The Plant Cell最新文献

Heat stress triggers enhanced nuclear localization of HYL1 to regulate miRNA biogenesis and thermotolerance in plants 热胁迫引发HYL1核定位增强，调控植物miRNA生物发生和耐热性

The Plant Cell Pub Date : 2025-04-23 DOI: 10.1093/plcell/koaf092

Yiming Cao, Jiaxin Zhang, Zhong Zhao, Guiliang Tang, Jun Yan

{"title":"Heat stress triggers enhanced nuclear localization of HYL1 to regulate miRNA biogenesis and thermotolerance in plants","authors":"Yiming Cao, Jiaxin Zhang, Zhong Zhao, Guiliang Tang, Jun Yan","doi":"10.1093/plcell/koaf092","DOIUrl":"https://doi.org/10.1093/plcell/koaf092","url":null,"abstract":"Plants have evolved a complex regulatory network to cope with heat stress (HS), which includes microRNAs (miRNAs). However, the roles of the entire miRNA biogenesis machinery in HS responses remain unclear. Here, we show that HS induces the majority of miRNAs primarily through the enhanced nuclear localization of HYPONASTIC LEAVES 1 (HYL1), rather than by upregulating MIR gene transcription in Arabidopsis (Arabidopsis thaliana). Disruption of miRNA biogenesis increases plant susceptibility to HS. We also demonstrate that HYL1 phosphorylation modulates its nuclear localization during HS, which is critical for miRNA induction and thermotolerance. MAP KINASE3 (MPK3) phosphorylates and stabilizes the phosphatase C-TERMINAL DOMAIN PHOSPHATASE-LIKE 1 (CPL1), while CPL1 inhibits MPK3 activity, creating a feedback loop that regulates HYL1 phosphorylation. Disruption of MPK3 function results in increased nuclear HYL1 levels and miRNA production, conferring enhanced HS tolerance to mpk3 mutants. These findings reveal a mechanism by which plants enhance miRNA biogenesis during HS, offering insights into the regulatory networks that govern plant thermotolerance.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"37 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143866495","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factors DOF4.6 and XND1 jointly regulate root hydraulics and drought responses in Arabidopsis 转录因子DOF4.6和XND1共同调控拟南芥根系水力学和干旱响应

The Plant Cell Pub Date : 2025-04-22 DOI: 10.1093/plcell/koaf083

Bingli Ding, Mengyu Liang, Yafei Shi, Runling Zhang, Jingjing Wang, Yupu Huang, Dawei Yan, Xin Hou, Christophe Maurel, Ning Tang

{"title":"The transcription factors DOF4.6 and XND1 jointly regulate root hydraulics and drought responses in Arabidopsis","authors":"Bingli Ding, Mengyu Liang, Yafei Shi, Runling Zhang, Jingjing Wang, Yupu Huang, Dawei Yan, Xin Hou, Christophe Maurel, Ning Tang","doi":"10.1093/plcell/koaf083","DOIUrl":"https://doi.org/10.1093/plcell/koaf083","url":null,"abstract":"Water uptake by roots is essential for plant growth and stress acclimation. We previously showed that the XYLEM NAC DOMAIN 1 (XND1) transcription factor negatively regulates root hydraulic conductivity (Lpr) in Arabidopsis (Arabidopsis thaliana). Here, we show that XND1 physically interacts with the transcription factor DNA-binding with One Finger 4.6 (DOF4.6). Analyses of loss-of-function mutants and overexpression lines revealed that, similar to XND1, DOF4.6 negatively regulates Lpr. DOF4.6 and XND1 jointly modulate downstream gene expression, inhibiting root xylem formation. Notably, DOF4.6 facilitates XND1 binding to the promoter of XYLEM CYSTEINE PROTEASE 1, the product of which controls programmed cell death during xylem development. DOF4.6 also independently binds to the promoters of the aquaporins PIP2;5 and PIP2;6, thereby suppressing their expression and exerting potential direct regulatory effects on membrane water transport. Importantly, the dof4.6 loss-of-function alleles showed significantly enhanced resistance to drought stress. Collectively, our findings demonstrate that DOF4.6 plays a crucial role in root hydraulics and drought stress responses, partially in concert with XND1 and through combined effects on xylem formation and aquaporin functions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The protein kinases KIPK and KIPK-LIKE1 suppress overbending during negative hypocotyl gravitropic growth in Arabidopsis 蛋白激酶KIPK和KIPK- like1抑制拟南芥负下胚轴向地生长过程中的过弯

The Plant Cell Pub Date : 2025-04-22 DOI: 10.1093/plcell/koaf056

Yao Xiao, Melina Zourelidou, Alkistis E Lanassa Bassukas, Benjamin Weller, Dorina P Janacek, Jan Šimura, Karin Ljung, Ulrich Z Hammes, Jia Li, Claus Schwechheimer

