The Plant Cell最新文献_第2页

Setting the stage: The sleek cell-cycle machinery of the liverwort Marchantia polymorpha. 舞台布景：多形地茅（Marchantia polymorpha）光滑的细胞周期机制。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag111

Julie Robinson

引用次数: 0

A cytological framework of female meiosis in Arabidopsis. 拟南芥雌性减数分裂的细胞学框架。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag113

Bingyan Hu,Maria Ada Prusicki,Katharina Stahlmann,Yingqi Wang,Arp Schnittger

引用次数: 0

CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis. 拟南芥中CITF1与FIT相互作用并调控铜铁串扰。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag114

J C Chia,X Liu,S Dey,J Yan,Y Niu,O K Vatamaniuk

{"title":"CITF1 interacts with FIT and regulates copper-iron crosstalk in Arabidopsis.","authors":"J C Chia,X Liu,S Dey,J Yan,Y Niu,O K Vatamaniuk","doi":"10.1093/plcell/koag114","DOIUrl":"https://doi.org/10.1093/plcell/koag114","url":null,"abstract":"Iron (Fe) and copper (Cu) are essential yet potentially toxic metals with interconnected metabolic pathways; however, the mechanisms underlying Fe-Cu crosstalk remain poorly defined. Here, we show that CITF1 (COPPER DEFICIENCY INDUCED TRANSCRIPTION FACTOR 1), a Cu homeostasis regulator in Arabidopsis thaliana, physically interacts with FIT (FER-LIKE IRON DEFICIENCY-INDUCED TRANSCRIPTION FACTOR), the central Fe homeostasis regulator, forming a nutrient-responsive transcriptional module. Under Cu deficiency, the CITF1-FIT complex accumulates and promotes expression of the Cu uptake genes COPT2 (COPPER TRANSPORTER 2), FRO4 (FERRIC REDUCTION OXIDASE 4), and FRO5 (FERRIC REDUCTION OXIDASE 5). Proteasome-dependent degradation regulates CITF1 and FIT stability, with Cu deficiency delaying their turnover in a CITF1-dependent manner. Under Fe deficiency, CITF1 expression is downregulated, allowing FIT to interact with bHLH38/39/100/101 partners and activate Fe uptake genes, as CITF1 disrupts these interactions. Thus, CITF1 negatively regulates Fe acquisition. Consistent with this, citf1-1 and citf1-2 mutants show reduced sensitivity to Fe deficiency. Under Cu deficiency, the citf1-2 and fit-2 mutants have additive effects and under Fe deficiency, the double mutant shows partial suppression of the fit-2 slow growth phenotype, supporting the positive and negative roles of CITF1 in Cu and Fe homeostasis, respectively. Complete loss of CITF1 function in the homozygous citf1-1 fit-2 double mutant causes embryo lethality, revealing roles for CITF1 and FIT in embryo development. These findings establish CITF1 as a nutrient-responsive regulator of Cu/Fe crosstalk, functioning through interactions with FIT to prioritize Cu or Fe acquisition and balance micronutrient homeostasis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147695073","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Growth versus Decline: Root Aging and Plant Performance. 生长与衰退：根系老化与植物性能。

The Plant Cell Pub Date : 2026-04-16 DOI: 10.1093/plcell/koag115

Feng Qin,Wolfgang Busch

引用次数: 0

The LARGE3-OsHDT1 complex controls rice grain size and weight by modulating histone H4 acetylation of OsMKKK10. LARGE3-OsHDT1复合体通过调节OsMKKK10的组蛋白H4乙酰化来控制大米的粒度和重量。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag109

Guozheng Zhang,Junmin Wang,Xiaohui Jing,Sha Tang,Leiying Zheng,Linlin Zhang,Baolan Zhang,Ran Xu,Penggen Duan,Luojiang Huang,Ke Huang,Ying Gao,Limin Zhang,Jianqin Hao,Xianmin Diao,Yunhai Li

