The Plant Cell最新文献_第4页

A PP2A Phosphatase-Defined Transcriptional Activity Window Safeguard Male Fertility PP2A磷酸酶定义的转录活性窗口保护男性生育能力

The Plant Cell Pub Date : 2026-08-07 DOI: 10.1093/plcell/koag242

Zesen Lai, Zhenze Lei, Jianzheng Wang, Yeqiao Liu, Ying Fu, Fang Chang

{"title":"A PP2A Phosphatase-Defined Transcriptional Activity Window Safeguard Male Fertility","authors":"Zesen Lai, Zhenze Lei, Jianzheng Wang, Yeqiao Liu, Ying Fu, Fang Chang","doi":"10.1093/plcell/koag242","DOIUrl":"https://doi.org/10.1093/plcell/koag242","url":null,"abstract":"Faithful pollen wall formation requires tight control of both the amplitude and duration of tapetal transcriptional activity. However, how such dynamic restraint is achieved during anther development remains poorly understood. Here, we identify a phosphorylation-dependent regulatory module in which SBI-PP2A-C1/C5 constrains the functional activity window of the tapetal transcription factor bHLH089 to safeguard male fertility. PP2A-C1 and PP2A-C5 physically associate with bHLH089 and limit its transcriptional output by reducing bHLH089 phosphorylation and nuclear retention, thereby restricting its nuclear accumulation and effective activity. Genetic analyses reveal that precise PP2A-C1/C5 dosage is critical for pollen wall integrity, as both reduced PP2A-C function and PP2A-C1/C5 overexpression disrupt tapetal transcriptional homeostasis and compromise male fertility. Mechanistically, Ser28 and Ser58 function as conserved phosphorylation-dependent determinants of bHLH089 nuclear retention and are required for PP2A-C1/C5-sensitive regulation of bHLH089 activity. Together, these findings identify a phosphatase-mediated timing mechanism that integrates transcriptional feedback with post-translational control to constrain bHLH089-driven transcriptional amplification within a self-limiting tapetal activity window required for tapetal homeostasis, pollen wall formation, and male fertility.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"56 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148703436","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A PIF4-PUB9-DSK2A signaling loop integrates skotomorphogenesis and carbon starvation response in Arabidopsis PIF4-PUB9-DSK2A信号通路整合了拟南芥的形态形成和碳饥饿反应

The Plant Cell Pub Date : 2026-08-05 DOI: 10.1093/plcell/koag237

Fan Wei, Yifan Zhang, Jie Zhang, Dongbei Guo, Xuefeng Zhang, Han Wang, Mengling Feng, Maowei Wang, Qing Han, Xingguang Deng, Honghui Lin, Dawei Zhang

{"title":"A PIF4-PUB9-DSK2A signaling loop integrates skotomorphogenesis and carbon starvation response in Arabidopsis","authors":"Fan Wei, Yifan Zhang, Jie Zhang, Dongbei Guo, Xuefeng Zhang, Han Wang, Mengling Feng, Maowei Wang, Qing Han, Xingguang Deng, Honghui Lin, Dawei Zhang","doi":"10.1093/plcell/koag237","DOIUrl":"https://doi.org/10.1093/plcell/koag237","url":null,"abstract":"When seeds germinate under soil, the emerging seedlings undergo a period of darkness. Short-term darkness promotes skotomorphogenesis, whereas prolonged darkness leads to carbon starvation, triggering a transition from growth to autophagy-mediated survival. The molecular mechanism by which plants coordinate skotomorphogenesis and carbon starvation response remains unclear. Here, we show that the ubiquitin receptor protein DOMINANT SUPPRESSOR OF KAR2 (DSK2) negatively regulates skotomorphogenesis, and the E3 ubiquitin ligase PLANT U-BOX 9 (PUB9) interacts with and ubiquitinates DSK2 for proteasomal degradation during skotomorphogenesis, thereby inhibiting the carbon starvation response. Moreover, PUB9 is a direct target of PHYTOCHROME-INTERACTING FACTOR 4 (PIF4), which promotes PUB9 expression during skotomorphogenesis. Upon carbon starvation, PIF4 is primarily degraded via DSK2A-dependent autophagy, leading to reduced PUB9 expression and consequently weakened DSK2A degradation. This feedback loop enables plants to shift from skotomorphogenesis toward carbon starvation response. Collectively, our work reveals that plants coordinate skotomorphogenesis and carbon starvation through the PIF4-PUB9-DSK2A signaling loop, providing a molecular link between growth and survival under dark and carbon-limited conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"54 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-08-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148682503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The OsARF12 - bZIP23 module links auxin signaling to ABA-associated regulatory pathways to improve seed vigor in rice OsARF12 - bZIP23模块将生长素信号与aba相关的调控途径联系起来，以提高水稻种子活力

