The Plant CellPub Date : 2025-07-01DOI: 10.1093/plcell/koaf136
Malcolm Bennett, Rahul Bhosale, Scott A Boden, Shu-Yan Chen, Tino Colombi, Toshiro Ito, Hongju Li, Poonam Mehra, Lars Østergaard, Meng Li, Liu Liu, Nana Otsuka, Bipin Pandey, Scott Poethig, Kalika Prasad, Yue Qu, Makoto Shirakawa, Yinghua Su, Cao Xu, Weicai Yang, Wenjie Zhang, Xiaolan Zhang, Xiansheng Zhang, Zhaoyang Zhou, Shuang Wu
{"title":"Developmental Pathways in Plants: Lessons from Arabidopsis for Crop Innovation","authors":"Malcolm Bennett, Rahul Bhosale, Scott A Boden, Shu-Yan Chen, Tino Colombi, Toshiro Ito, Hongju Li, Poonam Mehra, Lars Østergaard, Meng Li, Liu Liu, Nana Otsuka, Bipin Pandey, Scott Poethig, Kalika Prasad, Yue Qu, Makoto Shirakawa, Yinghua Su, Cao Xu, Weicai Yang, Wenjie Zhang, Xiaolan Zhang, Xiansheng Zhang, Zhaoyang Zhou, Shuang Wu","doi":"10.1093/plcell/koaf136","DOIUrl":"https://doi.org/10.1093/plcell/koaf136","url":null,"abstract":"The emergence of molecular biology, along with the use of Arabidopsis thaliana as a model organism, has significantly enhanced our understanding of plant development. Research on Arabidopsis has led to the identification of key regulatory genes involved in various developmental processes. In the past decade, advances in genome sequencing and the decoding of numerous plant genomes have enabled the application of these findings from Arabidopsis to crop species. In this review, leading plant scientists summarize historical insights gained from arabidopsis studies and highlight their implications for crop development, with the aim of inspiring further research in these promising new areas.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533079","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"A PHR transcription factor-directed gene network reveals key regulators of phosphate metabolism and starvation responses in tomato","authors":"Dongbo Lin, Peng Tian, Xiaoen Zhu, Zeteng Lin, Ziwei Li, Yongxia Zhang, Beixin Mo, Xuemei Chen, Tengbo Huang","doi":"10.1093/plcell/koaf171","DOIUrl":"https://doi.org/10.1093/plcell/koaf171","url":null,"abstract":"Phosphorus (P) is an essential nutrient for plants, and its scarcity, especially in the form of soluble phosphate (Pi), induces phosphate starvation responses (PSRs). Our research delves into the molecular mechanisms that regulate tomato (Solanum lycopersicum) plant adaptation to Pi deficiency, highlighting the role of two PHOSPHATE STARVATION RESPONSE (PHR) transcription factors, SlPHR3 and SlPHR4, as central regulators of Pi metabolism and related developmental/physiological processes, particularly PSRs. Notably, our investigation into the SlPHR3- and SlPHR4-regulated transcriptional network led to the discovery of three previously unidentified regulators of Pi metabolism and PSRs: SlGRAS47, SlBHLH48, and SlMYB28. We substantiated the important roles of SlMYB28 in mediating SlPHR3 and SlPHR4 function by demonstrating that SlMYB28 suppression reduces Pi and P accumulation, ameliorates PSR-related phenotypes, and decreases the expression of genes involved in Pi metabolism and PSRs in SlPHR3 and SlPHR4 overexpression plants. Our findings shed light on the adaptive strategies of plants to Pi starvation and open avenues for the identification of important genes involved in Pi metabolism and PSRs, which can be leveraged to improve Pi use efficiency in agricultural crops.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533080","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-07-01DOI: 10.1093/plcell/koaf170
Yaning Zhao, Hairong Liu, Jie Cao, Shuya Tan, Dawei Cheng, Shichun Li, Murao Zhang, Ruxue Zhang, Zhonghai Li
