JBRA assisted reproduction最新文献

{"title":"Cancer fertility preservation: a report from a Brazilian social program.","authors":"Caroline Z Berton,&nbsp;Caroline Brogliato,&nbsp;Ivan H Yoshida,&nbsp;Laura T Vellez,&nbsp;Claudia H Suganuma,&nbsp;Emerson B Cordts,&nbsp;Gabriel S Conceição,&nbsp;Caio P Barbosa","doi":"10.5935/1518-0557.20190089","DOIUrl":"https://doi.org/10.5935/1518-0557.20190089","url":null,"abstract":"<p><strong>Objective: </strong>To present clinical and laboratory data of a Brazilian social program for cancer fertility preservation.</p><p><strong>Methods: </strong>We carried out a descriptive observational study between July 2011 and December 2018. 246 patients were included from a social program in a private assisted reproduction clinic in Santo André/Brazil for oocyte cryopreservation before starting oncological treatment.</p><p><strong>Results: </strong>246 cancer patients resorted to fertility preservation before initiating cancer treatment. These were diagnosed with 27 different types of cancer, and the breast type is the most prevalent. 2528 MII oocytes (mean of 10.3 oocytes per patient) were vitrified. Four patients thawed their oocytes to submit in vitro fertilization, three had embryos transferred and one achieved pregnancy.</p><p><strong>Conclusion: </strong>Preservation of fertility offers patients, especially at reproductive age, a viable way to perform their cancer treatment without compromising future gestation. It is important that professionals duly counsel oncological patients so, if they wish, they can have the possibility to guarantee her fertility preserved.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"302-304"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365537/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37838614","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The role played by granulocyte colony stimulating factor (G-CSF) on women submitted to in vitro fertilization associated with thin endometrium: systematic review.","authors":"Mylena Naves de Castro Rocha,&nbsp;Rodopiano de Souza Florêncio,&nbsp;Rosane Ribeiro Figueiredo Alves","doi":"10.5935/1518-0557.20200025","DOIUrl":"https://doi.org/10.5935/1518-0557.20200025","url":null,"abstract":"<p><strong>Objective: </strong>To provide evidence available in the literature on the role of granulocyte colony stimulating factor (G-CSF) in women submitted to in vitro fertilization, with repeated implantation failure associated with thin endometrium.</p><p><strong>Methods: </strong>Systematic review of the use of G-CSF, as part of assisted reproduction techniques in women with repeated embryo implantation failures associated with thin endometrium. The study was carried out in the PubMed, BIREME and Elsevier databases from 2008 to 2018, in English, Spanish and Portuguese.</p><p><strong>Results: </strong>We included all the studies, which used intrauterine G-CSF. We found an increase in endometrial thickness in eight of the 10 studies included. Of these, the implantation rate improved significantly in two studies, but the gestation rate increased in only one. We found the highest rates of implantation (32%) and pregnancy (48%) in a non-randomized clinical trial. On the other hand, two other studies did not demonstrate an increase in endometrial thickness and in pregnancy rates in patients with thin endometrium submitted to the assisted reproduction in frozen embryo transfer cycles.</p><p><strong>Conclusion: </strong>Studies published so far point to a positive influence on the use of G-CSF in relation to the improvement in endometrial receptivity and pregnancy rates. Therefore, there is a need for further studies to determine whether to use it, as well as the period, route of administration, dosage and duration of treatment.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"278-282"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365529/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37838620","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A critical analysis of the impact of endocrine disruptors as a possible etiology of primary ovarian insufficiency.","authors":"Cecilia de Souza Monteiro,&nbsp;Erica Becker de Sousa Xavier,&nbsp;João Pedro Junqueira Caetano,&nbsp;Ricardo Mello Marinho","doi":"10.5935/1518-0557.20200005","DOIUrl":"https://doi.org/10.5935/1518-0557.20200005","url":null,"abstract":"<p><p>Primary ovarian insufficiency is a cause of infertility that affects about 1% of women under 40 years old, and is considered as idiopathic in 75% of cases. This review aims to carry out a critical synthesis of the knowledge of the chemical agents likely to affect follicular stock in humans and / or animals, by direct toxicity to follicles, or by increasing their recruitments. For the majority of toxic agents, only experimental data are currently available. We propose a strategy to encourage progress in identifying occupational factors responsible for premature ovarian failure.