JBRA assisted reproduction最新文献

{"title":"In vitro maturation 2.0: a new era for an underdog in ART.","authors":"Marcos Iuri Roos Kulmann, Gabriella Mamede Andrade, Adriana Bos-Mikich, Norma Pagnoncelli de Oliveira, Marcelo Ferreira, Nilo Frantz","doi":"10.5935/1518-0557.20250177","DOIUrl":"10.5935/1518-0557.20250177","url":null,"abstract":"<p><p>In vitro maturation (IVM) of human oocytes, once a pioneering concept predating conventional IVF, has long remained an underutilized technique in assisted reproduction. Despite early promise, clinical adoption of IVM has been limited due to lower embryo developmental competence and live birth rates compared to IVF. However, recent advances in the understanding of oocyte physiology, including cumulus-oocyte communication, the regulatory roles of cAMP/cGMP signaling pathways, and endocrine modulation of meiotic resumption, have reignited interest in optimizing IVM protocols. Innovations such as biphasic Capacitation (CAPA) IVM systems and the use of ovarian somatic support cells (OSCs) derived from induced pluripotent stem cells (iPSCs) aim to replicate the dynamic follicular environment more accurately and enhance oocyte competence. Clinical studies suggest that, while IVM still results in modestly lower cumulative live birth rates compared to conventional IVF, it offers significant advantages for selected patient populations, particularly women with polycystic ovary syndrome (PCOS), high ovarian reserve, or those requiring fertility preservation. Importantly, current evidence supports the genetic and epigenetic safety of IVM-derived offspring. As technical refinements continue and professional education expands, IVM is poised to fulfill its potential as a safer, less invasive, and more accessible alternative within the landscape of assisted reproductive technologies.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"353-363"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318288/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314499","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of quercetin supplementation in aging mice on oocyte number, in vitro fertilization and embryo development outcomes.","authors":"Tanapak Wisetmongkolchai, Pannarai Somboonchai, Waraporn Piromlertamorn, Usanee Sanmee","doi":"10.5935/1518-0557.20260024","DOIUrl":"10.5935/1518-0557.20260024","url":null,"abstract":"<p><strong>Objective: </strong>To investigate the effects of quercetin supplementation in aging mice on the oocyte number and in vitro fertilization outcomes.</p><p><strong>Methods: </strong>Thirty-two to forty-week-old aging female mice were randomly assigned into two groups, with one receiving 0.2 mL water contains quercetin 30 mg/kg dissolve in DMSO orally via gavage for 21 consecutive days (n=38). The control group was given 0.2 mL of water containing DMSO without quercetin (n=37). In vitro fertilization was performed after exogenous ovarian stimulation. The outcome was oocyte number and quality, as indicated by oocyte fragmentation rate, fertilization rate, blastocyst formation rate, and blastocyst cell number.</p><p><strong>Results: </strong>There was no difference in the number of retrieved oocytes per mouse (17.4±2.6 and 16.2±1.7, p=0.694), oocyte fragmentation rate (30.8% and 26.7%, p=0.108), fertilization rate (43.5% and 43.1%, p=0.877) and blastocyst formation rate (32.6% and 33.9%, p=0.635) in the quercetin group compared to the control group. Likewise, the mean numbers of cells in the ICM, TE, and total cell number in blastocysts from both groups were not different.</p><p><strong>Conclusions: </strong>Dietary quercetin supplementation did not improve either the quantity or quality of oocytes. Thus, quercetin supplementation did not improve IVF outcomes in aged mice. However, future studies should incorporate oxidative stress biomarkers to improve mechanistic understanding.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":"30 2","pages":"341-345"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318276/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304635","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Assisted reproductive technologies in Latin America: the Latin American Registry, 2022.","authors":"Fernando Zegers-Hochschild, Javier A Crosby, Carolina Musri, Fanny Petermann-Rocha, Gustavo Martinez, Hitomi Nakagawa, Carlos Morente, Armando Roque, Ana Palma-Govea","doi":"10.5935/1518-0557.20260017","DOIUrl":"https://doi.org/10.5935/1518-0557.20260017","url":null,"abstract":"<p><strong>Research question: </strong>What are the trends and impact of ART interventions on the effectiveness and safety of ART performed in Latin America during 2022.</p><p><strong>Design: </strong>Retrospective collection of cycle-based multinational data obtained from ART procedures performed by 204 accredited institutions in 16 countries.