Polish Journal of Pathology最新文献_第2页

Invasive breast papillary carcinoma with mucinous stroma. 浸润性乳腺乳头状癌伴粘液间质。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156138

Joško Bezić, Toni Čeprnja

引用次数: 0

miR-21 regulates LPS-induced apoptosis and inflammatory injury in rat cardiomyocytes by targeting PLD1 and STAT3. miR-21通过靶向PLD1和STAT3调控lps诱导的大鼠心肌细胞凋亡和炎症损伤。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153974

Rui Chen, Wei Xiong, Ruiying Liu, Sai Wan, Tao Huang, Jiajing Ai, Lingjing Ye, Qingping He

{"title":"miR-21 regulates LPS-induced apoptosis and inflammatory injury in rat cardiomyocytes by targeting PLD1 and STAT3.","authors":"Rui Chen, Wei Xiong, Ruiying Liu, Sai Wan, Tao Huang, Jiajing Ai, Lingjing Ye, Qingping He","doi":"10.5114/pjp.2025.153974","DOIUrl":"https://doi.org/10.5114/pjp.2025.153974","url":null,"abstract":"This study aims to elucidate the role and molecular mechanism of microRNA-21 (miR-21) in LPS-induced inflammatory injury in H9c2 cardiomyocytes. H9c2 cardiomyocytes were treated with lipopolysaccharide (LPS) to establish an in vitro model. The expression of miR-21 was quantified using RT-qPCR, while protein levels were assessed via Western blot analysis. The impact of miR-21 on inflamma-tory response, cell proliferation, and apoptosis in LPS-treated H9c2 cells was evalu-ated using ELISA, CCK-8/EdU assays, and flow cytometry. TargetScan predictions and dual-luciferase reporter assays were employed to identify potential miR-21 tar-gets. The regulatory effects of miR-21 on inflammation, proliferation, and apop-tosis in cells were further examined following transfection with phospholipase D1 (PLD1) overexpression constructs or signal transducer and activator of transcription 3 (STAT3) activation. The expression levels of miR-21, PLD1, and p-STAT3 were significantly elevated in LPS-treated H9c2 cells. Knockdown of miR-21 markedly inhibited the LPS-induced inflammatory response, enhanced cell proliferation, and reduced apoptosis in H9c2 cells. PLD1 and STAT3 were confirmed as direct targets of miR-21. Overexpression of PLD1 or activation of STAT3 significantly reversed the protective effects of miR-21 downregulation in LPS-treated H9c2 cells. Downregu-lation of miR-21 protects cardiomyocytes against LPS-induced inflammatory injury and apoptosis by inhibiting PLD1 expression and STAT3 phosphorylation.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"131-140"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards standardized categorization of serous effusions: application of the International System for Reporting Serous Fluid Cytopathology diagnostic framework with a special emphasis on borderline ovarian tumors. 浆液积液的标准化分类：应用国际系统报告浆液细胞病理学诊断框架，特别强调交界性卵巢肿瘤。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159329

Suguna Badiginchala, Sindhuja Gv, Rukmangadha Nandyala, Aruna Kumari Prayaga

{"title":"Towards standardized categorization of serous effusions: application of the International System for Reporting Serous Fluid Cytopathology diagnostic framework with a special emphasis on borderline ovarian tumors.","authors":"Suguna Badiginchala, Sindhuja Gv, Rukmangadha Nandyala, Aruna Kumari Prayaga","doi":"10.5114/pjp.2025.159329","DOIUrl":"https://doi.org/10.5114/pjp.2025.159329","url":null,"abstract":"Borderline ovarian tumors (BOT) in effusion cytology are rare, and to date only limited literature has existed. The current study used the International System for Reporting Fluid Cytopathology (ISRSFC) criteria to reclassify 321 effusion cytology cases from 302 patients, analyzed diagnostic performance among different positive groups and evaluated the risk of malignancy (ROM). A total of 14 BOTs were included, majority of them were mucinous borderline and these tumors were reclassified into atypia of uncertain significance (AUS) and suspicious for malignancy (SFM) intermediate categories. According to ISRSFC criteria, effusion cases were categorized into five different groups: non-diagnostic (ND), negative for malignancy, AUS, SFM and malignant. The corresponding overall ROMs were 23.3%, 19.4%, 44%, 87.8% and 100%. Morphologically, mucinous BOTs in effusions presented mostly as acellular mucin, while serous BOTs exhibited mesothelial cells with few atypical cells showing mild to moderate nuclear atypia. These findings highlight the diagnostic difficulty of BOTs in effusion cytology and the need to apply ISRSFC criteria especially for AUS and SFM grey zone categories for standard reporting and clinical guidance.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"329-337"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hardware and quality-control factors influencing digital pathology reproducibility. 影响数字病理再现性的硬件和质量控制因素。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159327