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"324-331"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365523/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37762593","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The impact of letrozole administration on oocyte morphology in breast cancer patients undergoing fertility preservation.","authors":"Ludmila M N Bercaire,&nbsp;Mario Cavagna,&nbsp;Nilka F Donadio,&nbsp;Andressa R Rocha,&nbsp;Rafael Portela,&nbsp;Vanessa R Alves,&nbsp;Thamara B B Santos,&nbsp;Felipe Cavagna,&nbsp;Artur Dzik,&nbsp;Luiz H Gebrim,&nbsp;Eliana A P Nahas","doi":"10.5935/1518-0557.20200002","DOIUrl":"https://doi.org/10.5935/1518-0557.20200002","url":null,"abstract":"<p><strong>Abstract: </strong>Patients submitted to oncological fertility preservation with letrozole and gonadotropins seem to present a higher rate of immature oocytes and lower fertilization rates in comparison to infertile patients submitted to IVF cycles with gonadotropins. The aim of this study was to evaluate the influence of letrozole on oocyte morphology in patients with breast cancer submitted to fertility preservation.</p><p><strong>Methods: </strong>Retrospective analysis performed at a public tertiary hospital in São Paulo, Brazil. The oocytes were retrieved from patients with breast cancer undergoing fertility preservation (n=69), and from infertile women undergoing in vitro fertilization (n=92). We evaluated 750 oocytes obtained from breast cancer patients submitted to ovarian stimulation with letrozole and gonadotropins, and 699 oocytes from patients without breast cancer submitted to ovarian stimulation for in vitro fertilization with gonadotropins only due to male factor infertility. The mature oocytes retrieved were analyzed for the presence of refractile bodies, ooplasm color and regularity, central granulation degree, cortical granules, zona pellucida staining and regularity, perivitelline space, presence of vacuoles or abnormal smooth-surfaced endoplasmic reticle and oocyte retraction.</p><p><strong>Results: </strong>There was a higher incidence of alterations in oocyte morphology in the letrozole group when compared to the control group: increased perivitelline space (p=0.007), irregular zona pellucida (p<0.001), refractile bodies (p<0.001), dark ooplasm (p<0.001), granular ooplasm (p<0.001), irregular ooplasm (p<0.001) and dense central granulation (p<0.001).</p><p><strong>Conclusion: </strong>Letrozole is a risk factor for worse oocyte morphology. However, the clinical impact of ovarian stimulation protocol with combined use of gonadotropins and letrozole for fertility preservation remains unclear in this setting. These data underline the importance of establishing the predictive potential of morphological dimorphisms of human oocytes in IVF outcomes.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"257-264"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365524/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37838616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Thyroid hormones in ovarian follicular fluid: Association with oocyte retrieval in women undergoing assisted fertilization procedures.","authors":"Mónica Rosales,&nbsp;Myriam Nuñez,&nbsp;Andrea Abdala,&nbsp;Viviana Mesch,&nbsp;Gabriela Mendeluk","doi":"10.5935/1518-0557.20200004","DOIUrl":"https://doi.org/10.5935/1518-0557.20200004","url":null,"abstract":"<p><strong>Objective: </strong>Our aim was to analyze the role of thyroid hormones in follicular fluid (FF) in relation to the number of oocytes retrieved in women recruited for an assisted fertilization procedure.</p><p><strong>Methods: </strong>Retrospective cohort study of 51 women 37.5±3.3 years, range 29-42, evaluated after a controlled ovarian stimulation protocol in a University Hospital. FF was sampled by transvaginal ultrasound-guided aspiration after ovarian hyperstimulation and we measured T3 (T3f), T4 (T4f), TSH (TSHf) and free T4 (T4ff). The oocyte maturation rate was calculated as: Number of metaphase II oocytes/Number of oocytes retrieved x 100. Statistical analysis was performed using the SPSS-19 software.</p><p><strong>Results: </strong>Hormone levels in FF were: TSHf 1.3µIU/ml (0.4 - 2.7), T3f: 1.52±0.46 nmol/L, T4f 88.8±30.9nmol/L and T4ff: 15.44±2.57pmol/L. The number of oocytes recovered was dependent onT4f following the equation: Log (oocyte) = 0.379+0.042*T4f (r:0.352, p=0.012). After a logistic regression model analysis, T3f showed a tendency to be associated with the OMR: OR (95 % CI)= 0.977 (0.954 to 1.001), p=0.057.</p><p><strong>Conclusions: </strong>The correlation found between thyroid hormones and the number of oocytes retrieved suggests an interaction between thyroid and gonadal axes in relation to follicular development.