JBRA assisted reproduction最新文献

{"title":"Correlation between sperm DNA fragmentation index and semen parameters in 418 men seen at a fertility center.","authors":"Láyonal Germán Acosta Campos,&nbsp;Lissett Chiscul Requejo,&nbsp;Carlos Antonio Rivas Miñano,&nbsp;Jheny Díaz Orrego,&nbsp;Elmer Chávez Loyaga,&nbsp;Luis Gonzales Cornejo","doi":"10.5935/1518-0557.20200079","DOIUrl":"https://doi.org/10.5935/1518-0557.20200079","url":null,"abstract":"Objective: The aim of this study was to determine the correlation between semen parameters and sperm DNA fragmentation index (DFI) in 418 men who attended a fertility center. Methods: This retrospective study includes 418 male patients seen at In Vitro Gestar, Chiclayo - Perú between October 2013 and March 2020. DFI was determined between normozoospermic patients (NORMO) and patients with one altered parameter (ALTERED). The patients were classified as oligozoospermic [Severe/Moderate oligozoospermia (OLIGO SM): <5x106 sperm/mL and Mild oligozoospermia (OLIGO M): 5 to <15x106 sperm/mL], asthenozoospermic [Severe asthenozoospermia (ASTHENO S): <10% PM (progressive motility); Moderate asthenozoospermia (ASTHENO MO): 10 to <20% PM; Mild asthenozoospermia (ASTHENO M): 20 to <32% PM)], teratozoospermic (TERATO) and oligoasthenoteratozoospermic (OAT). DFI was determined between these groups and NORMO. The data was analyzed using the SPSS 22.0 software for Windows. Results: Normozoospermic patients presented lower significant levels of DFI compared with all groups. NORMO (15.64±7.65) vs [ALTERED (18.41±9.43, p=0.003); OLIGO SM (26.38±12.94, p<0.005); ASTHENO S (23.09±11.45, p<0.01); TERATO (17.96±9.23, p<0.05) and OAT (22.05±12.15, p=0.001)]. We determined a significant negative correlation between DFI and progressive motility (r= -0.162 p=0.001), and those with normal morphology (r= -0.100 p=0.040). Likewise the DFI shows a significant positive correlation with age (r= 0.257 p=0.000). Conclusions: Our study establishes that high DFI is accompanied by a significant impairment in all seminal parameters.","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"349-357"},"PeriodicalIF":1.5,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312297/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25405248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Oocyte Morphology and Reproductive Outcomes - Case Report and Literature Review.","authors":"Raquel Meirelles Guimarães,&nbsp;Larissa Maciel Ribeiro,&nbsp;Lizandra Paravidine Sasaki,&nbsp;Hitomi Miura Nakagawa,&nbsp;Iris Oliveira Cabral","doi":"10.5935/1518-0557.20210001","DOIUrl":"https://doi.org/10.5935/1518-0557.20210001","url":null,"abstract":"<p><p>Oocyte quality could be negatively affected by many factors including smoking, alcohol consumption, obesity, woman's age, endometriosis and controlled ovarian stimulation (COS), during assisted reproductive technology (ART), in addition to genetic factors, such as hormone receptor polymorphisms, for example. We know that the increase in the reactive oxygen species (ROS) due to systemic disorders causes biochemical and morphological changes to the oocytes, interfering with their quality. The oocyte dysmorphism can be expressed through intra and/or extra cytoplasmic changes. In general, the size and number of oocytes' morphological abnormalities are directly related to preimplantation development failure. This case report is based on four in vitro fertilization (IVF) cycles performed by a patient with oocyte dysmorphism in all oocytes captured. The literature review on this topic aims to relate the characteristics of the oocytes, presented in the case report, with research results about the quality and morphology of the oocytes.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"500-507"},"PeriodicalIF":1.5,"publicationDate":"2021-07-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8312307/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25495033","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Aqueous Extract of Adansonia digitata reversed Cotton Seed Extract-Induced Testicular Damage in Wistar rats.","authors":"Dare Joseph Babatunde,&nbsp;Olaniyan Olugbemi Tope,&nbsp;Oigiagbe Eboselume Fidelis,&nbsp;Okotie Gloria Enevwo,&nbsp;Eweoya Olugbenga","doi":"10.5935/1518-0557.20200092","DOIUrl":"https://doi.org/10.5935/1518-0557.20200092","url":null,"abstract":"<p><strong>Objective: </strong>Infertility is the inability of sexually active couples without using birth control to get pregnant after one year of uninterrupted sexual intercourse. Cotton Seed Extract (CSE) has been linked to male infertility by causing oxidative damage to the testes due to the action of its active component, Gossypol. Adansonia digitata has been known to have many medically useful properties, including antioxidant effects. This study aimed at evaluating the effects of Adansonia digitata on Cottonseed extract-induced testicular damage.