Materials Science & Engineering C-Materials for Biological Applications最新文献

{"title":"Advanced intranasal peptide delivery systems for improved management of Alzheimer's disease","authors":"Ankit Majie ,&nbsp;Varnita Karmakar ,&nbsp;Arya Ghosh ,&nbsp;Snigdha Chakraborty ,&nbsp;Apurva ,&nbsp;Buddhadev Layek ,&nbsp;Bapi Gorain","doi":"10.1016/j.bioadv.2025.214474","DOIUrl":"10.1016/j.bioadv.2025.214474","url":null,"abstract":"<div><div>Alzheimer's disease (AD) is a progressive neurodegenerative disorder that leads to cognitive decline, memory loss, and impairment in daily functioning, making up nearly 60 % of all dementia cases. Current treatments primarily manage symptoms rather than address the disease itself, underscoring the need for more effective solutions. Therapeutic peptides have emerged as promising candidates, targeting critical pathological processes in AD. Additionally, intranasal delivery offers significant advantages, including non-invasiveness, enhanced stability, rapid absorption, and the ability to bypass the blood-brain barrier. This review explores the potential of intranasal peptide delivery for AD treatment, beginning with an overview of the disease's mechanisms and existing therapies. We discuss the challenges of targeting the brain, examine nose-to-brain delivery pathways, and highlight recent advancements in delivery techniques, including the role of nanoparticles in improving efficacy. Our goal is to encourage further research into these innovative delivery strategies that could improve patient compliance and treatment outcomes. While preclinical studies indicate substantial promise, advancing these findings into clinical applications remains crucial to overcoming drug delivery challenges and ensuring long-term safety.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214474"},"PeriodicalIF":6.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144912781","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell membrane-engineered nanoparticles: A bionic platform for targeted cancer therapy","authors":"Km Rafiya ,&nbsp;Shahzad Alam ,&nbsp;Arif Nadaf ,&nbsp;Nazeer Hasan ,&nbsp;Farhan Jalees Ahmad","doi":"10.1016/j.bioadv.2025.214471","DOIUrl":"10.1016/j.bioadv.2025.214471","url":null,"abstract":"<div><div>Cancer remains one of the most challenging diseases for targeted drug delivery despite the advancements in conventional as well as cutting-edge treatments. In recent years, rapid progress has been made in the field of nanomedicine, offering novel strategies, holding the potential to transform healthcare by enhancing bioavailability, efficacy and safety. Notably, the targeting ability of these nanotherapeutic drug delivery systems has been refined by tailoring nanoscale properties and modified surface features, furnishing an alternative approach to tumour-targeted therapy. However, current nanocarrier systems still suffer from unexpected off-target effects, immune clearance, and limited penetration into several biological barriers, including the blood-brain barrier and tumour microenvironment. To address these barriers, the biomimicking approach has come into existence, especially nanoparticles cloaked within the biological cell membranes, which mimic the natural cell function, enabling enhanced circulation half-life, more efficient interaction with the tumour microenvironment and immune evasion. Besides such vast advantages, these biomimicking nanoparticles face ongoing challenges including manufacturing scalability, potential immunogenicity and regulatory clearance for bench to bedside use. This review discusses the recent advancements and limitations of the biomimicking nanocarriers-based drug delivery systems for cancer treatment, especially focused on the cell membrane-coated nanocarriers, covering their types, fabrication methods, sources, and present challenges and their applications in cancer treatments. Overall, the current review offers a roadmap for future research in biomimicking nanoparticles and their clinical implementation.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214471"},"PeriodicalIF":6.