The Plant Cell最新文献_第5页

Conserved leaf–root metabolomic network asymmetry underpins divergent drought strategies 保守的叶根代谢组学网络不对称支持不同的干旱策略

The Plant Cell Pub Date : 2026-07-14 DOI: 10.1093/plcell/koag217

Mirza Shoaib, Simone J Rochfort, Priyanka Reddy, Doris Ram, Matthew J Hayden, Surya Kant

{"title":"Conserved leaf–root metabolomic network asymmetry underpins divergent drought strategies","authors":"Mirza Shoaib, Simone J Rochfort, Priyanka Reddy, Doris Ram, Matthew J Hayden, Surya Kant","doi":"10.1093/plcell/koag217","DOIUrl":"https://doi.org/10.1093/plcell/koag217","url":null,"abstract":"Plants orchestrate tissue-specific metabolic responses to osmotic stress, a major determinant of drought tolerance and crop productivity. Yet how leaf and root responses are coordinated to confer tolerance remains poorly understood. Here we show that drought tolerance in wheat is associated with a reproducible architectural asymmetry between tissue-level metabolomic correlation networks under controlled osmotic stress. In a drought-tolerant genotype, leaf networks are ∼40% denser and highly integrated, consistent with rapid photosynthetic adaptation, whereas root networks are modular and fragmented, consistent with localised responses. Temporal analysis revealed a decline in cross-tissue coordination, from early synchrony (ρ ≈ 0.546) toward greater tissue-specific organisation (ρ ≈ 0.350) under prolonged stress, a pattern absent in the susceptible genotype. Bayesian structure learning provided convergent support for these architectures as statistically robust, non-random network organisations (P &lt; 0.001). Our findings suggest that drought tolerance is associated with contrasting tissue-level correlation-network organisation and temporally structured leaf–root coordination under osmotic stress. This framework advances our understanding of stress adaptation and provides a conceptual basis for breeding climate-resilient crops by targeting key network properties.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"10 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440871","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Bifurcation of StCRY1-StHY5 axis orchestrates blue light-enhanced glycoalkaloid and chlorophyll accumulation in potato tubers stcry1 - sty5轴的分叉协调了蓝光增强的马铃薯块茎中糖生物碱和叶绿素的积累

The Plant Cell Pub Date : 2026-07-14 DOI: 10.1093/plcell/koag218

Jun Qin, Shenglin Jing, Shengxuan Liu, Huiling Zhang, Yingtao Zuo, Enshuang Wang, Xijuan Zhao, Jianke Dong, Peng Jiang, Xiaomeng Sun, Jin Wang, Shijie He, Qingwei Wang, Xiantao Ye, Xingkui Cai, Botao Song

{"title":"Bifurcation of StCRY1-StHY5 axis orchestrates blue light-enhanced glycoalkaloid and chlorophyll accumulation in potato tubers","authors":"Jun Qin, Shenglin Jing, Shengxuan Liu, Huiling Zhang, Yingtao Zuo, Enshuang Wang, Xijuan Zhao, Jianke Dong, Peng Jiang, Xiaomeng Sun, Jin Wang, Shijie He, Qingwei Wang, Xiantao Ye, Xingkui Cai, Botao Song","doi":"10.1093/plcell/koag218","DOIUrl":"https://doi.org/10.1093/plcell/koag218","url":null,"abstract":"Potato, the fourth largest food crop in the world, stores nutrients in underground tubers. However, light exposure induces tuber accumulation of chlorophyll and toxic steroidal glycoalkaloids (SGAs), an unwanted trait called tuber greening causes potato quality decline and parts of tuber inedible. Despite progress in enzymatic cascades governing SGAs biosynthesis, the regulatory scheme of SGAs and chlorophyll metabolism in light-exposed tubers persists as a critical knowledge gap. Here, we identify the blue light receptor StCRY1 plays a predominant role in light-induced tuber greening and SGAs elevation, which functions as a light-controlled transcriptional switch for genes involved in SGAs and chlorophyll biosynthesis. We show that transcription factor StHY5 acts downstream of StCRY1 to co-regulates both chlorophyll and SGAs metabolism. However, StMYB4, a transcription factor regulated by StHY5, promotes SGAs synthesis by directly binding to SGA biosynthetic genes without affecting chlorophyll homeostasis. Furthermore, StCRY1 employs a dual strategy by modulating StMYB4 expression and physically interaction with StMYB4 to regulate its transcriptional activity. Collectively, these findings uncover a modularly coordinated control of SGAs accumulation and chlorophyll biosynthesis by bifurcation of StCRY1-StHY5 axis, providing a promising strategy to concurrently curb light-induced tuber greening and glycoalkaloids accumulation.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440870","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The (un)likelihood of clock-driven lateral root priming; A modeling exploration 时钟驱动的横向根启动的（非）可能性；建模探索

