The Plant Cell最新文献

Advancing ecological and evolutionary research in Arabidopsis: extending insights into model and non-model plants 推进拟南芥的生态和进化研究：扩展对模式和非模式植物的见解

The Plant Cell Pub Date : 2025-06-13 DOI: 10.1093/plcell/koaf151

María Verónica Arana, F Xavier Picó

{"title":"Advancing ecological and evolutionary research in Arabidopsis: extending insights into model and non-model plants","authors":"María Verónica Arana, F Xavier Picó","doi":"10.1093/plcell/koaf151","DOIUrl":"https://doi.org/10.1093/plcell/koaf151","url":null,"abstract":"Arabidopsis is regarded as the gold standard among plant systems because it has generated knowledge with translational potential across various disciplines. Nevertheless, the influence of less-explored fields within the Arabidopsis community, such as ecology and evolutionary ecology, has yet to be synthesized to emphasize their contributions to other plant disciplines. This essay summarizes current eco-evolutionary knowledge in Arabidopsis and highlights its potential to enrich the insights made by the Arabidopsis community as well as others working with other plant model and non-model systems. We underline the value of accession-based approaches but also highlight the importance for developing population-based approaches to understand how and where evolutionary change begins. Furthermore, we focus on the evolutionary value of phenotypic plasticity as necessary to comprehend the response of organisms to environmental changes. We also elaborate on conceptual and technical challenges to transcriptomic studies conducted in field conditions that evaluate gene function and gene effect on integrated phenotypes in natural environments. Overall, we believe that the development of joint eco-evolutionary and genetic research in Arabidopsis can help other plant species to develop as model systems, but the Arabidopsis community should also look at the advances of other emerging plant model systems.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"221 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycomb Repressive Complex 2 facilitates the transition from heterotrophy to photoautotrophy during seedling emergence 多梳抑制复合体2促进了苗期从异养到光自养的过渡

The Plant Cell Pub Date : 2025-06-13 DOI: 10.1093/plcell/koaf148

Naseem Samo, María Guadalupe Trejo-Arellano, Lenka Gahurová, Alexander Erban, Alina Ebert, Quentin Rivière, Jiří Kubásek, Fatemeh Aflaki, Helena Hönig Mondeková, Armin Schlereth, Annick Dubois, Mingxi Zhou, Ondřej Novák, Jiří Šantrůček, Daniel Bouyer, Francois Roudier, Joachim Kopka, Iva Mozgová

{"title":"Polycomb Repressive Complex 2 facilitates the transition from heterotrophy to photoautotrophy during seedling emergence","authors":"Naseem Samo, María Guadalupe Trejo-Arellano, Lenka Gahurová, Alexander Erban, Alina Ebert, Quentin Rivière, Jiří Kubásek, Fatemeh Aflaki, Helena Hönig Mondeková, Armin Schlereth, Annick Dubois, Mingxi Zhou, Ondřej Novák, Jiří Šantrůček, Daniel Bouyer, Francois Roudier, Joachim Kopka, Iva Mozgová","doi":"10.1093/plcell/koaf148","DOIUrl":"https://doi.org/10.1093/plcell/koaf148","url":null,"abstract":"The seed-to-seedling transition represents a key developmental and metabolic switch in plants. Catabolism of seed storage reserves fuels germination and early seedling emergence until photosynthesis is established. The seed-to-seedling developmental transition is controlled by Polycomb repressive complex 2 (PRC2). However, the coordination of PRC2 activity and its contribution to transcriptional reprogramming during seedling establishment remain unknown. By analyzing H3K27me3 re-distribution and changes in gene transcription in the shoot and root tissues of heterotrophic and photoautotrophic Arabidopsis (Arabidopsis thaliana) seedlings, we reveal two phases of PRC2-mediated gene repression. The first phase is independent of light and photosynthesis and results in the irreversible repression of the embryo maturation program, marked by heterotrophy and reserve storage molecule biosynthesis. The second phase is associated with the repression of metabolic pathways related to germination and early seedling emergence, and H3K27me3 deposition in this phase is sensitive to photosynthesis inhibition. We show that preventing the transcription of the PRC2-repressed glyoxylate cycle gene ISOCITRATE LYASE promotes the vegetative phase transition in PRC2-depleted plants. Our findings underscore a key role of PRC2-mediated transcriptional repression in the coordinated metabolic and developmental switches that occur during seedling emergence and emphasizes the close connection between metabolic and developmental identities.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pancentromere analysis of Allium species reveals diverse centromere positions in onion and gigantic centromeres in garlic 葱属植物的全着丝粒分析表明，洋葱的着丝粒位置不同，大蒜的着丝粒位置巨大

