Journal of Membrane Biology最新文献

Predicting Toxicity of Insect Venom-Derived Antimicrobial Peptides Using MD Simulations: A Comparative Study of Multi-Component and Realistic Mammalian Membrane Models. 用MD模拟预测昆虫毒液来源的抗菌肽的毒性：多组分和真实哺乳动物膜模型的比较研究。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-18 DOI: 10.1007/s00232-025-00363-2

P Chandra Sekar, Ulka Gawde, Chandan Kumar, Susan Idicula-Thomas

{"title":"Predicting Toxicity of Insect Venom-Derived Antimicrobial Peptides Using MD Simulations: A Comparative Study of Multi-Component and Realistic Mammalian Membrane Models.","authors":"P Chandra Sekar, Ulka Gawde, Chandan Kumar, Susan Idicula-Thomas","doi":"10.1007/s00232-025-00363-2","DOIUrl":"https://doi.org/10.1007/s00232-025-00363-2","url":null,"abstract":"Insect venom-derived antimicrobial peptides (AMPs) hold significant therapeutic promise, but their application is constrained by mammalian cell toxicity. Toxicity assays are rapid and high-throughput, but screening large peptide libraries remains resource-intensive due to the requirements for peptide synthesis, purification, and testing. Alternatively, molecular dynamics (MD) simulations using mammalian membrane models provide an efficient and robust method for preliminary toxicity prediction. To benchmark the optimal model, two distinct mammalian membrane systems with diverse lipid compositions were evaluated for a set of sixteen toxic and fourteen non-toxic AMP analogs from five distinct insect AMP families, viz. anoplin, polybia, halictine, hyline, and macropin. In this study, a total of 25 µs of MD simulation time was generated. The analysis of MD trajectories, each spanning 500 ns for each of the 30 peptides, revealed significant variations in structural stability and membrane permeability between toxic and non-toxic AMPs, which aligned with the experimental results. Root Mean Square Deviation (RMSD) of the peptides during the last 100 ns of the simulation period successfully distinguished toxic from non-toxic AMPs with 90% accuracy when using realistic membrane models. The well-cited multicomponent mammalian membrane model failed to effectively predict mammalian toxicity. These findings underscore the efficacy of MD simulations in predicting the toxicity of venom-derived AMPs, thereby opening avenues for the accelerated development of safer antimicrobial therapies.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-10-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145314123","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Stomatin and Stomatin-Like Proteins Can Regulate Transporter Proteins Activity and Has a Role in Cancer Metastasis. 口蛋白和口蛋白样蛋白可调节转运蛋白活性并在肿瘤转移中起作用。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-07-10 DOI: 10.1007/s00232-025-00355-2

Abira Dey, Debabani Ganguly

{"title":"Stomatin and Stomatin-Like Proteins Can Regulate Transporter Proteins Activity and Has a Role in Cancer Metastasis.","authors":"Abira Dey, Debabani Ganguly","doi":"10.1007/s00232-025-00355-2","DOIUrl":"10.1007/s00232-025-00355-2","url":null,"abstract":"Stomatin, encoded by STOM gene, is an integral membrane protein found in a wide variety of species. Although years have passed since the identification of stomatin, little has been known about the functional insights of stomatin among which stomatin undergoing homo-oligomerization, post and reverse-post modifications are the notable ones. Stomatin downregulation or overexpression is directly connected to its ability to control neutrophil degranulation, modulate activities of transporter proteins, and mediate cancer metastasis. Stomatin shares about 40-80% sequence similarity at its signature SPFH (stomatin, prohibitin, flotillin and Hlfk) domain region with the stomatin-like proteins (SLPs). Although stomatin and SLPs are reported to have various therapeutic activities, still gaps are there regarding their plausible mechanistic insights. Therefore, in future, studies should be aimed toward investigating the possible mechanistic pathways controlled by stomatin and SLPs which can be employed to understand the basis of many therapeutic targets. This review briefs about the different functions of stomatin focusing mainly on the transporter proteins and carcinogenicity modulation by stomatin and SLPs.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"391-401"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144602149","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Biomimetic Bacterial Membrane Models for Comprehending Membrane Dynamics. 理解膜动力学的仿生细菌膜模型。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-06-25 DOI: 10.1007/s00232-025-00351-6

Harsha Bajaj

引用次数: 0

Resurrection of the Helical Hairpin Hypothesis for Understanding Coronavirus Fusion. 理解冠状病毒融合的螺旋发夹假说的复活。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-06-24 DOI: 10.1007/s00232-025-00350-7

