Journal of Membrane Biology最新文献_第4页

Host Lipid Manipulation by Intracellular Bacteria: Moonlighting for Immune Evasion. 细胞内细菌对宿主脂质的操纵：免疫逃避的月光。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-11-08 DOI: 10.1007/s00232-023-00296-8

Naveen Challagundla, Deepti Phadnis, Aakriti Gupta, Reena Agrawal-Rajput

{"title":"Host Lipid Manipulation by Intracellular Bacteria: Moonlighting for Immune Evasion.","authors":"Naveen Challagundla, Deepti Phadnis, Aakriti Gupta, Reena Agrawal-Rajput","doi":"10.1007/s00232-023-00296-8","DOIUrl":"10.1007/s00232-023-00296-8","url":null,"abstract":"Lipids are complex organic molecules that fulfill energy demands and sometimes act as signaling molecules. They are mostly found in membranes, thus playing an important role in membrane trafficking and protecting the cell from external dangers. Based on the composition of the lipids, their fluidity and charge, their interaction with embedded proteins vary greatly. Bacteria can hijack host lipids to satisfy their energy needs or to conceal themselves from host cells. Intracellular bacteria continuously exploit host, from their entry into host cells utilizing host lipid machinery to exiting through the cells. This acquisition of lipids from host cells helps in their disguise mechanism. The current review explores various mechanisms employed by the intracellular bacteria to manipulate and acquire host lipids. It discusses their role in manipulating host membranes and the subsequence impact on the host cells. Modulating these lipids in macrophages not only serve the purpose of the pathogen but also modulates the macrophage energy metabolism and functional state. Additionally, we have explored the intricate pathogenic relationship and the potential prospects of using this knowledge in lipid-based therapeutics to disrupt pathogen dominance.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"71488203","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Track and Field of the Journal of Membrane Biology. 《膜生物学杂志》田径项目。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 DOI: 10.1007/s00232-023-00298-6

Alexey S Ladokhin

引用次数: 0

Correction: Hans Ussing Memorial Issue: Epithelial Membrane Transport. 更正:汉斯·乌辛纪念问题:上皮膜运输。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 DOI: 10.1007/s00232-023-00290-0

Stanley G Schultz, Alexander Leaf

引用次数: 0

Exploration of the Catalytic Cycle Dynamics of Vigna Radiata H⁺-Translocating Pyrophosphatases Through Hydrogen-Deuterium Exchange Mass Spectrometry. 氢-氘交换质谱法研究辐射薇纳H+-易位焦磷酸酶的催化循环动力学。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-11-13 DOI: 10.1007/s00232-023-00295-9

Li-Kun Huang, Yi-Cyuan Huang, Pin-Chuan Chen, Ching-Hung Lee, Shih-Ming Lin, Yuan-Hao Howard Hsu, Rong-Long Pan

{"title":"Exploration of the Catalytic Cycle Dynamics of Vigna Radiata H+-Translocating Pyrophosphatases Through Hydrogen-Deuterium Exchange Mass Spectrometry.","authors":"Li-Kun Huang, Yi-Cyuan Huang, Pin-Chuan Chen, Ching-Hung Lee, Shih-Ming Lin, Yuan-Hao Howard Hsu, Rong-Long Pan","doi":"10.1007/s00232-023-00295-9","DOIUrl":"10.1007/s00232-023-00295-9","url":null,"abstract":"Vigna radiata H+-translocating pyrophosphatases (VrH+-PPases, EC 3.6.1.1) are present in various endomembranes of plants, bacteria, archaea, and certain protozoa. They transport H+ into the lumen by hydrolyzing pyrophosphate, which is a by-product of many essential anabolic reactions. Although the crystal structure of H+-PPases has been elucidated, the H+ translocation mechanism of H+-PPases in the solution state remains unclear. In this study, we used hydrogen-deuterium exchange (HDX) coupled with mass spectrometry (MS) to investigate the dynamics of H+-PPases between the previously proposed R state (resting state, Apo form), I state (intermediate state, bound to a substrate analog), and T state (transient state, bound to inorganic phosphate). When hydrogen was replaced by proteins in deuterium oxide solution, the backbone hydrogen atoms, which were exchanged with deuterium, were identified through MS. Accordingly, we used deuterium uptake to examine the structural dynamics and conformational changes of H+-PPases in solution. In the highly conserved substrate binding and proton exit regions, HDX-MS revealed the existence of a compact conformation with deuterium exchange when H+-PPases were bound with a substrate analog and product. Thus, a novel working model was developed to elucidate the in situ catalytic mechanism of pyrophosphate hydrolysis and proton transport. In this model, a proton is released in the I state, and the TM5 inner wall serves as a proton piston.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Research Progress on TRPA1 in Diseases. TRPA1在疾病中的研究进展

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-04-11 DOI: 10.1007/s00232-023-00277-x