{"title":"FORKHEAD BOX1 promotes leaf senescence via the histone acetyltransferase HAC1 and the transcription factors TGA7 and ABF2/3","authors":"Yaning Zhao, Hairong Liu, Jie Cao, Shuya Tan, Dawei Cheng, Shichun Li, Murao Zhang, Ruxue Zhang, Zhonghai Li","doi":"10.1093/plcell/koaf170","DOIUrl":"https://doi.org/10.1093/plcell/koaf170","url":null,"abstract":"Leaf senescence is a tightly regulated developmental process influenced by complex genetic and epigenetic networks. Here, we identified AtFOX1, a previously uncharacterized forkhead box (FOX) protein in Arabidopsis (Arabidopsis thaliana), as a positive regulator of leaf senescence. Loss-of-function AtFOX1 mutants exhibit delayed senescence, whereas AtFOX1 overexpression accelerated this process. Mechanistically, AtFOX1 binds to the GTAAAA motif in the TGACG SEQUENCE-SPECIFIC BINDING PROTEIN 7 (TGA7) promoter, recruiting ARABIDOPSIS HISTONE ACETYLTRANSFERASE OF THE CBP FAMILY 1 (HAC1) to enhance histone H3 lysine 9 (H3K9) acetylation and promote TGA7 transcription. Genetic analyses demonstrated that AtFOX1-mediated senescence requires HAC1 function, while TGA7 acts downstream of HAC1. Furthermore, TGA7 directly activates ABA-RESPONSIVE ELEMENT BINDING FACTOR 2 (ABF2) and ABF3, key regulators of age- and ABA-induced leaf senescence. Disruption of ABF2/3/4 function mitigated the premature senescence phenotype resulting from TGA7 or AtFOX1 overexpression. Collectively, our findings reveal a AtFOX1-centered regulatory module controlling leaf senescence in Arabidopsis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144533077","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-29DOI: 10.1093/plcell/koaf145
Daniel Lüdke, Toshiyuki Sakai, Jiorgos Kourelis, AmirAli Toghani, Hiroaki Adachi, Andrés Posbeyikian, Raoul Frijters, Hsuan Pai, Adeline Harant, Juan Carlos Lopez-Agudelo, Bozeng Tang, Karin Ernst, Martin Ganal, Adriaan Verhage, Chih-Hang Wu, Sophien Kamoun
{"title":"A root-specific NLR network mediates immune signaling of resistance genes against plant parasitic nematodes","authors":"Daniel Lüdke, Toshiyuki Sakai, Jiorgos Kourelis, AmirAli Toghani, Hiroaki Adachi, Andrés Posbeyikian, Raoul Frijters, Hsuan Pai, Adeline Harant, Juan Carlos Lopez-Agudelo, Bozeng Tang, Karin Ernst, Martin Ganal, Adriaan Verhage, Chih-Hang Wu, Sophien Kamoun","doi":"10.1093/plcell/koaf145","DOIUrl":"https://doi.org/10.1093/plcell/koaf145","url":null,"abstract":"Plant nucleotide-binding domain and leucine-rich repeat immune receptors (NLRs) confer disease resistance to many foliar and root parasites. However, the extent to which NLR-mediated immunity is differentially regulated between plant organs is poorly known. Here, we show that a large cluster of tomato (Solanum lycopersicum) genes, encoding the cyst and root-knot nematode disease resistance proteins Hero and MeR1 as well as the NLR helper NLR required for cell death 6 (NRC6), is nearly exclusively expressed in the roots. This root-specific gene cluster emerged in Solanum species about 21 million years ago through gene duplication of the ancient asterid NRC network. NLR sensors in this gene cluster function exclusively through NRC6 helpers to trigger hypersensitive cell death. These findings indicate that the NRC6 gene cluster has sub-functionalized from the larger NRC network to specialize in mediating resistance against root pathogens, including cyst and root-knot nematodes. We propose that some NLR gene clusters and networks may have evolved organ-specific gene expression as an adaptation to particular parasites and to reduce the risk of autoimmunity.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"27 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144513136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-28DOI: 10.1093/plcell/koaf140
Adrienne H K Roeder, Yiting Shi, Shuhua Yang, Mohamad Abbas, Rashmi Sasidharan, Marcelo J Yanovsky, Jorge José Casal, Sandrine Ruffel, Nicolaus von Wirén, Sarah M Assmann, Noah A Kinscherf, Arkadipta Bakshi, Burcu Alptekin, Simon Gilroy, Malleshaiah SharathKumar, Salomé Prat, Cristiana T Argueso
{"title":"Translational Insights into Abiotic Interactions: From Arabidopsis to Crop Plants","authors":"Adrienne H K Roeder, Yiting Shi, Shuhua Yang, Mohamad Abbas, Rashmi Sasidharan, Marcelo J Yanovsky, Jorge José Casal, Sandrine Ruffel, Nicolaus von Wirén, Sarah M Assmann, Noah A Kinscherf, Arkadipta Bakshi, Burcu Alptekin, Simon Gilroy, Malleshaiah SharathKumar, Salomé Prat, Cristiana T Argueso","doi":"10.1093/plcell/koaf140","DOIUrl":"https://doi.org/10.1093/plcell/koaf140","url":null,"abstract":"Understanding crop plants responses to abiotic stress is increasingly important in this changing climate. We asked experts how discoveries in Arabidopsis thaliana have translated into advancements in abiotic crop stress resilience. The theme is that core regulatory networks