Journal of Membrane Biology最新文献_第3页

Potential Role of Nrf2, HER2, and ALDH in Cancer Stem Cells: A Narrative Review. Nrf2、HER2 和 ALDH 在癌症干细胞中的潜在作用：叙述性综述。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-02-14 DOI: 10.1007/s00232-024-00307-2

Azadeh Fakhrioliaei, Sepideh Tanhaei, SeyedAbbas Pakmehr, Maha Noori Shakir, Maytham T Qasim, Maryam Hariri, Alireza Nouhi Kararoudi, Mohammad Valilo

{"title":"Potential Role of Nrf2, HER2, and ALDH in Cancer Stem Cells: A Narrative Review.","authors":"Azadeh Fakhrioliaei, Sepideh Tanhaei, SeyedAbbas Pakmehr, Maha Noori Shakir, Maytham T Qasim, Maryam Hariri, Alireza Nouhi Kararoudi, Mohammad Valilo","doi":"10.1007/s00232-024-00307-2","DOIUrl":"10.1007/s00232-024-00307-2","url":null,"abstract":"Cancer is one of the main causes of death among humans, second only to cardiovascular diseases. In recent years, numerous studies have been conducted on the pathophysiology of cancer, and it has been established that this disease is developed by a group of stem cells known as cancer stem cells (CSCs). Thus, cancer is considered a stem cell disease; however, there is no comprehensive consensus about the characteristics of these cells. Several different signaling pathways including Notch, Hedgehog, transforming growth factor-β (TGF-β), and WNT/β-catenin pathways cause the self-renewal of CSCs. CSCs change their metabolic pathways in order to access easy energy. Therefore, one of the key objectives of researchers in cancer treatment is to destroy CSCs. Nuclear factor erythroid 2-related factor 2 (Nrf2) plays an essential role in the protection of CSCs from reactive oxygen species (ROS) and chemotherapeutic agents by regulating antioxidants and detoxification enzymes. Human epidermal growth factor receptor 2 (HER2) is a member of the tyrosine kinase receptor family, which contributes to the protection of cancer cells against treatment and implicated in the invasion, epithelial-mesenchymal transition (EMT), and tumorigenesis. Aldehyde dehydrogenases (ALDHs) are highly active in CSCs and protect the cells against damage caused by active aldehydes through the regulation of aldehyde metabolism. On the other hand, ALDHs promote the formation and maintenance of tumor cells and lead to drug resistance in tumors through the activation of various signaling pathways, such as the ALDH1A1/HIF-1α/VEGF axis and Wnt/β-catenin, as well as changing the intracellular pH value. Given the growing body of information in this field, in the present narrative review, we attempted to shed light on the function of Nrf2, HER2, and ALDH in CSCs.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"3-16"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139736557","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Quantal Properties of Voltage-Dependent Ca²⁺ Release in Frog Skeletal Muscle Persist After Reduction of [Ca²⁺] in the Sarcoplasmic Reticulum. 蛙骨骼肌中电压依赖性 Ca2+ 释放的数量特性在肉质网[Ca2+]减少后依然存在

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-03-09 DOI: 10.1007/s00232-024-00309-0

