The Plant Cell最新文献

{"title":"GIGANTEA shapes diurnal seedling growth by sequestering SMAX1 and SMXL2.","authors":"Suyeon Kim,Juyeon Park,Sooah Yun,Pil Joon Seo,Young-Joon Park","doi":"10.1093/plcell/koag205","DOIUrl":"https://doi.org/10.1093/plcell/koag205","url":null,"abstract":"The circadian clock regulates diurnal growth by integrating external signals and phytohormonal pathways in a time-specific manner. Our previous work demonstrated that the karrikin signaling components, SUPPRESSOR OF MAX2 1 (SMAX1) and SMAX1-LIKE2 (SMXL2), promote seedling growth by modulating the growth-promoting transcription factor PHYTOCHROME INTERACTING FACTOR 4 (PIF4)-mediated auxin signaling in Arabidopsis (Arabidopsis thaliana). However, the genetic and molecular links between the circadian clock and SMAX1/SMXL2 remain poorly understood. In this study, we show that the core clock protein GIGANTEA (GI) influences rhythmic growth by inhibiting the functions of SMAX1 and SMXL2. Our findings reveal that GI directly interacts with SMAX1 and SMXL2, thereby preventing their association with PIF4. This interaction leads to the destabilization of PIF4 protein and alters transcriptomic patterns, including those related to auxin signaling, ultimately suppressing hypocotyl elongation. Furthermore, under warm long-day conditions, elevated GI abundance enhances its interaction with SMAX1/SMXL2 during the night, thereby attenuating nighttime thermomorphogenic growth rhythms. We propose that the regulation of SMAX1 and SMXL2 by GI is essential for the precise modulation of growth rhythms in response to environmental changes.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"1 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-07-02","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148365617","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The gatekeeper: How PSY1 controls root growth.","authors":"Andrea Gómez-Felipe","doi":"10.1093/plcell/koag194","DOIUrl":"https://doi.org/10.1093/plcell/koag194","url":null,"abstract":"","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"72 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-29","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148342243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The CDC48A-PUX10 Complex Regulates Peroxisomal Protein Homeostasis via Ubiquitin-Dependent Degradation in Arabidopsis.","authors":"Hongwei Jing","doi":"10.1093/plcell/koag202","DOIUrl":"https://doi.org/10.1093/plcell/koag202","url":null,"abstract":"","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"29 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148335917","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Age-dependent epigenetic control of flavonoid metabolism underlies chemical defenses in ancient Ginkgo biloba.","authors":"Jinkai Lu,Yanbing Jiang,Bang Chang,Tongfei Wang,Shixiong Ren,Xi Zhang,Qingjie Wang,Jiawen Cui,Zhaogeng Lu,Sian Liu,Yadi Chen,Chunxiang Fu,Yingfang Zhu,Biao Jin,Jinxing Lin,Peng Liu,Richard A Dixon,Li Wang","doi":"10.1093/plcell/koag199","DOIUrl":"https://doi.org/10.1093/plcell/koag199","url":null,"abstract":"Ancient trees such as Ginkgo biloba exhibit remarkable longevity and sustained physiological vigor despite millennia of environmental stress, yet how aging reprograms their secondary metabolism and chemical defense remains unclear. Here, we investigated age-related epigenetic and metabolic changes across G. biloba individuals aged 1-1,070 years. We identified DEFICIENS AGAMOUS-LIKE 1 (GbDAL1) as a central age-associated regulator of flavonol metabolism. GbDAL1 expression rises progressively with age but is significantly reduced in juvenilized branches; this shift is driven by reduced expression of the DNA methyltransferase chromomethylase2 (GbCMT2), leading to promoter hypomethylation. Restoring GbCMT2 activity reinstates promoter DNA methylation and suppresses GbDAL1 transcription. Functionally, GbDAL1 negatively regulates flavonol biosynthesis by directly repressing the flavonol synthase (GbFLS) gene and inhibiting the transcriptional activity of GbMYBF1, thereby attenuating expression of flavonol