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903134","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Novel dual responsive embelin fabricated ZnO nanomaterials amplify DNA damage and induce apoptosis via pERK1/2/p53 pathway in pancreatic ductal adenocarcinoma","authors":"Pooja S. Rajaput ,&nbsp;R. Hari Krishna ,&nbsp;K. Pradeepa ,&nbsp;P. Meghana ,&nbsp;R. Sandeep Kumar Jain ,&nbsp;N. Prashanth ,&nbsp;B.S. Ravindranath ,&nbsp;R. Sharath ,&nbsp;N.D. Satyanarayan ,&nbsp;H. Raja Naika ,&nbsp;H.M. Kumaraswamy","doi":"10.1016/j.bioadv.2025.214470","DOIUrl":"10.1016/j.bioadv.2025.214470","url":null,"abstract":"<div><div>Pancreatic ductal adenocarcinoma (PDAC) is a highly aggressive cancer with poor prognosis and chemoresistance. Nano-bioconjugates, due to their enhanced surface-to-volume ratio, offer significant potential in cancer therapy. In this study, we synthesized ZnO nanoparticles (NPs) using solution combustion method and exhibited a particle size range of 20–70 nm as confirmed by TEM analysis. These NPs were conjugated with embelin, a natural benzoquinone compound. Successful conjugation was confirmed using FTIR spectroscopy. Structural and morphological characteristics of the conjugates were confirmed using XRD, SEM, TEM, FTIR, EDS color mapping. Embelin conjugated ZnO NPs (Emb-ZnO NPs) were evaluated against PDAC cell lines (PANC-1 and MIA PaCa-2). The nanoconjugates showed significant cytotoxicity compared to individual Embelin and ZnO NPs, with IC₅₀ values of 7.05 ± 0.96 μg/ml (PANC-1) and 8.66 ± 1.46 μg/ml (MIA PaCa-2). Emb-ZnO NPs exhibited tumoricidal effects in clonogenic and migration assays. Fluorescent staining revealed disrupted cellular architecture and significant apoptosis. Immunoblot analysis exhibits deregulation of key pathways, including amplified expression of γ-H2AX (88.48 %)<em>,</em> indicative of DNA damage. Concurrently, elevated levels of pChk2 (68.07 %), p53 (89.34 %), and caspase-3 (26.67 %) promote Cell cycle halting and programmed cell death triggered by genomic instability. Conversely, reduced pERK1/2 (53.77 %) expression suggested inhibition of the MAPK pathway by Emb-ZnO NPs. Additionally, the formulation inhibited neovascularization in the CAM model, indicating anti-angiogenic potential. Molecular dynamics simulations of p53 and pERK1/2 aligned with in vitro results. In conclusion, Emb-ZnO NPs is a promising therapeutic candidate for PDAC and other cancers.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"179 ","pages":"Article 214470"},"PeriodicalIF":6.0,"publicationDate":"2025-08-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145005131","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A hybrid multi-objective optimization framework for designing superhydrophobic coatings on magnesium alloys for biomedical applications","authors":"Binod Barai ,&nbsp;Vikash Kumar ,&nbsp;Pratik Das ,&nbsp;Subhasish Sarkar ,&nbsp;Piyali Basak ,&nbsp;Buddhadeb Oraon ,&nbsp;Tapendu Mandal","doi":"10.1016/j.bioadv.2025.214469","DOIUrl":"10.1016/j.bioadv.2025.214469","url":null,"abstract":"<div><div>A novel eco-friendly coating process was developed to enhance the corrosion resistance and biocompatibility of Magnesium alloys for biomedical applications. The coating, composed of stearic acid and ZnCl₂, was optimized using a hybrid framework integrating experimental design, machine learning, and multi-objective optimization algorithms. Response Surface Methodology with Central Composite Design (RSM-CCD) was employed to systematically explore the effects of the process parameters on the surface roughness, surface free energy, and corrosion resistance efficiency. After evaluating all response data, an Artificial Neural Network (ANN) model was developed to train and predict outcomes. Following the data augmentation process, a highly accurate model was obtained, yielding an R² value exceeding 0.99. The Non-Dominated Sorting Genetic Algorithm II (NSGA-II) was coupled with an ANN to generate a diverse Pareto front, which was further refined using Teaching-Learning-Based Optimization (TLBO) and Multiobjective Particle Swarm Optimization (MOPSO). The optimized coatings exhibited a superhydrophobic surface with a water contact angle of 152° ± 1°, significantly enhanced corrosion resistance (92.4 % efficiency), and reduced corrosion rate (0.180 mm/year) compared with the uncoated substrate. Characterization techniques, including XRD, SEM, EDS, FTIR, and Raman spectroscopy, confirmed the formation of protective metal stearate compounds (Zn[CH₃(CH₂)₁₆COO]₂, Mg[CH₃(CH₂)₁₆COO]₂) and the presence of key functional groups. Live/dead cell assays demonstrated the biocompatibility of the optimized coatings, with increased cell proliferation observed after 48 h. This study presents a comprehensive, data-driven approach for developing high-performance, eco-friendly coatings for biomedical Mg alloys, offering a promising solution for biodegradable implant applications.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214469"},"PeriodicalIF":6.