J F Olivera, G Pizarro

{"title":"Quantal Properties of Voltage-Dependent Ca2+ Release in Frog Skeletal Muscle Persist After Reduction of [Ca2+] in the Sarcoplasmic Reticulum.","authors":"J F Olivera, G Pizarro","doi":"10.1007/s00232-024-00309-0","DOIUrl":"10.1007/s00232-024-00309-0","url":null,"abstract":"In skeletal muscle, the Ca2+ release flux elicited by a voltage clamp pulse rises to an early peak that inactivates rapidly to a much lower steady level. Using a double pulse protocol the fast inactivation follows an arithmetic rule: if the conditioning depolarization is less than or equal to the test depolarization, then decay (peak minus steady level) in the conditioning release is approximately equal to suppression (unconditioned minus conditioned peak) of the test release. This is due to quantal activation by voltage, analogous to the quantal activation of IP3 receptor channels. Two mechanisms are possible. One is the existence of subsets of channels with different sensitivities to voltage. The other is that the clusters of Ca2+-gated Ryanodine Receptor (RyR) β in the parajunctional terminal cisternae might constitute the quantal units. These Ca2+-gated channels are activated by the release of Ca2+ through the voltage-gated RyR α channels. If the RyR β were at the basis of quantal release, it should be modified by strong inhibition of the primary voltage-gated release. This was attained in two ways, by sarcoplasmic reticulum (SR) Ca2+ depletion and by voltage-dependent inactivation. Both procedures reduced global Ca2+ release flux, but SR Ca2+ depletion reduced the single RyR current as well. The effect of both interventions on the quantal properties of Ca2+ release in frog skeletal muscle fibers were studied under voltage clamp. The quantal properties of release were preserved regardless of the inhibitory maneuver applied. These findings put a limit on the role of the Ca2+-activated component of release in generating quantal activation.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"37-50"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140068885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bioinformatic Study of Possible Acute Regulation of Acid Secretion in the Stomach. 胃酸分泌的可能急性调节的生物信息学研究

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-03-04 DOI: 10.1007/s00232-024-00310-7

Yan Hay Grace Lee, Nicole T Cerf, Nicholas Shalaby, Mónica R Montes, Ronald J Clarke

{"title":"Bioinformatic Study of Possible Acute Regulation of Acid Secretion in the Stomach.","authors":"Yan Hay Grace Lee, Nicole T Cerf, Nicholas Shalaby, Mónica R Montes, Ronald J Clarke","doi":"10.1007/s00232-024-00310-7","DOIUrl":"10.1007/s00232-024-00310-7","url":null,"abstract":"The gastric H+,K+-ATPase is an integral membrane protein which derives energy from the hydrolysis of ATP to transport H+ ions from the parietal cells of the gastric mucosa into the stomach in exchange for K+ ions. It is responsible for the acidic environment of the stomach, which is essential for digestion. Acid secretion is regulated by the recruitment of the H+,K+-ATPase from intracellular stores into the plasma membrane on the ingestion of food. The similar amino acid sequences of the lysine-rich N-termini α-subunits of the H+,K+- and Na+,K+-ATPases, suggests similar acute regulation mechanisms, specifically, an electrostatic switch mechanism involving an interaction of the N-terminal tail with the surface of the surrounding membrane and a modulation of the interaction via regulatory phosphorylation by protein kinases. From a consideration of sequence alignment of the H+,K+-ATPase and an analysis of its coevolution with protein kinase C and kinases of the Src family, the evidence points towards a phosphorylation of tyrosine-7 of the N-terminus by either Lck or Yes in all vertebrates except cartilaginous fish. The results obtained will guide and focus future experimental research.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"79-89"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11006737/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140023137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Streamlined Biotinylation, Enrichment and Analysis for Enhanced Plasma Membrane Protein Identification Using TurboID and TurboID-Start Biotin Ligases. 使用 TurboID 和 TurboID-Start 生物素连接酶简化生物素酰化、富集和分析，以加强质膜蛋白质鉴定。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-01-30 DOI: 10.1007/s00232-023-00303-y

Mehmet Sarihan, Murat Kasap, Gurler Akpinar

{"title":"Streamlined Biotinylation, Enrichment and Analysis for Enhanced Plasma Membrane Protein Identification Using TurboID and TurboID-Start Biotin Ligases.","authors":"Mehmet Sarihan, Murat Kasap, Gurler Akpinar","doi":"10.1007/s00232-023-00303-y","DOIUrl":"10.1007/s00232-023-00303-y","url":null,"abstract":"Plasma membrane proteins (PMPs) play pivotal roles in various cellular events and are crucial in disease pathogenesis, making their comprehensive characterization vital for biomedical research. However, the hydrophobic nature and low expression levels of PMPs pose challenges for conventional enrichment methods, hindering their identification and functional profiling. In this study, we presented a novel TurboID-based enrichment approach for PMPs that helped overcoming some of the existing limitations. We evaluated the efficacy of TurboID and its modified form, TurboID-START, in PMP enrichment, achieving efficient and targeted labelling of PMPs without the need for stable cell line generation. This approach resulted reduction in non-specific biotinylation events, leading to improved PMP enrichment and enabled assessment of the subcellular proteome associated with the plasma membrane. Our findings paved the way for studies targeting the dynamic nature of the plasma membrane proteome and aiming to capture transient associations of proteins with the plasma membrane. The novel TurboID-based enrichment approach presented here offers promising prospects for in-depth investigations into PMPs and their roles in cellular processes.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"91-105"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139576926","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Construction and Analysis of a Mitochondrial Metabolism-Related Prognostic Model for Breast Cancer to Evaluate Survival and Immunotherapy. 构建和分析与线粒体代谢相关的乳腺癌预后模型，以评估生存期和免疫疗法。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-03-05 DOI: 10.1007/s00232-024-00308-1

