JBRA assisted reproduction最新文献_第2页

Effects of Arctium lappa L. Root Powder on Some Markers of Oxidative Stress and Inflammation in Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind Controlled Clinical Trial Study. 牛蒡根粉对多囊卵巢综合征女性氧化应激和炎症标志物的影响：一项随机、双盲对照临床试验研究

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250189

Hanieh Taheri, Fatemeh Seydi, Farideh Jalali-Mashayekhi, Azam Moslemi, Heidar Farahani, Ali Khosrowbeygi

{"title":"Effects of Arctium lappa L. Root Powder on Some Markers of Oxidative Stress and Inflammation in Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind Controlled Clinical Trial Study.","authors":"Hanieh Taheri, Fatemeh Seydi, Farideh Jalali-Mashayekhi, Azam Moslemi, Heidar Farahani, Ali Khosrowbeygi","doi":"10.5935/1518-0557.20250189","DOIUrl":"https://doi.org/10.5935/1518-0557.20250189","url":null,"abstract":"Objective: Polycystic ovary syndrome (PCOS) is one of the most prevalent disorders of the endocrine system, with significant implications for female fertility. Antioxidant supplementation may contribute to its better management. This study aimed to investigate the effects of Arctium lappa L. root powder as an antioxidant on some markers of oxidative stress and inflammation, ovary volume, hirsutism score, and menstrual frequency in women with polycystic ovary syndrome.Methods: This randomized, double-blind, controlled clinical trial was conducted in 2023-2024 at Arak University of Medical Sciences. Sixty subjects with polycystic ovary syndrome were selected by convenience sampling method and allocated to Arctium lappa L. (n=30) and placebo (n=30) by permuted block randomization method and then treated with Arctium lappa L. root powder (460 mg/day) or placebo (460 mg/day) for 12 weeks. Before and after the intervention, some markers of oxidative stress and inflammation, ovary volume, hirsutism score, and menstrual frequency were measured and compared between the two groups.Results: The values of antioxidative markers, such as superoxide dismutase and catalase, increased significantly (p<0.001). Furthermore, values of oxidative and inflammation markers such as malondialdehyde and C-reactive protein decreased significantly (p<0.001) in the Arctium lappa L. compared to the placebo group. Moreover, the volume of the right and left ovaries was also reduced significantly (p=0.02, p=0.01).Conclusions: Consuming 460 mg of Arctium lappa L. root powder daily for 12 weeks can reduce ovarian volume and lower oxidative stress and inflammation in women with polycystic ovary syndrome.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Enhancing intrauterine insemination success in advanced maternal age: Impact of consecutive ejaculate and optimised cycle parameters. 提高高龄产妇宫内人工授精成功率：连续射精和优化周期参数的影响。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-02-02 DOI: 10.5935/1518-0557.20250195

Gulam Bahadur, Roy Homburg, Ralf Henkel, Kanna Jayaprakasan, Santanu Acharya, Bryan J Woodward, Asif Muneer, Judith A F Huirne, Abha Govind, Afeeza Illahibuccus, Ansam Al-Habib, Seang L Tan, Eric Jauniaux

{"title":"Enhancing intrauterine insemination success in advanced maternal age: Impact of consecutive ejaculate and optimised cycle parameters.","authors":"Gulam Bahadur, Roy Homburg, Ralf Henkel, Kanna Jayaprakasan, Santanu Acharya, Bryan J Woodward, Asif Muneer, Judith A F Huirne, Abha Govind, Afeeza Illahibuccus, Ansam Al-Habib, Seang L Tan, Eric Jauniaux","doi":"10.5935/1518-0557.20250195","DOIUrl":"https://doi.org/10.5935/1518-0557.20250195","url":null,"abstract":"Objective: This study evaluated whether consecutive ejaculate (CE) strategies improve intrauterine insemination (IUI) live birth rates (LBR) in women over 35 with unexplained or male-factor infertility. It also examined the influence of follicle number and sperm count thresholds on outcomes.Methods: In this retrospective cohort study (2010-2019), 596 IUI cycles were analysed in 263 nulliparous women-230 with CE and 366 with standard IUI. Among them, 98 patients underwent CE IUI and 165 received non-CE IUI. Patients with total motile sperm count (TMSC) <5×106 were often fast-tracked to IVF, but CE was mostly attempted to boost sperm count beforehand. LBRs per cycle and per woman were compared between groups.Results: LBR per cycle was 11.3% (CE) vs. 13.1% (control) (p=0.52); per woman, 26.5% (CE) vs. 29.1% (control) (p=0.65). Mean ages were similar (37.7 vs. 38.0 years; p=0.34). Success improved with TMSC >10×106; 65.4% (CE) and 87.5% (control). Over six cycles, LBR rose from 10.5% to 13.8% (CE) and 12.3% to 16.7% (control). Outcomes improved with two or three follicles, especially in women over 35.Conclusions: CE IUI yields LBRs comparable to standard IUI and may offer a cost-effective, less invasive alternative to IVF for male-factor infertility in women over 35. The LBRs per woman undergoing IUI were of a similar magnitude to those reported in IVF cycles. Optimising IUI LBR may involve increasing follicle numbers and using a higher TMSC threshold (>10×106). CE IUI supports healthcare sustainability while expanding fertility treatment access.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":""},"PeriodicalIF":1.9,"publicationDate":"2026-02-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

