Arabidopsis RAN GTPases are critical for mitosis during male and female gametogenesis

IF 3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

FEBS Letters Pub Date : 2022-06-09 DOI:10.1002/1873-3468.14422

Zheng Qin, Yanan Wu, T. Sun, Ting Ma, Meng Xu, Chen Pang, Sha Li, Sha Li

引用次数: 0

Abstract

The development of male and female gametophytes is a prerequisite for successful propagation of angiosperms. The small GTPases RAN play fundamental roles in numerous cellular processes. Although RAN GTPases have been characterized in plants, their roles in cellular processes are far from understood. We report here that RAN GTPases in Arabidopsis are critical for gametophytic development. RAN1 loss‐of‐function showed no defects in gametophytic development likely due to redundancy. However, the expression of a dominant negative or constitutively active RAN1 resulted in gametophytic lethality. Genetic interference of RAN GTPases caused the arrest of pollen mitosis I and of mitosis of functional megaspores, implying a key role of properly regulated RAN activity in mitosis during gametophytic development.

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拟南芥RAN gtpase在雄性和雌性配子体发生过程中对有丝分裂至关重要

雌雄配子体的发育是被子植物成功繁殖的先决条件。小的gtp酶在许多细胞过程中起着重要作用。尽管RAN GTPases已经在植物中被表征，但它们在细胞过程中的作用还远未被理解。我们在这里报道了RAN GTPases在拟南芥配子体发育中是至关重要的。RAN1的功能丧失在配子体发育中没有显示出可能由于冗余而导致的缺陷。然而，显性阴性或构成活性RAN1的表达导致配子体致死。遗传干扰RAN gtpase导致花粉有丝分裂I和功能大孢子有丝分裂停止，表明在配子体发育过程中，RAN活性的适当调节在有丝分裂中起关键作用。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

FEBS Letters 生物-生化与分子生物学

CiteScore

6.60

自引率

2.90%

发文量

303

审稿时长

1 months

期刊介绍： FEBS Letters is one of the world''s leading journals in molecular biology and is renowned both for its quality of content and speed of production. Bringing together the most important developments in the molecular biosciences, FEBS Letters provides an international forum for Minireviews, Research Letters and Hypotheses that merit urgent publication.