Cell Biochemistry and Biophysics最新文献

{"title":"FOXO1 Promotes Mitophagy in Granulosa Cells by Targeting the METTL3/SMAD4 axis.","authors":"Lan Luo, Mi Tang, Yanli Peng, Xi Chen, Yaqi Liu, Jialan Cui","doi":"10.1007/s12013-026-02143-8","DOIUrl":"10.1007/s12013-026-02143-8","url":null,"abstract":"<p><p>Diminished ovarian reserve decreases the chance of fertility, but the mechanisms regulating ovarian function remain unclear. This study aimed to explore the mechanism through which FOXO1 regulates mitochondrial function in ovarian granulosa cells. KGN cells were transfected with FOXO1, METTL3, and/or SMAD4 vectors, and then their proliferation and apoptosis were detected using CCK-8 and EdU assays and flow cytometry, respectively. Molecular binding was detected by immunoprecipitation. The effect of FOXO1 on METTL3 promoter activity was analyzed using luciferase reporters. SMAD4 m⁶A was quantified using MeRIP-qPCR. ROS and MMP were measured using immunofluorescence, ATP and mtDNA levels were determined using their respective kits, and the expression of mitophagy-related proteins, including DRP1, PINK1, and parkin, was quantified using Western blotting. FOXO1 overexpression or SMAD4 knockdown reduced the proliferation of KGN cells and enhanced their apoptosis. FOXO1 inhibited METTL3 transcription via binding. METTL3 promoted SMAD4 m⁶A modification. FOXO1 repressed METTL3 and SMAD4 expression, while METTL3 upregulated SMAD4 expression. In FOXO1-overexpressing cells, METTL3 or SMAD4 overexpression enhanced proliferation, suppressed apoptosis, reduced ROS production, elevated ATP, mtDNA, and MMP levels, and downregulated DRP1, PINK1, and parkin expression. In conclusion, FOXO1 targeted METTL3 to downregulate SMAD4 and modulated the expression of mitophagy-related proteins, thereby promoting mitophagy and mitochondrial damage in granulosa KGN cells.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4523-4533"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758276","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Elucidation of Baicalein's Anti-Intervertebral Disc Degeneration Mechanism Through Network Pharmacology and Experimental Validation.","authors":"Rui Wang, Zhiyong Liu, Xiaopeng Zhao","doi":"10.1007/s12013-026-02146-5","DOIUrl":"10.1007/s12013-026-02146-5","url":null,"abstract":"<p><p>This study aims to elucidate the biological effects of Baicalein (BA) on intervertebral disc degeneration (IDD) and the potential mechanisms involved. Network pharmacology analysis was performed to identify the core targets and signaling pathways responsible for the therapeutic effects of BA on IDD. In vitro validation was carried out using IL-1β-stimulated nucleus pulposus cells (NPCs). Cell viability, apoptosis, inflammatory cytokines (TNF-α, IL-6), extracellular matrix (ECM)-related molecules (MMP3, Aggrecan, Collagen II), and the phosphorylation of ERK and p38 were measured using CCK-8 assay, flow cytometry, qRT-PCR, ELISA and Western blot. Network pharmacology identified 80 common targets and highlighted the MAPK signaling pathway as a key mechanism. In vitro, BA (50 µmol/L) mitigated IL-1β-induced cytotoxicity in NPCs and promoted cell proliferation. BA significantly inhibited apoptosis by regulating the Bcl-2/Bax family and cleaved c-caspase-3. It also decreased the expression of TNF-α and IL-6, and reversed ECM homeostasis by modulating MMP-3, Aggrecan and Collagen II expression. Furthermore, BA treatment effectively suppressed IL-1β-induced phosphorylation of ERK and p38. Notably, the MAPK agonist anisomycin partially abolished the above protective phenotypes of BA and restored MAPK phosphorylation. BA alleviates apoptosis, inflammatory response and ECM degradation in NPCs, to retard IDD development mainly through inhibiting MAPK pathway activation, supporting the clinical translational potential of BA for IDD treatment.