The Plant Cell最新文献_第10页

Connecting the nucleus to the tip: A protein module linking RSL4 transcription with ROP signaling in root hair growth. 连接细胞核到根尖：一个连接根毛生长中RSL4转录与ROP信号的蛋白质模块。

The Plant Cell Pub Date : 2026-03-27 DOI: 10.1093/plcell/koag097

Luciano M Di Fino

引用次数: 0

A dynamic phospho-switch that times diurnal stomatal movements in Arabidopsis. 拟南芥中调节气孔运动的动态磷开关。

The Plant Cell Pub Date : 2026-03-27 DOI: 10.1093/plcell/koag094

Xinyu Zhang

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2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings 2-Cys过氧化物氧化酶和伴侣cpHSP70在拟南芥幼苗叶绿体生物发生中协同作用

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag096

Fernando Rodríguez-Marín, Antonia M Gallardo-Martínez, M Luisa Hernández, María-Cruz González, Francisco J Cejudo, Juan M Pérez-Ruiz

{"title":"2-Cys peroxiredoxins and the chaperone cpHSP70 act in concert in chloroplast biogenesis in Arabidopsis seedlings","authors":"Fernando Rodríguez-Marín, Antonia M Gallardo-Martínez, M Luisa Hernández, María-Cruz González, Francisco J Cejudo, Juan M Pérez-Ruiz","doi":"10.1093/plcell/koag096","DOIUrl":"https://doi.org/10.1093/plcell/koag096","url":null,"abstract":"The redox balance between NADPH-dependent thioredoxin reductase C (NTRC) and 2-Cys peroxiredoxins (PRXs) helps chloroplast photosynthetic performance acclimate rapidly to environmental cues. The Arabidopsis (Arabidopsis thaliana) 2cpab mutant, which lacks chloroplast 2-Cys PRXs A and B, shows impaired embryogenesis and cotyledon development. This phenotype indicates that these enzymes have a relevant function at early stages of plant development, although this function is poorly understood. Here, we show that the albino cotyledons of the 2cpab mutant have unstructured chloroplasts and decreased chloroplast lipid contents, revealing a key function of 2-Cys PRXs in cotyledon chloroplast differentiation. These phenotypes are mimicked by NTRC overexpression, whereas loss of NTRC function has no effect. RNA-Seq analyses showed transcriptomic changes resembling the response to proteotoxic stress in 2cpab seedlings, which was confirmed by the high sensitivity of 2cpab seedlings to heat stress. In de-etiolating seedlings 2-Cys PRXs interact with chloroplast-localized chaperone heat shock protein 70 (cpHSP70); moreover, Arabidopsis seedlings simultaneously lacking 2-Cys PRXs and cpHSP70-1 exhibit a dramatic alteration of seedling development. Based on these results, we propose that 2-Cys PRXs contribute to cotyledon chloroplast differentiation by affecting organellar proteostasis. This function is exerted in concert with chaperone cpHSP70 and is essential for seedling establishment.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"5 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524339","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RNHL1 phase separation coordinates ethylene and gibberellin signaling to regulate wheat plant height. RNHL1相分离协调乙烯和赤霉素信号传导调节小麦株高。

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag092

Hongwei Jing

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Spongy mesophyll cell death is induced by jasmonic acid during leaf senescence in chrysanthemum. 茉莉酸在菊花叶片衰老过程中诱导海绵状叶肉细胞死亡。

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag095

Jing Song,Yuming Zhang,Xiaoyue Hu,Zhengyao Shao,Aiping Song,Jiafu Jiang,Sumei Chen,Fadi Chen,Likai Wang

{"title":"Spongy mesophyll cell death is induced by jasmonic acid during leaf senescence in chrysanthemum.","authors":"Jing Song,Yuming Zhang,Xiaoyue Hu,Zhengyao Shao,Aiping Song,Jiafu Jiang,Sumei Chen,Fadi Chen,Likai Wang","doi":"10.1093/plcell/koag095","DOIUrl":"https://doi.org/10.1093/plcell/koag095","url":null,"abstract":"Leaf senescence impacts crop yield, quality, and post-harvest performance; for example leaf senescence limits the shelf life of cut flowers. Although the senescence process has been extensively studied at the whole-leaf level, its regulation at the single cell-type level remains largely unknown. Here, we tracked the cell death process during leaf senescence at the single cell-type resolution through integrated anatomical, multi-omics, molecular and genetic analyses in chrysanthemum (Chrysanthemum morifolium Ramat.). These analyses revealed that jasmonic acid (JA) induces spongy mesophyll cell death in the initial stage of leaf senescence, mediated by the transcription factors CmNAC055 and CmNAC087. These transcription factors regulate the acyl coenzyme A oxidase gene CmACX5, encoding a key JA biosynthetic enzyme. This regulatory module is functionally conserved across diverse species, including chrysanthemum, Arabidopsis thaliana, tomato (Solanum lycopersicum), and rice (Oryza sativa). Indeed, attenuating mesophyll cell death with a JA biosynthesis inhibitor delays tomato fruit de-greening and increases rice grain yield by approximately 6%. This study establishes a dynamic landscape of cell fate determination and hormonal regulation during leaf senescence, providing strategies to increase plant production by modulating leaf senescence.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147518549","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clearing the chromatin roadblock: A dual strategy for H2A.Z depletion under high temperature. 清除染色质障碍：H2A的双重策略。高温下的Z损耗。

