European Journal of Histochemistry最新文献_第3页

Spinosin ameliorates osteoarthritis through enhancing the Nrf2/HO-1 signaling pathway. 刺五加皂苷能通过增强 Nrf2/HO-1 信号通路改善骨关节炎。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2024-05-22 DOI: 10.4081/ejh.2024.4033

Peipei Lu, Shuxiang Li, Caoyang Zhang, Xinyi Jiang, Jinghua Xiang, Hong Xu, Jian Dong, Kun Wang, Yuhua Shi

{"title":"Spinosin ameliorates osteoarthritis through enhancing the Nrf2/HO-1 signaling pathway.","authors":"Peipei Lu, Shuxiang Li, Caoyang Zhang, Xinyi Jiang, Jinghua Xiang, Hong Xu, Jian Dong, Kun Wang, Yuhua Shi","doi":"10.4081/ejh.2024.4033","DOIUrl":"10.4081/ejh.2024.4033","url":null,"abstract":"Osteoarthritis (OA) is a common degenerative joint disease in the elderly, while oxidative stress-induced chondrocyte degeneration plays a key role in the pathologic progression of OA. One possible reason is that the expression of nuclear factor erythroid 2-related factor 2 (Nrf2), which acts as the intracellular defense factor against oxidative stress, is significantly inhibited in chondrocytes. Spinosin (SPI) is a potent Nrf2 agonist, but its effect on OA is still unknown. In this study, we found that SPI can alleviate tert-Butyl hydroperoxide (TBHP)-induced extracellular matrix degradation of chondrocytes. Additionally, SPI can effectively activate Nrf2, heme oxygenase-1 (HO-1), and NADPH quinone oxidoreductase 1 (NQO1) in chondrocytes under the TBHP environment. When Nrf2 was silenced by siRNA, the cartilage protective effect of SPI was also weakened. Finally, SPI showed good alleviative effects on OA in mice. Thus, SPI can ameliorate oxidative stress-induced chondrocyte dysfunction and exhibit a chondroprotective effect through activating the Nrf2/HO-1 pathway, which may provide a novel and promising option for the treatment of OA.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11148693/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"141082849","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Emodin improves renal fibrosis in chronic kidney disease by regulating mitochondrial homeostasis through the mediation of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1α). 大黄素通过调节过氧化物酶体增殖体激活受体-γ辅助激活剂-1α（PGC-1α）来调节线粒体稳态，从而改善慢性肾病患者的肾脏纤维化。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2024-05-13 DOI: 10.4081/ejh.2024.3917

Liuchang Feng, Zaoqiang Lin, Zeyong Tang, Lin Zhu, Shu Xu, Xi Tan, Xinyuan Wang, Jianling Mai, Qinxiang Tan

{"title":"Emodin improves renal fibrosis in chronic kidney disease by regulating mitochondrial homeostasis through the mediation of peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1α).","authors":"Liuchang Feng, Zaoqiang Lin, Zeyong Tang, Lin Zhu, Shu Xu, Xi Tan, Xinyuan Wang, Jianling Mai, Qinxiang Tan","doi":"10.4081/ejh.2024.3917","DOIUrl":"10.4081/ejh.2024.3917","url":null,"abstract":"Chronic kidney disease (CKD) is a leading public health issue associated with high morbidity worldwide. However, there are only a few effective therapeutic strategies for CKD. Emodin, an anthraquinone compound from rhubarb, can inhibit fibrosis in tissues and cells. Our study aims to investigate the antifibrotic effect of emodin and the underlying molecular mechanism. A unilateral ureteral obstruction (UUO)-induced rat model was established to evaluate the effect of emodin on renal fibrosis development. Hematoxylin and eosin staining, Masson's trichrome staining, and immunohistochemistry staining were performed to analyze histopathological changes and fibrotic features after emodin treatment. Subsequently, a transforming growth factor-beta 1 (TGF-β1)-induced cell model was used to assess the inhibition of emodin on cell fibrosis in vitro. Furthermore, Western blot analysis and real-time quantitative reverse transcription-polymerase chain reaction were performed to validate the regulatory mechanism of emodin on renal fibrosis progression. As a result, emodin significantly improved histopathological abnormalities in rats with UUO. The expression of fibrosis biomarkers and mitochondrial biogenesis-related proteins also decreased after emodin treatment. Moreover, emodin blocked TGF-β1-induced fibrotic phenotype, lipid accumulation, and mitochondrial homeostasis in NRK-52E cells. Conversely, peroxisome proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1α) silencing significantly reversed these features in emodin-treated cells. Collectively, emodin plays an important role in regulating PGC-1α-mediated mitochondria function and energy homeostasis. This indicates that emodin exhibits great inhibition against renal fibrosis and acts as a promising inhibitor of CKD.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.1,"publicationDate":"2024-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11128849/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Erratum - LncRNA gadd7 promotes mitochondrial membrane potential decrease and apoptosis of alveolar type II epithelial cells by positively regulating MFN1 in an in vitro model of hyperoxia-induced acute lung injury. Erratum - 在高氧诱导的急性肺损伤体外模型中，LncRNA gadd7通过正向调节MFN1促进线粒体膜电位下降和肺泡II型上皮细胞凋亡。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2024-05-07 DOI: 10.4081/ejh.2024.4058