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4571-4581"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585776/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811850","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Probing Voltage Dependence Interaction of Cationic Peptides with Bacterial Porins at A Single-Molecule Level.","authors":"Sonal Prasad","doi":"10.1007/s12013-026-02148-3","DOIUrl":"10.1007/s12013-026-02148-3","url":null,"abstract":"<p><p>This study examined how cationic α‑helical antimicrobial peptides such as protamine exchange between the aqueous phase and the transmembrane β‑barrel porin OmpF (E. coli) at the single‑molecule level. High‑resolution conductance recordings in planar lipid bilayers revealed clear voltage‑ and concentration‑dependent ion current blockages, consistent with transient peptide interactions within the OmpF lumen. Peptide length influenced the characteristics of these interactions: longer protamine fragments produced weaker and less frequent blockages. At low transmembrane potentials, all peptides interacted weakly with the pore, whereas higher potentials increased the likelihood and duration of detectable events. These findings establish OmpF as a tractable system for probing cationic peptide-channel interactions and provide a descriptive framework for understanding how arginine‑rich peptides engage with β‑barrel pores due to likely electrostatic trapping, without implying specific transport pathways or translocation mechanisms.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4583-4597"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585899/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758788","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Three-Dimensional Hyaluronic Acid-Based Scaffold Model for Electroporation-Enhanced Resveratrol Therapy in Triple-Negative Breast Cancer.","authors":"Pragatheiswar Giri, Monica Dettin, Annj Zamuner, Maria Teresa Conconi, Ignacio G Camarillo, Nicolò Martinelli, Luigi Dall'Olmo, Elisabetta Sieni, Raji Sundararajan","doi":"10.1007/s12013-026-02127-8","DOIUrl":"10.1007/s12013-026-02127-8","url":null,"abstract":"<p><p>Triple-negative breast cancer (TNBC) is an aggressive malignancy defined by the absence of estrogen receptor (ER), progesterone receptor (PR), and human epidermal growth factor receptor 2 (HER2). These molecular characteristics render targeted therapies less effective and contribute to poor clinical outcomes. Towards this, we studied the electroporation-mediated delivery of resveratrol, a polyphenolic compound with pleiotropic anticancer activities, on MDA-MB-231, human TNBC cell line. Both conventional two dimensional (2D) monolayer cell culture and three-dimensional (3D) hyaluronic acid HA-EAbuK-IKVAV scaffold-based cell cultures were used. Two electroporation (EP) protocols (EP1: 8 pulses, 800 V/cm, 100 µs at 1 Hz; EP2: 8 pulses, 1000 V/cm, 100 µs at 1 Hz) were evaluated for their effects on cytotoxicity and reactive oxygen species (ROS) generation. In 3D, the combined treatment similarly reduced viability to 20% (EP1 + Resv) and 17% (EP2 + Resv), whereas in 2D, the viability reduction is 24% and 18%, respectively. Mechanistically, ROS levels increased substantially with the combination treatment: in 2D, ROS rose to 3 times (EP1 + Resv) and around 3.5 times (EP2 + Resv) the control, while in 3D, ROS increased to 2.4 times (EP1 + Resv) and 2.9 times (EP2 + Resv). Moreover, the combined treatment of electroporation and resveratrol administration on 3D HA-EAbuK-IKVAV-cultured cells decreased the expression of cell cycle regulatory protein Cdk2 and proliferation marker Ki67. These findings highlight the importance of 3D microenvironmental cues in modulating TNBC responsiveness to resveratrol and underscore the potential of combining electroporation with natural compounds as a rational therapeutic strategy for this challenging subtype of breast cancer.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4369-4380"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586016/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148766126","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Metabolomic Analysis of Intracellular Amino Acid Profile Differences Between Cisplatin-Resistant and Cisplatin- Sensitive CALU-1 Lung Cancer Cells.","authors":"Ismail Koyuncu, Mehmet Ali Tapsık, Ozgur Yuksekdag, Murat Tiken, Ebru Temiz, Hale Güler Kara","doi":"10.1007/s12013-026-02134-9","DOIUrl":"10.1007/s12013-026-02134-9","url":null,"abstract":"<p><p>Lung cancer remains one of the leading causes of cancer-related mortality worldwide, and resistance to cisplatin remains a major limitation in the treatment of non-small cell lung cancer (NSCLC). Accumulating evidence indicates that cancer cells reprogram amino acid metabolism to support proliferation, stress adaptation, redox balance, and therapeutic resistance. In this context, defining the amino acid-related metabolic alterations