JBRA assisted reproduction最新文献_第2页

Clinical outcomes of 77 TESE treatment cycles in non-mosaic Klinefelter syndrome patients. 非花叶性Klinefelter综合征患者77个TESE治疗周期的临床结果

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20210081

Pedro Barros, Mariana Cunha, Alberto Barros, Mário Sousa, Sofia Dória

{"title":"Clinical outcomes of 77 TESE treatment cycles in non-mosaic Klinefelter syndrome patients.","authors":"Pedro Barros, Mariana Cunha, Alberto Barros, Mário Sousa, Sofia Dória","doi":"10.5935/1518-0557.20210081","DOIUrl":"https://doi.org/10.5935/1518-0557.20210081","url":null,"abstract":"Objective: The current study aimed to present the clinical outcomes of 76 azoospermic patients with non-mosaic Klinefelter syndrome (KS), treated with testicular spermatozoa extraction (TESE) followed by intracytoplasmic sperm injection (ICSI) using either fresh or cryopreserved testicular spermatozoa.Methods: We retrospectively evaluated 76 patients with non-mosaic KS belonging to a special group of cases that besides infertility did not present the classical signs and symptoms of testosterone deficiency. One of the patients repeated the TESE procedure (76 patients, 77 TESE cycles). Sixty of these 76 patients accepted to undergo TESE associated with ovarian stimulation, while 16 patients underwent TESE followed by testicular spermatozoa cryopreservation. Aneuploidy screening of the offspring was performed by Multiplex ligation-dependent probe amplification and by amniotic fluid karyotyping. Statistical analysis used the Chi-Squared Test, Fisher's Exact Test, 2-sided, for rates, and the Independent Samples T-test for equality of means, 2-sided.Results: Testicular spermatozoa were recovered in 31 (40.3%) of the attempts. The patients underwent 47 ICSI cycles, 25 with fresh testicular spermatozoa and 22 with cryopreserved testicular spermatozoa. Fertilization (63.5% vs. 41.6%, p=0.000), implantation (37% vs. 13.2%, p=0.014), clinical pregnancy (60.9% vs. 19%, p=0.005) and live birth (65.2% vs. 23.8%, p=0.006) rates were higher with fresh testicular spermatozoa. Chromosome analysis of the 21 newborns was normal.Conclusions: The present data adds further information regarding the recovery rate of spermatozoa after TESE and the embryological and clinical outcomes with fresh and cryopreserved testicular spermatozoa, besides reassuring the safety concerning chromosomal transmission of KS from parents to their offspring.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"412-421"},"PeriodicalIF":1.5,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355434/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39691149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Time-Lapse Embryo culture: A better understanding of embryo development and clinical application. 延时胚胎培养:更好地了解胚胎发育和临床应用。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20210107

Silvana Márquez-Hinojosa, Luis Noriega-Hoces, Luis Guzmán

引用次数: 0

The effects of morphine abuse on sperm parameters, chromatin integrity and apoptosis in men. 吗啡滥用对男性精子参数、染色质完整性和细胞凋亡的影响。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20210110

Saeed Ghasemi-Esmailabad, Amir Hossein Talebi, Ali Reza Talebi, Sepide Amiri, Mojgan Moshrefi, Majid Pourentezari

{"title":"The effects of morphine abuse on sperm parameters, chromatin integrity and apoptosis in men.","authors":"Saeed Ghasemi-Esmailabad, Amir Hossein Talebi, Ali Reza Talebi, Sepide Amiri, Mojgan Moshrefi, Majid Pourentezari","doi":"10.5935/1518-0557.20210110","DOIUrl":"https://doi.org/10.5935/1518-0557.20210110","url":null,"abstract":"Objective Morphine is one of the major psychoactive chemicals in opium that can increase the production of free radicals and thus can negatively affect spermatogenesis. The purpose of this study was to demonstrate the effects of morphine consumption on sperm parameters, DNA integrity and apoptosis in men taking morphine. Methods In this case-control study, 30 man abusing morphine (cases) and 30 healthy men (controls) were compared in sperm parameters (count, motility and morphology) and sperm chromatin quality, with Aniline Blue (AB), Toluidine blue (TB) and Chromomycin A3 (CMA3) stains. The participants were matched for age, weight, amount and duration of cigarette smoking. Results In men with morphine dependency, sperm progressive and total motility (p=0.038 and p=0.000, respectively) showed a significant decrease compared to the control group. Concerning morphine abuse, although morphine can decrease the sperm chromatin condensation and increases the rate of sperm apoptosis, these differences were not statistically significant. Conclusions According to our results morphine dependence can reduce male fertility by affecting sperm parameters.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"444-449"},"PeriodicalIF":1.5,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355429/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39882970","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

p63 expression in granulosa-luteinized cells of infertile patients with peritoneal endometriosis submitted to in vitro fertilization. 体外受精腹膜子宫内膜异位症不孕患者颗粒黄体细胞中P63的表达。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20210090