The Plant Cell Pub Date : 2026-07-28 DOI: 10.1093/plcell/koag177

Xiao-Hui Feng, Wei-Qing Wang, Ze-Qun Liu, Ding-Yi Xu, Cui-Fang Tang, Ming Ma, Song-Quan Song, Jian Zhang, Xian-Jun Song

{"title":"The OsARF12 - bZIP23 module links auxin signaling to ABA-associated regulatory pathways to improve seed vigor in rice","authors":"Xiao-Hui Feng, Wei-Qing Wang, Ze-Qun Liu, Ding-Yi Xu, Cui-Fang Tang, Ming Ma, Song-Quan Song, Jian Zhang, Xian-Jun Song","doi":"10.1093/plcell/koag177","DOIUrl":"https://doi.org/10.1093/plcell/koag177","url":null,"abstract":"Seed vigor in plants can refer to the overall capacity of seeds to germinate and affect seedling morphogenesis, which is a key agronomic trait indicator of seed quality. However, the underlying gene network mechanism is unclear. Here, we report an important role played by the coordination of abscisic acid (ABA) and auxin signaling in controlling rice seed vigor. The core ABA signaling component STRESS ACTIVATED PROTEIN KINASE 1 (SAPK1) is a positive modulator of the trait, which physically interacts with and phosphorylates the transcription factor bZIP23, and importantly, the phosphorylated bZIP23 exhibits stronger transactivation activity and improved seed vigor. Furthermore, OsARF12 directly binds to the promoter of bZIP23 and activates its expression. Quite coincidentally, our genetic and molecular experimental results verify that the OsTIR1-OsIAA10-OsARF12-centered auxin signaling is required for seed vigor control. Overall, our findings uncover the auxin signaling and SAPK1-bZIP23-mediated ABA signaling pathways, converging at the OsARF12-bZIP23 axis, to endow seeds with improved vigor in rice.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"20 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148596376","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Spirogyra pratensis genome illuminates the evolution of developmental programs and spiral chloroplast biology 草绵螺基因组阐明了发育程序和螺旋叶绿体生物学的进化

The Plant Cell Pub Date : 2026-07-25 DOI: 10.1093/plcell/koag224

Elisa S Goldbecker, Deepti Varshney, Anja Holzhausen, Tatyana Darienko, Hong Zhou, Armin Dadras, Lukas Pfeifer, Thomas Pröschold, Enrique Lopez-Gomez, Elke Woelken, Charlotte Permann, Tassilo Erik Wollenweber, Kerstin Becker, Stefanie König, Franz Hadacek, Fay-Wei Li, Ivo Feussner, Noe Fernandez-Pozo, Andreas Holzinger, Florian Maumus, André Marques, Henrik Buschmann, Klaus von Schwartzenberg, Jan de Vries, Stefan A Rensing