The Plant Cell Pub Date : 2025-06-10 DOI: 10.1093/plcell/koaf142

Kiyotaka Nagaki, Koichiro Ushijima, Takashi Akagi, Keisuke Tanaka, Hisato Kobayashi

{"title":"Pancentromere analysis of Allium species reveals diverse centromere positions in onion and gigantic centromeres in garlic","authors":"Kiyotaka Nagaki, Koichiro Ushijima, Takashi Akagi, Keisuke Tanaka, Hisato Kobayashi","doi":"10.1093/plcell/koaf142","DOIUrl":"https://doi.org/10.1093/plcell/koaf142","url":null,"abstract":"In eukaryotes, centromeres interact with the kinetochore for distribution of genetic information in cell division, yet their sequence and size are diverse among species. However, their position on chromosomes is considered to be conserved within a species. In this study, we analyzed the centromeres of three Allium species, namely, Welsh onion (Allium fistulosum), onion (Allium cepa), and garlic (Allium sativum) via pancentromere analysis and repetitive sequence analysis of centromeres and their neighborhoods and revealed their mobility, sequence organization, and size. Among the three species, Welsh onion and garlic had stable centromeres, but the onion centromere appeared to be polymorphic and frequently differed in position by up to 28.0 Mb among cultivars and between multiple individuals of the same cultivar. This mobility was stabilized by hybridization with Welsh onions. Furthermore, these three species have very different centromere sequence organization, including differences in the existence and maturity of centromeric satellites, and differences in centromere size, with Welsh onion having a centromere of 1.9 Mb, and garlic having a centromere of approximately 10.6 Mb, the largest of any organism with monocentric chromosomes analyzed to date. Our pancentromere analysis of these Allium species reveals the variation in sequence organization, size and position of this important chromosomal region.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144260006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core biological principles and tools stemming from basic Arabidopsis research 拟南芥基础研究的核心生物学原理和工具

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf141

Lucia C Strader, Tianyuan Chen, Xinnian Dong, David Edwards, Sridevi Sureshkumar, Sureshkumar Balasubramanian, Mauricio S Antunes, Lili Zebluim, Patarasuda Chaisupa, R Clay Wright

引用次数: 0

Regulation of glucosylceramide synthase and sphingolipid remodeling in the plant response to phosphate deficiency 植物对磷酸盐缺乏反应中葡萄糖神经酰胺合成酶和鞘脂重塑的调控

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf138

Bao Yang, Yan Peng, Guo Zhang, Ruifan Liu, Simin Hao, Yi Ren, Shaoping Lu, Xuemin Wang, Liang Guo

