JBRA assisted reproduction最新文献

{"title":"Sertoli Cells Only Syndrome - Case Report.","authors":"Ana Bartmann","doi":"10.5935/1518-0557.20200078","DOIUrl":"https://doi.org/10.5935/1518-0557.20200078","url":null,"abstract":"<p><p>Exclusive Sertoli Cell Syndrome (ESCS) is a rare condition that has male infertility as its main consequence. It is one of the most serious forms of non-obstructive azoospermia, with a poor reproductive prognosis. In some cases, however, such as the type II of the syndrome, sperm can be recovered through testicular puncture and subsequent ICSI, with a 13% success rate. This article aims to report the case of an azoospermic 35-year-old patient, with no other significant changes in complementary exams. After percutaneous puncture of the epididymis and biopsy with no sperm, we diagnosed ESCS, and indicated IVF with donor semen.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"331-323"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083846/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vitro maturation of Mus musculus mice oocytes after hyperosmotic shock induced by vitrification solutions.","authors":"Erica Koaski,&nbsp;Cláudia Schneider Colle,&nbsp;Rafael Alonso Salvador,&nbsp;Vera Lucia Lângaro Amaral,&nbsp;Alfred Paul Senn,&nbsp;David Til","doi":"10.5935/1518-0557.20200084","DOIUrl":"https://doi.org/10.5935/1518-0557.20200084","url":null,"abstract":"<p><strong>Objective: </strong>To evaluate in vitro oocyte maturation rates in embryonic culture medium after induction by hyperosmotic shock caused by exposure to vitrification solutions.</p><p><strong>Methods: </strong>Bilateral oophorectomy was performed on 20 prepubescent female mice (Swiss). Immature (Prophase I) oocytes (N = 400) were obtained by ovarian dissection, divided into 4 groups, and transferred to culture dishes containing fertilization medium (Sydney IVF Fertilization Medium, Cook® Medical). The control group (CG) did not receive treatment, the test groups (G1, G2, G3) were treated with vitrification solution - 2 (VI-2: 14 M sucrose + ethylene glycol and dimethyl sulfoxide) for 30 seconds and subsequently: G1: 30 seconds in devitrification solution - 2 (DV-2: 0.5M sucrose); G2: 60 seconds DV-2; G3: 60 seconds DV-1(1M sucrose) and 180 seconds DV-2. All groups were cultivated for 24 hours in an incubator at 37ºC and 5% CO2 (Thermo model 3110). After this period, we checked their maturation status.</p><p><strong>Results: </strong>Oocytes exposed to VI-2, DV-1 and DV-2 (G3) showed the highest rate of competence in resuming meiosis and reaching the MII stage; however, there was no statistically significant difference (G3 = 50.5% - 49/97; CG = 27.8% - 10/30).</p><p><strong>Conclusions: </strong>Oocyte exposure to vitrification solutions, in order to cause osmotic shock, did not interfere with the resumption of meiosis in mice oocytes.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"223-228"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083860/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351942","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Semen pH and its correlation with motility and count - A study in subfertile men.","authors":"Sanketh Satya Dhumal,&nbsp;Prashanth Naik,&nbsp;Swaminathan Dakshinamurthy,&nbsp;Kishan Sullia","doi":"10.5935/1518-0557.20200080","DOIUrl":"https://doi.org/10.5935/1518-0557.20200080","url":null,"abstract":"<p><strong>Objective: </strong>The current study was aimed at correlating semen pH with motility and count to understand the significance of semen buffering system.</p><p><strong>Methods: </strong>The semen samples were collected from men who visited the clinic with infertility problems. Determination of semen pH, sperm motility and count were done according to the WHO laboratory manual, 2010 standards. The Mann-Whitney U-test was applied for statistical significance. The Pearson product moment correlation coefficient was used to measure the degree of linear relationship between the semen parameters.</p><p><strong>Result: </strong>For all patients (n=310) the mean±SD pH was found to be 8.4±0.3, with a range from 6.9 to 9.5. There was a significant and positive correlation between total motility and pH r =0.0591 (p<0.00001); volume and pH r=0.0582 (p<0.00001); Sluggish Progressive Motility (SPM) and pH, r = 0.0529 (p<0.00001); Abstinence and pH, r=0.0016 (p<0.00001). Negative correlation was noted between pH and total count r= -0.025 (p<0.00001); Rapid Progressive Motility (RPM) and pH r = -0.0776 (p<0.001), Non Motile (NM) spermatozoa and pH r=- 0.00132 (p<0.00001).</p><p><strong>Conclusion: </strong>There were correlations between seminal pH and two important fertility parameters, viz., motility and count, indicating that the semen buffering system plays a vital role in maintaining the overall seminal quality, which is of clinical relevance.