Joelmir José Chiesa, Silvia Liliana Cossio, Emily De Conto, Vanessa K Genro, João Sabino Cunha-Filho

{"title":"p63 expression in granulosa-luteinized cells of infertile patients with peritoneal endometriosis submitted to in vitro fertilization.","authors":"Joelmir José Chiesa, Silvia Liliana Cossio, Emily De Conto, Vanessa K Genro, João Sabino Cunha-Filho","doi":"10.5935/1518-0557.20210090","DOIUrl":"https://doi.org/10.5935/1518-0557.20210090","url":null,"abstract":"Objective: Endometriosis is associated with infertility, even without an anatomical abnormality. Furthermore, the peritoneal (mild) phenotype of this disease is the most prevalent and linked to infertility. The present study aimed to investigate the p63 gene and protein expression in granulosa cells from pre-ovulatory follicles in patients with endometriosis and infertility submitted to in vitro fertilization.Methods: Twenty-eight patients participated in the study and were divided into two groups according to the presence or absence of endometriosis. The p63 gene-expression levels assessment was performed by real-time PCR (qPCR) using the TaqMan assay, and we used immunofluorescence to check the p63 protein expression after IVF.Results: There was no significant difference between the groups regarding age, hormonal levels, oocyte standards, and p63 gene expression. The control group showed an RQ of 1.000 (0.431 to 2.323) and the study group showed an RQ of 0.725 (0.249 to 2.105), p>0.05. Both groups showed a weak expression of the p63 gene (p>0.05).Conclusions: This study described that endometriosis may not affect the p63 gene expression. Moreover, after follicular recruitment and growth, we found a weak expression of this protein, suggesting it is not part of oocyte maturation and development control.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"422-425"},"PeriodicalIF":1.5,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355449/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39573283","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Novel insights on premature progesterone elevation: a mini-review. 关于早孕激素升高的新见解:一个小型综述。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20210096

Alfredo Cortés-Vazquez, Cristabel Escobosa, Alfredo L Cortés-Algara, Jesús D Moreno-García

引用次数: 1

Ethics and IVF add-ons: We need to talk about it. 伦理和体外受精附加:我们需要讨论这个问题。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-08-04 DOI: 10.5935/1518-0557.20220030

Daniela Paes de Almeida Ferreira Braga, Amanda Souza Setti, Edson Borges

{"title":"Ethics and IVF add-ons: We need to talk about it.","authors":"Daniela Paes de Almeida Ferreira Braga, Amanda Souza Setti, Edson Borges","doi":"10.5935/1518-0557.20220030","DOIUrl":"https://doi.org/10.5935/1518-0557.20220030","url":null,"abstract":"In vitro fertilization (IVF) 'add-ons' are adjunct treatments used in addition to standard IVF protocols, in an attempt to improve success rates. However, the benefits for add-ons are often not supported by high-quality evidence. Nevertheless, many infertile patients are willing to try anything that might help them to improve their chances of having a baby. Therefore, the use of add-ons has been widespread and has led to extensive debate and discussion. The goal of this manuscript was to discuss the ethics underling the use of adjunct therapies in clinical practice before their safety has been thoroughly ascertained. IVF patients are routinely offered and charged for a wide range of adjunct treatments that they are told may improve their chance of a live birth, despite there being no clinical evidence supporting such efficacy. Add-on treatments are well accepted by most infertile patients, especially those who have already started their IVF treatments. A particular concern is that many clinics around the world are advertising and offering clinical adjuncts to infertile couples undergoing IVF, however, information on add-ons is often inaccurate. Data concerning the lack of scientific evidence supporting add-on efficacy and whether an add-on may cause unanticipated harm or worsen treatment outcomes is not available on most websites. IVF patients are a vulnerable population, thus there is a need for transparency about interventions for IVF, including uncertainties and risks, to support patient decision-making regarding the use of certain adjunctive therapies. Such information can be provided by clear guidelines and effective regulation.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"371-373"},"PeriodicalIF":1.5,"publicationDate":"2022-08-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9355445/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40672353","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Semen Analysis of Total Motile Sperm Count Based on the 1999 and 2010 WHO Criteria. 基于1999年和2010年WHO标准的精液总活动精子数分析。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-04-17 DOI: 10.5935/1518-0557.20210066