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"245-249"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365548/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37723133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research priorities in infertility and assisted reproductive technology treatments - a James Lind Alliance priority setting partnership with brazilian patients.","authors":"Aline R Lorenzon,&nbsp;Désirée Garcia,&nbsp;Leticia Silva,&nbsp;Cristiane Araújo de Oliveira,&nbsp;Mauricio B Chehin,&nbsp;Ricardo Mello Marinho,&nbsp;João Pedro Junqueira Caetano,&nbsp;Rita Vassena,&nbsp;Eduardo Leme Alves da Motta","doi":"10.5935/1518-0557.20190077","DOIUrl":"https://doi.org/10.5935/1518-0557.20190077","url":null,"abstract":"<p><strong>Objective: </strong>To identify the main research interests of Brazilian patients in the field of infertility and assisted reproductive technology (ART) treatments.</p><p><strong>Methods: </strong>This prospective multicenter cross-sectional study was carried out in Brazil. Patients attending five fertility centers from the Huntington Group between October and December 2018 were invited to join the study, which consisted of answering an anonymous survey online. Two hundred and twenty-seven patients signed the informed consent form and were emailed the survey link. The survey was designed based on the James Lind Alliance Priority Setting Partnership protocol. In the area of infertility, patients were probed on issues such as somatic and psychological effects of treatment, prevention, assisted reproductive technology (medications and procedures), success rates, risks, and emotional aspects.</p><p><strong>Results: </strong>The response rate (RR) was 47.58% (108 patients; 88 women - RR 51.46% and 20 men - RR 35.71%). Patient mean age was 36.5 years (SD 4.6). The top ten research priorities listed were 1) short- and long-term side effects of treatment; 2) how to cope with infertility; 3) risks associated with ART; 4) success rates in ART; 5) impact of diet on ART and fertility; 6) healthy habits; 7) alternative therapies; 8) impact of exercise on fertility and ART success; 9) oocyte quality and ovarian reserve; and 10) genetic or inherited causes of infertility.</p><p><strong>Conclusion: </strong>To better cater to the needs of patients and develop patient-centered care in the field of infertility and ART treatment, clinicians, healthcare providers, and the scientific community must identify patient concerns and priorities and make efforts to address them.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"265-272"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365546/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37725989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting The Developmental Potential Of Cleavage Stage Embryos Based On Oxygen Consumption Rate In FET Cycles.","authors":"Shubiao Han,&nbsp;Jiying Hou,&nbsp;Xiaodong Zhang,&nbsp;Guoning Huang","doi":"10.5935/1518-0557.20190080","DOIUrl":"https://doi.org/10.5935/1518-0557.20190080","url":null,"abstract":"Objective: To study the value of oxygen consumption (OC) as a predictor of the developmental potential of D3 embryos in frozen embryo transfer (FET) cycles. Methods: This observational study included 148 patients undergoing FET cycles with two embryos transferred per cycle. OC rates were examined by scanning electrochemical microscopy before embryo transfer. Implantation, clinical pregnancy, miscarriage, and live birth rates were calculated. Results: A total of 296 embryos were transferred in 148 cycles, or two embryos per cycle. The embryos were divided into three groups based on OC: Group A included the cases in which the OC rate of each of the two transferred embryos was greater than 3.0 fmol/s; Group B included the cases in which the OC rate of one of the embryos was greater than 3.0 fmol/s and the OC rate of the other embryo was less than 3.0 fmol/s; and Group C included the cases in which the OC rates of the two embryos were less than 3.0 fmol/s. Higher live birth rates and lower miscarriage rates were observed in Group A (p<0.05). Conclusions: Our data suggest that OC is positively correlated with embryo developmental potential. Therefore, measuring the OC of human embryos may be useful in embryo assessment.