Cell Biochemistry and Biophysics最新文献_第6页

One-carbon Metabolism and Epigenetic Elements are Modulated by miR-1914-5p in an in Vitro Model of Steatosis. 在体外脂肪变性模型中，miR-1914-5p调控单碳代谢和表观遗传元件

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-05 DOI: 10.1007/s12013-025-01881-5

Camila Cristiane Pansa, Ana Caroline Pimentel de Oliveira, Karen C M Moraes

引用次数: 0

A New Direction for Delaying Intervertebral Disc Degeneration: Resveratrol Inhibits Ferroptosis in Nucleus Pulposus Cells. 延缓椎间盘退变的新方向：白藜芦醇抑制髓核细胞的铁下垂。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-02 DOI: 10.1007/s12013-025-01874-4

Hong Wang, Feilong Li, Yongliang Mei, Taotao Wu, Liquan Wang, Pandeng Hao, Shenjing Ci, Zongchao Liu

引用次数: 0

Commemorating Dr. Sampath (Sam) Parthasarathy for his Exceptional Research in Biochemistry and Lipidology in Health and Diseaseand Legacy as a Superb Mentor and Scientist. 纪念Sampath (Sam) Parthasarathy博士在健康和疾病的生物化学和脂质学方面的杰出研究，以及作为卓越导师和科学家的遗产。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-02 DOI: 10.1007/s12013-025-01879-z

Narasimham L Parinandi

引用次数: 0

T-Cell Receptor Excision Circle/Kappa-Deleting Recombination Excision Circle-Based Newborn Screening Program for Severe Combined Immunodeficiency in Kumamoto, Japan. 日本熊本基于t细胞受体切除圈/ kappa删除重组切除圈的新生儿严重联合免疫缺陷筛查项目。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-02 DOI: 10.1007/s12013-025-01873-5

Yuya Kinoshita, Jun Kido, Takaaki Sawada, Keishin Sugawara, Fumiko Nozaki, Tomoyuki Mizukami, Madoka Nishimura, Shinichiro Yoshida, Ryutaro Tsuru, Kimitoshi Nakamura

引用次数: 0

The Effects and Mechanisms of the YY1/EGFR Axis on Inflammation and Oxidative Stress in Asthma. YY1/EGFR轴在哮喘炎症和氧化应激中的作用及其机制

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 Epub Date: 2025-04-04 DOI: 10.1007/s12013-025-01737-y

Yue Li, Pengfei Li, Tianci Jiang, Ruhao Wu, Yu Wang, Zhe Cheng

{"title":"The Effects and Mechanisms of the YY1/EGFR Axis on Inflammation and Oxidative Stress in Asthma.","authors":"Yue Li, Pengfei Li, Tianci Jiang, Ruhao Wu, Yu Wang, Zhe Cheng","doi":"10.1007/s12013-025-01737-y","DOIUrl":"10.1007/s12013-025-01737-y","url":null,"abstract":"To investigate the expression of the transcription factor Yin and Yang 1 (YY1) and the effect of oxidative stress on the upregulation of epidermal growth factor receptor (EGFR) expression in a bronchial epithelial cell line. A bronchial epithelial cell line (BEAS-2B) was stimulated with interleukins (IL-4, IL-13, and IL-17A) to simulate the asthma microenvironment. Total RNA and total protein were extracted from the BEAS-2B cells, and the expression of inflammatory markers (TSLP and IL-25) and YY1/EGFR was determined. EGFR was overexpressed/inhibited in BEAS-2B cells, and changes in cell activity (CCK-8), thioredoxin reductase (TrxR), and malonaldehyde (MDA) expression were identified. EGFR expression was determined after interference with YY1. The binding sites of the YY1 and EGFR promoter regions were predicted by online databases. Precipitation of YY1 in the EGFR promoter region was investigated via a chromatin immunoprecipitation (ChIP) assay. After IL-4, IL-13 and IL-17A were used to stimulate BEAS-2B cells, the expression levels of inflammatory markers (TSLP and IL-25) and EGFR were significantly increased in the asthma cell model. After EGFR was overexpressed, EGFR mRNA and protein expression levels were significantly increased, cell viability was significantly increased, the relative expression level of MDA was significantly increased, and the relative expression level of TrxR was significantly decreased. After the expression of EGFR was disrupted, the EGFR mRNA and protein expression levels were significantly decreased, the cell viability was significantly decreased, the relative expression level of MDA was significantly decreased, and the relative expression level of TrxR was significantly increased. After the expression of YY1 was disrupted, the EGFR mRNA and protein expression levels were significantly decreased, and the results of the ChIP assay suggested that YY1 bound to the EGFR promoter region. The YY1/EGFR axis promotes inflammation and increases oxidative stress in BEAS-2B cells.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3571-3580"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143778674","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Drug repurposing through Biophysical Insights: Focus on Indoleamine 2,3-Dioxygenase and Tryptophan 2,3-Dioxygenase Dual Inhibitors. 通过生物物理见解的药物再利用：专注于吲哚胺2,3-双加氧酶和色氨酸2,3-双加氧酶双重抑制剂。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 Epub Date: 2025-03-26 DOI: 10.1007/s12013-025-01725-2