The Plant Cell Pub Date : 2026-07-11 DOI: 10.1093/plcell/koag213

Kirsten H ten Tusscher

{"title":"The (un)likelihood of clock-driven lateral root priming; A modeling exploration","authors":"Kirsten H ten Tusscher","doi":"10.1093/plcell/koag213","DOIUrl":"https://doi.org/10.1093/plcell/koag213","url":null,"abstract":"Lateral root formation starts with priming, predisposing subsets of pericycle cells with the potential of future lateral root formation through semi-periodic elevations in auxin (signalling). While various mechanisms have been suggested, one frequently suggested hypothesis proposes a genetic, cell-autonomous root clock akin to the clock underlying vertebrate somitogenesis. Still, while gene expression variations were observed, so far this clock has not been proven. From a functional and evolutionary perceptive it is furthermore an open question whether lateral root priming is not more likely to arise from an emergent tissue level process similar to phyllotaxis. To help settle this debate in this study I use general knowledge of oscillator dynamics and simple models of auxin signalling to underline the unlikelihood of a root clock driving lateral root priming. I show how within a single cell oscillations are limited to a small parameter domain, with constraints intensifying due to the presence of multiple AUX/IAA and ARF types as well as auxin export. Furthermore, I demonstrate how non-meristem based oscillations, due to a lack of memory of oscillator phase, can not drive periodic prebranch site formation, and periodic inputs are insufficient to induce phase differences. Combined this underlines the unlikelihood of a root clock driving lateral root priming.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"152 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-11","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148440874","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Function behind choreography: cytoskeletal, nuclear, and mechanical dynamics drive growth transition in root hair development 舞蹈背后的功能：细胞骨架、细胞核和机械动力学驱动根毛发育的生长转变

The Plant Cell Pub Date : 2026-07-10 DOI: 10.1093/plcell/koag165

Gilles Dupouy, Tamsin Spelman, Gaurav Singh, Etienne Herzog, Stephanie Baudrey, Simone Bovio, Jérome Mutterer, Olivier Hamant, Alexandre Berr, Henrik Jönsson, Marie-Edith Chabouté

{"title":"Function behind choreography: cytoskeletal, nuclear, and mechanical dynamics drive growth transition in root hair development","authors":"Gilles Dupouy, Tamsin Spelman, Gaurav Singh, Etienne Herzog, Stephanie Baudrey, Simone Bovio, Jérome Mutterer, Olivier Hamant, Alexandre Berr, Henrik Jönsson, Marie-Edith Chabouté","doi":"10.1093/plcell/koag165","DOIUrl":"https://doi.org/10.1093/plcell/koag165","url":null,"abstract":"In plants, polar cell growth is essential for processes such as root hair and pollen tube growth, but how it integrates with nuclear movement, cytoskeletal organization, and cell mechanics is not fully understood. We conducted high-resolution live imaging of Arabidopsis thaliana root hairs throughout their development using a microfluidic device. We identified 3 distinct stages––fast growth, slow growth, and early maturation––and quantified growth kinetics at high temporal resolution. The transition from fast to slow growth was consistent with cytoskeletal dynamics causing reduced tip growth and decreased nucleus–tip distance. Based on these observations, we developed a mathematical model linking cytoskeletal dynamics with tip growth and nuclear dynamics. Through genetic and pharmacological approaches, we were able to disrupt or trigger this transition, supporting the model and revealing the existence of essential crosstalk between actin filaments and microtubules. Additionally, vacuole dynamics, root hair diameter, and cell stiffness changed during the fast-to-slow transition, indicating a coordinated regulation of multiple subcellular systems. Together, these results connect nuclear, cytoskeletal, and mechanical dynamics during root hair development, offering an integrated view of the subcellular processes behind the control of polar cell growth in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"319 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-10","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148415590","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Removing the smoke screen of SMAX1 and SMXL2's role in seedling growth. 揭开SMAX1和SMXL2在幼苗生长中作用的烟幕。

The Plant Cell Pub Date : 2026-07-08 DOI: 10.1093/plcell/koag209

Vanessica Jawahir

引用次数: 0

The RNA-binding dynamics of the exon-junction complex underlying splicing-coupled RNA fate determination in Arabidopsis. 拟南芥剪接偶联RNA命运决定的外显子连接复合体的RNA结合动力学。

The Plant Cell Pub Date : 2026-07-07 DOI: 10.1093/plcell/koag212

Kaiwen Sun,Fengli Zhao,Shuying Huang,Yifang Tan,Yongsheng Chang,Mingyi Bai,Danling Zhu,Zhe Wu