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag093

Yu-Hung Hung

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The Marchantia polymorpha recombinase MpRAD51 is at the crossroads of morphogenesis and the DNA damage response Marchantia polymorpha重组酶MpRAD51处于形态发生和DNA损伤反应的十字路口

The Plant Cell Pub Date : 2026-03-26 DOI: 10.1093/plcell/koag089

Elias Trujillo-Esquivel, José Luis Lorenzo-Manzanarez, Annie Espinal-Centeno, Domingo Méndez-Alvarez, Adolfo Aguilar-Cruz, Luis G Brieba, Alfredo Cruz-Ramírez

{"title":"The Marchantia polymorpha recombinase MpRAD51 is at the crossroads of morphogenesis and the DNA damage response","authors":"Elias Trujillo-Esquivel, José Luis Lorenzo-Manzanarez, Annie Espinal-Centeno, Domingo Méndez-Alvarez, Adolfo Aguilar-Cruz, Luis G Brieba, Alfredo Cruz-Ramírez","doi":"10.1093/plcell/koag089","DOIUrl":"https://doi.org/10.1093/plcell/koag089","url":null,"abstract":"The transition of plants from water to land required major evolutionary innovations, including mechanisms to cope with environmental stress. DNA repair via the homologous recombination (HR) pathway is central in maintaining genome integrity, and its evolutionary conservation highlights its importance across plant lineages. Our analysis supports conservation of genes involved in the main activities for HR across the Viridiplantae. Among them, the recombinase radiation sensitive protein 51 (RAD51) has a central role in the HR pathway due to its DNA binding, homology recognition, and DNA displacement activities. Here we describe the functional characterization of the RAD51 ortholog in common liverwort (Marchantia polymorpha). In silico, the mutations within critical RAD51 domains and phosphorylation motifs identified offered insights into the possible regulatory adaptations of RAD51’s function through plant evolution. The main functions of Marchantia polymorpha RAD51 (MpRAD51) described in this pathway were confirmed biochemically. Nevertheless, beyond DNA repair, the loss of MpRAD51 showcased its role in the regulation of DNA-damage-induced plant development and differentiation, highlighting its importance in preventing growth arrest under genotoxic conditions. This study provides evidence of the evolutionary importance of HR for plant development and genome maintenance under both normal and genotoxic conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-26","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147524341","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Natural variation in the ORL4 promoter regulates rice leaf rolling to modulate leaf architecture and yield ORL4启动子的自然变异调控水稻叶片卷曲，从而调节叶片结构和产量

The Plant Cell Pub Date : 2026-03-20 DOI: 10.1093/plcell/koag086

Xianpeng Wang, Bingxia Xu, Qianfeng Hu, Yong Zhao, Quan Zhang, Honglin Li, Zhiqi Ma, Penghui Xu, Lingling Ma, Zhenyuan Wu, Xuehan Qian, Jinlong Li, Jinyue Yang, Ruiqi Liu, Wensheng Wang, Jianlong Xu, Junzhou Li, Lei Chen, Yinghua Pan, Xingming Sun, Jinjie Li, Hongliang Zhang, Zichao Li, Zhanying Zhang

