Cell Biochemistry and Biophysics最新文献

{"title":"Correction: Naringin Potentiates Docetaxel-Induced Apoptosis in Breast Cancer Cells via Transient Interaction with the TUBB3 GTP-Binding Site.","authors":"Miguel Kativa, Agilan Balupillai, T Jayakumar, Joen-Rong Sheu","doi":"10.1007/s12013-026-02066-4","DOIUrl":"10.1007/s12013-026-02066-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4681"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147621483","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Kinetic and Apparent Thermodynamic Analysis of Phaseolus Vulgaris Leucoagglutinin Binding to Cell-Surface Glycans in SR Lymphoma Cells Using a Fixation-Modified Cell-ELISA.","authors":"Qais M Abdulhameed, Hassanain K Al-Bairmani, Ahmed Majeed Al-Shammari","doi":"10.1007/s12013-026-02145-6","DOIUrl":"10.1007/s12013-026-02145-6","url":null,"abstract":"<p><p>Cell-surface glycosylation plays an important role in lectin-mediated molecular recognition and is frequently altered in malignant cells. In this study, a fixation-modified cell-ELISA was developed to enable stable immobilization of non-adherent cells and to compare the binding behavior of Phaseolus vulgaris leucoagglutinin (PHA-L) toward SR lymphoma cells and normal human peripheral blood mononuclear cells (PBMCs). Saturation binding and time-course assays were performed at 5, 15, 25, and 37 °C, and the resulting whole-cell ELISA data were analyzed using Langmuir, Van't Hoff, Arrhenius, and Eyring models to obtain comparative apparent kinetic and thermodynamic descriptors. Saturation binding analysis showed lower apparent equilibrium dissociation constants (Kd_app) and higher maximum binding responses (OD_max) for SR lymphoma cells than for normal PBMCs under identical experimental conditions, indicating a stronger apparent PHA-L binding response. Time-course analysis demonstrated faster apparent binding kinetics in SR lymphoma cells, characterized by higher apparent observed and association rate constants (k_obsapp and k_onapp), lower apparent dissociation rates (k_offapp), and shorter observed association half-times. Model-based thermodynamic analysis suggested that the apparent binding process was endothermic and predominantly entropy-favored in both cell types. Arrhenius and Eyring analyses further provided comparative apparent energetic descriptors, with SR lymphoma cells exhibiting lower apparent Arrhenius-derived energetic parameters and lower apparent activation enthalpy than normal PBMCs under the experimental conditions. Because these parameters were derived from mathematical analysis of whole-cell ELISA measurements rather than direct real-time biophysical techniques, they should be interpreted as apparent comparative descriptors rather than intrinsic molecular kinetic or thermodynamic constants. Overall, the fixation-modified cell-ELISA provided a feasible platform for exploratory comparative evaluation of PHA-L binding to suspension cells and revealed measurable differences in the apparent binding behavior of SR lymphoma cells and normal PBMCs. These findings support the potential utility of this approach for comparative lectin-cell interaction studies and future investigations of cell-surface glycosylation.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4555-4570"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148757223","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Phytochemical, Computational and In Vitro Evaluation of the Oocysticidal Activity of Pleurotus ostreatus Extract Against Cryptosporidium parvum.","authors":"Amal M Marey, Mohamed A Dkhil, Shaimaa M Kasem, Seifeldin Elabed, Felwa A Thagfan, Mohga S Abdalla","doi":"10.1007/s12013-026-02126-9","DOIUrl":"10.1007/s12013-026-02126-9","url":null,"abstract":"<p><p>Cryptosporidiosis is a significant zoonotic parasite disease caused by Cryptosporidium parvum, impacting both humans and animals, with little therapeutic alternatives available. This work examined the phytochemical composition, antioxidant capacity, in silico antiparasitic potential, and in vitro oocysticidal activity of methanolic Pleurotus ostreatus extract (POE). Fruiting bodies extract was analyzed for phytochemical constituents. The total amounts of phenolics, flavonoids, tannins, and saponins were quantified spectrophotometrically, while antioxidant activity was evaluated by the reducing power test. Gas chromatography-mass spectrometry (GC-MS) chemical profiling revealed 18 identified compounds. Molecular docking was performed on three validated targets of C. parvum: N-myristoyltransferase (NMT), calcium-dependent protein kinase 1 (CDPK1), and lysyl-tRNA synthetase (KRS) to assess