胆固醇影响磷脂囊泡中由抗菌肽NK-2诱导的孔形成和膜-膜相互作用。

IF 2.3 4区 生物学 Q3 BIOCHEMISTRY & MOLECULAR BIOLOGY
Journal of Membrane Biology Pub Date : 2025-06-01 Epub Date: 2025-05-03 DOI:10.1007/s00232-025-00347-2
Surajit Das, Rajeev Jain, Kalyan Kumar Banerjee, Pabitra Maity, Krishnananda Chattopadhyay, Sanat Karmakar
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引用次数: 0

摘要

抗菌肽是先天免疫反应的一部分,通过形成孔,随后膜解体来显示其抗菌活性。膜中的胆固醇可以影响孔的形成过程,因为已知胆固醇会改变膜的渗透性和弹性。本研究系统探讨了胆固醇在调节抗菌肽NK-2与磷脂膜相互作用中的作用,以及NK-2在膜内诱导的孔形成过程。采用多种实验技术研究了不同胆固醇浓度的DOPC-DOPG和卵磷脂制备的大单层囊泡(LUVs)和大单层囊泡(GUVs)。本研究表明,在中等NK-2浓度下,随着胆固醇浓度的增加,zeta电位的大小和表面电荷密度都会降低。当暴露于NK-2时,含有胆固醇的luv的大小分布的增殖表明发生了囊泡聚集。guv的相衬显微照片以及在luv上进行的钙黄蛋白释放实验显示了气孔的存在。值得注意的是,发现胆固醇掺入膜中对膜的渗透性和孔隙形成过程的动力学都有显著影响。这项生物物理研究有助于了解胆固醇在影响膜抗菌功效方面的作用。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Cholesterol Affects the Pore Formation and the Membrane-Membrane Interaction Induced by an Antimicrobial Peptide, NK-2, in Phospholipid Vesicles.

Antimicrobial peptides are part of the innate immune response and show their antimicrobial activity by forming pores, followed by disintegration of the membrane. Cholesterol in the membrane can affect the pore formation process, as cholesterol is known to alter the permeability and elastic properties of the membrane. The present research systematically explores the role of cholesterol in modulating the interaction of the antimicrobial peptide NK-2 with phospholipid membranes, as well as the processes of pore formation induced by NK-2 within the membrane. Large unilamellar vesicles (LUVs) and giant unilamellar vesicles (GUVs) made from DOPC-DOPG and Egg PC with varying cholesterol concentrations have been studied using a variety of experimental techniques. The present study revealed that both the magnitude of zeta potential and surface charge density diminished as cholesterol concentrations increased at an intermediate NK-2 concentration. The proliferation of the size distributions of LUVs containing cholesterol when exposed to NK-2 indicates the occurrence of vesicle aggregation. The phase contrast micrographs of GUVs as well as the calcein release experiments on LUVs show evidence of pores. Notably, the incorporation of cholesterol into the membrane was found to have a significant effect on both the permeability of the membrane and the kinetics of the pore formation process. This biophysical research contributes essential knowledge regarding the role of cholesterol in influencing the antimicrobial efficacy of the membrane.

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来源期刊
Journal of Membrane Biology
Journal of Membrane Biology 生物-生化与分子生物学
CiteScore
4.80
自引率
4.20%
发文量
63
审稿时长
6-12 weeks
期刊介绍: The Journal of Membrane Biology is dedicated to publishing high-quality science related to membrane biology, biochemistry and biophysics. In particular, we welcome work that uses modern experimental or computational methods including but not limited to those with microscopy, diffraction, NMR, computer simulations, or biochemistry aimed at membrane associated or membrane embedded proteins or model membrane systems. These methods might be applied to study topics like membrane protein structure and function, membrane mediated or controlled signaling mechanisms, cell-cell communication via gap junctions, the behavior of proteins and lipids based on monolayer or bilayer systems, or genetic and regulatory mechanisms controlling membrane function. Research articles, short communications and reviews are all welcome. We also encourage authors to consider publishing ''negative'' results where experiments or simulations were well performed, but resulted in unusual or unexpected outcomes without obvious explanations. While we welcome connections to clinical studies, submissions that are primarily clinical in nature or that fail to make connections to the basic science issues of membrane structure, chemistry and function, are not appropriate for the journal. In a similar way, studies that are primarily descriptive and narratives of assays in a clinical or population study are best published in other journals. If you are not certain, it is entirely appropriate to write to us to inquire if your study is a good fit for the journal.
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