The Plant Cell最新文献_第6页

Photoexcited CRY1 physically interacts with ATG8 to regulate selective autophagy of HY5 and photomorphogenesis in Arabidopsis. 光激发CRY1与ATG8物理相互作用调节拟南芥中HY5的选择性自噬和光形态发生。

The Plant Cell Pub Date : 2025-08-08 DOI: 10.1093/plcell/koaf196

Lu Jiang,Shilong Zhang,Yuting Niu,Guangqiong Yang,Jiachen Zhao,Huishan Liu,Minyu Xiong,Lingyi Xie,Zhilei Mao,Tongtong Guo,Hong-Quan Yang,Wenxiu Wang

{"title":"Photoexcited CRY1 physically interacts with ATG8 to regulate selective autophagy of HY5 and photomorphogenesis in Arabidopsis.","authors":"Lu Jiang,Shilong Zhang,Yuting Niu,Guangqiong Yang,Jiachen Zhao,Huishan Liu,Minyu Xiong,Lingyi Xie,Zhilei Mao,Tongtong Guo,Hong-Quan Yang,Wenxiu Wang","doi":"10.1093/plcell/koaf196","DOIUrl":"https://doi.org/10.1093/plcell/koaf196","url":null,"abstract":"Cryptochromes (CRYs) are blue light photoreceptors that regulate various light responses in plants, including photomorphogenesis. Autophagy is a tightly controlled intracellular degradation pathway that plays a critical role in plant growth and development. CRY signaling inhibits the 26S proteasome-dependent degradation of LONG HYPOCOTYL 5 (HY5) through interactions with the CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-SUPPRESSOR OF PHYA-105 1 (SPA1) complex. However, whether CRY1 mediates the blue light-driven regulation of photomorphogenesis by regulating the autophagic degradation of HY5 remains unclear. Here, we show that CRY1 directly interacts with ATG8, a key player in selective autophagy, in a blue light-dependent manner in Arabidopsis (Arabidopsis thaliana). ATG8 and ATG5/ATG7 act genetically downstream of CRY1, but upstream of HY5, to regulate photomorphogenesis. In darkness, AUTOPHAGY-RELATED8 (ATG8) physically interacts with HY5 to facilitate its autophagic degradation and promote skotomorphogenesis. Under blue light, the CRY1-ATG8 interaction inhibits the ATG8-HY5 interaction, suppressing the nuclear export and co-localization of ATG8 and HY5 to the autophagosome, and HY5 degradation in the vacuole. This study reveals how CRY1-mediated blue light signaling regulates HY5 autophagy, which enables plants to fine-tune photomorphogenic development in response to light and nutrient availability.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"193 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144825627","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

PRC2 regulates cytokinin and HD-ZIP III pathways to orchestrate vascular tissue pattern formation in Arabidopsis. PRC2调控拟南芥细胞分裂素和HD-ZIP III通路，协调维管组织模式的形成。

The Plant Cell Pub Date : 2025-08-08 DOI: 10.1093/plcell/koaf194

Yufei Zhang,Runzhou Huang,Tingting Yang,An Li,Zihao Wang,Yuexin Wu,Yang Deng,Jing Zhang,Xin-Qiang He,Yue Zhou

{"title":"PRC2 regulates cytokinin and HD-ZIP III pathways to orchestrate vascular tissue pattern formation in Arabidopsis.","authors":"Yufei Zhang,Runzhou Huang,Tingting Yang,An Li,Zihao Wang,Yuexin Wu,Yang Deng,Jing Zhang,Xin-Qiang He,Yue Zhou","doi":"10.1093/plcell/koaf194","DOIUrl":"https://doi.org/10.1093/plcell/koaf194","url":null,"abstract":"Polycomb repressive complex 2 (PRC2) regulates plant development, but systemic PRC2 mutants have pleiotropic defects that complicate investigation of its role in regulating secondary growth. Here, we used CRISPR-TSKO to generate a vascular tissue-specific knockout of the PRC2 component FERTILIZATION INDEPENDENT ENDOSPERM (FIE) driven by the promoter of the vascular tissue-specific gene WUSCHEL HOMEOBOX RELATED 14 (WOX14), termed WOX14pro:FIE-KO, in Arabidopsis (Arabidopsis thaliana). WOX14pro:FIE-KO plants showed ectopic vascular bundles, vascular cylinders within the phloem parenchyma, and inhibited differentiation of reticulate or pitted vessels and fibers in xylem. RNA-seq and ChIP analyses revealed that FIE directly mediates H3K27me3 deposition on genes encoding isopentenyltransferase (IPT) cytokinin biosynthesis enzymes, repressing their transcription and modulating cytokinin's spatial distribution and responses in hypocotyls. Genetic manipulation of cytokinin biosynthesis or signal transduction partially rescued vascular defects in FIE-knockout hypocotyls. Furthermore, we identified HOMEODOMAIN LEUCINE ZIPPER CLASS III (HD-ZIPIII) transcription factors as downstream targets of the FIE-IPT module, showing reduced expression in WOX14pro:FIE-KO and restoration in WOX14pro:FIE/IPT3/IPT5/IPT7-KO. Overexpression of ARABIDOPSIS HOMEOBOX GENE 8 (ATHB8) in WOX14pro:FIE-KO also partially rescued the vascular defects. Collectively, our findings expand the application of tissue-specific knockout technology in plants and establish PRC2 and its downstream signaling cascade as regulators of vascular tissue patterning.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"186 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-08-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144825626","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination 晚间复合体蛋白拮抗ABI3和ABI5，暂时调控脱落酸信号和种子萌发