</p><p><strong>Results: </strong>In total 123,265 initiated cycles resulted in 19,663 deliveries and 22,203 births. ART utilization showed great variability, from 643.3 cycles/million inhabitants in Uruguay to 28.8 in Guatemala. In autologous cycles, the proportion of women ≥40 years represents 35.1% of cycles while women <34 years represents only 18.8%. Nonetheless, the proportion of single embryo transfer (SET) increased from 42.4% in 2021 to 47.3% in 2022. Out of 22,203 babies born, 77.4% were singletons, 21.7% twins and 0.9% triplets or more. ICSI represented 85.4% of fertilization techniques and blastocyst transfer increased from 79.3% in 2021 to 85.3% in 2022. The delivery rate after fresh blastocyst elective SET (32.6%) was significantly higher than after the transfer of one FET from freeze-all cycle (25.2%) (p=0.0001). The number of aspirations leading to preimplantation genetic testing has increased 2.6 times in 6 years, and significantly increased delivery rates/transfer (p≤0.008) and reduced miscarriage at all ages (p≤0.004) in autologous cycles, but not in oocyte donation cycles. Delivery rates after fresh transfer of embryos from vitrified-warmed donated oocytes, generated similar outcome to FET/OD (p=0.5621). Perinatal mortality increased from 7.5‰ in singletons to 22.8‰ in twins.</p><p><strong>Conclusion: </strong>Systematic collection of cycle-based multinational data contributes to cooperative sustained development and helps implement evidence-based reproductive decisions.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":"30 2","pages":"401-416"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304592","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hyaluronic Acid Enriched Medium for Embryo Transfer: A Systematic Review and Meta-analysis.","authors":"Rodrigo Manieri Rocha, Maira C Ribeiro Andrade, Ionara Diniz Evangelista Santos Barcelos, Iara Gonçalves Roberto Viana, Wellington de Paula Martins","doi":"10.5935/1518-0557.20260012","DOIUrl":"10.5935/1518-0557.20260012","url":null,"abstract":"<p><p>This systematic review and meta-analysis of randomized controlled trials (RCTs) evaluated whether the use of hyaluronic acid (HA)-enriched medium for embryo transfer (ET) improves live birth rates in in-vitro fertilization (IVF) cycles. A comprehensive search of PubMed, Scopus, Web of Science, and ClinicalTrials.gov was conducted up to December 2024, without language restrictions, and only RCTs comparing HA-enriched to standard ET media were included. Risk of bias was assessed using the Cochrane tool, meta-analyses were performed using a random-effects model with the Mantel-Haenszel method, sensitivity analyses excluded studies at high risk of bias, and evidence quality was evaluated with GRADE. Of 450 records identified, 24 studies were eligible, of which 20 were included in the meta-analysis, and most studies had a high risk of bias. Eleven studies reported live birth outcomes, with 2,026 versus 2,037 participants and 803 versus 697 live births in the HA-enriched and standard medium groups, respectively. The relative risk (RR) for live birth was 1.14 (95% CI: 0.99-1.31; p=0.07), with moderate heterogeneity (I2=54%), and sensitivity analysis showed an RR of 1.06 (95% CI: 0.85-1.31; p=0.62). Clinical pregnancy rates were initially higher with HA-enriched medium (RR=1.17; 95% CI: 1.05-1.29; p=0.004), but this effect disappeared in sensitivity analysis (RR=1.05; p=0.53), and pregnancy loss showed no significant difference between groups. Previous studies had suggested a potential benefit of HA-enriched ET medium on live birth outcomes; however, in the present analysis no significant difference in live birth rates was observed between HA-enriched and standard ET media. Overall evidence quality was rated as very low due to high risk of bias, inconsistency, and imprecision, and current evidence does not support a clear benefit of HA-enriched ET medium, although ongoing trials may further clarify its role.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":"30 2","pages":"382-395"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318287/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Successful pregnancy following IVF in a woman with hypopituitarism secondary to germinoma and thrombophilia: a case report and literature review.","authors":"Teresa Gastañaga-Holguera, Vanesa Rayo-López, Isabel Campo-Gesto, Marta Calvo-Urrutia","doi":"10.5935/1518-0557.20260003","DOIUrl":"10.5935/1518-0557.20260003","url":null,"abstract":"<p><p>A 32-year-old woman with panhypopituitarism following surgical resection, chemotherapy, and radiotherapy for a pituitary germinoma underwent assisted reproductive treatment. Management involved comprehensive hormone replacement therapy, ovulation induction with exogenous gonadotropins, in vitro fertilization (IVF), and thromboprophylaxis with low molecular weight heparin due to a diagnosed thrombophilia. A fresh embryo transfer resulted in a successful singleton pregnancy. The pregnancy progressed uneventfully and reached full term and a healthy neonate was delivered. This case highlights the feasibility of pregnancy in women with complex endocrine and thrombotic conditions and adds to the limited literature supporting IVF in women with significant hypothalamic-pituitary axis compromise.