The Plant Cell最新文献_第10页

The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803 连接蛋白ApcI调控Synechocystis sp. PCC 6803在红光下的光收获

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf144

Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld

{"title":"The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803","authors":"Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld","doi":"10.1093/plcell/koaf144","DOIUrl":"https://doi.org/10.1093/plcell/koaf144","url":null,"abstract":"Phycobilisomes are versatile cyanobacterial antenna complexes that harvest light energy to drive photosynthesis. They can adapt to various light conditions; for example, dismantling under high light to prevent photo-oxidation and arranging in rows under low light to increase light harvesting efficiency. Light quality also influences phycobilisome structure and function, as observed under far-red light exposure. Here, we describe a phycobilisome linker protein, ApcI (previously hypothetical protein Sll1911), expressed specifically under red light (620 nm) or upon chemically induced reduction of the plastoquinone pool. We characterized ApcI in Synechocystis sp. PCC 6803 using mutant analyses, phycobilisome binding experiments, and protein interaction studies. Deletion of apcI conferred high light tolerance on Synechocystis sp. PCC 6803 compared to the wild-type strain, leading to reduced energy transfer from phycobilisomes to the photosystems under high light. Binding experiments revealed that ApcI replaces the linker protein ApcG at the membrane-facing side of the phycobilisome core via a paralogous C-terminal motif. Additionally, the N-terminal region of ApcI interacts with photosystem II. Our findings highlight the importance of phycobilisome remodeling for adaptation to different light conditions. The characterization of ApcI provides insight into the mechanisms by which cyanobacteria optimize light harvesting in response to varying light conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips 拟南芥根尖tRNASer/tRNATyr基因簇的再激活

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf137

Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu

{"title":"Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips","authors":"Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu","doi":"10.1093/plcell/koaf137","DOIUrl":"https://doi.org/10.1093/plcell/koaf137","url":null,"abstract":"Plants maintain redundant tRNA genes (tDNA) in their nuclear genomes, but the significance, regulation, and functional roles of these genes remain poorly understood. A cluster of tandemly repeated tDNAs decoding serine and tyrosine (SYY cluster) is located on Arabidopsis (Arabidopsis thaliana) chromosome 1, intersecting constitutive heterochromatin and remaining transcriptionally silenced in most tissues. The natural conditions inducing transcription of these tDNAs remain unknown. Here, we elucidate the tissue-specific expression pattern of this cluster during seedling establishment. Our findings reveal that SYY cluster tRNAs are primarily produced in the root cap columella and adjacent root cap cells. Transcriptional reactivation of the SYY cluster occurs in these tissues despite high DNA methylation levels. Furthermore, we demonstrate that these cells accumulate high levels of a transgenic glycoprotein rich in serine, tyrosine, and proline, and that CRISPR/Cas9 deletion of the SYY cluster alters the accumulation and stability of the glycoprotein in these specific cells. Our work provides pioneering evidence of a developmental and cell-specific expression program for a plant tDNA. We offer insights into the putative role of specialized tDNAs in enhancing glycoprotein biosynthesis in protective tissues of the meristem.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato E3连接酶PIRE1的基因编辑微调活性氧的产生，以增强番茄的细菌抗病能力

The Plant Cell Pub Date : 2025-05-30 DOI: 10.1093/plcell/koaf049

Bardo Castro, Suji Baik, Megann Tran, Jie Zhu, Tianrun Li, Andrea Tang, Nathalie Aoun, Alison C Blundell, Michael Gomez, Elaine Zhang, Myeong-Je Cho, Tiffany Lowe-Power, Shahid Siddique, Brian Staskawicz, Gitta Coaker

