Cell Biochemistry and Biophysics最新文献

{"title":"Correction: FOXM1, a super enhancer-associated gene, is related to poorer prognosis and gemcitabine resistance in pancreatic cancer.","authors":"Jian Jiang, Tianci Shen, Dan Chen, Zihao Dai, Xuelong Wang, Qiang Meng, Zhuo Yang, Di Zhang, Xiaoyi Guo, Jianqiang Xu, Jiangning Gu, Changmiao Wang","doi":"10.1007/s12013-025-01710-9","DOIUrl":"10.1007/s12013-025-01710-9","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3985"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143565547","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Disable 2, A Versatile Tissue Matrix Multifunctional Scaffold Protein with Multifaceted Signaling: Unveiling Role in Breast Cancer for Therapeutic Revolution.","authors":"Nidhi N Shah, Bhavarth P Dave, Kashvi C Shah, Disha D Shah, Kunal G Maheshwari, Mehul R Chorawala","doi":"10.1007/s12013-025-01715-4","DOIUrl":"10.1007/s12013-025-01715-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3999"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143646778","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Protective Effect of Artemisinin Against Luperox Induced Oxidative Stress and Insulin Resistance via Pi3k/Akt Pathway in Zebrafish Larvae.","authors":"Kadhirmathiyan Velumani, P Sundar Rajan, Mohammed Rafi Shaik, Shaik Althaf Hussain, Baji Shaik, Ajay Guru, Praveen Kumar Issac","doi":"10.1007/s12013-025-01747-w","DOIUrl":"10.1007/s12013-025-01747-w","url":null,"abstract":"<p><p>Oxidative stress plays a critical role in the development of insulin resistance (IR), a key factor in metabolic disorders such as diabetes. Plant active ingredients play a crucial role in protecting organisms from environmental stressors and have shown promising therapeutic potential against various metabolic disorders. Artemisinin (ART), a sesquiterpenoid with a lactone ring obtained from the herb Artemisia annua, exhibits promising therapeutic properties. This study investigates the potential of ART on Luperox (LUP)-induced oxidative stress and the resulting IR in zebrafish larvae, specifically investigating the involvement of the PI3K/AKT signaling pathway. Zebrafish larvae were chosen due to their high sensitivity to oxidative stress, well-characterized glucose metabolism, and genetic similarity to human metabolic pathways. They were exposed to LUP to induce oxidative stress, followed by treatment with ART. The effects were evaluated through biochemical assays, fluorescence staining and gene expression analysis. ART effectively restored key antioxidant enzymes (SOD, CAT, GSH) and mitigated oxidative stress evidenced by reduction in intercellular ROS and lipid peroxidation, as confirmed through DCFDA and DPPP staining assays. Additionally, ART improved glucose uptake and lowered blood glucose levels. Gene expression analysis further indicated increased levels of PI3K/Akt signalling components and antioxidant-related genes (NRF2, HO-1, GPx, and GSR). Our results indicate that artemisinin significantly alleviates oxidative stress by reducing ROS levels and enhancing antioxidant enzyme activity. Furthermore, artemisinin mitigates IR by restoring glucose metabolism and upregulating PI3K/AKT pathway components. These findings highlight the translational potential of plant active ingredients, particularly artemisinin, for the development of therapies targeting IR and oxidative stress-related metabolic disorders.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3693-3705"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143957993","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Structural and Energetic Insights into the Binding of _L- and _D-Arginine Analogs with Neuropilin-1 (NRP1): Molecular Docking, Molecular Dynamics and DFT Calculations.","authors":"Mahmoud A A Ibrahim, Dina E M Mohamed, Khlood A A Abdeljawaad, Alaa H M Abdelrahman, Shaban R M Sayed, Mohamed A El-Tayeb, Peter A Sidhom, Paul W Paré","doi":"10.1007/s12013-025-01754-x","DOIUrl":"10.1007/s12013-025-01754-x","url":null,"abstract":"<p><p>Neuropilin-1 (NRP1) is a transmembrane glycoprotein that binds numerous ligands, including vascular endothelial growth factor A (VEGFA) that stimulates blood vessel formation. Preclinical trials propose that NRP1 inhibition blocks neoplasm cell proliferation and slows tumor growth by suppressing angiogenesis. As such, VEGFA/NRP1 signaling is a potential