Cell Biochemistry and Biophysics最新文献

{"title":"Therapeutic Insights into the Hepatoprotective Effect of Strobilanthes callosa.","authors":"Divyanshi Chauhan, Falguni Goel, Vipin Kumar Garg","doi":"10.1007/s12013-026-02100-5","DOIUrl":"10.1007/s12013-026-02100-5","url":null,"abstract":"<p><p>The present study was conducted to evaluate the hepatoprotective potential of Strobilanthes callosa using a well-established rat model of experimentally induced liver injury. The investigation assessed biochemical, hematological, oxidative stress, and histopathological parameters to determine the protective efficacy of the plant extract. Body weight was recorded at the beginning and end of the treatment period, while liver weight was measured after sacrifice to evaluate physiological changes associated with hepatic injury. Serum biochemical markers, including alanine aminotransferase (ALT), aspartate aminotransferase (AST), alkaline phosphatase (ALP), bilirubin, albumin, and triglycerides, were analyzed using standardized enzymatic assay kits. Treatment with S. callosa significantly reduced serum ALT, AST, and ALP levels respectively, compared with the toxic control group (p < 0.05), indicating improved liver function. Bilirubin levels were significantly decreased, whereas albumin levels were restored toward normal values following treatment. Oxidative stress biomarkers revealed a significant reduction in malondialdehyde (MDA) levels by along with increased antioxidant defense through restoration of catalase and glutathione levels respectively (p < 0.05). Hematological parameters, including hemoglobin (Hb), red blood cell (RBC), white blood cell (WBC) counts, packed cell volume (PCV), and differential leukocyte count, were evaluated to assess systemic toxicity, inflammatory response, and general physiological status. Histopathological examination of liver tissues using hematoxylin and eosin staining demonstrated marked improvement in hepatic architecture, with reduced necrosis, fatty degeneration, inflammatory infiltration, and tissue disorganization in treated groups compared with toxin-treated animals. Statistical analysis was performed using GraphPad Prism 8.0, with results expressed as mean ± SD. Data were analyzed using one-way and two-way ANOVA followed by appropriate post hoc tests, and differences were considered statistically significant at p < 0.05.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4001-4014"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148315125","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Microalgae-Mediated Synthesis of Gold Nanoparticles from Indonesian Chlorella vulgaris InaCC M205 with Potential Anticancer Properties for Biomedical Application.","authors":"Nunik Gustini, Debora Christin Purbani, Mega Ferdina Warsito, Riyona Desvy Pratiwi, Diah Radini Noerdjito, Fiddy Semba Prasetiya, Nico Rahman Caesar, Uun Yanuhar, Firli Rahmah Primula Dewi, Arien Dwitrie, A'liyatur Rosyidah","doi":"10.1007/s12013-026-02103-2","DOIUrl":"10.1007/s12013-026-02103-2","url":null,"abstract":"<p><p>Sustainable nanomaterial synthesis has emerged as a critical strategy to reduce the environmental burden associated with conventional chemical synthesis method. Microalgae-derived biomolecules offer a promising platform for the green production of metal nanoparticles due to their rich bioactive compounds capable of acting as natural reducing and stabilizing agents. Here, we report the eco-friendly synthesis of gold nanoparticles (AuNPs) using extract of Indonesian microalga Chlorella vulgaris extract. To optimize the synthesis process, the effects of precursor-to-extract ratio, temperature, and incubation time were evaluated. Optimal synthesis of C5-AuNPs was obtained at 37 °C for 20 h with precursor to extract ratio of 6:4, resulting in moderately stable C5-AuNPs characterized by a surface plasmon resonance (SPR) peak at 541 nm. Furthermore, Fourier-transmission infra-red (FT-IR) analysis revealed the involvement of functional groups of C. vulgaris extract in the interaction with Au⁺ during the production of C5-AuNPs. Transmission electron microscopy (TEM) demonstrated the formation of uniformly spherical nanoparticles with an average diameter of approximately 8.8 nm. Biological evaluation showed that the synthesized C5-AuNPs exerted pronounced dose-dependent cytotoxicity against MCF-7 breast cancer cells with an IC₅₀ threshold of 21.17 ppm, while no toxicity appears in normal HEK293 cells. Mechanistically, the C5-AuNPs induced early apoptosis and inhibit cell-cycle progression at the stage of G0/G1. Collectively, these findings demonstrate that C. vulgaris-mediated AuNPs represent a promising preliminary in vitro findings for cancer therapy candidate.