L-arginine attenuates cisplatin-induced sexual dysfunction in male Wistar rats by modulating circulating testosterone and NO/cGMP signaling. l -精氨酸通过调节循环睾酮和NO/cGMP信号通路，减轻顺铂诱导的雄性Wistar大鼠性功能障碍。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250166

O O Obembe, A A Oladipo, O Ajao, T M Akhigbe, W A Saka, R E Akhigbe

{"title":"L-arginine attenuates cisplatin-induced sexual dysfunction in male Wistar rats by modulating circulating testosterone and NO/cGMP signaling.","authors":"O O Obembe, A A Oladipo, O Ajao, T M Akhigbe, W A Saka, R E Akhigbe","doi":"10.5935/1518-0557.20250166","DOIUrl":"10.5935/1518-0557.20250166","url":null,"abstract":"Objective: Cisplatin is a highly potent and commonly used antineoplastic agent. However, it has been reported to induce male sexual dysfunction (SD) via the downregulation of testosterone and nitric oxide (NO)/ cyclic guanosine monophosphate (cGMP) signaling. On the other hand, L-arginine upregulates NO/cGMP signaling and may attenuate cisplatin-induced male sexual dysfunction. Thus, the current study examined the effect of L-arginine on cisplatin-induced male SD with a focus on testosterone bioavailability and NO/cGMP as a potential target pathway.Methods: Twenty-four male Wistar rats were allotted randomly to four groups: control, L-arginine-treated, cisplatin-treated, and cisplatin co-treatment with L-arginine.Results: Cisplatin therapy significantly lowered libido and sexual vigor, evinced by extended mount, intromission, and ejaculation latencies and reduced motivation to mate and mount, intromission, and ejaculation frequencies, as well as penile reflex. Moreover, cisplatin downregulated circulating testosterone, luteinizing hormone (LH), and follicle-stimulating hormone (FSH). In addition, cisplatin exposure markedly reduced dopamine and cavernosal levels of NO and cGMP and increased cavernosal acetylcholinesterase, monoamine oxidase, and arginase. Also, cisplatin increased cavernosal malondialdehyde, NF-kB, TNF-α, IL-1β, and IL-6 but reduced GSH, SOD, and catalase. However, co-administration of L-arginine attenuated cisplatin-induced SD by improving the indices of the male sex act and upregulating testosterone, LH, FSH, NO, cGMP, and dopamine. Arginine co-therapy also suppressed cytokine levels and improved penile redox state.Conclusions: L-arginine attenuates cisplatin-induced male SD by modulating circulating testosterone and NO/cGMP signaling.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"89-98"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055187/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145544815","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Chronic Endometritis and Recurrent Pregnancy Loss: A review of evidence and underlying mechanisms. 慢性子宫内膜炎和复发性妊娠丢失：证据和潜在机制的回顾。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250176

Fernanda Chaves Capanema Álvares, Frederico Timm Rodrigues de Sousa, Elaine Cristina Fontes de Oliveira