Yuting Lin, Zhongxin Huang, Baogen Zhang, Hanhui Yang, Shu Yang

{"title":"Construction and Analysis of a Mitochondrial Metabolism-Related Prognostic Model for Breast Cancer to Evaluate Survival and Immunotherapy.","authors":"Yuting Lin, Zhongxin Huang, Baogen Zhang, Hanhui Yang, Shu Yang","doi":"10.1007/s00232-024-00308-1","DOIUrl":"10.1007/s00232-024-00308-1","url":null,"abstract":"As one of the most prevalent malignancies among women, breast cancer (BC) is tightly linked to metabolic dysfunction. However, the correlation between mitochondrial metabolism-related genes (MMRGs) and BC remains unclear. The training and validation datasets for BC were obtained from The Cancer Genome Atlas and Gene Expression Omnibus databases, respectively. MMRG-related data were obtained from the Molecular Signatures Database. A risk score prognostic model incorporating MMRGs was established based on univariate, LASSO, and multivariate Cox regression analyses. Independent factors affecting BC prognosis were identified through regression analysis and presented in a nomogram. Single-sample gene set enrichment analysis was employed to assess the immune levels of high-risk (HR) and low-risk (LR) groups. The sensitivity of BC patients in the two groups to common anti-tumor drugs was evaluated by utilizing the Genomics of Drug Sensitivity in Cancer database. 12 MMRGs significantly associated with survival were selected from 1234 MMRGs. A 12-gene risk score prognostic model was built. In the multivariate regression analysis incorporating classical clinical factors, the MMRG-related risk score remained an independent prognostic factor. As revealed by tumor immune microenvironment analysis, the LR group with higher survival rates had elevated immune levels. The drug sensitivity results unmasked that the LR group demonstrated higher sensitivity to Irinotecan, Nilotinib, and Oxaliplatin, while the HR group demonstrated higher sensitivity to Lapatinib. The development of MMRG characteristics provides a comprehensive understanding of mitochondrial metabolism in BC, aiding in the prediction of prognosis and tumor microenvironment, and offering promising therapeutic choices for BC patients with different MMRG risk scores.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"63-78"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140029421","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lidocaine Inhibits Rat Prostate Cancer Cell Invasiveness and Voltage-Gated Sodium Channel Expression in Plasma Membrane. 利多卡因抑制大鼠前列腺癌细胞的侵袭性和浆膜中电压门控钠通道的表达

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-01-02 DOI: 10.1007/s00232-023-00302-z

Nahit Rizaner, Scott P Fraser, Ilknur Bugan Gul, Esma Purut, Mustafa B A Djamgoz, Seyhan Altun