Sahil Lall, M Vijayasarathy, N V Joshi, P Balaram

{"title":"Resurrection of the Helical Hairpin Hypothesis for Understanding Coronavirus Fusion.","authors":"Sahil Lall, M Vijayasarathy, N V Joshi, P Balaram","doi":"10.1007/s00232-025-00350-7","DOIUrl":"10.1007/s00232-025-00350-7","url":null,"abstract":"Coronaviruses use the spike protein (spike) to bind to target cells, and fuse the viral envelope with a host lipid membrane. Spike is a large trimeric surface glycoprotein, anchored to the viral membrane (envelope) by a single membrane-spanning polypeptide helix and a short intra-virion domain. In the SARS-CoV-2 virus, the spike is formed by three protomers of 1273 residues, each with two distinct domains separable by enzymatic proteolysis prior to infection. Thus far, enveloped virus surface glycoprotein structures have provided a detailed molecular view of the pre-fusion state, while structures of the post-fusion state have remained incomplete. The determination of the full-length structure of the SARS-CoV-2 spike in the post-fusion state is a landmark in furthering our understanding of the structural pre-requisites for membrane fusion. This perspective analyzes the fusion domain as revealed by the recent structure in the context of conserved sequences across diverse coronaviruses. We highlight the characterization of the membrane-embedded fusion peptide in a helical hairpin topology. This structure is discussed as a re-imagination of the helical hairpin hypothesis for polypeptide insertion into membranes, postulated by Engleman and Steitz over four decades ago.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"361-373"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144486811","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Mycobacterium tuberculosis TtfA is a Highly Stable Membrane-Anchored DNA-Binding Protein. 结核分枝杆菌TtfA是一种高度稳定的膜锚定dna结合蛋白。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-06-26 DOI: 10.1007/s00232-025-00352-5

Saksham Jain, Akanksha Gajanan Patil, Saniya Patil, Raju Mukherjee, Vikas Jain, Radhakrishnan Mahalakshmi

{"title":"Mycobacterium tuberculosis TtfA is a Highly Stable Membrane-Anchored DNA-Binding Protein.","authors":"Saksham Jain, Akanksha Gajanan Patil, Saniya Patil, Raju Mukherjee, Vikas Jain, Radhakrishnan Mahalakshmi","doi":"10.1007/s00232-025-00352-5","DOIUrl":"10.1007/s00232-025-00352-5","url":null,"abstract":"Mycobacterium tuberculosis (Mtb), the causative agent of tuberculosis (TB), is a deadly intracellular pathogen, with a persistent infectivity and high morbidity rate. Mtb has successfully evaded drugs and modern antibiotics, while also developing resistance and adaptability. To obtain newer avenues for therapeutics against Mtb, we proposed to identify and characterize membrane proteins of Mtb. To this end, we report the successful characterization of the locus rv0383c, which codes for a 284-residue membrane-anchored protein. We show that the protein product, named TtfA, possesses an N-terminal transmembrane helix, a low complexity region, an α + β central region, and a C-terminally highly unstructured region. Our studies reveal that the extramembranous domain possesses non-specific DNA-binding ability. Additionally, TtfA folds into a highly stable structure that resists thermal unfolding. TtfA is selectively sensitive to the surrounding pH. The promising outcomes we obtain with TtfA as one of the next-generation antibiotic targets against Mtb can pave the way for characterizing other membrane proteins toward finding long-term cures for this endemic disease.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"403-413"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144499041","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Passage of the Channel-Forming Agent Nystatin Through Ergosterol-Containing Lipid Membranes. 制霉菌素通过含麦角甾醇脂质膜的通道形成剂。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-07-07 DOI: 10.1007/s00232-025-00354-3

Megi Tinev, Luka Kristanc, Gregor Gomišček, Bojan Božič

引用次数: 0

Functional and Physiological Implications of Oligopeptide Transporters: Potential Targets for Pharmacological Interventions. 寡肽转运体的功能和生理意义：药物干预的潜在靶点。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-10-01 Epub Date: 2025-05-29 DOI: 10.1007/s00232-025-00348-1

