Cell Biochemistry and Biophysics最新文献

{"title":"WTAP-induced m⁶A Methylation of Atoh8 Promotes Cell Proliferation and Fibrosis in Diabetic Nephropathy.","authors":"Suyu Wang, Henglu Zhang, Bingru Fei, Mei Zhang","doi":"10.1007/s12013-025-01778-3","DOIUrl":"https://doi.org/10.1007/s12013-025-01778-3","url":null,"abstract":"<p><p>Diabetic nephropathy (DN) is a common diabetic complication, which increases morbidity of end-stage renal failure. N6-methyladenosine (m6A) modification has been reported in association with multiple physiological processes, however, its role in diabetic nephropathy is still poorly understood. Here, we found that the levels of m6A modification were up-regulated in both high-glucose-cultured mouse mesangial cells and the renal tissues from db/db mice. The key methyltransferase WT1 associated protein (WTAP) was primarily responsible for the elevated m6A modification. Moreover, WTAP knockdown significantly inhibited the proliferation and fibrosis of mouse mesangial cells (MMCs). Mechanistically, using the combination analysis of MeRIP-Seq and RNA-Seq, we revealed that Atoh8 was a downstream target of WTAP-induced m6A modification. We first revealed that Atoh8 was lowly expressed in renal tissues of DN model mice and HG-induced mesangial cells. WTAP reduced Atoh8 expression by inhibiting Atoh8 mRNA stability. Overexpression of Atoh8 restrained the proliferation and fibrosis of mesangial cells. This study provides novel insights into the role of m6A modification in DN and suggests that WTAP and Atoh8 could serve as potential therapeutic targets for this condition.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Influence of Ubiquitin Mutations over Molecular Markers Contributing to Pathogenesis of Candida albicans.","authors":"Sandeep Kumar Dantuslia, Ankita Doshi, Bhoomi Prajapati, C Ratna Prabha","doi":"10.1007/s12013-025-01772-9","DOIUrl":"https://doi.org/10.1007/s12013-025-01772-9","url":null,"abstract":"<p><p>The emergence of Candida albicans as a life-threatening pathogen and its resistance to available antifungal drugs is a significant global health concern. Previous findings have established that the ubiquitin mutations UbEP42, UbL50P and UbI61T interfere with morphogenesis of C. albicans from commensal yeasts to pathogenic hyphae. The main objective of this study is to investigate the influence of ubiquitin mutations on the molecular markers of morphogenesis and virulence of C. albicans to identify potential targets for therapeutic intervention. The auxotrophic strain BWP17 of C. albicans was transformed by the wild-type ubiquitin gene (UbWT) and its mutants UbEP42, UbS20F, UbA46S, UbL50P and UbI61T cloned under the doxycycline-inducible promoter in the integrative plasmid pTET25-MNC. Induced expression of the mutant forms UbEP42, UbL50P, and UbI61T while inhibiting morphogenesis, reduced chitin deposition, increased β-glucan exposure on the cell wall, decreased the secretion of aspartyl protease, caused the differential expression of cyclins Cln3, Ccn1, Clb2 and certain key transcription factors compared to UbWT. However, UbS20F and UbA46S did not have any influence. These findings demonstrating the disruptive influence of UbEP42, UbL50P, and UbI61T on the levels of molecular markers of morphology and pathogenesis of C. albicans, highlight the importance of a functional ubiquitination system.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Machine Learning Method for Predicting Acute Kidney Injury in Patients with Intracranial Hemorrhage.","authors":"Bo Liu, Di Wu, Yong'An Jiang, Hua Liu","doi":"10.1007/s12013-025-01771-w","DOIUrl":"https://doi.org/10.1007/s12013-025-01771-w","url":null,"abstract":"<p><p>Intracranial hemorrhage (ICH) is a critical and urgent condition in clinical practice. Recent research has highlighted acute kidney injury (AKI) that frequently impacts patient prognosis. For clinicians, early intervention is crucial, and the advancement of machine learning brings promising prospects for predicting this disease. Therefore, this study aims to develop innovative machine learning models for the prediction and diagnosis of acute kidney injury (AKI) in patients with intracerebral hemorrhage (ICH). AKI data of ICH patients were extracted from the Medical Information Mart for Intensive Care IV (MIMIC-IV) database. To construct the models, we utilized various techniques including random survival forest (RSF), elastic network (Enet), Least Absolute Shrinkage and Selection Operator (Lasso), stepwise logistic regression (stepwise LR), and ten machine learning algorithms. Optimal parameters were obtained through a ten-fold crossover, and training and testing groups were employed for the integrated machine models' training and validation. We conducted a quantitative evaluation of the model's performance and assessed its clinical application to determine its advantages. Furthermore, we