{"title":"The LARGE3-OsHDT1 complex controls rice grain size and weight by modulating histone H4 acetylation of OsMKKK10.","authors":"Guozheng Zhang,Junmin Wang,Xiaohui Jing,Sha Tang,Leiying Zheng,Linlin Zhang,Baolan Zhang,Ran Xu,Penggen Duan,Luojiang Huang,Ke Huang,Ying Gao,Limin Zhang,Jianqin Hao,Xianmin Diao,Yunhai Li","doi":"10.1093/plcell/koag109","DOIUrl":"https://doi.org/10.1093/plcell/koag109","url":null,"abstract":"Grain size is an important yield trait in cereal crops and understanding the mechanisms that control grain size is essential for precise breeding and improving cereal crop yields. Epigenetics plays a crucial role in plant growth and development, but how it is involved in grain size regulation remains largely unclear. Here we report that the histone-binding protein LARGE3 associates with the histone deacetylase OsHDT1 (HD-tuins 1) to control grain length by influencing histone H4 acetylation in rice (Oryza sativa). The large3 mutants form large and heavy grains because of increased cell proliferation. LARGE3 physically interacts with OsHDT1 to negatively regulate grain length. LARGE3 and OsHDT1 repress the expression of an important grain size gene OsMKKK10 by decreasing its histone H4 acetylation level and increasing its nucleosome density. Importantly, genome editing of LARGE3 homolog in foxtail millet (Setaria italica) causes large grains and increased grain yield. These findings reveal a molecular mechanism for LARGE3-OsHDT1 mediated epigenetic modulation of grain length by regulating histone H4 acetylation of OsMKKK10, suggesting that this module has potential applications in improving grain size and yield in key cereal crops.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"51 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heterodimers of the MADS transcription factors GhAGL1 and GhAGL4 modulate cotton fiber initiation and elongation. MADS转录因子GhAGL1和GhAGL4的异源二聚体调节棉纤维的起始和伸长。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag107

Mengling Sun,Yawen Xiao,Xiaxia Liu,Yuqi Liu,Menghui Zheng,Tian Lei,Yirong Tao,Weiran Wang,Chunyuan You,Zixin Zhou,Xianlong Zhang,Jie Kong,Lili Tu

{"title":"Heterodimers of the MADS transcription factors GhAGL1 and GhAGL4 modulate cotton fiber initiation and elongation.","authors":"Mengling Sun,Yawen Xiao,Xiaxia Liu,Yuqi Liu,Menghui Zheng,Tian Lei,Yirong Tao,Weiran Wang,Chunyuan You,Zixin Zhou,Xianlong Zhang,Jie Kong,Lili Tu","doi":"10.1093/plcell/koag107","DOIUrl":"https://doi.org/10.1093/plcell/koag107","url":null,"abstract":"The initial number of fibers and fiber length affect fiber yield and quality in cotton (Gossypium hirsutum). The mechanism of fiber initiation centers on the transcription factor GhMYB25-like, and the involvement of other regulatory factors remains unclear. Here, we established a GhMYB25-like-based interaction network that regulates fiber initiation, and focused on the interacting protein AGAMOUS-like 4 (GhAGL4), which is a MIKC-MADS transcription factor, and other MIKC-MADS family members. Genome-wide identification of the cotton MIKC-MADS family members identified two highly co-expressed genes, GhAGL1 and GhAGL4, which are predominantly expressed in ovules at 0 days post-anthesis (DPA). CRISPR/Cas9-mediated loss of function of GhAGL1 or GhAGL4 led to a decrease in fiber initials and impeded fiber elongation, with the Ghagl1 Ghagl4 double mutant exhibiting a stronger phenotype. GhAGL1 and GhAGL4 interact in vitro and in vivo. DNA affinity purification and sequencing (DAP-seq) combined with RNA sequencing identified CCCH zinc finger 1 (GhCZF1) as a downstream gene of GhAGL1 and GhAGL4. Interestingly, GhMYB25-like enhances the ability of GhAGL1-GhAGL4 heterodimers to activate downstream genes. During the early fiber elongation stage, GhCZF1 activates the expression of the xyloglucan endotransglycosylase/hydrolase (XTH) genes GhXTH16 and GhXTH23 to regulate fiber elongation. Our findings reveal a molecular mechanism involved in fiber initiation and early elongation, providing a foundation for enhancing cotton fiber yield and quality.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"279 3 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663782","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Orchestrating symbiosis: how bacterial auxin programs soybean nodulation. 协调共生：细菌生长素如何控制大豆结瘤。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag110

Ved Prakash

引用次数: 0

MdARF16/17 Link Auxin Signaling to Ma1-Independent Control of Vacuolar Malate Transport in Apple. MdARF16/17将生长素信号与苹果液泡中苹果酸转运的ma1独立控制联系起来。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag106

Chu-Kun Wang,Meng-Meng Wei,Fan Xiao,Yi-Tser Tsai,Xiao-Long Liu,Lian-Da Du,Yu-Wen Zhao,Xiao-Wen He,Peng-Liang Han,Hai-Bo Wang,Nan Wang,Mengxia Zhang,Sen Wang,Ting-Ting Zhao,Miguel A Piñeros,Lin-Guang Li,Xue-Sen Chen,Lailiang Cheng,Da-Gang Hu

