Journal of Membrane Biology最新文献_第2页

Peptide-Assisted Membrane Permeabilization: Effects of Waveform Polarity, Inter-Monopulse Interval, and Surface Charge in Red Blood Cells and Planar Lipid Bilayers. 肽辅助膜渗透：波形极性、单脉冲间隔和红细胞及平面脂质双层表面电荷的影响。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2026-01-07 DOI: 10.1007/s00232-025-00366-z

José Alexander Alvarez-Bustamante, Diego Ortiz-Mejía, Victor V Lemeshko

{"title":"Peptide-Assisted Membrane Permeabilization: Effects of Waveform Polarity, Inter-Monopulse Interval, and Surface Charge in Red Blood Cells and Planar Lipid Bilayers.","authors":"José Alexander Alvarez-Bustamante, Diego Ortiz-Mejía, Victor V Lemeshko","doi":"10.1007/s00232-025-00366-z","DOIUrl":"https://doi.org/10.1007/s00232-025-00366-z","url":null,"abstract":"Permeabilization of biomembranes by polycationic peptides is known to depend on the membrane potential, although the exact mechanism of this process is not yet completely defined, to be effectively controlled. We quantified peptide-assisted permeabilization of red blood cells (RBCs) using a custom system that delivered microsecond bipulses configured as bimonopolar (BMP; same polarity) or bipolar (BP; opposite polarity), varying the inter-monopulse interval ([Formula: see text]). In RBC suspensions, bulk light transmittance at 650 nm showed that BMP yielded higher permeabilization effect than BP at short [Formula: see text], whereas lengthening [Formula: see text] mitigated bipolar cancellation and increased BP responses. Membrane surface charge modulation by moderate concentrations of deoxycholate and spermine increased and decreased, respectively, membrane permeabilization effects of polycationic peptides. Complementary measurements in planar lipid bilayers (BLMs) under an applied command voltage ([Formula: see text]) with polarity alternation showed remarkable conduction at negative bias, essentially decreased in the presence of 1 mM Mg2+. Infrared thermometry over RBC suspension revealed modest heating (≈ 2.5-6 °C), equal for BMP and BP applications, indicating an electrical rather than thermal origin for waveform effects. Finally, the designed electroporation protocols allow controlled short-time permeabilization of cell membrane that might be useful for biotechnological applications and therapeutic delivery.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":"259 1","pages":"2"},"PeriodicalIF":2.9,"publicationDate":"2026-01-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145913680","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrative Multiscale Simulations Uncover the Mechanistic Principles Governing COPII Mediated Vesicle Formation. 综合多尺度模拟揭示了COPII介导的囊泡形成的机制原理。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-23 DOI: 10.1007/s00232-025-00367-y

Sanjoy Paul

引用次数: 0

Hypoxia Modulates Membrane Mechanics in Pancreatic Cancer. 缺氧调节胰腺癌的膜力学。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-08-18 DOI: 10.1007/s00232-025-00358-z

Prema Kumari Agarwala, Shobhna Kapoor

{"title":"Hypoxia Modulates Membrane Mechanics in Pancreatic Cancer.","authors":"Prema Kumari Agarwala, Shobhna Kapoor","doi":"10.1007/s00232-025-00358-z","DOIUrl":"10.1007/s00232-025-00358-z","url":null,"abstract":"Aggressive cancer cells such as pancreatic cells exhibit an enhanced metastatic phenotype that involves cell migration and invasion. Cellular membrane deformation is a key process implicit in cell movement. This implicates a link between altered lipid metabolism during cancer progression and modulated membrane properties and hence associated functions. One of the key factors underlying the aggressiveness of pancreatic cancer is the presence of the highest percentage of hypoxia, which further adds to the lipid metabolic reprogramming. The subsequent effect of hypoxia-induced lipidome changes on membrane properties governing cell movement was investigated in this work using a combination of cell biology, microscopy, and spectroscopy. Our findings revealed that hypoxia induces distinct lipidome signatures in a cell-line-dependent fashion, which in turn differentially modulates the cell membrane stiffness. The correlation of cell stiffness with other membrane properties and the actin cytoskeleton shows a random correlation indicating that hypoxic stress distinctly regulates specific membrane attributes governing cellular functioning and should be consulted for the development of effective treatments for pancreatic cancer.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"461-472"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144876537","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular Insights into Interactions between Ofloxacin and Ionic Micelles. 氧氟沙星与离子胶束相互作用的分子研究。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-07-22 DOI: 10.1007/s00232-025-00357-0

