The Plant Cell最新文献_第3页

A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation. 一个简单的细胞周期控制系统为理解植物细胞增殖提供了一个框架。

The Plant Cell Pub Date : 2026-04-09 DOI: 10.1093/plcell/koag103

Facundo Romani,Ignacy Bonter,Marius Rebmann,Go Takahashi,Fernando Guzman-Chavez,Francesco De Batté,Yuki Hirakawa,Jim Haseloff

{"title":"A simple cell-cycle control system in Marchantia polymorpha provides a framework for understanding plant cell proliferation.","authors":"Facundo Romani,Ignacy Bonter,Marius Rebmann,Go Takahashi,Fernando Guzman-Chavez,Francesco De Batté,Yuki Hirakawa,Jim Haseloff","doi":"10.1093/plcell/koag103","DOIUrl":"https://doi.org/10.1093/plcell/koag103","url":null,"abstract":"Eukaryotic cell division is controlled by cyclins and cyclin-dependent kinases (CDKs). The high number of cyclin-CDK pairs in flowering plants hinders functional analysis due to redundancy and how this system might have worked in early land plant ancestors remains unresolved. Our phylogenetic analysis showed that non-seed plants have a simple system of cell cycle genes, suggesting that the complexity in seed plants is a derived feature. To explore simpler systems, we studied the liverwort Marchantia polymorpha, which possesses a reduced, non-redundant set of core cell-cycle genes. Single-cell RNA-seq and live imaging of fluorescent reporters revealed phase-specific expression of cell cycle genes during cell division, characterized by one predominant cyclin per phase in the vegetative gametophyte, with limited overlap at transitions. Live imaging of tagged cyclins indicated that protein turnover and localization contribute to phase specificity. Functional studies revealed that MpCYCD;1 is sufficient to promote cell cycle re-entry, while overexpression of MpCYCA and MpCYCB;1 causes growth arrest, consistent with their roles in the G1, S, and G2/M transitions. Our findings reveal conserved features of cell cycle control across eukaryotes and the ancestral state of land plants. Marchantia thus provides a powerful framework for understanding multicellular proliferation and its evolution, with the potential for engineering plant growth and development.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"131 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663784","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LIKE HETEROCHROMATIN PROTEIN 1: a Polycomb-beyond Composite Regulator of Chromatin in Development, Stress, and Defense LIKE异染色质蛋白1：发育、应激和防御中染色质的多梳复合调节因子

The Plant Cell Pub Date : 2026-04-07 DOI: 10.1093/plcell/koag101

Lingrui Zhang, Jian-Kang Zhu

引用次数: 0

Rhizobial auxin activates transcription factors to orchestrate YUC2-dependent auxin biosynthesis for soybean nodule development. 根瘤菌生长素激活转录因子调控依赖于yuc2的生长素生物合成，促进大豆根瘤发育。

The Plant Cell Pub Date : 2026-04-06 DOI: 10.1093/plcell/koag104

Chaofan Chen,Shengwei Wu,Yi Zhang,Huifang Xu,Jing Zhang,Xinyu Gong,Huilei Qiu,Bin Hu,Jiaomei Chen,Zheng Liang,Qiulin Liu,Tianli Tu,Zhen Gao,Laimei Huang,Fang Wang,Dawei Xin,Xu Chen