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4035-4053"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148314884","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling Luteolin's Modulatory Effects on α-Synuclein Aggregation: A Combined Biophysical, Computational, and C. elegans Approach.","authors":"Syed Moasfar Ali, Md Nadir Hassan, Nida Zaidi, Muhammad Uzair Ashraf, Mahmood Akbar, Aamir Nazir, Rizwan Hasan Khan","doi":"10.1007/s12013-026-02109-w","DOIUrl":"10.1007/s12013-026-02109-w","url":null,"abstract":"<p><p>Parkinson's disease is associated with amyloid fibrillation of alpha-synuclein (α-syn) that involves the aggregation of protein into insoluble fibrils. In this study, we investigate the effect of luteolin (LUT) on α-syn aggregation in vitro, in silico, and in Caenorhabditis elegans. The decrements in light scattering and ThT fluorescence reveal that there is less formation of α-syn aggregates with an increase in the concentration of LUT. Also, a decrease in the ANS fluorescence depicts less exposure of hydrophobic patches in α-syn in the presence of LUT. Far UV CD data shows that LUT resists conversion structure into beta sheets. Dynamic light scattering and transmission electron microscopy revealed that LUT decreases the size and number of fibrils formed. Additionally, LUT reduced RBC hemolysis, indicating its potential therapeutic value in preventing the formation of toxic aggregates. In vivo studies in C. elegans also show that LUT improves the mitochondrial health, reduces reactive oxygen species (ROS), and enhances worm motility. Computational studies provide probable mechanistic insight that LUT interacted with α-syn via hydrophobic and hydrogen interactions, thus limiting the formation of fibrils by masking the sites that might be involved in aggregation. Consequently, LUT is an inhibitor of α-syn aggregation and may be a potential therapeutic agent against Parkinson's disease.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4147-4160"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148429607","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Vitro and In Silico Assessment of Anticancer Activity of Venom-Derived and Biomimetic Neurotransmitter Inhibitor Pentapeptides.","authors":"Dilan Akhan, Bilge Bicak, Gizem Akman, Serda Kecel Gunduz","doi":"10.1007/s12013-026-02095-z","DOIUrl":"10.1007/s12013-026-02095-z","url":null,"abstract":"<p><p>Cancer, resulting from uncontrolled cell proliferation, is a leading cause of death worldwide and its incidence continues to rise. Challenges such as late diagnosis, aggressive tumor biology, and therapeutic resistance limit the effectiveness of current treatment strategies. Therefore, therapeutic peptides capable of penetrating cells and exhibiting high target specificity have emerged as an innovative approach in cancer therapy. Peptides derived from animal venoms have demonstrated antitumor activity and diagnostic potential in glioma, colon, ovarian, breast, and prostate cancers; for instance, chlorotoxin, crotamine, and eosinophil-derived neurotoxin are being investigated as biomarkers or target-specific anticancer agents. Critical signaling pathways in cancer cells, including PI3K/Akt/mTOR, EGFR, androgen receptor, PSMA, and CDK8, have been explored as targets for peptide-based therapeutics in both in silico and experimental studies. In this study, the anticancer potential of Vialox (Gly-Pro-Arg-Pro-Ala) derived from Tropidolaemus wagleri venom and Leuphasyl (Tyr-Ala-Gly-Phe-Leu) derived from botulinum neurotoxin as a biommimetic peptide were evaluated through molecular docking, MM-GBSA calculations, molecular dynamics simulations, ADMET analysis, and MTT cell assays. Docking studies revealed strong binding affinities of Vialox and Leuphasyl to key oncogenic targets, with docking scores ranging from -10.63 to -7.59 kcal/mol. The highest