Guoyue Liu, Cunzhi Yin, Mingjiang Qian, Xuan Xiao, Hang Wu, Fujian Fu

引用次数: 0

Pleural mesothelioma from fluoro-edenite exposure: PACAP and PAC1 receptor. A preliminary report. 接触氟烯酸盐导致的胸膜间皮瘤：PACAP 和 PAC1 受体。初步报告。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-05-02 DOI: 10.4081/ejh.2024.3994

Claudia Lombardo, Grazia Maugeri, Agata Grazia D'Amico, Giuseppe Broggi, Rosario Caltabiano, Veronica Filetti, Serena Matera, Velia D'Agata, Carla Loreto

{"title":"Pleural mesothelioma from fluoro-edenite exposure: PACAP and PAC1 receptor. A preliminary report.","authors":"Claudia Lombardo, Grazia Maugeri, Agata Grazia D'Amico, Giuseppe Broggi, Rosario Caltabiano, Veronica Filetti, Serena Matera, Velia D'Agata, Carla Loreto","doi":"10.4081/ejh.2024.3994","DOIUrl":"10.4081/ejh.2024.3994","url":null,"abstract":"Pleural mesothelioma is a devastating malignancy primarily associated with asbestos exposure. However, emerging evidence suggests that exposure to fluoro-edenite fibers, a naturally occurring mineral fiber, can also lead to the development of pleural mesothelioma. In this study, based on the hypothesis that pituitary adenylate cyclase-activating polypeptide (PACAP) and PACAP-preferring receptor (PAC1R) expressions could be dysregulated in pleural mesothelioma samples and that they could potentially act as diagnostic or prognostic biomarkers, we aimed to investigate the immunohistochemical expression of PACAP and PAC1R in pleural biopsies from patients with pleural mesothelioma exposed to fluoro-edenite fibers. A total of 12 patients were included in this study, and their biopsies were processed for immunohistochemical analysis to evaluate the expression of PACAP and its receptor. The study revealed a correlation between the overexpression of PACAP and PAC1R and shorter overall survival in patients with malignant mesothelioma. These findings suggest that PACAP and PAC1R expression levels could serve as potential prognostic biomarkers for malignant mesothelioma. Furthermore, the immunohistochemical analysis of PACAP and PAC1R may provide valuable information for clinicians to guide therapeutic decisions and identify patients with poorer prognosis.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-05-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110723/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140867138","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction. 在脓毒症诱发的心肌功能障碍中，白细胞介素-15预处理可通过抑制NF-κB信号转导减轻炎症和细胞凋亡。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-04-29 DOI: 10.4081/ejh.2024.4019

Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang

{"title":"Pretreatment with interleukin-15 attenuates inflammation and apoptosis by inhibiting NF-κB signaling in sepsis-induced myocardial dysfunction.","authors":"Chaojie He, Yi Yu, Feifan Wang, Wudi Li, Hui Ni, Meixiang Xiang","doi":"10.4081/ejh.2024.4019","DOIUrl":"10.4081/ejh.2024.4019","url":null,"abstract":"Sepsis-induced myocardial dysfunction (SIMD) is associated with poor prognosis and increased mortality in patients with sepsis. Cytokines are important regulators of both the initiation and progression of sepsis. Interleukin-15 (IL-15), a pro-inflammatory cytokine, has been linked to protective effects against myocardial infarction and myocarditis. However, the role of IL-15 in SIMD remains unclear. We established a mouse model of SIMD via cecal ligation puncture (CLP) surgery and a cell model of myocardial injury via lipopolysaccharide (LPS) stimulation. IL-15 expression was prominently upregulated in septic hearts as well as cardiomyocytes challenged with LPS. IL-15 pretreatment attenuated cardiac inflammation and cell apoptosis and improved cardiac function in the CLP model. Similar cardioprotective effects of IL-15 pretreatment were observed in vitro. As expected, IL-15 knockdown had the opposite effect on LPS-stimulated cardiomyocytes. Mechanistically, we found that IL-15 pretreatment reduced the expression of the pro-apoptotic proteins cleaved caspase-3 and Bax and upregulated the anti-apoptotic protein Bcl-2. RNA sequencing and Western blotting further confirmed that IL-15 pretreatment suppressed the activation of nuclear factor kappa B (NF-κB) signaling in mice with sepsis. Besides, the addition of NF-κB inhibitor can significantly attenuate cardiomyocyte apoptosis compared to the control findings. Our results suggest that IL-15 pretreatment attenuated the cardiac inflammatory responses and reduced cardiomyocyte apoptosis by partially inhibiting NF-κB signaling in vivo and in vitro, thereby improving cardiac function in mice with sepsis. These findings highlight a promising therapeutic strategy for SIMD.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 2","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11110722/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873432","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Style head in Apocynaceae: a very complex secretory activity performed by one tissue. 天南星科植物的花柱头：由一个组织进行非常复杂的分泌活动。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-04-02 DOI: 10.4081/ejh.2024.4027

Danielle Maximo, Diego Demarco

{"title":"Style head in Apocynaceae: a very complex secretory activity performed by one tissue.","authors":"Danielle Maximo, Diego Demarco","doi":"10.4081/ejh.2024.4027","DOIUrl":"https://doi.org/10.4081/ejh.2024.4027","url":null,"abstract":"Nuptial glands are very diverse and associated with different pollination mechanisms. The greater the specificity in the pollen transfer mechanism from anther to stigma, the greater the morphological elaboration of flowers and functional complexity of the nuptial glands. In Apocynaceae, pollination mechanisms reached an extreme specificity, a fact that was only possible due to an extreme morphological synorganization and a profusion of floral glands. Although these glands are of different types, the vast majority have secretory cells only in the epidermis. In general, these epidermal cells produce many different compounds at the same time, and previous studies have demonstrated that in the style head, the functional complexity of epidermis has become even greater. Four types of style head are found in the family, which have different degrees of functional complexity in relation to the secretion produced and pollen dispersal mechanism. The secretion is fluid in types I, II and III, and the pollen is dispersed and adhered to the pollinator by the secretion produced by the style head. In type IV, the secretion hardens and acquires a specific shape, moulded by the spatial constraints of the adjacent floral organs. This evolutionary alteration is accompanied by changes in the structure and arrangement of the secretory cells, as well as in pollen aggregation and position of stigma. Histochemical analysis has shown that the secretion is mixed and highly complex, especially in the style head type IV, where the secretion, called translator, is formed by a rigid central portion, which adheres to the pollinator, and two caudicles that attach to two pollinia. The translator has a distinct composition in its different parts. Further studies are needed to answer the new questions that have arisen from the discovery of this highly functional complexity of the secretory tissue.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-04-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11017725/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140873431","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Feulgen reaction: from pink-magenta to rainbow fluorescent at the Maffo Vialli's School of Histochemistry. 费尔根反应：马福-维亚利组织化学学院从粉红-洋红到彩虹荧光。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-02-22 DOI: 10.4081/ejh.2024.3971

Giuliano Mazzini

{"title":"The Feulgen reaction: from pink-magenta to rainbow fluorescent at the Maffo Vialli's School of Histochemistry.","authors":"Giuliano Mazzini","doi":"10.4081/ejh.2024.3971","DOIUrl":"10.4081/ejh.2024.3971","url":null,"abstract":"For over a century, Palazzo Botta (Palace Botta) has housed the University of Pavia's Biomedical Institutes. Illustrious scientists have conducted research and taught at this Palace, making significant contributions to the advancement of natural, biological, and medical science. Among them, Camillo Golgi received the Nobel Prize for discovering the so-called \"black reaction.\" Following Golgi, the Palace continued to be a hub for the development of methodologies and reactions aimed at detecting and quantifying biological components. Maffo Vialli (in the Golgi stream) was the first to establish a Histochemistry Research Group, which began in the naturalistic field and later expanded to the biomedical area. Among the many histochemical studies initiated in the Palace, the Feulgen reaction undoubtedly played a significant role. This reaction, developed R. Feulgen and H. Rossenbeck in 1924, had significant international implications: numerous researchers then contributed to define its fine chemical details, which remained the subject of study for years, resulting in a massive international scientific literature. The Pavia School of Histochemistry also contributed to the evolution and application of this method, which has become a true benchmark in quantitative histochemistry. Giovanni Prenna and the CNR Centre for Histochemistry made significant contributions, as they were already focused on fluorescence cytochemistry. The Pavia researchers made significant contributions to the development of methodology and, in particular, instrumentation; the evolution of the latter resulted in the emergence of flow cytometry and an ever-increasing family of fluorescent probes, which somewhat overshadowed the Feulgen reaction for DNA quantification. The advent of monoclonal antibodies then contributed to the final explosion of flow cytometry in clinical application, almost making young neophytes forget that its roots date back to Feulgen.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-02-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059467/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139933979","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lectins as versatile tools to explore cellular glycosylation. 连接蛋白是探索细胞糖基化的多功能工具。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3959