The Plant Cell Pub Date : 2025-07-30 DOI: 10.1093/plcell/koaf189

Ruyu Tang, Jiajia Yang, Xiao Han, Kunrong He, Cuiping Zhang, Milian Yang, Juping Zhang, Zhichong Huang, Jingwen Ye, Tingting Xu, Chunlan Yu, Jiancan Du, Qiantang Fu, Yanru Hu

{"title":"Evening complex proteins antagonize ABI3 and ABI5 to temporally regulate abscisic acid signaling and seed germination","authors":"Ruyu Tang, Jiajia Yang, Xiao Han, Kunrong He, Cuiping Zhang, Milian Yang, Juping Zhang, Zhichong Huang, Jingwen Ye, Tingting Xu, Chunlan Yu, Jiancan Du, Qiantang Fu, Yanru Hu","doi":"10.1093/plcell/koaf189","DOIUrl":"https://doi.org/10.1093/plcell/koaf189","url":null,"abstract":"Seed germination and post-germinative growth are precisely regulated by multiple signals. In Arabidopsis thaliana, the phytohormone abscisic acid (ABA) suppresses these processes and several circadian clock-associated proteins mediate ABA responses. Nevertheless, whether seed germination is controlled by temporal signals under diurnal conditions remains obscure, as do the associated underlying molecular mechanisms. Here, we found that the germination of wild-type seeds varies with time of release from cold stratification (i.e., transferred to 22°C) under diurnal conditions upon ABA, salinity or osmotic stress exposure. Additionally, the evening complex (EC) components EARLY FLOWERING 3 (ELF3), ELF4, and LUX ARRHYTHMO (LUX) attenuate ABA signaling. Notably, time-dependent seed germination relies on these EC components and other core clock proteins. ELF3, ELF4, and LUX physically interact with and act genetically upstream of ABSCISIC ACID INSENSITIVE3 (ABI3) and ABI5, two crucial transcriptional activators of ABA signaling. ELF3, ELF4, and LUX repress the function and accumulation of ABI3 and ABI5. Consistent with these results, ABI3 and ABI5 are essential for the time-based modulation of seed germination. Our findings highlight the critical effects of temporal signals on seed germination, and clarify the mechanism through which the EC components antagonize ABI3 and ABI5 to facilitate the crosstalk between the clock and ABA signaling pathways.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-30","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144747383","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Glutathionylation-mediated degradation of a cap-binding protein enhances Arabidopsis resistance to Plutella xylostella. 谷胱甘肽介导的帽结合蛋白降解增强拟南芥对小菜蛾的抗性。

The Plant Cell Pub Date : 2025-07-29 DOI: 10.1093/plcell/koaf188

Ning Lin,Hui Ye,Mengjie Zhao,Xingzhi Chen,Jing Ma,Chuanhong Wang,Tengyue Wang,Zhen Tao,Yibing Zhao,Qingyang Zhang,Jun Lai,Xinqiao Zhang,Jinghui Dong,Peijin Li