associated with cisplatin resistance may help identify potential metabolic vulnerabilities and biomarker candidates. In this study, intracellular free amino acid profiles were compared between cisplatin-sensitive parental human lung squamous cell carcinoma cells (CALU-1) and their cisplatin-resistant counterpart (cr-CALU-1) using liquid chromatography-tandem mass spectrometry (LC-MS/MS). The analysis revealed marked differences in the intracellular amino acid profiles of cr-CALU-1 cells compared with parental CALU-1 cells. Several amino acids, including proline, glutamine, glutamate, arginine, and alanine, were detected at higher levels in parental CALU-1 cells, whereas their intracellular levels were significantly lower in cr-CALU-1 cells. In contrast, cystine and phosphoethanolamine were increased in resistant cells. Multivariate and pathway analyses indicated that arginine and proline metabolism, histidine metabolism, and glutathione metabolism were among the most prominently affected pathways. These findings suggest that cisplatin resistance in CALU-1 cells is associated with broad remodeling of intracellular amino acid homeostasis. However, because targeted LC-MS/MS provides static metabolite pool-size information, further isotope-tracing and functional validation studies are required to determine whether these alterations reflect changes in amino acid uptake, biosynthesis, catabolism, or downstream utilization.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4423-4434"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719878","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Comparative Efficacy of Coriander Seed Extract-Loaded Selenium Nanoparticles and DMSA in Restoring Antioxidant/Apoptotic Balance of Lead-Induced Hepatotoxicity.","authors":"Eslam Elshaier, Abdel Ghany F Shoair, Rehab Reda Hassan, Mansour Emad, Noureen ELKattan, Ahmed Khalifa, Mohamed Essam, M M Farag, Fayez Althobaiti, Marwa M M Fawzy, Heba A Sahyon","doi":"10.1007/s12013-026-02144-7","DOIUrl":"10.1007/s12013-026-02144-7","url":null,"abstract":"<p><p>Lead (Pb) is a heavy metal that causes hepatotoxicity even in trace amounts. This study investigated the therapeutic potential of coriander extract-loaded selenium nanoparticles (coriander@Se-NPs) against Pb toxicity in a mouse model, comparing its efficacy to the benchmark standard chelator, DMSA. The effectiveness of coriander extract, coriander@Se-NPs, and synthetic chelator (DMSA) was evaluated. The study groups contain: a control group receiving distilled water, a Pb toxicity group given Pb-acetate at 150 mg/kg, a positive control group treated with DMSA after Pb exposure, and two groups receiving coriander extract or coriander@Se-NPs after Pb exposure, both at 200 mg/kg daily for three weeks. Our results demonstrated that the coriander@Se-NPs significantly reduced hepatic Pb concentrations (83.90%) and restored antioxidant enzyme activities (SOD and CAT) more effectively than DMSA. Notably, coriander@Se-NPs exhibited potent anti-inflammatory and anti-apoptotic effects, evidenced by a 12.5-fold reduction in IL-6 and the suppression of Caspase 3 to sub-normal levels (0.399-fold). Furthermore, the coriander@Se-NPs treatment stabilized Beclin 1 levels, suggesting a restoration of cellular autophagy homeostasis. These findings indicate that coriander@Se-NPs provide a superior, multi-modal protective shield against heavy metal poisoning compared to traditional synthetic chelators, DMSA, highlighting their potential as a high-efficacy nanomedicine for clinical toxicology. Further studies are needed to explore its potential clinical applications and mechanisms.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4535-4553"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811845","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Fabrication and Characterization of Copper Oxide Nanoparticles Using C. Gigantea Leaf Extract and Their ROS-Mediated Anticancer Activity Against A549 Lung Cancer Cells.","authors":"Bavya Swaminathan, Sivakumar Ramalingam, Renuka Saravanan","doi":"10.1007/s12013-026-02110-3","DOIUrl":"10.1007/s12013-026-02110-3","url":null,"abstract":"<p><p>The pharmacological properties of C. gigantea have been scientifically validated, making it evident the significance of this plant in both traditional and contemporary medicines. In the current investigation, CuO-NPs were synthesized using the green synthesis