identified in Arabidopsis are conserved in crops, but the molecular regulation varies among species. For cold tolerance, the regulatory framework is conserved, but MAP Kinase signaling promotes degradation of the INDUCER OF DREB1 EXPRESSION (ICE) transcription factor in Arabidopsis but inhibits it in rice. For hypoxia, manipulation of the oxygen sensing Arg/N-degron pathway discovered in Arabidopsis has improved waterlogging and flood tolerance in barley, maize, wheat, and soybean. For light signaling, overexpression of PHYTOCHROME B reduces shade avoidance, improving yield under dense planting in potato, soybean, and maize. In rice, understanding of nitrogen responsiveness, uptake, and transport in Arabidopsis has inspired engineering of the NRT1 nitrate transceptor to increase yield. Overexpressing Arabidopsis genes in crops confers drought tolerance, although none have been commercialized. Growing plants in space generates a complex array of stresses, and Arabidopsis experiments in the space station prepare for future development of robust crops as integral components of the life support systems. For environmental regulation of flowering time, the role of the GIGANTEA (GI) - CONTANS (CO) - FLOWERING LOCUS T (FT) module elucidated in Arabidopsis is largely conserved in crop plants, although additional regulators modify short day responsiveness in rice, soybean, chrysanthemum, and potato.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503734","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-28DOI: 10.1093/plcell/koaf162
Nicolas M Doll, Yannick Fierlej, Thomas Eekhout, Lisa Elias, Clément Bellot, Geng Sun, Carolin Grones, Stijn Aesaert, Griet Coussens, Riet De Rycke, Maria Šimášková, Emilie Montes, Chloé Plagnard, Peter M Rogowsky, Yemisrach Melkie Abebaw, Mohammed Bendahmane, Bert De Rybel, Laurens Pauwels, Thomas Widiez, Moritz K Nowack
{"title":"KIL transcription factors facilitate embryo growth in maize by promoting endosperm elimination via lytic cell death","authors":"Nicolas M Doll, Yannick Fierlej, Thomas Eekhout, Lisa Elias, Clément Bellot, Geng Sun, Carolin Grones, Stijn Aesaert, Griet Coussens, Riet De Rycke, Maria Šimášková, Emilie Montes, Chloé Plagnard, Peter M Rogowsky, Yemisrach Melkie Abebaw, Mohammed Bendahmane, Bert De Rybel, Laurens Pauwels, Thomas Widiez, Moritz K Nowack","doi":"10.1093/plcell/koaf162","DOIUrl":"https://doi.org/10.1093/plcell/koaf162","url":null,"abstract":"The endosperm is a transient nutritive tissue in plant seeds. During maize (Zea mays) grain development, two distinct endosperm cell death processes occur: in one process, the endosperm adjacent to the embryo scutellum (EAS) is completely dismantled; in the other, the starchy endosperm (SE) retains nutrient-packed cell corpses after grain filling. Here, we show that SE cell death degrades some organelles including the mitochondria and the endoplasmic reticulum, while preserving protein bodies, starch granules, and chromatin. In contrast, EAS cells undergo lytic cell death to remobilize stored nutrients through a complex corpse clearance process. Using single-cell transcriptome analysis, we identified two NAC transcription factors, KIRA-LIKE 1 (KIL1) and 2 (KIL2), as specifically upregulated in the EAS. Analyses using dominant and recessive loss-of-function kil mutants demonstrate that these genes redundantly promote cell death and corpse clearance in the EAS, but are not required for SE cell death. Reduced EAS cell death in kil loss-of-function mutants strongly impeded embryo growth, indicating that EAS elimination is crucial for optimal embryo development. Notably, kil1 and kil2 expression is regulated by DOSAGE-EFFECT DEFECTIVE1, an imprinted paternally expressed endosperm transcription factor. Our findings suggest paternal control over EAS cell death and the embryo-endosperm size ratio in maize, providing new leads to modulate this agronomically important trait.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503729","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-27DOI: 10.1093/plcell/koaf161
Li Liu, Xiangzhao Meng, Qinyi Ye, Da Guo, Yafei Zhao, Na Cao, Lihua Zheng, Fei Guo, Jiangqi Wen, Yiding Niu, Tao Wang, Jiangli Dong
