European Journal of Histochemistry最新文献_第2页

Independent and interactive roles of hirudin and HMGB1 interference in protecting renal function by regulating autophagy, apoptosis, and kidney injury in chronic kidney disease. 水蛭素和 HMGB1 通过调节慢性肾病患者的自噬、细胞凋亡和肾损伤，在保护肾功能方面发挥独立和交互作用。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-04-07 DOI: 10.4081/ejh.2025.4182

Ying Li, Xuan Gao, Yao Chen, Huihui Li, Jing Tang, Wei Sun

{"title":"Independent and interactive roles of hirudin and HMGB1 interference in protecting renal function by regulating autophagy, apoptosis, and kidney injury in chronic kidney disease.","authors":"Ying Li, Xuan Gao, Yao Chen, Huihui Li, Jing Tang, Wei Sun","doi":"10.4081/ejh.2025.4182","DOIUrl":"10.4081/ejh.2025.4182","url":null,"abstract":"Chronic kidney disease (CKD) is a progressive disorder characterized by renal fibrosis, inflammation, and dysregulated autophagy and apoptosis. High-mobility group box 1 (HMGB1) plays a crucial role in regulating autophagy in CKD. Hirudin, a potent thrombin inhibitor, has demonstrated antifibrotic and anti-inflammatory properties, but its effects on autophagy and apoptosis in CKD remain unclear. In this study, a rat model of renal interstitial fibrosis (RIF) and an HK-2 cell culture model were established to assess the effects of varying doses of hirudin and HMGB1 interference. Molecular and histological analyses, including RTqPCR, Western blot, TUNEL staining, hematoxylin-eosin (H&E) staining, immunofluorescence, and immunohistochemistry (IHC), were performed to assess renal injury, fibrosis, apoptosis, and autophagy-related markers. Hirudin treatment significantly reduced the expression of LC3, ATG12, ATG5, α-SMA, COL1A1, caspase-3, and caspase-9 while increasing P62 levels (p<0.05). It also lowered the renal coefficient (p<0.001) and apoptosis levels. The optimal effective concentration of hirudin in vitro was determined to be 4.8 ATU/mL (p<0.001). HMGB1 interference suppressed autophagy and apoptosis, as indicated by decreased LC3-II/LC3-I, ATG12, ATG5, caspase-3, and caspase-9 levels, increased P62 expression (p<0.001), and reduced apoptosis. However, simultaneous HMGB1 interference in hirudin-treated cells weakened the therapeutic effects of hirudin, leading to increased autophagy and apoptosis markers, decreased P62 levels, and a higher renal coefficient. These findings indicate that hirudin exerts protective effects in CKD by modulating autophagy and apoptosis, potentially through HMGB1 regulation. These findings highlight the therapeutic potential of targeting these mechanisms in renal dysfunction and underscore the necessity for further research to support clinical applications.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12038336/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796691","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum - Effect of CDX2 on proliferation, invasion, migration, and apoptosis of duodenal cancer cells. CDX2对十二指肠癌细胞增殖、侵袭、迁移和凋亡的影响。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-04-07 Epub Date: 2025-05-20 DOI: 10.4081/ejh.2025.4235

Jun Pan, Yi Zhao, Yu Zhang, Yuhe Zhou, Fengxuan Zhang, Yitian Chen, Xiaoyuan Chu

引用次数: 0

Effect of CDX2 on proliferation, invasion, migration, and apoptosis of duodenal cancer cells. CDX2对十二指肠癌细胞增殖、侵袭、迁移及凋亡的影响。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-04-07 Epub Date: 2025-04-24 DOI: 10.4081/ejh.2025.4203