pathway genes. Although total flavonol biosynthesis declines with age, metabolite profiling revealed marked accumulation of methylated and prenylated flavonols in ancient trees, suggesting an adaptive metabolic shift toward more stable defensive metabolites. These metabolites, together with diverse terpenoids, phenols and alkaloids, accumulate in the heartwood to form a persistent chemical barrier that supports long-term defense. Collectively, our findings reveal an epigenetically mediated age-metabolite regulatory axis in G. biloba, uncovering a molecular mechanism that links aging to secondary metabolic reprogramming and may contribute to the exceptional longevity and resilience of perennial plants.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"19 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148335916","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"OsOSCA2.4 regulates post-Golgi trafficking of storage proteins by modulating Ca2+ homeostasis in rice endosperm.","authors":"Yu Zhang,Yun Zhu,Pengcheng Zhang,Wang Tian,Hui Dong,Zebin Liu,Yu Chen,Xiuhao Bao,Tian Pan,Yehui Xiong,Xin Wang,Zhijie Ren,Xiaokang Jiang,Xiaohang Han,Ruonan Jing,Hongming Wu,Chuanwei Gu,Rongbo Chen,Xiaoli Chen,Jie Lei,Erchao Duan,Cailin Lei,Zhijun Cheng,Jiachang Wang,Yiqun Bao,Chuanyin Wu,Haiyang Wang,Yihua Wang,Yulong Ren,Jianmin Wan","doi":"10.1093/plcell/koag193","DOIUrl":"https://doi.org/10.1093/plcell/koag193","url":null,"abstract":"Seed storage proteins (SSPs), which accumulate specifically during seed development, constitute a major source of plant-derived protein in the human diet. Despite their critical role in determining crop quality, the molecular mechanisms underlying the intracellular trafficking of SSPs remain poorly understood. Here, we characterize the rice glutelin precursor accumulation16 (gpa16) mutant, which exhibits defective dense vesicle (DV)-mediated post-Golgi trafficking of proglutelins, resulting in their overaccumulation in the apoplast. GPA16 encodes OsOSCA2.4, a member of the reduced hyperosmolality-induced Ca2+ increase channel (OSCA) family. OsOSCA2.4 localizes to post-Golgi compartments including the trans-Golgi network (TGN) and prevacuolar compartment (PVC) in vegetative tissue, and DVs in the endosperm. The OsOSCA2.4(P397L) variant disrupts the formation of the Rab5a molecular module, a process influenced by intracellular Ca2+ homeostasis. Ca2+ imaging assays indicate that OsOSCA2.4 functions as a regulator of Ca2+ homeostasis of PVCs/DVs, the primary sites of Rab5a module activity. Genetic analyses reveal functional redundancy between OsOSCA2.4 and OsOSCA4.1 in modulating post-Golgi trafficking of proglutelins. Collectively, our findings identify OSCA-mediated Ca2+ homeostasis as a previously unrecognized regulatory layer governing post-Golgi trafficking of SSPs and uncover an unexpected role for OSCA proteins in vesicular trafficking in eukaryotes.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"145 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148336177","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Securing the replication ground: How barley stripe mosaic virus safeguards the host chloroplast envelope.","authors":"Ved Prakash","doi":"10.1093/plcell/koag195","DOIUrl":"https://doi.org/10.1093/plcell/koag195","url":null,"abstract":"","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"82 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148313858","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"CitNAC71-CitNAC76 coordinate cellulose and hemicellulose biosynthesis to regulate high-temperature-mediated granulation in citrus.","authors":"Chen Kang,Sijia Yang,Chenwen Zhou,Xuemin Zhang,Zimeng Yang,Yujia Li,Shaojia Li,Yue Wang,Qiong Lin,Dengliang Wang,Chenning Zhao,Xiaojuan Liu,Jinping Cao,Chongde Sun","doi":"10.1093/plcell/koag197","DOIUrl":"https://doi.org/10.1093/plcell/koag197","url":null,"abstract":"Juice sac granulation, a globally pervasive physiological disorder in citrus (Citrus spp.), leads to substantial economic losses annually. The disorder is characterized by the marked accumulation of cell wall