binding was observed for EGFR-Leuphasyl (-10.63 kcal/mol), followed by Vialox-EGFR (-9.24 kcal/mol). PI3K docking scores were also high, with -9.05 and -9.15 kcal/mol for Vialox and Leuphasyl, respectively. ADMET predictions indicated limited oral bioavailability and minimal BBB penetration. MM-GBSA calculations showed that Vialox and Leuphasyl interact differently with the EGFR binding pocket. Vialox binding is mainly driven by electrostatic interactions, with a binding energy of -65.29 kcal/mol. Leuphasyl relies on hydrophobic interactions but has a lower binding energy of -46.68 kcal/mol due to differences in charge distribution and polar interactions. In vitro MTT assays showed that both peptides exhibited the most pronounced cytotoxic effects in MDA-MB-231 and PC3 cells, supporting their potential selective anticancer activity. The findings suggest that both peptides may exhibit selective anticancer effects, and that combining in silico approaches with experimental validation represents an effective strategy for developing novel peptide-based cancer therapeutics.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"3909-3950"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148395335","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Rosemary (Rosmarinus officinalis) Extract Attenuates Methotrexate-Induced Oxidative Stress, Inflammatory Disturbance, and Apoptotic Lung Injury: A Molecular and Histopathological Study.","authors":"Abd El Ghany A Moustafa, Aly Fahmy Mohamed, Ibrahim M Ibrahim Amr, Hassan Fathy Kaabo, Laila El-Sayed El-Moselhy, Tamer Albasyoni Gomah, Amer M Abdel Hamid, Amal Mahmoud Hammad, Walid Mostafa Sayed Ahmed, Fawzy Mahmoud Abou Galala, Fayez Mohammed Abd Elfattah Elbayoumy, Mohamed E Amer, Mohammed E Elmitwalli, Hagar Wahdan Elazab, Ahmed E El Nagar, Khaled Mohamed Elsadek, Ahmed Nahed Zaky Mohammed, Suhaib Naeem, Mohamad A Soliman, Mohammed Ali A Majrashi, Rasha G Elsafty, Heba M K Youssef","doi":"10.1007/s12013-026-02128-7","DOIUrl":"10.1007/s12013-026-02128-7","url":null,"abstract":"<p><p>Methotrexate (MTX) is widely used as a chemotherapeutic and immunosuppressive agent, but its clinical use is limited by toxic effects associated with oxidative stress, inflammatory dysregulation, and apoptosis. Rosmarinus officinalis (rosemary) extract has documented antioxidant, anti-inflammatory, and cytoprotective properties. This study aimed to evaluate the protective effects of rosemary extract against MTX-induced lung injury through biochemical, histopathological, immunohistochemical, and molecular analyses. A total of 40 adult male Wistar rats were randomly allocated to four experimental groups (n = 10/group): Control, Rosemary, MTX, and Rosemary + MTX. Lung tissues were assessed for oxidative stress markers, including reactive oxygen species (ROS), malondialdehyde (MDA), and reduced glutathione (GSH); inflammatory cytokines (IL-6 and TNF-α); histopathological and immunohistochemical changes; and expression of apoptosis-related markers (p53 and Bcl-2). MTX significantly increased ROS, MDA, TNF-α, p53 gene expression, p53 immunoreactivity, and collagen deposition, while it decreased GSH, IL-6, and Bcl-2 expression compared with the control group (P < 0.05). Co-treatment with rosemary attenuated MTX-induced increases in ROS, MDA, TNF-α, p53 expression, collagen deposition, and histopathological injury, and partially restored GSH and Bcl-2 levels compared with the MTX group (P < 0.05). Compared with the control group, several measured parameters remained significantly altered in the Rosemary + MTX group according to Tukey post-hoc comparisons, indicating partial rather than complete recovery. Rosemary extract partially attenuated MTX-associated oxidative, cytokine, apoptosis-related, and histopathological alterations in rat lung tissue. Further mechanistic and translational studies are required.