Polish Journal of Pathology最新文献

CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway. CTRP 9通过调节AMPK通路减轻OGD/R诱导的视网膜神经节细胞的凋亡和未折叠蛋白反应。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136025

Xiaofan Yang, Yuling Niu

{"title":"CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway.","authors":"Xiaofan Yang, Yuling Niu","doi":"10.5114/pjp.2024.136025","DOIUrl":"10.5114/pjp.2024.136025","url":null,"abstract":"C1q/TNF-related protein-9 (CTRP9) has been reported to play roles in several types of retinal diseases. However, the role and the potential mechanism of CTRP9 in glaucoma are still incompletely understood. The expression of CTRP9 in OGD/R-induced retinal ganglion cells (RGCs) was detected by quantitative real-time polymerase chain reaction and western blot assay. Cell proliferation was identified by cell counting Kit-8 assay. Flow cytometry, enzyme-linked immunosorbent assay and western blot assay were performed to assess cell apoptosis. Unfolded protein response (UPR), endoplasmic reticulum (ER) stress and the AMPK pathway were evaluated by western blot assay. The data showed that the expression of CTRP9 was significantly downregulated in OGD/R-induced 661W cells. OGD/R treatment reduced cell viability, promoted cell apoptosis and activated the UPR and ER stress. The overexpression of CTRP9 reversed the effects of OGD/R on 661W cell viability, apoptosis, the UPR and ER stress, as well as the AMPK pathway. However, Compound C, an inhibitor of AMPK signaling, reversed the protection of CTRP9 overexpression against injury from OGD/R in 661W cells. In summary, the results revealed that CTRP9 abated the apoptosis and UPR of OGD/R-induced RGCs by regulating the AMPK pathway, which may provide a promising target for the treatment of glaucoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases. 激素受体阳性、人类表皮生长因子受体 2 阴性、腋窝结节病负担低的早期乳腺癌患者 Ki-67 增殖指数与肿瘤型 DX 复发评分的相关性 - 137 例病例回顾。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135859

Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili

{"title":"Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases.","authors":"Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili","doi":"10.5114/pjp.2024.135859","DOIUrl":"10.5114/pjp.2024.135859","url":null,"abstract":"The use of chemotherapy in breast cancer management has significantly contributed to the decrease in its mortality. Currently, the prognosis is determined by molecular biomarkers, such as oestrogen receptors, and human epidermal growth factor receptor 2. However, the increasing use of advanced molecular technologies, including oncotype DX recurrence score (ODX-RS), has provided the ability to estimate the risk of recurrence. Research has demonstrated that the ODX-RS helps to predict recurrence risk and the potential benefit of chemotherapy in breast cancer. As a result, it can assist clinicians in making decisions regarding using the chemotherapy. The goal of work is to explore the correlation between the ODX-RS and Ki-67 proliferative index (Ki-67-PI). This study included 137 patients with oestrogen positive, human epidermal growth factor receptor 2-negative early breast cancer, and had non- or early axillary disease. Patients with low Ki-67-PI were as follows: low ODX-RS in 17%, intermediate ODX-RS in 80%, and high ODX-RS in 2%. In the high Ki-67-PI group: low ODX-RS in 12%, intermediate ODX-RS in 48%, and high ODX-RS in 40%. In conclusion, the results show no significant correlation between the ODX-RS and Ki-67-PI (r = 0.511, p-value < 0.9).","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions. 宫颈癌前病变中 PRMT5 和 DSG2 蛋白的预后潜力。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141221

Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov

{"title":"Prognostic potential of PRMT5 and DSG2 proteins in pre-malignant cervical lesions.","authors":"Bibiana Krajňáková, Desanka Výbohová, Sandra Hurta-Csizmár, Veronika Mešťanová, Marian Adamkov","doi":"10.5114/pjp.2024.141221","DOIUrl":"https://doi.org/10.5114/pjp.2024.141221","url":null,"abstract":"Precancerous cervical lesions are metaplastic alterations of epithelial cells of the cervix, eventually developing into cervical cancer. Despite primary and secondary prevention, the burden of cervical cancer remains high globally. Protein arginine methyltransferases (PRMT) represent post-translational modifications that interact with multiple signalling pathways, playing a role in epithelial-mesenchymal transition. In complex with desmoglein-2 (DSG2), a cell adhesion protein, both participate in the progression of dysplastic changes with potential malignant development. The presented study was performed on archival paraffin-embedded blocks from adult women. The studied samples were categorised into low-grade and high-grade intraepithelial lesions. Immunohistochemical analysis was used to observe subcellular localisation, immunoreaction intensity, and percentage of PRMT5- and DSG2-expressing cells, followed by statistical analysis. Preliminary results identified statistically significant differences between the expression and subcellular localisation of proteins in question in low-grade and high-grade squamous intraepithelial lesions. The primary goal of the presented study is to perceive the involvement of PRMT5 and DSG2 in the initiation and progression of cervical lesions. Our observations indicate the potential of the assessed proteins as prognostic markers. However, further studies of PRMT5 and DSG2 are required to provide greater insight into cervical carcinogenesis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019325","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The role of the CLL-1 protein in disease monitoring in patients diagnosed with acute myeloid leukaemia and myelodysplastic syndrome. CLL-1 蛋白在急性髓性白血病和骨髓增生异常综合征患者疾病监测中的作用。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143228

Ewa Dobak, Sylwia Romana Jankowska-Szabłowska, Renata Guzicka-Kazimierczak, Paulina Poter, Elżbieta Urasińska, Katarzyna Karpińska-Łukaszewicz

引用次数: 0

Microinvasive lobular carcinoma arising in a benign phyllodes tumour - a short report and brief review of the literature. 良性蝶形花瘤中出现的微小浸润性小叶癌--简短报告和文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142085

Thomas Butters, Lynsey Williams, Charanjit Kaur, Zoltan Szollosi

引用次数: 0

Sebaceous vs. lipid-rich carcinoma of the breast. Clinicopathological characteristics and correlations - a report of two cases and a literature review. 乳腺皮脂癌与富脂癌。临床病理特征及相关性--两例病例报告及文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143114

Nikola Živković, Ana Cvetanović, Miloš Kostić, Maja Jovičić Milentijević, Miodrag Djordjević, Tijana Denčić, Julija Cvetković, Mirjana Ćuk

引用次数: 0

Expression of p16 protein in breast cancer. 乳腺癌中 p16 蛋白的表达。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136028

Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi

{"title":"Expression of p16 protein in breast cancer.","authors":"Abdulhadi Samman, Mehenaz Hanbazazh, Saad Samargandy, Jaudah Al-Maghrabi","doi":"10.5114/pjp.2024.136028","DOIUrl":"10.5114/pjp.2024.136028","url":null,"abstract":"Breast cancer is the most common cancer and a leading cause of death in women in Saudi Arabia. P16 is a tumour suppressor gene that plays a crucial role in regulating cell cycle. Several studies have investigated the significance of p16 expression in various cancer types. However, the significance of p16 in breast cancer remains controversial and insufficiently studied. The present study aims to examine the association between p16 expression and clinicopathological factors in breast cancer using immunohistochemistry staining. The study utilised 475 prospectively collected tissue samples from 475 women with breast cancer in Saudi Arabia. Nuclear and cytoplasmic immunohistochemical staining of p16 was observed in 338 (71%) of the cases and showed significant direct associations with adverse tumour features, including high tumour grade (p < 0.0001), negative oestrogen receptor/progesterone receptor status (p < 0.001), and lymph node metastasis (p = 0.02). Our study revealed a significant association between p16 protein expression and the established negative prognostic parameters in breast carcinoma including tumour grade, lymph node metastasis, and oestrogen receptor and progesterone receptor status.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer. 下调的lncRNA LINC00908与胃癌的不良预后和恶性程度增加有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141283

Chunyue Zhang, Yanmin Zhang, Yan Wang

{"title":"Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer.","authors":"Chunyue Zhang, Yanmin Zhang, Yan Wang","doi":"10.5114/pjp.2024.141283","DOIUrl":"https://doi.org/10.5114/pjp.2024.141283","url":null,"abstract":"Long non-coding RNA LINC00908 is a functional biomarker in regulating tumour progression. Its dysregulation in gastric cancer implies its potential functional role. Few studies have noted the functional role of LINC00908 in gastric cancer. The potential of LINC00908 to serve as a biomarker in gastric cancer was evaluated. A total of 113 paired gastric cancer tissues and normal tissues were collected from patients with gastric cancer. LINC00908 levels were evaluated by polymerase chain reaction, and its significance in disease progression and patients' prognosis was assessed. In vitro, the function of LINC00908 in tumour-related cellular processes was evaluated with CCK8 and Transwell assay. Significant downregulation of LINC00908 was observed in gastric cancer and was negatively associated with disease development and overall survival of patients. LINC00908 showed significant inhibitory effects on the proliferation, migration, and invasion of gastric cancer cells. Additionally, miR-627-3p was sponged by LINC00908 and therefore mediated the function of LINC00908 in gastric cancer cells. LINC00908 functioned as a prognostic biomarker and tumour suppressor of gastric cancer, providing a therapeutic target for gastric cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria. 阿尔及利亚西部乳腺癌患者中 IGF-1 (rs7136446) 和 IL-6 (rs1800795) 多态性的特征。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143230