{"title":"The protein kinases KIPK and KIPK-LIKE1 suppress overbending during negative hypocotyl gravitropic growth in Arabidopsis","authors":"Yao Xiao, Melina Zourelidou, Alkistis E Lanassa Bassukas, Benjamin Weller, Dorina P Janacek, Jan Šimura, Karin Ljung, Ulrich Z Hammes, Jia Li, Claus Schwechheimer","doi":"10.1093/plcell/koaf056","DOIUrl":"https://doi.org/10.1093/plcell/koaf056","url":null,"abstract":"Plants use environmental cues to orient organ and plant growth, such as the direction of gravity or the direction, quantity, and quality of light. During the germination of Arabidopsis thaliana seeds in soil, negative gravitropism responses direct hypocotyl elongation such that the seedling can reach the light for photosynthesis and autotrophic growth. Similarly, hypocotyl elongation in the soil also requires mechanisms to efficiently grow around obstacles such as soil particles. Here, we identify KIPK (KINESIN-LIKE CALMODULIN-BINDING PROTEIN-INTERACTING PROTEIN KINASE) and the paralogous KIPKL1 (KIPK-LIKE1) as genetically redundant regulators of gravitropic hypocotyl bending. Moreover, we demonstrate that the homologous KIPKL2 (KIPK-LIKE2), which shows strong sequence similarity, must be functionally distinct. KIPK and KIPKL1 are polarly localized plasma membrane-associated proteins that can activate PIN-FORMED auxin transporters. KIPK and KIPKL1 are required to efficiently align hypocotyl growth with the gravity vector when seedling hypocotyls are grown on media plates or in soil, where contact with soil particles and obstacle avoidance impede direct negative gravitropic growth. Therefore, the polar KIPK and KIPKL1 kinases have different biological functions from the related AGC1 family kinases D6PK (D6 PROTEIN KINASE) or PAX (PROTEIN KINASE ASSOCIATED WITH BRX).","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143863070","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light 太暗，太亮，和刚刚好：衣藻在限制和过量光线下的昼夜程序的系统分析

The Plant Cell Pub Date : 2025-04-19 DOI: 10.1093/plcell/koaf086

Sunnyjoy Dupuis, Valle Ojeda, Sean D Gallaher, Samuel O Purvine, Anne G Glaesener, Raquel Ponce, Carrie D Nicora, Kent Bloodsworth, Mary S Lipton, Krishna K Niyogi, Masakazu Iwai, Sabeeha S Merchant

{"title":"Too dim, too bright, and just right: Systems analysis of the Chlamydomonas diurnal program under limiting and excess light","authors":"Sunnyjoy Dupuis, Valle Ojeda, Sean D Gallaher, Samuel O Purvine, Anne G Glaesener, Raquel Ponce, Carrie D Nicora, Kent Bloodsworth, Mary S Lipton, Krishna K Niyogi, Masakazu Iwai, Sabeeha S Merchant","doi":"10.1093/plcell/koaf086","DOIUrl":"https://doi.org/10.1093/plcell/koaf086","url":null,"abstract":"Photosynthetic organisms coordinate their metabolism and growth with diurnal light, which can range in intensity from limiting to excessive. Little is known about how light intensity impacts the diurnal program in Chlamydomonas reinhardtii, nor how diurnal rhythms in gene expression and metabolism shape photoprotective responses at different times of day. To address these questions, we performed a systems analysis of synchronized Chlamydomonas populations acclimated to low, moderate, and high diurnal light. Transcriptomic and proteomic data revealed that the Chlamydomonas rhythmic gene expression program is resilient to limiting and excess light: genome-wide, waves of transcripts and proteins peak at the same times in populations acclimated to stressful light intensities as in populations acclimated to moderate light. Yet, diurnal photoacclimation gives rise to hundreds of gene expression changes, even at night. Time-course measurements of photosynthetic efficiency and pigments responsive to excess light showed that high-light-acclimated cells partially overcome photodamage in the latter half of the day prior to cell division. Although gene expression and photodamage are dynamic over the diurnal cycle, Chlamydomonas populations acclimated to low and high diurnal light maintain altered photosystem abundance, thylakoid architecture, and non-photochemical quenching capacity through the night phase. This suggests that cells remember or anticipate the light intensities that they have typically encountered during the day. The integrated data constitute an excellent resource for understanding photoacclimation in eukaryotes under environmentally relevant conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"61 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143849759","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Integrating Arabidopsis and crop species gene discovery for crop improvement 整合拟南芥与作物物种基因发现用于作物改良

The Plant Cell Pub Date : 2025-04-19 DOI: 10.1093/plcell/koaf087

Michael W Bevan, Maxim Messerer, Heidrun Gundlach, Nadia Kamal, Anthony Hall, Manuel Spannagl, Klaus F X Mayer