Saqib Rabbani, Areesha Maryam, Muhammad Sohail, Athar Yaseen Khan

{"title":"Molecular Insights into Interactions between Ofloxacin and Ionic Micelles.","authors":"Saqib Rabbani, Areesha Maryam, Muhammad Sohail, Athar Yaseen Khan","doi":"10.1007/s00232-025-00357-0","DOIUrl":"10.1007/s00232-025-00357-0","url":null,"abstract":"The growing antimicrobial resistance presents a challenge in developing new potent drugs, but this effort is hindered by a lack of information regarding how these new drugs would behave in biomembranes. Surfactants are considered mimetic models for biomembranes and can be used to study drug-membrane interactions. In this study, we used two well-known surfactants-cationic cetyltrimethylammonium bromide and anionic sodium dodecyl sulfate-as model membranes to investigate their interaction with the antimicrobial drug ofloxacin (OFL). These interactions were studied using volumetric and acoustic methods over the temperature range of 293.15-323.15 K to determine the apparent molar volume, isentropic compressibility, apparent molar compressibility, acoustic impedance, relative association, and intermolecular free length. Furthermore, UV-Vis spectroscopy and cyclic voltammetry were employed to evaluate the binding constants and free energies of the drug-surfactant systems. These results provide key molecular insights into the thermodynamics of OFL partitioning and its binding mechanisms with amphiphilic assemblies. Such mechanistic understanding is crucial for the rational design of antibiotic delivery systems, facilitating precise control over drug loading and release dynamics in surfactant-based formulations.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"519-531"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144692248","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Design of a Multi-Epitope Vaccine using β-barrel Outer Membrane Proteins Identified in Chlamydia trachomatis. 利用沙眼衣原体β-桶状外膜蛋白设计多表位疫苗。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-09-04 DOI: 10.1007/s00232-025-00360-5

Amisha Panda, Jahnvi Kapoor, B Hareramadas, Ilmas Naqvi, Satish Ganta, Ravindresh Chhabra, Sanjiv Kumar, Anannya Bandyopadhyay

