The Plant Cell最新文献_第4页

The bifunctional transcription factor DEAR1 oppositely regulates chlorophyll biosynthesis and degradation in tomato fruits 双功能转录因子DEAR1反向调控番茄果实中叶绿素的合成和降解

The Plant Cell Pub Date : 2025-06-27 DOI: 10.1093/plcell/koaf167

Yangang Pei, Xiaoqing He, Qihan Xue, Heng Deng, Weijie Xu, Chengpeng Yang, Mengbo Wu, Weihao Wang, Wanjia Tang, Wenyi Niu, Yidi Huang, Ronggao Gong, Mondher Bouzayen, Yiguo Hong, Mingchun Liu

{"title":"The bifunctional transcription factor DEAR1 oppositely regulates chlorophyll biosynthesis and degradation in tomato fruits","authors":"Yangang Pei, Xiaoqing He, Qihan Xue, Heng Deng, Weijie Xu, Chengpeng Yang, Mengbo Wu, Weihao Wang, Wanjia Tang, Wenyi Niu, Yidi Huang, Ronggao Gong, Mondher Bouzayen, Yiguo Hong, Mingchun Liu","doi":"10.1093/plcell/koaf167","DOIUrl":"https://doi.org/10.1093/plcell/koaf167","url":null,"abstract":"Chlorophyll metabolism plays a key role in fruit development and ripening in tomato (Solanum lycopersicum). In immature fruits, chlorophyll facilitates photosynthesis, thereby supplying energy for growth. In contrast, chlorophyll degradation is an integral feature of ripening in most tomato varieties. Understanding how developing tomato fruit balances chlorophyll levels provides important information for crop improvement. Here, we report that the APETALA2/Ethylene Response Factor (AP2/ERF) family transcription factor SlDEAR1 is a key bifunctional regulator that activates chlorophyll biosynthesis and represses chlorophyll degradation in tomato fruits. Knocking out SlDEAR1 reduced chlorophyll accumulation compared to wild type, downregulated the chlorophyll biosynthesis gene Protochlorophyllide oxidoreductase 1 (SlPOR1), and upregulated the chlorophyll degradation gene Stay-green 1 (SlSGR1). SlDEAR1 overexpression led to the opposite results. Moreover, SlDEAR1 recruits the TOPLESS 2 (TPL2)–Histone deacetylase 1(HDA1)/HDA3 complex through its C-terminal EAR motif to decrease histone acetylation along the SlSGR1 promoter, leading to transcriptional repression. Simultaneously, SlDEAR1 upregulates SlPOR1 via a previously unidentified RKK motif. Furthermore, we showed that SlDEAR1 expression is light-regulated and mediated by the upstream transcriptional activator ELONGATED HYPOCOTYL 5 (SlHY5). Thus, the SlHY5–SlDEAR1–SlPOR1/SlSGR1 module regulates chlorophyll accumulation during tomato fruit development. This study sheds light on the regulatory network controlling chlorophyll metabolism in tomato.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"46 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144503761","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity. APC/CCDC20驱动的KNL2泛素依赖性蛋白水解对着丝粒完整性和有丝分裂保真度至关重要。

The Plant Cell Pub Date : 2025-06-25 DOI: 10.1093/plcell/koaf164

Manikandan Kalidass,Venkata Ganesh Jarubula,Maryia Ratnikava,Jothipriya Ramakrishnan Chandra,Samuel Le Goff,Aline V Probst,Silvia Esposito,Klaus D Grasser,Astrid Bruckmann,Jérôme F Gagneux,Reinier F Prosée,Twan Rutten,Veit Schubert,Dmitri Demidov,Esther Lechner,Florian A Steiner,Pascal Genschik,Inna Lermontova