{"title":"Integrating Arabidopsis and crop species gene discovery for crop improvement","authors":"Michael W Bevan, Maxim Messerer, Heidrun Gundlach, Nadia Kamal, Anthony Hall, Manuel Spannagl, Klaus F X Mayer","doi":"10.1093/plcell/koaf087","DOIUrl":"https://doi.org/10.1093/plcell/koaf087","url":null,"abstract":"Summary Genome sequence assemblies form a durable and precise framework that supports nearly all areas of biological research, including evolutionary biology, taxonomy and conservation, pathogen population diversity, crop domestication and biochemistry. In the early days of plant genomics, resources were limited to a handful of tractable genomes, leading to a tension between focus on discovering mechanisms in experimental species such as Arabidopsis thaliana (Arabidopsis) and on trait analyses in crop species. This tension arose from difficulties in translating knowledge of gene function across the large evolutionary distances between Arabidopsis and diverse crop species without comparative genome support. For some time, these clashing interests influenced funding priorities in plant science that limited both the acquisition of knowledge of mechanisms in Arabidopsis and the timely development of the capacity of crop science to incorporate knowledge of genes and their mechanisms. In this review we show how advances in genomics analysis technologies are revealing a high degree of conservation of molecular mechanisms between evolutionarily distant plant species. This progress is bridging the model-species-to-crop barrier, resulting in ever-increasing unification of plant science that is now accelerating progress in understanding mechanisms underlying diverse traits in crops and improving their performance. We lay out some examples of important priorities and outcomes arising from these new opportunities.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"65 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143851004","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Shedding light on shade-avoidance: SIPIF8a plays a pivotal role in the tomato shade response. 揭示遮荫回避：SIPIF8a在番茄遮荫反应中起关键作用。

The Plant Cell Pub Date : 2025-04-18 DOI: 10.1093/plcell/koaf091

Róisín Fattorini

引用次数: 0

ANAPHASE-PROMOTING COMPLEX/CYCLOSOME coactivators maintain AURORA1 kinase homeostasis during meiotic chromosome segregation 在减数分裂染色体分离过程中，后期促进复合体/环小体共激活因子维持AURORA1激酶的稳态

The Plant Cell Pub Date : 2025-04-17 DOI: 10.1093/plcell/koaf089

Jing Xu, Lian Zhou, Kaixin Chen, Runsen Huang, Baixiao Niu, Juanying Ye, Hong Ma, Gregory P Copenhaver, Yingxiang Wang

{"title":"ANAPHASE-PROMOTING COMPLEX/CYCLOSOME coactivators maintain AURORA1 kinase homeostasis during meiotic chromosome segregation","authors":"Jing Xu, Lian Zhou, Kaixin Chen, Runsen Huang, Baixiao Niu, Juanying Ye, Hong Ma, Gregory P Copenhaver, Yingxiang Wang","doi":"10.1093/plcell/koaf089","DOIUrl":"https://doi.org/10.1093/plcell/koaf089","url":null,"abstract":"Faithful chromosome segregation is essential for both mitotic and meiotic cell division. The Anaphase Promoting Complex/Cyclosome (APC/C) and its coactivators are required for meiotic chromosome segregation, but their potential targets and regulatory mechanisms remain unclear in plants. Here, we performed a ubiquitinome analysis and show that Arabidopsis thaliana Aurora 1 (AUR1) is over-ubiquitinated at lysine 102 in the coactivator Cell Division Cycle 20.1 (cdc20.1) mutants and that AUR1 overexpression can partially rescue the cdc20.1 meiotic defect. We also demonstrate that APC/C ubiquitinates AUR1, leading to its degradation through the 26S proteasome pathway. Moreover, the APC/C subunit and coactivators Cell Cycle Switch 52 A2/B (CCS52A2/B) and CDC20.1 interact with AUR1 both in vitro and in vivo. Intriguingly, CCS52A2/B promotes AUR1 ubiquitination and degradation, while CDC20.1 prevents AUR1 degradation. Consistent with this finding, AUR1 levels are lower in cdc20.1 and higher in ccs52 mutants relative to Col-0, and mutation of CCS52A2/B causes defects in meiotic spindle assembly and homologous chromosome segregation. Genetic analyses demonstrate that Arabidopsis Anaphase-Promoting Complex/Cyclosome subunit 8 (APC8), CDC20.1, CCS52 and AUR1 act in the same pathway to control meiotic spindle assembly and homologous chromosome segregation. Thus, this work provides mechanistic insight into the role of APC/C coactivators in regulating AUR1 homeostasis during meiosis in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847176","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor 转录因子MYB30通过维持FIT转录因子的稳定性来促进铁稳态