{"title":"Chronic Endometritis and Recurrent Pregnancy Loss: A review of evidence and underlying mechanisms.","authors":"Fernanda Chaves Capanema Álvares, Frederico Timm Rodrigues de Sousa, Elaine Cristina Fontes de Oliveira","doi":"10.5935/1518-0557.20250176","DOIUrl":"10.5935/1518-0557.20250176","url":null,"abstract":"This narrative review aims to evaluate the relationship between chronic endometritis (CE) and recurrent pregnancy loss (RPL). A comprehensive, non-systematic search on the association between RPL and CE was conducted in the PubMed, Cochrane, and SciELO databases, considering its pathophysiological mechanisms, diagnosis and pregnancy outcomes after treatment. The following terms were used as keywords: (\"chronic endometritis\" OR \"endometrial inflammation\" OR \"subclinical endometritis\") AND (\"recurrent pregnancy loss\" OR \"recurrent miscarriage\" OR \"pregnancy outcomes\"). Diagnostic hysteroscopy can be useful for identifying CE based on visual findings (e.g., edema and endometrial micropolyps). However, specificity and sensitivity vary, and it is ideally complemented by biopsy. The identification of plasma cells using the CD-138 marker is the most accurate method for diagnosing CE. Standardization is still needed for the number of plasma cells considered diagnostic and for the quality of CD-138. Tests such as next-generation sequencing and real-time PCR can identify microorganisms and aid in the development of appropriate treatments. Antibiotic regimens have shown efficacy in reducing CE and have a positive impact on pregnancy outcomes in women with RPL. In conclusion, CE appears to be a significant but often underdiagnosed contributor to RPL. Advancements in diagnostic techniques have improved the accuracy of CE identification. Evidence suggests that effective antibiotic treatment not only resolves CE but also enhances pregnancy outcomes in affected women.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"219-224"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Acute stress induction downregulates RHOX-5 gene expression causing altered fertility indices in MALE Wistar rats. 急性应激诱导下调RHOX-5基因表达导致雄性Wistar大鼠生育指标改变。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250184

Onyinye Cynthia Okeke, Olutayo Ifedayo Ajayi

{"title":"Acute stress induction downregulates RHOX-5 gene expression causing altered fertility indices in MALE Wistar rats.","authors":"Onyinye Cynthia Okeke, Olutayo Ifedayo Ajayi","doi":"10.5935/1518-0557.20250184","DOIUrl":"10.5935/1518-0557.20250184","url":null,"abstract":"Objective: Stress has been implicated in the onset and development of various diseases and conditions including cardiovascular diseases, respiratory diseases, obesity and infertility. The effects of stress on fertility were studied to elucidate possible mechanisms of stress-induced infertility.Methods: Stress was induced using the flowerpot technique. Plasma concentration of fertility hormones, inflammatory and oxidative markers, sperm profile, histological studies and molecular studies were carried out in selected tissues from the study and control groups. Statistical analysis was carried out using the GraphPad Prism 8.0.1 version. Results were presented as Mean±SEM. The Student t-test was used to compare the means obtained; and p-values lower than 0.05 were considered statistically significant.Results: Plasma concentration of testosterone, percentage of progressively motile sperm cells, percentage of normal sperm cells and relative expression of Rhox-5 gene were significantly decreased, while the C-reactive protein concentration was significantly increased in the stressed group when compared with the control group.Conclusions: Stress reduces fertility through suppressed fertility hormone secretion and reduced genetic expression of the relevant genes, causing poor-quality spermatogenesis in male Wistar rats.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"120-131"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055165/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812706","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The influence of melatonin supplementation on in vitro culture of murine embryos from polycystic ovary syndrome experimental models. 补充褪黑素对小鼠多囊卵巢综合征实验模型胚胎体外培养的影响。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250164

Marcela de Oliveira Pinheiro, Fernanda Bertuccez Cordeiro, Giuliana Camila Ramirez Ramos, Dóris Ferreira Moriyama, Gabrielle Ferrante Alves de Moraes, Karla Pacheco de Melo, Maurício de Rosa Trotta, Fernando Prado Ferreira, Edson Guimarães Lo Turco