{"title":"Ubiquitin-dependent proteolysis of KNL2 driven by APC/CCDC20 is critical for centromere integrity and mitotic fidelity.","authors":"Manikandan Kalidass,Venkata Ganesh Jarubula,Maryia Ratnikava,Jothipriya Ramakrishnan Chandra,Samuel Le Goff,Aline V Probst,Silvia Esposito,Klaus D Grasser,Astrid Bruckmann,Jérôme F Gagneux,Reinier F Prosée,Twan Rutten,Veit Schubert,Dmitri Demidov,Esther Lechner,Florian A Steiner,Pascal Genschik,Inna Lermontova","doi":"10.1093/plcell/koaf164","DOIUrl":"https://doi.org/10.1093/plcell/koaf164","url":null,"abstract":"Kinetochores are large protein complexes that serve as attachment sites for spindle microtubules, ensuring proper chromosome segregation during cell division. KINETOCHORE NULL2 (αKNL2) is a key kinetochore protein required for the incorporation of the centromeric histone variant CENH3. The precise regulation of αKNL2 levels is crucial, but the molecular mechanisms controlling this process remain largely unexplored. In this study, we demonstrated that the Anaphase-Promoting Complex/Cyclosome (APC/C) mediates the ubiquitin-dependent proteolysis of αKNL2 during mitosis. Our findings revealed that αKNL2 accumulates in the presence of 26S proteasome inhibitors, and our yeast two-hybrid and proteomic screens showed that proteins from the ubiquitin-proteasome pathway interact with KNL2 in Arabidopsis (Arabidopsis thaliana) and nematode (Caenorhabditis elegans). Arabidopsis αKNL2 directly interacts with Anaphase-Promoting Complex subunit 10 (APC10) and Cell Division Cycle 20.1 (CDC20.1), two substrate recognition components of the APC/C. RNAi-mediated depletion of APC/C resulted in the accumulation and mislocalization of endogenous αKNL2. Additionally, mutation or deletion of the D-box1 region, or substitution of residues K336 and K339, impaired αKNL2 degradation. The expression of a proteasome-resistant αKNL2 variant in planta caused severe defects in growth, fertility, and mitotic division. These findings show that APC/CCDC20-mediated degradation of αKNL2 is critical for proper kinetochore function and centromere integrity.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"26 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488027","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Brassinosteroid signaling mediated by the OsIAA7-OsGSK2-OsBZR1 module regulates seed size in rice. 由OsIAA7-OsGSK2-OsBZR1模块介导的油菜素内酯信号调节水稻种子大小。

The Plant Cell Pub Date : 2025-06-25 DOI: 10.1093/plcell/koaf165

Ronghua Qiu,Jin Yang,Jiaqi Hou,Peng Yao,Huangzhuo Xiao,Yequn Wu,Daoyi Tu,Shiqi Ye,Xin Zhao,Xiaoci Ma,Yating Zhao,Tingyu Chen,Lijia Li

{"title":"Brassinosteroid signaling mediated by the OsIAA7-OsGSK2-OsBZR1 module regulates seed size in rice.","authors":"Ronghua Qiu,Jin Yang,Jiaqi Hou,Peng Yao,Huangzhuo Xiao,Yequn Wu,Daoyi Tu,Shiqi Ye,Xin Zhao,Xiaoci Ma,Yating Zhao,Tingyu Chen,Lijia Li","doi":"10.1093/plcell/koaf165","DOIUrl":"https://doi.org/10.1093/plcell/koaf165","url":null,"abstract":"Grain size profoundly influences crop yield. Therefore, elucidating the molecular mechanisms controlling crop grain size is of great importance. Here, we report that the early auxin-responsive gene AUXIN or INDOLE-3-ACETIC ACID 7 (OsIAA7) negatively regulates grain size in rice (Oryza sativa L.), as loss of OsIAA7 function leads to the development of larger and heavier grains. OsIAA7 is highly expressed in developing panicles and grains, and the eGFP-OsIAA7 fusion protein is localized to the nuclei. The OsIAA7-mediated regulation of grain size involves constraining cell division and elongation in the longitudinal direction, as well as cell elongation in the transverse direction of spikelet hull cells. Biochemical analyses demonstrate a physical interaction between OsIAA7 and GLYCOGEN SYNTHASE KINASE 3 (GSK3)/SHAGGY-LIKE KINASE 2 (OsGSK2), which enhances the OsGSK2-BRASSINAZOLE-RESISTANT 1 (OsBZR1) interaction, resulting in OsBZR1 phosphorylation and degradation. Functional loss of OsIAA7 increases 24-epibrassionolide (BL) sensitivity, while BL treatment reduces OsIAA7-HA stability, indicating its involvement in BR signaling. Genetic analyses support a strong genetic interaction between OsIAA7 and OsGSK2, with OsIAA7 acting upstream of OsGSK2. In summary, our findings reveal the OsIAA7-OsGSK2-OsBZR1 regulatory module as a mechanism controlling grain size in rice.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"635 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144488026","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Advancing ecological and evolutionary research in Arabidopsis: extending insights into model and non-model plants 推进拟南芥的生态和进化研究：扩展对模式和非模式植物的见解

