European Journal of Histochemistry最新文献

Erratum - Localization of ανβ6 integrin-TGF-β1/Smad3, mTOR and PPARγ in experimental colorectal fibrosis. α -β 6整合素- tgf -β1/Smad3、mTOR和PPARγ在实验性结直肠纤维化中的定位。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 Epub Date: 2025-10-13 DOI: 10.4081/ejh.2025.4428

Giovanni Latella, Antonella Vetuschi, Roberta Sferra, Silvia Speca, Eugenio Gaudio

引用次数: 0

Expression of nestin, parvalbumin and otoferlin during cochlear development in the mouse: an immunofluorescence study. 小鼠耳蜗发育过程中巢蛋白、小白蛋白和耳蜗蛋白的表达：免疫荧光研究。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 DOI: 10.4081/ejh.2025.4242

Wenjing Liu, Yongchun Zhang, Cheng Liang, Shujuan Yang

{"title":"Expression of nestin, parvalbumin and otoferlin during cochlear development in the mouse: an immunofluorescence study.","authors":"Wenjing Liu, Yongchun Zhang, Cheng Liang, Shujuan Yang","doi":"10.4081/ejh.2025.4242","DOIUrl":"10.4081/ejh.2025.4242","url":null,"abstract":"To elucidate the proteins associated with cochlear development and auditory formation from a histomorphological point of view, this study examined the spatio-temporal expression pattern of nestin, parvalbumin, and otoferlin in the mouse cochlea from embryonic day 17 (E17) to postnatal day 28 (P28) using immunofluorescence. Our findings revealed that nestin was broadly expressed in developing otic mesenchyme cells beneath the basilar membrane, medial to the greater epithelial ridge, and adjacent to the developing stria vascularis during late embryonic stages (E17 and E18.5). From P1 to the onset of hearing (P14), nestin was primarily expressed in fibrocytes derived from otic mesenchyme cells in the spiral ligament and spiral limbus, as well as in tympanic border cells. Dual immunofluorescence staining of nestin with Isolectin B4 (IB4), a specific vascular endothelial marker, showed the location of nestin in the blood vessels within the cochlear lateral wall. Notably, in adults (P28), nestin expression was downregulated in the fibrocytes of the spiral ligament and spiral limbus but persisted in the tympanic border cells. Parvalbumin immunolabeling was consistently observed in spiral ganglion neurons (SGNs) and inner hair cells (IHCs) from E17 through adulthood. By P1, parvalbumin expression extended to all three rows of outer hair cells (OHCs) and persisted into adulthood. Transient parvalbumin expression was also noted in afferent nerve fibers innervating the IHCs during early postnatal stages. Otoferlin labeling was predominantly detected in the cytoplasm of IHCs, with limited temporal expression in OHCs from P6 to P10. Taken together, these results illustrated the dynamic expression of nestin, parvalbumin and otoferlin during cochlear development and suggested their important function in cochlear development.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505281/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126445","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Guilu Erxian oral liquid mitigates oxidative damage in spermatogonial cells via miR-6739-5p modulation and PI3K/AKT pathway activation: a functional histocytochemical study. 桂露二仙口服液通过miR-6739-5p调控和PI3K/AKT通路激活减轻精原细胞氧化损伤：一项功能性组织细胞化学研究。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 Epub Date: 2025-10-02 DOI: 10.4081/ejh.2025.4253