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":"30 2","pages":"419-423"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318277/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148304591","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Arctium lappa L. Root Powder on Some Markers of Oxidative Stress and Inflammation in Women with Polycystic Ovary Syndrome: A Randomized, Double-Blind Controlled Clinical Trial Study.","authors":"Hanieh Taheri, Fatemeh Seydi, Farideh Jalali-Mashayekhi, Azam Moslemi, Heidar Farahani, Ali Khosrowbeygi","doi":"10.5935/1518-0557.20250189","DOIUrl":"10.5935/1518-0557.20250189","url":null,"abstract":"<p><strong>Objective: </strong>Polycystic ovary syndrome (PCOS) is one of the most prevalent disorders of the endocrine system, with significant implications for female fertility. Antioxidant supplementation may contribute to its better management. This study aimed to investigate the effects of Arctium lappa L. root powder as an antioxidant on some markers of oxidative stress and inflammation, ovary volume, hirsutism score, and menstrual frequency in women with polycystic ovary syndrome.</p><p><strong>Methods: </strong>This randomized, double-blind, controlled clinical trial was conducted in 2023-2024 at Arak University of Medical Sciences. Sixty subjects with polycystic ovary syndrome were selected by convenience sampling method and allocated to Arctium lappa L. (n=30) and placebo (n=30) by permuted block randomization method and then treated with Arctium lappa L. root powder (460 mg/day) or placebo (460 mg/day) for 12 weeks. Before and after the intervention, some markers of oxidative stress and inflammation, ovary volume, hirsutism score, and menstrual frequency were measured and compared between the two groups.</p><p><strong>Results: </strong>The values of antioxidative markers, such as superoxide dismutase and catalase, increased significantly (p<0.001). Furthermore, values of oxidative and inflammation markers such as malondialdehyde and C-reactive protein decreased significantly (p<0.001) in the Arctium lappa L. compared to the placebo group. Moreover, the volume of the right and left ovaries was also reduced significantly (p=0.02, p=0.01).</p><p><strong>Conclusions: </strong>Consuming 460 mg of Arctium lappa L. root powder daily for 12 weeks can reduce ovarian volume and lower oxidative stress and inflammation in women with polycystic ovary syndrome.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"272-281"},"PeriodicalIF":1.9,"publicationDate":"2026-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318290/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147314496","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of seminal cryopreservation on embryo kinetics. Image analysis and clinical outcomes.","authors":"M C Lorenzo Rodríguez, M Martínez Morales, I Pérez Cano, P Hernández Vargas, B Gadea Navarro, M Roldán Ramírez, B Amorocho Llanos, A Doménech Vidal, M A Valera Cerdá, M Muñoz Cantero, M Meseguer Escrivá","doi":"10.5935/1518-0557.20260005","DOIUrl":"10.5935/1518-0557.20260005","url":null,"abstract":"<p><strong>Objective: </strong>This study aims to compare the effect of using fresh ejaculated sperm versus cryopreserved sperm on the morphology and kinetics of embryo development and on clinical reproductive outcomes.</p><p><strong>Methods: </strong>Retrospective, observational, single-centre study conducted at IVIRMA Alicante (June 2013-December 2020) that included 469 oocyte donation cycles with frozen, fresh and mixed eggs in which ICSI, IVF or IVF-ICSI with fresh ejaculated and frozen ejaculated partner sperm was performed. For each group, the morphokinetic variables t2, t3, t4, t5, t6, t7, t8, tM, tSB, tB, tEB were studied in addition to the clinical pregnancy rate, implantation rate, ongoing pregnancy rate, miscarriage rate, and live birth rate, per transfer and per embryo transferred. Statistical analysis was performed using the IBM SPSS® software. Values of p≤0.05 were considered statistically significant.</p><p><strong>Results: </strong>Longer development times were observed in the fresh semen group, with significantly longer times for t2, t3, t4, t5, tB, tEB. Clinical results per transfer showed a trend, although not significant, towards lower rates in the cryopreserved semen group compared to the fresh semen group. However, the miscarriage rate was higher for the cryopreserved semen group, although this difference was not statistically significant.</p><p><strong>Conclusions: </strong>Our results indicate that embryos from fresh semen have a slower developmental rate for the morphokinetic parameters studied. Since no significant differences in clinical reproductive outcomes were found, it is concluded that sperm freezing is a valuable technique that can continue to be applied. However, further studies are needed to confirm these results.