Polish Journal of Pathology最新文献

CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway. CTRP 9通过调节AMPK通路减轻OGD/R诱导的视网膜神经节细胞的凋亡和未折叠蛋白反应。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136025

Xiaofan Yang, Yuling Niu

{"title":"CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway.","authors":"Xiaofan Yang, Yuling Niu","doi":"10.5114/pjp.2024.136025","DOIUrl":"10.5114/pjp.2024.136025","url":null,"abstract":"C1q/TNF-related protein-9 (CTRP9) has been reported to play roles in several types of retinal diseases. However, the role and the potential mechanism of CTRP9 in glaucoma are still incompletely understood. The expression of CTRP9 in OGD/R-induced retinal ganglion cells (RGCs) was detected by quantitative real-time polymerase chain reaction and western blot assay. Cell proliferation was identified by cell counting Kit-8 assay. Flow cytometry, enzyme-linked immunosorbent assay and western blot assay were performed to assess cell apoptosis. Unfolded protein response (UPR), endoplasmic reticulum (ER) stress and the AMPK pathway were evaluated by western blot assay. The data showed that the expression of CTRP9 was significantly downregulated in OGD/R-induced 661W cells. OGD/R treatment reduced cell viability, promoted cell apoptosis and activated the UPR and ER stress. The overexpression of CTRP9 reversed the effects of OGD/R on 661W cell viability, apoptosis, the UPR and ER stress, as well as the AMPK pathway. However, Compound C, an inhibitor of AMPK signaling, reversed the protection of CTRP9 overexpression against injury from OGD/R in 661W cells. In summary, the results revealed that CTRP9 abated the apoptosis and UPR of OGD/R-induced RGCs by regulating the AMPK pathway, which may provide a promising target for the treatment of glaucoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases. 激素受体阳性、人类表皮生长因子受体 2 阴性、腋窝结节病负担低的早期乳腺癌患者 Ki-67 增殖指数与肿瘤型 DX 复发评分的相关性 - 137 例病例回顾。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135859

Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili

{"title":"Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases.","authors":"Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili","doi":"10.5114/pjp.2024.135859","DOIUrl":"10.5114/pjp.2024.135859","url":null,"abstract":"The use of chemotherapy in breast cancer management has significantly contributed to the decrease in its mortality. Currently, the prognosis is determined by molecular biomarkers, such as oestrogen receptors, and human epidermal growth factor receptor 2. However, the increasing use of advanced molecular technologies, including oncotype DX recurrence score (ODX-RS), has provided the ability to estimate the risk of recurrence. Research has demonstrated that the ODX-RS helps to predict recurrence risk and the potential benefit of chemotherapy in breast cancer. As a result, it can assist clinicians in making decisions regarding using the chemotherapy. The goal of work is to explore the correlation between the ODX-RS and Ki-67 proliferative index (Ki-67-PI). This study included 137 patients with oestrogen positive, human epidermal growth factor receptor 2-negative early breast cancer, and had non- or early axillary disease. Patients with low Ki-67-PI were as follows: low ODX-RS in 17%, intermediate ODX-RS in 80%, and high ODX-RS in 2%. In the high Ki-67-PI group: low ODX-RS in 12%, intermediate ODX-RS in 48%, and high ODX-RS in 40%. In conclusion, the results show no significant correlation between the ODX-RS and Ki-67-PI (r = 0.511, p-value < 0.9).","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions. 宫颈癌前病变中 PRMT5 和 DSG2 蛋白的预后潜力。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141221

Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov

{"title":"Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions.","authors":"Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov","doi":"10.5114/pjp.2024.141221","DOIUrl":"https://doi.org/10.5114/pjp.2024.141221","url":null,"abstract":"Precancerous cervical lesions are metaplastic alterations of epithelial cells of the cervix, eventually developing into cervical cancer. Despite primary and secondary prevention, the burden of cervical cancer remains high globally. Protein arginine methyltransferases (PRMT) represent post-translational modifications that interact with multiple signalling pathways, playing a role in epithelial-mesenchymal transition. In complex with desmoglein-2 (DSG2), a cell adhesion protein, both participate in the progression of dysplastic changes with potential malignant development. The presented study was performed on archival paraffin-embedded blocks from adult women. The studied samples were categorised into low-grade and high-grade intraepithelial lesions. Immunohistochemical analysis was used to observe subcellular localisation, immunoreaction intensity, and percentage of PRMT5- and DSG2-expressing cells, followed by statistical analysis. Preliminary results identified statistically significant differences between the expression and subcellular localisation of proteins in question in low-grade and high-grade squamous intraepithelial lesions. The primary goal of the presented study is to perceive the involvement of PRMT5 and DSG2 in the initiation and progression of cervical lesions. Our observations indicate the potential of the assessed proteins as prognostic markers. However, further studies of PRMT5 and DSG2 are required to provide greater insight into cervical carcinogenesis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of p16 protein in breast cancer. 乳腺癌中 p16 蛋白的表达。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136028

Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi

{"title":"Expression of p16 protein in breast cancer.","authors":"Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi","doi":"10.5114/pjp.2024.136028","DOIUrl":"10.5114/pjp.2024.136028","url":null,"abstract":"Breast cancer is the most common cancer and a leading cause of death in women in Saudi Arabia. P16 is a tumour suppressor gene that plays a crucial role in regulating cell cycle. Several studies have investigated the significance of p16 expression in various cancer types. However, the significance of p16 in breast cancer remains controversial and insufficiently studied. The present study aims to examine the association between p16 expression and clinicopathological factors in breast cancer using immunohistochemistry staining. The study utilised 475 prospectively collected tissue samples from 475 women with breast cancer in Saudi Arabia. Nuclear and cytoplasmic immunohistochemical staining of p16 was observed in 338 (71%) of the cases and showed significant direct associations with adverse tumour features, including high tumour grade (p < 0.0001), negative oestrogen receptor/progesterone receptor status (p < 0.001), and lymph node metastasis (p = 0.02). Our study revealed a significant association between p16 protein expression and the established negative prognostic parameters in breast carcinoma including tumour grade, lymph node metastasis, and oestrogen receptor and progesterone receptor status.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer. 下调的lncRNA LINC00908与胃癌的不良预后和恶性程度增加有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141283

Chunyue Zhang, Yanmin Zhang, Yan Wang

{"title":"Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer.","authors":"Chunyue Zhang, Yanmin Zhang, Yan Wang","doi":"10.5114/pjp.2024.141283","DOIUrl":"https://doi.org/10.5114/pjp.2024.141283","url":null,"abstract":"Long non-coding RNA LINC00908 is a functional biomarker in regulating tumour progression. Its dysregulation in gastric cancer implies its potential functional role. Few studies have noted the functional role of LINC00908 in gastric cancer. The potential of LINC00908 to serve as a biomarker in gastric cancer was evaluated. A total of 113 paired gastric cancer tissues and normal tissues were collected from patients with gastric cancer. LINC00908 levels were evaluated by polymerase chain reaction, and its significance in disease progression and patients' prognosis was assessed. In vitro, the function of LINC00908 in tumour-related cellular processes was evaluated with CCK8 and Transwell assay. Significant downregulation of LINC00908 was observed in gastric cancer and was negatively associated with disease development and overall survival of patients. LINC00908 showed significant inhibitory effects on the proliferation, migration, and invasion of gastric cancer cells. Additionally, miR-627-3p was sponged by LINC00908 and therefore mediated the function of LINC00908 in gastric cancer cells. LINC00908 functioned as a prognostic biomarker and tumour suppressor of gastric cancer, providing a therapeutic target for gastric cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumour budding in gallbladder carcinoma. 胆囊癌的肿瘤出芽。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135860

Luisa F Rivera-Moncada, Leonardo S Lino-Silva

引用次数: 0

Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel. 预后不良，结肠腺癌的免疫浸润与钙激活氯通道的低表达水平有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141232

Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu

{"title":"Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel.","authors":"Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu","doi":"10.5114/pjp.2024.141232","DOIUrl":"https://doi.org/10.5114/pjp.2024.141232","url":null,"abstract":"The calcium-activated chloride channel (CLCA4) in colon adenocarcinoma (COAD) and immunological infiltration have not been extensively studied. This work thoroughly employed several datasets to assess the expression, prognosis, and association between immune infiltration and clinicopathological characteristics of CLCA4 in cancer, as well as look into potential signalling pathways. The human protein atlas (HPA), TIMER, UALCAN, TISIDB, GSCA, SangerBox, GeneMANIA, and LinkedOmics were among the datasets that were used. The findings demonstrated that, in comparison to normal tissues, COAD tissues had lower levels of CLCA4 expression. The prognosis was worse for those whose levels of CLCA4 expression were lower. For validation, immunohistochemistry (HPA) was used. Positive correlations between CLCA4 mRNA expression and its copy number variation (CNV) were observed, and CLCA4 CNV was linked to immunological infiltration. Subsequent investigation demonstrated the association between immune cell markers, immune checkpoint genes, and immunological infiltration with CLCA4. The overall survival and disease-free survival of M0 patients were considerably better than those of M1 patients, and the groups with tumour stages M0 and M1 had notably different levels of CLCA4 expression. Its substantial enrichment in ion channel activity, transmembrane transporter activity, digestion, and other biological processes was revealed by gene ontology analysis. Oxidative phosphorylation, pancreatic secretion, Parkinson's and Alzheimer's diseases, renin secretion, and other signalling pathways were the primary associations found for CLCA4. It is evident that the immunological microenvironment and functions like ion transport, metabolism, and intestinal digestion are all impacted by CLCA4 expression.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal pulmonary bile embolism associated with acute pancreatitis - a case report and review of the literature. 与急性胰腺炎相关的致命性肺胆汁栓塞--病例报告和文献综述。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135762

Michał Knapik, Krzysztof Okoń, Magdalena Ulatowska-Białas

引用次数: 0

Spontaneous expulsion of a colorectal leiomyosarcoma. 结肠直肠细肌瘤自发排出。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.138588

Pierre de Mathelin, Felix Lerintiu, Jean-Baptiste Delhorme

引用次数: 0

Retrospective analysis of negative cytology results correlated with a positive high-risk in the human papillomavirus result and subsequent results of patients over a period of three years. 回顾性分析细胞学阴性结果与人类乳头瘤病毒阳性高风险结果的相关性，以及患者三年内的后续结果。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135828

Paweł Gerard Piotrowski, Michał Teodor Jeleń

引用次数: 0