{"title":"Lidocaine Inhibits Rat Prostate Cancer Cell Invasiveness and Voltage-Gated Sodium Channel Expression in Plasma Membrane.","authors":"Nahit Rizaner, Scott P Fraser, Ilknur Bugan Gul, Esma Purut, Mustafa B A Djamgoz, Seyhan Altun","doi":"10.1007/s00232-023-00302-z","DOIUrl":"10.1007/s00232-023-00302-z","url":null,"abstract":"There is increasing evidence, mostly from breast cancer, that use of local anaesthetics during surgery can inhibit disease recurrence by suppressing the motility of the cancer cells dependent on inherent voltage-gated sodium channels (VGSCs). Here, the possibility that lidocaine could affect cellular behaviours associated with metastasis was tested using the Dunning cell model of rat prostate cancer. Mostly, the strongly metastatic (VGSC-expressing) Mat-LyLu cells were used under both normoxic and hypoxic conditions. The weakly metastatic AT-2 cells served for comparison in some experiments. Lidocaine (1-500 μM) had no effect on cell viability or growth but suppressed Matrigel invasion dose dependently in both normoxia and hypoxia. Used as a control, tetrodotoxin produced similar effects. Exposure to hypoxia increased Nav1.7 mRNA expression but VGSCα protein level in plasma membrane was reduced. Lidocaine under both normoxia and hypoxia had no effect on Nav1.7 mRNA expression. VGSCα protein expression was suppressed by lidocaine under normoxia but no effect was seen in hypoxia. It is concluded that lidocaine can suppress prostate cancer invasiveness without effecting cellular growth or viability. Extended to the clinic, the results would suggest that use of lidocaine, and possibly other local anaesthetics, during surgery can suppress any tendency for post-operative progression of prostate cancer.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"17-24"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139075696","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Study of the Membrane Activity of the Synthetic Peptide ∆M3 Against Extended-Spectrum β-lactamase Escherichia coli Isolates. 合成肽 ∆M3 对广谱β-内酰胺酶大肠埃希菌分离物的膜活性研究。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-02-05 DOI: 10.1007/s00232-024-00306-3

Estefanía Fandiño-Devia, Gloria A Santa-González, Maria C Klaiss-Luna, Marcela Manrique-Moreno

{"title":"Study of the Membrane Activity of the Synthetic Peptide ∆M3 Against Extended-Spectrum β-lactamase Escherichia coli Isolates.","authors":"Estefanía Fandiño-Devia, Gloria A Santa-González, Maria C Klaiss-Luna, Marcela Manrique-Moreno","doi":"10.1007/s00232-024-00306-3","DOIUrl":"10.1007/s00232-024-00306-3","url":null,"abstract":"Escherichia coli is the most common microorganism causing nosocomial or community-acquired bacteremia, and extended-spectrum β-lactamase-producing Escherichia coli isolates are identified worldwide with increasing frequency. For this reason, it is necessary to evaluate potential new molecules like antimicrobial peptides. They are recognized for their biological potential which makes them promising candidates in the fight against infections. The goal of this research was to evaluate the potential of the synthetic peptide ΔM3 on several extended-spectrum β-lactamase producing E. coli isolates. The antimicrobial and cytotoxic activity of the peptide was spectrophotometrically determined. Additionally, the capacity of the peptide to interact with the bacterial membrane was monitored by fluorescence microscopy and infrared spectroscopy. The results demonstrated that the synthetic peptide is active against Escherichia coli isolates at concentrations similar to Meropenem. On the other hand, no cytotoxic effect was observed in HaCaT keratinocyte cells even at 10 times the minimal inhibitory concentration. Microscopy results showed a permeabilizing effect of the peptide on the bacteria. The infrared results showed that ΔM3 showed affinity for the lipids of the microorganism's membrane. The results suggest that the ∆M3 interacts with the negatively charged lipids from the E. coli by a disturbing effect on membrane. Finally, the secondary structure experiments of the peptide showed a random structure in solution that did not change during the interaction with the membranes.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"51-61"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"https://www.ncbi.nlm.nih.gov/pmc/articles/PMC11006780/pdf/","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139693382","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Identification of Prognostic and Immune Characteristics of Two Lung Adenocarcinoma Subtypes Based on TRPV Channel Family Genes. 基于 TRPV 通道家族基因鉴定两种肺腺癌亚型的预后和免疫特征

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2023-12-27 DOI: 10.1007/s00232-023-00300-1

