Polish Journal of Pathology最新文献_第3页

Crosstalk between OPG/RANKL/RANK in bone marrow mesenchymal stem cells and Wnt/b-catenin pathway in prostate cancer cells regulates bone metastasis of prostate cancer. 骨髓间充质干细胞OPG/RANKL/RANK与前列腺癌细胞Wnt/b-catenin通路的串扰调控前列腺癌骨转移。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.150029

Shihua Ye, Qiongyun Lin, Meihua Deng, Shulong Huang, Changlin Mao, Jiabin Zhang, Wuming Zhan, Guangbing Chen

{"title":"Crosstalk between OPG/RANKL/RANK in bone marrow mesenchymal stem cells and Wnt/b-catenin pathway in prostate cancer cells regulates bone metastasis of prostate cancer.","authors":"Shihua Ye, Qiongyun Lin, Meihua Deng, Shulong Huang, Changlin Mao, Jiabin Zhang, Wuming Zhan, Guangbing Chen","doi":"10.5114/pjp.2025.150029","DOIUrl":"https://doi.org/10.5114/pjp.2025.150029","url":null,"abstract":"This study aimed to investigate whether the crosstalk between the osteoprotegerin (OPG)/receptor activator of nuclear factor-kB (RANK)/receptor activator of nuclear factor-kB ligand (RANKL) in bone marrow mesenchymal stem cells (BMSCs) and Wnt/b-catenin pathways in Pca cells regulates bone metastasis of PCa. Our study showed that there was increased OPG/RANKL/RANK and b-catenin expression in the tissue of PCa and its bone metastasis. This study further showed that RANKL knockdown in BMSCs or b-catenin knockdown in PC-3s blocked the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. Conversely, RANKL overexpression in BMSCs and b-catenin overexpression in PC-3s promoted the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. These data indicate that the RANKL pathway in BMSCs promoted the PC-3s invasion and the catenin pathway in PC-3s activated BMSCs with expression of cancer-associated fibroblast markers, which promoted the bone metastasis. This suggests that the interaction and crosstalk between BMSCs in bone microenvironment and PCa play a critical role in the exquisite tropism for Pca bone metastasis. Cancer therapies classically target tumour cells; however, based on this study, targeting BMSCs in bone microenvironment is a reasonable option for PCa therapy strategy.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"25-37"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases. 激素受体阳性、人类表皮生长因子受体 2 阴性、腋窝结节病负担低的早期乳腺癌患者 Ki-67 增殖指数与肿瘤型 DX 复发评分的相关性 - 137 例病例回顾。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135859

Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili

{"title":"Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases.","authors":"Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili","doi":"10.5114/pjp.2024.135859","DOIUrl":"10.5114/pjp.2024.135859","url":null,"abstract":"The use of chemotherapy in breast cancer management has significantly contributed to the decrease in its mortality. Currently, the prognosis is determined by molecular biomarkers, such as oestrogen receptors, and human epidermal growth factor receptor 2. However, the increasing use of advanced molecular technologies, including oncotype DX recurrence score (ODX-RS), has provided the ability to estimate the risk of recurrence. Research has demonstrated that the ODX-RS helps to predict recurrence risk and the potential benefit of chemotherapy in breast cancer. As a result, it can assist clinicians in making decisions regarding using the chemotherapy. The goal of work is to explore the correlation between the ODX-RS and Ki-67 proliferative index (Ki-67-PI). This study included 137 patients with oestrogen positive, human epidermal growth factor receptor 2-negative early breast cancer, and had non- or early axillary disease. Patients with low Ki-67-PI were as follows: low ODX-RS in 17%, intermediate ODX-RS in 80%, and high ODX-RS in 2%. In the high Ki-67-PI group: low ODX-RS in 12%, intermediate ODX-RS in 48%, and high ODX-RS in 40%. In conclusion, the results show no significant correlation between the ODX-RS and Ki-67-PI (r = 0.511, p-value < 0.9).","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 1","pages":"8-18"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway. CTRP 9通过调节AMPK通路减轻OGD/R诱导的视网膜神经节细胞的凋亡和未折叠蛋白反应。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136025