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4381-4394"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148811812","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Asparagus Polysaccharide Ameliorates TNF-α-Mediated MH7A Cell Dysfunction and Collagen-Induced Arthritis in Mice.","authors":"Mingfeng Yang, Fangyan Xu, Bin Zhang, Lijun Xu","doi":"10.1007/s12013-026-02136-7","DOIUrl":"10.1007/s12013-026-02136-7","url":null,"abstract":"<p><p>Although Rheumatoid arthritis (RA) management has improved substantially with modern pharmacological therapies, many patients still fail to achieve sustained treatment targets. This study investigated the therapeutic effects and potential mechanisms of asparagus polysaccharide (APS) in tumor necrosis factor-α (TNF-α)-induced MH7A cell dysfunction and collagen-induced arthritis (CIA) in mice. In vitro, MH7A cells were treated with different concentrations of APS, and cell viability, apoptosis, cell cycle distribution, and related protein and gene expression were assessed. In vivo, CIA model was established in mice, followed by APS administration. Paw volume, joint histopathology, serum cytokine levels, and the splenic Th17 cell proportion were evaluated. APS (≥ 3 mg/mL, 48 h) decreased MH7A cell viability. APS (3, 4, 5 mg/mL, 24 h) promoted apoptosis and inhibited proliferation in TNF-α-treated MH7A cells. APS (5 mg/mL, 24 h) downregulated Bcl-2 and cell cycle-related genes and suppressed NF-κB pathway activation. In the CIA mouse model, APS reduced paw volume, alleviated joint inflammation and tissue damage, decreased serum TNF-α, interleukin-6 (IL-6), IL-17A levels, increased IL-10 levels, and inhibited splenic Th17 cell expansion in a dose-dependent manner, without a significant effect on Treg cell proportion. APS exerts anti-arthritic effects in vitro and in vivo by regulating synoviocyte proliferation and apoptosis, inflammatory cytokine balance, and Th17 cell differentiation. The in vitro mechanism was associated with inhibition of NF-κB signaling. These findings provide preliminary experimental evidence supporting further investigation of APS as a candidate therapeutic agent for RA.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4449-4459"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148760087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Retraction Note: Exosome-mediated Transfer of lncRNA in Liver Associated Diseases; Uncovered Truths.","authors":"Raed Obaid Saleh, Hamad Ali Hamad, Maryam Abdulrahman Najim, Soumya V Menon, Mandeep Kaur, G V Sivaprasad, Mohammad Abohassan, Wen-Tau Juan, Beneen Husseen, Yasser Fakri Mustafa","doi":"10.1007/s12013-026-02059-3","DOIUrl":"10.1007/s12013-026-02059-3","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4687"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147479396","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Selective Cytotoxic and Redox-Associated Effects of Plant-Derived Terpenoids in Human Glioblastoma Cells.","authors":"Abdul Saltuk Buğra Daş, Mehmet Enes Arslan, Murat Aydemir, Abdurrahim Kadı, Taha Aykılıç, Berk Can Köksal, Hasan Türkez","doi":"10.1007/s12013-026-02122-z","DOIUrl":"10.1007/s12013-026-02122-z","url":null,"abstract":"<p><p>Glioblastoma (GBM) is the most common and aggressive primary malignant brain tumor and remains highly resistant to standard therapies. Because redox homeostasis is frequently altered in GBM, small natural products that affect viability together with oxidative-stress endpoints may provide promising leads for further development. Here, we evaluated the terpenoids α-pinene, farnesene, carvone, and terpinolene for cytotoxicity and selectivity in human glioblastoma (U87MG) cells compared with primary human dermal fibroblasts (HDFa). Cell viability was assessed by the MTT assay, and nuclear morphology was evaluated using Hoechst 33,258 staining. Apoptosis-related effects were examined by measuring caspase-3 activity, and oxidative balance was assessed using total antioxidant status (TAS) and total oxidant status (TOS) assays. The terpenoids reduced U87MG viability in a concentration-dependent manner while showing substantially lower toxicity in HDFa cells. At 30 µg/mL, all tested terpenoids increased caspase-3 activity in U87MG cells and were associated with increased TAS and decreased TOS relative to control. Overall, these findings identify these terpenoids as selective in vitro hits against U87MG cells and support follow-up studies incorporating direct ROS profiling and expanded apoptosis assays to clarify mechanism.