FEBS Letters最新文献

{"title":"Arabidopsis RAN GTPases are critical for mitosis during male and female gametogenesis","authors":"Zheng Qin, Yanan Wu, T. Sun, Ting Ma, Meng Xu, Chen Pang, Sha Li, Sha Li","doi":"10.1002/1873-3468.14422","DOIUrl":"https://doi.org/10.1002/1873-3468.14422","url":null,"abstract":"The development of male and female gametophytes is a prerequisite for successful propagation of angiosperms. The small GTPases RAN play fundamental roles in numerous cellular processes. Although RAN GTPases have been characterized in plants, their roles in cellular processes are far from understood. We report here that RAN GTPases in Arabidopsis are critical for gametophytic development. RAN1 loss‐of‐function showed no defects in gametophytic development likely due to redundancy. However, the expression of a dominant negative or constitutively active RAN1 resulted in gametophytic lethality. Genetic interference of RAN GTPases caused the arrest of pollen mitosis I and of mitosis of functional megaspores, implying a key role of properly regulated RAN activity in mitosis during gametophytic development.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-06-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"46336172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Universal Stress Protein regulates the circadian rhythm of central oscillator genes in Arabidopsis","authors":"K. A. Phan, Seol Ki Paeng, H. Chae, Joung Hun Park, Eun Seon Lee, Seong Dong Wi, Su Bin Bae, M. G. Kim, D. Yun, Woe-Yeon Kim, Sang Yeol Lee","doi":"10.1002/1873-3468.14410","DOIUrl":"https://doi.org/10.1002/1873-3468.14410","url":null,"abstract":"Environmental stresses restrict plant growth and development and decrease crop yield. The circadian clock is associated with the ability of a plant to adapt to daily environmental fluctuations and the production and consumption of energy. Here, we investigated the role of Arabidopsis Universal Stress Protein (USP; At3g53990) in the circadian regulation of nuclear clock genes. The Arabidopsis usp knockout mutant line exhibited critically diminished circadian amplitude of the central oscillator CIRCADIAN CLOCK ASSOCIATED 1 (CCA1) but enhanced the amplitude of TIMING OF CAB EXPRESSION 1 (TOC1). However, the expression of USP under the control of its own promoter restored the circadian timing of both genes, suggesting that USP regulates the circadian rhythm of Arabidopsis central clock genes, CCA1 and TOC1.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45032644","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hidden biochemical fossils reveal an evolutionary trajectory for glycolysis in the prebiotic era","authors":"M. Kalapos, L. de Bari","doi":"10.1002/1873-3468.14408","DOIUrl":"https://doi.org/10.1002/1873-3468.14408","url":null,"abstract":"Glycolysis is present in nearly all organisms alive today. This article proposes an evolutionary trajectory for the development of glycolysis in the framework of the chemoautotrophic theory for the origin of life. In the proposal, trioses and triose‐phosphates were appointed to starting points. The six‐carbon and the three‐carbon intermediates developed in the direction of gluconeogenesis and glycolysis, respectively, differing from the from‐bottom‐to‐up development of enzymatic glycolysis. The examination of enzymatic reaction mechanisms revealed that the enzymes incorporated chemical mechanisms of the nonenzymatic stage, making possible to identify kinship between glyoxalases and glyceraldehyde 3‐phosphate dehydrogenase as well as methylglyoxal synthase and triose‐phosphate isomerase. This developmental trajectory may shed light on how glycolysis might have developed in the nonenzymatic era.