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906962","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Cell membrane-immobilized magnetic fluorescence nanoparticles as a screening platform for drug lead discovery","authors":"Yi Qin ,&nbsp;Xu Jiang ,&nbsp;Runuo Wang ,&nbsp;Zhi Jia ,&nbsp;Yang Liu ,&nbsp;Xun Gao ,&nbsp;Longshan Zhao ,&nbsp;Xuefeng Guan","doi":"10.1016/j.bioadv.2025.214472","DOIUrl":"10.1016/j.bioadv.2025.214472","url":null,"abstract":"<div><div>Cell membrane biomimetic screening technology enables nanomaterials to have unique biointerface targeting function and is widely used in the field of drug lead compound discovery. Here, Coronary artery smooth muscle cell (CASMC) membrane camouflaged Nile Red-doped Polyethyleneimine (PEI) functionalized magnetic fluorescent nanomaterials (CMMFNPs) were synthesized based on the covalent coupling method and were employed for the enrichment and screening of anti-coronary heart disease active ingredients targeting CASMC from the Sanwei Tanxiang capsule. Various characterization tools confirmed the CMMFNPs were successfully prepared and possessed strong biocompatibility, good optical properties, magnetic properties and adsorption properties. Eventually, a total of six potential active ingredients were screened from the extract, and the pro-proliferative effects of the screened ligands on the CASMC were preliminarily evaluated by the cellular imaging assay. In addition, molecular docking demonstrated strong binding between these compounds and cell membrane receptors (CD36, AT1); CCK-8 and BrdU kits assay further validated from different perspectives that the screened ligands promoted cell proliferation in a concentration-dependent manner. In conclusion, this method combined the cell membrane bionic screening with cellular imaging, which could realize the rapid screening of active ingredients and at the same time evaluate the pharmacological activities of the screened ligands, is expected to provide an effective tool for the discovery of new drugs.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214472"},"PeriodicalIF":6.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144906959","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Genomechanical modeling of delayed fracture healing integrating transcriptomics and tissue mechanics","authors":"Nazanin Nafisi ,&nbsp;Ahmad Hedayatzadeh Razavi ,&nbsp;Mohammad Sadegh Ghiasi ,&nbsp;Patrick Minassians ,&nbsp;Philip Hanna ,&nbsp;Aron Lechtig ,&nbsp;Kaveh Momenzadeh ,&nbsp;Abraham Mahjoob ,&nbsp;Samantha Perez ,&nbsp;Mario Keko ,&nbsp;Ramin Oftadeh ,&nbsp;Mahboubeh R. Rostami ,&nbsp;Ashkan Vaziri ,&nbsp;Rosalynn M. Nazarian ,&nbsp;Louis Gerstenfeld ,&nbsp;Marc N. Wein ,&nbsp;Fatemeh Mirzamohammadi ,&nbsp;Ara Nazarian","doi":"10.1016/j.bioadv.2025.214468","DOIUrl":"10.1016/j.bioadv.2025.214468","url":null,"abstract":"<div><div>Fracture healing is a complex biological process that involves a coordinated interplay of immune responses, gene regulation, and mechanical forces. This study integrates advanced transcriptomic (RNA sequencing) and biomechanical modeling approaches to uncover the key molecular pathways and mechanical properties that drive bone repair. Using a rat femoral delayed fracture model, researchers analyzed gene expression changes, immune cell dynamics, and tissue mechanics at different healing stages. The findings reveal critical shifts in inflammation, cartilage formation, and bone remodeling, highlighting the role of signaling pathways such as Wnt and TGF-β in regulating these transitions.</div><div>Additionally, the study introduces a genomechanical (GM) model that incorporates gene expression data into predictive biomechanical simulations. This approach allows for a more accurate prediction of tissue differentiation and mechanical strength changes over time. The study demonstrates how genetic and mechanical factors work together to optimize healing and identifies potential therapeutic targets to improve fracture recovery, especially in conditions such as diabetes, aging, and obesity, where healing is impaired.</div><div>Importantly, this work introduces an integrative modeling framework that incorporates dynamic upstream regulator activity into a mechanoregulatory framework, enabling time-resolved simulation of gene-driven tissue transitions. The GM model provides a biologically informed platform for predicting healing trajectories and identifying optimal therapeutic windows, setting the stage for future applications in personalized and condition-specific treatment planning.</div><div>By bridging molecular biology with mechanical modeling, this research provides new insights into the biological mechanisms of bone repair, paving the way for personalized treatment strategies. The GM model offers a powerful tool for predicting healing outcomes and designing targeted interventions, ultimately improving patient care in orthopaedic medicine. These findings contribute to a growing body of knowledge that seeks to enhance fracture healing through precision medicine and advanced computational modeling.