The Plant Cell最新文献

Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis 定量蛋白质组学揭示拟南芥中广泛的赖氨酸泛素化和转录因子稳定状态

The Plant Cell Pub Date : 2024-11-21 DOI: 10.1093/plcell/koae310

Gaoyuan Song, Christian Montes, Damilola Olatunji, Shikha Malik, Chonghui Ji, Natalie M Clark, Yunting Pu, Dior R Kelley, Justin W Walley

{"title":"Quantitative proteomics reveals extensive lysine ubiquitination and transcription factor stability states in Arabidopsis","authors":"Gaoyuan Song, Christian Montes, Damilola Olatunji, Shikha Malik, Chonghui Ji, Natalie M Clark, Yunting Pu, Dior R Kelley, Justin W Walley","doi":"10.1093/plcell/koae310","DOIUrl":"https://doi.org/10.1093/plcell/koae310","url":null,"abstract":"Protein activity, abundance, and stability can be regulated by posttranslational modification including ubiquitination. Ubiquitination is conserved among eukaryotes and plays a central role in modulating cellular function, yet we lack comprehensive catalogs of proteins that are modified by ubiquitin in plants. In this study, we describe an antibody-based approach to enrich ubiquitinated peptides coupled with isobaric labeling to enable quantification of up to 18-multiplexed samples. This approach identified 17,940 ubiquitinated lysine sites arising from 6,453 proteins from Arabidopsis (Arabidopsis thaliana) primary roots, seedlings, and rosette leaves. Gene ontology analysis indicated that ubiquitinated proteins are associated with numerous biological processes including hormone signaling, plant defense, protein homeostasis, and metabolism. We determined ubiquitinated lysine residues that directly regulate the stability of three transcription factors, CRYPTOCHROME-INTERACTING BASIC-HELIX-LOOP-HELIX 1 (CIB1), CIB1 LIKE PROTEIN 2 (CIL2), and SENSITIVE TO PROTON RHIZOTOXICITY1 (STOP1) using in vivo degradation assays. Furthermore, codon mutation of CIB1 to create a K166R conversion to prevent ubiquitination, via CRISPR/Cas9-derived adenosine base editing, led to an early flowering phenotype and increased expression of FLOWERING LOCUS T (FT). These comprehensive site-level ubiquitinome profiles provide a wealth of data for future functional studies related to modulation of biological processes mediated by this posttranslational modification in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"71 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-21","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142684316","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OXI1 kinase regulates plant immunity by linking microbial pattern-induced ROS burst to MAPK activation OXI1 激酶通过将微生物模式诱导的 ROS 暴发与 MAPK 激活联系起来来调节植物免疫力

The Plant Cell Pub Date : 2024-11-20 DOI: 10.1093/plcell/koae311

Miaomiao Ma, Pan Wang, Rubin Chen, Mei Bai, Zhuoyuan He, Dan Xiao, Guangyuan Xu, Hong Wu, Jian-Min Zhou, Daolong Dou, Guozhi Bi, Xiangxiu Liang

{"title":"The OXI1 kinase regulates plant immunity by linking microbial pattern-induced ROS burst to MAPK activation","authors":"Miaomiao Ma, Pan Wang, Rubin Chen, Mei Bai, Zhuoyuan He, Dan Xiao, Guangyuan Xu, Hong Wu, Jian-Min Zhou, Daolong Dou, Guozhi Bi, Xiangxiu Liang","doi":"10.1093/plcell/koae311","DOIUrl":"https://doi.org/10.1093/plcell/koae311","url":null,"abstract":"Plant cell-surface-localized pattern recognition receptors (PRRs) recognize microbial patterns and activate pattern-triggered immunity (PTI). Typical PTI responses include reactive oxygen species (ROS) burst controlled by the NADPH oxidase RESPIRATORY BURST OXIDASE HOMOLOGUE D (RbohD) and activation of the mitogen-activated protein kinase (MAPK) cascade composed of MAPKKK3/5-MKK4/5-MPK3/6. However, the mechanisms through which PRRs regulate and coordinate these immune responses are not fully understood. Here, we showed that Arabidopsis thaliana OXIDATIVE SIGNAL-INDUCIBLE1 (OXI1), a kinase known to be activated by ROS, is involved in the LYK5-CERK1 receptor complex, which recognizes fungal cell wall-derived chitin. The oxi1 mutant exhibits enhanced susceptibility to various pathogens and reduced chitin-induced MAPK activation and ROS burst. We showed that chitin induces the phosphorylation of OXI1 in an RbohD-dependent manner. H2O2 and chitin treatment causes the oxidation of OXI1 at Cys104 and Cys205, which is essential for the kinase activity of OXI1. These oxidation sites are required for chitin-induced MAPK activation and disease resistance. Activated OXI1 directly phosphorylates MAPKKK5 to regulate MAPK activation. Additionally, OXI1 phosphorylates RbohD, suggesting that it may activate RbohD to promote ROS burst to further enhance the long-term MAPK activation. Together, our findings reveal a pathway linking PRR-mediated ROS production to MAPK activation through OXI1.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"23 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142678865","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The FERONIA–RESPONSIVE TO DESSICATION 26 module regulates vascular immunity to Ralstonia solanacearum FERONIA-RESPONSIVE TO DESSICATION 26 模块调节血管对茄属拉氏菌的免疫力