{"title":"Glutathionylation-mediated degradation of a cap-binding protein enhances Arabidopsis resistance to Plutella xylostella.","authors":"Ning Lin,Hui Ye,Mengjie Zhao,Xingzhi Chen,Jing Ma,Chuanhong Wang,Tengyue Wang,Zhen Tao,Yibing Zhao,Qingyang Zhang,Jun Lai,Xinqiao Zhang,Jinghui Dong,Peijin Li","doi":"10.1093/plcell/koaf188","DOIUrl":"https://doi.org/10.1093/plcell/koaf188","url":null,"abstract":"The lepidopteran insect pest diamondback moth (Plutella xylostella) causes severe yield losses in cruciferous plants worldwide; therefore, there is an urgent need to characterize the genes for resistance to P. xylostella in plants and decipher their mechanisms. We previously demonstrated that inactivating NOVEL CAP-BINDING PROTEIN (NCBP), also known as RESISTANCE TO PLUTELLA XYLOSTELLA (RPX1), enhanced Arabidopsis (Arabidopsis thaliana) resistance to P. xylostella larvae, and the larval infestation caused NCBP degradation. Here, we report that MYB30-INTERACTING WD40 PROTEIN 1 (MIW1), a component of the Cul4-RING ubiquitin ligase complex, interacts with NCBP and causes its degradation through the 26S proteasome pathway. Protein interaction, degradation, and site mutagenesis assays of NCBP indicate that the glutathione transferase GSTF2 also interacts with NCBP and promotes its glutathionylation, ubiquitination, and degradation. GSTF2 and glutathionylation of NCBP enhance the interaction between MIW1 and NCBP. Moreover, consistent with the roles of GSTF2 and MIW1 in P. xylostella resistance, gstf2 and miw1 mutants were sensitive to larval infestation, whereas transgenic Arabidopsis overexpressing GSTF2 and MIW1 were more resistant to the larvae. These findings demonstrate a role for glutathionylation in regulating 26S proteasome-mediated protein degradation in plant resistance to insect pests, thus revealing the functional mechanism of NCBP in this process.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"715 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737201","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The wheat transcription factor Q functions in gibberellin biosynthesis and signaling and regulates height and spike length 小麦转录因子Q参与赤霉素的生物合成和信号转导，调控穗高和穗长

The Plant Cell Pub Date : 2025-07-29 DOI: 10.1093/plcell/koaf183

Pan Liu, Shulin Xue, Jizeng Jia, Guangyao Zhao, Jie Liu, Yanzhen Hu, Cuizheng Kong, Dong Yan, Huan Wang, Xu Liu, Zefu Lu, Lifeng Gao

{"title":"The wheat transcription factor Q functions in gibberellin biosynthesis and signaling and regulates height and spike length","authors":"Pan Liu, Shulin Xue, Jizeng Jia, Guangyao Zhao, Jie Liu, Yanzhen Hu, Cuizheng Kong, Dong Yan, Huan Wang, Xu Liu, Zefu Lu, Lifeng Gao","doi":"10.1093/plcell/koaf183","DOIUrl":"https://doi.org/10.1093/plcell/koaf183","url":null,"abstract":"The Q gene is a key domestication gene in wheat (Triticum aestivum) that regulates free-threshing habit, spike morphology, height, and other critical agronomic traits. However, the precise molecular mechanisms underlying its function remain unclear. In this study, we identified a Q allele with a missense mutation (G to A) in the fifth exon of the Q gene, resulting in reduced plant height and spike length. Further investigation revealed that this mutation causes a Gly-229-Ser amino acid substitution, which enhances Q protein stability. Furthermore, we discovered that Q directly binds to the promoter region of Gibberellin 3-oxidase 2 gene (TaGA3ox2) and represses its expression. Moreover, Q interacts with both REDUCED HEIGHT1 (RHT1) and GIBBERELLIN INSENSITIVE 2 (TaGID2), which may disrupt GID2-triggered RHT1 degradation. Collectively, these findings reveal the dual roles of Q in regulating both GA biosynthesis and signaling, providing insights into the molecular mechanisms through which Q modulates plant height and spike length in wheat.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"6 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144737010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Defense strategies for plant health: disease resistance and tolerance. 植物健康防御策略：抗病性和耐受性。

The Plant Cell Pub Date : 2025-07-28 DOI: 10.1093/plcell/koaf186

Zhijuan Tang,Rui Mou,Guoyong Xu

{"title":"Defense strategies for plant health: disease resistance and tolerance.","authors":"Zhijuan Tang,Rui Mou,Guoyong Xu","doi":"10.1093/plcell/koaf186","DOIUrl":"https://doi.org/10.1093/plcell/koaf186","url":null,"abstract":"Emerging and re-emerging infectious diseases pose a major threat to wild plants and domesticated crops, a challenge intensified by increasing climate extremes and the rapid evolution of pathogen populations. To mitigate damage during pathogen-plant interactions, plants have evolved sophisticated defense strategies. Resistance, a well-established mechanism, enables plants to expel, contain, or kill invading pathogens. However, evidence from crop breeding and evolutionary studies highlights disease tolerance-where plants stay health despite infection, not through restricting pathogen proliferation-as an equally critical yet often overlooked strategy. Unlike resistance, tolerance may not impose strong selective pressure on pathogen populations, making it a more durable solution to disease management. This review examines the impact of infectious diseases on plant health and explores how resistance and tolerance mitigate disease-induced damage. We also discuss recent advances in uncovering the molecular, cellular, and genetic foundations of disease tolerance, including mechanisms such as clearing harmful protein condensates, activating autophagy, allocating resources and altering life-history traits. Furthermore, we explore how these insights can inform the development of disease-tolerant crops through breeding and biotechnology, ultimately supporting sustainable agriculture and enhancing global food security.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"284 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144720253","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential and challenges for application of microbiomes in agriculture 微生物组在农业中的应用潜力与挑战