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"172-175"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083857/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869449","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19: A review and considerations for the resumption of activities in an IVF laboratory and clinic in Brazil.","authors":"Ianaê Ceschin,&nbsp;Taccyanna Ali,&nbsp;Cristina Carvalho,&nbsp;Mariane Uehara,&nbsp;Priscila Motta,&nbsp;Marcia Riboldi","doi":"10.5935/1518-0557.20200102","DOIUrl":"https://doi.org/10.5935/1518-0557.20200102","url":null,"abstract":"<p><p>COVID-19 has caused radical effects on the daily lives of millions of people. The causal agent of the current pandemic is SARS-CoV-2, a virus that causes symptoms related to the respiratory system, leading to severe complications. In the in vitro fertilization (IVF) universe, there are several protocols for infection control and laboratory safety. Some professional associations have issued guidelines recommending measures involving patient flow and IVF practices. This study presents a review and considerations for the resumption of activities in IVF laboratories and clinics in Brazil during the COVID-19 pandemic, according to the guidelines and statements from professional organizations and societies in reproductive medicine.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"293-302"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083870/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25469199","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Follicular Output Rate (FORT) as a method to evaluate transdermal testosterone efficacy in poor responders.","authors":"Roser Solernou,&nbsp;Sara Peralta,&nbsp;Gemma Casals,&nbsp;Marta Guimera,&nbsp;Marina Solsona,&nbsp;Aina Borras,&nbsp;Dolores Manau,&nbsp;Francesc Fàbregues","doi":"10.5935/1518-0557.20200086","DOIUrl":"https://doi.org/10.5935/1518-0557.20200086","url":null,"abstract":"<p><strong>Objective: </strong>Follicular Output Rate (FORT) is an efficient quantitative and qualitative marker of ovarian responsiveness to gonadotropins. Transdermal testosterone (TT) has been used as adjuvant therapy to gonadotrophins in order to improve ovarian response in poor responders (PR). The aim of this study was to analyze whether TT can improve follicular sensitivity to gonadotropins using FORT.</p><p><strong>Methods: </strong>This retrospective study, held in a tertiary-care university hospital included 90 PR patients, according to the Bologna criteria. Patients in Group 1 (n = 46) received transdermal application of testosterone preceding gonadotrophin ovarian stimulation under pituitary suppression. In Group 2 (n = 44) ovarian stimulation was carried out with high-dose gonadotrophin in association with minidose GnRH agonist protocol. We analyzed ovarian stimulation parameters and IVF outcomes. We determined antral follicle count (AFC) (3-8 mm) before ovarian stimulation, pre-ovulatory follicle count (PFC) (16-22 mm) and the day of hCG administration. We calculated the FORT using the PFCx100/AFC ratio.</p><p><strong>Results: </strong>Baseline characteristics and ovarian reserve parameters were similar in both groups. FORT and oocytes retrieved were significantly higher in group 1 vs group 2. There were no significant differences in pregnancy rates. In group 1 there was a significant correlation between FORT and AFC.</p><p><strong>Conclusions: </strong>This study suggests that the potential beneficial mechanism of TT in poor responder patients may be based on increasing the antral follicle sensitivity to gonadotrophin. FORT is an excellent tool to demonstrate this.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"229-234"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083864/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869447","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparison of Subcutaneous and Vaginal Progesterone Used for Luteal Phase Support in Patients Undergoing Intracytoplasmic Sperm Injection Cycles.","authors":"Saghar Salehpour,&nbsp;Nasrin Saharkhiz,&nbsp;Leila Nazari,&nbsp;Ali Sobhaneian,&nbsp;Sedighe Hosseini","doi":"10.5935/1518-0557.20200090","DOIUrl":"https://doi.org/10.5935/1518-0557.20200090","url":null,"abstract":"<p><strong>Objective: </strong>Luteal phase defect in patients undergoing assisted reproductive technology (ART) is a sign of uterine failure due to insufficient progesterone effects on the endometrium. This study aims to compare the success rate and side effects of subcutaneous progesterone and vaginal progesterone to support the luteal phase in ART cycles.