The Plant Cell Pub Date : 2024-11-13 DOI: 10.1093/plcell/koae302

Bingqian Wang, Cailin Luo, Xiaoxu Li, Alvaro Jimenez, Jun Cai, Jia Chen, Changsheng Li, Chunhui Zhang, Lijun Ou, Wenxuan Pu, Yu Peng, Zhenchen Zhang, Yong Cai, Marc Valls, Dousheng Wu, Feng Yu

{"title":"The FERONIA–RESPONSIVE TO DESSICATION 26 module regulates vascular immunity to Ralstonia solanacearum","authors":"Bingqian Wang, Cailin Luo, Xiaoxu Li, Alvaro Jimenez, Jun Cai, Jia Chen, Changsheng Li, Chunhui Zhang, Lijun Ou, Wenxuan Pu, Yu Peng, Zhenchen Zhang, Yong Cai, Marc Valls, Dousheng Wu, Feng Yu","doi":"10.1093/plcell/koae302","DOIUrl":"https://doi.org/10.1093/plcell/koae302","url":null,"abstract":"Some pathogens colonize plant leaves, but others invade the roots, including the vasculature, causing severe disease symptoms. Plant innate immunity has been extensively studied in leaf pathosystems; however, the precise regulation of immunity against vascular pathogens remains largely unexplored. We previously demonstrated that loss of function of the receptor kinase FERONIA (FER) increases plant resistance to the typical vascular bacterial pathogen Ralstonia solanacearum. Here, we show that upon infection with R. solanacearum, root xylem cell walls in Arabidopsis thaliana become highly lignified. FER is specifically upregulated in the root xylem in response to R. solanacearum infection, and inhibits lignin biosynthesis and resistance to this pathogen. We determined that FER interacts with and phosphorylates the transcription factor RESPONSIVE TO DESICCATION 26 (RD26), leading to its degradation. Overexpression and knockout of RD26 demonstrated that it positively regulates plant resistance to R. solanacearum by directly activating the expression of lignin-related genes. Tissue-specific expression of RD26 in the root xylem confirmed its role in vascular immunity. We confirmed that the FER–RD26 module regulates lignin biosynthesis and resistance against R. solanacearum in tomato (Solanum lycopersicum). Taken together, our findings unveil that the FER–RD26 cascade governs plant immunity against R. solanacearum in vascular tissues by regulating lignin deposition. This cascade may represent a key defense mechanism against vascular pathogens in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-11-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142610076","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria 磷酸酮醇酶和 KDPG 醛缩酶代谢调节蓝藻的光合碳产量