{"title":"Design of a Multi-Epitope Vaccine using β-barrel Outer Membrane Proteins Identified in Chlamydia trachomatis.","authors":"Amisha Panda, Jahnvi Kapoor, B Hareramadas, Ilmas Naqvi, Satish Ganta, Ravindresh Chhabra, Sanjiv Kumar, Anannya Bandyopadhyay","doi":"10.1007/s00232-025-00360-5","DOIUrl":"10.1007/s00232-025-00360-5","url":null,"abstract":"Chlamydia trachomatis is an obligate intracellular Gram-negative pathogen that causes sexually transmitted infections (STIs) and trachoma. Current interventions are limited due to the widespread nature of asymptomatic infections, and the absence of a licensed vaccine exacerbates the challenge. In this study, we predicted outer membrane β-barrel (OMBB) proteins and designed a multi-epitope vaccine (MEV) construct using identified proteins. We employed a consensus-based computational framework on the C. trachomatis D/UW-3/CX proteome and identified 17 OMBB proteins, including well-known Pmp family members and MOMP. Eight OMBB proteins were computationally characterized, showing significant structural homology with known outer membrane proteins from other bacteria. Sequence-based annotation tools were used to determine their putative functions. B-cell and T-cell epitopes were predicted from the selected proteins. The MEV construct was designed using four cytotoxic T-lymphocyte (CTL) epitopes and 29 helper T-lymphocyte (HTL) epitopes from six OMBB proteins, which were conserved across 106 C. trachomatis serovars. To enhance its immunogenicity, the vaccine was supplemented with the Cholera toxin B subunit and PADRE sequence at the N-terminus. The MEV construct, of length 780 amino acids, was predicted to be antigenic, non-allergenic, non-toxic, and soluble. Secondary structure analysis revealed 95% random coils. A three-dimensional structural model of the MEV was generated and subsequently validated. Molecular docking between MEV and toll-like receptor 4 (TLR4) revealed strong and stable binding interactions. The MEV-TLR4 complex was found to be structurally compact and stable using molecular dynamics simulation. Immune simulation of the MEV construct elicited a strong immune response. This study highlights OMBB proteins as promising immunogenic targets and presents a computationally designed MEV candidate for C. trachomatis infection.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"473-496"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144994128","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Predicting Toxicity of Insect Venom-Derived Antimicrobial Peptides Using MD Simulations: A Comparative Study of Multi-Component and Realistic Mammalian Membrane Models. 用MD模拟预测昆虫毒液来源的抗菌肽的毒性：多组分和真实哺乳动物膜模型的比较研究。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-10-18 DOI: 10.1007/s00232-025-00363-2

P Chandra Sekar, Ulka Gawde, Chandan Kumar, Susan Idicula-Thomas

{"title":"Predicting Toxicity of Insect Venom-Derived Antimicrobial Peptides Using MD Simulations: A Comparative Study of Multi-Component and Realistic Mammalian Membrane Models.","authors":"P Chandra Sekar, Ulka Gawde, Chandan Kumar, Susan Idicula-Thomas","doi":"10.1007/s00232-025-00363-2","DOIUrl":"10.1007/s00232-025-00363-2","url":null,"abstract":"Insect venom-derived antimicrobial peptides (AMPs) hold significant therapeutic promise, but their application is constrained by mammalian cell toxicity. Toxicity assays are rapid and high-throughput, but screening large peptide libraries remains resource-intensive due to the requirements for peptide synthesis, purification, and testing. Alternatively, molecular dynamics (MD) simulations using mammalian membrane models provide an efficient and robust method for preliminary toxicity prediction. To benchmark the optimal model, two distinct mammalian membrane systems with diverse lipid compositions were evaluated for a set of sixteen toxic and fourteen non-toxic AMP analogs from five distinct insect AMP families, viz. anoplin, polybia, halictine, hyline, and macropin. In this study, a total of 25 µs of MD simulation time was generated. The analysis of MD trajectories, each spanning 500 ns for each of the 30 peptides, revealed significant variations in structural stability and membrane permeability between toxic and non-toxic AMPs, which aligned with the experimental results. Root Mean Square Deviation (RMSD) of the peptides during the last 100 ns of the simulation period successfully distinguished toxic from non-toxic AMPs with 90% accuracy when using realistic membrane models. The well-cited multicomponent mammalian membrane model failed to effectively predict mammalian toxicity. These findings underscore the efficacy of MD simulations in predicting the toxicity of venom-derived AMPs, thereby opening avenues for the accelerated development of safer antimicrobial therapies.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"505-518"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Celebrating 50 Years of Single-Channel Recording with the Patch Clamp. 用膜片钳庆祝单通道录音50周年。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-09-19 DOI: 10.1007/s00232-025-00362-3

Luigi Catacuzzeno, Fabio Franciolini

引用次数: 0

Yeast Membrane Hydration is Maintained Under Ethanol Exposure. 酵母膜水合作用在乙醇暴露下维持。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-09-15 DOI: 10.1007/s00232-025-00359-y

Dario M Genovese, Facundo L Scarzello, Georgina M Domini, Matías Crosio, Paulo B Miranda, Natalia Wilke