target for carcinoma inhibition. Since arginine (Arg) regulates nutrient-responsive rapamycin signaling, which in turn regulates cell growth and metabolism, Arg, as well as simple structural variations of _L- and _D-Arg, were selected to study in-silico structural and energetic influences of such ligands on NRP1 signaling. Initially, AutoDock Vina1.1.2 software performance was assessed to predict binding modes of Arg analogs with NRP1 based on the available experimental data. Molecular docking and molecular dynamics (MD) simulations over 100 ns were run to inspect the potency of Arg analogs to bind with NRP1. Analog-NRP1 complex binding affinities (ΔG_binding) were evaluated using the MM/GBSA approach. Results indicated that _L-/_D-Agd- and _L-/_D-Agn-NRP1 complexes exhibited binding affinities greater than the co-crystallized _L-homoarginine ligand (calc.-31.2 kcal.mol^-1) with ΔG_binding values of -40.5/-40.6 and -40.0/-36.2 kcal.mol^-1, respectively. Structural and energetic analyses were performed to examine further _L-/_D-Agd and _L-/_D-Agn. Quantum mechanical calculations were performed to confirm the outcomes obtained from docking computations and MD simulations.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3787-3801"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143960409","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Turmeric on Manganese-induced Redox Imbalance in Testicular Tissues: Histo-micrometric and Biochemical analyses.","authors":"Fatima Bashir Ahmad, Chaman Ara, Shaukat Ali, Shagufta Andleeb, Hafiz Abdullah Shakir, Faheem Nawaz, Asmatullah","doi":"10.1007/s12013-025-01763-w","DOIUrl":"10.1007/s12013-025-01763-w","url":null,"abstract":"<p><p>Manganese is the most overlooked chemical element, though it is the 5^th most abundant metal in earth' crust. Despite being important in the body's mechanisms, bioaccumulation of manganese in living organisms can be noxious. The current study aimed to evaluate the effects of manganese on male mice's reproductive parameters, and turmeric was used as a remedial agent against manganese-induced potential subchronic toxicity. Healthy male mice were distributed into seven groups (n = 10), Control (untreated), Vehicle control; VC-I (0.1 ml of saline solution), Mn-I (5 mg/Kg BW), Mn-II (10 mg/Kg BW), Mn-III (20 mg/Kg BW), Mn + T (20 mg of Mn/Kg +100 mg turmeric/Kg BW) and Vehicle control; VC-II (0.1 ml of olive oil) via oral gavage routinely, once a day. The experiment was continued for 28 days. Before administrations, the antioxidant capacity of turmeric was evaluated via FRAP, TPC & GC-MS assays. Mice were acclimatized for 10 days after dosing, then euthanized and samples (testis & blood) were recovered. Morphological observations showed minute morphological changes in testes as compared to controls. Morphometric analysis revealed average body weight, testis weight and size of Mn-intoxicated mice were reduced remarkably (P ≤ 0.05) in comparison with the control group. Sideways Mn + T group showed non-significant changes in both parameters. Hematological, micrometric findings and serum testosterone, luteinizing hormone and follicle stimulating hormone were significantly different (P ≤ 0.05) in Mn-exposed groups against controls. These alterations were concomitant with histological variations in Mn-treated groups. While such deviations were less obvious in the Mn + T-administered group. The aforesaid findings deduced that the turmeric supplementation manifested improvements in most of the histo-micrometric, hematological, steroidal parameters and enzymatic indices of mice through its antioxidant action.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3889-3903"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Determination of the Inhibitory Potential of Chalcones on Myeloperoxidase Enzyme Activity: In vitro and Molecular Docking Studies.","authors":"Nurgül Abul, Yeliz Demir, Aykut Öztekin, Hasan Özdemir","doi":"10.1007/s12013-025-01719-0","DOIUrl":"10.1007/s12013-025-01719-0","url":null,"abstract":"<p><p>Myeloperoxidase (MPO) is a highly abundant hemoprotein in neutrophils and monocytes. It has a crucial function in immunological surveillance and the body's defensive systems. Nevertheless, there is a strong correlation between elevated MPO activity and the development and advancement of inflammatory processes. Chalcone derivatives serve