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"241-244"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365525/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37656189","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sperm selection in IVF: the long and winding road from bench to bedside.","authors":"Moisa Lucia Pedrosa,&nbsp;Marcelo Horta Furtado,&nbsp;Márcia Cristina França Ferreira,&nbsp;Márcia Mendonça Carneiro","doi":"10.5935/1518-0557.20190081","DOIUrl":"https://doi.org/10.5935/1518-0557.20190081","url":null,"abstract":"Spermatozoa wage battle to conquer fertilization but the traits needed to succeed remain elusive. The natural advantageous qualities that enable only a few selected sperm cells to reach the site of fertilization remain unknown. Although in vitro fertilization (IVF) facilitates the job of spermatozoa, a universally acceptable means of sperm selection is yet to be developed. No objective or reliable sperm quality indicators have been established and sperm selection is, to a great extent, based on subjective qualitative evaluation. The best method for sperm selection in IVF presents several challenges: intrinsic sperm qualities cannot be evaluated and the ideal endpoint for these studies is debatable. An ideal method for sperm selection in ART should be noninvasive and cost-effective, and allow the identification of high-quality spermatozoa and yield better outcomes in terms of pregnancy and live birth rates. This narrative review included 85 papers and focused on the new available methods and technologies that might shed some light on sperm selection in IVF. It discusses the available data on microfluidic devices, omics profiling, micronuclei studies, sperm plasma membrane markers, and other techniques, such as Magnetic Activated Cell Sorting (MACS), Raman micro-spectroscopy, and artificial intelligence systems. The new techniques herein reviewed offer fresh approaches to an old problem, for which a definite solution has yet to cross the bridge from bench to IVF clinics around the world, since clinical usefulness and application remain unproven.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"332-339"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365522/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37723132","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Taurine induces autophagy and inhibits oxidative stress in mice Leydig cells.","authors":"Shokofeh Yahyavy,&nbsp;Armita Valizadeh,&nbsp;Ghasem Saki,&nbsp;Layasadat Khorsandi","doi":"10.5935/1518-0557.20190079","DOIUrl":"https://doi.org/10.5935/1518-0557.20190079","url":null,"abstract":"<p><strong>Objectives: </strong>This study evaluated taurine (TAU) effects on autophagy, apoptosis and oxidative stress in mice Leydig TM3 cells.</p><p><strong>Methods: </strong>We treated TM3 cells with TAU (100 µg/mL) or 3-Methyladenine (3-MA, an autophagy inhibitor) for 24 h, and assessed cell viability, testosterone level, oxidative stress, apoptosis, and autophagy.</p><p><strong>Results: </strong>The results showed that TAU markedly increased cell viability, testosterone levels, expression of autophagy-related genes and percentage of LC3-II-positive cells. TAU significantly reduced malondialdehyde (MDA) contents and reactive oxygen species (ROS) levels and increased the activities of SOD (superoxide dismutase) and CAT (Catalase) enzymes in the TM3 cells. TAU in the presence of autophagy inhibitor (3-MA) increased oxidative stress and decreased testosterone levels.</p><p><strong>Conclusion: </strong>The results showed that autophagy might be involved in TAU-increased testosterone levels in mice Leydig TM3 cells.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"250-256"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365531/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37723134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two successful spontaneous pregnancies, single and twin, in uterus bicornis unicollis after deep infiltration endometriosis surgery.","authors":"Luciene K Tsukuda,&nbsp;Aline R Lorenzon,&nbsp;Tatiana Cs Bonetti,&nbsp;Paulo Cesar Serafini,&nbsp;Eduardo LA Motta,&nbsp;Ricardo Ma Pereira,&nbsp;Thais Sanches Domingues","doi":"10.5935/1518-0557.20200019","DOIUrl":"https://doi.org/10.5935/1518-0557.20200019","url":null,"abstract":"<p><p>A 26-year-old patient was admitted in our center with one year of infertility history after a miscarriage. She was diagnosed with uterus bicornis unicollis and deep infiltrating endometriosis (DIE); therefore, she underwent endometriosis focus removal surgery. After six-months, she conceived spontaneously and delivered one healthy baby. One year after the first pregnancy delivery, she conceived spontaneously and delivered twins in an extremely rare condition of uterus bicornis unicollis, of which there are only 15 cases reported worldwide. Both pregnancies were monitored every two or three weeks using ultrasonography to assess fetal growth, and cervical length was measured to assess the risk of premature delivery.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"387-390"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365539/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"37727072","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}