{"title":"Yeast Membrane Hydration is Maintained Under Ethanol Exposure.","authors":"Dario M Genovese, Facundo L Scarzello, Georgina M Domini, Matías Crosio, Paulo B Miranda, Natalia Wilke","doi":"10.1007/s00232-025-00359-y","DOIUrl":"10.1007/s00232-025-00359-y","url":null,"abstract":"Yeasts are able to tolerate different environmental conditions, including stress situations. Given their broad applications in the food industry, their ability to adapt to stressful conditions is an active area of research. Lipid composition of the yeast membrane is affected by environmental stress, and thus, the regulation of the membrane biophysical properties under such conditions may be a key point for yeast adaptation. Although Saccharomyces cerevisiae is highly tolerant to ethanol, its growth is inhibited when this alcohol accumulates in the medium. Therefore, we studied the effect of ethanol on yeast membranes using the fluorescent probe Laurdan, which is sensitive to water dipolar relaxation. Three strains were used: a laboratory strain of S. cerevisiae (BY4741), a mutant that lacks ergosterol (erg6 <math><mi>Δ</mi></math> ), and a commercial baker's yeast. At low ethanol levels, the emission signal of the probe remained constant for all strains. For ethanol proportions higher than 20% (v/v), at which cells are no longer viable, the signal changed abruptly, indicating an increase in solvent dipolar relaxation. We further studied BY4741 yeasts acclimated to high ethanol levels and found that water was more ordered in these membranes than in BY4741 grown in the absence of ethanol. We propose that water structure and membrane hydration are key for yeast viability in the presence of ethanol, and that studying the biophysical properties of membranes could be useful to identify yeast strains with a high tolerance to ethanol.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"447-460"},"PeriodicalIF":2.9,"publicationDate":"2025-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bilayer-Dependent Recognition of Docosahexaenoic Acid by the Transmembrane Domain of FATP3. FATP3跨膜结构域对二十二碳六烯酸的双层依赖性识别。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-12-01 Epub Date: 2025-09-15 DOI: 10.1007/s00232-025-00361-4

Yi Ding, Yonghua Wang, Wen Chen

引用次数: 0

Stomatin and Stomatin-Like Proteins Can Regulate Transporter Proteins Activity and Has a Role in Cancer Metastasis. 口蛋白和口蛋白样蛋白可调节转运蛋白活性并在肿瘤转移中起作用。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-07-10 DOI: 10.1007/s00232-025-00355-2

Abira Dey, Debabani Ganguly

{"title":"Stomatin and Stomatin-Like Proteins Can Regulate Transporter Proteins Activity and Has a Role in Cancer Metastasis.","authors":"Abira Dey, Debabani Ganguly","doi":"10.1007/s00232-025-00355-2","DOIUrl":"10.1007/s00232-025-00355-2","url":null,"abstract":"Stomatin, encoded by STOM gene, is an integral membrane protein found in a wide variety of species. Although years have passed since the identification of stomatin, little has been known about the functional insights of stomatin among which stomatin undergoing homo-oligomerization, post and reverse-post modifications are the notable ones. Stomatin downregulation or overexpression is directly connected to its ability to control neutrophil degranulation, modulate activities of transporter proteins, and mediate cancer metastasis. Stomatin shares about 40-80% sequence similarity at its signature SPFH (stomatin, prohibitin, flotillin and Hlfk) domain region with the stomatin-like proteins (SLPs). Although stomatin and SLPs are reported to have various therapeutic activities, still gaps are there regarding their plausible mechanistic insights. Therefore, in future, studies should be aimed toward investigating the possible mechanistic pathways controlled by stomatin and SLPs which can be employed to understand the basis of many therapeutic targets. This review briefs about the different functions of stomatin focusing mainly on the transporter proteins and carcinogenicity modulation by stomatin and SLPs.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"391-401"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0