{"title":"The influence of melatonin supplementation on in vitro culture of murine embryos from polycystic ovary syndrome experimental models.","authors":"Marcela de Oliveira Pinheiro, Fernanda Bertuccez Cordeiro, Giuliana Camila Ramirez Ramos, Dóris Ferreira Moriyama, Gabrielle Ferrante Alves de Moraes, Karla Pacheco de Melo, Maurício de Rosa Trotta, Fernando Prado Ferreira, Edson Guimarães Lo Turco","doi":"10.5935/1518-0557.20250164","DOIUrl":"10.5935/1518-0557.20250164","url":null,"abstract":"Objective: Polycystic ovary syndrome (PCOS) negatively impacts oocyte and embryo quality. However, melatonin supplementation in assisted reproduction may enhance oocyte and embryo quality. This study aimed to analyze the effect of melatonin supplementation in embryo culture, by assessing embryos quality and development in murine models.Methods: C57BL/6J mice strain were divided into 6 groups: PCOS; PCOS with melatonin; placebo; placebo with melatonin; controls; and controls with melatonin. Embryo classification was performed during all developmental phases. TUNEL assay analysis was carried out in blastocysts.Results: The melatonin supplementation showed a positive influence during cleavage and morula development for all groups. The blastocyst rate was lower in the PCOS group when compared to placebo and controls. For the TUNEL assay, placebo and control groups supplemented with melatonin had a lower number of apoptotic cells compared to their respective non-supplemented groups.Conclusions: Melatonin supplementation exerts a beneficial impact on cleavage and morula development for all groups. For PCOS, the poor blastocyst quality and high apoptosis rate emphasizes the inherent challenges associated with this condition. Melatonin potentially mitigates apoptotic events during early embryo development, suggesting its relevance as a supplementary therapeutic approach.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"56-63"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055175/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A 3PN zygote can be transferred after PGT-A and embryo ploidy analysis: A case report of a healthy neonate. PGT-A和胚胎倍性分析后可以移植3PN合子：一例健康新生儿报告。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250182

Eliana Margarita Retamoso-Paz, Marisol Martínez-Martínez, Luz Sthefany Chavarro-Tello, Clara Inés Esteban-Pérez

引用次数: 0

Investigating Outcomes of Gamete Donation in Assisted Reproductive Technology: A Retrospective Study. 辅助生殖技术配子捐献的回顾性研究。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250056

Sofia Soares, Carla Leal, Márcia Barreiro, Emídio Vale-Fernandes

{"title":"Investigating Outcomes of Gamete Donation in Assisted Reproductive Technology: A Retrospective Study.","authors":"Sofia Soares, Carla Leal, Márcia Barreiro, Emídio Vale-Fernandes","doi":"10.5935/1518-0557.20250056","DOIUrl":"10.5935/1518-0557.20250056","url":null,"abstract":"Objective: This study aims to evaluate and characterize Assisted Reproductive Technology (ART) cycles, as well as the associated obstetric and neonatal outcomes, in pregnancies resulting from gamete donation at the Public Gamete Bank of the Centro Materno Infantil do Norte Dr. Albino Aroso (CMIN), Unidade Local de Saúde de Santo António (ULSSA), in Porto, Portugal.Methods: We conducted a retrospective analysis of 238 gamete donation cycles from 2011 to 2021. Collected variables included recipient age, duration and cause of infertility, number of attempts, ART techniques employed, embryo transfer details, beta-human chorionic gonadotropin (β-hCG) values, live birth rates, gestational age, mode of delivery, and birth weight. Statistical analyses were performed using IBM SPSS Statistics 27, with a significance threshold set at p<0.05.Results: Among sperm donation cases, 29.4% resulted in preterm deliveries, with 24.3% of pregnancies being non-ongoing and 47.7% leading to cesarean sections. Low birth weight was observed in 29.3% of singletons and 69% of twins. Multiple gestations occurred in 24.5% of cases, with a significant increase when two embryos were transferred (p=0.027). In oocyte donation situations, 40% of pregnancies resulted in preterm deliveries and 43.4% experienced low birth weight. Multiple pregnancies increased the risk of preterm delivery and low birth weight.Conclusions: The high incidence of multiple gestations underscores the need for single embryo transfer, particularly for older oocyte recipients. It is crucial for couples to be educated about the associated risks and benefits of this approach, alongside the implementation of comprehensive maternal and fetal monitoring throughout the reproductive process.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"19-30"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055167/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145403676","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PGT-A in Advanced Maternal Age: The probability of pregnancy is increased? 高龄产妇的PGT-A：怀孕的可能性增加？

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250170

M Valeria Paz, Paula Hovanyecz, Patricia Perfumo, Luciana Domenech, Viviana Ventura