Yan Li, Tiantian Li

{"title":"Hardware and quality-control factors influencing digital pathology reproducibility.","authors":"Yan Li, Tiantian Li","doi":"10.5114/pjp.2025.159327","DOIUrl":"https://doi.org/10.5114/pjp.2025.159327","url":null,"abstract":"Digital pathology (DP) converts glass slides into whole-slide images (WSI), enabling quantitative and remote diagnostics. This systematized review examines how hardware and instrumentation (HI) form the technical basis for reliable DP. Evidence from WSI validation and engineering studies shows that section-thickness variation, staining consistency, and scanner calibration are major determinants of diagnostic reproducibility. Comparative studies consistently show that even small mechanical or optical deviations - such as micron-level thickness variation or illumination shifts - produce measurable image differences that affect both inter-site diagnostic concordance and artificial intelligence model stability. We propose a unified framework linking HI parameters to quality-control (QC) indicators and regulatory requirements, including device documentation, software validation, and metadata traceability. Integrating structured metadata from tissue processing through WSI acquisition supports reproducible, auditable, and clinically compliant DP workflows. By connecting validated WSI practice, QC mechanisms, and HI-centered standardization, this review emphasizes that reliable DP depends not only on computational methods but also on precise, well-regulated, and interoperable engineering systems.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"275-283"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic challenge of primary colonic poorly cohesive adenocarcinoma exhibiting gastric‑type immunohistochemistry profile and focal signet‑ring differentiation. 显示胃型免疫组织化学特征和局灶印戒分化的原发性结肠低粘连腺癌的诊断挑战。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159280

Gizem Issın, Merve Tok, Diren Vuslat Çağatay, Oruç Numan Gökçe, Faruk Önder Aytekin

{"title":"Diagnostic challenge of primary colonic poorly cohesive adenocarcinoma exhibiting gastric‑type immunohistochemistry profile and focal signet‑ring differentiation.","authors":"Gizem Issın, Merve Tok, Diren Vuslat Çağatay, Oruç Numan Gökçe, Faruk Önder Aytekin","doi":"10.5114/pjp.2025.159280","DOIUrl":"https://doi.org/10.5114/pjp.2025.159280","url":null,"abstract":"Colorectal adenocarcinoma infrequently exhibits a diffuse, poorly cohesive architecture and a gastric-type immunophenotype, closely resembling diffuse-type gastric carcinoma and posing a formidable diagnostic challenge. This report describes a 65-year-old woman who presented with non-specific abdominal discomfort and was found by colonoscopy to have a 6.5 cm ulceroinfiltrative lesion in the hepatic flexure. Histologically, the tumor comprised discohesive cells with focal signet-ring morphology, accompanied by extensive lymphovascular and perineural invasion, necrosis, and a marked peritumoral lymphoid response. Immunohistochemical analysis demonstrated a gastric-type profile (CK7+, CK20-, SATB2-, CDX2 weak/focal, MUC5AC+, MUC2-), whereas panels excluding breast, urothelial, Müllerian, neuroendocrine, and hematolymphoid differentiation were uniformly negative. Comprehensive imaging and endoscopic evaluation excluded an extra-colonic primary malignancy/carcinoma. Mismatch repair testing revealed loss of MLH1/PMS2 expression with preserved MSH2/MSH6. This case underscores that, in such uncommon colorectal variants, reliance on immunophenotype alone may mislead, and that definitive diagnosis necessitates integrated clinicopathologic correlation, exhaustive sampling, and exclusion of alternative primaries/primary malignancies/carcinomas to confirm a primary colonic poorly cohesive adenocarcinoma with focal signet-ring differentiation.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"348-354"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

High mobility group box 1 attenuates aortic stenosis by modulating macrophages to reduce valvular calcification. 高迁移率组1通过调节巨噬细胞减少瓣膜钙化来减轻主动脉狭窄。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153975

Dong Zhao, Yun Zhao, Li-Na Luan

{"title":"High mobility group box 1 attenuates aortic stenosis by modulating macrophages to reduce valvular calcification.","authors":"Dong Zhao, Yun Zhao, Li-Na Luan","doi":"10.5114/pjp.2025.153975","DOIUrl":"https://doi.org/10.5114/pjp.2025.153975","url":null,"abstract":"Our previous study demonstrated that HMGB1 may suppress M1 macrophage polarisation and mitigate the progression of calcific aortic valve disease (CAVD). However, the role of HMGB1 in regulating macrophage-mediated valvular calcifi-cation remains to be further explored. Serum samples from healthy individuals and CAVD patients with varying severity were collected and analysed by ELISA. Immunofluorescence staining of human heart tissue arrays assessed macrophage infiltration in calcified valves. A macro-phage-aortic valve interstitial cell (haVIC) co-culture system was used to examine the effects of reHMGB1-treated macrophages. RUNX2 and osteopontin mRNA expression were measured by RT-qPCR, and alkaline phosphatase (ALP) staining was performed to evaluate calcification. HMGB1 levels were significantly reduced in severe CAVD patients than controls. Immunofluorescence staining revealed increased CD68 expression in calcified valve samples, indicating macrophage infiltration. In the macrophage-haVIC co-culture system, macrophages pretreated with reHMGB1 led to reduced RUNX2 mRNA expression and lower ALP activity in haVICs, suggesting a potential inhibitory effect of HMGB1 on valvular calcification. HMGB1 may have the potential to suppress inflammation and mitigate aortic valve calcification, making it a promising therapeutic target for preventing the progression of aortic stenosis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"141-150"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXC1 expression profile in invasive breast carcinomas and its relationship with prognostic parameters. 浸润性乳腺癌中FOXC1表达谱及其与预后参数的关系