{"title":"Regulation of glucosylceramide synthase and sphingolipid remodeling in the plant response to phosphate deficiency","authors":"Bao Yang, Yan Peng, Guo Zhang, Ruifan Liu, Simin Hao, Yi Ren, Shaoping Lu, Xuemin Wang, Liang Guo","doi":"10.1093/plcell/koaf138","DOIUrl":"https://doi.org/10.1093/plcell/koaf138","url":null,"abstract":"Sphingolipids are important and abundant lipids in the plasma membrane, and their homeostasis plays a key role in plant growth, development, and stress responses. We previously found that non-specific phospholipase C4 (NPC4) hydrolyzes sphingophospholipids upon phosphate starvation. Here, we defined the downstream steps of sphingolipid remodeling by identifying glucosylceramide synthase (GCS) and its regulatory mechanisms in Arabidopsis thaliana. Phosphate deficiency induces the expression of GCS, and the encoded GCS enzyme mediates glucosylceramide biosynthesis. Down-regulation of GCS severely affects sphingolipid homeostasis and hinders plant growth under phosphate starvation. Accordingly, GCS over-expression promotes sphingolipid remodeling to maintain plant growth. In addition, PHOSPHATE STARVATION RESPONSE1 (PHR1), a key regulator of phosphate homeostasis, directly affects the expression of NPC4 and GCS to regulate sphingolipid remodeling during phosphate starvation. Together, these results identify the PHR1–NPC4–GCS module as a regulatory mechanism that fine-tunes sphingolipid homeostasis and reveal the importance of sphingolipid homeostasis in the plant response to phosphate deficiency.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"770 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803 连接蛋白ApcI调控Synechocystis sp. PCC 6803在红光下的光收获

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf144

Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld

{"title":"The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803","authors":"Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld","doi":"10.1093/plcell/koaf144","DOIUrl":"https://doi.org/10.1093/plcell/koaf144","url":null,"abstract":"Phycobilisomes are versatile cyanobacterial antenna complexes that harvest light energy to drive photosynthesis. They can adapt to various light conditions; for example, dismantling under high light to prevent photo-oxidation and arranging in rows under low light to increase light harvesting efficiency. Light quality also influences phycobilisome structure and function, as observed under far-red light exposure. Here, we describe a phycobilisome linker protein, ApcI (previously hypothetical protein Sll1911), expressed specifically under red light (620 nm) or upon chemically induced reduction of the plastoquinone pool. We characterized ApcI in Synechocystis sp. PCC 6803 using mutant analyses, phycobilisome binding experiments, and protein interaction studies. Deletion of apcI conferred high light tolerance on Synechocystis sp. PCC 6803 compared to the wild-type strain, leading to reduced energy transfer from phycobilisomes to the photosystems under high light. Binding experiments revealed that ApcI replaces the linker protein ApcG at the membrane-facing side of the phycobilisome core via a paralogous C-terminal motif. Additionally, the N-terminal region of ApcI interacts with photosystem II. Our findings highlight the importance of phycobilisome remodeling for adaptation to different light conditions. The characterization of ApcI provides insight into the mechanisms by which cyanobacteria optimize light harvesting in response to varying light conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips 拟南芥根尖tRNASer/tRNATyr基因簇的再激活

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf137

Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu

{"title":"Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips","authors":"Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu","doi":"10.1093/plcell/koaf137","DOIUrl":"https://doi.org/10.1093/plcell/koaf137","url":null,"abstract":"Plants maintain redundant tRNA genes (tDNA) in their nuclear genomes, but the significance, regulation, and functional roles of these genes remain poorly understood. A cluster of tandemly repeated tDNAs decoding serine and tyrosine (SYY cluster) is located on Arabidopsis (Arabidopsis thaliana) chromosome 1, intersecting constitutive heterochromatin and remaining transcriptionally silenced in most tissues. The natural conditions inducing transcription of these tDNAs remain unknown. Here, we elucidate the tissue-specific expression pattern of this cluster during seedling establishment. Our findings reveal that SYY cluster tRNAs are primarily produced in the root cap columella and adjacent root cap cells. Transcriptional reactivation of the SYY cluster occurs in these tissues despite high DNA methylation levels. Furthermore, we demonstrate that these cells accumulate high levels of a transgenic glycoprotein rich in serine, tyrosine, and proline, and that CRISPR/Cas9 deletion of the SYY cluster alters the accumulation and stability of the glycoprotein in these specific cells. Our work provides pioneering evidence of a developmental and cell-specific expression program for a plant tDNA. We offer insights into the putative role of specialized tDNAs in enhancing glycoprotein biosynthesis in protective tissues of the meristem.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato E3连接酶PIRE1的基因编辑微调活性氧的产生，以增强番茄的细菌抗病能力

