Passage of the Channel-Forming Agent Nystatin Through Ergosterol-Containing Lipid Membranes.

IF 2.3 4区生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY

Journal of Membrane Biology Pub Date : 2025-07-07 DOI:10.1007/s00232-025-00354-3

Megi Tinev, Luka Kristanc, Gregor Gomišček, Bojan Božič

引用次数: 0

Abstract

The passage of nystatin through the ergosterol-containing phospholipid bilayer was studied on monovesicular and multivesicular giant unilamellar vesicles (GUVs and MVVs). Phase-contrast optical microscopy was used to examine vesicles composed of a palmitoyl-oleoyl-phosphatidyl-choline (POPC) bilayer with either 15 or 45 mol% ergosterol. Three types of vesicles were analyzed: (i) GUVs, (ii) outer vesicles (outGUVs) of MVVs, and (iii) inner vesicles (inGUVs) of MVVs. The times of their ruptures were determined after their exposure to nystatin at concentrations of 250 and 500 $μ$ M. At both concentrations, the times that the inGUVs spent in the nystatin solution after the rupture of the corresponding outGUVs were significantly shorter than the rupture times of individual GUVs of the similar size. These differences in rupture times demonstrate that the ergosterol-containing POPC membrane is permeable to nystatin.

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制霉菌素通过含麦角甾醇脂质膜的通道形成剂。

在单泡和多泡巨型单层囊泡（GUVs和MVVs）上研究制霉菌素通过含麦角甾醇磷脂双分子层的过程。用相对比光学显微镜检查了棕榈酰油酰磷脂酰胆碱（POPC）双分子层与15%或45%麦角甾醇组成的囊泡。分析了三种类型的囊泡：(i) GUVs, (ii) MVVs的外囊泡（outGUVs）和（iii） MVVs的内囊泡（inGUVs）。在250 μ m和500 μ m的制霉菌素浓度下测定其破裂时间，在这两种浓度下，相应的输出guv在制霉菌素溶液中破裂的时间明显短于相同大小的单个guv的破裂时间。这些破裂时间的差异表明含麦角甾醇的POPC膜对制霉菌素是可渗透的。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Journal of Membrane Biology 生物-生化与分子生物学

CiteScore

4.80

自引率

4.20%

发文量

审稿时长

6-12 weeks

期刊介绍： The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.