{"title":"Rhizobial auxin activates transcription factors to orchestrate YUC2-dependent auxin biosynthesis for soybean nodule development.","authors":"Chaofan Chen,Shengwei Wu,Yi Zhang,Huifang Xu,Jing Zhang,Xinyu Gong,Huilei Qiu,Bin Hu,Jiaomei Chen,Zheng Liang,Qiulin Liu,Tianli Tu,Zhen Gao,Laimei Huang,Fang Wang,Dawei Xin,Xu Chen","doi":"10.1093/plcell/koag104","DOIUrl":"https://doi.org/10.1093/plcell/koag104","url":null,"abstract":"Establishing the symbiosis between legumes and nitrogen-fixing rhizobia requires the precise modulation of auxin levels. However, our understanding of auxin's regulatory roles, particularly rhizobia-derived auxins, remains limited. Our study reveals that the auxin biosynthesis gene YUC2a is essential for the spatiotemporal control of nodule development in soybean (Glycine max). This process is orchestrated by three transcription factors: Nuclear Factor-YA9 (NF-YA9), Lateral Organ Boundaries Domain 41 (LBD41), and Nodule Inception 1a (NIN1a). In the early stages of nodulation, rhizobial auxin stimulates NF-YA9 expression, NF-YA9 then activates YUC2a expression in the cortical cell layer, establishing optimal auxin levels for nodule initiation. In the middle stages, rhizobial auxin elevates LBD41 expression, and LBD41 suppresses YUC2a to control auxin levels, ensuring proper rhizobia colonization. In the late stages, rhizobial auxin inhibits NIN1a expression, which increases YUC2a expression in nitrogen-fixing symbiosomes, fine-tuning optimal auxin levels for nodule maturation. Disruption of YUC2a and its homologs impairs cell division in nodule primordia, reducing nodule density and nitrogen fixation capacity. Conversely, cortex-specific overexpression of YUC2a promotes nodule formation but inhibits rhizobia colonization. This dynamic auxin regulation optimizes nodule development in soybean, revealing rhizobia-derived auxin's critical role in nitrogen-fixing symbiosis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147630263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FaNUDT19 contributes to 5' cap quality control by modulating NAD+ capped RNA dynamics during strawberry fruit ripening. FaNUDT19通过调节草莓果实成熟过程中NAD+封盖RNA的动态，参与5'帽质量控制。

The Plant Cell Pub Date : 2026-04-06 DOI: 10.1093/plcell/koag102

Quan Ma,Dong Li,Yicheng Ren,Diep R Ganguly,Hao Hu,Chenyang Huang,Jie Cao,Yanpei Chen,Jiaxing Tan,Brian D Gregory,Jianzhao Liu,Zisheng Luo

{"title":"FaNUDT19 contributes to 5' cap quality control by modulating NAD+ capped RNA dynamics during strawberry fruit ripening.","authors":"Quan Ma,Dong Li,Yicheng Ren,Diep R Ganguly,Hao Hu,Chenyang Huang,Jie Cao,Yanpei Chen,Jiaxing Tan,Brian D Gregory,Jianzhao Liu,Zisheng Luo","doi":"10.1093/plcell/koag102","DOIUrl":"https://doi.org/10.1093/plcell/koag102","url":null,"abstract":"Nicotinamide adenine dinucleotide (NAD+)-capped RNAs (NAD-RNAs) are widely present in prokaryotic and eukaryotic transcriptomes. However, their regulation during development remains unclear. Here, we investigated NAD-RNA regulation during receptacle ripening in strawberry (Fragaria × ananassa, Fa), a model for non-climacteric fruit ripening. We performed \"click reaction\"-based sequencing of NAD-RNAs (SPAAC-NAD-seq) to globally profile NAD-RNAs during ripening. This identified 9,109 NAD-RNAs produced from protein-coding genes (NAD-mRNAs), 6,479 from transposable elements (NAD-TE-RNAs), 4 from the mitochondrial genome, and 11 from the chloroplast genome. The number of NAD-RNAs tended to decrease, corresponding with lower abundance of the NAD+ cap during ripening. In addition, we characterized the function of the NAD-RNA scavenger Nudix (Nucleoside diphosphate linked moiety X)-Type Motif 19 (FaNUDT19). FaNUDT19 expression increased ∼3.5-fold during ripening. Biochemically, FaNUDT19 could hydrolyze multiple 5' caps, including NAD+, ATP, AMP, and m7G caps, with the strongest activity on NAD-RNAs. Indeed, FaNUDT19 overexpression in strawberry selectively affected a subset of NAD-mRNAs, allowing for the persistence of stable levels of ripening-related genes, such as PEROXIDASE (FaPOD). These results suggest that FaNUDT19 affects strawberry ripening by stabilizing mRNAs that produce proteins promoting coloration. Overall, our findings highlight the importance of dynamic NAD+ decapping by FaNUDT19 in strawberry ripening.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147630259","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The TERMINAL FLOWER1-NODULE ROOTs-FRUITFULLc module orchestrates compound inflorescence development in Medicago truncatula. 末端花1-根瘤根-结果模块协调了短梗紫花苜蓿复合花序的发育。