possible antiparasitic interactions. Several sterol derivatives produced favorable predicted binding scores, including Ergost-7-en-3-ol (3β) and ergosterol toward NMT (- 9.87 and - 9.28 kcal/mol, respectively), compared with nitazoxanide under the docking conditions. Interaction profiling identified possible ligand-target interactions with proteins involved in protein lipidation, Ca²⁺-dependent signaling, and translation pathways, but these mechanisms remain computational hypotheses pending biochemical validation. ADMET predictions suggested minimal systemic absorption for sterols, which may favor gut-localized activity and prolonged intestinal exposure against C. parvum while minimizing systemic toxicity. In vitro studies revealed a concentration- and time-dependent decrease in C. parvum oocyst numbers and viability. At a concentration of 1000 µg/mL, POE diminished oocyst counts by nearly 71% after 48 h and lowered viability to 37.41%, demonstrating greater reduction of oocyst count and viability than nitazoxanide under comparable conditions with LC₅₀ of 460 µg/mL. These findings collectively underscored P. ostreatus as a potential natural antiparasitic agent, indicating that its sterol-rich extract may demonstrate oocysticidal activity via multi-target molecular interactions and direct impacts on oocyst integrity.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4347-4367"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148720730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Machine Learning-Derived Sarcopenia Signature Identifies High-Risk Molecular State in T2D Skeletal Muscle.","authors":"Qiyao Zhao, Qiwang He, Sining Wang, Hanzhi Wang, Zheyuan Zhang, Zhi Shang, Yue Dong","doi":"10.1007/s12013-026-02138-5","DOIUrl":"10.1007/s12013-026-02138-5","url":null,"abstract":"<p><p>Sarcopenia is common in type 2 diabetes (T2D), but whether sarcopenia-associated transcriptomic features can be detected in diabetic skeletal muscle remains unclear. A machine-learning signature combining 26 gene-expression features and one ssGSEA-derived aggregate feature was developed in GSE111016 and GSE226151 and independently evaluated in GSE111010. The frozen signature was projected onto T2D bulk transcriptomic cohorts and a nine-donor single-nucleus RNA-sequencing dataset. Its association with lower-extremity physical performance was further evaluated in GSE144304. The model achieved AUCs of 0.906 in the training cohort and 0.762 in the validation cohort. In T2D skeletal muscle, the high-risk molecular state was associated with inflammatory, extracellular-matrix, adhesion, and stress-response programs, with SESN3 and VCAM1 identified as candidate genes associated with shared pathological processes. In GSE144304, a higher frozen signature score was associated with lower SPPB after adjustment for age, sex, and BMI. Single-nucleus analysis provided exploratory cellular context for potential variation in cell-type and myonuclear distributions. The frozen molecular signature can identify a high-risk molecular state in T2D skeletal muscle.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4461-4477"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760110","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In vivo and In Silico Assessment of Acampe papillosa (Lindl.) Lindl. Seed Extract: A Multi-Pharmacological Approach.","authors":"Nusaiba Nujhat, Jannatul Naima Meem, Shyra Tasmia, Nowsheen Sayra, Md Arifuzzaman, Kamrul Hassan, Ali Ahsan Md Mozahid, S M Moazzem Hossen","doi":"10.1007/s12013-026-02154-5","DOIUrl":"10.1007/s12013-026-02154-5","url":null,"abstract":"<p><p>Acampe papillosa (Lindl.) Lindl., an epiphytic orchid, has been traditionally utilized in ethnomedicine for the management of pyrexia, otalgia, rheumatism, and neuralgia. This work intends to figure out the in vivo neuropharmacological, antinociceptive, and antipyretic potentials of the methanolic extract of A. papillosa seeds (ME-APS) along with computational approaches. Phytochemical screening of ME-APS was analyzed through GC-MS. Elevated plus maze (EPM) and hole board (HBT), forced swim (FST), and tail suspension (TST) tests were used to evaluate anxiolytic and antidepressant effects, whereas acetic acid-induced writhing and formalin-induced paw licking were employed to assess antinociceptive action. Antipyretic activity was examined on the brewer's yeast-induced pyrexia model. Molecular docking and ADME/T analyses were conducted to support the experimental findings. GC-MS analysis revealed 13 prominent bioactive compounds within ME-APS. The extract demonstrated significant, dose-dependent antipyretic and central analgesic