The Plant Cell Pub Date : 2024-10-29 DOI: 10.1093/plcell/koae291

Ningdong Xie, Chetna Sharma, Katherine Rusche, Xin Wang

{"title":"Phosphoketolase and KDPG aldolase metabolisms modulate photosynthetic carbon yield in cyanobacteria","authors":"Ningdong Xie, Chetna Sharma, Katherine Rusche, Xin Wang","doi":"10.1093/plcell/koae291","DOIUrl":"https://doi.org/10.1093/plcell/koae291","url":null,"abstract":"Cyanobacteria contribute to roughly a quarter of global net carbon fixation. During diel light/dark growth, dark respiration substantially lowers the overall photosynthetic carbon yield in cyanobacteria and other phototrophs. How respiratory pathways participate in carbon resource allocation at night to optimize dark survival and support daytime photosynthesis remains unclear. Here, using the cyanobacterium Synechococcus elongatus PCC 7942, we show that phosphoketolase integrates into a respiratory network in the dark to best allocate carbon resources for amino acid biosynthesis and to prepare for photosynthesis reinitiation upon photoinduction. Moreover, we show that the respiratory Entner-Doudoroff (ED) pathway in S. elongatus is incomplete, with its key enzyme 2-keto-3-deoxy-6-phosphogluconate (KDPG) aldolase exhibiting alternative oxaloacetate decarboxylation activity that modulates daytime photosynthesis. This activity allows for the bypassing of the tricarboxylic acid (TCA) cycle when ATP and NADPH consumption for biosynthesis is excessive and imbalanced relative to their production by the light reactions, thereby preventing relative NADPH accumulation and ensuring optimal photosynthetic carbon yield. Optimizing these metabolic processes offers opportunities to enhance photosynthetic carbon yield in cyanobacteria and other photosynthetic organisms under diel light/dark cycles.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"16 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142541279","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis 激酶 ATR 在拟南芥基因组尺度上控制减数分裂交叉分布

The Plant Cell Pub Date : 2024-10-29 DOI: 10.1093/plcell/koae292

Longfei Zhu, Julia Dluzewska, Nadia Fernández-Jiménez, Rajeev Ranjan, Alexandre Pelé, Wojciech Dziegielewski, Maja Szymanska-Lejman, Karolina Hus, Julia Górna, Mónica Pradillo, Piotr A Ziolkowski

{"title":"The kinase ATR controls meiotic crossover distribution at the genome scale in Arabidopsis","authors":"Longfei Zhu, Julia Dluzewska, Nadia Fernández-Jiménez, Rajeev Ranjan, Alexandre Pelé, Wojciech Dziegielewski, Maja Szymanska-Lejman, Karolina Hus, Julia Górna, Mónica Pradillo, Piotr A Ziolkowski","doi":"10.1093/plcell/koae292","DOIUrl":"https://doi.org/10.1093/plcell/koae292","url":null,"abstract":"Meiotic crossover, i.e., the reciprocal exchange of chromosome fragments during meiosis, is a key driver of genetic diversity. Crossover is initiated by the formation of programmed DNA double-strand breaks (DSBs). While the role of ATAXIA-TELANGIECTASIA AND RAD3-RELATED (ATR) kinase in DNA damage signaling is well-known, its impact on crossover formation remains understudied. Here, using measurements of recombination at chromosomal intervals and genome-wide crossover mapping, we showed that ATR inactivation in Arabidopsis (Arabidopsis thaliana) leads to dramatic crossover redistribution, with an increase in crossover frequency in chromosome arms and a decrease in pericentromeres. These global changes in crossover placement were not caused by alterations in DSB numbers, which we demonstrated by analyzing phosphorylated H2A.X foci in zygonema. Using the seed-typing technique, we found that hotspot usage remains mainly unchanged in atr mutants compared to wild-type individuals. Moreover, atr showed no change in the number of crossovers caused by two independent pathways, which implies no effect on crossover pathway choice. Analyses of genetic interaction indicate that while the effects of atr are independent of MMS AND UV SENSITIVE81 (MUS81), ZIPPER1 (ZYP1), FANCONI ANEMIA COMPLEMENTATION GROUP M (FANCM) and D2 (FANCD2), the underlying mechanism may be similar between ATR and FANCD2. This study extends our understanding of ATR’s role in meiosis, uncovering functions in regulating crossover distribution.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"86 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142541280","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ribosome binding of phasiRNA precursors accelerates the 24-nt phasiRNA burst in meiotic maize anthers 核糖体与 phasiRNA 前体的结合加速了减数分裂期玉米花药中 24-nt phasiRNA 的迸发

The Plant Cell Pub Date : 2024-10-23 DOI: 10.1093/plcell/koae289

Yingjia Han, Siqi Jiang, Xiaomei Dong, Xing Dai, Shunxi Wang, Ying Zheng, Ge Yan, Shengben Li, Liuji Wu, Virginia Walbot, Blake C Meyers, Mei Zhang