The Plant Cell Pub Date : 2026-04-04 DOI: 10.1093/plcell/koag099

Juanjuan Zhang,Xiao Wang,Jiaqi Zhao,Lu Han,Feng Yuan,Jing Zhang,Kirankumar S Mysore,Jiangqi Wen,Chuanen Zhou

{"title":"The TERMINAL FLOWER1-NODULE ROOTs-FRUITFULLc module orchestrates compound inflorescence development in Medicago truncatula.","authors":"Juanjuan Zhang,Xiao Wang,Jiaqi Zhao,Lu Han,Feng Yuan,Jing Zhang,Kirankumar S Mysore,Jiangqi Wen,Chuanen Zhou","doi":"10.1093/plcell/koag099","DOIUrl":"https://doi.org/10.1093/plcell/koag099","url":null,"abstract":"Arresting the growth of inflorescence meristems causes a coordinated cessation of flowering that optimizes resource allocation for seed filling. Orthologs of Arabidopsis thaliana TERMINAL FLOWER1 (TFL1) regulate inflorescence meristem activity in various species, maintaining the proliferative phase and meristem indeterminacy. However, the mechanism regulating TFL1-mediated inflorescence meristem activity remains largely unknown, especially in species with compound inflorescences. Compound inflorescences are characterized by a secondary inflorescence (I2) produced by the primary inflorescence (I1) meristem, but the specific genes determining the number of I2 per node remain unclear. Here, we revealed that the arrest of the proliferative phase in Medicago truncatula is ensured through NODULE ROOTs (MtNOOTs)-mediated degradation of MtTFL1. Our results demonstrated that MtNOOTs repress the expression of FRUITFULLc (MtFULc), an I2 identity gene, thereby restricting the outgrowth of I2. We also showed that MtNOOTs-mediated protein turnover of MtTFL1 may contribute to this repression. MtTFL1 maintains I1 meristem activity and MtFULc controls I2 meristem outgrowth, whereas MtNOOTs limit these activities to control I1 meristem activity and restrict the outgrowth of the I2 meristem in the compound inflorescence of M. truncatula.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147617398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Functional insights into dispensable genes using genome-wide loss-of-function burden tests in Arabidopsis 在拟南芥中使用全基因组功能丧失负荷测试对可有可无基因的功能见解