Jianhua Jiang, Pengchao Zheng, Lei Li

{"title":"Identification of Prognostic and Immune Characteristics of Two Lung Adenocarcinoma Subtypes Based on TRPV Channel Family Genes.","authors":"Jianhua Jiang, Pengchao Zheng, Lei Li","doi":"10.1007/s00232-023-00300-1","DOIUrl":"10.1007/s00232-023-00300-1","url":null,"abstract":"Lung adenocarcinoma (LUAD) is one of the deadliest malignant tumors worldwide. Transient receptor potential vanilloid (TRPV) channels take pivotal parts in many cancers, but their impact on LUAD remains unexplored. In this study, LUAD samples were classified into two subtypes according to the expression characteristics of TRPV1-6 genes, with LUAD subtype cluster2 exhibiting significantly higher survival rates than cluster1. Subsequently, analysis of differentially expressed genes (DEGs) was performed between cluster1 and cluster2, revealing enrichment of DEGs in channel activity and Ca2+ signaling pathways. We established a protein-protein interaction network based on DEGs and constructed a LUAD prognostic model by using Cox regression analysis based on genes corresponding to 170 protein nodes. The prognostic model demonstrated good predictive ability for patient prognosis, with higher survival rates observed in the low-risk (LR) group. The risk score was validated as an independent prognostic indicator, according to Cox regression analysis. A clinically applicable nomogram was plotted. Immunological analysis indicated that the LR and high-risk (HR) groups had varied proportions of immune cell infiltration. The immunotherapy prediction indicated that LUAD patients in LR group had a greater likelihood to benefit from immune checkpoint blockade therapy. Furthermore, we hypothesized that the expression patterns of feature genes in the LUAD model were related to the sensitivity to lung cancer therapeutic drugs TAS-6417 and Erlotinib. To sum up, our LUAD prognostic model possessed clinical applicability for prognosis and immunotherapy response prediction.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"115-129"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139040829","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effects of Chemical Fixatives on Kinetic Measurements of Biomolecular Interaction on Cell Membrane. 化学固定剂对细胞膜上生物分子相互作用动力学测量的影响

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 Epub Date: 2024-01-11 DOI: 10.1007/s00232-024-00305-4

Tianbao Dong, Shengyang Wan, Yanhui Wang, Yaru Fu, Pengcheng Wang

{"title":"Effects of Chemical Fixatives on Kinetic Measurements of Biomolecular Interaction on Cell Membrane.","authors":"Tianbao Dong, Shengyang Wan, Yanhui Wang, Yaru Fu, Pengcheng Wang","doi":"10.1007/s00232-024-00305-4","DOIUrl":"10.1007/s00232-024-00305-4","url":null,"abstract":"Understanding the interaction between ligands and membrane proteins is important for drug design and optimization. Although investigation using live cells is desirable, it is not feasible in some circumstances and cell fixation is performed to reduce cell motion and degradation. This study compared the effects of five fixatives, i.e., formaldehyde vapor (FV), paraformaldehyde (PFA), acetone, methanol, and ethanol, on kinetic measurements via the LigandTracer method. We found that all five fixatives exerted insignificant effects on lectin-glycan interaction. However, antibody-receptor interaction is markedly perturbed by coagulant fixatives. The acetone fixation changed the binding of the anti-human epidermal growth factor receptor 2 (HER2) antibody to HER2 on the cell membrane from a 1:2 to a 1:1 binding model, while methanol and ethanol abolished the antibody binding possibly by removal of the HER2 receptors on the cell membrane. The capability of binding was retained when methanol fixation was performed at lower temperatures, albeit with a binding model of 1:1 instead. Moreover, whereas cell morphology does not exert a substantial impact on lectin-glycan interaction, it can indeed modify the binding model of antibody-receptor interaction. Our results provided insights into the selection of fixatives for cell-based kinetic studies.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":" ","pages":"131-142"},"PeriodicalIF":2.4,"publicationDate":"2024-04-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"139418385","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction: Role of Hydrophobic Amino-Acid Side-Chains in the Narrow Selectivity Filter of the CFTR Chloride Channel Pore in Conductance and Selectivity. 更正：疏水性氨基酸侧链在 CFTR 氯化物通道孔的窄选择性过滤器中对传导性和选择性的作用。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2024-04-01 DOI: 10.1007/s00232-023-00304-x

Paul Linsdell

引用次数: 0