The Plant Cell Pub Date : 2025-04-17 DOI: 10.1093/plcell/koaf090

Hongyun Zhao, Juntao Jiang, Mengai Shen, Yiyi Zhang, Yamei Zhang, Huilin Liu, Huapeng Zhou, Yuan Zheng

{"title":"The transcription factor MYB30 promotes iron homeostasis by maintaining the stability of the FIT transcription factor","authors":"Hongyun Zhao, Juntao Jiang, Mengai Shen, Yiyi Zhang, Yamei Zhang, Huilin Liu, Huapeng Zhou, Yuan Zheng","doi":"10.1093/plcell/koaf090","DOIUrl":"https://doi.org/10.1093/plcell/koaf090","url":null,"abstract":"Iron (Fe) is a vital nutrient for the growth and development of plants. In Arabidopsis (Arabidopsis thaliana), the bHLH transcription factor FER-LIKE IRON DEFICIENCY INDUCED TRANSCRIPTION FACTOR (FIT) plays a pivotal role in regulating the response to Fe deficiency. Our study reveals that the R2R3-MYB transcription factor MYB30 is a positive regulator of the Fe-deficiency response by regulating FIT stability. Plants with loss-of-function mutations in MYB30 exhibit pronounced Fe-deficiency symptoms and diminished Fe uptake, while overexpression of MYB30 leads to the opposite effects. We have discovered that MYB30 interacts with BRUTUS LIKE1 (BTSL1) and BTSL2, two partially redundant E3 ubiquitin ligases that negatively regulate the Fe-deficiency response. MYB30 binds to the C-terminal region of BTSL1 through its MYB DNA-binding domain, thereby safeguarding FIT from BTSL1-mediated ubiquitination and degradation, resulting in FIT accumulation for Fe deficiency response. In summary, our research uncovers the role of the transcription factor MYB30 as a regulator of FIT stability, which in turn modulates Fe homeostasis in plants in response to Fe deficiency.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-17","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143847062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Storming the barricades of rhamnogalacturonan-II synthesis and function 冲破鼠李糖半乳糖醛酸- ii合成和功能的障碍

The Plant Cell Pub Date : 2025-04-16 DOI: 10.1093/plcell/koaf088

Quentin Hays, Patrice Lerouge, Marc Ropitaux, Charles T Anderson, Arnaud Lehner

引用次数: 0

SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress SIZ1 SUMOylates 并稳定 WRI1，在高温胁迫下保护种子充实和脂肪酸的生物合成

The Plant Cell Pub Date : 2025-04-16 DOI: 10.1093/plcell/koaf085

Ruihua Huang, Mengrui Wen, Bojin Feng, Pingzhi Wu, Xiaoqing Zhong, Yifeng Yang, Minghui Liu, Hongqing Li, Chengwei Yang, Changlian Peng, Shengchun Zhang

{"title":"SIZ1 SUMOylates and stabilizes WRI1 to safeguard seed filling and fatty acid biosynthesis under high-temperature stress","authors":"Ruihua Huang, Mengrui Wen, Bojin Feng, Pingzhi Wu, Xiaoqing Zhong, Yifeng Yang, Minghui Liu, Hongqing Li, Chengwei Yang, Changlian Peng, Shengchun Zhang","doi":"10.1093/plcell/koaf085","DOIUrl":"https://doi.org/10.1093/plcell/koaf085","url":null,"abstract":"High-temperature stress hinders seed filling, reducing seed quality and crop yield. However, the molecular mechanisms underlying this process remain unclear. Here, we identify SAP AND MIZ1 DOMAIN-CONTAINING LIGASE1 (SIZ1) as a key regulator of seed filling under prolonged high temperatures in Arabidopsis (Arabidopsis thaliana). SIZ1 and WRINKLED1 (WRI1) are co-expressed during seed filling, and overexpressing either gene enhances seed filling and promotes fatty acid biosynthesis under high-temperature stress. Genetic and biochemical analyses revealed that SIZ1 stabilizes WRI1 by promoting its SUMOylation at Lys-257 and Lys-266, thereby inhibiting its interaction with the CULLIN3-based ubiquitin E3 ligase adaptor protein BTB/POZMATH (BPM) and preventing its ubiquitination and degradation. Mutating these SUMOylation sites accelerates WRI1 degradation, impairing its function in seed filling under high-temperature stress. Furthermore, high-temperature stress induces SIZ1 expression and reduces WRI1 levels, suggesting that SIZ1-mediated SUMOylation counteracts high-temperature stress-induced WRI1 instability. These findings establish SIZ1 as a crucial factor maintaining WRI1 stability and seed filling under high-temperature stress, providing valuable genetic resources and a theoretical foundation for addressing prolonged high-temperature stress in agricultural production.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143841623","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0