method with the leaf extract of C. gigantea and then its antineoplastic activities were determined against human lung carcinoma cells. The phytochemical analysis of the leaf extract of C. gigantea reveals the presence of several active chemicals, which include alkaloids, flavonoids, phenolics, saponins, terpenoids, and tannins. The antioxidant activity of the sample was analyzed using two standard methods, namely DPPH and FRAP assays. It is well documented that the sample exhibits remarkable free radicals scavenging activity. The characterization of the synthesized CuO-NPs was carried out based on its physicochemical properties. MTT assay was performed in order to study the anti-cancer activity of CuO-NPs. The outcome of the experiment indicated that there was a considerable decrease in viability of A549 cells. The cytotoxic mechanism of nanoparticles was examined using various methods, including DAPI staining, Rhodamine 123, and flow cytometry. It was observed that the cells experienced nuclear condensation, disruption of mitochondrial membrane potential, and induction of apoptosis. The main purpose of this research is to examine the use of a sustainable method for the synthesis of copper oxide nanoparticles (CuO-NPs) from C. gigantea leaves and to evaluate their physicochemical, antioxidant properties, and anticancer effects through ROS-induced mechanism against A549 human lung cancer cells.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4161-4183"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Adsorption-driven Graphene Oxide SPR Biointerfaces: Coupled Thermodynamic, Kinetic, and Optical Modeling with Effective-layer Validation.","authors":"Sadok Kouz","doi":"10.1007/s12013-026-02105-0","DOIUrl":"10.1007/s12013-026-02105-0","url":null,"abstract":"<p><p>This work presents a theoretical modeling framework for adsorption-driven graphene oxide (GO)-functionalized surface plasmon resonance (SPR) biointerfaces. Rather than describing a specific capture-probe biosensor, the model focuses on the effective optical transduction of molecular accumulation at a GO-modified interface. The framework couples transfer-matrix optical modeling of a prism/TiW/Au/GO multilayer structure with a generalized Langmuir-Hill adsorption formalism, allowing analyte concentration, surface coverage, effective interfacial refractive index, and plasmonic resonance shift to be linked within a unified description. The optical response is evaluated at 633 nm under angular interrogation. Adsorption is represented through effective surface coverage, an optically equivalent biolayer thickness, and a concentration-dependent refractive-index perturbation. Temperature dependence is incorporated using a van't Hoff-type treatment for enthalpy-driven adsorption, while time-dependent behavior is described using first-order association-dissociation kinetics as an ideal reversible limiting case. Three representative adsorption regimes are considered, corresponding to weak, moderate, and cooperative surface accumulation, with equivalent dissociation constants ranging from approximately 1.67 to 200 μM. A formal optical optimization identifies Au, TiW, and GO effective thicknesses of 49 nm, 0.20 nm, and 0.20 nm, respectively, producing a deep SPR minimum and a narrow angular linewidth. Because the TiW and GO optima occur at the lower boundary of the optical-thickness scan, they are interpreted as effective interfacial parameters rather than literal physical monolayer thicknesses. Additional simulations using physically realistic GO thicknesses of 0.7-1.5 nm show that the adsorption-driven response is preserved, with low-concentration sensitivities remaining close to 0.037-0.038 deg μM^-1. For the moderate adsorption regime, the model predicts a resonance shift of approximately 0.79^∘ over 0-100 μM, an effective biolayer thickness up to 2.72 nm, and a refractive-index sensitivity of 115.96 deg RIU^-1. The estimated detection limit is shown to depend directly on the assumed angular noise. For an angular resolution of 10^-3 degree, the model-estimated limit of detection is 0.027μM, while varying the angular noise from 10^-4 to 10^-2 degree gives limits from 0.0027 to 0.270μM. The temperature response is consistent with an exothermic adsorption case with ΔH ≈ - 26 kJ mol^-1, and the kinetic analysis provides characteristic association and dissociation times under the assumed single-rate model. The results establish a mechanism-aware computational framework for connecting adsorption thermodynamics, kinetic parameters, and SPR optical response at GO biointerfaces, while clearly identifying the effective-parameter and experimental-validation limits of the model.