{"title":"The Spirogyra pratensis genome illuminates the evolution of developmental programs and spiral chloroplast biology","authors":"Elisa S Goldbecker, Deepti Varshney, Anja Holzhausen, Tatyana Darienko, Hong Zhou, Armin Dadras, Lukas Pfeifer, Thomas Pröschold, Enrique Lopez-Gomez, Elke Woelken, Charlotte Permann, Tassilo Erik Wollenweber, Kerstin Becker, Stefanie König, Franz Hadacek, Fay-Wei Li, Ivo Feussner, Noe Fernandez-Pozo, Andreas Holzinger, Florian Maumus, André Marques, Henrik Buschmann, Klaus von Schwartzenberg, Jan de Vries, Stefan A Rensing","doi":"10.1093/plcell/koag224","DOIUrl":"https://doi.org/10.1093/plcell/koag224","url":null,"abstract":"Zygnematophytes emerged as the unexpected closest algal relatives of land plants despite their simple body plans, raising questions about the morphogenetic toolkit present in the last common ancestor of land plants and algae. Genomic analyses have revealed that zygnematophytes are cellular giants, sharing homologous frameworks for several phytohormones, secondary metabolites, and key morphogenetic and transcriptional regulatory processes. Zygnematophytes fall into five orders, each of which has chartered its own evolutionary path. Here, we have sequenced a contiguous genome of Spirogyra pratensis, the eponymous representative of Spirogyrales and a classical model system for evolutionary cell biology in the green lineage. Building on this genome, we transcriptionally profiled the tractable life cycle of Spirogyra and its responses to a bifactorial gradient of light and temperature. Our data highlight the activation of quiescence and homeostatic programs. Yet what stands out most in Spirogyra is its spiral chloroplast—undulating intracellularly and abscising during mixed phragmoplast formation and furrowing. Leveraging the genome in tandem with co-expression network analyses, we describe the molecular underpinnings of the unique cytokinetic processes that govern both cell and plastid division. We find that Spirogyra deploys a molecular program characteristic of Phragmoplastophyta, yet lacks the deeply conserved plastid division machinery found in other archaeplastid plastids.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"35 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148596383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

JA-modulated OsbHLH091 drives lodicule cell expansion to initiate and synchronize diurnal floret opening in rice. ja调控的OsbHLH091驱动水稻小叶细胞扩增，启动和同步小花日开放。

The Plant Cell Pub Date : 2026-07-22 DOI: 10.1093/plcell/koag221

Tao Song,Nana Gu,Liangchao Wang,Mengjie Feng,Fudeng Huang,Yu Han,Heng Zhang,Yutong Liu,Min Zhou,Yuanyuan Hao,Hua Zhang,Fulin Wang,Xiaoting Li,Zhiping Deng,Jian Hua,Zhengge Zhu,Xiangyang Hu,Heng Xu,Ying Zhu

{"title":"JA-modulated OsbHLH091 drives lodicule cell expansion to initiate and synchronize diurnal floret opening in rice.","authors":"Tao Song,Nana Gu,Liangchao Wang,Mengjie Feng,Fudeng Huang,Yu Han,Heng Zhang,Yutong Liu,Min Zhou,Yuanyuan Hao,Hua Zhang,Fulin Wang,Xiaoting Li,Zhiping Deng,Jian Hua,Zhengge Zhu,Xiangyang Hu,Heng Xu,Ying Zhu","doi":"10.1093/plcell/koag221","DOIUrl":"https://doi.org/10.1093/plcell/koag221","url":null,"abstract":"Asynchronous floret opening hinders hybrid rice seed production, particularly between indica and japonica subspecies. Although jasmonate (JA) signaling regulates diurnal floret opening time (DFOT), the mechanism underlying structural changes in lodicule as a swellable organ driving floret opening remains unclear. Here, we identified OsbHLH091 as a lodicule-specific transcription factor mediating JA-induced floret opening. The null mutants of OsbHLH091 exhibit cleistogamy (closed flowers) without defects in floral organ, while OsbHLH091 overexpression accelerates floral opening. Mechanistically, OsbHLH091 activates cell expansion genes, including EXPANSINs and CELLULOSE SYNTHASEs, such activity is repressed by JAZ proteins, the JA signaling inhibitors. Rising JA before anthesis degrades JAZs, which releases OsbHLH091 to trigger lodicule expansion. Natural variation in OsbHLH091 contributes to DFOT differences between subspecies: the japonica haplotype interacts more strongly with JAZs, thus delaying opening. Together, as a lodicule-specific regulator, OsbHLH091 not only reveals a previously unknown mechanism controlling floret opening but also provides a precise tool to synchronize DFOT across subspecies, enabling efficient indica-japonica hybrid breeding.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"9 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148539105","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

A catalase-mediated redox-epigenetic switch governs rice immunity hijacked by a fungal effector 过氧化氢酶介导的氧化还原-表观遗传开关控制被真菌效应物劫持的水稻免疫

The Plant Cell Pub Date : 2026-07-18 DOI: 10.1093/plcell/koag219

Yuan Fang, Rui Wang, Yuhang Duan, Lili Wang, Deyao Zhang, Hong Hu, Hao Liu, Junbin Huang, Xiaolin Chen, Hsiang Tom, Guotian Li, Lu Zheng, Xiaoyang Chen