Patrícia de Moraes De Zorzi, Ana Paula de Souza Kussler, Anita Mylius Pimentel, Edison Capp, Helena von Eye Corleta

{"title":"Semen Analysis of Total Motile Sperm Count Based on the 1999 and 2010 WHO Criteria.","authors":"Patrícia de Moraes De Zorzi, Ana Paula de Souza Kussler, Anita Mylius Pimentel, Edison Capp, Helena von Eye Corleta","doi":"10.5935/1518-0557.20210066","DOIUrl":"https://doi.org/10.5935/1518-0557.20210066","url":null,"abstract":"Objective: Approximately 15% of the couples suffer from infertility. Half of the cases of infertility are due to male factors. Several sperm function tests have been proposed to evaluate male fertility, but sperm analysis is still the first and most important diagnostic test for male infertility. The prognostic value of semen characteristics such as concentration, morphology and motility markers are often confused with male infertility. Evaluation of seminal parameters and classification for normality remains a frequent topic of discussion.Methods: This study evaluated 477 semen samples from men undergoing investigation or infertility treatment between 2011 and 2015.Results: The spermograms of 401 patients were deemed abnormal based on the 1999 World Health Organization (WHO) criteria; the number changed to 223 when the spermograms were assessed based on the 2010 WHO criteria and to 200 when Total Motile Sperm Count (TMSC) was used as the criterion. Sperm morphology was the item in the criteria that most significantly changed spermogram classification. Normality parameters became less rigid from 1999 to 2010, thereby significantly changing the proportion of individuals no longer described as infertile/subfertile.Conclusions: The classification based on TMSC could not differentiate between fertile and infertile subjects for not taking sperm morphology into account. Nevertheless, it may be helpful in cases where intrauterine insemination is indicated.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"261-266"},"PeriodicalIF":1.5,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118975/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39631400","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of ovarian stimulation and embryonic aneuploidy in in vitro fertilization cycles with preimplantation genetic testing: A narrative systematic review. 卵巢刺激和胚胎非整倍体在体外受精周期与植入前基因检测的关联:一个叙述性系统综述。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-04-17 DOI: 10.5935/1518-0557.20210069

Jorge Rodriguez-Purata, Maria Jose Gomez-Cuesta, Enrique Cervantes-Bravo

{"title":"Association of ovarian stimulation and embryonic aneuploidy in in vitro fertilization cycles with preimplantation genetic testing: A narrative systematic review.","authors":"Jorge Rodriguez-Purata, Maria Jose Gomez-Cuesta, Enrique Cervantes-Bravo","doi":"10.5935/1518-0557.20210069","DOIUrl":"https://doi.org/10.5935/1518-0557.20210069","url":null,"abstract":"The impact of gonadotropins used for COS on the rate of embryo aneuploidy in patients without the negative effects of age as a confounding factor, is still a subject of lively debate. We ran a systematic search for studies in MEDLINE, PubMed, Google Scholar and the Cochrane Library. A librarian coordinated the search in December of 2020. We included all original peer-reviewed papers in English, irrespective of study-design. There were no restrictions concerning method of amplification or platform used to analyze the amplified DNA. We used the PICO model to select the study population. We included women/couples submitted to COS for IVF with the intention to genetically analyze her/their embryos through PGT. The primary outcome was the rate of aneuploidy. We used the Newcastle-Ottawa scale (NOS) score to evaluate the quality of the studies included. The search yielded 73 citations, and 14 were eligible for analysis, which included data on 4805 cycles. Media quality NOS score was 8. Although it has been demonstrated that natural cycles are associated with aneuploidy, it does seem that more robust stimulations are indeed associated with a higher proportion of aneuploidy. Nevertheless, a higher response is associated with an increased number of euploid embryos available for transfer, which translates into more embryo-transfer cycles with a prospective higher cumulative live birth rate. Further evidence is needed to ascertain if there is a negative impact of COS, especially at the cellular level.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"348-361"},"PeriodicalIF":1.5,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118955/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39691147","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Associations among morphological parameters, clinical factors and euploid blastocyst formation. 形态学参数、临床因素与整倍体囊胚形成的关系。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-04-17 DOI: 10.5935/1518-0557.20210008

João Paolo Bilibio, Pânila Longhi Lorenzzoni, Brenda Mendes de Oliveira, Flora Leal Nascimento, Arivaldo José Conceição Meireles, Fábio Costa do Nascimento