Zefeng Sun, Xinrong Fan, Zhenquan Liu

{"title":"Guilu Erxian oral liquid mitigates oxidative damage in spermatogonial cells via miR-6739-5p modulation and PI3K/AKT pathway activation: a functional histocytochemical study.","authors":"Zefeng Sun, Xinrong Fan, Zhenquan Liu","doi":"10.4081/ejh.2025.4253","DOIUrl":"https://doi.org/10.4081/ejh.2025.4253","url":null,"abstract":"Oxidative stress is a major contributor to male infertility, particularly oligoasthenozoospermia. This study aimed to investigate the cytoprotective mechanism of Guilu Erxian Oral Liquid (GLEX) against H₂O₂-induced oxidative damage in spermatogonial cells, focusing on miR-6739-5p regulation and activation of the PI3K/AKT pathway using histocytochemical approaches. An oxidative stress model was established in rat spermatogonial stem cells (SSCs) with 250 µM H₂O₂. Cell proliferation, apoptosis, reactive oxygen species (ROS) accumulation, and DNA oxidative damage were assessed using EdU incorporation, flow cytometry, immunofluorescence, and 8-hydroxy-2'-deoxyguanosine (8-OHdG) ELISA. Expression of miR-6739-5p and Phosphatidylinositol 3-Kinase/Protein Kinase B (PI3K/AKT) pathway components (PIK3CA, p-PI3K, p-AKT) was evaluated by RT-qPCR and Western blotting. The interaction between miR-6739-5p and PIK3CA was confirmed via dual-luciferase reporter assay. The cytoprotective effects of GLEX were examined through pre-treatment and quantified using histochemical and cytological markers. H₂O₂ treatment significantly impaired cell viability, increased apoptosis and ROS production, and upregulated miR-6739-5p. Overexpression of miR-6739-5p exacerbated damage, while silencing reversed it and restored PI3K/AKT signaling. GLEX pretreatment effectively reduced miR-6739-5p expression, restored cell viability, suppressed oxidative and inflammatory markers (ROS, 8-OHdG, TNF-α, IL-1β), and enhanced PI3K/AKT activation. These effects were comparable to PI3K pathway activation. GLEX confers histocytochemical protection to spermatogonial cells under oxidative stress by downregulating miR-6739-5p and activating the PI3K/AKT pathway. This study highlights a novel regulatory mechanism and supports GLEX as a potential therapeutic agent for oxidative stress-associated male infertility.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145208416","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-129-5p regulates HMGB1/RAGE axis to inhibit pyroptosis and ameliorate cervical epithelial cell deterioration. miR-129-5p调节HMGB1/RAGE轴抑制焦亡，改善宫颈上皮细胞退化。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 Epub Date: 2025-09-23 DOI: 10.4081/ejh.2025.4238

Huafang Wang, Dilidaer Sidike, Pan Liu, Longge Suo, Huerxidan Niyazi

{"title":"miR-129-5p regulates HMGB1/RAGE axis to inhibit pyroptosis and ameliorate cervical epithelial cell deterioration.","authors":"Huafang Wang, Dilidaer Sidike, Pan Liu, Longge Suo, Huerxidan Niyazi","doi":"10.4081/ejh.2025.4238","DOIUrl":"10.4081/ejh.2025.4238","url":null,"abstract":"Cervical cancer is a serious gynecological malignancy, and the specific mechanisms of miR-129-5p remain unclear. This study aims to investigate the mechanism by which miR-129-5p regulates the high mobility group box 1 (HMGB1/receptor for advanced glycation end-products (RAGE) axis to inhibit pyroptosis and ameliorate cervical epithelial cell deterioration. Using RT-qPCR and Western blotting, we detected significantly downregulated miR-129-5p and upregulated HMGB1 in cervical cancer cells. To establish a deterioration model, we stimulated cervical epithelial cells with lipopolysaccharide (LPS). Further results revealed that miR-129-5p overexpression markedly reduced HMGB1 expression, suppressed RAGE activation, and decreased pyroptosis executer GSDMD-N production. Additionally, we conducted miR-129-5p overexpression and knockdown experiments to verify its regulatory effects on the HMGB1/RAGE axis and downstream pathways. Caspase-1 activity assays confirmed reduced pyroptosis upon miR-129-5p overexpression. Cell viability and proliferation were assessed using EdU incorporation assays and colony formation experiments. Our data demonstrated significant downregulation of miR-129-5p in cervical cancer cells. Overexpression of miR-129-5p substantially reduced HMGB1 expression and inhibited RAGE activation, thereby decreasing production of the pyroptosis executer GSDMD-N. LPS stimulation potently activated the HMGB1/RAGE axis and induced pyroptosis, while miR-129-5p overexpression inhibited these processes and ameliorated in vitro cervical epithelial cell deterioration. Cells overexpressing miR-129-5p exhibited attenuated caspase-1 activity with enhanced survival and proliferation following LPS treatment. Collectively, these in vitro findings indicate that miR-129-5p suppresses HMGB1/RAGE-mediated pyroptosis and cellular deterioration and also provide new mechanistic insights for cervical cancer therapeutics.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505279/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126381","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-199a-5p inhibited HIF-1α to suppress the proliferation, migration, and differentiation of cardiac stem cells. miR-199a-5p通过抑制HIF-1α抑制心脏干细胞的增殖、迁移和分化。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 DOI: 10.4081/ejh.2025.4239