</p><p><strong>Methods: </strong>Forty (40) Adult male Wistar rats were divided into 8 groups of five rats per group (n=5). Group 1 served as the control and received 0.5 ml of phosphate buffer orally; Group 2 received 800 mg/kg b.wt A. digitata orally; Group 3 received 300 mg/kg b.wt Vitamin E only orally; Group 4 received 60 mg/kg b.wt CSE intraperitoneally; Group 5 received 20 mg/kg b.wt CSE intraperitoneally; Group 6 received 60 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 7 received 20 mg/kg b.wt CSE intraperitoneally and 800 mg/kg b.wt A. digitata orally; Group 8 received 60 mg/kg b.wt CSE intraperitoneally and 300 mg/kg Vit. E orally. It was administered for 21 days. The testes and epididymis were dissected following abdominal incision. The epididymis was used for semen analysis while the testes was processed for histological analysis and biochemical assay. All the data was analyzed by ANOVA, using the SPSS version 17.0 software. A p<0.05 was considered significant.</p><p><strong>Results: </strong>CSE administration caused significant (p<0.05) decrease in sperm count, found in the group treated with CSE only. However, the Administration of A. digitata caused significant increase (p<0.05) in sperm count, G6PDH, LDH, GPx and SOD; however, MDA levels were decreased. Histological observations showed a decrease in the number of Spermatogonia and differentiating cells in the testes of rats treated with CSE.</p><p><strong>Conclusions: </strong>The results obtained revealed the antioxidant ability of A. digitata in counter-acting the testicular damage caused by CSE administration.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"257-265"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083865/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351945","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"COVID-19 impact on reproduction and fertility.","authors":"Akash S Mali,&nbsp;Madhuri Magdum,&nbsp;Jiri Novotny","doi":"10.5935/1518-0557.20200103","DOIUrl":"https://doi.org/10.5935/1518-0557.20200103","url":null,"abstract":"<p><p>The COVID-19 pandemic is an unexpected worldwide situation, and all countries have implemented their own policies to curb the spread of the virus. The pathophysiology of COVID-19 has opened numerous hypotheses of functional alterations in different physiological aspects. The direct impact of SARS-CoV-2 on the urogenital organs of males and females is still to be assessed. Nevertheless, based on biological similarities between SARS-CoV and SARS-CoV-2, several hypotheses have been proposed. In this study, we will discuss the possible mechanism of action, and potential effects on the male/female reproductive system and fertility.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"310-313"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083847/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"One follicle, one egg, one embryo: a case-report of successful pregnancy obtained from a single oocyte collected.","authors":"Romualdo Sciorio,&nbsp;Elisavet Angelaki,&nbsp;Naser Al-Azemi,&nbsp;Abubakr Elmardi","doi":"10.5935/1518-0557.20200087","DOIUrl":"https://doi.org/10.5935/1518-0557.20200087","url":null,"abstract":"<p><p>In this report, we present an unusual case of a couple who achieved a successful pregnancy by ICSI with a single oocyte collected. The cumulus-oocyte-complex (COC) was retrieved at 36.5 hours post trigger, and was found to be at metaphase II, when sperm injection was performed at around 39 hours post trigger. At 18 hours post injection, the single oocyte was fertilized, developed to four-cell embryo on day 2, and 8-cell grade on day 3, when it was relocated in the uterine cavity. The pregnancy yielded a positive β-hCG result. The scan performed at seven weeks, revealed the presence of one amniotic sac with a fetal heartbeat. The ongoing pregnancy has been eventless at 24 weeks of gestation.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"314-317"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083856/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869448","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Xylopia aethiopica ethanol seed extract suppresses Cadmium chloride-induced ovary and gonadotropins toxicity in adult female Wistar rats.","authors":"Elvis T Godam,&nbsp;Olugbemi T Olaniyan,&nbsp;Chiwendu D Wofuru,&nbsp;Clinton D Orupabo,&nbsp;Kenneth S Ordu,&nbsp;Barinua K Gbaranor,&nbsp;Progress D Dakoru","doi":"10.5935/1518-0557.20200091","DOIUrl":"https://doi.org/10.5935/1518-0557.20200091","url":null,"abstract":"<p><strong>Objective: </strong>Xylopia aethiopica is a common plant in West Africa, with wide applications in trado-medical management of several diseases. Thus, our study aimed to analyze the histology and hormonal effects of ethanol extracts of Xylopia aethiopica seeds on cadmium chloride-induced reproductive dysfunction in female Wistar rats.