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214468"},"PeriodicalIF":6.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896419","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of green nanoparticles in dental materials: A scoping review","authors":"Mohammad Amin Amiri ,&nbsp;Mahtab Memarpour ,&nbsp;Setareh Valanik ,&nbsp;Vida Hajizadeh ,&nbsp;Lobat Tayebi","doi":"10.1016/j.bioadv.2025.214475","DOIUrl":"10.1016/j.bioadv.2025.214475","url":null,"abstract":"<div><h3>Objective</h3><div>Green nanoparticles (NPs) are a key research area in dental materials. Numerous studies have investigated green NPs in dental applications because they are cost-effective and eco-friendly. This scoping review examines recent advancements in the application of green NPs in various dental materials.</div></div><div><h3>Methods</h3><div>The scoping review followed the PRISMA extension for Scoping Reviews (PRISMA-ScR) guidelines. Studies published up to April 20, 2025 were identified by a systematic search of five databases (PubMed, Scopus, Web of Science, Embase, and Ovid). Articles published in English that were peer-reviewed in vitro and in vivo studies investigating the application of green NPs in dental materials were selected. The extracted data included the types of NPs, characterization assays, dental material applications, and key performance outcomes.</div></div><div><h3>Results</h3><div>Thirty-six articles were included, 34 in vitro and 2 in vivo. The studies evaluated green NPs utilized in dental implants and adhesives, glass ionomer cement (GIC), hybrid dental composites, mineral trioxide aggregates, calcium hydroxide liners, pulp capping agents, varnishes, and tissue conditioners. The results indicated that silver (AgNPs), titanium dioxide (TiO₂NPs), and zirconium (ZrNPs) green NPs improved both antibacterial properties and mechanical performance of the assessed dental materials, and enhanced biological, mechanical and physical properties of these materials.</div></div><div><h3>Conclusion</h3><div>Although green NPs have desirable biological outcomes, the mechanical and physical outcomes depend on the type of dental material and specific characterization test. Fine-tuning their characteristics and selecting the appropriate type of NP to achieve the desired properties in dental materials is of utmost importance.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214475"},"PeriodicalIF":6.0,"publicationDate":"2025-08-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144896418","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"3D-printed scaffold with biomimetic gradient structure for promoting bone regeneration through inhibiting inflammation and facilitating in-situ biomineralization","authors":"Dongyu Wu ,&nbsp;Shangjun Gao ,&nbsp;Shaohua He ,&nbsp;Wanling Liu ,&nbsp;Qingwei Liu ,&nbsp;Siyao Lan ,&nbsp;Jiaxin Chen ,&nbsp;Fenglu Li ,&nbsp;Renjie Ruan ,&nbsp;Jin Zhang ,&nbsp;Guoming Liu","doi":"10.1016/j.bioadv.2025.214467","DOIUrl":"10.1016/j.bioadv.2025.214467","url":null,"abstract":"<div><div>Critical-sized bone defects caused by trauma, congenital malformation, or tumor resection remain a major challenge around the world. Current bone tissue-engineering scaffolds are partially confined by inadequate scaffold architecture design that mismatches with natural bone tissue, which affect normal biological functions like inflammation modulation and biomineralization, thus impairing bone regeneration process. Herein, a biomimetic 3D-printed BMGP scaffold composed of polydopamine (PDA)-polylactide (PLA) scaffold and black phosphorus (BP) nanosheets/manganese carbonyl (MnCO) nanosheets/gelatin methacryloyl hydrogel (named as BMG hydrogel) was developed for augmenting bone regeneration <em>via</em> strengthening anti-inflammatory effect and promoting <em>in-situ</em> biomineralization process. Through infilling the BMG hydrogel into the gradient-porous PDA-PLA scaffold, the obtained BMGP scaffold successfully mimicked cancellous and compact bone structure and extracellular matrix component in natural bone tissue. Upon being implanted into the critical-sized bone defect, a Fenton-like reaction between the MnCO nanosheet and endogenous hydrogen peroxide effectively induced carbon monoxide release, thereby improving anti-inflammatory response and facilitating macrophage reversed from pro-inflammatory M1 phenotype to anti-inflammatory M2 phenotype. Meanwhile, the BP nanosheet underwent degradation and <em>in-situ</em> biomineralization, which accelerated calcium phosphate formation and enhanced osteogenesis. Based on <em>in-vitro</em> and <em>in-vivo</em> data, the 3D-printed BMGP scaffold that integrated structural and functional biomimicry exhibited desirable inflammatory inhibition and <em>in-situ</em> biomineralization performances, as well as favorable osteogenic effect in rat critical-sized femoral bone defect. In all, such biomimetic scaffold obviously propelled bone regeneration process, and provided a promising strategy for treating critical-sized bone defects in clinic.