Xiaofan Yang, Yuling Niu

{"title":"CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway.","authors":"Xiaofan Yang, Yuling Niu","doi":"10.5114/pjp.2024.136025","DOIUrl":"10.5114/pjp.2024.136025","url":null,"abstract":"C1q/TNF-related protein-9 (CTRP9) has been reported to play roles in several types of retinal diseases. However, the role and the potential mechanism of CTRP9 in glaucoma are still incompletely understood. The expression of CTRP9 in OGD/R-induced retinal ganglion cells (RGCs) was detected by quantitative real-time polymerase chain reaction and western blot assay. Cell proliferation was identified by cell counting Kit-8 assay. Flow cytometry, enzyme-linked immunosorbent assay and western blot assay were performed to assess cell apoptosis. Unfolded protein response (UPR), endoplasmic reticulum (ER) stress and the AMPK pathway were evaluated by western blot assay. The data showed that the expression of CTRP9 was significantly downregulated in OGD/R-induced 661W cells. OGD/R treatment reduced cell viability, promoted cell apoptosis and activated the UPR and ER stress. The overexpression of CTRP9 reversed the effects of OGD/R on 661W cell viability, apoptosis, the UPR and ER stress, as well as the AMPK pathway. However, Compound C, an inhibitor of AMPK signaling, reversed the protection of CTRP9 overexpression against injury from OGD/R in 661W cells. In summary, the results revealed that CTRP9 abated the apoptosis and UPR of OGD/R-induced RGCs by regulating the AMPK pathway, which may provide a promising target for the treatment of glaucoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 1","pages":"40-53"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions. 宫颈癌前病变中 PRMT5 和 DSG2 蛋白的预后潜力。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141221

Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov

{"title":"Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions.","authors":"Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov","doi":"10.5114/pjp.2024.141221","DOIUrl":"https://doi.org/10.5114/pjp.2024.141221","url":null,"abstract":"Precancerous cervical lesions are metaplastic alterations of epithelial cells of the cervix, eventually developing into cervical cancer. Despite primary and secondary prevention, the burden of cervical cancer remains high globally. Protein arginine methyltransferases (PRMT) represent post-translational modifications that interact with multiple signalling pathways, playing a role in epithelial-mesenchymal transition. In complex with desmoglein-2 (DSG2), a cell adhesion protein, both participate in the progression of dysplastic changes with potential malignant development. The presented study was performed on archival paraffin-embedded blocks from adult women. The studied samples were categorised into low-grade and high-grade intraepithelial lesions. Immunohistochemical analysis was used to observe subcellular localisation, immunoreaction intensity, and percentage of PRMT5- and DSG2-expressing cells, followed by statistical analysis. Preliminary results identified statistically significant differences between the expression and subcellular localisation of proteins in question in low-grade and high-grade squamous intraepithelial lesions. The primary goal of the presented study is to perceive the involvement of PRMT5 and DSG2 in the initiation and progression of cervical lesions. Our observations indicate the potential of the assessed proteins as prognostic markers. However, further studies of PRMT5 and DSG2 are required to provide greater insight into cervical carcinogenesis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"115-125"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Microinvasive lobular carcinoma arising in a benign phyllodes tumour - a short report and brief review of the literature. 良性蝶形花瘤中出现的微小浸润性小叶癌--简短报告和文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142085

Thomas Butters, Lynsey Williams, Charanjit Kaur, Zoltan Szollosi

引用次数: 0

Sebaceous vs. lipid-rich carcinoma of the breast. Clinicopathological characteristics and correlations - a report of two cases and a literature review. 乳腺皮脂癌与富脂癌。临床病理特征及相关性--两例病例报告及文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143114

Nikola Živković, Ana Cvetanović, Miloš Kostić, Maja Jovičić Milentijević, Miodrag Djordjević, Tijana Denčić, Julija Cvetković, Mirjana Ćuk

引用次数: 0

The role of the CLL-1 protein in disease monitoring in patients diagnosed with acute myeloid leukaemia and myelodysplastic syndrome. CLL-1 蛋白在急性髓性白血病和骨髓增生异常综合征患者疾病监测中的作用。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143228

Ewa Dobak, Sylwia Romana Jankowska-Szabłowska, Renata Guzicka-Kazimierczak, Paulina Poter, Elżbieta Urasińska, Katarzyna Karpińska-Łukaszewicz

引用次数: 0

Enhancers of cancer stem cells and epithelial-mesenchymal transition: Lin28, MUC1, and lipocalin-2 as a new prognostic axis in classical invasive lobular carcinoma of the breast. 肿瘤干细胞和上皮-间质转化的增强因子：Lin28、MUC1和脂钙素-2在典型浸润性乳腺小叶癌中作为新的预后轴

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.145812

Mohamed Alabiad, Raja Aljafil, Amany Shalaby, Ahmed M Yehia, Mohammed Alorini, Basma Hamed Ibrahim