Sha Li, Cuigai Zhang, Yanping Liu, Cuiqiao Meng, Yang Xie, Shuren Li

{"title":"miR-199a-5p inhibited HIF-1α to suppress the proliferation, migration, and differentiation of cardiac stem cells.","authors":"Sha Li, Cuigai Zhang, Yanping Liu, Cuiqiao Meng, Yang Xie, Shuren Li","doi":"10.4081/ejh.2025.4239","DOIUrl":"10.4081/ejh.2025.4239","url":null,"abstract":"The cardiac stem cells (CSCs) are essential in improving myocardial infarction (MI). Although miR-199a-5p and hypoxia-inducible factor-1 alpha (HIF-1α) were proven to participate in the process of heart repair, the related mechanisms are still unclear. This study aimed to explore the effects of miR-199a-5p and HIF-1α on c-kit+ cells and their regulatory mechanisms. After isolating, purifying, and identifying CSCs (c-kit+ cells) from mice, they were subjected to a hypoxia model. After the c-kit+ cells were transfected with corresponding transfectants, the CCK-8, EdU staining, and wound healing approaches were used to evaluate their cell viability, proliferation, and migration. The targeted relation between miR-199a-5p and HIF-1α was determined using a dual-luciferase reporter. Immunofluorescence staining, RT-qPCR, and Western blot approaches were employed to determine Nkx2.5, CD31, α-SMA, miR-199a-5p, and HIF-1α expression. Overexpressing miR-199a-5p and knocking down HIF-1α both inhibited the cell viability (p<0.01), reduced the proliferation (p<0.05), suppressed the migration (p<0.001), and down-regulated the Nkx2.5, CD31, and α-SMA expression of c-kit+ cells (p<0.05). Overexpressing HIF-1α effectively reversed the effects of overexpressing miR-199a-5p on c-kit+ cells (p<0.05). Taken together, miR-199a-5p negatively targeted HIF-1α to inhibit the proliferation, migration, and differentiation of c-kit+ cells.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12505280/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145126456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Maresin 1 alleviates myocardial ischemia-reperfusion injury in rats by suppressing inflammation. 马瑞辛1通过抑制炎症反应减轻大鼠心肌缺血再灌注损伤。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-09-22 Epub Date: 2025-10-13 DOI: 10.4081/ejh.2025.4254

Hongkai Xiao, Jinghu Liu, Qinhong Cai, Siyu Liang, Zhitao Hu, Xiaochao Chen

{"title":"Maresin 1 alleviates myocardial ischemia-reperfusion injury in rats by suppressing inflammation.","authors":"Hongkai Xiao, Jinghu Liu, Qinhong Cai, Siyu Liang, Zhitao Hu, Xiaochao Chen","doi":"10.4081/ejh.2025.4254","DOIUrl":"https://doi.org/10.4081/ejh.2025.4254","url":null,"abstract":"Myocardial ischemia-reperfusion injury (MIRI) induces severe inflammatory damage to cardiac tissue, leading to structural impairment and functional decline. Maresin 1 (MaR1) is an anti-inflammatory lipid mediator derived from macrophages that has shown protective effects in various inflammatory conditions. This study investigated the anti-inflammatory properties and underlying mechanisms of MaR1 in the context of MIRI, both in vivo and in vitro. A rat model of MIRI was established, and MaR1 was administered subcutaneously once daily for one week prior to model induction. Cardiac function was monitored intraoperatively, and serum and myocardial tissue samples were collected postoperatively for analysis. Structural alterations, myocardial injury biomarkers, and inflammatory cytokines were evaluated. In vitro experiments using H9c2 rat cardiomyocytes assessed the effects of MaR1 on cell viability and proliferation. MaR1 treatment significantly improved cardiac function impaired by MIRI, preserved myocardial architecture, and reduced serum and tissue levels of creatine kinase, lactate dehydrogenase, cardiac troponin I, and pro-inflammatory cytokines (IL-1β, IL-6, IL-8, MCP1, and TNF-α). In contrast, MaR1 enhanced the expression of the anti-inflammatory cytokine IL-10. In cultured cardiomyocytes, MaR1 promoted viability and proliferation. Collectively, these findings demonstrate that MaR1 confers protection against MIRI by attenuating inflammation, preserving myocardial structure, improving cardiac function, and enhancing cardiomyocyte survival, underscoring its potential as a therapeutic agent for ischemic cardiac injury.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 4","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-09-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145281548","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tanshinone IIA attenuates hepatic stellate cell activation, oxidative stress, and liver fibrosis by inhibiting YAP signaling. 丹参酮IIA通过抑制YAP信号通路减弱肝星状细胞活化、氧化应激和肝纤维化。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-06-17 DOI: 10.4081/ejh.2025.4218