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44574193","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The potassium channel GhAKT2bD is regulated by CBL–CIPK calcium signalling complexes and facilitates K+ allocation in cotton","authors":"Rui Zhang, Qiuyan Dong, Panpan Zhao, Anna Eickelkamp, Chunmin Ma, Gefeng He, Fangjun Li, L. Wallrad, Tobias Becker, Zhaohu Li, J. Kudla, X. Tian","doi":"10.1002/1873-3468.14377","DOIUrl":"https://doi.org/10.1002/1873-3468.14377","url":null,"abstract":"Efficient allocation of the essential nutrient potassium (K+) is a central determinant of plant ion homeostasis and involves AKT2 K+ channels. Here, we characterize four AKT2 K+ channels from cotton and report that xylem and phloem expressed GhAKT2bD facilitates K+ allocation and that AKT2‐silencing impairs plant growth and development. We uncover kinase activity‐dependent activation of GhAKT2bD‐mediated K+ uptake by AtCBL4–GhCIPK1 calcium signalling complexes in HEK293T cells. Moreover, AtCBL4–AtCIPK6 complexes known to convey activation of AtAKT2 in Arabidopsis also activate cotton GhAKT2bD in HEK293T cells. Collectively, these findings reveal an essential role for AKT2 in the source‐sink allocation of K+ in cotton and identify GhAKT2bD as subject to complex regulation by CBL–CIPK Ca2+ sensor–kinase complexes.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45482989","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Cover","authors":"","doi":"10.1002/1873-3468.14119","DOIUrl":"https://doi.org/10.1002/1873-3468.14119","url":null,"abstract":"","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"42919999","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ergothioneine, where are we now?","authors":"B. Halliwell, I. Cheah","doi":"10.1002/1873-3468.14350","DOIUrl":"https://doi.org/10.1002/1873-3468.14350","url":null,"abstract":"The water-soluble thione/thiol ergothioneine (ET) was first isolated in 1909 by Charles Tanret [1], from the ergot fungus Claviceps purpurea. This fungus is notorious for the toxicity of some of its metabolites to humans, causing ergotism [2], which has even been linked to the Salem witch trials [3]. However, ergotism has nothing to do with ET, which is instead very safe for human consumption and is synthesized by a range of other fungi and some bacteria (reviewed in [4–9]). Its biosynthetic pathways are reviewed in detail in [6]. Indeed, as far as we know, humans and other animals obtain all their ET from the diet [4,5,7–10], whereas plants seem to obtain it from fungi and other soil microorganisms [9]. An enormous amount of work was done on ET in the 1950s, as summarized in the excellent review by Melville [7]. Interest then waned but has picked up rapidly in recent years (Fig. 1). We, therefore, thought that it was about time for a collection of articles and reviews highlighting the recent developments in the ET field. We, thus, approached both FEBS Letters, which was very supportive, and a range of experts working on ET, who were almost uniformly enthusiastic and happy to contribute. The FEBS Letters Special Issue ‘Ergothioneine, where are we now?’ is the result of these activities and contains 11 articles by leading experts. One catalyst for this upsurge of interest was the discovery in 2005 of a transporter for ET (OCTN1, often now called the ergothioneine transporter, ETT), which accounts for the fact that animals (including humans) take up and avidly retain ET from the diet [11]. The specificity of ETT for ET has often been challenged but has been reconfirmed in several studies [11–13], as reviewed in depth by Grundemann et al. in this special issue [14]. The presence of a specific transporter together with the avid retention of ET in the body implies that this compound is important to us, and indeed in 2018 Bruce Ames proposed that ET be classified as a ‘longevity vitamin’ [15]. No specific deficiency disease has yet been identified for ET, which makes it hard to formally classify it as a vitamin. Perhaps, however, deficiency diseases are staring us in the face: low blood or plasma levels of ET are correlated with increased risk of frailty [16–18], cardiovascular disease [19], mild cognitive impairment [18, 20–22], dementia [22,23] and Parkinson’s disease [24]. Indeed, ET has many neuroprotective properties [4,5,18,26,27], as reviewed in detail in this special issue [18,25,26]. Consistent with a key protective role of ET against the development of age-related diseases, higher dietary consumption of mushrooms, a rich source of ET [9], is associated with lower disease risk [28–31]. However, we must be cautious; to quote an old phrase ‘correlation does not imply causation’. Low ET levels may