compared the base model with traditional models such as the Sequential Organ Failure Assessment (SOFA) and the bespoke Simplified Acute Physiology Score (SAPS) II model. A total of 1856 patients with intracerebral hemorrhage (ICH) were enrolled in the study, consisting of 1633 non-AKI patients and 223 AKI patients. Among the various machine learning models tested, XGBoost exhibited the highest predictive accuracy and demonstrated excellent clinical applicability as a standalone model. When combining integrated models, RSF+XGBoost, LR[forward]+Lasso, LR[forward]+RSF, and Lasso+XGBoost, all achieved the highest AUC values (AUC = 1.000). Machine learning models can serve as valuable diagnostic tools in identifying the occurrence of acute kidney injury (AKI) in intracerebral hemorrhage (ICH) cases. Whether utilized individually or in combination, these models have the potential to assist clinicians in proactively developing effective interventions.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144118481","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Investigate the Effect of ZFP64 on mRNA Expression of HBG Based on Bioinformatics and Experimental Validation.","authors":"Siqi Huang, Jianfeng Wu, Yinghong Yang, Mingming Zhu, Lihao Chen, Shunhan Zhang, Yi Yang, Xiaofang Sun, Yingjun Xie","doi":"10.1007/s12013-025-01776-5","DOIUrl":"https://doi.org/10.1007/s12013-025-01776-5","url":null,"abstract":"<p><p>γ-globin genes (HBG1 and HBG2) are usually expressed during fetal life, and almost no expression after birth. Therefore, the reactivation of HBG is a key target for the treatment of hemoglobinopathy. ZFP64 is a C2H2 type zinc finger transcription factor, which has been shown to play an important role in the maintenance of gene expression in mixed lineage leukemia, and other C2H2 type zinc finger transcription factors (such as ZFP410 and ZFP644) have been shown to regulate the expression of fetal hemoglobin (HbF) in thalassemia. This study aims to investigate the effect of ZFP64 on mRNA expression of HBG. We performed bioinformatics analyses using the Gene Ontology (GO), Kyoto Encyclopedia of Genes and Genomes (KEGG), and protein-protein interaction (PPI) networks to identify genes and transcription factors associated with ZFP64. ZFP64 was knocked out in K562 and HUDEP-2 cell lines by CRISPR-Cas9 electroporation, and the transcription levels of ZFP64, HBB and HBG were analyzed. In undifferentiated and 7-day differentiated HUDEP-2 cells, knocking down ZFP64 resulted in a 1.5-fold and 2.5-fold increase in HBG mRNA expression, respectively (p < 0.05). These findings suggest that ZFP64 is a potential regulator of HBG expression and warrants further investigation as a therapeutic target in hemoglobinopathies.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144109344","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Native Fluorescence Characterization of Blood Plasma for Early Diagnosis of Oral Potentially Malignant Disorders.","authors":"Pravda Chidambaranathan, Rajvikram Natarajan, Divyambika Catakapatri Venugopal, Saravanan Ramachandran, Jamal Mohamed Thoufeeq, Anandh Sundaramoorthy, Singaravelu Ganesan, Aruna Prakasarao","doi":"10.1007/s12013-025-01779-2","DOIUrl":"https://doi.org/10.1007/s12013-025-01779-2","url":null,"abstract":"<p><p>Oral potentially malignant disorders (PMD) are heterogenous group of oral conditions characterized by increased risk of malignant transformation that are commonly diagnosed clinically and treated. Tissue biopsy is an invasive tool for diagnosis of oral PMDs. Native fluorescence spectroscopy of biofluids has been considered as an alternative and/or complimentary tool in the characterization of tissues of various pathological conditions. In the present study, it is primarily aimed to characterize different fluorophores present in the blood plasma of normal subjects and PMD patients, using excitation-emission matrix (EEM) and to compare their diagnostic potentials in delineating PMD cases from normal subjects. From EEM measurements of blood plasma, it was observed that there is a maximum emission around 340 ± 5 nm at 307 ± 3 nm excitation for normal subjects and around 337 ± 5 nm emission at 300 ± 3 nm excitation for PMD patients. In addition to the maxima, the contours exhibit other secondary emissions such as 480 ± 5 nm at 335 ± 3 nm excitation for both normal and PMD blood plasma. It was also observed that an emission at 520 ± 5 nm for 440 ± 3 nm excitation is seen for PMD blood plasma and the same is absent in the cases of normal blood plasma. The three contours centred around 340, 480 and 520 nm may be attributed to emissions from tryptophan, NADH and FAD, respectively. Results of stepwise multiple linear discriminant analysis reveal that fluorescence spectral features of NADH classify samples more effectively with 100% sensitivity and 95% specificity.