The Plant Cell最新文献_第2页

Supersize me: A seed-specific protein facilitates lipid droplet enlargement in Arabidopsis. 超大型蛋白：一种种子特异性蛋白，可促进拟南芥脂滴增大。

The Plant Cell Pub Date : 2025-05-21 DOI: 10.1093/plcell/koaf131

Vicky Howe

引用次数: 0

KILing in the name of embryonic growth: KIL transcription factors drive cell death in the maize endosperm. 以胚胎生长为名的死亡：KIL转录因子驱动玉米胚乳细胞死亡。

The Plant Cell Pub Date : 2025-05-21 DOI: 10.1093/plcell/koaf133

Sonhita Chakraborty

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Quantitive disease resistance (QDR): The alternative to "all-or-nothing" strategy in plant immunity. 数量抗病性（QDR）：植物免疫“全有或全无”策略的替代品。

The Plant Cell Pub Date : 2025-05-21 DOI: 10.1093/plcell/koaf132

Pei Qin Ng

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Focus on Translational Research from Arabidopsis to Crop Plants and Beyond. 从拟南芥到农作物及其他植物的转化研究

The Plant Cell Pub Date : 2025-05-15 DOI: 10.1093/plcell/koaf119

Adrienne H K Roeder,Cristiana T Argueso,Mary Williams,Gabriela Auge,Xin Li,Lucia Strader,Cristobal Uauy,Shuang Wu

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LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings. 种子脂滴蛋白参与控制拟南芥种子和幼苗的脂滴大小。

The Plant Cell Pub Date : 2025-05-15 DOI: 10.1093/plcell/koaf121

Nathan M Doner,Alyssa C Clews,Nicolas Esnay,Payton S Whitehead,You Wang,Trevor B Romsdahl,Damien Seay,Philipp W Niemeyer,Martin Bonin,Yang Xu,Oliver Valerius,Gerhard H Braus,Till Ischebeck,Kent D Chapman,John M Dyer,Robert T Mullen

{"title":"LIPID DROPLET PROTEIN OF SEEDS is involved in the control of lipid droplet size in Arabidopsis seeds and seedlings.","authors":"Nathan M Doner,Alyssa C Clews,Nicolas Esnay,Payton S Whitehead,You Wang,Trevor B Romsdahl,Damien Seay,Philipp W Niemeyer,Martin Bonin,Yang Xu,Oliver Valerius,Gerhard H Braus,Till Ischebeck,Kent D Chapman,John M Dyer,Robert T Mullen","doi":"10.1093/plcell/koaf121","DOIUrl":"https://doi.org/10.1093/plcell/koaf121","url":null,"abstract":"In oilseeds, energy-rich carbon is stored as triacylglycerols in organelles called lipid droplets (LDs). While several of the major biogenetic proteins involved in LD formation have been identified, the full repertoire of LD proteins and their functional roles remains incomplete. Here, we show that the low-abundance, seed-specific LD protein LIPID DROPLET PROTEIN OF SEEDS (LDPS) contains an amphipathic α-helix and proline hairpin motif that serves as an LD-targeting signal and a separate region that binds to the LD protein OLEOSIN 1 (OLEO1). Loss of LDPS function results in smaller LDs and less seed oil in comparison to wild type, while over-expression of LDPS results in an increase in LD size and seed oil content. Loss of LDPS function also results in an inability of LDs to undergo fusion during post-germinative seedling growth. Analysis of oleo1 and ldps single and double mutant seeds and freeze-thaw treatment of seeds revealed that OLEO1 suppresses the ability of LDPS to promote larger LDs. Collectively, our results identify LDPS as an important player in LD biology that functions together with OLEO1 to determine LD size in Arabidopsis (Arabidopsis thaliana) seeds and seedlings through a process that involves LD-LD fusion.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144065804","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A magic pocket: An all-in-one CRISPR toolbox for plants. 一个神奇的口袋：一个用于植物的多功能CRISPR工具箱。

The Plant Cell Pub Date : 2025-05-14 DOI: 10.1093/plcell/koaf120

Andrea Gomez-Felipe

引用次数: 0

NUE regulons conserved model-to-crop enhance machine learning predictions of nitrogen use efficiency. NUE规则保守的模型-作物增强了氮利用效率的机器学习预测。