The Plant Cell Pub Date : 2025-05-30 DOI: 10.1093/plcell/koaf049

Bardo Castro, Suji Baik, Megann Tran, Jie Zhu, Tianrun Li, Andrea Tang, Nathalie Aoun, Alison C Blundell, Michael Gomez, Elaine Zhang, Myeong-Je Cho, Tiffany Lowe-Power, Shahid Siddique, Brian Staskawicz, Gitta Coaker

{"title":"Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato","authors":"Bardo Castro, Suji Baik, Megann Tran, Jie Zhu, Tianrun Li, Andrea Tang, Nathalie Aoun, Alison C Blundell, Michael Gomez, Elaine Zhang, Myeong-Je Cho, Tiffany Lowe-Power, Shahid Siddique, Brian Staskawicz, Gitta Coaker","doi":"10.1093/plcell/koaf049","DOIUrl":"https://doi.org/10.1093/plcell/koaf049","url":null,"abstract":"Reactive oxygen species (ROS) accumulation is required for effective plant defense. Accumulation of the Arabidopsis (Arabidopsis thaliana) NADPH oxidase respiratory burst oxidase homolog D (RBOHD) is regulated by phosphorylation of a conserved C-terminal residue (T912) leading to ubiquitination by the RING E3 ligase Pbl13-interacting RING domain E3 ligase (PIRE). Arabidopsis PIRE knockouts exhibit enhanced ROS production and resistance to the foliar pathogen Pseudomonas syringae. Here, we identified 170 PIRE homologs, which emerged in tracheophytes and expanded in angiosperms. We investigated the role of tomato (Solanum lycopersicum) PIRE homologs in regulating ROS production, RBOH stability, and disease resistance. Mutational analyses of residues corresponding to T912 in the tomato RBOHD ortholog, SlRBOHB, affected protein accumulation and ROS production in a PIRE-dependent manner. Using genome editing, we generated mutants in 2 S. lycopersicum PIRE (SlPIRE) homologs. SlPIRE1 edited lines (Slpire1) in the tomato cultivar M82 displayed enhanced ROS production upon treatment with flg22, an immunogenic epitope of flagellin. Furthermore, Slpire1 exhibited decreased disease symptoms and bacterial accumulation when inoculated with foliar bacterial pathogens P. syringae and Xanthomonas campestris. However, Slpire1 exhibited similar levels of colonization as wild type upon inoculation with diverse soil-borne pathogens. These results indicate that PIRE regulates RBOHs in multiple plant species and is a promising target for foliar disease control. This study also highlights the pathogen-specific role of PIRE, indicating its potential for targeted manipulation to enhance foliar disease resistance without affecting root-associated pathogenic interactions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nematode effector hijacks a host RBR-type E3 ubiquitin ligase to regulate NRC4 receptor-mediated plant immunity and facilitate parasitism 一种线虫效应物劫持宿主rbr型E3泛素连接酶来调节NRC4受体介导的植物免疫并促进寄生

The Plant Cell Pub Date : 2025-05-27 DOI: 10.1093/plcell/koaf125

Xin Qin, Jiarong Yu, Wenjun Hu, Chen Chen, Cong Chen, Yuqi Shi, Yuwen Jiang, Shuai Zeng, Jun Hu, Ruiyan Wang, Xiaohua Yang, Xuan Wang