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144101228","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Syringic Acid in Canarium odontophyllum for Diabetes and Obesity - A Review.","authors":"Gilbert Ringgit, Bo Eng Cheong, Muhammad Dawood Shah, Noor Atiqah Aizan Abdul Kadir, Shafiquzzaman Siddiquee","doi":"10.1007/s12013-025-01773-8","DOIUrl":"https://doi.org/10.1007/s12013-025-01773-8","url":null,"abstract":"<p><p>Syringic acid (SA) is a phenolic compound with a significant role in the treatment of diabetes and obesity. Syringic acid possesses anti-obesity and anti-diabetic properties; however, the potential of syringic acid derived from the native Bornean fruit Canarium odontophyllum (C. odontophyllum) for managing diabetes and obesity remains undocumented. This brief discussion explores the possible mechanisms associated with syringic acid's structure and its potential therapeutic effects in managing diabetes and obesity. The relevant information is gathered from previous reports on syringic acid, related to molecular docking studies involving syringic acid-associated enzymes and protein residues. The potential mechanism of syringic acid derived from C. odontophyllum with chemical structure characterized by a benzene ring with hydrogen bonds and its high affinity for enzymes and protein residues targeting diabetes and obesity, including hexokinase 2 (HK2), glycogen synthase kinase (GSK), 2BEL, protein kinase D (PKD), insulin receptor substrate-1 (IRS-1), and insulin receptor beta (IR-β). This review paper provides alternative insights into syringic acid derived from the seasonal fruit of native Bornean fruit associated with molecular docking, structural advantages and mechanism of action in diabetes treatment.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075109","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A Potent Plant-Derived Chitinase: Structural Informatics and Insecticidal Activity against Helicoverpa armigera.","authors":"Shah Rukh, Ahmed Akrem, Qamar Saeed, Sohaib Mehmood, Nosherwan Adil, Tazeen Rao, Muhammad Nasir, Uzma Ishaq, Aqal Zaman, Najeeb Ullah, Mohibullah Shah","doi":"10.1007/s12013-025-01777-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01777-4","url":null,"abstract":"<p><p>Helicoverpa armigera (cotton bollworm) is a globally distributed lepidopteran pest that causes estimated annual agricultural losses exceeding 5 billion USD. While chemical pesticides remain the primary control strategy, their prolonged use has led to significant environmental contamination, development of widespread insecticide resistance, and non-target organism toxicity. These limitations underscore the critical need for plant-derived biopesticides that offer target specificity, environmental biodegradability, and sustainable pest management solutions without promoting resistance development. Here, we elucidate the insecticidal potential of Nelumbo nucifera Chitinase (NnChi) against the insect H. armigera through structural informatics and in-vivo insecticidal bioassays. SDS-PAGE showed a single band of ~32 kDa, and LC-MS/MS analysis depicted a fragment of 10 amino acids with 100% identity with Family 19 Class I Chitinase of Mangifera indica. NnChi-predicted structure revealed its two domains (ChB D, Cat D) connected through linker region and docking analysis of both these domains with (GlcNAc)₄ showing binding affinities of -5.6 kcal/mol and -7.0 kcal/mol, respectively. MD simulation (100 ns) showed that 4 residues (RQCR) of ChB D and 4 residues (NRIP) of Cat D contributed to binding with (GlcNAc)₄. To the best of our knowledge, we are reporting the molecular interactions of both domains (ChB D and Cat D) with (GlcNAc)₄ via simulation studies for the first time. These computational findings were further verified through insecticidal assay. Significant larval mortality of H. armigera was observed from 3^rd-6^th instar against 15 µg/g NnChi treatment. Among life cycle parameters, larval and pupal duration, adult eclosion, larval and pupal weight are significantly decreased in a concentration-dependent manner as compared to control. Our integrated structural-functional characterization demonstrates that NnChi exhibits significant insecticidal activity against Helicoverpa armigera. These findings establish NnChi as a promising biopesticide candidate worthy of further investigation.