Tapas Roy, Madhu Nath, Nabanita Halder, Rohit Saxena, Thirumurthy Velpandian

{"title":"Functional and Physiological Implications of Oligopeptide Transporters: Potential Targets for Pharmacological Interventions.","authors":"Tapas Roy, Madhu Nath, Nabanita Halder, Rohit Saxena, Thirumurthy Velpandian","doi":"10.1007/s00232-025-00348-1","DOIUrl":"10.1007/s00232-025-00348-1","url":null,"abstract":"Peptide transporters are important plasma membrane proteins that facilitate the cellular uptake of di- and tripeptides in addition to various peptidomimetic drugs. The proton-coupled oligopeptide transporter (POT) family consists of mainly four transporter proteins, which include two H+ -coupled oligopeptide transporters known as PEPT1 (SLC15A1) and PEPT2 (SLC15A2), along with two peptide/histidine transporters referred to as PHT1 (SLC15A4) and PHT2 (SLC15A3). These transporters play an important role in the drug delivery process in mammalian tissues. They are highly expressed in tissues such as the small intestine, kidney, liver, lung, and eye. PHT1 shows expression in immune cells, especially in B cells and plasmacytoid dendritic cells. The pathophysiological relevance of peptide transporters is emerging to be crucial in various disease conditions, e.g., PEPT1 plays a role in the physiopathology of the gastrointestinal system, particularly in IBD. Upregulated expression of peptide transporters has also been positively related to inflammatory responses. An increasing number of peptide-based drug therapies have been reported to have the potential for development of novel classes of drugs. For example, Carnosine (beta-alanyl-L-histidine) has been demonstrated to act as an antioxidant, antiglycating agent, and neuroprotector. It is transported by PEPT1 and PEPT2, facilitating its protective effects against oxidative stress in neurons and intestinal epithelial cells. Moreover, it has applications in the treatment of multidrug-resistant cancers and has been shown to improve glucose metabolism. This review gives an insight into the functional, physiological and pharmacological importance of proton-coupled oligopeptide transporter.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"375-390"},"PeriodicalIF":2.9,"publicationDate":"2025-10-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144182279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Celebrating 50 Years of Single-Channel Recording with the Patch Clamp. 用膜片钳庆祝单通道录音50周年。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-09-19 DOI: 10.1007/s00232-025-00362-3

Luigi Catacuzzeno, Fabio Franciolini

引用次数: 0

Bilayer-Dependent Recognition of Docosahexaenoic Acid by the Transmembrane Domain of FATP3. FATP3跨膜结构域对二十二碳六烯酸的双层依赖性识别。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-09-15 DOI: 10.1007/s00232-025-00361-4

Yi Ding, Yonghua Wang, Wen Chen

引用次数: 0

Yeast Membrane Hydration is Maintained Under Ethanol Exposure. 酵母膜水合作用在乙醇暴露下维持。

IF 2.9 4区生物学

Journal of Membrane Biology Pub Date : 2025-09-15 DOI: 10.1007/s00232-025-00359-y

Dario M Genovese, Facundo L Scarzello, Georgina M Domini, Matías Crosio, Paulo B Miranda, Natalia Wilke

{"title":"Yeast Membrane Hydration is Maintained Under Ethanol Exposure.","authors":"Dario M Genovese, Facundo L Scarzello, Georgina M Domini, Matías Crosio, Paulo B Miranda, Natalia Wilke","doi":"10.1007/s00232-025-00359-y","DOIUrl":"https://doi.org/10.1007/s00232-025-00359-y","url":null,"abstract":"Yeasts are able to tolerate different environmental conditions, including stress situations. Given their broad applications in the food industry, their ability to adapt to stressful conditions is an active area of research. Lipid composition of the yeast membrane is affected by environmental stress, and thus, the regulation of the membrane biophysical properties under such conditions may be a key point for yeast adaptation. Although Saccharomyces cerevisiae is highly tolerant to ethanol, its growth is inhibited when this alcohol accumulates in the medium. Therefore, we studied the effect of ethanol on yeast membranes using the fluorescent probe Laurdan, which is sensitive to water dipolar relaxation. Three strains were used: a laboratory strain of S. cerevisiae (BY4741), a mutant that lacks ergosterol (erg6 <math><mi>Δ</mi></math> ), and a commercial baker's yeast. At low ethanol levels, the emission signal of the probe remained constant for all strains. For ethanol proportions higher than 20% (v/v), at which cells are no longer viable, the signal changed abruptly, indicating an increase in solvent dipolar relaxation. We further studied BY4741 yeasts acclimated to high ethanol levels and found that water was more ordered in these membranes than in BY4741 grown in the absence of ethanol. We propose that water structure and membrane hydration are key for yeast viability in the presence of ethanol, and that studying the biophysical properties of membranes could be useful to identify yeast strains with a high tolerance to ethanol.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":""},"PeriodicalIF":2.9,"publicationDate":"2025-09-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145066287","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0