{"title":"Regulation of the immunity-related VIK-APK-EDS1 pathway in Medicago for resistance to Phytophthora","authors":"Li Liu, Xiangzhao Meng, Qinyi Ye, Da Guo, Yafei Zhao, Na Cao, Lihua Zheng, Fei Guo, Jiangqi Wen, Yiding Niu, Tao Wang, Jiangli Dong","doi":"10.1093/plcell/koaf161","DOIUrl":"https://doi.org/10.1093/plcell/koaf161","url":null,"abstract":"Root rot, induced by Phytophthora medicaginis, causes devastating damage to perennial alfalfa (Medicago sativa). However, the mechanism by which P. medicaginis infects Medicago remains elusive. Here, we identified the VASCULAR HIGHWAY 1-INTERACTING KINASE (VIK)-ANKYRIN PROTEIN KINASE (APK)-ENHANCED DISEASE SUSCEPTIBILITY 1 (EDS1) pathway during P. medicaginis infection in Medicago truncatula. MtAPK is an autoimmune gene, and Mtapk-mediated autoimmunity depends on MtEDS1. P. medicaginis infection triggers MtVIK to phosphorylate Ser20 of MtAPK, enhancing the interaction between MtAPK and MtEDS1 in the cytoplasm and constraining the nuclear resistance of MtEDS1. Disease resistance could be enhanced not only by knocking out MtVIK but also by the Ser20Ala site mutation of MtAPK. Interestingly, we found that alfalfa germplasms with lower MsVIK expression after inoculation with P. medicaginis exhibited greater disease resistance. Furthermore, CRISPR/Cas9 editing of MsVIK mutants in alfalfa resulted in stronger disease resistance without growth or yield penalties. Taken together, VIK is a negative regulator of Medicago immunity and has significant potential for cultivating durable resistance in crops through genetic modification.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"249 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503361","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
{"title":"The evolutionary history and functional specialization of microRNA genes in Arabidopsis halleri and A. lyrata","authors":"Flavia Pavan, Jacinthe Azevedo Favory, Eléanore Lacoste, Chloé Beaumont, Firas Louis, Christelle Blassiau, Corinne Cruaud, Karine Labadie, Sophie Gallina, Mathieu Genete, Vinod Kumar, Ute Kramer, Rita A Batista, Claire Patiou, Laurence Debacker, Chloé Ponitzki, Esther Houzé, Eléonore Durand, Jean-Marc Aury, Vincent Castric, Sylvain Legrand","doi":"10.1093/plcell/koaf168","DOIUrl":"https://doi.org/10.1093/plcell/koaf168","url":null,"abstract":"MicroRNAs (miRNAs) are a class of small non-coding RNAs that play important regulatory roles in plant genomes. While some miRNA genes are deeply conserved, the majority appear to be species-specific, raising the question of how they emerge and integrate into cellular regulatory networks. To address this question, we first performed a detailed annotation of miRNA genes in the closely related Arabidopsis halleri and A. lyrata, then evaluated their phylogenetic conservation across 87 plant species. We then characterized the process by which newly emerged miRNA genes progressively acquire the properties of ‘canonical’ miRNA genes, in terms of size and stability of the hairpin precursor, loading of their cleavage products into Argonaute proteins, and potential to regulate downstream target genes. Analysis of nucleotide polymorphism distribution along the hairpin sequence (stem, mature miRNA, terminal loop) revealed that the selective constraints on recently emerged miRNA genes were initially weak, gradually increasing towards evolutionarily conserved miRNA genes. Our results illustrate the rapid birth-and-death of miRNA genes in plant genomes, and provide a detailed picture of the evolutionary progression toward canonical miRNAs by which a small fraction of de novo formed miRNA genes eventually integrate into ‘core’ biological processes.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"2 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503730","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-27DOI: 10.1093/plcell/koaf150
Thomas Dresselhaus, Martina Balboni, Lea Berg, Anika Dolata, Frank Hochholdinger, Yongyu Huang, Guojing Jiang, Maria von Korff, Jia-Chi Ku, Karina van der Linde, Jan Maika, Cecilia Lara Mondragon, Michael Raissig, Arp Schnittger, Thorsten Schnurbusch, Rüdiger Simon, Yvonne Stahl, Marja Timmermans, Venkatasubbu Thirulogachandar, Shuangshuang Zhao, Yaping Zhou