components, yet the molecular mechanisms coordinating this process remain poorly understood. Here, we found that high temperature acts as a strong inducer of citrus granulation, triggering a rapid and pronounced accumulation of cellulose and hemicellulose in juice sacs-an early hallmark of the disorder. Through integrated transcriptomic, biochemical, and genetic analyses, we identified Cellulose synthase A 7/8 (CitCesA7/8) and IRREGULAR XYLEM 9/9H/14H (CitIRX9/9H/14H) as essential biosynthetic genes underlying this process. We further revealed that the NAM/ATAF/CUC (NAC) transcription factor CitNAC76 directly responds to high temperature and activates CitCesA7/8 and CitIRX9/9H/14H, acting as a central regulatory hub of cellulose and hemicellulose accumulation during granulation process. Notably, we uncovered a molecular 'brake', CitNAC71, which suppresses CitNAC76 expression and compromises its transactivation of CitCesA7/8 and CitIRX9/9H/14H, forming a dual-layered feedback module that fine-tunes cellulose and hemicellulose biosynthesis under heat stress. Together, our study unveiled the CitNAC71-CitNAC76 regulatory circuit that orchestrates stress-induced cell wall remodeling in citrus fruit, providing potential genetic targets for mitigating juice sac granulation and improving postharvest performance in a warming climate.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"22 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148322563","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"VvNUP96-VvTCP15 coupling regulates growth and salt tolerance via a VvmiR319c-VvTCP4 module in grapevine.","authors":"Longping Quan,Rongrong Dai,Chao Liu,Le Zheng,Jing Li,Yuanpeng Du,Zhen Gao","doi":"10.1093/plcell/koag198","DOIUrl":"https://doi.org/10.1093/plcell/koag198","url":null,"abstract":"Soil salinity is a major abiotic stress that limits grapevine (Vitis vinifera) growth and productivity. Understanding the molecular basis of salt tolerance is therefore critical for breeding stress-resilient cultivars. Here, we identify a Class I TCP transcription factor, VvTCP15, whose salt-induced expression promotes growth under both normal and saline conditions, whereas its knockdown reduces growth and increases salt sensitivity. Further investigation reveals that VvTCP15 confers growth promotion and enhanced stress resilience by directly binding to the VvMIR319c promoter and activating its transcription. In turn, VvmiR319c acts as a positive regulator by targeting and cleaving VvTCP4 mRNA, a Class II CIN-like TCP factor that functions as a negative regulator of growth under both conditions. We further show that the nucleoporin VvNUP96 physically interacts with VvTCP15 and promotes the formation of nuclear condensates through the intrinsically disordered region of VvTCP15. This interaction stabilizes VvTCP15 and enhances its transcriptional activation of VvMIR319c. Genetic analyses place VvNUP96 upstream of VvTCP15 in regulating growth and salt tolerance. Together, these results define a VvNUP96-VvTCP15-VvmiR319c-VvTCP4 regulatory module that coordinates growth and salt stress responses in grapevine. This work reveals a functional link between nucleoporins and transcription factors via phase-separated condensates and provides potential targets for improving crop performance under saline conditions.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"195 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148322561","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The mRNA covalent modification dihydrouridine regulates transcript turnover and photosynthetic capacity during plant abiotic stress","authors":"Li’ang Yu, Giovanni Melandri, Anna C Nelson Dittrich, Sebastian Calleja, Diep R Ganguly, Kyle Palos, Emily K Brewer, Hillary Fischer, Bruno Rozzi, Aparna Srinivasan, Toshihiro Obata, Hamada AbdElgawad, Gerrit T S Beemster, Riley Henderson, Ciara Garcia, Xiaodan Zhang, David Stern, Andrea Eveland, Eric Lyons, A Elizabeth Arnold, Aleksandra Skirycz, Susan J Schroeder, Brian D Gregory, Duke Pauli, Andrew D L Nelson","doi":"10.1093/plcell/koag196","DOIUrl":"https://doi.org/10.1093/plcell/koag196","url":null,"abstract":"RNA