{"title":"Gene editing of the E3 ligase PIRE1 fine-tunes reactive oxygen species production for enhanced bacterial disease resistance in tomato","authors":"Bardo Castro, Suji Baik, Megann Tran, Jie Zhu, Tianrun Li, Andrea Tang, Nathalie Aoun, Alison C Blundell, Michael Gomez, Elaine Zhang, Myeong-Je Cho, Tiffany Lowe-Power, Shahid Siddique, Brian Staskawicz, Gitta Coaker","doi":"10.1093/plcell/koaf049","DOIUrl":"https://doi.org/10.1093/plcell/koaf049","url":null,"abstract":"Reactive oxygen species (ROS) accumulation is required for effective plant defense. Accumulation of the Arabidopsis (Arabidopsis thaliana) NADPH oxidase respiratory burst oxidase homolog D (RBOHD) is regulated by phosphorylation of a conserved C-terminal residue (T912) leading to ubiquitination by the RING E3 ligase Pbl13-interacting RING domain E3 ligase (PIRE). Arabidopsis PIRE knockouts exhibit enhanced ROS production and resistance to the foliar pathogen Pseudomonas syringae. Here, we identified 170 PIRE homologs, which emerged in tracheophytes and expanded in angiosperms. We investigated the role of tomato (Solanum lycopersicum) PIRE homologs in regulating ROS production, RBOH stability, and disease resistance. Mutational analyses of residues corresponding to T912 in the tomato RBOHD ortholog, SlRBOHB, affected protein accumulation and ROS production in a PIRE-dependent manner. Using genome editing, we generated mutants in 2 S. lycopersicum PIRE (SlPIRE) homologs. SlPIRE1 edited lines (Slpire1) in the tomato cultivar M82 displayed enhanced ROS production upon treatment with flg22, an immunogenic epitope of flagellin. Furthermore, Slpire1 exhibited decreased disease symptoms and bacterial accumulation when inoculated with foliar bacterial pathogens P. syringae and Xanthomonas campestris. However, Slpire1 exhibited similar levels of colonization as wild type upon inoculation with diverse soil-borne pathogens. These results indicate that PIRE regulates RBOHs in multiple plant species and is a promising target for foliar disease control. This study also highlights the pathogen-specific role of PIRE, indicating its potential for targeted manipulation to enhance foliar disease resistance without affecting root-associated pathogenic interactions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"11 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144184117","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A nematode effector hijacks a host RBR-type E3 ubiquitin ligase to regulate NRC4 receptor-mediated plant immunity and facilitate parasitism 一种线虫效应物劫持宿主rbr型E3泛素连接酶来调节NRC4受体介导的植物免疫并促进寄生

The Plant Cell Pub Date : 2025-05-27 DOI: 10.1093/plcell/koaf125

Xin Qin, Jiarong Yu, Wenjun Hu, Chen Chen, Cong Chen, Yuqi Shi, Yuwen Jiang, Shuai Zeng, Jun Hu, Ruiyan Wang, Xiaohua Yang, Xuan Wang

{"title":"A nematode effector hijacks a host RBR-type E3 ubiquitin ligase to regulate NRC4 receptor-mediated plant immunity and facilitate parasitism","authors":"Xin Qin, Jiarong Yu, Wenjun Hu, Chen Chen, Cong Chen, Yuqi Shi, Yuwen Jiang, Shuai Zeng, Jun Hu, Ruiyan Wang, Xiaohua Yang, Xuan Wang","doi":"10.1093/plcell/koaf125","DOIUrl":"https://doi.org/10.1093/plcell/koaf125","url":null,"abstract":"The root-knot nematode Meloidogyne incognita is an obligate biotrophic pathogen that causes extensive losses to agriculture worldwide. Effectors secreted by the parasite play an essential role during nematode infection through suppressing plant innate immunity. Here, we identify and characterize a M. incognita effector designated as MiV86, which is secreted into plant cells and positively regulates nematode parasitism. We show that MiV86 interacts with RING finger protein 217 (NbRNF217), an RBR-type E3 ubiquitin ligase of Nicotiana benthamiana, which negatively regulates plant immunity in an enzymatic activity-dependent manner. Moreover, we demonstrate that NbRNF217 targets and ubiquitinates the helper nucleotide-binding leucine-rich repeat receptor protein NRC4, resulting in its relocation and degradation through the 26S proteasome and endosomal/vacuolar pathways. NbRNF217 regulates its homeostasis through self-catalyzed ubiquitination or external ubiquitination modifications, and we show that MiV86 inhibits the ubiquitination of NbRNF217 in planta without affecting its activity, thereby promoting the degradation of NRC4, which also contributes to the resistance of N. benthamiana against M. incognita. Our findings reveal a mechanism by which a nematode effector hijacks an E3 ubiquitin ligase to attenuate NRC4-mediated plant immunity, facilitating nematode parasitism.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"58 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144153383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The histone acetyltransferase GCN5 regulates floral meristem activity and flower development in Arabidopsis 组蛋白乙酰转移酶GCN5调控拟南芥花分生组织活性和花发育