{"title":"Enhancers of cancer stem cells and epithelial-mesenchymal transition: Lin28, MUC1, and lipocalin-2 as a new prognostic axis in classical invasive lobular carcinoma of the breast.","authors":"Mohamed Alabiad, Raja Aljafil, Amany Shalaby, Ahmed M Yehia, Mohammed Alorini, Basma Hamed Ibrahim","doi":"10.5114/pjp.2024.145812","DOIUrl":"10.5114/pjp.2024.145812","url":null,"abstract":"Breast carcinoma is one of the most common causes of cancer-related mortality among women worldwide. The primary objective of the present study was to eva-luate the expression of the epithelial-mesenchymal transition (EMT)-related markers Lin28, MUC1, and lipocalin-2 in invasive lobular carcinoma (ILC) and to investigate their correlation with clinicopathological characteristics and patient survival. This prospective cohort study included 120 classic ILC cases investigated for immunohistochemical expressions of Lin28, MUC1, and lipocalin-2 and followed them for five years or until death. The expression of markers in all tissue samples was assessed, analysed, and correlated with clinical-pathological parameters and outcomes. Lin28, MUC1, and lipocalin-2 were positively expressed in 55%, 75%, and 45%, respectively. The high expression of Lin28 and MUC1 and low lipocalin-2 were associated with poor clinicopathological characteristics and unfavourable overall survival. Lin28 and MUC1 were highly expressed in ILC and were associated with lower survival rates, poor outcomes, and a pessimistic prognosis in patients with ILC, while lipocalin-2 expression was associated with a positive outcome where its down-regulation was related to a poor prognosis in patients with ILC. Furthermore, we concluded that Lin28, MUC1, and lipocalin-2 could influence cancer behaviours, including proliferation, invasion, and migration, by regulating the EMT process and CSC criteria in ILC cells, making them potentially advantageous indicators and targeted treatments. Our research may have significant implications for understanding the pathophysiology and prognosis of ILC, which could help select treatment targets.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 4","pages":"274-286"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030216","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer. 下调的lncRNA LINC00908与胃癌的不良预后和恶性程度增加有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141283

Chunyue Zhang, Yanmin Zhang, Yan Wang

{"title":"Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer.","authors":"Chunyue Zhang, Yanmin Zhang, Yan Wang","doi":"10.5114/pjp.2024.141283","DOIUrl":"https://doi.org/10.5114/pjp.2024.141283","url":null,"abstract":"Long non-coding RNA LINC00908 is a functional biomarker in regulating tumour progression. Its dysregulation in gastric cancer implies its potential functional role. Few studies have noted the functional role of LINC00908 in gastric cancer. The potential of LINC00908 to serve as a biomarker in gastric cancer was evaluated. A total of 113 paired gastric cancer tissues and normal tissues were collected from patients with gastric cancer. LINC00908 levels were evaluated by polymerase chain reaction, and its significance in disease progression and patients' prognosis was assessed. In vitro, the function of LINC00908 in tumour-related cellular processes was evaluated with CCK8 and Transwell assay. Significant downregulation of LINC00908 was observed in gastric cancer and was negatively associated with disease development and overall survival of patients. LINC00908 showed significant inhibitory effects on the proliferation, migration, and invasion of gastric cancer cells. Additionally, miR-627-3p was sponged by LINC00908 and therefore mediated the function of LINC00908 in gastric cancer cells. LINC00908 functioned as a prognostic biomarker and tumour suppressor of gastric cancer, providing a therapeutic target for gastric cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"157-165"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of p16 protein in breast cancer. 乳腺癌中 p16 蛋白的表达。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136028

Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi

{"title":"Expression of p16 protein in breast cancer.","authors":"Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi","doi":"10.5114/pjp.2024.136028","DOIUrl":"10.5114/pjp.2024.136028","url":null,"abstract":"Breast cancer is the most common cancer and a leading cause of death in women in Saudi Arabia. P16 is a tumour suppressor gene that plays a crucial role in regulating cell cycle. Several studies have investigated the significance of p16 expression in various cancer types. However, the significance of p16 in breast cancer remains controversial and insufficiently studied. The present study aims to examine the association between p16 expression and clinicopathological factors in breast cancer using immunohistochemistry staining. The study utilised 475 prospectively collected tissue samples from 475 women with breast cancer in Saudi Arabia. Nuclear and cytoplasmic immunohistochemical staining of p16 was observed in 338 (71%) of the cases and showed significant direct associations with adverse tumour features, including high tumour grade (p < 0.0001), negative oestrogen receptor/progesterone receptor status (p < 0.001), and lymph node metastasis (p = 0.02). Our study revealed a significant association between p16 protein expression and the established negative prognostic parameters in breast carcinoma including tumour grade, lymph node metastasis, and oestrogen receptor and progesterone receptor status.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 1","pages":"19-24"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0