The effects of morphine abuse on sperm parameters, chromatin integrity and apoptosis in men.

IF 1.9

JBRA assisted reproduction Pub Date : 2022-08-04 DOI:10.5935/1518-0557.20210110

Saeed Ghasemi-Esmailabad, Amir Hossein Talebi, Ali Reza Talebi, Sepide Amiri, Mojgan Moshrefi, Majid Pourentezari

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引用次数: 3

Abstract

Objective Morphine is one of the major psychoactive chemicals in opium that can increase the production of free radicals and thus can negatively affect spermatogenesis. The purpose of this study was to demonstrate the effects of morphine consumption on sperm parameters, DNA integrity and apoptosis in men taking morphine. Methods In this case-control study, 30 man abusing morphine (cases) and 30 healthy men (controls) were compared in sperm parameters (count, motility and morphology) and sperm chromatin quality, with Aniline Blue (AB), Toluidine blue (TB) and Chromomycin A3 (CMA3) stains. The participants were matched for age, weight, amount and duration of cigarette smoking. Results In men with morphine dependency, sperm progressive and total motility (p=0.038 and p=0.000, respectively) showed a significant decrease compared to the control group. Concerning morphine abuse, although morphine can decrease the sperm chromatin condensation and increases the rate of sperm apoptosis, these differences were not statistically significant. Conclusions According to our results morphine dependence can reduce male fertility by affecting sperm parameters.

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吗啡滥用对男性精子参数、染色质完整性和细胞凋亡的影响。

目的:吗啡是鸦片中主要的精神活性物质之一，能增加自由基的产生，从而对精子发生产生负面影响。本研究的目的是证明吗啡摄入对服用吗啡的男性精子参数、DNA完整性和细胞凋亡的影响。方法:采用苯胺蓝(AB)、甲苯胺蓝(TB)和色霉素A3 (CMA3)染色，对30例吗啡滥用男性(病例)和30例健康男性(对照)的精子参数(数量、活力、形态)和精子染色质质量进行比较。参与者的年龄、体重、吸烟量和吸烟时间都是匹配的。结果:吗啡依赖男性精子进步数和总活动力较对照组显著降低(p=0.038, p=0.000)。在吗啡滥用方面，吗啡虽能降低精子染色质凝结，增加精子凋亡率，但差异无统计学意义。结论:吗啡依赖可通过影响精子参数降低男性生育能力。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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JBRA assisted reproduction

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