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214467"},"PeriodicalIF":6.0,"publicationDate":"2025-08-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903133","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Injectable amino-modified poly-L-lactic acid microspheres/hyaluronic acid-based hydrogel composites for soft tissue fillers","authors":"Chen Shen ,&nbsp;Xingyu Zhou ,&nbsp;Junhui Jiang ,&nbsp;Junbo Dang ,&nbsp;Tong Lin ,&nbsp;Ruibo Hu ,&nbsp;Tianhao Zhao ,&nbsp;Dahui Sun ,&nbsp;Mei Zhang","doi":"10.1016/j.bioadv.2025.214465","DOIUrl":"10.1016/j.bioadv.2025.214465","url":null,"abstract":"<div><div>Soft tissue fillers have received much attention for the treatment of skin aging and the restoration of missing or excised soft volumes. However, the development of fillers with both safety and applicability remains a challenge. This study presented a synergistic composite filler comprising aldehyde-modified hyaluronic acid (OHA), hydrazide-modified hyaluronic acid (NHA), amino-modified poly-L-lactic acid microspheres (NPLLA), and antioxidant copper peptide. The hydrogel formed <em>in situ via</em> Schiff base reaction exhibited excellent mechanical properties and immediate filling effect. The amino-modified PLLA microspheres were less likely to agglomerate, avoiding the occurrence of adverse reactions and further enhancing the mechanical properties of the filler. The timed-release platform provided protection for copper peptide stability, scavenged reactive oxygen species during the fibrotic encapsulation phase, and synergistically promoted collagen deposition. In a nude mice skin aging model, hydrogels stimulated collagen production through the TGF-β/Smad signaling pathway with good long-term filler efficacy. Therefore, NPLLA microspheres/hyaluronic acid-based composite hydrogels exhibit great potential as injectable soft tissue fillers.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214465"},"PeriodicalIF":6.0,"publicationDate":"2025-08-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144903107","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of bioactive humanized collagen I-PCL composites with enhanced mechanical properties for tissue engineering applications","authors":"Shuiping Ouyang ,&nbsp;Jing Cui ,&nbsp;Xuechun Wang ,&nbsp;Chaoyue Zhang ,&nbsp;Fei Li ,&nbsp;Jia Ouyang","doi":"10.1016/j.bioadv.2025.214461","DOIUrl":"10.1016/j.bioadv.2025.214461","url":null,"abstract":"<div><div>The hierarchical assembly of collagen critically affected its biological activity, with type I collagen (COL1) being especially important for tissue organization and cell adhesion. This study aimed to identify COL1 fragments that could be biosynthesized by <em>Pichia pastoris</em> while retaining functional bioactivity. We fragmented COL1 and selected variants based on higher thermal stability (<em>T</em>_m), net charge, and predicted bioactivity. Three of the ten variants were successfully secreted by <em>P. pastoris</em> and formed triple-helix at 4 °C. For practical applications, we focused on two variants that maintained their triple-helical conformation above 15 °C, and further confirmed their higher-order structures using microscopy and rheological analysis. The variant COL109 demonstrated enhanced promotion of osteoblast differentiation but exhibited weak mechanical strength when preparing hydrogels. To address this, we enhanced its structural integrity by fusing it with an adhesion tag of (GPP)₁₀ motif. The resulting construct of COL109(GPP)₁₀ showed improved mechanical strength. Furthermore, we combined COL109(GPP)₁₀ with polycaprolactone (PCL), showing that higher PCL concentrations increased mechanical strength but reduced bioactivity and water retention, while a 1:1 ratio of COL109(GPP)₁₀ to PCL achieved an optimal balance. By promoting wound healing and cell differentiation, the composite demonstrates strong potential for use in tissue engineering.</div></div>","PeriodicalId":51111,"journal":{"name":"Materials Science & Engineering C-Materials for Biological Applications","volume":"178 ","pages":"Article 214461"},"PeriodicalIF":6.0,"publicationDate":"2025-08-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144880130","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":2,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}