education on Instagram: A Reliability Analysis in Brazil Using the DISCERN Instrument.","authors":"Márcia Mendonça Carneiro, Renata Bossi, Debora Alvarenga, Ana Carolina Xavier, Rodrigo Hurtado, Marcos Sampaio, Marisa Mendonça Carneiro","doi":"10.5935/1518-0557.20260006","DOIUrl":"https://doi.org/10.5935/1518-0557.20260006","url":null,"abstract":"Objective: To assess the prevalence, authorship, and reliability of educational fertility-related information shared on Brazilian Instagram, measured by the DISCERN instrument.Methods: This cross-sectional study analyzed public fertility-related educational Instagram posts published in March 2021 related to the following hashtags: #infertility, #ivf, #endometriosis, #tryingtoconceive, #maternity, #humanreproduction, #pregnancy, #invitrofertilization, #assistedreproduction, #pregnant, #difficulttogetpregnant. Educational posts were evaluated using the DISCERN tool. Authorship was categorized as either healthcare professional (HCP) or layperson (LP).Results: The majority of the 37 analyzed posts were authored by HCP (n = 33; 89.2%), with 24 of these being physicians, five fertility clinics, four allied HCP, one magazine, and three LP. Most posts (n = 15) focused on fertility treatments; other topics included information about diseases, exams, and diagnosis. The mean Discern analysis scores for each question were: (1) Are the aims clear? 4.86; (2) Does it achieve its aims? 4.75; (3) Is it relevant? 4.47; (4) Is it clear what sources of information were used to compile the publication (other than the author or producer)? 1.58; (5) Is it clear when the information used or reported in the publication was produced? 1.59; (6) Is it balanced and unbiased? 1.59; (7) Does it provide details of additional sources of support and information? 1.04; (8) Does it refer to areas of uncertainty? 1.39.Conclusions: Although physicians authored most posts that clearly stated the aim and relevance, important issues such as the source of information used and details about additional sources of support and information were not available. The posts analyzed here failed to be balanced and unbiased and did not inform about potential areas of uncertainty.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314567","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In vitro maturation 2.0: a new era for an underdog in ART. 体外成熟2.0：抗逆转录病毒治疗中的弱势群体的新时代。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250177

Marcos Iuri Roos Kulmann, Gabriella Mamede Andrade, Adriana Bos-Mikich, Norma Pagnoncelli de Oliveira, Marcelo Ferreira, Nilo Frantz

{"title":"In vitro maturation 2.0: a new era for an underdog in ART.","authors":"Marcos Iuri Roos Kulmann, Gabriella Mamede Andrade, Adriana Bos-Mikich, Norma Pagnoncelli de Oliveira, Marcelo Ferreira, Nilo Frantz","doi":"10.5935/1518-0557.20250177","DOIUrl":"https://doi.org/10.5935/1518-0557.20250177","url":null,"abstract":"In vitro maturation (IVM) of human oocytes, once a pioneering concept predating conventional IVF, has long remained an underutilized technique in assisted reproduction. Despite early promise, clinical adoption of IVM has been limited due to lower embryo developmental competence and live birth rates compared to IVF. However, recent advances in the understanding of oocyte physiology, including cumulus-oocyte communication, the regulatory roles of cAMP/cGMP signaling pathways, and endocrine modulation of meiotic resumption, have reignited interest in optimizing IVM protocols. Innovations such as biphasic Capacitation (CAPA) IVM systems and the use of ovarian somatic support cells (OSCs) derived from induced pluripotent stem cells (iPSCs) aim to replicate the dynamic follicular environment more accurately and enhance oocyte competence. Clinical studies suggest that, while IVM still results in modestly lower cumulative live birth rates compared to conventional IVF, it offers significant advantages for selected patient populations, particularly women with polycystic ovary syndrome (PCOS), high ovarian reserve, or those requiring fertility preservation. Importantly, current evidence supports the genetic and epigenetic safety of IVM-derived offspring. As technical refinements continue and professional education expands, IVM is poised to fulfill its potential as a safer, less invasive, and more accessible alternative within the landscape of assisted reproductive technologies.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Compartment-Specific Endometrial Gene Analysis in Infertile Women with Thin Versus Normal Endometrium on Oocyte Retrieval Day: Insights from a Cross-Sectional Study. 子宫内膜薄与正常的不孕妇女在卵母细胞提取日的子宫内膜特异性基因分析：来自横断面研究的见解。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250193

Pavarit Humart, Kittima Tungprasertpol, Supatra Klaymook, Somsin Petyim

{"title":"Compartment-Specific Endometrial Gene Analysis in Infertile Women with Thin Versus Normal Endometrium on Oocyte Retrieval Day: Insights from a Cross-Sectional Study.","authors":"Pavarit Humart, Kittima Tungprasertpol, Supatra Klaymook, Somsin Petyim","doi":"10.5935/1518-0557.20250193","DOIUrl":"https://doi.org/10.5935/1518-0557.20250193","url":null,"abstract":"Objective: Thin endometrium is an increasingly recognized challenge in infertility treatment. This study aimed to compare endometrial gene expression between women with thin and normal endometrial thickness, and also to identify ovarian stimulation-related factors associated with thin endometrium.Methods: Endometrial tissue samples were collected from infertile women undergoing controlled ovarian stimulation, including 10 samples with thin endometrium (less than 7 millimeters) and 10 with normal thickness (7 millimeters or more) on the day of egg retrieval. Gene expression was analyzed using quantitative real-time polymerase chain reaction.Results: The relative expression of LGR5 in the luminal epithelium was slightly higher in the thin group, while POU5F1 expression in the stromal area was higher in the normal group. In the perivascular region, PDGFRB and SUSD2 were more highly expressed in the normal group, with SUSD2 showing a statistically significant difference (p=0.034). In addition, under higher serum estradiol levels, both POU5F1 and LGR5 showed greater expression in the normal group compared to the thin group with lower estradiol levels.Conclusions: Reduced SUSD2 expression in the perivascular region, a marker of endometrial stem and progenitor cells, may contribute to impaired endometrial growth. Serum estradiol levels also appear to influence gene expression within different endometrial compartments. These findings may offer insights into new therapeutic strategies for managing thin endometrium.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314533","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0