{"title":"MdARF16/17 Link Auxin Signaling to Ma1-Independent Control of Vacuolar Malate Transport in Apple.","authors":"Chu-Kun Wang,Meng-Meng Wei,Fan Xiao,Yi-Tser Tsai,Xiao-Long Liu,Lian-Da Du,Yu-Wen Zhao,Xiao-Wen He,Peng-Liang Han,Hai-Bo Wang,Nan Wang,Mengxia Zhang,Sen Wang,Ting-Ting Zhao,Miguel A Piñeros,Lin-Guang Li,Xue-Sen Chen,Lailiang Cheng,Da-Gang Hu","doi":"10.1093/plcell/koag106","DOIUrl":"https://doi.org/10.1093/plcell/koag106","url":null,"abstract":"Auxin is a central plant hormone, and organic acids are key determinants of fruit acidity and flavor; however, the molecular mechanism connecting auxin signaling to organic acids remains elusive. Here, we report a negative correlation between auxin levels and malate, the predominant organic acid in apple (Malus domestica), in progenies of 'Gala' × 'Mato 1' and across fruit developmental stages. This suggests that auxin reduces malate levels independently of Ma1, an aluminum-activated malate transporter (ALMT) gene that is the causal gene for the major QTL determining fruit acidity in apple. Integrated bulked segregant analysis and RNA-seq identified MdARF16 and MdARF17 as auxin-responsive transcription factors that repress malate accumulation. Auxin represses two tonoplast-localized malate transporter genes, the ALMT gene MdALMT9L and the MdMATEL2 gene, which encodes a functionally distinct multidrug and toxic compound extrusion (MATE) protein. However, auxin does not repress Ma1. This repression requires the cooperative action of MdARF16 and MdARF17, which directly bind the promoters of the malate transporter genes to inhibit transcription. Decreased malate transporter levels then restrict cytosolic-to-vacuolar malate transport. These findings reveal a Ma1-independent auxin-MdARF16/17 pathway controlling vacuolar malate transport in apple, offering a molecular framework for the auxin-responsive improvement of organic acid traits.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663783","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Arabidopsis ARF3-AIP1/2-SAP18 module specifies the root stem cell niche in response to auxin. 拟南芥ARF3-AIP1/2-SAP18模块指定了响应生长素的根干细胞生态位。

The Plant Cell Pub Date : 2026-04-10 DOI: 10.1093/plcell/koag108

Zhi Juan Cheng,Meng Ru Zhang,Huan Kai Zhang,Xiao Li Chu,Bing Zhen Li,Miao Miao Zhang,Jia Yang Li,Wan Chen Dong,De Hao Wang,Wen Qi Xin,Xin Lin Han,Cai Yu Yu,Zhi Wei Wang,Xiao Hang Zhang,Jiong Hui Liu,Xian Sheng Zhang,Ya Lin Sang

{"title":"The Arabidopsis ARF3-AIP1/2-SAP18 module specifies the root stem cell niche in response to auxin.","authors":"Zhi Juan Cheng,Meng Ru Zhang,Huan Kai Zhang,Xiao Li Chu,Bing Zhen Li,Miao Miao Zhang,Jia Yang Li,Wan Chen Dong,De Hao Wang,Wen Qi Xin,Xin Lin Han,Cai Yu Yu,Zhi Wei Wang,Xiao Hang Zhang,Jiong Hui Liu,Xian Sheng Zhang,Ya Lin Sang","doi":"10.1093/plcell/koag108","DOIUrl":"https://doi.org/10.1093/plcell/koag108","url":null,"abstract":"In the root apical meristem, the stem cell niche (SCN) comprises a mitotically inactive quiescent center (QC) and adjacent, mitotically active stem cells that divide to form root tissues. Auxin dynamics are essential for specification and maintenance of the root SCN; however, the underlying mechanisms remain to be explored. Here, we report that Arabidopsis (Arabidopsis thaliana) AUXIN RESPONSE FACTOR3 (ARF3), ARF3-INTERACTING PROTEIN1/2 (AIP1/2) and SIN3-ASSOCIATED POLYPEPTIDE OF 18 KDA (SAP18) form a protein complex that specifies root SCN cell fate in response to auxin level. In cells proximal to the QC, the ARF3-AIP1/2-SAP18 complex bound the WOX5 promoter and restricted WOX5 expression to the QC by decreasing H3 histone acetylation, thereby maintaining the SCN. Disrupting the ARF3-AIP1/2-SAP18 complex via mutation or exposure to excessive amounts of auxin resulted in proximal and lateral expansion of WOX5 expression, and inhibited root elongation by repressing cell division. During de novo specification of the SCN in lateral root primordia or regenerating root tips, accumulated auxin caused the ARF3-AIP1/2-SAP18 complex to dissociate, allowing the induction of WOX5 expression. In the reestablished meristem, the ARF3-AIP1/2-SAP18 complex confines WOX5 expression to the newly formed QC. Our findings provide insights into the roles of auxin dynamics in determining root SCN.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"67 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-04-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147663780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Heat-induced Mutator: DNA repair polymerase Polλ shapes mutation under heat. 热诱导突变：DNA修复聚合酶Polλ在高温下形成突变。

The Plant Cell Pub Date : 2026-04-09 DOI: 10.1093/plcell/koag100

Satoyo Oya

引用次数: 0