The Plant Cell Pub Date : 2025-07-28 DOI: 10.1093/plcell/koaf185

Charles Copeland, Paul Schulze-Lefert, Ka-Wai Ma

{"title":"Potential and challenges for application of microbiomes in agriculture","authors":"Charles Copeland, Paul Schulze-Lefert, Ka-Wai Ma","doi":"10.1093/plcell/koaf185","DOIUrl":"https://doi.org/10.1093/plcell/koaf185","url":null,"abstract":"The plant microbiome can promote plant health and productivity through a multitude of mechanisms. Our understanding of plant microbiome interaction relies on descriptive natural surveys and experiments performed under simplified laboratory environments. While reductionist approaches are essential to understand mechanisms of plant-microbiome interactions, they risk missing emergent community properties seen in nature. To bridge the gap between basic research and real-world deployment of the microbiome for translational application, one has to consider both functional association as well as ecological principles governing interspecies and interkingdom interactions. In this review, we discuss the beneficial potential of plant microbiomes to enhance plant growth, nutrition, stress tolerance, pathogen protection and commercial value through the modulation of taste and flavors, using examples from both model plants and agriculturally important crops. We then discuss how microbial invasion and persistence in standing communities, tradeoffs under multiple stressors and community instability under host- and environment-imposed modulation should be considered in the rational design of microbial inocula, followed by a scrutiny of the method of microbial delivery. We synthesize ideas on how multiomic data including genomics, transcriptomes and metabolomics can be leveraged to identify strains or target genes of interest for functional studies, and how machine learning algorithms can be incorporated to enable prediction of plant microbiome interactions. Microbiome-based strategies hold promise for improvements in agriculture. Despite the intrinsic complexity of the underlying interactions, interdisciplinary approaches are constantly providing insight into microbiome functioning and assembly principles, which is key towards knowledge-based engineering of the microbiome for increased and sustainable crop performance.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144715359","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Harbingers of Bloom: Identifying the molecular mechanisms controlling fruit bloom formation in cucumbers. 开花的前兆：确定控制黄瓜果实开花形成的分子机制。

The Plant Cell Pub Date : 2025-07-28 DOI: 10.1093/plcell/koaf187

Róisín Fattorini

引用次数: 0

PlantConnectome: a knowledge graph database encompassing >71,000 plant articles. PlantConnectome：一个知识图谱数据库，包含bbb71,000篇植物文章。

The Plant Cell Pub Date : 2025-07-23 DOI: 10.1093/plcell/koaf169

Shan Chun Lim,Manoj Itharajula,Mads Harder Møller,Rohan Shawn Sunil,Kevin Fo,Yu Song Chuah,Herman Foo,Emilia Emmanuelle Davey,Melissa Fullwood,Guillaume Thibault,Marek Mutwil

{"title":"PlantConnectome: a knowledge graph database encompassing >71,000 plant articles.","authors":"Shan Chun Lim,Manoj Itharajula,Mads Harder Møller,Rohan Shawn Sunil,Kevin Fo,Yu Song Chuah,Herman Foo,Emilia Emmanuelle Davey,Melissa Fullwood,Guillaume Thibault,Marek Mutwil","doi":"10.1093/plcell/koaf169","DOIUrl":"https://doi.org/10.1093/plcell/koaf169","url":null,"abstract":"One of the main quests in plant biology is understanding how gene products and metabolites work together to form complex networks that drive plant development and responses to environmental stimuli. However, the ever-growing volume and diversity of scientific literature make it increasingly challenging to stay current with the latest advances in functional genetics studies. Here, we tackled this challenge by deploying the text-mining capacities of large language models to process over 71,000 plant biology abstracts. Our approach presents nearly five million functional relationships between 2.4 million biological entities-genes or gene products, metabolites, tissues, and others-with a high accuracy of over 85%. We encapsulated these findings in the user-friendly database PlantConnectome and demonstrated its diverse utility by providing insights into gene regulatory networks, protein-protein interactions, and stress responses. We believe this innovative use of AI in the life sciences will allow plant scientists to keep up to date with the rapidly growing corpus of scientific literature. PlantConnectome is available at https://plant.connectome.tools/.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"64 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2025-07-23","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144693211","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Forever green: A Temporal physiological and metabolic analysis reveals genetic drivers of the staygreen trait in maize. 永葆青春：一项时间生理和代谢分析揭示了玉米永葆青春性状的遗传驱动因素。

The Plant Cell Pub Date : 2025-07-23 DOI: 10.1093/plcell/koaf181

Christian Damian Lorenzo

引用次数: 0