as fundamental components of pharmaceutical raw materials, which have been extensively utilized for the treatment of several ailments. In this study, it was studied the effect of some chalchones on MPO activity. Chalcones (1-6) strongly inhibited MPO with IC_50s in the micromolar range of 0.05-0.828 µM. In particular, 4,4'-difluorochalcone (3) exhibited the best MPO inhibitory impact with IC₅₀ of 0.05 µM. Additionally, molecular docking experiments were conducted to predict the binding affinities and interactions of the chalcone derivatives with the MPO active site. The docking results revealed that all tested compounds exhibited favorable binding energies, with ΔG Vina values ranging from -7.6 to -8.4 kcal/mol. Compound 3 demonstrated the strongest binding affinity (-8.4 kcal/mol), forming key hydrogen bonds with Gln91 and His95, and halogen interactions with the fluorine atoms, which may account for its enhanced inhibitory activity. These combined in vitro and in silico results suggest that chalcone derivatives hold significant potential as therapeutic candidates targeting MPO.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3383-3393"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143623070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"RNA Binding Protein IGF2BP3 Rehabilitates Glycolysis of Vascular Endothelial Cells to Protect the ox-LDL-induced Cellular Injury via Stabilizing LDHA mRNA.","authors":"Xing-Chun Mo, Ping Lu, Qu-Cheng Wei, Xiao-Jing Yang","doi":"10.1007/s12013-025-01735-0","DOIUrl":"10.1007/s12013-025-01735-0","url":null,"abstract":"<p><p>Vascular endothelial cells (VECs) dysfunction has been revealed to be a major cause of various cardiovascular diseases. Yet, the precise cellular and molecular mechanisms of VECs injury remain elusive. This study aims to investigate the roles and molecular mechanisms of RNA binding protein IGF2BP3 in vascular endothelial cell injury caused by oxidized low-density lipoprotein (ox-LDL). HUVECs were treated with ox-LDL to induce endothelial cell injury, and the cellular responses to ox-LDL were assessed using cell viability and apoptosis assays. IGF2BP3 was expressed at low levels in vascular tissues from atherosclerosis patients. Treatment with ox-LDL significantly decreased the expression of IGF2BP3 in HUVECs. Overexpression of IGF2BP3 effectively reduced the injury induced by ox-LDL. Glucose metabolism enzymes were significantly downregulated in vascular tissues from atherosclerosis patients and in HUVECs treated with ox-LDL, leading to suppressed glucose metabolism. IGF2BP3 upregulated the glucose metabolism enzyme LDHA to alleviate the injury caused by ox-LDL in HUVECs. Analysis of the LDHA sequence revealed the presence of an IGF2BP3 binding motif in its 3'UTR. Further experiments including RNA pull-down, RNA IP, and RNA stability assays confirmed the specific binding of IGF2BP3 to the 3'UTR region of LDHA, stabilizing its transcripts. Rescue experiments demonstrated that IGF2BP3 mitigated vascular endothelial cell injury by regulating LDHA-mediated glucose metabolism. The outcomes of this study elucidate the protective roles of IGF2BP3 in safeguarding vascular endothelial cells against injury.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3547-3555"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143750596","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"\"SERBP1 (Hero45) is a Novel Link with Ischemic Heart Disease Risk: Associations with Coronary Arteries Occlusion, Blood Coagulation and Lipid Profile\".","authors":"Vladislav Shilenok, Ksenia Kobzeva, Olga Bushueva","doi":"10.1007/s12013-025-01736-z","DOIUrl":"10.1007/s12013-025-01736-z","url":null,"abstract":"<p><p>Ischemic heart disease (IHD), stemming from coronary atherosclerosis, involves pathological processes in which chaperone proteins play an essential role. SERBP1 (Hero45), an RNA-binding protein, has recently been ascribed to the newly discovered class of Hero proteins with chaperone-like activity, making it particularly relevant in atherosclerosis-related diseases. In this study, 2164 subjects (836 IHD patients and 1328 controls) were genotyped for five common single nucleotide polymorphisms (SNPs) of SERBP1 using probe-based PCR. Here, we report that SNPs of SERBP1 are associated with reduced risk of left coronary artery atherosclerosis: rs4655707 (effect allele [EA] T, OR = 0.63, 95% CI 0.43-0.93, p = 0.02), (EA C, OR = 0.63, 95% CI 0.42-0.95, p = 0.02), rs12561767 (EA G, OR = 0.65, 95% CI 0.45-0.96, p = 0.03), rs6702742 (EA A, OR = 0.63, 95% CI 0.43-0.94, p = 0.02). Additionally, SERBP1 loci are linked to lower coronary artery stenosis (rs1058074), improved blood lipid profiles (rs1058074), and favorable blood coagulation parameters (rs4655707, rs6702742, rs1058074, rs12561767). Together, our study is the first to provide evidence that SERBP1 is involved in lipid metabolism and coagulation regulation, modulating IHD risk.