Jiajing Li, Hongfei Zhang, Qian Du, Junyu Gu, Jiangbo Wu, Qi Liu, Zhuo Li, Ting Zhang, Jingyu Xu, Rui Xie

引用次数: 0

Peptide Flexibility and the Hydrophobic Moment are Determinants to Evaluate the Clinical Potential of Magainins. 多肽柔韧性和疏水力矩是评价抗肽肽临床潜力的决定因素。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-04-25 DOI: 10.1007/s00232-023-00286-w

Daniel Balleza

{"title":"Peptide Flexibility and the Hydrophobic Moment are Determinants to Evaluate the Clinical Potential of Magainins.","authors":"Daniel Balleza","doi":"10.1007/s00232-023-00286-w","DOIUrl":"10.1007/s00232-023-00286-w","url":null,"abstract":"Using a flexibility prediction algorithm and in silico structural modeling, we have calculated the intrinsic flexibility of several magainin derivatives. In the case of magainin-2 (Mag-2) and magainin H2 (MAG-H2) we have found that MAG-2 is more flexible than its hydrophobic analog, Mag-H2. This affects the degree of bending of both peptides, with a kink around two central residues (R10, R11), whereas, in Mag-H2, W10 stiffens the peptide. Moreover, this increases the hydrophobic moment of Mag-H2, which could explain its propensity to form pores in POPC model membranes, which exhibit near-to-zero spontaneous curvatures. Likewise, the protective effect described in DOPC membranes for this peptide regarding its facilitation in pore formation would be related to the propensity of this lipid to form membranes with negative spontaneous curvature. The flexibility of another magainin analog (MSI-78) is even greater than that of Mag-2. This facilitates the peptide to present a kind of hinge around the central F12 as well as a C-terminal end prone to be disordered. Such characteristics are key to understanding the broad-spectrum antimicrobial actions exhibited by this peptide. These data reinforce the hypothesis on the determinant role of spontaneous membrane curvature, intrinsic peptide flexibility, and specific hydrophobic moment in assessing the bioactivity of membrane-active antimicrobial peptides.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9337467","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Role of Hydrophobic Amino-Acid Side-Chains in the Narrow Selectivity Filter of the CFTR Chloride Channel Pore in Conductance and Selectivity. 疏水性氨基酸侧链在CFTR氯化物通道孔的窄选择性过滤器中的导电性和选择性作用。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-10-12 DOI: 10.1007/s00232-023-00294-w

Paul Linsdell

{"title":"Role of Hydrophobic Amino-Acid Side-Chains in the Narrow Selectivity Filter of the CFTR Chloride Channel Pore in Conductance and Selectivity.","authors":"Paul Linsdell","doi":"10.1007/s00232-023-00294-w","DOIUrl":"10.1007/s00232-023-00294-w","url":null,"abstract":"Cystic fibrosis is caused by mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) anion channel. Structural analysis of CFTR has identified a narrow, hydrophobic region close to the extracellular end of the open channel pore that may function as a selectivity filter. The present study combines comprehensive mutagenesis of hydrophobic amino-acid side-chains within the selectivity filter with functional evaluation of channel Cl- conductance and anion selectivity. Among these hydrophobic amino-acids, one (F337) appears to play a dominant role in determining both conductance and selectivity. Anion selectivity appears to depend on both side-chain size and hydrophobicity at this position. In contrast, conductance is disrupted by all F337 mutations, suggesting that unique interactions between permeating Cl- ions and the native phenylalanine side-chain are important for conductance. Surprisingly, a positively charged lysine side-chain can be substituted for several hydrophobic residues within the selectivity filter (including F337) with only minor changes in pore function, arguing against a crucial role for overall hydrophobicity. These results suggest that localized interactions between permeating anions and amino-acid side-chains within the selectivity filter may be more important in determining pore functional properties than are global features such as overall hydrophobicity.","PeriodicalId":50129,"journal":{"name":"Journal of Membrane Biology","volume":null,"pages":null},"PeriodicalIF":2.4,"publicationDate":"2023-12-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41219009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"生物学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forces and Flows at Cell Surfaces. 细胞表面的力和流。

IF 2.3 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-09-29 DOI: 10.1007/s00232-023-00293-x

Aurelia R Honerkamp-Smith

引用次数: 0

The Effect of Benzyl Alcohol on the Voltage-Current Characteristics of Tethered Lipid Bilayers. 苯甲醇对脂质双层膜电压-电流特性的影响。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 Epub Date: 2023-09-20 DOI: 10.1007/s00232-023-00291-z

Hadeel Alobeedallah, Bruce Cornell, Mohammed Ghazal, Hans Coster

引用次数: 0

Correction to: Forces and Flows at Cell Surfaces. 更正：细胞表面的力和流。

IF 2.4 4区生物学

Journal of Membrane Biology Pub Date : 2023-12-01 DOI: 10.1007/s00232-023-00297-7

Aurelia R Honerkamp-Smith

引用次数: 0