</p><p><strong>Methods: </strong>We used twenty-five rats weighing 120-150g for this study. The rats were divided into five groups (n=5). Group 1: received only distilled water orally; Group 2: received 2 mg/kg cadmium chloride orally; Group 3: received 2 mg/kg cadmium chloride plus 50 mg/kg Xylopia aethiopica seeds orally; Group 4: received 2 mg/kg cadmium chloride plus 100 mg/kg Xylopia aethiopica seeds orally, and Group 5: received 100 mg/kg Xylopia aethiopica seeds only, orally. We administered the extracts for 14 days, after which we slaughtered the animals following chloroform anesthesia. We took the blood samples by cardiac puncture for hormonal assay. The ovaries and uterus were harvested for histology. We analyzed the data using ANOVA, and the differences in mean values were considered significant at p<0.05.</p><p><strong>Results: </strong>The body weight of the rats showed a dose-dependent reduction (p<0.05), compared with the controls. Xylopia aethiopica seeds significantly (p<0.05) reversed the detrimental effects of Cadmium on LH and FSH. The histological analysis of the ovary showed significant improvement upon treatment with Xylopia aethiopica extract in a dose-dependent manner.</p><p><strong>Conclusions: </strong>The ameliorative effects of Xylopia aethiopica against cadmium chloride-induced reproductive toxicity in female Wistar rats may be attributed to its antioxidant properties.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"252-256"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083854/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869889","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ongoing pregnancy and healthy live births following very short ovarian stimulation of incidentally observed big antral follicles in oligoamenorrheic patients with extremely decreased ovarian reserve.","authors":"Volkan Turan,&nbsp;Meltem Sonmezer,&nbsp;Murat Sonmezer","doi":"10.5935/1518-0557.20200095","DOIUrl":"https://doi.org/10.5935/1518-0557.20200095","url":null,"abstract":"<p><p>In the present case series our aim is to present seven patients with extremely decreased ovarian reserve and oligomenorrhea, conceived with in vitro fertilization following a very short ovarian stimulation of incidentally detected big antral follicles. The study included women pursuing in vitro fertilization due to premature ovarian failure risk. When an incidental growing antral follicle was detected under ultrasound, immediate ovarian stimulation was initiated if the blood estradiol, luteinizing hormone and progesterone levels were correlated. Serum anti-Mullerian hormone measurements of all patients were consistent with extremely diminished ovarian reserve (ranged between 0.01 and 0.09ng/ml) and FSH levels varied between 13-104IU/l. The mean stimulation length ranged between 2-4 days. A total of 8 oocytes were retrieved; 6 MII, 1 GV and 1 degenerated. All 6 MII oocytes were fertilized with intracytoplasmic sperm injection. Two patients conceived after fresh embryo transfer, whereas the one conceived following frozen thawed embryo transfer. The ongoing pregnancy rate was 50% per transfer, and two of them resulted in a healthy live birth. In conclusion, close monitoring of oligoamenorrheic infertile patients who are at high risk of imminent ovarian failure using ultrasound and blood hormone levels is very important. Albeit low, the possibility of having a healthy pregnancy following \"a very short ovarian stimulation\" in such a specific patient group is emphasized.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"324-327"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083869/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869446","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The effects of ozone and melatonin on busulfan-induced testicular damage in mice.","authors":"Mahin Taheri Moghadam,&nbsp;Reza Dadfar,&nbsp;Layasadat Khorsandi","doi":"10.5935/1518-0557.20200081","DOIUrl":"https://doi.org/10.5935/1518-0557.20200081","url":null,"abstract":"<p><strong>Objective: </strong>Busulfan is one of the most common chemotherapeutic drugs and has the ability to induce apoptosis in testicular germ cells, which leads to infertility. In this study, the effects of ozone therapy and melatonin were evaluated on testicular disorders induced by busulfan.</p><p><strong>Methods: </strong>In this study, we divided 24 male mice into four groups: control group, groups treated with busulfan, busulfan/melatonin, and busulfan/ozone. At the end of a 35-day period, blood samples were taken from the mice and their testosterone levels were measured. Both of the mice's testes were removed and weighed, afterwards, each one of them was used for evaluation of morphology by Johnson's score, as well as for measuring the diameter and thickness of seminiferous tubules. The other testis was homogenized for measuring Malondialdehyde (MDA) and antioxidant status using Catalase (CAT), Super Oxide Dismutase (SOD), and Total Antioxidant Capacity (TAC) levels. Epididymis spermatozoa were also used to evaluate motility, morphology, and sperm count.