activities (p < 0.001) at dosages of 200 and 400 mg/kg. Furthermore, at 200 mg/kg, the extract manifested robust peripheral analgesic and antidepressant-like effects (p < 0.01), alongside a moderate anxiolytic response in the HBT model. Molecular docking studies supported the experimental findings by identifying favorable interactions between selected phytoconstituents and protein targets associated with pain, pyrexia, and neuropharmacological pathways. Based on the computational analysis, the identified compounds exhibited binding affinities ranging from - 4.4 to - 10.1 kcal/mol across the targeted proteins. Furthermore, in silico ADME/T analysis predicted favorable pharmacokinetic characteristics and low toxicity risks for the identified phytoconstituents. Overall, the findings suggest that A. papillosa seeds contain bioactive constituents that may contribute to neuropharmacological, antinociceptive, and antipyretic activities. Further studies, including detailed biochemical and receptor-based investigations, as well as long-term experimental models, are required to confirm these effects.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4627-4643"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148786616","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Redox Imbalance, Oxidative DNA Damage, and Mitochondrial Membrane Depolarization Induced by Pervari Honey in SH-SY5Y Neuroblastoma Cells.","authors":"Muazzez Derya-Andeden, Pinar Altin-Celik, Hamiyet Donmez-Altuntas","doi":"10.1007/s12013-026-02164-3","DOIUrl":"10.1007/s12013-026-02164-3","url":null,"abstract":"<p><p>Neuroblastoma remains a challenging malignancy in which redox imbalance and mitochondrial dysfunction represent potential cellular vulnerabilities. This study investigated the redox-associated cytotoxic effects and mitochondrial responses of Pervari honey (PH), a region-specific natural product from Türkiye, in SH-SY5Y neuroblastoma cells. MTT-based viability was assessed in SH-SY5Y neuroblastoma cells and L929 fibroblasts, used as a non-cancerous control cell model, after 48 h of PH exposure. Redox status was evaluated by measuring intracellular reactive oxygen species (ROS), total antioxidant capacity (TAC), total oxidant status (TOS), oxidative stress index (OSI), glutathione (GSH), malondialdehyde (MDA), and 8-hydroxy-2'-deoxyguanosine (8-OHdG). Mitochondrial membrane potential (ΔΨm) was analyzed using the JC-10 assay. Expression levels of apoptosis- and antioxidant-related genes were determined by RT-qPCR. Comparative viability analysis showed that L929 fibroblasts were less affected by PH exposure than SH-SY5Y cells, and the IC50 value of PH was not reached in L929 cells within the tested concentration range. Treatment increased ROS production and shifted the oxidant/antioxidant balance toward oxidative stress, as indicated by increased TOS and OSI and decreased TAC and GSH levels. Elevated MDA and 8-OHdG levels confirmed oxidative damage to lipids and DNA. Additionally, PH reduced mitochondrial membrane potential and altered the expression of apoptosis- and antioxidant-related genes, including cytochrome c (Cyt-c), p53, SOD1, and CAT. PH exerted cytotoxic effects in SH-SY5Y cells through redox imbalance and mitochondrial dysfunction. These findings suggest that PH may influence redox-sensitive pathways in neuroblastoma cells. However, further studies including compositional analysis and appropriate controls are required to clarify the underlying mechanisms.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4669-4680"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148872523","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Association of Epithelial-to-Mesenchymal Transition Related Markers With Clinical Outcome in Non-Muscle Invasive and Muscle Invasive Bladder Cancer Patients.","authors":"R Singh, Singh Up, Minal Garg","doi":"10.1007/s12013-026-02106-z","DOIUrl":"10.1007/s12013-026-02106-z","url":null,"abstract":"<p><p>Clinical significance of epithelial-to-mesenchymal transition (EMT)-related markers was evaluated in a cohort of NMIBC (non-muscle invasive bladder cancer) and MIBC (muscle invasive bladder cancer) patients. Real-time quantitative polymerase chain reaction (RT-qPCR) and immunohistochemical (IHC) staining were carried out in 100 tumor specimens [59 NMIBC and 41 MIBC]. The expressions of the epithelial marker, mesenchymal markers and EMT-activating transcription factors (EMT-ATFs) were determined at mRNA and protein level followed by their statistical associations with clinicohistopathological variables of the patients. Expression of E-cadherin at mRNA level exhibited significant