Journal of Membrane Biology最新文献_第10页

Zeolite Based Pervaporation Membrane: A Review 沸石基渗透蒸发膜研究进展

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-30 DOI: 10.14579/membrane_journal.2022.32.6.383

J. Lee, R. Patel

引用次数: 0

Hydrogen Permeation Performance of Pd, Pd/Cu Membranes Manufactured through Electroless Plating 化学镀法制备Pd、Pd/Cu膜的氢渗透性能

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-30 DOI: 10.14579/membrane_journal.2022.32.6.456

Jeong In Lee, M. Shin, X. Zhuang, Jae Yeon Hwang, Chang-Hun Jeong, J. Park

引用次数: 0

Evaluation of Commercial Anion Exchange Membrane for the application to Water Electrolysis 商用阴离子交换膜在水电解中的应用评价

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-30 DOI: 10.14579/membrane_journal.2022.32.6.496

Jun Ho Park, Kwang Seop Im, S. Nam

引用次数: 0

Improved Copper Ion Recovery Efficiency through Surface Modification of Membranes in the Electrodialysis/Solvent Extraction Process 电渗析/溶剂萃取过程中膜表面改性提高铜离子回收效率

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-30 DOI: 10.14579/membrane_journal.2022.32.6.486

Joon-Won Park, R. Kim, Hyunju Lee, Min-seuk Kim, H. Sohn

引用次数: 0

Forces of Change: Optical Tweezers in Membrane Remodeling Studies. 变化的力量:膜重构研究中的光学镊子。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00241-1

Sudheer K Cheppali, Raviv Dharan, Raya Sorkin

{"title":"Forces of Change: Optical Tweezers in Membrane Remodeling Studies.","authors":"Sudheer K Cheppali, Raviv Dharan, Raya Sorkin","doi":"10.1007/s00232-022-00241-1","DOIUrl":"https://doi.org/10.1007/s00232-022-00241-1","url":null,"abstract":"Optical tweezers allow precise measurement of forces and distances with piconewton and nanometer precision, and have thus been instrumental in elucidating the mechanistic details of various biological processes. Some examples include the characterization of motor protein activity, studies of protein-DNA interactions, and characterizing protein folding trajectories. The use of optical tweezers (OT) to study membranes is, however, much less abundant. Here, we review biophysical studies of membranes that utilize optical tweezers, with emphasis on various assays that have been developed and their benefits and limitations. First, we discuss assays that employ membrane-coated beads, and overview protein-membrane interactions studies based on manipulation of such beads. We further overview a body of studies that make use of a very powerful experimental tool, the combination of OT, micropipette aspiration, and fluorescence microscopy, that allow detailed studies of membrane curvature generation and sensitivity. Finally, we describe studies focused on membrane fusion and fission. We then summarize the overall progress in the field and outline future directions.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10782216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 4

Micromechanics of Biomembranes. 生物膜的微观力学。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00254-w

T Bhatia

引用次数: 3

Lysine 101 in the CRAC Motif in Transmembrane Helix 2 Confers Cholesterol-Induced Thermal Stability to the Serotonin_1A Receptor. 跨膜螺旋2中CRAC基序中的赖氨酸101赋予5 -羟色胺1a受体胆固醇诱导的热稳定性。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00262-w

Parijat Sarkar, Akrati Bhat, Amitabha Chattopadhyay

{"title":"Lysine 101 in the CRAC Motif in Transmembrane Helix 2 Confers Cholesterol-Induced Thermal Stability to the Serotonin1A Receptor.","authors":"Parijat Sarkar, Akrati Bhat, Amitabha Chattopadhyay","doi":"10.1007/s00232-022-00262-w","DOIUrl":"https://doi.org/10.1007/s00232-022-00262-w","url":null,"abstract":"G protein-coupled receptors (GPCRs) constitute the largest class of membrane proteins that transduce signals across the plasma membrane and orchestrate a multitude of physiological processes within cells. The serotonin1A receptor is a crucial neurotransmitter receptor in the GPCR family involved in a multitude of neurological, behavioral and cognitive functions. We have previously shown, using a combination of experimental and simulation approaches, that membrane cholesterol acts as a key regulator of organization, dynamics, signaling and endocytosis of the serotonin1A receptor. In addition, we showed that membrane cholesterol stabilizes the serotonin1A receptor against thermal deactivation. In the present work, we explored the molecular basis of cholesterol-induced thermal stability of the serotonin1A receptor. For this, we explored the possible role of the K101 residue in a cholesterol recognition/interaction amino acid consensus (CRAC) motif in transmembrane helix 2 in conferring the thermal stability of the serotonin1A receptor. Our results show that a mutation in the K101 residue leads to loss in thermal stability of the serotonin1A receptor imparted by cholesterol, independent of membrane cholesterol content. We envision that our results could have potential implications in structural biological advancements of GPCRs and design of thermally stabilized receptors for drug development.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10765836","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

Lipid and Lipidation in Membrane Fusion. 膜融合中的脂质和脂化。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00267-5

Avijit Sardar, Nikesh Dewangan, Bishvanwesha Panda, Debosmita Bhowmick, Pradip K Tarafdar