Fatima Zohraa Boussouf, Asmahane Medjdoub, Zineb Tahari, Moussa Messatfa, Noria Bouras, Chahinez Amira Dahmani, Sonia Amal Seddiki, Tewfik Sahraoui

{"title":"Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria.","authors":"Fatima Zohraa Boussouf, Asmahane Medjdoub, Zineb Tahari, Moussa Messatfa, Noria Bouras, Chahinez Amira Dahmani, Sonia Amal Seddiki, Tewfik Sahraoui","doi":"10.5114/pjp.2024.143230","DOIUrl":"10.5114/pjp.2024.143230","url":null,"abstract":"Breast cancer (BC) is the most frequently diagnosed cancer among women worldwide, including Algeria. Certain single nucleotide polymorphisms (SNPs) have been linked to higher risk of BC. Several studies have been performed on the insulin- like growth factor 1 (IGF-1) T > C (rs7136446) and the interleukin 6 (IL-6) 174 G > C (rs1800795) SNPs to explore their role in BC. The aim of this study is to investigate the association between the 2 SNPs, IL-6 (rs1800795) and IGF-1 (rs7136446) with BC in the population of western Algeria. This study was carried out for the first time among the Algerian population. This case-control study included 109 BC patients and 112 healthy controls. Genomic DNA was extracted from peripheral blood samples. DNA concentration was determined using the Qubit 2.0 fluorometer. The genotyping of selected SNPs was performed by real-time polymerase chain reaction followed by statistical analysis to assess genotypic frequencies and genetic association with BC. The results showed that IGF-1 rs7136446 was positively associated with BC (p = 0.00001) and that the distribution of genotype frequencies of rs7136446 showed a statistically difference between human epidermal growth factor receptor 2 (HER-2) positive and HER-2 negative BC (p = 0.04), but this association did not last after the correction of Bonferroni. No association was found in genotype distribution of the IL-6 (rs1800795) among controls and BC patients or with clinicopathological parameters including HER-2 status in BC (p > 0.05). In summary, our findings indicate that IGF-1 rs7136446 is associated with BC in our population of western Algeria.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E-cadherin/CD34 double immunostaining in placental diagnosis. 胎盘诊断中的 E-cadherin/CD34双重免疫染色。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142733

Jerzy Stanek

{"title":"E-cadherin/CD34 double immunostaining in placental diagnosis.","authors":"Jerzy Stanek","doi":"10.5114/pjp.2024.142733","DOIUrl":"10.5114/pjp.2024.142733","url":null,"abstract":"Placental foetal vascular malperfusion (FVM) may be responsible for complicated foetal or neonatal condition. By highlighting endothelial fragmentation, the double E-cadherin/CD34 immunostain highlights distal villous endothelial fragmentation of recent FVM not seen on haematoxylin-eosin stained sections. We routinely perform the stain on a grossly unremarkable placental sections of placentas predominantly from pregnancies with mass-forming foetal anomalies and umbilical cord complications. The stain can upgrade the FVM and/or reveal its temporal heterogeneity, both useful in establishing the cause of foetal death or poor neonatal condition. It also highlights the basement membranes of syncytiotrophoblastic cells which, in conjunction with endothelial staining, is helpful in the diagnosis of widening thereof in distal villous hypomaturity. It can distinguish mineralised stem occluding thrombi from mineralised trophoblastic pseudo inclusions - the former outlined by CD34, the latter by E-cadherin - thus helping to differentiate FVM from placental aneuploidies. The E-cadherin component helps in the diagnosis of trophoblastic lesions of shallow placental implantation featuring an increased number of extravillous trophoblasts in placental membranes and chorionic disc. Therefore, the double immunostain is helpful in histological diagnosis of placental lesions and patterns of injury.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0