The Plant Cell Pub Date : 2025-05-25 DOI: 10.1093/plcell/koaf135

Amangul Hawar, Wei Chen, Tao Zhu, Xin Wang, Jiaxin Liu, Shiqi Xiong, Toshiro Ito, Dijun Chen, Bo Sun

{"title":"The histone acetyltransferase GCN5 regulates floral meristem activity and flower development in Arabidopsis","authors":"Amangul Hawar, Wei Chen, Tao Zhu, Xin Wang, Jiaxin Liu, Shiqi Xiong, Toshiro Ito, Dijun Chen, Bo Sun","doi":"10.1093/plcell/koaf135","DOIUrl":"https://doi.org/10.1093/plcell/koaf135","url":null,"abstract":"The histone acetyltransferase GENERAL CONTROL NON DEREPRESSIBLE5 (GCN5) participates in various developmental processes in Arabidopsis (Arabidopsis thaliana). Notably, GCN5 ensures proper flower development, but the underlying mechanism remains unknown. Here, we show that during early flower development, GCN5 catalyzes histone acetylation at WUSCHEL (WUS) and CLAVATA3 (CLV3) chromatin, activating their expression. WUS and CLV3 are required for floral meristem (FM) maintenance. Furthermore, we demonstrate that the GCN5–ALTERATION/DEFICIENCY IN ACTIVATION 2 (ADA2) histone acetyltransferase module interacts with the Switch/Sucrose non-fermentable ATPase SPLAYED (SYD) to form a GCN5–ADA2b–SYD ternary complex. The cytokinin responsive type-B Arabidopsis response regulators (ARRs) recruit this ternary complex for WUS activation. During floral organogenesis, PERIANTHIA recruits the GCN5–ADA2b module for AGAMOUS activation, which promotes FM determinacy. GCN5 also activates KNUCKLES, which ensures the timely termination of FM activity. Moreover, GCN5 modulates the expression pattern of the B-class gene APETALA3 and promotes the expression of SUPERMAN and CRABS CLAW, which are required for FM determinacy, thereby safeguarding meristem determinacy and correct floral organ formation. Thus, our study demonstrates the indispensable role of GCN5 in establishing a permissive chromatin environment to regulate the key genes required for precise flower development.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"97 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144136755","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A Helping Hand: XND1-DOF4.6 Interaction Modulates Arabidopsis Drought Tolerance. XND1-DOF4.6互作调控拟南芥抗旱性

The Plant Cell Pub Date : 2025-05-25 DOI: 10.1093/plcell/koaf129

Leonard Blaschek

引用次数: 0

In darkness, remember the light: Chlamydomonas retains low- and high-light-induced acclimatory phenotypes in the dark. 在黑暗中，记住光：衣藻在黑暗中保持低光和高光诱导的同化表型。

The Plant Cell Pub Date : 2025-05-23 DOI: 10.1093/plcell/koaf130

Guy Levin

引用次数: 0

Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits 硝酸盐应答植物基因回路调控结构的保存与分化

The Plant Cell Pub Date : 2025-05-22 DOI: 10.1093/plcell/koaf124

Chao Bian, Gozde S Demirer, M Tufan Oz, Yaomin Cai, Sam Witham, G Alex Mason, Zhengao Di, Florian Deligne, Ping Zhang, Rachel Shen, Allison Gaudinier, Siobhan M Brady, Nicola J Patron