Dan Wang, Qingquan Tan, Qing Zheng, Yanling Ma, Li Feng

{"title":"Tanshinone IIA attenuates hepatic stellate cell activation, oxidative stress, and liver fibrosis by inhibiting YAP signaling.","authors":"Dan Wang, Qingquan Tan, Qing Zheng, Yanling Ma, Li Feng","doi":"10.4081/ejh.2025.4218","DOIUrl":"10.4081/ejh.2025.4218","url":null,"abstract":"Tanshinone IIA is derived from Salvia miltiorrhiza and has multiple therapeutic targets and functions. The exact therapeutic effects on liver fibrosis as well as the underlying hepatoprotective mechanisms are still lacking. A liver fibrosis model was established via ligation of the common bile duct ligation (BDL). The mice were intraperitoneally administered different concentrations of tanshinone IIA (4 mg/kg, 8 mg/kg) for 2 weeks. Liver function was assessed through hematoxylin and eosin and Sirus red staining. Serum levels of aspartate aminotransferase (AST), alanine aminotransferase (ALT), glutathione (GSH) and malondialdehyde (MDA) were quantified by enzyme-linked immunosorbent assay (ELISA), via microplate reader. The total iron content of the liver was quantified via Triple Quad-ICP-MS. TGFβ-induced hepatic stellate cells (HSCs), a cell model of liver fibrosis, were treated with tanshinone IIA at different concentrations (10 mM, 20 mM, 30 mM, 40 mM). The combination of tanshinone IIA with YAP agonists was applied in activated HSCs and animal models. Tanshinone IIA treatment relieved BDL-induced liver fibrosis; mitigated histological liver damage; lowered the serum ALT and AST levels; reduced macrophage infiltration and the MDA and iron contents; and increased the GSH and GPX4 levels by inhibiting YAP signaling. tanshinone IIA also suppressed the activation of HSCs and collagen production through blocking the YAP signaling pathway. The YAP agonist reversed the therapeutic effect of tanshinone IIA on activated HSCs and BDL-induced liver fibrosis. Tanshinone IIA inhibited HSC activation and oxidative stress and alleviated liver fibrosis by inhibiting the YAP signaling pathway.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12210874/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144318568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histological and histochemical characterization of the musk gland in forest musk deer (Moschus berezovskii): a preliminary study. 森林麝麝香腺的组织学和组织化学特征：初步研究。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-06-17 Epub Date: 2025-07-10 DOI: 10.4081/ejh.2025.4216

Qianyi Wang, Chunyu Han, Dong Zhang, Yuning Liu, Yunyun Gao, Haolin Zhang, Defu Hu