{"title":"Lipid and Lipidation in Membrane Fusion.","authors":"Avijit Sardar, Nikesh Dewangan, Bishvanwesha Panda, Debosmita Bhowmick, Pradip K Tarafdar","doi":"10.1007/s00232-022-00267-5","DOIUrl":"https://doi.org/10.1007/s00232-022-00267-5","url":null,"abstract":"Membrane fusion plays a lead role in the transport of vesicles, neurotransmission, mitochondrial dynamics, and viral infection. There are fusion proteins that catalyze and regulate the fusion. Interestingly, various types of fusion proteins are present in nature and they possess diverse mechanisms of action. We have highlighted the importance of the functional domains of intracellular heterotypic fusion, homotypic endoplasmic reticulum (ER), homotypic mitochondrial, and type-I viral fusion. During intracellular heterotypic fusion, the SNAREs and four-helix bundle formation are prevalent. Type-I viral fusion is controlled by the membrane destabilizing properties of fusion peptide and six-helix bundle formation. The ER/mitochondrial homotypic fusion is controlled by GTPase activity and the membrane destabilization properties of the amphipathic helix(s). Although the mechanism of action of these fusion proteins is diverse, they have some similarities. In all cases, the lipid composition of the membrane greatly affects membrane fusion. Next, examples of lipidation of the fusion proteins were discussed. We suggest that the fatty acyl hydrophobic tail not only acts as an anchor but may also modulate the energetics of membrane fusion intermediates. Lipidation is also important to design more effective peptide-based fusion inhibitors. Together, we have shown that membrane lipid composition and lipidation are important to modulate membrane fusion.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9472184/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10421579","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 8

Remodeling of the Plasma Membrane by Surface-Bound Protein Monomers and Oligomers: The Critical Role of Intrinsically Disordered Regions. 表面结合蛋白单体和低聚物对质膜的重塑:内在无序区域的关键作用。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00256-8

Mussie K Araya, Yong Zhou, Alemayehu A Gorfe

{"title":"Remodeling of the Plasma Membrane by Surface-Bound Protein Monomers and Oligomers: The Critical Role of Intrinsically Disordered Regions.","authors":"Mussie K Araya, Yong Zhou, Alemayehu A Gorfe","doi":"10.1007/s00232-022-00256-8","DOIUrl":"https://doi.org/10.1007/s00232-022-00256-8","url":null,"abstract":"The plasma membrane (PM) of cells is a dynamic structure whose morphology and composition is in constant flux. PM morphologic changes are particularly relevant for the assembly and disassembly of signaling platforms involving surface-bound signaling proteins, as well as for many other mechanochemical processes that occur at the PM surface. Surface-bound membrane proteins (SBMP) require efficient association with the PM for their function, which is often achieved by the coordinated interactions of intrinsically disordered regions (IDRs) and globular domains with membrane lipids. This review focuses on the role of IDR-containing SBMPs in remodeling the composition and curvature of the PM. The ability of IDR-bearing SBMPs to remodel the Gaussian and mean curvature energies of the PM is intimately linked to their ability to sort subsets of phospholipids into nanoclusters. We therefore discuss how IDRs of many SBMPs encode lipid-binding specificity or facilitate cluster formation, both of which increase their membrane remodeling capacity, and how SBMP oligomers alter membrane shape by monolayer surface area expansion and molecular crowding.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC9718270/pdf/nihms-1851070.pdf","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10782718","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 5

Reconstitution of Fusion-Competent Human Placental Fusogen Syncytin-2. 人胎盘融合原合胞素-2的重组。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2022-12-01 DOI: 10.1007/s00232-022-00242-0

Lu Xu, Sha Sun

{"title":"Reconstitution of Fusion-Competent Human Placental Fusogen Syncytin-2.","authors":"Lu Xu, Sha Sun","doi":"10.1007/s00232-022-00242-0","DOIUrl":"https://doi.org/10.1007/s00232-022-00242-0","url":null,"abstract":"Mammalian placenta formation requires continuous fusion of trophoblasts. Human endogenous retrovirus-derived proteins syncytin-1 and syncytin-2 mediate cell-cell fusion of placental cytotrophoblasts to form syncytiotrophoblasts in primates, which is required for normal placenta function and fetal development. Syncytins are post-translationally cleaved by the endoprotease furin into surface (SU) and transmembrane (TM) subunits for activation. Little is currently known about the molecular mechanisms of syncytin-mediated cell-cell fusion, and their functions have not been well studied in vitro. Here, we express tagged syncytin-2 in mammalian HEK293T cells and demonstrate that the tagging greatly influences the cleavage and fusogenic activity of syncytin-2. By detecting the N-terminal tagged SU, we find that it is released into the extracellular space during the fusion process. Furthermore, when N-linked glycosylation and disulfide bond formation are blocked, the cleavage and fusogenic activity of syncytin-2 are inhibited. Finally, we were able to purify functional syncytin-2 from HEK293T cells and incorporate it into proteoliposomes. These findings lay a solid foundation for interogating the molecular mechanisms of syncytin-2-mediated cell-cell fusion in vitro.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2022-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10407987","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1