The Plant Cell Pub Date : 2026-03-28 DOI: 10.1093/plcell/koag087

Kehan Zhao, Mariele Lensink, J Grey Monroe

{"title":"Functional insights into dispensable genes using genome-wide loss-of-function burden tests in Arabidopsis","authors":"Kehan Zhao, Mariele Lensink, J Grey Monroe","doi":"10.1093/plcell/koag087","DOIUrl":"https://doi.org/10.1093/plcell/koag087","url":null,"abstract":"Not all genes are essential for plant survival. With the rise of pan-genomics, it is evident that certain genes can be lost without negatively affecting fitness. Naturally occurring loss-of-function (LoF) mutations provide a valuable perspective on gene dispensability, offering insights into deleterious and adaptive gene loss. In this study, we identified 91,751 naturally occurring LoF variants from publicly available Arabidopsis genome data. Our findings demonstrate that LoF-intolerant genes are enriched in essential biological functions and associated with specific histone marks linked to active transcription. In contrast, LoF-tolerant genes exhibit relaxed selective pressure and are enriched in functions related to pollen rejection and defense responses, and can be used as a proxy for dispensable genes in the pan-genome. Using a random forest model trained on histone marks, we achieved moderate success in predicting gene LoF tolerance, with an area under the curve (AUC) of 0.718 in Arabidopsis and 0.767 in rice, and even across species. We also pioneered genome-wide LoF burden tests in Arabidopsis, collapsing independent LoF alleles into a single state to reduce allelic heterogeneity. By integrating LoF burden tests with transcriptomic data, we identified thousands of LoF-expression associations. Notably, this analysis accurately recapitulated the flowering time networks and identified FRIGIDA as a key regulator of flowering time genes. Furthermore, we found that collapsing alleles based on functional outcomes enhances association sensitivity. These results provide insight into gene dispensability and a framework for leveraging LoF mutations to study gene functions with improved association studies.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"44 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524338","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Connecting the nucleus to the tip: A protein module linking RSL4 transcription with ROP signaling in root hair growth. 连接细胞核到根尖：一个连接根毛生长中RSL4转录与ROP信号的蛋白质模块。

The Plant Cell Pub Date : 2026-03-27 DOI: 10.1093/plcell/koag097

Luciano M Di Fino

引用次数: 0

A dynamic phospho-switch that times diurnal stomatal movements in Arabidopsis. 拟南芥中调节气孔运动的动态磷开关。

The Plant Cell Pub Date : 2026-03-27 DOI: 10.1093/plcell/koag094

Xinyu Zhang

引用次数: 0

2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings 2-Cys过氧化物氧化酶和伴侣cpHSP70在拟南芥幼苗叶绿体生物发生中协同作用

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag096

Fernando Rodríguez-Marín, Antonia M Gallardo-Martínez, M Luisa Hernández, María-Cruz González, Francisco J Cejudo, Juan M Pérez-Ruiz

{"title":"2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings","authors":"Fernando Rodríguez-Marín, Antonia M Gallardo-Martínez, M Luisa Hernández, María-Cruz González, Francisco J Cejudo, Juan M Pérez-Ruiz","doi":"10.1093/plcell/koag096","DOIUrl":"https://doi.org/10.1093/plcell/koag096","url":null,"abstract":"The redox balance between NADPH-dependent thioredoxin reductase C (NTRC) and 2-Cys peroxiredoxins (PRXs) helps chloroplast photosynthetic performance acclimate rapidly to environmental cues. The Arabidopsis (Arabidopsis thaliana) 2cpab mutant, which lacks chloroplast 2-Cys PRXs A and B, shows impaired embryogenesis and cotyledon development. This phenotype indicates that these enzymes have a relevant function at early stages of plant development, although this function is poorly understood. Here, we show that the albino cotyledons of the 2cpab mutant have unstructured chloroplasts and decreased chloroplast lipid contents, revealing a key function of 2-Cys PRXs in cotyledon chloroplast differentiation. These phenotypes are mimicked by NTRC overexpression, whereas loss of NTRC function has no effect. RNA-Seq analyses showed transcriptomic changes resembling the response to proteotoxic stress in 2cpab seedlings, which was confirmed by the high sensitivity of 2cpab seedlings to heat stress. In de-etiolating seedlings 2-Cys PRXs interact with chloroplast-localized chaperone heat shock protein 70 (cpHSP70); moreover, Arabidopsis seedlings simultaneously lacking 2-Cys PRXs and cpHSP70-1 exhibit a dramatic alteration of seedling development. Based on these results, we propose that 2-Cys PRXs contribute to cotyledon chloroplast differentiation by affecting organellar proteostasis. This function is exerted in concert with chaperone cpHSP70 and is essential for seedling establishment.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNHL1 phase separation coordinates ethylene and gibberellin signaling to regulate wheat plant height. RNHL1相分离协调乙烯和赤霉素信号传导调节小麦株高。

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag092

Hongwei Jing

引用次数: 0