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"258-267"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318300/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477893","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The scientific gap in reproductive immunology and the financial burden on patients: a critical review.","authors":"Pedro Augusto Araujo Monteleone","doi":"10.5935/1518-0557.20250191","DOIUrl":"10.5935/1518-0557.20250191","url":null,"abstract":"","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"417-418"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318295/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effectiveness of BMMSC and Dienogest on IL-1β, IL-8 Expression and Lesion Area in Endometriosis Mice.","authors":"Rista Silvana, Yusuf Effendi, Rizani Amran, Widjiati Widjiati","doi":"10.5935/1518-0557.20260001","DOIUrl":"10.5935/1518-0557.20260001","url":null,"abstract":"<p><strong>Objective: </strong>Endometriosis is a chronic estrogen-dependent inflammatory disease that impairs fertility and quality of life. Dienogest, the current standard therapy, provides symptom relief but does not correct immune imbalance or prevent recurrence. Bone marrow-derived mesenchymal stem cells (BMMSCs), with strong immunomodulatory potential, may represent a disease-modifying alternative. This study evaluated the effects of BMMSCs, Dienogest, and their combination on IL-1β and IL-8 expression and lesion size in a murine endometriosis model.</p><p><strong>Methods: </strong>The experiment was conducted between August and December 2024 using 32 female mice, randomly assigned into four groups: untreated endometriosis controls (K+), BMMSC monotherapy (P1), BMMSC combined with Dienogest (P2), and Dienogest monotherapy (P3). Mice were injected intraperitoneally with endometrial fragments (200 μL) to become endometriosis models. On day 15, the models received mesenchymal stem cells. Sample collection was on day 29. Cytokine expression (IL-1β and IL-8) was assessed immunohistochemically, while lesion dimensions were analyzed using ImageJ v1.54 (NIH, USA) software. Immunofluorescence confirmed the BMMSC phenotype (CD73+, CD90+, CD105+, CD45-). Statistical analysis used one-way ANOVA and Kruskal-Wallis with Bonferroni correction.</p><p><strong>Results: </strong>BMMSC monotherapy resulted in the most pronounced suppression of IL-1β and IL-8 expression, alongside a significant reduction in lesion size (p<0.001). All treatment groups differed significantly from the control group.</p><p><strong>Conclusions: </strong>BMMSC monotherapy in endometriosis mice models markedly reduced IL-1β and IL-8 expression, decreased lesion size, and demonstrated more robust disease-modifying activity compared to Dienogest or combination therapy.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"302-313"},"PeriodicalIF":1.9,"publicationDate":"2026-03-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13318296/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147477882","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Chronic Endometritis and Recurrent Pregnancy Loss: A review of evidence and underlying mechanisms.","authors":"Fernanda Chaves Capanema Álvares, Frederico Timm Rodrigues de Sousa, Elaine Cristina Fontes de Oliveira","doi":"10.5935/1518-0557.20250176","DOIUrl":"10.5935/1518-0557.20250176","url":null,"abstract":"<p><p>This narrative review aims to evaluate the relationship between chronic endometritis (CE) and recurrent pregnancy loss (RPL). A comprehensive, non-systematic search on the association between RPL and CE was conducted in the PubMed, Cochrane, and SciELO databases, considering its pathophysiological mechanisms, diagnosis and pregnancy outcomes after treatment. The following terms were used as keywords: (\"chronic endometritis\" OR \"endometrial inflammation\" OR \"subclinical endometritis\") AND (\"recurrent pregnancy loss\" OR \"recurrent miscarriage\" OR \"pregnancy outcomes\"). Diagnostic hysteroscopy can be useful for identifying CE based on visual findings (e.g., edema and endometrial micropolyps). However, specificity and sensitivity vary, and it is ideally complemented by biopsy. The identification of plasma cells using the CD-138 marker is the most accurate method for diagnosing CE. Standardization is still needed for the number of plasma cells considered diagnostic and for the quality of CD-138. Tests such as next-generation sequencing and real-time PCR can identify microorganisms and aid in the development of appropriate treatments. Antibiotic regimens have shown efficacy in reducing CE and have a positive impact on pregnancy outcomes in women with RPL. In conclusion, CE appears to be a significant but often underdiagnosed contributor to RPL. Advancements in diagnostic techniques have improved the accuracy of CE identification. Evidence suggests that effective antibiotic treatment not only resolves CE but also enhances pregnancy outcomes in affected women.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"219-224"},"PeriodicalIF":1.9,"publicationDate":"2026-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13055183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145812675","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}