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4071-4088"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13585792/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315118","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Natural Antioxidant Activity Evaluation Using Electrochemically Generated Superoxide Radical Anion: The SRAGE Assay.","authors":"Fatah Keffous, Abdallah Habab, Mohamed Abbaci, Abdelkrim Cheriti","doi":"10.1007/s12013-026-02130-z","DOIUrl":"10.1007/s12013-026-02130-z","url":null,"abstract":"<p><p>The antioxidant concentration and the redox potential of the oxidizing system employed in antioxidant assays are critical parameters governing the classification of antioxidant reducing power. In this study, we introduce the Superoxide Radical Anion Generated Electrochemically (SRAGE) assay, a novel electrochemical method for evaluating the antioxidant activity of natural compounds. Superoxide is the primary reactive oxygen species generated during the one-electron reduction of molecular oxygen in mitochondria, making it a biologically relevant probe for antioxidant evaluation and a distinct advantage over conventional 2,2-diphenyl-1-picrylhydrazyl (DPPH) and 2,2'-azinobis-(3-ethylbenzothiazoline-6-sulfonic acid) (ABTS) discoloration methods.In the SRAGE assay, [Formula: see text]is generated in situ by electrochemical reduction and subsequently scavenged by natural antioxidants, including rutin, ascorbic acid, quercetin, and gallic acid. The controlled generation of superoxide was achieved using cyclic voltammetry and was validated experimentally. At a concentration of 1mmol L- 1, the tested antioxidants exhibited inhibitory activities of 44.30 ± 1.31%, 52.01 ± 0.95%, 78.73 ± 2.02%, and 100.0 ± 0.12%, respectively. The ranking of antioxidant reducing power obtained from the SRAGE assay is in close agreement with that derived from the DPPH method. The proposed SRAGE assay offers a versatile electrochemical platform for evaluating the antioxidant activity of pure compounds and complex systems, including synthetic formulations, biological extracts, pharmaceutical products, and food additives.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4409-4421"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719552","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Predicting of Nano Cerium/Hydroxyapatite Efficiency Against γ- Irradiation Induced Heart Failure in Albino Rats.","authors":"Emad M El-Sherbiny, Mohamad T Abdelrahman, Eman I Gheriany","doi":"10.1007/s12013-026-02101-4","DOIUrl":"10.1007/s12013-026-02101-4","url":null,"abstract":"<p><p>The study was conducted to investigate the therapeutic potential of nano cerium hydroxyapatite (nCe/HAp) on γ-irradiation (IR) induced cardiac failure in male rats. Cardiac failure was induced by exposure to 9 Gy of γ-radiation. The nanocomposite nCe/HAp was administered at a dose of 50 mg/kg b.w. intravenously. The levels of oxidative status, heart enzymes, troponin I (cTnI), total creatine kinase (CK), creatine phosphokinase isoenzyme (CK-MB), and lactate dehydrogenase (LDH) were measured, in addition to immune status, by measuring the levels of nuclear factor kappa B (NF-kB), tumor necrosis factor-α (TNF-α), interleukin-6 (IL-6) and C-reactive protein (CRP). Pathological analysis of heart tissue was undertaken for all animals. The results revealed the effectiveness of nCe/HAp in ameliorating most of the examined parameters by significantly elevating the GSH and SOD activity levels. On the other side, there was a significant decline in the serum NO, MDA, cTnI, NF-kB, CK, CK-MB and LDH levels upon treatment. Microscopic evaluation of tissue samples confirmed the biochemical outcomes and the variance between the control and the other treated groups. In conclusion, nCe/HAp succeeded in preventing heart damage to a considerable degree, and paves the way for Ce/HAp nanocomposites as promising effective candidates for mitigating radiation treatments.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4015-4024"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704859","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}