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075093","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effects of Turmeric on Manganese-induced Redox Imbalance in Testicular Tissues: Histo-micrometric and Biochemical analyses.","authors":"Fatima Bashir Ahmad, Chaman Ara, Shaukat Ali, Shagufta Andleeb, Hafiz Abdullah Shakir, Faheem Nawaz, Asmatullah","doi":"10.1007/s12013-025-01763-w","DOIUrl":"https://doi.org/10.1007/s12013-025-01763-w","url":null,"abstract":"<p><p>Manganese is the most overlooked chemical element, though it is the 5^th most abundant metal in earth' crust. Despite being important in the body's mechanisms, bioaccumulation of manganese in living organisms can be noxious. The current study aimed to evaluate the effects of manganese on male mice's reproductive parameters, and turmeric was used as a remedial agent against manganese-induced potential subchronic toxicity. Healthy male mice were distributed into seven groups (n = 10), Control (untreated), Vehicle control; VC-I (0.1 ml of saline solution), Mn-I (5 mg/Kg BW), Mn-II (10 mg/Kg BW), Mn-III (20 mg/Kg BW), Mn + T (20 mg of Mn/Kg +100 mg turmeric/Kg BW) and Vehicle control; VC-II (0.1 ml of olive oil) via oral gavage routinely, once a day. The experiment was continued for 28 days. Before administrations, the antioxidant capacity of turmeric was evaluated via FRAP, TPC & GC-MS assays. Mice were acclimatized for 10 days after dosing, then euthanized and samples (testis & blood) were recovered. Morphological observations showed minute morphological changes in testes as compared to controls. Morphometric analysis revealed average body weight, testis weight and size of Mn-intoxicated mice were reduced remarkably (P ≤ 0.05) in comparison with the control group. Sideways Mn + T group showed non-significant changes in both parameters. Hematological, micrometric findings and serum testosterone, luteinizing hormone and follicle stimulating hormone were significantly different (P ≤ 0.05) in Mn-exposed groups against controls. These alterations were concomitant with histological variations in Mn-treated groups. While such deviations were less obvious in the Mn + T-administered group. The aforesaid findings deduced that the turmeric supplementation manifested improvements in most of the histo-micrometric, hematological, steroidal parameters and enzymatic indices of mice through its antioxidant action.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144075106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Icariside II Alleviates Chondrocyte Inflammatory Injury by Inhibiting the TNIP2/NF-κB Pathway.","authors":"Jiajun Mei, Hongkui Ke, Junsong Zhu","doi":"10.1007/s12013-025-01769-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01769-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143967727","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Palmitic Acid-induced Renal Injury: Unravelling the Molecular Mechanisms and the Protective Role of Lycopene.","authors":"Adewale S James, Emmanuel I Ugwor, Adio J Akamo, Dorcas I Akinloye, Ayokulehin M Kosoko, Boluwatife A Olagunju, Emmanuel O Ezenandu, Charity C Amaogu, Victoria Adebiyi, Funmilola C Thomas, Regina N Ugbaja","doi":"10.1007/s12013-025-01775-6","DOIUrl":"https://doi.org/10.1007/s12013-025-01775-6","url":null,"abstract":"<p><p>To understand the role of palmitic acid overload on renal physiology, we exposed female rats to palmitic acid (PA) beyond the physiological range reminiscent of a high-fat western diet. We then treated the rats with graded doses of lycopene (Lyc) to evaluate its potential remedial effect against PA-induced renal injury. Twenty-four rats were randomized into four groups as control (received vehicles; Free Fatty Acid Free Bovine Serum Albumin (BSA) and Olive oil; PA only [received 5 mM BSA-complexed PA]; while the other two groups received 10 and 20 mg/kg body weight of Lyc along with the BSA-complexed PA. The Olive oil-reconstituted Lyc was commenced at the seventh week of intraperitoneal PA injection (uninterrupted) until the nineth week. Our results show that palmitic acid PA overload caused renal lipid dystrophy, characterized by decreased cholesterol, triglycerides, and phospholipids. We also observed elevated activities of lactate dehydrogenase and decreased activity of alanine aminotransferase. The activities of superoxide dismutase, myeloperoxidase, and malondialdehyde levels increased, while glutathione peroxidase activity decreased significantly. Furthermore, PA-induced disruption of cellular electrolytes is characterized by decreased calcium, chloride, sodium, and magnesium ions, and elevated activities of Na⁺/K⁺, and Ca²⁺/Mg²⁺ ATPases. Western blot analysis shows decreased Nrf2 expression, while NF-^KB and Toll -Like Receptor 4 (TLR4) increased. Furthermore, the mRNA expression of TLR4, IL-6, TNF-alpha, and IL-1β increased, while IL-10 decreased in PA only group. However, Lyc treated groups exhibits meaningful ameliorative potentials by abating oxidative stress, inflammation, lipid dystrophy, and iono-dysregulation. This study suggests lycopene supplementation might abate PA -invoked disruption of lipid metabolism, inflammatory signal propagation and disruption of innate antioxidant systems in female albino rats.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":1.8,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143956735","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}