{"title":"How meristems shape plant architecture in cereals","authors":"Thomas Dresselhaus, Martina Balboni, Lea Berg, Anika Dolata, Frank Hochholdinger, Yongyu Huang, Guojing Jiang, Maria von Korff, Jia-Chi Ku, Karina van der Linde, Jan Maika, Cecilia Lara Mondragon, Michael Raissig, Arp Schnittger, Thorsten Schnurbusch, Rüdiger Simon, Yvonne Stahl, Marja Timmermans, Venkatasubbu Thirulogachandar, Shuangshuang Zhao, Yaping Zhou","doi":"10.1093/plcell/koaf150","DOIUrl":"https://doi.org/10.1093/plcell/koaf150","url":null,"abstract":"Meristems are major determinants of plant architecture, plant diversification and acclimation to environmental stresses. Moreover, meristems play also a major role during crop domestication and are fundamentally important for the productivity of crop plants as they directly determine biomass and grain yield. While vegetative meristems shape the basic plant body plan and produce all above- and below-ground parts of plants, some vegetative meristems transit to reproductive meristems forming sexual organs and germ cells. Most knowledge about plant meristems was generated using the model Arabidopsis. Compared to Arabidopsis, architecture of grasses or cereals including crops like maize, wheat, barley, rice and sorghum is more complex: cereals produce additional organs like a coleoptile, seminal roots originating from the scutellar nodes in the embryo and shoot-borne crown roots as well as highly complex inflorescence meristems with meristem types absent in eudicots. Moreover, studies in cereals indicated that paradigms based on studies using Arabidopsis are not universally applicable. This review therefore aims to provide a comprehensive overview about the initiation, establishment, maintenance and function of the various cereal meristems and their stem cell niches that shape our most important crop plants. Stem cell-like systems involved in leaf pattering and germline formation are also considered. A focus is also on the significant progress that has been made recently using novel tools to elucidate the gene regulatory networks (GRNs) underlying the development and function of the various cereal meristems.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503732","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
The Plant CellPub Date : 2025-06-27DOI: 10.1093/plcell/koaf166
Gabrielle C Buck, Ashley D Weeks, Niamh E Ordner, Bonnie Bartel
{"title":"Identifying and characterizing a missing peroxin—PEX8—in Arabidopsis thaliana","authors":"Gabrielle C Buck, Ashley D Weeks, Niamh E Ordner, Bonnie Bartel","doi":"10.1093/plcell/koaf166","DOIUrl":"https://doi.org/10.1093/plcell/koaf166","url":null,"abstract":"Peroxisomes are dynamic organelles that contribute to diverse metabolic functions, including β-oxidation, photorespiration, and phytohormone biosynthesis. Peroxisomes import proteins from the cytosol through the action of peroxins (PEX proteins), many of which are conserved among fungi, plants, and animals. An apparent exception is Pex8, which is essential for lumenal protein import in several yeast species but has not been reported outside of fungi. Here, we identified an uncharacterized Arabidopsis thaliana protein with predicted structural similarity to Saccharomyces cerevisiae Pex8. Like yeast Pex8, Arabidopsis PEX8 is primarily composed of predicted HEAT repeats and has two predicted peroxisome-targeting signals. pex8 insertional and frameshift mutations were lethal, whereas expressing an artificial microRNA targeting PEX8 impaired lumenal protein import into peroxisomes and conferred physiological defects indicative of peroxisome dysfunction. Fluorescent reporters fused to the N terminus of PEX8 localized within peroxisomes in puncta associated with peroxisomal membranes. Our data show that Arabidopsis PEX8 is functionally equivalent to yeast Pex8, revealing the conservation of peroxisomal protein import machinery across eukaryotes and raising the intriguing possibility that other “yeast-specific” peroxins have eluded discovery in plants and mammals because of low primary sequence conservation.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"70 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503731","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}
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