{"title":"A nematode effector hijacks a host RBR-type E3 ubiquitin ligase to regulate NRC4 receptor-mediated plant immunity and facilitate parasitism","authors":"Xin Qin, Jiarong Yu, Wenjun Hu, Chen Chen, Cong Chen, Yuqi Shi, Yuwen Jiang, Shuai Zeng, Jun Hu, Ruiyan Wang, Xiaohua Yang, Xuan Wang","doi":"10.1093/plcell/koaf125","DOIUrl":"https://doi.org/10.1093/plcell/koaf125","url":null,"abstract":"The root-knot nematode Meloidogyne incognita is an obligate biotrophic pathogen that causes extensive losses to agriculture worldwide. Effectors secreted by the parasite play an essential role during nematode infection through suppressing plant innate immunity. Here, we identify and characterize a M. incognita effector designated as MiV86, which is secreted into plant cells and positively regulates nematode parasitism. We show that MiV86 interacts with RING finger protein 217 (NbRNF217), an RBR-type E3 ubiquitin ligase of Nicotiana benthamiana, which negatively regulates plant immunity in an enzymatic activity-dependent manner. Moreover, we demonstrate that NbRNF217 targets and ubiquitinates the helper nucleotide-binding leucine-rich repeat receptor protein NRC4, resulting in its relocation and degradation through the 26S proteasome and endosomal/vacuolar pathways. NbRNF217 regulates its homeostasis through self-catalyzed ubiquitination or external ubiquitination modifications, and we show that MiV86 inhibits the ubiquitination of NbRNF217 in planta without affecting its activity, thereby promoting the degradation of NRC4, which also contributes to the resistance of N. benthamiana against M. incognita. Our findings reveal a mechanism by which a nematode effector hijacks an E3 ubiquitin ligase to attenuate NRC4-mediated plant immunity, facilitating nematode parasitism.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The histone acetyltransferase GCN5 regulates floral meristem activity and flower development in Arabidopsis 组蛋白乙酰转移酶GCN5调控拟南芥花分生组织活性和花发育

The Plant Cell Pub Date : 2025-05-25 DOI: 10.1093/plcell/koaf135

Amangul Hawar, Wei Chen, Tao Zhu, Xin Wang, Jiaxin Liu, Shiqi Xiong, Toshiro Ito, Dijun Chen, Bo Sun

{"title":"The histone acetyltransferase GCN5 regulates floral meristem activity and flower development in Arabidopsis","authors":"Amangul Hawar, Wei Chen, Tao Zhu, Xin Wang, Jiaxin Liu, Shiqi Xiong, Toshiro Ito, Dijun Chen, Bo Sun","doi":"10.1093/plcell/koaf135","DOIUrl":"https://doi.org/10.1093/plcell/koaf135","url":null,"abstract":"The histone acetyltransferase GENERAL CONTROL NON DEREPRESSIBLE5 (GCN5) participates in various developmental processes in Arabidopsis (Arabidopsis thaliana). Notably, GCN5 ensures proper flower development, but the underlying mechanism remains unknown. Here, we show that during early flower development, GCN5 catalyzes histone acetylation at WUSCHEL (WUS) and CLAVATA3 (CLV3) chromatin, activating their expression. WUS and CLV3 are required for floral meristem (FM) maintenance. Furthermore, we demonstrate that the GCN5–ALTERATION/DEFICIENCY IN ACTIVATION 2 (ADA2) histone acetyltransferase module interacts with the Switch/Sucrose non-fermentable ATPase SPLAYED (SYD) to form a GCN5–ADA2b–SYD ternary complex. The cytokinin responsive type-B Arabidopsis response regulators (ARRs) recruit this ternary complex for WUS activation. During floral organogenesis, PERIANTHIA recruits the GCN5–ADA2b module for AGAMOUS activation, which promotes FM determinacy. GCN5 also activates KNUCKLES, which ensures the timely termination of FM activity. Moreover, GCN5 modulates the expression pattern of the B-class gene APETALA3 and promotes the expression of SUPERMAN and CRABS CLAW, which are required for FM determinacy, thereby safeguarding meristem determinacy and correct floral organ formation. Thus, our study demonstrates the indispensable role of GCN5 in establishing a permissive chromatin environment to regulate the key genes required for precise flower development.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0