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3557-3569"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143770839","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Development of Novel α-Amylase Inhibitors: Synthesis, Molecular Docking, and Biochemical Studies.","authors":"K Ramakrishnan, Reshma Rajan, Lenin Nachimuthu, Premkumar Jayaraj, Chandrakala A Narasimhulu, Pragney Deme, Sanjay Rajagopalan, Akella Sivaramakrishna, S Karthikeyan, Rajagopal Desikan","doi":"10.1007/s12013-025-01759-6","DOIUrl":"10.1007/s12013-025-01759-6","url":null,"abstract":"<p><p>The rising prevalence of diabetes as a major non-communicable disease underscores the critical need for effective anti-diabetic agents. The new analogs designed 3a-3j were effectively synthesised and thoroughly characterised using (¹H, ¹³C NMR, FT-IR, GCMS, and HRMS) to investigate their structural biochemical properties. The novel analogs were investigated thoroughly by in silico (molecular docking) and in vitro (anti-oxidant (DPPH, ABTS) activity, anti-inflammation (RBC), modifications of LDL and HDL, thiobarbituric substances, cholesterol efflux assay, and anti-diabetic) assays, validated for α-amylase inhibition. Enzyme inhibition results showed α-amylase IC₅₀ values of 1.79 ± 0.12 μg for compound 3d, 1.75 ± 0.05 μg for compound 3e, and 1.53 ± 0.20 μg for the standard drug acarbose. Among the new molecules, compounds 3c and 3d exhibited the highest inhibitory activity in all performed in silico and in vitro studies. The study demonstrated that inhibitors 3a-3j bind strongly to the active site of human pancreatic α-amylase, highlighting their potential as effective inhibitors. These research findings help to improve the field of developing lead molecules for anti-diabetic agents.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3859-3876"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955452","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Macrophage Polarization-Based Analysis of the Role of the FOXM1/KIF20A Axis in Breast Cancer Metastasis.","authors":"Yuanbin Wang, Ruimin Ma, Qing Yang, Lijun Yang, Xiangli Li, Zhihao Wu","doi":"10.1007/s12013-025-01755-w","DOIUrl":"10.1007/s12013-025-01755-w","url":null,"abstract":"<p><p>To investigate the potential molecular processes underlying the function of forkhead box M1 (FOXM1)-mediated macrophage polarization in breast cancer (BC). The expression levels of Kinesin family member 20 A (KIF20A) and FOXM1 in BC tissues and tumor-associated macrophages (TAMs) were determined using RT-qPCR. Following co-culture of macrophages with BC cells, the impact of FOXM1 on the proliferation, invasive migration ability, and epithelial-mesenchymal transition (EMT) of BC cells was assessed using cell counting kit-8, Transwell, and Western blot assays respectively. Both the chromatin immunoprecipitation (ChIP) test and the dual luciferase reporter gene assay were used to confirm the connection between FOXM1 and KIF20A. Furthermore, the effect of FOXM1 on BC cell growth in vivo was evaluated via subcutaneous tumor formation assay conducted in nude mice. BC cell growth and metastasis were aided by M2 macrophage polarization. KIF20A and FOXM1 expression levels were markedly elevated in both TAMs and BC tissues. FOXM1 drived M2 polarization of macrophages by transcriptionally activating KIF20A. In vitro studies have demonstrated that FOXM1, through its regulation of KIF20A, enhanced BC cell proliferation, migration, invasion, and EMT. The upregulation of KIF20A expression by FOXM1 promotes M2 polarization of macrophages, thereby facilitating BC cell proliferation and metastasis.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3803-3813"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143952237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}