</p><p><strong>Methods: </strong>In this prospective randomized study, we used the traditional intracytoplasmic sperm injection (ICSI), and we transferred one or two 4-8 cell fetuses based on the patient's age on the third day of inoculation. We started with luteal phase support from the day of oocyte recovery and the patients randomly received either a daily dose of 25mg subcutaneous progesterone (Prolutex, IBSA Switzerland) or a 400mg dose of vaginal progesterone (Cyclogest, Actoverco, United Kingdom) every 12 hours. If blood BHCG pregnancy test was positive, support for the luteal phase continued until week 10 of gestation. The measured outcomes were the clinical, chemical and ongoing pregnancy rates as well as the rate of early abortion, patients' acceptance, tolerance and satisfaction.</p><p><strong>Results: </strong>The results of the present study showed that there was no statistically significant difference between clinical, chemical and ongoing pregnancy rates - as well as the rate of early abortion, and patients' satisfaction when comparing the two treatment Groups.</p><p><strong>Conclusions: </strong>it seems that the subcutaneous form of progesterone can be used in patients who are not willing to use vaginal progesterone, with similar treatment results and patient satisfaction, when compared to vaginal progesterone.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"242-245"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083861/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25361888","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluation of follicular flushing with double lumen needle in patients undergoing assisted reproductive technology treatments.","authors":"Marcelo Marinho de Souza,&nbsp;Ana Cristina Allemand Mancebo,&nbsp;Maria do Carmo Borges de Souza,&nbsp;Roberto de Azevedo Antunes,&nbsp;Ana Luiza Barbeitas,&nbsp;Verônica de Almeida Raupp,&nbsp;Layna Almeida Barbosa da Silva,&nbsp;Flávia Siqueira,&nbsp;Ana Luisa Bruno Marinho de Souza","doi":"10.5935/1518-0557.20210009","DOIUrl":"https://doi.org/10.5935/1518-0557.20210009","url":null,"abstract":"<p><strong>Objective: </strong>The purpose of this study was to investigate the possible impact of follicular flushing on the number of oocytes retrieved and oocytes in metaphase II in patients with poor ovarian response (POR) compared to direct aspiration.</p><p><strong>Methods: </strong>This prospective, comparative, randomized single center study included 208 punctures of patients with POR, submitted to assisted reproduction technology (ART) treatments. Two groups were compared; one in which double lumen needles were used (Wallace DNS1733) for follicular flushing (n=105), and one in which single lumen needles were used (Wallace ONS1733) for direct aspiration (n=103), upon the observation of ≤ 5 follicles between 15-17 mm, ≤ 4 follicles with sizes greater than 18 mm on hCG day, and ≤ 7 recovered oocytes.</p><p><strong>Results: </strong>There were no differences in age (39.07±3.88 vs. 38.11±3.43); weight (61.73±17.53 vs. 65.96±15.44); AMH (0.63±0.59 vs. 0.94±0.97); stimulation days (9.57±1.87 vs. 10.29±2.82); estradiol levels (788.94±670.82 vs. 940.16±694.69); progesterone (617.29±319.76 vs. 561.18±486.78); or number of follicles with sizes ≥18 mm (1.84±0.95 vs. 2.07±1.09). Although gonadotropin totals (1678.28±798.52 vs. 2080.45±852.36; p=0.0008), number of aspirated oocytes (3.00±2.11 vs. 3.69±2.20; p=0.02), and number of metaphase II oocytes (2.20±1.64 vs. 2.99±1.88; p=0.02) were significantly different, oocyte / follicle ratio ≥15 mm (0.93 vs. 0.98) and metaphase II oocytes / follicles ≥15 mm (0.68 vs. 0.79) were similar in both groups. The failure to capture was 16% vs. 9.8%.</p><p><strong>Conclusions: </strong>Considering that there was no difference in the oocyte per follicle ratio, follicular flushing did not increase the number of oocytes recovered from poor responders.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"272-275"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083866/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38912527","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"High anti-Müllerian hormone levels might not reflect the likelihood of clinical pregnancy rate in IVF/ICSI treatment.","authors":"Tie-Cheng Sun,&nbsp;Shan-Jie Zhou,&nbsp;Ling-Li Song,&nbsp;Jian-Hua Li,&nbsp;Xi Chen,&nbsp;Li Tian","doi":"10.5935/1518-0557.20200094","DOIUrl":"https://doi.org/10.5935/1518-0557.20200094","url":null,"abstract":"<p><strong>Objective: </strong>To investigate if high anti-Müllerian hormone (AMH) concentration is a useful tool to predict the outcome of assisted reproductive treatment.</p><p><strong>Methods: </strong>Retrospective cohort study involving 520 patients who underwent IVF/ICSI procedures in a university hospital. We measured the serum AMH level on day 3 of the menstrual cycle. Based on AMH levels, we divided the patients into three groups as follows: low (<25th percentile) AMH group, average (25th to 75th percentile) AMH group and high (>75th percentile) AMH group. We recorded the fertilization rate (FR), the number of oocytes retrieved, the number of good quality embryos (GQEs) and the clinical pregnancy rate (CPR).