Susan Brooks

{"title":"Lectins as versatile tools to explore cellular glycosylation.","authors":"Susan Brooks","doi":"10.4081/ejh.2024.3959","DOIUrl":"10.4081/ejh.2024.3959","url":null,"abstract":"Lectins are naturally occurring carbohydrate-binding proteins that are ubiquitous in nature and highly selective for their, often incompletely characterised, binding partners. From their discovery in the late 1880s to the present day, they have provided a broad palette of versatile tools for exploring the glycosylation of cells and tissues and for uncovering the myriad functions of glycosylation in biological systems. The technique of lectin histochemistry, used to map the glycosylation of tissues, has been instrumental in revealing the changing profile of cellular glycosylation in development, health and disease. It has been especially enlightening in revealing fundamental alterations in cellular glycosylation that accompany cancer development and metastasis, and has facilitated the identification of glycosylated biomarkers that can predict prognosis and may have utility in development of early detection and screening, Moreover, it has led to insights into the functional role of glycosylation in healthy tissues and in the processes underlying disease. Recent advances in biotechnology mean that our understanding of the precise binding partners of lectins is improving and an ever-wider range of lectins are available, including recombinant human lectins and lectins with enhanced, engineered properties. Moreover, use of traditional histochemistry to support a broad range of cutting-edge technologies and the development of high throughout microarray platforms opens the way for ever more sophisticated mapping - and understanding - of the glycome.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059468/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571940","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

1954-2024: 70 years of histochemical research with the European Journal of Histochemistry. 1954-2024：《欧洲组织化学杂志》组织化学研究 70 年。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3970

Carlo Pellicciari, Marco Biggiogera, Manuela Malatesta

引用次数: 0

Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles. 为幽灵赋予躯体：将分子定位在其发挥生物作用的地方。

IF 2 4区生物学

European Journal of Histochemistry Pub Date : 2024-01-29 DOI: 10.4081/ejh.2024.3950

Ernesto Capanna, CarloAlberto Redi

{"title":"Giving bodies to ghosts: locating molecules in the very place where they exert their biological roles.","authors":"Ernesto Capanna, CarloAlberto Redi","doi":"10.4081/ejh.2024.3950","DOIUrl":"10.4081/ejh.2024.3950","url":null,"abstract":"This paper reviews some of the goals of our investigations published over the years on Rivista di Istochimica Normale e Patologica, Basic and Applied Histochemistry, and the European Journal of Histochemistry - EJH. In a series of papers, we published some of the basic cytochemical features of the sperm cytodifferentiation process for the first time. This was a conceptual and practical prerequisite to the in situ quantitative evaluation of sperm DNA content. We showed that the discrepancy between the expected 1:2 ratio when comparing sperm versus somatic cell DNA content (sperm DNA content is always far low from the theoretical value) is due to DNA losses caused by the hydrochloric treatment entailed by the Feulgen reaction. The knowledge of the specific losses that occur during the various steps of the Feulgen reaction has allowed us to use it critically in Genome Size studies to highlight: - sperm aneuploidy in chromosomally derived subfertility; - the broad variability range of Mammalian genome sizes; - that termites are roaches (after decades of discussion on this topic). In addition, in a seminal paper on human oocytes, we showed (by transmission electron microscopy) a specific chromatin and cytoplasmic organization (both essential for further embryo development) linked to oocyte maturation arrest, a datum quite relevant to treating unmet therapeutic needs in human and veterinary reproduction.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"68 1","pages":""},"PeriodicalIF":2.0,"publicationDate":"2024-01-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11059455/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139571937","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0