association with tumor type in NMIBC cohort and with age of MIBC patients. Association of mRNA levels of N-cadherin was reported with smoking/tobacco chewing status (S/TC) of NMIBC patients and with tumor type in MIBC cohort. Membranous expression of Vimentin protein showed significance with age of MIBC patients. Gene expression of Snail showed relevance with tumor type and S/TC status of NMIBC patients whereas its protein (nuclear) expression exhibited significance with tumor grade in NMIBC cohort. Nuclear expression of Slug showed significance with S/TC status of NMIBC patients. The study reported significances of gene levels of Twist with tumor stage and age of MIBC patients while its protein (nuclear) expression with tumor grade in NMIBC cohort. Zeb-1 mRNA levels exhibited statistical relevance with tumor stage while its protein (nuclear) expression with tumor grade in NMIBC patients. Survival analysis identified Vimentin as the predictor of short progression free survival (PFS) and short overall survival (OS) in MIBC patients. Study established altered EMT profile as an independent predictor of short recurrence free survival (RFS) in NMIBC patients and of short PFS and OS in MIBC patients. Observed significances of EMT-related markers with patients' clinicohistopathological variables as well as with their survival outcome may strengthen risk stratification and hence could be of importance in the clinical management of bladder cancer patients.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4089-4103"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148314938","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated Transcriptomic Profiling and In Vitro Evaluation of Garcinia Mangostana Phytochemicals Against Cryptosporidium Parvum.","authors":"Asmaa A Abdelhafeez, Mohamed A Dkhil, Shaimaa M Kasem, Seifeldin Elabed, Felwa A Thagfan, Mohga S Abdalla","doi":"10.1007/s12013-026-02107-y","DOIUrl":"10.1007/s12013-026-02107-y","url":null,"abstract":"<p><p>Cryptosporidium parvum is a major cause of severe diarrheal disease, yet therapeutic options remain limited. This study integrated host-parasite transcriptomic profiling, GC-MS phytochemical profiling, computational screening, and in vitro experimental validation to evaluate anti-Cryptosporidium phytochemicals from Garcinia mangostana extract (GME). Host epithelial transcriptional responses (GSE2077) during C. parvum infection were analyzed to map infection-associated host reprogramming. A curated library of 30 identified phytochemicals was computationally docked against prioritized parasite-relevant targets: calcium-dependent protein kinase 1 (CDPK1), C. parvum 1-phosphatidylinositol 4-kinase [PI(4)K; cgd8_4500/UniProt Q5CVD3, AlphaFold model AF-Q5CVD3-F1], and cleavage and polyadenylation specificity factor 3 (CPSF3). The anti-Cryptosporidium efficacy of GME was subsequently evaluated in vitro by assessing C. parvum oocyst count and viability over 48 h. Transcriptomic analysis suggested a biphasic pattern of host reprogramming involving inflammatory signaling, sterol metabolism, Wnt signaling, and cell-cycle regulation. GC-MS analysis identified sterols and fatty acid derivatives, including stigmasterol, campesterol, and ethyl iso-allocholate. Molecular docking against the C. parvum PI(4)K model predicted campesterol (NP028) and ethyl iso-allocholate (NP029) as leading PI(4)K-oriented candidates, with mean affinities of - 7.97 ± 0.59 and - 7.72 ± 0.61 kcal/mol, respectively, while NP008 showed a predicted multi-target interaction profile, particularly against CPSF3. In vitro assays showed a significant, concentration- and time-dependent reduction in C. parvum oocyst viability, with an LC50 of 830 µg/mL after 48 h. Overall, the parasite-specific docking and oocysticidal data support GME phytochemicals as promising anti-Cryptosporidium candidates and generate testable hypotheses for CDPK1, PI(4)K, or CPSF3 involvement, while direct intracellular infection assays and recombinant enzyme inhibition studies are required to confirm target engagement.