Priyanga Paranthaman, Ramanathan Karuppasamy, Shanthi Veerappapillai

{"title":"Drug repurposing through Biophysical Insights: Focus on Indoleamine 2,3-Dioxygenase and Tryptophan 2,3-Dioxygenase Dual Inhibitors.","authors":"Priyanga Paranthaman, Ramanathan Karuppasamy, Shanthi Veerappapillai","doi":"10.1007/s12013-025-01725-2","DOIUrl":"10.1007/s12013-025-01725-2","url":null,"abstract":"The kynurenine pathway (KP) plays a pivotal role in dampening the immune response in many types of cancer, including TNBC. The intricate involvement of tryptophan degradation via KP serves as a critical regulator in mediating immunosuppression in the tumor microenvironment. The key enzymes that facilitate this mechanism and contribute to tumor progression are indoleamine 2,3-dioxygenase (IDO1) and tryptophan 2,3-dioxygenase (TDO). Despite attempts to use navoximod as a dual-specific inhibitor, its poor bioavailability and lack of clinical efficacy have hampered its utility. To date, no FDA-approved drugs have advanced for dual targeting of these enzymes. Therefore, this study aimed to repurpose the approved drugs from the DrugBank database as novel IDO1/TDO inhibitors. Initially, 2588 FDA-approved compounds were screened by employing molecular docking and pharmacokinetic profiling. Subsequently, methods such as MM-GBSA calculations and machine learning based analysis precisely identified 20 potential lead compounds. The resultant compounds were then assessed for various toxicity endpoints and anticancer activity. The PaccMann server revealed potent anticancer activity, with sensitivities ranging from 0.203 to 24.119 μM against MDA-MB-231 TNBC cell lines. Alongside, the interaction profile with critical residues, strongly reinforced DB06292 (Dapagliflozin) as a compelling hit candidate. Finally, the reliability of the result was corroborated through a rigorous 200 ns molecular dynamics simulation, ensuring the stable binding of the hit against the target proteins. Considering the promising outcomes, we speculate that the proposed hit compound holds strong potential for the management of TNBC.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3407-3426"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143707836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Down-regulation of ATP8B2 in Foam Cells Inhibits Autophagic Flux and ox-LDL Degradation in Atherosclerosis. 泡沫细胞下调ATP8B2抑制动脉粥样硬化自噬通量和ox-LDL降解。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 Epub Date: 2025-03-28 DOI: 10.1007/s12013-025-01728-z