The Plant Cell Pub Date : 2025-06-13 DOI: 10.1093/plcell/koaf151

María Verónica Arana, F Xavier Picó

{"title":"Advancing ecological and evolutionary research in Arabidopsis: extending insights into model and non-model plants","authors":"María Verónica Arana, F Xavier Picó","doi":"10.1093/plcell/koaf151","DOIUrl":"https://doi.org/10.1093/plcell/koaf151","url":null,"abstract":"Arabidopsis is regarded as the gold standard among plant systems because it has generated knowledge with translational potential across various disciplines. Nevertheless, the influence of less-explored fields within the Arabidopsis community, such as ecology and evolutionary ecology, has yet to be synthesized to emphasize their contributions to other plant disciplines. This essay summarizes current eco-evolutionary knowledge in Arabidopsis and highlights its potential to enrich the insights made by the Arabidopsis community as well as others working with other plant model and non-model systems. We underline the value of accession-based approaches but also highlight the importance for developing population-based approaches to understand how and where evolutionary change begins. Furthermore, we focus on the evolutionary value of phenotypic plasticity as necessary to comprehend the response of organisms to environmental changes. We also elaborate on conceptual and technical challenges to transcriptomic studies conducted in field conditions that evaluate gene function and gene effect on integrated phenotypes in natural environments. Overall, we believe that the development of joint eco-evolutionary and genetic research in Arabidopsis can help other plant species to develop as model systems, but the Arabidopsis community should also look at the advances of other emerging plant model systems.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"221 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144278237","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Polycomb Repressive Complex 2 facilitates the transition from heterotrophy to photoautotrophy during seedling emergence 多梳抑制复合体2促进了苗期从异养到光自养的过渡

The Plant Cell Pub Date : 2025-06-13 DOI: 10.1093/plcell/koaf148

Naseem Samo, María Guadalupe Trejo-Arellano, Lenka Gahurová, Alexander Erban, Alina Ebert, Quentin Rivière, Jiří Kubásek, Fatemeh Aflaki, Helena Hönig Mondeková, Armin Schlereth, Annick Dubois, Mingxi Zhou, Ondřej Novák, Jiří Šantrůček, Daniel Bouyer, Francois Roudier, Joachim Kopka, Iva Mozgová

{"title":"Polycomb Repressive Complex 2 facilitates the transition from heterotrophy to photoautotrophy during seedling emergence","authors":"Naseem Samo, María Guadalupe Trejo-Arellano, Lenka Gahurová, Alexander Erban, Alina Ebert, Quentin Rivière, Jiří Kubásek, Fatemeh Aflaki, Helena Hönig Mondeková, Armin Schlereth, Annick Dubois, Mingxi Zhou, Ondřej Novák, Jiří Šantrůček, Daniel Bouyer, Francois Roudier, Joachim Kopka, Iva Mozgová","doi":"10.1093/plcell/koaf148","DOIUrl":"https://doi.org/10.1093/plcell/koaf148","url":null,"abstract":"The seed-to-seedling transition represents a key developmental and metabolic switch in plants. Catabolism of seed storage reserves fuels germination and early seedling emergence until photosynthesis is established. The seed-to-seedling developmental transition is controlled by Polycomb repressive complex 2 (PRC2). However, the coordination of PRC2 activity and its contribution to transcriptional reprogramming during seedling establishment remain unknown. By analyzing H3K27me3 re-distribution and changes in gene transcription in the shoot and root tissues of heterotrophic and photoautotrophic Arabidopsis (Arabidopsis thaliana) seedlings, we reveal two phases of PRC2-mediated gene repression. The first phase is independent of light and photosynthesis and results in the irreversible repression of the embryo maturation program, marked by heterotrophy and reserve storage molecule biosynthesis. The second phase is associated with the repression of metabolic pathways related to germination and early seedling emergence, and H3K27me3 deposition in this phase is sensitive to photosynthesis inhibition. We show that preventing the transcription of the PRC2-repressed glyoxylate cycle gene ISOCITRATE LYASE promotes the vegetative phase transition in PRC2-depleted plants. Our findings underscore a key role of PRC2-mediated transcriptional repression in the coordinated metabolic and developmental switches that occur during seedling emergence and emphasizes the close connection between metabolic and developmental identities.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"8 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144288258","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pancentromere analysis of Allium species reveals diverse centromere positions in onion and gigantic centromeres in garlic 葱属植物的全着丝粒分析表明，洋葱的着丝粒位置不同，大蒜的着丝粒位置巨大