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4105-4129"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429532","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Mechanistic Insights into Aldose Reductase-Dependent Modulation of Hypoxia-Inducible Factor-1α in Promoting Renal Fibrosis.","authors":"Wenke Zhao, Haoyu Wang, Jingjing Yan, Lutan Zhou, Wei Xu, Xianwei Li","doi":"10.1007/s12013-026-02149-2","DOIUrl":"10.1007/s12013-026-02149-2","url":null,"abstract":"<p><p>Renal fibrosis (RF) represents the common pathological endpoint of all chronic kidney diseases. According to recent evidence, impaired mitophagy, which facilitates epithelial-mesenchymal transition (EMT) in renal tubular epithelial cells (RTECs), substantially contributes to RF progression, although the underlying mechanisms remain unclear. We previously demonstrated that mitophagy mediated by aldose reductase (AR) promotes EMT of hepatocytes. Further investigations are warranted to elucidate whether AR affects RF and EMT in RTECs through the regulation of impaired mitophagy. In the in vivo study, AR knockout remarkably reduced Phosphoinositide 3-kinase (PI3K) and serine/threonine kinase (AKT) phosphorylation and attenuated hypoxia-inducible factor-1α (HIF-1α) expression, while increasing Pink1 and Parkin expression in unilateral ureteral obstruction (UUO)-exposed renal tissues. These changes were linked with a higher expression ratio of microtubule-associated protein 1 light chain 3 (LC3) II/I; low p62 expression; decreased Snail, α-smooth muscle actin‌ (α-SMA), and vimentin expression; enhanced E-cadherin expression; and a reduction in mitochondrial damage, which collectively contributed to mitigate RF. In the in vitro study, knockdown of AR through siRNA or pharmacological inhibition markedly reduced transforming growth factor-beta 1 (TGF-β1)-induced HIF-1α expression, inhibited PI3K/AKT pathway activation, restored mitochondrial autophagy function, decreased levels of reactive oxygen species (ROS) and mitochondrial permeability transition pore (MPTP) opening, and increased mitochondrial membrane potential (ΔΨm) and adenosine triphosphate (ATP) production, thereby reversing EMT in RTECs. Conversely, AR overexpression exacerbated TGF-β1-induced HIF-1α expression, enhanced PI3K/AKT pathway activation, lowered efficiency of mitophagy, increased MPTP opening and ROS levels, reduced ΔΨm and ATP production, and promoted EMT. These findings highlight AR's role as a facilitator of EMT in RTECs and emphasize its critical contribution to RF progression. This process may be mediated by AR-induced expression of HIF-1α, which stimulates the PI3K/AKT signaling pathway, leading to inhibition of Pink1 and Parkin expression, ultimately decreasing mitophagy occurrence in RTECs.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4599-4611"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148758333","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Phytochemical Profiling and Multifunctional Bioactivities of Pergularia tomentosa L. Latex: Angiogenic Potential via the CAM Model, Accelerated Burn Wound Healing in Rats, and In Vitro Antioxidant and Antibacterial Evaluation.","authors":"Khaoula Segueni, Atef Chouikh, Yahia Khelef, Mohammed Laid Tlili, Abderrhmane Bouafia, Abir Benaissa, Hatem M M Mazroaa, Khuzama M S Al-Essa, Salah Eddine Laouini, Farid Menaa","doi":"10.1007/s12013-026-02120-1","DOIUrl":"10.1007/s12013-026-02120-1","url":null,"abstract":"<p><p>Burn wounds represent a major clinical challenge due to delayed healing, high infection risk, and the limitations of current therapeutic agents. This study investigated the phytochemical composition and multifunctional bioactivities of Pergularia tomentosa L. latex as a potential natural therapeutic for burn wound management. Phytochemical screening identified phenolic compounds, flavonoids, tannins, alkaloids, terpenoids, and saponins, with substantial quantities of lipids (105.95 ± 0.01 mg ESO/g), proteins (97.91 ± 0.02 mg EBSA/g), and carbohydrates (229.92 ± 0.03 mg EG/g), supported by FTIR spectral analysis. The latex demonstrated a sun protection factor of 12.78, moderate hydroxyl radical scavenging activity (IC₅₀: 102.16 ± 7.00 mg/mL), antioxidant capacity (46.64 ± 0.003 EAA/g), and erythrocyte membrane protection against oxidative hemolysis. Antibacterial assays revealed broad-spectrum activity with a minimum inhibitory concentration of 0.9375 mg/mL against all tested strains. The chorioallantoic membrane assay confirmed marked pro-angiogenic activity with enhanced neovascularization and vascular branching at lower concentrations. In vivo evaluation in a rat burn model showed that topical formulations containing 2% and 7% latex significantly accelerated wound closure and tissue repair over 22 days compared with controls (p < 0.001), with near-complete re-epithelialization observed in the 7% formulation group. These findings support the therapeutic potential of P. tomentosa latex as a multifunctional natural candidate for burn wound management and tissue regeneration.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4299-4320"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719636","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}