{"title":"A catalase-mediated redox-epigenetic switch governs rice immunity hijacked by a fungal effector","authors":"Yuan Fang, Rui Wang, Yuhang Duan, Lili Wang, Deyao Zhang, Hong Hu, Hao Liu, Junbin Huang, Xiaolin Chen, Hsiang Tom, Guotian Li, Lu Zheng, Xiaoyang Chen","doi":"10.1093/plcell/koag219","DOIUrl":"https://doi.org/10.1093/plcell/koag219","url":null,"abstract":"Pathogen-induced reactive oxygen species (ROS) act as key signaling molecules in plant immunity, but their integration with epigenetic regulation remains unclear. Here, we identify the rice (Oryza sativa) histone deacetylase OsHDA705 as a redox sensor that coordinates immunity through oxidative post-translational modifications (PTMs). Pathogen-induced ROS oxidizes OsHDA705 at cysteine 256 (C256), blocking its deacylase activity. This oxidation promotes hyperacylation of the transcription factor OsIPA1 and histones, thereby activating defense gene expression. We further show that the catalase OsCATB functions as a redox mediator, reducing oxidized OsHDA705 to restore its deacetylase activity, thereby re-establishing the suppression of immunity. The fungal pathogen Ustilaginoidea virens hijacks this process via the secreted effector UvSE1, which physically interacts with the host catalase OsCATB to boost its ROS-scavenging activity, thereby reducing the oxidation level of OsHDA705. Genetic disruption of the OsCATB-OsHDA705 module enhances broad-spectrum disease resistance. Our findings reveal a pathogen strategy to reprogram the host’s redox-epigenetic regulation and establish reversible histone deacetylase oxidation as a molecular switch regulating immune transcription in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476220","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

No longer splitting hairs: an integrative mathematical model of tip growth. 不再吹毛求疵：一个尖端生长的综合数学模型。

The Plant Cell Pub Date : 2026-07-18 DOI: 10.1093/plcell/koag214

Jan Hübbers

引用次数: 0

The E3 ligase BnNLA1 modulates seed coat color in Brassica napus through ubiquitination of BnC07MYB3a E3连接酶BnNLA1通过BnC07MYB3a泛素化调控甘蓝型油菜种皮颜色

The Plant Cell Pub Date : 2026-07-18 DOI: 10.1093/plcell/koag220

Ran Hu, Haijiang Liu, Yunshan Tang, Tao Wu, Chengxiang Li, Mengzhen Zhang, Bin Zhang, Huafang Wan, Huiyan Zhao, Nengwen Yin, Jiana Li, Kun Lu, Hao Yu, Benbo Xu, Lei Shi, Hai Du, Cunmin Qu

{"title":"The E3 ligase BnNLA1 modulates seed coat color in Brassica napus through ubiquitination of BnC07MYB3a","authors":"Ran Hu, Haijiang Liu, Yunshan Tang, Tao Wu, Chengxiang Li, Mengzhen Zhang, Bin Zhang, Huafang Wan, Huiyan Zhao, Nengwen Yin, Jiana Li, Kun Lu, Hao Yu, Benbo Xu, Lei Shi, Hai Du, Cunmin Qu","doi":"10.1093/plcell/koag220","DOIUrl":"https://doi.org/10.1093/plcell/koag220","url":null,"abstract":"In rapeseed (Brassica napus L.), excessive accumulation of flavonoid pigments (anthocyanins/proanthocyanidins) compromises the nutrition, flavor, and commercial value. Therefore, reducing the accumulation of seed coat pigments is one of the main objectives in B. napus breeding. To elucidate the mechanisms affecting the genetic architecture of seed coat color, a genome-wide association study (GWAS) of seed coat color was conducted with a diverse group of 393 B. napus cultivars. NITROGEN LIMITATION ADAPTATION1 (NLA1) was identified as a previously unrecognized regulator that controls seed coat color and participates in flavonoid biosynthesis and accumulation in B. napus. Increasing evidence suggests that BnNLA1 (BnA09NLA1/BnC08NLA1) interacts with and ubiquitinates BnC07MYB3a, an R2R3-MYB-type transcription factor and a candidate regulator of the seed coat color in B. napus, and the lysine residue K164 of BnC07MYB3a is the key ubiquitination. Both the BnA09NLA1/BnC08NLA1 knock-out (KO) mutants and overexpressing BnC07MYB3a lines in B. napus exhibit lighter seed coat color indicating lower anthocyanin and proanthocyanidin accumulation compared with the wild-type plants. BnC07MYB3a also directly binds to the promoter of the TRANSPARENT TESTA 6 (BnTT6) and BANYULS (BnBAN), and represses their expression in B. napus. As expected, the expression levels of BnTT6 and BnBAN are significantly reduced in the BnA09NLA1/BnC08NLA1 KO mutants compared to the wild-type plants. Our findings reveal a novel regulatory framework mediated by the BnNLA1-BnC07MYB3a module for controlling seed coat color in B. napus and provide a new strategy for breeding high-quality B. napus cultivars.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"156 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148476219","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The MYB transcription factor APL is a rational target for base editing to engineer flowering time. MYB转录因子APL是碱基编辑调控开花时间的合理靶点。