The Plant Cell Pub Date : 2025-06-10 DOI: 10.1093/plcell/koaf142

Kiyotaka Nagaki, Koichiro Ushijima, Takashi Akagi, Keisuke Tanaka, Hisato Kobayashi

{"title":"Pancentromere analysis of Allium species reveals diverse centromere positions in onion and gigantic centromeres in garlic","authors":"Kiyotaka Nagaki, Koichiro Ushijima, Takashi Akagi, Keisuke Tanaka, Hisato Kobayashi","doi":"10.1093/plcell/koaf142","DOIUrl":"https://doi.org/10.1093/plcell/koaf142","url":null,"abstract":"In eukaryotes, centromeres interact with the kinetochore for distribution of genetic information in cell division, yet their sequence and size are diverse among species. However, their position on chromosomes is considered to be conserved within a species. In this study, we analyzed the centromeres of three Allium species, namely, Welsh onion (Allium fistulosum), onion (Allium cepa), and garlic (Allium sativum) via pancentromere analysis and repetitive sequence analysis of centromeres and their neighborhoods and revealed their mobility, sequence organization, and size. Among the three species, Welsh onion and garlic had stable centromeres, but the onion centromere appeared to be polymorphic and frequently differed in position by up to 28.0 Mb among cultivars and between multiple individuals of the same cultivar. This mobility was stabilized by hybridization with Welsh onions. Furthermore, these three species have very different centromere sequence organization, including differences in the existence and maturity of centromeric satellites, and differences in centromere size, with Welsh onion having a centromere of 1.9 Mb, and garlic having a centromere of approximately 10.6 Mb, the largest of any organism with monocentric chromosomes analyzed to date. Our pancentromere analysis of these Allium species reveals the variation in sequence organization, size and position of this important chromosomal region.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"21 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144260006","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Core biological principles and tools stemming from basic Arabidopsis research 拟南芥基础研究的核心生物学原理和工具

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf141

Lucia C Strader, Tianyuan Chen, Xinnian Dong, David Edwards, Sridevi Sureshkumar, Sureshkumar Balasubramanian, Mauricio S Antunes, Lili Zebluim, Patarasuda Chaisupa, R Clay Wright

引用次数: 0

Regulation of glucosylceramide synthase and sphingolipid remodeling in the plant response to phosphate deficiency 植物对磷酸盐缺乏反应中葡萄糖神经酰胺合成酶和鞘脂重塑的调控

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf138

Bao Yang, Yan Peng, Guo Zhang, Ruifan Liu, Simin Hao, Yi Ren, Shaoping Lu, Xuemin Wang, Liang Guo

{"title":"Regulation of glucosylceramide synthase and sphingolipid remodeling in the plant response to phosphate deficiency","authors":"Bao Yang, Yan Peng, Guo Zhang, Ruifan Liu, Simin Hao, Yi Ren, Shaoping Lu, Xuemin Wang, Liang Guo","doi":"10.1093/plcell/koaf138","DOIUrl":"https://doi.org/10.1093/plcell/koaf138","url":null,"abstract":"Sphingolipids are important and abundant lipids in the plasma membrane, and their homeostasis plays a key role in plant growth, development, and stress responses. We previously found that non-specific phospholipase C4 (NPC4) hydrolyzes sphingophospholipids upon phosphate starvation. Here, we defined the downstream steps of sphingolipid remodeling by identifying glucosylceramide synthase (GCS) and its regulatory mechanisms in Arabidopsis thaliana. Phosphate deficiency induces the expression of GCS, and the encoded GCS enzyme mediates glucosylceramide biosynthesis. Down-regulation of GCS severely affects sphingolipid homeostasis and hinders plant growth under phosphate starvation. Accordingly, GCS over-expression promotes sphingolipid remodeling to maintain plant growth. In addition, PHOSPHATE STARVATION RESPONSE1 (PHR1), a key regulator of phosphate homeostasis, directly affects the expression of NPC4 and GCS to regulate sphingolipid remodeling during phosphate starvation. Together, these results identify the PHR1–NPC4–GCS module as a regulatory mechanism that fine-tunes sphingolipid homeostasis and reveal the importance of sphingolipid homeostasis in the plant response to phosphate deficiency.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"770 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803 连接蛋白ApcI调控Synechocystis sp. PCC 6803在红光下的光收获