{"title":"Histological and histochemical characterization of the musk gland in forest musk deer (Moschus berezovskii): a preliminary study.","authors":"Qianyi Wang, Chunyu Han, Dong Zhang, Yuning Liu, Yunyun Gao, Haolin Zhang, Defu Hu","doi":"10.4081/ejh.2025.4216","DOIUrl":"10.4081/ejh.2025.4216","url":null,"abstract":"Musk is a biologically valuable secretion from the musk gland of male musk deer, with significant economic and medicinal importance. Due to severe decline and depletion of wild musk deer population, captive breeding of musk deer has become the primary approach for sustainable musk production. So far, the histological structure and secretion mechanism of the musk gland remain incompletely understood. In this study, we employed histological and immunohistochemical (IHC) techniques, along with three-dimensional (3D) tissue reconstruction, to systematically analyze the cellular composition and secretory functions of the musk gland in forest musk deer (Moschus berezovskii). Our results revealed that the musk gland was primarily composed of acinar structures containing two distinct glandular cell (GC) types based on the histological observation. IHC results showed type I glandular cells (GCIs) predominantly expressed GALNT7 while type II glandular cells (GCIIs) mainly expressed BMP6. The 3D reconstruction demonstrated structural heterogeneity along the gland's longitudinal axis, with the proportion of the acinar area varying between 40% and 65%. This is the first time that a detailed 3D view of musk gland in forest musk deer has been shown, which provides essential histological insights into musk gland function in this species. These preliminary observations may provide useful groundwork for future investigations into the regulatory mechanisms of musk secretion.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12282573/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats. 成年大鼠空肠和结肠中骨形态发生蛋白信号通路参与者的表达。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-06-17 Epub Date: 2025-08-21 DOI: 10.4081/ejh.2025.4174

Emma Cogo, Edwin Fouché, Charline Buisson, Adenike Omotoyinbo, Fabrice Pierre, Françoise Guéraud, Pascale Plaisancié

{"title":"Expression of bone morphogenetic protein signaling pathway players in the jejunum and colon of adult rats.","authors":"Emma Cogo, Edwin Fouché, Charline Buisson, Adenike Omotoyinbo, Fabrice Pierre, Françoise Guéraud, Pascale Plaisancié","doi":"10.4081/ejh.2025.4174","DOIUrl":"10.4081/ejh.2025.4174","url":null,"abstract":"The bone morphogenetic protein (BMP) pathway, which plays a crucial role in the control of intestinal epithelial cell homeostasis, has been studied in mice and humans, leading to an understanding of its involvement in several intestinal pathologies. However, the expression and localization of the various actors (ligands, antagonists, receptors) of this pathway remain unknown in the rat intestine, although this species is widely used in pathophysiology studies. Here, we aimed to determine the expression and localization of the various players in the BMP pathway in the jejunum and colon of the rat using RT-qPCR and immunohistochemistry. BMP2, mainly localized in epithelial cells, was the most expressed ligand in the jejunum and colon in comparison with BMP4, BMP6 and BMP7. We showed for the first time that BMP7 was highly expressed in epithelial cells in both tissues. BMP2, BMP6 and BMP7 ligands were also present in the enteric nervous plexuses, as the BMP receptors and antagonists Noggin and Chordin-like 1. The expression of BMP antagonists and ligands in enterocytes and mature colonocytes could suggest a paracrine or autocrine feedback modulation at the cellular level. Finally, all the studied BMP actors were present in colonic vessel walls including GREM1, a BMP antagonist described as pro-angiogenic and also being a ligand for VEGFR receptors. These data provided a good correlation between the observations in rats compared to those in humans and highlighted the importance of the BMP pathway not only in the intestinal epithelium, but also in both the enteric nervous system and vascular system. Our work lays the foundations for further studies on the involvement of the BMP pathway in rat models of intestinal pathophysiology.","PeriodicalId":50487,"journal":{"name":"European Journal of Histochemistry","volume":"69 3","pages":""},"PeriodicalIF":2.1,"publicationDate":"2025-06-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC12421720/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144977261","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Targeting EphA2 suppresses the proliferation, migration and invasion of endometriosis via the AMPK signaling pathway. 靶向EphA2通过AMPK信号通路抑制子宫内膜异位症的增殖、迁移和侵袭。

IF 2.1 4区生物学

European Journal of Histochemistry Pub Date : 2025-06-17 DOI: 10.4081/ejh.2025.4168

Chaoyi Yang, Shujun Wang, Mengru Li, Xiangli Pang, Aili Tan

引用次数: 0