Jun Pan, Yi Zhao, Yu Zhang, Yuhe Zhou, Fengxuan Zhang, Yitian Chen, Xiaoyuan Chu

{"title":"Effect of CDX2 on proliferation, invasion, migration, and apoptosis of duodenal cancer cells.","authors":"Jun Pan, Yi Zhao, Yu Zhang, Yuhe Zhou, Fengxuan Zhang, Yitian Chen, Xiaoyuan Chu","doi":"10.4081/ejh.2025.4203","DOIUrl":"10.4081/ejh.2025.4203","url":null,"abstract":"This study investigates the expression and biological role of caudal homologous transcription factor 2 (CDX2) in duodenal carcinoma. Paraffin-embedded samples from 40 duodenal carcinoma cases were analyzed using immunohistochemistry on a tissue microarray to assess CDX2 expression and its prognostic significance. CDX2 overexpression plasmids and CDX2-siRNA were transfected into colon and duodenal cancer cells. Transfection efficiency was confirmed by RT-PCR and Western blotting. Cell proliferation was assessed using CCK8 assay, migration via scratch assay, and cell cycle and apoptosis by flow cytometry. CDX2 staining was primarily nuclear, with a positive rate of 65% in duodenal carcinoma tissues, significantly lower than in adjacent non-tumor tissues (p<0.05). CDX2-positive patients had better prognoses than negative patients (p<0.05). Reduced CDX2 expression significantly enhanced the proliferation of CaCO2 and HuTu-80 cells (p<0.001), whereas CDX2 overexpression suppressed proliferation (p<0.001). CDX2 knockdown increased migration, while its overexpression reduced migration (p<0.05). CDX2 overexpression led to a significant increase in G0/G1 phase cells and a decrease in S phase cells (p<0.05), whereas knockdown reduced G0/G1 phase cells and increased S and G2/M phase cells (p<0.05). Apoptosis was significantly increased following CDX2 overexpression (p<0.001) and decreased after CDX2 knockdown (p<0.001). CDX2 expression is downregulated or lost in duodenal carcinoma, acting as a tumor suppressor. CDX2 may serve as a crucial biomarker for predicting the onset, progression, and treatment of duodenal carcinoma.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12067183/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144040018","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups. 探讨AMACR、ERG和AR免疫染色在前列腺腺癌中的疗效及其与新型分级组的关系。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-02-11 DOI: 10.4081/ejh.2025.4172

Mohamed O Andarawi, Hassan Otifi, Hesham Hassan, Adil A Yousif, Saadalnour A Mustafa, Shawgi A Elsiddig, Asad Ma Babker, Elryah I Ali, Omer Osman Elhag

{"title":"Exploring the efficacy of AMACR, ERG, and AR immunostains in prostatic adenocarcinoma and their association with novel grade groups.","authors":"Mohamed O Andarawi, Hassan Otifi, Hesham Hassan, Adil A Yousif, Saadalnour A Mustafa, Shawgi A Elsiddig, Asad Ma Babker, Elryah I Ali, Omer Osman Elhag","doi":"10.4081/ejh.2025.4172","DOIUrl":"10.4081/ejh.2025.4172","url":null,"abstract":"The study examines the utility of AMACR, ERG, and AR immunostains in diagnosing prostatic adenocarcinoma (PCa) and assessing prognosis in comparison to the Gleason score and new WHO grading groups. Seventeen PCa biopsies and five benign prostatic hyperplasia (BPH) biopsies were analyzed. Immunoreactivity, scored from 1 to 3 based on percentage of positive cells and intensity of expression, was assessed, revealing 76.47% positivity for AMACR, 35.29% for ERG, and 94.12% for AR in PCa cases, with variable scores and intensity among markers and grade groups. AMACR sensitivity and ERG specificity were noted. Higher-grade PCa exhibited increased positivity for both markers, indicating prognostic significance. In BPH cases, AMACR showed positivity in 2 cases, ERG in 1, and AR in all cases, albeit with lower expression. Differential expression was observed among immunomarkers and grade groups of malignancy. AMACR and ERG stains serve as sensitive and specific markers for PCa diagnosis and prognosis. Their increasing positivity with higher-grade groups underscores prognostic value. These findings highlight the importance of immunostains in refining PCa diagnosis and prognostication.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-02-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11864098/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143392401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of S100β during mouse cochlear development. 小鼠耳蜗发育过程中S100β的表达。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 Epub Date: 2025-03-10 DOI: 10.4081/ejh.2025.4189

Wenjing Liu, Yongchun Zhang, Cheng Liang, Lizhong Su

{"title":"Expression of S100β during mouse cochlear development.","authors":"Wenjing Liu, Yongchun Zhang, Cheng Liang, Lizhong Su","doi":"10.4081/ejh.2025.4189","DOIUrl":"10.4081/ejh.2025.4189","url":null,"abstract":"In the present study, the expression of S100β was examined in the mouse cochlea from embryonic day 17 (E17) to postnatal day 32 (P32) using immunofluorescence, aiming to explore its possible role in auditory system. At E17, S100β expression was not detected, except in the external cochlear wall. Starting at E18.5, S100β staining appeared in the organ of Corti and the stria vascularis. In the E18.5 and P1 organ of Corti, S100β was confined to the developing pillar cells. By P6, cytoplasmic staining of S100β was evident in the inner and outer pillar cells, forming the tunnel of Corti. Additionally, S100β expression extended medially into the three rows of Deiter's cells, with labeling of their phalangeal processes. At P8, S100β continued to be expressed in the heads, bodies, and feet of the two pillar cells, as well as in the soma and phalangeal processes of the three rows of Deiter's cells. In the lateral wall of the P8 cochlea, S100β was expressed not only in the stria vascularis but also in the spiral ligament. Between P10 and P12, S100β expression was maintained in the Deiter's cells and pillar cells of the organ of Corti, as well as in the lateral wall, and spiral limbus. From P14 onwards, S100β expression ceased in the stria vascularis, though it persisted in the spiral ligament and spiral limbus into adulthood. Within the P14 and P21 organ of Corti, S100β remained in the Deiter's and pillar cells. S100β immunostaining was not observed in the phalangeal processes of Deiter's cells but was specifically present in the Deiter's cell cups at P21. In the adult cochlea (P28 and P32), S100β expression declined in both Deiter's and pillar cells. The dynamic spatiotemporal changes in S100β expression during cochlear ontogeny suggest its role in cochlear development and hearing function.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956554/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607051","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Restorative effects of camellia oil on the skin-barrier function in a model of DNCB-induced atopic dermatitis. 茶油对dncb诱导的特应性皮炎模型皮肤屏障功能的恢复作用。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 DOI: 10.4081/ejh.2025.4147