{"title":"The RNA-binding dynamics of the exon-junction complex underlying splicing-coupled RNA fate determination in Arabidopsis.","authors":"Kaiwen Sun,Fengli Zhao,Shuying Huang,Yifang Tan,Yongsheng Chang,Mingyi Bai,Danling Zhu,Zhe Wu","doi":"10.1093/plcell/koag212","DOIUrl":"https://doi.org/10.1093/plcell/koag212","url":null,"abstract":"The exon-junction complex represents a hallmark of protein-RNA packaging that is coupled with co-transcriptional RNA processing and influences the subsequent fate of RNA. Although being extensively studied in animals, the RNA-binding property and the function of EJC in plants remain elusive. Through eCLIP-seq, we found that the RNA-binding core of the EJC, eIF4AIII, is delivered to the -25 nt to -30 nt region upstream of exon-exon junctions in a splicing-dependent manner. eIF4AIII also binds the 3' end of introns, suggesting an intermediate binding state prior to exon-exon junction deposition. eIF4AIII binding strength correlates with the number of gene exons and is distinct across different genes. Inducible knockdown of eIF4AIII leads to widespread alteration of intron-retention, a small fraction of which is also regulated by HEN2 or UPF3, indicating roles for eIF4AIII in RNA decay and RNA splicing. Interestingly, CRISPR-mediated fragment deletion that introduces an exonic premature termination codon (PTC) leads to NMD independently of eIF4AIII, even when the EJC is deposited downstream of the PTC, indicating that the EJC is not compulsory for NMD in Arabidopsis. Overall, our study uncovers the RNA-binding landscape and novel functions of the exon-junction complex in plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400662","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

RIN4 fragments released by AvrRpt2 promote NDR1-dependent activation of RPS2. AvrRpt2释放的RIN4片段促进ndr1依赖性RPS2的激活。

The Plant Cell Pub Date : 2026-07-07 DOI: 10.1093/plcell/koag204

Ahmed J Afzal,Maheen Alam,Jianhua Huang,Moneeza Akbar Agha,Luis da Cunha,Muneeza Iqbal Rai,Jibran Tahir,Anmbreen Jamroze,He Zhao,Jonathan D G Jones,David Mackey

{"title":"RIN4 fragments released by AvrRpt2 promote NDR1-dependent activation of RPS2.","authors":"Ahmed J Afzal,Maheen Alam,Jianhua Huang,Moneeza Akbar Agha,Luis da Cunha,Muneeza Iqbal Rai,Jibran Tahir,Anmbreen Jamroze,He Zhao,Jonathan D G Jones,David Mackey","doi":"10.1093/plcell/koag204","DOIUrl":"https://doi.org/10.1093/plcell/koag204","url":null,"abstract":"Plant nucleotide-binding, leucine-rich-repeat (NLR) immune receptors recognize pathogen effectors and activate immunity. The NLR Resistance to Pseudomonas syringae2 (RPS2) recognizes Avirulence protein interacting with RPS2 (AvrRpt2), a Pseudomonas effector that promotes virulence by proteolytically cleaving a membrane-tethered host protein, RPM1-interacting protein 4 (RIN4). RIN4 cleavage by AvrRpt2 also activates RPS2. A model in which RPS2 is activated by elimination of RIN4 is consistent with the ectopic activity of RPS2 in plants lacking RIN4 but does not explain the link between AvrRpt2's virulence activity and RPS2 activation. We found that non-membrane-tethered RIN4 derivatives are potent cytosolic activators of RPS2. Activation of RPS2 by these RIN4 derivatives, like AvrRpt2-induced activation, and unlike ectopic activation in the absence of RIN4, requires the defense signaling protein NON-RACE-SPECIFIC DISEASE RESISTANCE 1 (NDR1). Cleavage products of RIN4 produced by AvrRpt2 play contrasting roles in the activation of RPS2, with the membrane-tethered C-terminal fragment suppressing RPS2 and the non-membrane-tethered internal fragment, dependent on compatibility with the C-terminal fragment, overcoming its suppression of RPS2.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"36 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148400663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The Plant RABC1 GTPase Coordinates with Exocyst Component SEC5A in Regulating ER-phagy under Endoplasmic Reticulum Stress. 植物RABC1 GTPase与胞囊成分SEC5A协同调节内质网胁迫下的er吞噬

The Plant Cell Pub Date : 2026-07-06 DOI: 10.1093/plcell/koag208

Shuai Hu,Dongmei Zhu,Xiaohui Ma,Linjuan Shi,Wenze Zhang,Mei Huang,Youshun Lin,Baiying Li,Yonglun Zeng,Liwen Jiang,Jinbo Shen