{"title":"PGT-A in Advanced Maternal Age: The probability of pregnancy is increased?","authors":"M Valeria Paz, Paula Hovanyecz, Patricia Perfumo, Luciana Domenech, Viviana Ventura","doi":"10.5935/1518-0557.20250170","DOIUrl":"10.5935/1518-0557.20250170","url":null,"abstract":"Objective: To determine the probability of achieving a successful pregnancy transferring a euploid embryo per follicular aspiration in women of advanced reproductive age and based on these results to provide an additional clinical tool to support patient counseling in assisted reproductive treatments.Methods: All patients undergoing follicular aspiration and requiring PGT-A from January 2016 to March 2023 were included. Patients were divided into 4 groups based on age: <35, 35-37, 38-40, and 41 or older. It was calculated for each group the percentage of aspirated cycles with at least one blastocyst for biopsy and the percentage of these cycles with at least one euploid embryo. Ongoing pregnancy rate was calculated for the first euploid embryo transfer. The probability of achieving a successful pregnancy per aspirated cycle was finally determined and it was compared between age groups with Chi-square analysis (significance level of 5%).Results: There were no significant differences in pregnancy rate per transfer across age groups when euploids embryos were transferred (p=0.61). As patient age increases, the chance of obtaining a blastocyst suitable for biopsy per PGT-A aspirated cycle, and the chance of this embryo being euploid and transferred decrease. Finally, the probability of achieving a successful pregnancy per PGT-A aspirated cycle decreases from 29% in patients younger than 35 years old, to 5% in patients over 40 years old.Conclusions: Only 5% of women aged 41 or older would achieve pregnancy by transferring a euploid embryo per follicular aspiration. Providing accurate counseling that aligns with the perspective and expectations of these patients will be a challenge for healthcare professionals, even when utilizing the PGT-A technique.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"142-145"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055160/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145544894","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A comparison of simplified versus the original media in standard vapor freezing and in-house vitrification of human sperm. 人类精子标准蒸汽冷冻和室内玻璃化冷冻的简化培养基与原始培养基的比较。

IF 1.9

JBRA assisted reproduction Pub Date : 2026-01-01 DOI: 10.5935/1518-0557.20250154

Natpat Jansaka, Ubol Saeng-Anan, Waraporn Piromlertamorn, Teraporn Vutyavanich, Usanee Samee

{"title":"A comparison of simplified versus the original media in standard vapor freezing and in-house vitrification of human sperm.","authors":"Natpat Jansaka, Ubol Saeng-Anan, Waraporn Piromlertamorn, Teraporn Vutyavanich, Usanee Samee","doi":"10.5935/1518-0557.20250154","DOIUrl":"10.5935/1518-0557.20250154","url":null,"abstract":"Objective: We compared post-cryopreserved outcomes of normozoospermic semen samples after cryopreservation by vitrification and liquid nitrogen vapor freezing, using the original and the simplified preservation media.Methods: Forty normozoospermic semen samples were used in the study. Post-prepared semen samples were divided into five aliquots: one served as non-cryopreserved control; two were vitrified using in-house (In-house-V) or sucrose media (Simp-V); and the last two aliquots were frozen in liquid nitrogen vapor, using commercial (Com-L) or sucrose media (Simp-L).Results: Sperm after cryopreservation regardless of the media and method used, significantly decreased in motility, viability, and increased ROS level without changes in sperm morphology and DNA fragmentation. Simplified sucrose freezing medium significantly increases post-thawed motility (57.6% (53.2-68.9) vs. 34.5% (27.3-43.6) in Simp-L and Com-L) and viability (61.0% (52.0-67.8) vs. 37.0% (29.3-46.0) in Simp-L and Com-L) in vapor freezing but significantly decrease post-thawed motility (58.8% (51.4-63.3) vs. 77.8% (70.8-81.3) in Simp-V and In-house-V) and viability (59.0% (53.2-66.0) vs. 75.5% (68.0-83.0) in Simp-V and In-house-V) in vitrification. A simplified medium does not affect sperm morphology, ROS level, and DNA fragmentation.Conclusions: In liquid nitrogen vapor freezing, a simplified medium significantly improved sperm motility and viability compared with commercial medium. In vitrification, the simplified medium gave inferior results on sperm motility and viability compared to the original preservation medium.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"39-46"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055179/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314538","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0