Xiaodong Miao, Rui Pan, Fei Chang

{"title":"Down-regulation of ATP8B2 in Foam Cells Inhibits Autophagic Flux and ox-LDL Degradation in Atherosclerosis.","authors":"Xiaodong Miao, Rui Pan, Fei Chang","doi":"10.1007/s12013-025-01728-z","DOIUrl":"10.1007/s12013-025-01728-z","url":null,"abstract":"Our study aims to screen and explore the potential molecular mechanisms of atherosclerosis using a comprehensive research approach combining bioinformatics analysis and molecular biology experiments. Bioinformatics analyses were conducted to screen for key genes with significantly differential expression in atherosclerosis. Subsequently, macrophages and foam cells induced from THP-1 cells were utilized to validate the function of these key genes through siRNA knockdown. Molecular biology experiments encompassed reverse transcription polymerase chain reaction (RT-PCR), Western Blotting, immunofluorescence staining, and JC-1 probe detection of mitochondrial membrane potential. ATP8B2, encoding a P4-ATPase, was significantly downregulated in both plaque tissues and circulating macrophages of atherosclerosis patients. This enzyme influences membrane fusion and other dynamic processes by affecting the asymmetric distribution of phospholipids within the bilayer. Knockdown of ATP8B2 expression significantly inhibited autophagic flux in macrophages, manifested by abnormal accumulation of LC3-II and p62 protein levels. Furthermore, downregulation of ATP8B2 expression significantly inhibited the degradation of oxidized low-density lipoprotein (ox-LDL) by macrophages. Simultaneously, reduced ATP8B2 expression led to decreased mitochondrial membrane potential and mitochondrial dysfunction. Our study unveils for the first time the crucial role of ATP8B2 in atherosclerosis, particularly in maintaining autophagic flux, promoting ox-LDL degradation, and sustaining mitochondrial homeostasis.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3451-3463"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143727069","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Protective Effects of Melatonin and Bee Pollen on Hematotoxicity and Hepatorenal Toxicity Induced by Long-Term Intake of Gabapentin in Female Albino Rats. 褪黑素和蜂花粉对长期摄入加巴喷丁引起的雌性白化大鼠血液毒性和肝肾毒性的保护作用。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 Epub Date: 2025-04-14 DOI: 10.1007/s12013-025-01731-4

Hanan A Okail, Mohamed F El Sayed, Mohamed A Adly, Walaa Magdy Abd Elsamei

{"title":"Protective Effects of Melatonin and Bee Pollen on Hematotoxicity and Hepatorenal Toxicity Induced by Long-Term Intake of Gabapentin in Female Albino Rats.","authors":"Hanan A Okail, Mohamed F El Sayed, Mohamed A Adly, Walaa Magdy Abd Elsamei","doi":"10.1007/s12013-025-01731-4","DOIUrl":"10.1007/s12013-025-01731-4","url":null,"abstract":"Gabapentin (GBN) is an anti-seizure medication that is also used to treat nerve pain and other diseases. However, its misuse is currently a growing worry, as it may pose a significant health danger. The present study aimed to evaluate the protective effect of melatonin (MEL) and bee pollen (BP) as antioxidants against GBN-induced hematotoxicity and hepatorenal toxicity in female Albino rats. In this study, fifty-six adult female albino rats were divided into seven groups (n = 8 each), served as control, GBN, MEL, BP, MEL + GBN, BP + GBN, and MEL + BP + GBN treated groups. Results showed that oral administration of GBN resulted in a hematological toxicity as confirmed by a significant reduction in RBCs, Hb concentration, Ht%, MCV, MCH, platelets as well as altering of leukocyte profiles, WBCs, neutrophils, lymphocytes, monocytes, eosinophils and basophils. The biochemical results of liver and kidney functions showed a significant decrease in serum glucose, total protein, triglycerides, urea and uric acid. However, a significant increase in albumin, cholesterol, creatinine as well as ALP, AST, and ALT liver enzymes compared to the control was found. The oral administration of MEL and BP 12 h before GBN mostly ameliorates the altered hematological and biochemical parameters as well as hepatic and renal histopathological architecture to normal levels. In conclusion, Pre-treatment with MEL and BP, individually or together provided protection against the GBN induced changes in the blood parameters as well as hepatorenal structure and function.","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3487-3501"},"PeriodicalIF":2.5,"publicationDate":"2025-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143955009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Cinnamaldehyde Alleviates Alveolar Epithelial Cell Injury in ALI by Inhibiting the CaMKII Pathway. 更正：肉桂醛通过抑制CaMKII通路减轻ALI患者肺泡上皮细胞损伤。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 DOI: 10.1007/s12013-025-01766-7

Lei Liu, Hao Zhang, Siming Chen, Wankang Dian, Zhou Zheng

引用次数: 0

Glycyrrhiza pallidiflora Polysaccharide Ameliorates DSS-Induced Colitis by Protecting Intestinal Barrier Integrity. 甘草多糖通过保护肠道屏障完整性改善dss诱导的结肠炎。

IF 2.5 4区生物学

Cell Biochemistry and Biophysics Pub Date : 2025-09-01 Epub Date: 2025-05-09 DOI: 10.1007/s12013-025-01765-8

Dandan Zeng, Weijie Ren, Bo Zhao, Yuanyuan Li, Jinlong Jiao, Tianlu Mo

引用次数: 0