The Plant Cell Pub Date : 2026-07-15 DOI: 10.1093/plcell/koag215

Zhiwei Zhao,Huafeng Liang,Chengxiu Wu,Yifan Ni,Fei Wang,Fan Feng,Xin Zhang,Jingzhen Liu,Juan Yi,Xuan Du,Chunyi Zhang,Xiaoduo Lu,Yingjie Xiao,Jianbing Yan,Hongtao Liu

引用次数: 0

The regulatory cascade CtrMYC2-CtrMYB20-CtrSTP13 boosts sugar and JA accumulation for cold tolerance in Citrus. CtrMYC2-CtrMYB20-CtrSTP13调控级联促进柑橘耐寒性的糖和JA积累。

The Plant Cell Pub Date : 2026-07-15 DOI: 10.1093/plcell/koag216

Xiangming Shang,Lele Chu,Zhicheng Yang,Yilei Wang,Zeqi Zhao,Chao He,Haowei Chen,Zuolin Mao,Wenqiang Hu,Yike Zeng,Xi Zeng,Xin Jiang,Min Wang,Chunlong Li,Ji-Hong Liu

{"title":"The regulatory cascade CtrMYC2-CtrMYB20-CtrSTP13 boosts sugar and JA accumulation for cold tolerance in Citrus.","authors":"Xiangming Shang,Lele Chu,Zhicheng Yang,Yilei Wang,Zeqi Zhao,Chao He,Haowei Chen,Zuolin Mao,Wenqiang Hu,Yike Zeng,Xi Zeng,Xin Jiang,Min Wang,Chunlong Li,Ji-Hong Liu","doi":"10.1093/plcell/koag216","DOIUrl":"https://doi.org/10.1093/plcell/koag216","url":null,"abstract":"Jasmonic acid (JA) and soluble sugars are known to play critical roles in modulating cold tolerance in plants. However, the interplay between these signaling and metabolic pathways, as well as the transcriptional networks involved, remains poorly understood. In this study, we demonstrated that JA signaling was required for cold tolerance and for the cold-induced accumulation of soluble sugars in Citrus trifoliata. Integrated analyses of MeJA- and cold-treated transcriptomes identified CtrSTP13 as the most strongly induced sugar transporter gene under both conditions. CtrSTP13, localized to the plasma membrane and highly expressed in leaves, functioned as a hexose transporter for enhancing sugar accumulation and cold tolerance. We further revealed that the transcription factor CtrMYB20 was a direct positive regulator of CtrSTP13 expression, thereby controlling cold tolerance in a CtrSTP13-dependent manner. Additionally, CtrMYC2 acted as an upstream transcriptional activator of CtrMYB20 and positively regulated cold tolerance by indirectly promoting CtrSTP13-mediated sugar accumulation. Notably, overexpression of CtrSTP13 led to increased α-linolenic acid levels and upregulation of the JA biosynthetic gene CtrLOX3, and stimulated JA biosynthesis, whereas silencing CtrSTP13 produced opposite effects. Interestingly, knockdown of CtrLOX3 disrupted sugar-induced JA accumulation and impaired cold tolerance. Collectively, our results demonstrate that JA promotes cold-induced sugar accumulation through the transcriptional cascade CtrMYC2-CtrMYB20-CtrSTP13, while sugars feedback to enhance CtrLOX3-mediated JA biosynthesis, thereby establishing a regulatory loop that facilitates cold adaptation. These findings provide new insights into the elucidation of integrated JA signaling and sugar metabolism associated with plant cold stress responses.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148451704","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0