{"title":"The Plant RABC1 GTPase Coordinates with Exocyst Component SEC5A in Regulating ER-phagy under Endoplasmic Reticulum Stress.","authors":"Shuai Hu,Dongmei Zhu,Xiaohui Ma,Linjuan Shi,Wenze Zhang,Mei Huang,Youshun Lin,Baiying Li,Yonglun Zeng,Liwen Jiang,Jinbo Shen","doi":"10.1093/plcell/koag208","DOIUrl":"https://doi.org/10.1093/plcell/koag208","url":null,"abstract":"Small GTPase proteins regulate intracellular transport between endomembrane compartments, yet their roles in endoplasmic reticulum (ER) stress responses and selective autophagy remain poorly understood. Here we characterize the plant RAB GTPase RABC1 as a regulator of ER-phagy during ER stress. Our results demonstrate that rabc1 mutants are hypersensitive to heat shock and ER stress inducers dithiothreitol and tunicamycin. RABC1 localizes primarily to the ER and Golgi with partial trans-Golgi network (TGN) association. Upon ER stress, RABC1 is recruited to autophagosomes and subsequently delivered into the vacuole. Autophagic turnover of the ER chaperone Calnexin (CNX1)-GFP is impaired in the rabc1 mutant after DTT and TM treatments. Additionally, RABC1 interacts with the exocyst subunit SEC5A in planta, and this interaction is required for SEC5A recruitment to autophagosomes, promoting autophagosome formation. Double-mutant analysis indicates an additive genetic interaction between RABC1 and SEC5A in ER stress sensitivity. Taken together, our results suggest that RABC1 coordinates with SEC5A to promote autophagosome formation and ER-phagy during ER stress, revealing a novel mechanism by which a plant RAB GTPase regulate ER-phagy.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"41 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148388717","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

What fresh cell is this? Building a single-cell atlas of developing grass leaves in Brachypodium distachyon. 这是什么新鲜的细胞？建立短柄茅单细胞发育的草叶图谱。

The Plant Cell Pub Date : 2026-07-03 DOI: 10.1093/plcell/koag206

Róisín Fattorini

引用次数: 0

Graft transmissible resistance to Alternaria alternata is mediated by rootstock to scion JA transport activating raffinose synthesis 砧木对接穗JA转运激活棉子糖合成介导了移植物对互交黑穗病的遗传抗性

The Plant Cell Pub Date : 2026-07-03 DOI: 10.1093/plcell/koag210

Wenjie Li, Shanhu Zheng, Qingling Zhan, Yanyan Sun, Shuhuan Zhang, Yunxiao Guan, Ye Liu, Hongyu Ma, Charles W Melnyk, Shuang Zhao, Likai Wang, Zhenxing Wang, Jiafu Jiang, Sumei Chen, Zhiyong Guan, Fadi Chen

{"title":"Graft transmissible resistance to Alternaria alternata is mediated by rootstock to scion JA transport activating raffinose synthesis","authors":"Wenjie Li, Shanhu Zheng, Qingling Zhan, Yanyan Sun, Shuhuan Zhang, Yunxiao Guan, Ye Liu, Hongyu Ma, Charles W Melnyk, Shuang Zhao, Likai Wang, Zhenxing Wang, Jiafu Jiang, Sumei Chen, Zhiyong Guan, Fadi Chen","doi":"10.1093/plcell/koag210","DOIUrl":"https://doi.org/10.1093/plcell/koag210","url":null,"abstract":"Black spot disease, caused by the fungus Alternaria alternata, is a global plant pathogen that lacks sustainable control measures and poses a serious threat to multiple economically important crops. One approach to enhancing disease resistance in susceptible plants is grafting them onto disease-resistant rootstocks, yet the mechanisms by which roots enhance disease resistance in shoots remains largely unknown. Here, using chrysanthemum-Artemisia vulgaris grafts, we identified that a raffinose synthase-encoding gene, CmRS6, is essential in the susceptible chrysanthemum scion for graft-transmitted resistance from the disease-resistant A. vulgaris rootstock. Exogenous raffinose treatments enhanced A. alternata resistance in chrysanthemum, tomato, cabbage, and apple, highlighting its broad defensive role. The CmERF1B transcription factor activated CmRS6 expression and raffinose accumulation, whereas CmJAZ1-like repressed CmERF1B via direct interaction. While we found evidence for rootstock-to-scion transport of raffinose, long-distance JA transport from A. vulgaris rootstocks to chrysanthemum scions was the primary mechanism for graft-transmitted resistance, and A. alternata infection further promoted JA transport. Collectively, our study demonstrates that rootstock-to-scion JA transport mediates graft-transmitted A. alternata resistance by up-regulating scion raffinose biosynthesis, thereby offering new strategies for the sustainable control of A. alternata in crops.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"112 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-03","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148385251","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0