predispose to disease, but disease could also lead to low ET levels. Possible reasons could include alterations in diet due to illness so that less ET ","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"48004025","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"A native promoter–gene fusion created by CRISPR/Cas9‐mediated genomic deletion offers a transgene‐free method to drive oil accumulation in leaves","authors":"R. Bhunia, Guillaume N. Menard, P. Eastmond","doi":"10.1002/1873-3468.14365","DOIUrl":"https://doi.org/10.1002/1873-3468.14365","url":null,"abstract":"Achieving gain‐of‐function phenotypes without inserting foreign DNA is an important challenge for plant biotechnologists. Here, we show that a gene can be brought under the control of a promoter from an upstream gene by deleting the intervening genomic sequence using dual‐guide CRISPR/Cas9. We fuse the promoter of a nonessential photosynthesis‐related gene to DIACYLGLYCEROL ACYLTRANSFERASE 2 (DGAT2) in the lipase‐deficient sugar‐dependent 1 mutant of Arabidopsis thaliana to drive ectopic oil accumulation in leaves. DGAT2 expression is enhanced more than 20‐fold and the triacylglycerol content increases by around 30‐fold. This deletion strategy offers a transgene‐free route to engineering traits that rely on transcriptional gain‐of‐function, such as producing high lipid forage to increase the productivity and sustainability of ruminant farming.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-05-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"43968397","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Front Cover","authors":"","doi":"10.1002/1873-3468.14116","DOIUrl":"https://doi.org/10.1002/1873-3468.14116","url":null,"abstract":"","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41772023","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"GENIGMA: an app to map the 3D genome of cancer cell lines through extreme citizen science","authors":"D. Ruffell","doi":"10.1002/1873-3468.14331","DOIUrl":"https://doi.org/10.1002/1873-3468.14331","url":null,"abstract":"In this Scientists' Forum article, we present a new app, GENIGMA, launched by structural genomics specialist Marc A. Marti‐Renom and his team, aimed at mapping the 3D genome of cancer cell lines. GENIGMA is a digital game, which was designed and tested through a co‐creation process with citizens. Finally, by playing the game, citizens produce data, reaching beyond the capacity of artificial intelligence. This is an exceptional experiment of extreme citizen science, which broadens the implications of open science.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-03-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"44749506","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Promiscuous activity of β‐carotene hydroxylase CrtZ on epoxycarotenoids leads to the formation of rare carotenoids with 6‐hydroxy‐3‐keto‐ε‐ends","authors":"Maiko Furubayashi, T. Maoka, Y. Mitani","doi":"10.1002/1873-3468.14342","DOIUrl":"https://doi.org/10.1002/1873-3468.14342","url":null,"abstract":"Carotenoids with rare 6‐hydroxy‐3‐keto‐ε‐end groups, such as piprixanthin, vitixanthin, or cochloxanthin, found in manakin birds or plants, are rare carotenoids with high antioxidant activity. The same chemical structure is found in abscisic acid or blumenol, apocarotenoids found in plants or fungi. In this study, we serendipitously discovered that the promiscuous activity of the β‐carotene hydroxylase CrtZ, a diiron‐containing membrane protein, can catalyze the formation of 6‐hydroxy‐3‐keto‐ε‐end by using epoxycarotenoids antheraxanthin or violaxanthin as substrate. We suggest that the reaction mechanism is similar to that of a rhodoxanthin biosynthetic enzyme. Our results provide a further understanding of the reaction mechanism of diiron‐containing β‐carotene hydroxylases, as well as insight into the biosynthesis of natural compounds with 6‐hydroxy‐3‐keto‐ε‐end carotenoid derivatives.","PeriodicalId":50454,"journal":{"name":"FEBS Letters","volume":null,"pages":null},"PeriodicalIF":3.5,"publicationDate":"2022-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"45512275","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}