</p><p><strong>Results: </strong>Busulfan significantly reduced the testis quality (weight, sperm parameters, testosterone, CAT, SOD, and TAC levels) and increased MDA and destruction of seminiferous tubules compared to the control group. Ozone and melatonin treatments significantly increased testis quality, sperm parameters, MDA, and antioxidant status, but they did not affect the TAC level.</p><p><strong>Conclusions: </strong>This study showed that similar to melatonin, ozone can reduce the effect of busulfan toxicity on mice testis. However, further studies are needed to understand the precise mechanism of ozone function on testis.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"176-184"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083863/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"38869450","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Long-term outcome of ovarian function after drug-free in vitro activation (IVA) in primary ovarian insufficiency patient.","authors":"Janisse Ferreri,&nbsp;Marta Méndez,&nbsp;José Maria Calafell,&nbsp;Francesc Fábregues","doi":"10.5935/1518-0557.20200093","DOIUrl":"https://doi.org/10.5935/1518-0557.20200093","url":null,"abstract":"<p><p>Drug-Free IVA has been recently introduced as a therapeutic option for patients with Primary Ovarian Insufficiency (POI). Despite the existing limited results, it can be considered as a promising option for these patients to achieve their own offspring. Here we report the case of a 35-year-old woman diagnosed with POI at 30 years of age. Drug-Free IVA was performed at age 33 and pregnancy was achieved by IVF 10 months after grafting. Unfortunately, she had a preterm delivery with neonatal death due to prematurity complications. After delivery, she recovered spontaneous ovarian function and one mature oocyte was retrieved 20 months after Drug-Free IVA. Following IVF, one embryo was transferred, and she is currently 33 weeks pregnant, suggesting that Drug-free IVA could lead to long-term ovarian function.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"318-320"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083853/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351992","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Final destination of surplus cryopreserved embryos. What decision should be made?","authors":"Carlos Wilson Dala Paula Abreu Abreu,&nbsp;Maria Lúcia Andrade Abreu,&nbsp;Maria Mariana Andrade Abreu,&nbsp;João Pedro Andrade Abreu,&nbsp;Luiz Fernando Cal Silva,&nbsp;Ines Katerina Damasceno Cavallo Cruzeiro,&nbsp;Rui Manuel Lopes Nunes","doi":"10.5935/1518-0557.20200085","DOIUrl":"https://doi.org/10.5935/1518-0557.20200085","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to evaluate what data exists in the literature about the fate of SCE.</p><p><strong>Methods: </strong>This is an integrative review of papers published in the last ten years, in the Medline and PubMed databases on the subject.</p><p><strong>Results: </strong>the studies included in this review demonstrate that the difficulties in defining the fate of surplus cryopreserved embryos are mainly due to the absence of specific legislation in most countries. Sociocultural and demographic factors such as religion, treatment period, ethnicity, income, marital status, economic status and education level are factors that influence the choice of the final destination of ECE. This study demonstrated that in most of the countries evaluated, the law or regulations do not provide clear guidance on the final destination of the surplus cryopreserved embryos, although it is reasonable to consider that the law will treat embryos, after a certain period of time, as abandoned. Accurate information about the desired destination of the surplus cryopreserved embryos is needed from the beginning of the breeding process to minimize future problems.</p><p><strong>Conclusions: </strong>We conclude that this is a controversial issue, involving ethical, legal, moral and financial issues, that lack specific legislation in almost all of the countries evaluated, which contributes to indecision, abandonment, and it hinders the final destination of these embryos.</p>","PeriodicalId":520656,"journal":{"name":"JBRA assisted reproduction","volume":" ","pages":"276-281"},"PeriodicalIF":1.5,"publicationDate":"2021-04-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8083851/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"25351989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}