Cell Biochemistry and Biophysics最新文献

{"title":"Reversible Myeloperoxidase Inhibition by Benzodioxole Carboxamides: Insights from In Silico and InVitro Studies.","authors":"Reshma Rajan, Sambantham Karthikeyan, Rajagopal Desikan","doi":"10.1007/s12013-025-01880-6","DOIUrl":"https://doi.org/10.1007/s12013-025-01880-6","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038863","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Boron Containing Curcumin-Like Compound as an Acetylcholinesterase Inhibitor and Anticancer Agent: Synthesis, Biological Evaluation, and Computational Insights.","authors":"Ferah Comert Onder, Kadircan Ural, Alper Onder, Bulent Ozpolat, Mehmet Ay","doi":"10.1007/s12013-025-01885-1","DOIUrl":"https://doi.org/10.1007/s12013-025-01885-1","url":null,"abstract":"<p><p>Alzheimer's disease (AD) and cancer are significant global health challenges that highlight the need for the development of new therapeutics. Targeting biological mechanisms involved in both AD and cancer could be an effective treatment strategy for developing novel inhibitors. In this study, we investigated the effect of a newly synthesized boron containing curcumin-like compound as a potential acetylcholinesterase (AChE) inhibitor along with its cytotoxic effects on breast and colorectal cancer cell lines. Compound A exhibited a potent AChE inhibitory activity (IC₅₀ = 22.89 ± 2.32 nM), demonstrating that it was more effective than the known inhibitors donepezil (IC₅₀ = 28.32 ± 3.27 nM) and tacrine. Compound A showed a moderate cytotoxic activity on MCF-7 and BT20 cells with the IC₅₀ values 40.70 ± 2.31 μM and 41.71 ± 4.51 μM, respectively. Throughout molecular dynamics (MD) simulations, the RMSD value of the protein was calculated as 1.56 ± 0.20 Å and 1.65 ± 0.19 Å for the complexes of compound A and curcumin, respectively. The interactions with specific amino acid residues such as Tyr124, Tyr337, and Trp86 for AChE, and Trp82, His438, and Tyr332 for BChE were obtained. Additionally, MM/GBSA calculations demonstrated that Compound A had the highest binding free energies (-88.89 ± 8.34 kcal/mol for AChE and -73.25 ± 8.83 kcal/mol for BChE) compared to curcumin (-67.87 ± 5.48 kcal/mol for AChE and -51.68 ± 5.28 kcal/mol for BChE) and tacrine (-56.67 ± 2.22 kcal/mol for BChE) were calculated. Overall, these findings suggest that Compound A is a promising agent with its potent AChE inhibitory activity and anticancer potential, making it a valuable candidate for further research in neurodegenerative diseases and cancer therapy.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038887","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Green Synthesized Silver Nanoparticles from Meyna Laxiflora: Binding with HSA and Anti-biofilm Potential.","authors":"Samaresh Paria, Basudev Shit, Somenath Kundu, Subrata Munan, Subhamoy Dey, Subhajit Barman, Aziza Nasrin, Chandradipa Ghosh, Maidul Hossain","doi":"10.1007/s12013-025-01893-1","DOIUrl":"https://doi.org/10.1007/s12013-025-01893-1","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-12","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145038865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Anti-inflammatory Effects of Synaptamide in the Peripheral Immune System in a Murine Model of Traumatic Brain Injury.","authors":"Igor Manzhulo, Arina Ponomarenko, Olga Manzhulo, Darya Ivashkevich","doi":"10.1007/s12013-025-01890-4","DOIUrl":"10.1007/s12013-025-01890-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028703","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Unraveling the Systems Biology of Curcumin: A Mini-review of its Anti-diabetic Potential through Network Pharmacology.","authors":"Miah Roney, Mohd Fadhlizil Fasihi Mohd Aluwi","doi":"10.1007/s12013-025-01888-y","DOIUrl":"https://doi.org/10.1007/s12013-025-01888-y","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145028725","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Melatonin Ameliorates Isoproterenol-Induced Cardiac Fibrosis by Suppressing BIP/PERK/CHOP Signaling Pathways; Insights from In Silico and In vivo Studies.","authors":"Farkhondeh Pooresmaeil, Reza Jafari-Shakib, Behnam Hasannejad-Asl, Farhad Mashayekhi, Mojtaba Hedayati Ch, SeyyedMohammadTaghi Razavi-Toosi","doi":"10.1007/s12013-025-01847-7","DOIUrl":"https://doi.org/10.1007/s12013-025-01847-7","url":null,"abstract":"<p><p>In cardiovascular research, melatonin has shown promise in exhibiting antifibrotic properties and modulating endoplasmic reticulum (ER) stress. However, the exact mechanism by which it influences myocardial fibrosis has not been fully clarified. Therefore, this research aimed to investigate the inhibitory effect of melatonin on the progression of myocardial fibrosis through a mechanism involving the BIP/PERK/CHOP signaling pathway, both in silico and in vivo experimental models. In in silico studies, molecular docking and molecular dynamics simulations were employed to predict the binding affinity of melatonin to ER stress arm proteins, BIP, and PERK. Following, in vivo experiments were carried out to confirm in silico analyses. In animal studies, rats were administered melatonin intraperitoneal (10 mg/kg per day) for 3 weeks, and on the 6th and 7th days, they were given isoproterenol at a dose of 170 mg/kg subcutaneous to estabilish myocardial fibrosis model. The morphological changes in cardiac tissue were assessed using hematoxylin and eosin (H&E) and Masson's trichrome staining. Additionally, the expression of BIP and CHOP, a key downstream target of the PERK pathway, was analyzed through real-time PCR and immunohistochemistry. In silico studies suggest melatonin interacts with BIP and PERK, demonstrating strong binding energy and forming a stable complex with both proteins. However, its affinity and stability with PERK are greater than with BIP. Furthermore, immunohistochemistry and qRT-PCR findings indicated that melatonin notably downregulated the expression of BIP and CHOP in the isoproterenol-induced cardiac fibrosis model. The strong binding affinity of melatonin for BIP and PERK, coupled with its impact on the downregulation of BIP and CHOP proteins in the isoproterenol-induced cardiac fibrosis model, suggests that melatonin's antifibrotic effects on myocardial tissue may be related to its ER stress inhibitory effects.