covalent modifications (RCMs) influence RNA stability and translation efficiency, and thus play critical roles in eukaryotic growth and development. However, their role in regulating plant performance under abiotic stress remains largely unexplored. Here, we integrated multi-omics data in six Sorghum bicolor accessions under water-limiting conditions in the field to explore the relationship between RCMs and drought response. Within a stress and photosynthesis-associated gene co-expression module, we identified SbDUS2, a member of a family of enzymes conserved across eukaryotes, that catalyzes the reduction of uracil to dihydrouridine (DHU) on RNA molecules. DHU-modified transcripts in this module were enriched for photosynthetic functions and showed strong correlation with photosynthetic traits. To elucidate the function of this RCM, we characterized loss of function dus2 mutants in Arabidopsis thaliana. Under control conditions, these DHU-deficient mutants exhibited impaired germination and delayed development. Furthermore, under heat or water-limiting conditions, these mutants showed significantly reduced net CO2 assimilation and survival. Using multiple transcriptome-wide RNA stability assays, we demonstrated that transcripts associated with lower DHU levels in a dus2 background generally exhibited increased stability compared to Col-0 controls. Particularly, lack of DUS2 led to the hyperstability of photosynthesis-related transcripts, impeding their turnover and likely preventing proper photosynthetic acclimation during stress. We propose a model where DHU acts as a critical post-transcriptional regulator marking mRNAs for rapid turnover under stress, highlighting an overlooked regulatory layer contributing to plant resilience.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"7 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-24","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148314045","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional specialization of two jasmonic acid-amido synthetases in rice defense and spikelet development.","authors":"Lei-Lei Li,Yujie Xiao,Yiqi Lin,Yumeng Chen,Xinzhang Song,Zheng Yuan,Yonggen Lou,Ran Li","doi":"10.1093/plcell/koag191","DOIUrl":"https://doi.org/10.1093/plcell/koag191","url":null,"abstract":"The phytohormone jasmonic acid (JA) regulates diverse aspects of plant growth, development, and defense, yet how a single hormone coordinates such varied outputs remains poorly understood. Central to jasmonate signaling is the biosynthesis and perception of bioactive JA-amino acid conjugates, such as JA-Ile, which are synthesized by JASMONATE RESISTANT (JAR) enzymes. Three OsJAR genes are present in the monocot crop rice. Here, we systematically dissect their functions using a complete set of CRISPR-Cas9-derived mutants. OsJAR1 and OsJAR2 redundantly maintain basal JA-Ile levels required for normal vegetative growth, whereas OsJAR3 is functionally attenuated due to low expression and weak enzymatic activity. Notably, OsJAR1 specifically mediates herbivore-induced JA-Ile biosynthesis and confers resistance to the major rice pest, the brown planthopper, under both laboratory and field conditions, whereas OsJAR2 is dispensable for defense. The expression of OsJAR1 is directly controlled by the MYC2-bHLH6 transcriptional cascade, forming a positive feedback loop that sustains JA signaling during herbivory. By contrast, OsJAR2, but not OsJAR1, is expressed during early inflorescence differentiation and contributes to JA-Ile accumulation at this stage, ensuring normal spikelet development. These findings reveal that functional specialization of OsJAR enzymes enables rice to precisely tailor JA responses to distinct developmental and environmental cues.","PeriodicalId":501012,"journal":{"name":"The Plant Cell","volume":"43 1","pages":""},"PeriodicalIF":0.0,"publicationDate":"2026-06-22","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148288247","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":0,"RegionCategory":"","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}