Shicheng Jiao, Lijun Deng, Mu Niu, Jie Yang

{"title":"Restorative effects of camellia oil on the skin-barrier function in a model of DNCB-induced atopic dermatitis.","authors":"Shicheng Jiao, Lijun Deng, Mu Niu, Jie Yang","doi":"10.4081/ejh.2025.4147","DOIUrl":"10.4081/ejh.2025.4147","url":null,"abstract":"This study aimed to evaluate the therapeutic efficacy of camellia oil on 2,4-dinitrochlorobenzene (DNCB)-induced atopic dermatitis (AD) in mice, as well as its effect on the expression of skin-barrier-related proteins. A mouse model of AD was created via topical application of DNCB; subsequently, the animals were randomly divided into four groups: the blank control (Control), model (Model), moisturizing cream (Moisturizer), and camellia oil (Camellia) groups. The Camellia group received camellia oil, whereas the Moisturizer group was treated with moisturizing cream, as a positive control. Skin lesions, ear and back tissue morphology, and the serum levels of IgE, IL-4, and IFN-γ were analyzed. Compared with the Control group, AD mice exhibited erythema, papules, dryness, peeling, and significantly higher serum IgE and IL-4 levels. Compared with the Model group, treatment with camellia oil and moisturizing cream considerably reduced skin inflammation, ear thickness, and scratching frequency. A histopathological analysis revealed that camellia oil reduced inflammatory-cell infiltration and edema in the AD-affected skin. Furthermore, camellia oil upregulated filaggrin (FLG), thus aiding in skin-barrier repair. These findings suggest that camellia oil significantly improves AD symptoms, enhances FLG expression, and restores the damaged skin barrier in AD mouse models.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11788714/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143015603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reliable hexokinase 3 protein detection in human cell lines and primary tissue. 人细胞系和原代组织中己糖激酶3蛋白的可靠检测。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 Epub Date: 2025-03-12 DOI: 10.4081/ejh.2025.4175

Yasmeen H Mady, Carmen G Kalbermatter, Maarij Khan, Anna M Schläfli, Rina Mehmeti, Inti Zlobec, Lucine Christe, Mario P Tschan

{"title":"Reliable hexokinase 3 protein detection in human cell lines and primary tissue.","authors":"Yasmeen H Mady, Carmen G Kalbermatter, Maarij Khan, Anna M Schläfli, Rina Mehmeti, Inti Zlobec, Lucine Christe, Mario P Tschan","doi":"10.4081/ejh.2025.4175","DOIUrl":"10.4081/ejh.2025.4175","url":null,"abstract":"Accurate differentiation of homologous proteins that share high sequence identity remains a significant challenge in biomedical research, as conventional antibodies often lack sufficient specificity, leading to potential misinterpretations. This issue is particularly evident in the study of hexokinases, a family of isoenzymes that catalyze the first step of glycolysis by phosphorylating glucose. Beyond their canonical metabolic roles, hexokinases play critical non-glycolytic functions, especially in cancer biology. However, their unique tissue distributions and context-dependent roles are often obscured by the overlapping specificities of commercially available antibodies, which can produce misleading results. In this study, we rigorously evaluated a panel of antibodies targeting hexokinase isoenzyme 3 (HK3), highlighting the widespread issue of cross-reactivity and insufficient validation. Through this process, we identified and validated a highly specific antibody for HK3, demonstrating its reliability in western blot and immunohistochemistry applications. Using this validated tool, we reveal the distinct localization of HK3 in myeloid cell populations, providing new insights into its potential functional roles in these cells. This work addresses a critical gap in antibody specificity and establishes HK3 as a uniquely expressed gene in myeloid and immune cells and is absent in other cell types under basal conditions. Providing a foundation for future investigations into its context-dependent functions.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11956552/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143607054","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Co-expression of MyHC-15 with other known isoforms in rat muscle spindles. MyHC-15与其他已知亚型在大鼠肌纺锤体中的共表达。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 Epub Date: 2025-03-24 DOI: 10.4081/ejh.2025.4192