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4331-4346"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148719459","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Protective Effect of Astragalus Polysaccharides Against Cisplatin‑Induced Pulmonary Epithelial Cell Injury: An Integrated Network Toxicology and In Vitro Validation.","authors":"Yan Liu, Liyan Ma, Lei Zhang, Liangding Dou, Ninghui Yang, Junrong Li, Kun Zhang","doi":"10.1007/s12013-026-02119-8","DOIUrl":"10.1007/s12013-026-02119-8","url":null,"abstract":"<p><p>Cisplatin (CDDP) is a widely used anticancer drug that generates significant oxidative stress, resulting in lung inflammation and injury. Currently, there is limited research on the related mechanisms and strategies. Astragalus polysaccharides (APS) are the main bioactive molecules in Astragalus that possess immunomodulatory, anti-inflammatory and antioxidant activities, and are clinically used for preventing pulmonary fibrosis and treating lung inflammation. Therefore, the present study aimed to investigate the protective effects of APS on CDDP-induced lung epithelial cell injury using network toxicology and network pharmacology to predict the key pathways involved in CDDP-induced injury and APS-mediated protection, and validating the predictions using in vitro assays. Our results demonstrated that APS could significantly reduce the death rate of Beas-2B cells and attenuated the oxidative stress caused by CDDP. The mitochondrial function was enhanced, mitochondrial reactive oxygen species (ROS) was suppressed, malondialdehyde (MDA) content and ferrous iron were decreased, thereby inhibiting ferroptosis and alleviating lung cell injury induced by CDDP. Therefore, the present study demonstrated that APS may attenuate CDDP-induced oxidative stress and iron overload, thereby inhibiting ferroptosis and alleviating lung cell injury. The findings of the present study may provide a potential clinical application.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4287-4298"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148704856","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Digoxin Derivative BD-8 Attenuates Oxidative Stress and Neuroinflammation in an LPS-Induced Experimental Model.","authors":"Vitória Karolina Brandão Souza, Davi Azevedo Ferreira, Antônio Pereira Ribeiro Arantes, Elton de Sá Figueiredo, Millena Ferreira Rodrigues Fonseca, Pâmela Yasmin de Oliveira Ferreira, Martina Raissa Ribeiro, Paulo César Ghedini, Andreza Amália de Freitas Ribeiro, José Augusto Ferreira Perez Villar, Maira de Castro Lima, Israel José Pereira Garcia, Hérica L Santos, Cristoforo Scavone, Sandra Rodrigues Mascarenhas, Jacqueline Alves Leite","doi":"10.1007/s12013-026-02108-x","DOIUrl":"10.1007/s12013-026-02108-x","url":null,"abstract":"<p><p>Chronic neuroinflammation contributes to neuronal damage through oxidative stress and inflammatory mediators, highlighting the need for novel therapeutic strategies. This study evaluated the cytotoxicity and neuroprotective effects of BD-8, a digoxin-derived cardiosteroid, in vitro and in vivo. BD-8 showed lower cytotoxicity than digoxin, with CC₅₀ values of 7.4 µM in SH-SY5Y cells and 8.5 µM in BV-2 cells, while digoxin presented a CC₅₀ of 33 nM in SH-SY5Y cells. In vivo, BD-8 did not alter cardiac, hepatic, or renal biochemical markers, supporting its systemic safety profile. In the open-field test, BD-8 prevented LPS-induced locomotor impairment, significantly increasing total crossings at 0.56 mg/kg (62.07 ± 3.269; P < 0.05) and 1.12 mg/kg (66.31 ± 5.282; P < 0.001) compared to the LPS group. BD-8 also reduced lipid peroxidation levels in the cortex at 0.56 mg/kg (14.38 ± 0.5568; P < 0.001) and 1.12 mg/kg (13.94 ± 0.8372; P < 0.001), and in the hippocampus at 1.12 mg/kg (14.04 ± 0.5223; P < 0.001). Additionally, BD-8 modulated antioxidant enzyme activities, superoxide dismutase (SOD), catalase (CAT), glutathione peroxidase (GPx), in both the cortex and hippocampus and reduced IL-6 levels in these brain regions without altering BDNF (Brain Derived Neurotrophic Factor) levels. These findings support BD-8 as a promising candidate for neuroinflammatory disorders.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":"4131-4145"},"PeriodicalIF":3.0,"publicationDate":"2026-09-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC13586031/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148314875","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}