{"title":"Ribosome binding of phasiRNA precursors accelerates the 24-nt phasiRNA burst in meiotic maize anthers","authors":"Yingjia Han, Siqi Jiang, Xiaomei Dong, Xing Dai, Shunxi Wang, Ying Zheng, Ge Yan, Shengben Li, Liuji Wu, Virginia Walbot, Blake C Meyers, Mei Zhang","doi":"10.1093/plcell/koae289","DOIUrl":"https://doi.org/10.1093/plcell/koae289","url":null,"abstract":"Reproductive phasiRNAs (phased, small interfering RNAs), produced from numerous PHAS loci, are essential for plant anther development. PHAS transcripts are enriched on endoplasmic reticulum-bound ribosomes in maize (Zea mays), but the impact of ribosome binding on phasiRNA biogenesis remains elusive. Through ribosome profiling of maize anthers at 10 developmental stages, we demonstrated that 24-PHAS transcripts are bound by ribosomes, with patterns corresponding to the timing and abundance of 24-PHAS transcripts. Ribosome binding to 24-PHAS transcripts is conserved among different maize inbred lines, with ribosomes enriched upstream of miR2275 target sites. We detected short open reading frames (sORFs) in the ribosome-binding regions of some 24-PHAS transcripts and observed a 3-nt periodicity in most sORFs, but mass spectrometry failed to detect peptides corresponding to the sORFs. Deletion of the entire ribosome-binding region of 24PHAS_NO296 locus eliminated ribosome binding and decreased 24-nt phasiRNA production, without affecting 24PHAS_NO296 transcript levels. In contrast, disrupting only the sORFs in 24PHAS_NO296 did not substantially affect the generation of 24-nt phasiRNAs. A newly formed sORF in these mutants may have re-directed ribosome binding to its transcripts. Overall, these findings demonstrate that sORFs facilitate ribosome binding to 24-PHAS transcripts, thereby promoting phasiRNA biogenesis in meiotic anthers.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"161 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142488634","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

EMBRYONIC FLOWER 1 regulates male reproduction by repressing the jasmonate pathway downstream transcription factor MYB26 EMBRYONIC FLOWER 1 通过抑制茉莉酸盐通路下游转录因子 MYB26 来调节雄性繁殖

The Plant Cell Pub Date : 2024-10-22 DOI: 10.1093/plcell/koae287

Zhijuan Chen, Jing Lu, Xiaoyi Li, Danhua Jiang, Zicong Li

{"title":"EMBRYONIC FLOWER 1 regulates male reproduction by repressing the jasmonate pathway downstream transcription factor MYB26","authors":"Zhijuan Chen, Jing Lu, Xiaoyi Li, Danhua Jiang, Zicong Li","doi":"10.1093/plcell/koae287","DOIUrl":"https://doi.org/10.1093/plcell/koae287","url":null,"abstract":"The evolutionarily conserved Polycomb repressive complexes (PRC) mediate genome-wide transcriptional silencing and regulate a plethora of development, as well as environmental responses in multicellular organisms. The PRC2-catalyzed trimethylation of lysine 27 on histone H3 (H3K27me3) is recognized by reader-effector modules of PRC1 to implement gene repression. Here, we report that the Arabidopsis (Arabidopsis thaliana) H3K27me3 effector EMBRYONIC FLOWER 1 (EMF1) interacts with and constrains the R2R3 DNA binding transcription factor MYB26 by a eudicot-conserved motif in the stamen. MYB26 activates the transcription of two NAC domain genes, NAC SECONDARY WALL THICKENING PROMOTING FACTOR1 (NST1) and NST2, whose encoded proteins mediate anther secondary cell thickening in jasmonate (JA)-regulated stamen maturation. In this process, the transcriptional activity of MYB26 is negatively modulated by the JAZ-PRC repressive complex to precisely regulate the expression of NST1 and NST2. Disruption of EMF1 repression stimulates MYB26, leading to the excessive transcription of the two NAC genes and male sterility. Our results reveal a novel mechanism in polycomb-mediated gene silencing and illustrate that the plant Polycomb complex regulates stamen development by preventing the hypersensitivity of JA responses in male reproduction.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"14 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2024-10-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142487372","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correction to: Nitrate in 2020: Thirty Years from Transport to Signaling Networks. 更正：2020 年的硝酸盐：从运输到信号网络的三十年。

The Plant Cell Pub Date : 2024-10-22 DOI: 10.1093/plcell/koae265

引用次数: 0

Building barriers: The role of MYB genes in rice root adaptation. 建立障碍：MYB 基因在水稻根系适应性中的作用。

The Plant Cell Pub Date : 2024-10-22 DOI: 10.1093/plcell/koae284

Gwendolyn K Kirschner

引用次数: 0

Illuminating the future: Enhanced glowing plants achieved by rewiring metabolism. 照亮未来：通过重新连接新陈代谢实现植物发光。

The Plant Cell Pub Date : 2024-10-22 DOI: 10.1093/plcell/koae286

Andrew C Willoughby

引用次数: 0