{"title":"Natural variation in the ORL4 promoter regulates rice leaf rolling to modulate leaf architecture and yield","authors":"Xianpeng Wang, Bingxia Xu, Qianfeng Hu, Yong Zhao, Quan Zhang, Honglin Li, Zhiqi Ma, Penghui Xu, Lingling Ma, Zhenyuan Wu, Xuehan Qian, Jinlong Li, Jinyue Yang, Ruiqi Liu, Wensheng Wang, Jianlong Xu, Junzhou Li, Lei Chen, Yinghua Pan, Xingming Sun, Jinjie Li, Hongliang Zhang, Zichao Li, Zhanying Zhang","doi":"10.1093/plcell/koag086","DOIUrl":"https://doi.org/10.1093/plcell/koag086","url":null,"abstract":"Rice (Oryza sativa L.) leaf rolling enhances canopy architecture and photosynthetic efficiency, thereby improving yield potential. Through genome-wide association studies (GWAS) of 802 Xian and Geng rice accessions, we identified 32 loci associated with the leaf rolling index (LRI) and characterized OUTWARD ROLLED LEAF 4 (ORL4) as a regulatory gene from natural germplasm. CRISPR/Cas9-mediated knockout of ORL4 resulted in abaxially rolled leaves, driven by excessive bulliform cell proliferation and disrupted vascular patterning. A promoter variant (Chr04_19562737-C/T) modulates ORL4 expression by altering the binding affinity of the transcription factor RICE OUTERMOST CELL-SPECIFIC 8 (ROC8). Carriers of the ORL4C allele exhibit stronger ROC8 binding, higher ORL4 expression, and adaxially rolled or flat leaves; ORL4T carriers show the opposite phenotype. ORL4 forms a ternary complex with ROC8 and TOPLESS-RELATED PROTEIN 2(TPL2) to suppress ABAXIALLY CURLED LEAF 1(ACL1), a positive regulator of bulliform cell differentiation. Evolutionary analyses indicated that the ORL4C and ORL4T alleles originated from O. nivara and O. rufipogon, with ORL4C favored in Geng and ORL4T in Xian during domestication. Promoter editing of ORL4 in the elite cultivar ‘Shennong 89-366’ generated lines with enhanced LRI, achieving a significant yield increase in field trials. This study elucidates the genetic and molecular basis of leaf rolling and provides a promising strategy for rice yield improvement via precise promoter editing.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"15 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502136","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PtrSHR1 coordinates vascular cambium proliferation and xylem lignin biosynthesis to regulate wood formation in Populus trichocarpa PtrSHR1协调木质部木质素合成和维管形成层增殖，调控毛杨木材形成

The Plant Cell Pub Date : 2026-03-20 DOI: 10.1093/plcell/koag091

Jinfeng Zhao, Yanjie Chen, Xiufang Dai, Liwei Su, Rui Zhai, Yi Sun, Pingping Ji, Huiting Wang, Shuang Li, Chenguang Zhou, Yuki Tobimatsu, Toshiaki Umezawa, Vincent L Chiang, Wei Li

{"title":"PtrSHR1 coordinates vascular cambium proliferation and xylem lignin biosynthesis to regulate wood formation in Populus trichocarpa","authors":"Jinfeng Zhao, Yanjie Chen, Xiufang Dai, Liwei Su, Rui Zhai, Yi Sun, Pingping Ji, Huiting Wang, Shuang Li, Chenguang Zhou, Yuki Tobimatsu, Toshiaki Umezawa, Vincent L Chiang, Wei Li","doi":"10.1093/plcell/koag091","DOIUrl":"https://doi.org/10.1093/plcell/koag091","url":null,"abstract":"Vascular cambium produces cells to form xylem, or wood, in tree stems. Here we explored transregulatory pathways in this cell lineage development. We tested 20 of the 95 putative vascular cambium-specific (VCS) transcription factor (TF) genes through gain-of-function transgenesis in Black cottonwood (Populus trichocarpa) and found that a SHORT-ROOT (PtrSHR1) gene induced some of the most prominent phenotypes. PtrSHR1 transcripts are in the vascular cambium (VC) but not in the stem differentiating xylem (SDX), whereas PtrSHR1 proteins are in both VC and SDX. Constitutive overexpression or endonuclease-deficient Cas9 (dCas9) mediated loci-specific gene activation of PtrSHR1 revealed an activated PtrSHR1–PtrWRKY25–PtrVCS2–PtrWOX4a/b (WUSCHEL-related homeobox 4a/b) regulatory path for an abbreviated VC zone and a smaller stem diameter. CRISPR PtrSHR1 mutation reduced the PtrSHR1–PtrWRKY25–PtrVCS2–PtrWOX4a/b regulation resulting in an expanded VC zone and a larger stem diameter. In SDX, PtrSHR1–PtrWRKY94 activated specifically 3 (Ptr4CL3, 4-coumarate:CoA ligase 3; PtrC3H3, p-coumarate 3-hydroxylase 3; and PtrC4H1, cinnamate 4-hydroxylase 1) of the 22 monolignol biosynthetic pathway genes to control lignin content and structure, corroborated by chemical degradation and two-dimensional (2D) nuclear magnetic resonance (NMR) analyses. Thus, PtrSHR1 mediates the proliferation of VC and diffuses into SDX to control lignin properties and wood production, unveiling potential strategies for creating advantageous wood feedstock for materials and energy.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"59 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-03-20","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147502137","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The future is sweet: Exploiting sugar allocation and use for cotton breeding. 未来是甜蜜的：开发糖的分配和棉花育种利用。

The Plant Cell Pub Date : 2026-03-20 DOI: 10.1093/plcell/koag085

Pablo González-Suárez

引用次数: 0