</p><p><strong>Results: </strong>There was no difference between the three AMH groups in terms of maternal age, body mass index (BMI), follicle-stimulating hormone (FSH), estradiol (E2), luteinizing hormone (LH) and testosterone (T) in the IVF/ICSI cycles. The women in the high serum AMH group had a higher number of retrieved oocytes than those in the low or average AMH groups (p < 0.01) in the IVF/ICSI cycles. Compared with the low or average AMH groups, the women with high AMH levels had a higher number of good quality embryos (GQEs) in the IVF/ICSI cycles (p < 0.01). However, high AMH women had no significantly higher clinical pregnancy rate (CPR) compared to the women in the low or average AMH groups. In addition, for the prediction of CPR, the AMH levels alone were not an independent predictor of CPR for IVF and ICSI cycles in the ROC curve analysis.</p><p><strong>Conclusions: </strong>High anti-Müllerian hormone levels are an independent predictor of the number of retrieved oocytes and good quality embryos (GQEs), but might not reflect the likelihood of higher clinical pregnancy rates (CPR) in IVF/ICSI treatment.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"266-271"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083848/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25469287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GnRH agonist in association with hCG versus hCG alone for final oocyte maturation triggering in GnRH antagonist cycles.","authors":"Condesmar M de Oliveira,&nbsp;Carlos A M de Oliveira,&nbsp;Larissa L Fonseca,&nbsp;Kelly R R de Souza,&nbsp;Moacir R M Radaelli","doi":"10.5935/1518-0557.20200089","DOIUrl":"https://doi.org/10.5935/1518-0557.20200089","url":null,"abstract":"<p><strong>Objective: </strong>To analyze gonadotropin-releasing hormone (GnRH) agonist in association with human chorionic gonadotropin (hCG) (dual triggering) versus hCG alone (conventional triggering) for final oocyte maturation triggering in GnRH antagonist cycles in an unselected population of Brazilian women.</p><p><strong>Methods: </strong>This prospective case-control study involved 114 patients referred to autologous in vitro fertilization treatment between February 2018 and August 2019, recruited regardless of age, infertility factor or number of cycles. The patients were randomly allocated into two groups according to oocyte maturation triggering approach: group A (n = 48) - hCG only; and group B (n = 66) - hCG plus GnRH agonist. The main outcomes measured were the number of total and metaphase II (MII) oocytes retrieved.</p><p><strong>Results: </strong>The groups were homogenous in terms of age. There were no moderate or severe ovarian hyperstimulation syndrome events. There were no statistical differences concerning total or MII oocytes retrieved between the groups (p > 0.05). The MII/total oocyte rate was 70.9% in group A, and 74.5% in group B (p = 0.679). There was no oocyte retrieved in 2/48 patients (4.16%) in group A, 1/66 (1.5%) in group B. There were no MII oocytes in 4/48 patients (8.3%) in group A, and 2/66 (3%) in group B. Age was directly correlated to the number of total and MII oocytes retrieved (p < 0.05).</p><p><strong>Conclusions: </strong>Dual triggering was equivalent to conventional hCH triggering in terms of the number of total and MII oocytes retrieved in the general population. Further studies are necessary to ascertain dual triggering indication in selected groups of women.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"246-251"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19: New adaptation for IVF laboratory protocols.","authors":"Syed Waseem Andrabi,&nbsp;Mir Jaffar,&nbsp;Puneet Rana Arora","doi":"10.5935/1518-0557.20200054","DOIUrl":"https://doi.org/10.5935/1518-0557.20200054","url":null,"abstract":"<p><p>Severe acute respiratory syndrome coronavirus-2 (SARS-CoV-2) widely known as COVID-19 was first reported in late December 2019, in China. Since then this novel coronavirus has spread throughout the world. Our return to normal life will not take long, for we are in a phase where the COVID-19 curve is stabilizing. ART services must return to operation, since infertility is also a disease and treatment has to start. Before resuming ART treatments, it is very important to consider local and national regulations. Change is mandatory, to set us back to successful ART treatment without compromising on quality, and to minimize the spread of COVID-19 among staff and patients; and for this we need to take measured and vigilant steps.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"358-361"},"PeriodicalIF":1.5,"publicationDate":"2020-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7365532/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38098409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}