{"title":"Conservation and divergence of regulatory architecture in nitrate-responsive plant gene circuits","authors":"Chao Bian, Gozde S Demirer, M Tufan Oz, Yaomin Cai, Sam Witham, G Alex Mason, Zhengao Di, Florian Deligne, Ping Zhang, Rachel Shen, Allison Gaudinier, Siobhan M Brady, Nicola J Patron","doi":"10.1093/plcell/koaf124","DOIUrl":"https://doi.org/10.1093/plcell/koaf124","url":null,"abstract":"Plant roots dynamically respond to nitrogen availability by executing a signaling and transcriptional cascade resulting in altered plant growth that is optimized for nutrient uptake. The NIN-LIKE PROTEIN 7 (NLP7) transcription factor senses nitrogen and, along with its paralog NLP6, partially coordinates transcriptional responses. While the post-translational regulation of NLP6 and NLP7 is well established, their upstream transcriptional regulation remains understudied in Arabidopsis (Arabidopsis thaliana) and other plant species. Here, we dissected a known sub-circuit upstream of NLP6 and NLP7 in Arabidopsis, which was predicted to contain multiple multi-node feedforward loops suggestive of an optimized design principle of nitrogen transcriptional regulation. This sub-circuit comprises AUXIN RESPONSE FACTOR 18 (ARF18), ARF9, DEHYDRATION-RESPONSIVE ELEMENT-BINDING PROTEIN 26 (DREB26), Arabidopsis NAC-DOMAIN CONTAINING PROTEIN 32 (ANAC032), NLP6 and NLP7 and their regulation of NITRITE REDUCTASE 1 (NIR1). Conservation and divergence of this circuit and its influence on nitrogen-dependent root system architecture were similarly assessed in tomato (Solanum lycopersicum). The specific binding sites of these factors within their respective promoters and their putative cis-regulatory architectures were identified. The direct or indirect nature of these interactions was validated in planta. The resulting models were genetically validated in varying concentrations of available nitrate by measuring the transcriptional output of the network revealing rewiring of nitrogen regulation across distinct plant lineages.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"144 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122553","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Phosphoglycerate dehydrogenase is required for kernel development and defines a predominant serine synthesis pathway in maize. 磷酸甘油酸脱氢酶是玉米籽粒发育所必需的，是玉米丝氨酸合成的主要途径。

The Plant Cell Pub Date : 2025-05-22 DOI: 10.1093/plcell/koaf126

Ying Zhang,Ruoxuan Li,Daocan Zheng,Jinhong Zhao,Ke Qing,Rongrong He,Zhaoxing Ma,Jie Chen,Nianguo Xue,Xing Tian,Enqi Wang,Jiameng Xu,Yubin Li,Bao-Cai Tan,Zhipeng Zhou,Chengyuan Wang,Jiaqiang Dong

{"title":"Phosphoglycerate dehydrogenase is required for kernel development and defines a predominant serine synthesis pathway in maize.","authors":"Ying Zhang,Ruoxuan Li,Daocan Zheng,Jinhong Zhao,Ke Qing,Rongrong He,Zhaoxing Ma,Jie Chen,Nianguo Xue,Xing Tian,Enqi Wang,Jiameng Xu,Yubin Li,Bao-Cai Tan,Zhipeng Zhou,Chengyuan Wang,Jiaqiang Dong","doi":"10.1093/plcell/koaf126","DOIUrl":"https://doi.org/10.1093/plcell/koaf126","url":null,"abstract":"Serine functions as both a substrate for protein biosynthesis and a signaling molecule for growth and development. However, the mechanism remains poorly understood. Here, we cloned and functionally characterized the maize (Zea mays) gene Dek20, which encodes phosphoglycerate dehydrogenase1 (PGDH1), the rate-limiting enzyme in the phosphorylated pathway of serine biosynthesis (PPSB). The dek20(Ser282Leu) mutation disrupts the interaction between residues Ser282 and His284, leading to the release of His284, which subsequently binds NAD+/NADH to inhibit serine biosynthesis. Consequently, serine content decreases dramatically, and the cellular response to nutrient starvation is enriched in transcriptome analysis. Serine deficiency triggers tRNASer degradation and reduced translation elongation at serine codons. The stalled ribosomes activate General Control Nonderepressible 2 (GCN2) kinase, which affects the phosphorylation of eukaryotic initiation factor 2α (eIF2α) and ribosomal protein S6 kinase (S6K), furtherly inhibiting translation initiation. Consistent with these findings, polysome profiling and Ribo-seq analysis revealed a marked decrease in translation efficiency in dek20. Notably, proteins essential for storage compound biosynthesis and cell cycle progression exhibit reduced translation in dek20. Collectively, our findings reveal the primary serine biosynthesis pathway and a mechanism for monitoring amino acid levels in maize, the model plant with C4 photosynthesis.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"193 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-05-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144122106","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pumping iron, keeping FIT: How MYB30 regulates FIT stability during plant iron deficiency. 抽铁，保持FIT: MYB30如何调节植物缺铁时FIT的稳定性。

The Plant Cell Pub Date : 2025-05-22 DOI: 10.1093/plcell/koaf134

Linhan Sun

引用次数: 0