The Plant Cell Pub Date : 2025-05-14 DOI: 10.1093/plcell/koaf093

Ji Huang,Chia-Yi Cheng,Matthew D Brooks,Tim L Jeffers,Nathan M Doner,Hung-Jui Shih,Samantha Frangos,Manpreet Singh Katari,Gloria M Coruzzi

{"title":"NUE regulons conserved model-to-crop enhance machine learning predictions of nitrogen use efficiency.","authors":"Ji Huang,Chia-Yi Cheng,Matthew D Brooks,Tim L Jeffers,Nathan M Doner,Hung-Jui Shih,Samantha Frangos,Manpreet Singh Katari,Gloria M Coruzzi","doi":"10.1093/plcell/koaf093","DOIUrl":"https://doi.org/10.1093/plcell/koaf093","url":null,"abstract":"Systems biology aims to uncover gene regulatory networks (GRNs) for agricultural traits, but validating them in crops is challenging. We addressed this challenge by learning and validating model-to-crop GRN regulons governing nitrogen use efficiency (NUE). First, a fine-scale time-course nitrogen (N) response transcriptome analysis revealed a conserved temporal N response cascade in maize (Zea mays) and Arabidopsis (Arabidopsis thaliana). This data was used to infer time-based causal transcription factor (TF) target edges in N-regulated GRNs (N-GRNs). By validating 23 maize TFs in a cell-based TF-perturbation assay (TARGET), precision/recall analysis enabled us to prune high-confidence edges between ∼200 TFs/700 maize target genes. We next learned gene-to-NUE trait scores using XGBoost machine learning models trained on conserved N-responsive genes across maize and Arabidopsis accessions. By integrating NUE gene scores within our N-GRN, we ranked maize TFs based on a cumulative NUE regulon score. Regulons for top-ranked TFs were validated using the cell-based TARGET assay in maize (e.g. ZmMYB34/R3→24 targets) and the Arabidopsis ZmMYB34/R3 ortholog (e.g. AtDIV1→23 targets). The genes in this NUE regulon significantly enhanced the ability of XGBoost models to predict NUE traits in both maize and Arabidopsis. Thus, our pipeline for identifying NUE regulons that combines GRN inference, machine learning, and orthologous network regulons offers a strategic framework for crop trait improvement.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945487","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PROTEIN PHOSPHATASE2A B'η drives spliceosome subunit dephosphorylation to mediate alternative splicing following heat stress. 蛋白PHOSPHATASE2A B'η驱动剪接体亚基去磷酸化介导热应激后的选择性剪接。

The Plant Cell Pub Date : 2025-05-13 DOI: 10.1093/plcell/koaf117

Seung Hee Jo,Hyun Ji Park,Haemyeong Jung,Ga Seul Lee,Jeong Hee Moon,Hyun-Soon Kim,Hyo-Jun Lee,Choonkyun Jung,Hye Sun Cho

{"title":"PROTEIN PHOSPHATASE2A B'η drives spliceosome subunit dephosphorylation to mediate alternative splicing following heat stress.","authors":"Seung Hee Jo,Hyun Ji Park,Haemyeong Jung,Ga Seul Lee,Jeong Hee Moon,Hyun-Soon Kim,Hyo-Jun Lee,Choonkyun Jung,Hye Sun Cho","doi":"10.1093/plcell/koaf117","DOIUrl":"https://doi.org/10.1093/plcell/koaf117","url":null,"abstract":"Dephosphorylation of spliceosome components is an essential regulatory step for intron removal from pre-mRNA, thereby controlling gene expression. However, the specific phosphatase responsible for this dephosphorylation step has not been identified. Here, we show that Arabidopsis thaliana (Arabidopsis) PROTEIN PHOSPHATASE 2A B'η (PP2A B'η), a B subunit of PP2A, interacts with the splicing factors PRP18a, PRP16, and RH2 and facilitates their dephosphorylation by recognizing substrates through a conserved binding motif. This dephosphorylation is crucial for proper splicing of retained introns in heat stress-responsive genes, which is mediated by the PP2A interactor PRE-MRNA PROCESSING FACTOR 18a (PRP18a). Genetic inactivation of PP2A B'η abolished thermotolerance during seed germination and resulted in widespread intron retention in heat stress-responsive genes. Conversely, overexpression of PP2A B'η conferred enhanced thermotolerance, accompanied by the efficient removal of retained introns under heat stress. We demonstrate that a B regulatory subunit of PP2A plays a central role in dephosphorylating spliceosome components, regulating alternative splicing, facilitating acclimation to heat stress, and targeting specific spliceosome subunits that activate pre-mRNA splicing.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143945544","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Resistance awakens: Diversity at the DNA, RNA, and protein levels informs engineering of plant immune receptors from Arabidopsis to crops 抗性觉醒：DNA、RNA和蛋白质水平的多样性为从拟南芥到农作物的植物免疫受体工程提供了信息

The Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koaf109

Chandler A Sutherland, Danielle M Stevens, Kyungyong Seong, Wei Wei, Ksenia V Krasileva

{"title":"The Resistance awakens: Diversity at the DNA, RNA, and protein levels informs engineering of plant immune receptors from Arabidopsis to crops","authors":"Chandler A Sutherland, Danielle M Stevens, Kyungyong Seong, Wei Wei, Ksenia V Krasileva","doi":"10.1093/plcell/koaf109","DOIUrl":"https://doi.org/10.1093/plcell/koaf109","url":null,"abstract":"Plants rely on germline-encoded, innate immune receptors to sense pathogens and initiate the defense response. The exponential increase in quality and quantity of genomes, RNA-seq datasets, and protein structures has underscored the incredible biodiversity of plant immunity. Arabidopsis continues to serve as a valuable model and the theoretical foundation of our understanding of wild plant diversity of immune receptors, while expansion of study into agricultural crops has also revealed distinct evolutionary trajectories and challenges. Here, we provide the classical context for study of both intracellular nucleotide-binding, leucine-rich repeat receptors (NLRs) and surface-localized pattern recognition receptors (PRRs) at the levels of DNA sequences, transcriptional regulation, and protein structures. We then examine how recent technology has shaped our understanding of immune receptor evolution and informed our ability to efficiently engineer resistance. We summarize current literature and provide an outlook on how researchers take inspiration from natural diversity in bioengineering efforts for disease resistance from Arabidopsis and other model systems to crops.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"49 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143930851","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants. 翻译中的迷失：我们从拟南芥的特性中学到的东西，不能从拟南芥转化为其他植物。

The Plant Cell Pub Date : 2025-05-09 DOI: 10.1093/plcell/koaf036

Adrienne H K Roeder,Andrew Bent,John T Lovell,John K McKay,Armando Bravo,Karina Medina-Jimenez,Kevin W Morimoto,Siobhán M Brady,Lei Hua,Julian M Hibberd,Silin Zhong,Francesca Cardinale,Ivan Visentin,Claudio Lovisolo,Matthew A Hannah,Alex A R Webb

{"title":"Lost in translation: What we have learned from attributes that do not translate from Arabidopsis to other plants.","authors":"Adrienne H K Roeder,Andrew Bent,John T Lovell,John K McKay,Armando Bravo,Karina Medina-Jimenez,Kevin W Morimoto,Siobhán M Brady,Lei Hua,Julian M Hibberd,Silin Zhong,Francesca Cardinale,Ivan Visentin,Claudio Lovisolo,Matthew A Hannah,Alex A R Webb","doi":"10.1093/plcell/koaf036","DOIUrl":"https://doi.org/10.1093/plcell/koaf036","url":null,"abstract":"Research in Arabidopsis thaliana has a powerful influence on our understanding of gene functions and pathways. However, not everything translates from Arabidopsis to crops and other plants. Here, a group of experts consider instances where translation has been lost and why such translation is not possible or is challenging. First, despite great efforts, floral dip transformation has not succeeded in other species outside Brassicaceae. Second, due to gene duplications and losses throughout evolution, it can be complex to establish which genes are orthologs of Arabidopsis genes. Third, during evolution Arabidopsis has lost arbuscular mycorrhizal symbiosis. Fourth, other plants have evolved specialized cell types that are not present in Arabidopsis. Fifth, similarly, C4 photosynthesis cannot be studied in Arabidopsis, which is a C3 plant. Sixth, many other plant species have larger genomes, which has given rise to innovations in transcriptional regulation that are not present in Arabidopsis. Seventh, phenotypes such as acclimation to water stress can be challenging to translate due to different measurement strategies. And eighth, while the circadian oscillator is conserved, there are important nuances in the roles of circadian regulators in crop plants. A key theme emerging across these vignettes is that even when translation is lost, insights can still be gained through comparison with Arabidopsis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"129 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-09","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143992089","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0