Vika Smerdu, Chiedozie Kenneth Ugwoke, Žiga Šink

{"title":"Co-expression of MyHC-15 with other known isoforms in rat muscle spindles.","authors":"Vika Smerdu, Chiedozie Kenneth Ugwoke, Žiga Šink","doi":"10.4081/ejh.2025.4192","DOIUrl":"10.4081/ejh.2025.4192","url":null,"abstract":"Muscle spindles are skeletal muscle sensory receptors composed of intrafusal fibres, partially encapsulated by connective tissue capsule. This capsule encloses the central A and B regions while leaving the distal C region extracapsular. Several past studies in rat have shown that muscle spindles typically contain a single bag1 fibre, a single bag2 fibre, and two smaller chain fibres. Intrafusal fibres co-express multiple myosin heavy chain (MyHC) isoforms: -slow or -1, -slow-tonic, -α, -2a, -2b, -embryonic, and -neonatal. While MyHC-2x was previously thought absent, the recently discovered MyHC-15 isoform has been identified in the C region of rat bag fibres. Using antibodies specific for nine MyHC isoforms and analyzing four different rat skeletal muscles-soleus, extensor digitorum longus, and the lateral and medial heads of gastrocnemius-we aimed to further characterize the co-expression pattern of MyHC-15 with other known isoforms and to determine whether MyHC-2x is expressed in rat intrafusal fibres. While rodents are widely used as animal models in skeletal muscle research, notable species-specific differences in MyHC isoform expression exist. Our findings revealed that MyHC-15 expression in rat intrafusal fibres is less abundant than in human fibres. MyHC-15 was primarily observed in bag fibres but was not detected in the C region, contrary to previous reports in both rat and human. We confirmed the absence of MyHC-2x in rat intrafusal fibres. Similarly, MyHC-embryonic and -neonatal were not detected in the analyzed spindles, suggesting that previously used antibodies may have cross-reacted with MyHC-2a and -2b. While our results partially corroborate previous extensive studies, discrepancies suggest that MyHC expression in intrafusal fibres varies not only along the fibre length but also across muscles.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11983093/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143694326","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum - Effect of Danggui Buxue decoction on hypoxia-induced injury of retinal Müller cells in vitro. 当归补血汤对体外缺氧性视网膜网膜细胞损伤的影响。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 DOI: 10.4081/ejh.2025.4181

Xilin Ge, Caoxin Huang, Wenting Chen, Chen Yang, Wenfang Huang, Jia Li, Shuyu Yang

引用次数: 0

Immunohistochemistry of carbonic anhydrases I, II and VI in the rat lingual serous salivary glands of von Ebner. 大鼠舌腺黏液性唾液腺碳酸酐酶ⅰ、ⅱ、ⅵ的免疫组化。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-01-21 Epub Date: 2025-03-04 DOI: 10.4081/ejh.2025.4159

Robert S Redman

{"title":"Immunohistochemistry of carbonic anhydrases I, II and VI in the rat lingual serous salivary glands of von Ebner.","authors":"Robert S Redman","doi":"10.4081/ejh.2025.4159","DOIUrl":"10.4081/ejh.2025.4159","url":null,"abstract":"Carbonic anhydrase (CA) has been localized to many structures involved in ion transport including the acini and ducts of the major (parotid, sublingual and submandibular) salivary glands of humans and rodents. It also has been localized by enzyme histochemistry and by immunohistochemistry for CA isoenzyme VI (CA VI) to the acini and ducts of rat serous lingual glands of von Ebner. The purpose of this study was to explore the intracellular distribution by cell type of three CA isoenzymes in these glands. Immunohistochemistry was undertaken with antibodies to human CAs I, II and VI in paraffin sections of rat tongues that had been fixed in Helly's fluid. The density of the reaction product was scored as 0 (none) to 5 (strongest). Reactions in the acini with CA I and II antibodies were weak luminally to moderate basally and generally moderate, respectively, moderate in the intercalated ducts, and moderate basally to strong luminally in the excretory ducts. Weak to moderate CA VI reactions occurred in the acini and ducts. The stronger luminal reactions to CAs I and II in the excretory ducts suggest that they contribute to pH regulation in the saliva of von Ebner's glands via HCO3- transport.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 1","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-01-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11920962/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143544087","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0