{"title":"Associations among morphological parameters, clinical factors and euploid blastocyst formation.","authors":"João Paolo Bilibio, Pânila Longhi Lorenzzoni, Brenda Mendes de Oliveira, Flora Leal Nascimento, Arivaldo José Conceição Meireles, Fábio Costa do Nascimento","doi":"10.5935/1518-0557.20210008","DOIUrl":"https://doi.org/10.5935/1518-0557.20210008","url":null,"abstract":"Objective: To evaluate the association among embryonic morphological parameters, clinical factors and euploid blastocyst formation.Methods: This prospective cohort study included 422 blastocysts from 135 patients who had undergone preimplantation genetic analysis after intracytoplasmic sperm injection (ICSI).Results: Of 422 blastocysts, 200 (47.4%) were euploid and 222 (52.6%) aneuploid. Women aged older than 38 years were more likely to develop aneuploid embryos (OR: 3.4, CI: 2.2-5.4, p<0.001). Poor ovarian reserve (OR: 3.3, p<0.001), increased male age (39.0 versus 40.7, p=0.019), and decrease in sperm percentage with normal morphology (2.5% vs. 1.9%, p=0.047) were associated with aneuploidy. Type C trophectoderm (TE) and type C inner cell mass were associated with a high risk of embryo aneuploidy, with OR of 4.1 (CI: 2.2-7.7, p<0.001) and 1.7 (CI: 1.01-3.0, p=0.048), respectively. Logistic regression analysis revealed maternal age and type C TE as the main risk factors for aneuploidy. Among combinations of factors, the best marker for the risk of aneuploidy was maternal age older than 38 years, combined with a type-C embryo with trophectoderm, which showed a positive predictive value of 88.6% and a specificity of 97.5%.Conclusions: Trophectoderm and type-C inner cell mass are the main embryo risk factors for aneuploidy, explaining approximately 71% and 60% of the risk, respectively. Among clinical factors, advanced maternal and paternal age (older than 38 and 36 years, respectively), antral follicles (<5), and a low percentage of sperm with normal morphology increased the risk of embryonic aneuploidy.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"199-207"},"PeriodicalIF":1.5,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://ftp.ncbi.nlm.nih.gov/pub/pmc/oa_pdf/dd/30/jbra-26-02-0199.PMC9118964.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38829308","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 2

Dual trigger improves response to ovarian stimulation and ICSI outcomes in patients with a previous r-hCG triggered ICSI cycle. 双触发改善了既往r-hCG触发ICSI周期患者对卵巢刺激和ICSI结果的反应。

IF 1.5

JBRA assisted reproduction Pub Date : 2022-04-17 DOI: 10.5935/1518-0557.20210065

Amanda Souza Setti, Luis Guilherme Louzada Maldonado, Daniela Paes de Almeida Ferreira Braga, Assumpto Iaconelli, Edson Borges

{"title":"Dual trigger improves response to ovarian stimulation and ICSI outcomes in patients with a previous r-hCG triggered ICSI cycle.","authors":"Amanda Souza Setti, Luis Guilherme Louzada Maldonado, Daniela Paes de Almeida Ferreira Braga, Assumpto Iaconelli, Edson Borges","doi":"10.5935/1518-0557.20210065","DOIUrl":"https://doi.org/10.5935/1518-0557.20210065","url":null,"abstract":"Objective: To evaluate if ovarian response to controlled ovarian stimulation (COS) and intracytoplasmic sperm injection (ICSI) outcomes are improved by the use of dual trigger (gonadotropin-releasing hormone (GnRH) agonists plus recombinant human chorionic gonadotropin (r-hCG)) in patients with previous cycles triggered with r-hCG.Methods: This case-control study included 88 matched cycles performed in 88 patients, which had the first ICSI cycle triggered with r-hCG (n=44), and the following ICSI cycle with dual trigger (n=44). We compared the cycle outcomes between the groups. In a second case-control within-subject analyses, we compared the ICSI outcomes between patients which had the first ICSI cycle triggered with r-hCG only (n=18), and the following ICSI cycle with dual trigger (n=18) or r-hCG only (n=18).Results: Upon investigating repeated cycles (r-hCG only vs. dual trigger), we found higher oocyte yield and mature oocyte rates, lower immature oocyte rates, higher fertilization rates, and higher blastocyst development rates; and higher rates of cycles with embryos transferred and implantation in the dual trigger cycle.Conclusions: The dual trigger regimen is a more effective approach than r-hCG trigger in patients with a previous r-hCG triggered ICSI cycle, yielding improved response to COS, and better laboratorial and clinical outcomes.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"255-260"},"PeriodicalIF":1.5,"publicationDate":"2022-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9118959/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"39510904","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0