</p>","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Integrated In Vivo, In Vitro, and In Silico Evaluation of Spermacoce ocymoides Methanolic Extract for Antidiarrheal, Thrombolytic, and Antiarthritic Activities.","authors":"Bakul Akter, Kutub Uddin Ahamed, Md Hossain Sohrab, Silvia Aishee, Bidhan Sarkar, Afra Anika, Mohammed Kamrul Hossain, Hanan M Alharbi, Emad Rashad Sindi, Most Nazmin Aktar","doi":"10.1007/s12013-025-01884-2","DOIUrl":"https://doi.org/10.1007/s12013-025-01884-2","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022531","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Immunostimulatory and Immunomodulatory Effects of Vitamin B12 Derivatives on Macrophages Through the Modulation of JNK Pathway.","authors":"Ömer Mete Başkan, Semanur Ercan, Ece Aydın, Yunus Bora Subaşı, Esra Aydemir, Furkan Ayaz","doi":"10.1007/s12013-025-01883-3","DOIUrl":"https://doi.org/10.1007/s12013-025-01883-3","url":null,"abstract":"&lt;p&gt;&lt;p&gt;Vitamin B12 is a vital water-soluble vitamin containing a central cobalt atom within its corrin ring structure. It exists in several derivatives, among which methylcobalamin (MeCbl) and adenosylcobalamin (AdCbl) are the biologically active forms that serve as cofactors in essential enzymatic reactions. Although the neurological and hematological consequences of vitamin B12 deficiency have been extensively studied, its role in immune regulation remains less well understood. Considering that macrophages are key effector cells of innate immunity, this study aimed to investigate the immunostimulatory and immunomodulatory properties of MeCbl and AdCbl on murine macrophages (J774.2 cell line) with a particular focus on cytokine responses and the JNK signaling pathway. Macrophages were cultured under controlled conditions and treated with non-cytotoxic concentrations of MeCbl and AdCbl (1, 5, and 10 µg/mL), either alone or in combination with lipopolysaccharide (LPS, 1 µg/mL) as a pro-inflammatory stimulus. Cell viability was first confirmed using Trypan Blue exclusion to ensure that the tested concentrations did not impair cellular survival. Supernatants were then analyzed for cytokine production (TNF-α, IL-6, IL-12p40, GM-CSF) using ELISA, and intracellular signaling was investigated through flow cytometric analysis of phosphorylated JNK (p-JNK) levels. The results revealed that under non-inflammatory conditions, neither MeCbl nor AdCbl significantly altered cytokine production, suggesting a lack of direct immunostimulatory activity in resting macrophages. However, in the presence of LPS, both derivatives markedly increased TNF-α levels, consistent with enhanced pro-inflammatory activation. At the same time, both compounds significantly suppressed IL-6 and IL-12p40 production, pointing to their capacity to counterbalance excessive Th1-driven responses. Importantly, AdCbl demonstrated a unique, concentration-dependent reduction in GM-CSF levels, whereas MeCbl induced only minimal and inconsistent changes, indicating that AdCbl exerts a more selective regulatory profile while MeCbl has broader but less concentration-dependent effects. Flow cytometry further confirmed that both derivatives strongly enhanced JNK phosphorylation, implicating this signaling pathway as a mechanistic link between vitamin B12 derivatives and cytokine modulation. Taken together, these findings demonstrate that MeCbl and AdCbl exert distinct yet overlapping immunomodulatory effects in macrophages. By simultaneously promoting TNF-α while limiting IL-6, IL-12p40, and GM-CSF (for AdCbl), these compounds display a complex balance of pro- and anti-inflammatory activities mediated, at least in part, through the JNK pathway. This dual role highlights their potential relevance in fine-tuning immune responses, preventing excessive inflammation, and supporting host defense. The study provides new insights into the immunological functions of vitamin B12 derivatives and suggests that ","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"In Silico Identification of Natural SIRT1 Inhibitors through Molecular Docking, Dynamics Simulation, and MM/PBSA.","authors":"Shefali Kardam, Pravir Kumar","doi":"10.1007/s12013-025-01886-0","DOIUrl":"https://doi.org/10.1007/s12013-025-01886-0","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145022561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Correction: Blocking GATA6 Alleviates Pyroptosis and Inhibits Abdominal Wall Endometriosis Lesion Growth Through Inactivating the PI3K/AKT Pathway.","authors":"Xiufang Du, Hongjie Yang, Xiaobei Kang, Changna Fu, Tao Yang","doi":"10.1007/s12013-025-01882-4","DOIUrl":"https://doi.org/10.1007/s12013-025-01882-4","url":null,"abstract":"","PeriodicalId":510,"journal":{"name":"Cell Biochemistry and Biophysics","volume":" ","pages":""},"PeriodicalIF":2.5,"publicationDate":"2025-09-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144999348","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}