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf144

Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld

{"title":"The linker protein ApcI regulates light harvesting under red light in Synechocystis sp. PCC 6803","authors":"Roberto Espinoza-Corral, Tomáš Zavřel, Markus Sutter, Chase H Leslie, Kunwei Yang, Warren F Beck, Jan Červený, Cheryl A Kerfeld","doi":"10.1093/plcell/koaf144","DOIUrl":"https://doi.org/10.1093/plcell/koaf144","url":null,"abstract":"Phycobilisomes are versatile cyanobacterial antenna complexes that harvest light energy to drive photosynthesis. They can adapt to various light conditions; for example, dismantling under high light to prevent photo-oxidation and arranging in rows under low light to increase light harvesting efficiency. Light quality also influences phycobilisome structure and function, as observed under far-red light exposure. Here, we describe a phycobilisome linker protein, ApcI (previously hypothetical protein Sll1911), expressed specifically under red light (620 nm) or upon chemically induced reduction of the plastoquinone pool. We characterized ApcI in Synechocystis sp. PCC 6803 using mutant analyses, phycobilisome binding experiments, and protein interaction studies. Deletion of apcI conferred high light tolerance on Synechocystis sp. PCC 6803 compared to the wild-type strain, leading to reduced energy transfer from phycobilisomes to the photosystems under high light. Binding experiments revealed that ApcI replaces the linker protein ApcG at the membrane-facing side of the phycobilisome core via a paralogous C-terminal motif. Additionally, the N-terminal region of ApcI interacts with photosystem II. Our findings highlight the importance of phycobilisome remodeling for adaptation to different light conditions. The characterization of ApcI provides insight into the mechanisms by which cyanobacteria optimize light harvesting in response to varying light conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"85 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144237184","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips 拟南芥根尖tRNASer/tRNATyr基因簇的再激活

The Plant Cell Pub Date : 2025-06-06 DOI: 10.1093/plcell/koaf137

Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu

{"title":"Reactivation of the tRNASer/tRNATyr gene cluster in Arabidopsis thaliana root tips","authors":"Guillaume Hummel, Priyanka Kumari, Chenlei Hua, Long Wang, Yan-Xia Mai, Nan Wang, Negjmedin Shala, Emir Can Kaya, Jean Molinier, Jia-Wei Wang, Chang Liu","doi":"10.1093/plcell/koaf137","DOIUrl":"https://doi.org/10.1093/plcell/koaf137","url":null,"abstract":"Plants maintain redundant tRNA genes (tDNA) in their nuclear genomes, but the significance, regulation, and functional roles of these genes remain poorly understood. A cluster of tandemly repeated tDNAs decoding serine and tyrosine (SYY cluster) is located on Arabidopsis (Arabidopsis thaliana) chromosome 1, intersecting constitutive heterochromatin and remaining transcriptionally silenced in most tissues. The natural conditions inducing transcription of these tDNAs remain unknown. Here, we elucidate the tissue-specific expression pattern of this cluster during seedling establishment. Our findings reveal that SYY cluster tRNAs are primarily produced in the root cap columella and adjacent root cap cells. Transcriptional reactivation of the SYY cluster occurs in these tissues despite high DNA methylation levels. Furthermore, we demonstrate that these cells accumulate high levels of a transgenic glycoprotein rich in serine, tyrosine, and proline, and that CRISPR/Cas9 deletion of the SYY cluster alters the accumulation and stability of the glycoprotein in these specific cells. Our work provides pioneering evidence of a developmental and cell-specific expression program for a plant tDNA. We offer insights into the putative role of specialized tDNAs in enhancing glycoprotein biosynthesis in protective tissues of the meristem.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"42 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-06-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144236946","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0