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153969

Özge Bozkurt Öztürk, Remzi Arslan

{"title":"FOXC1 expression profile in invasive breast carcinomas and its relationship with prognostic parameters.","authors":"Özge Bozkurt Öztürk, Remzi Arslan","doi":"10.5114/pjp.2025.153969","DOIUrl":"https://doi.org/10.5114/pjp.2025.153969","url":null,"abstract":"This study aimed to investigate the expression profile of FOXC1 in molecular sub-types of invasive breast cancer. Additionally, it sought to explore the association between FOXC1 expression and clinicopathological prognostic parameters to eval-uate its potential diagnostic and therapeutic implications. A total of 122 invasive breast carcinoma cases from excision specimens were an-alysed. Immunohistochemical staining for FOXC1 was performed, with nuclear expression > 1% considered positive. The correlation between FOXC1 expression, molecular subtypes, and prognostic parameters was examined. A significant negative correlation was found between FOXC1 expression and ER, PR, and HER2 in 122 cases, while FOXC1 expression was notably higher in the triple-negative breast cancer (TNBC) subtype. Additionally, FOXC1 expression showed a significant positive correlation with nuclear grade, histological grade, Ki67 index, and prognostic stage. FOXC1 expression was found to be higher in TNBCs compared to other molecular subtypes, and FOXC1 expression was negatively correlated with hormone recep-tors and HER2. On the other hand, FOXC1 was significantly associated with high proliferation index, high-grade tumour, and prognostic stage. These findings sug-gest that high FOXC1 expression may indicate aggressive behaviour and may be a predictive marker for poor prognosis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"79-86"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does SOX-2 expression have a prognostic value in triple-negative breast cancer? SOX-2表达在三阴性乳腺癌中有预后价值吗？

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149426

Ismail Guzelis, Betul Bolat Kucukzeybek, Merve Gursoy, Yeliz Yilmaz, Yuksel Kucukzeybek

{"title":"Does SOX-2 expression have a prognostic value in triple-negative breast cancer?","authors":"Ismail Guzelis, Betul Bolat Kucukzeybek, Merve Gursoy, Yeliz Yilmaz, Yuksel Kucukzeybek","doi":"10.5114/pjp.2025.149426","DOIUrl":"https://doi.org/10.5114/pjp.2025.149426","url":null,"abstract":"Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. At the cell of origin level, cancer stem cells (CSC) are the tumour initiators in breast cancer. SRY-box transcription factor 2 (SOX-2) is a CSC marker that plays a role in tumourigenesis. The objective of this study was to evaluate the association of SOX-2 expression with histopathological parameters and clinical outcomes in TNBC patients. The study included 95 TNBC cases. An in vitro diagnostic SOX-2 antibody was applied to the tumoural slides in a validated automated stainer. The expression of SOX-2 was defined as a SOX-2 H-score ≥ 1. The expression of SOX-2 was observed in 29 cases (30.5%). At a median follow-up of 76 months, SOX-2 expression was not associated with overall or disease-free survival. R-based statistical analysis determined a SOX-2 H-score cut-off of 2. Although the overall and disease-free survival rates of cases with an H-score ≥ 3 were lower than the others, the differences were not statistically significant. The percentage of SOX-2 staining is typically low, as only 1% of tumour cells exhibit CSC characteristics. In conclusion, the prognostic significance of SOX-2 could become clear in a larger group of TNBC patients using standardized methodologies.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"1-9"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

TROP-2 expression in triple-negative and human epidermal growth factor receptor 2 enriched breast cancers and its relationship with clinicopathologic parameters. TROP-2在三阴性和人表皮生长因子受体2富集乳腺癌中的表达及其与临床病理参数的关系

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156555

Hale Kivrak, Duygu Simsek, Egemen Akincioglu, Ilke Seçinti, Gül Keskin, Ismail Erturk, Murat Demiriz

{"title":"TROP-2 expression in triple-negative and human epidermal growth factor receptor 2 enriched breast cancers and its relationship with clinicopathologic parameters.","authors":"Hale Kivrak, Duygu Simsek, Egemen Akincioglu, Ilke Seçinti, Gül Keskin, Ismail Erturk, Murat Demiriz","doi":"10.5114/pjp.2025.156555","DOIUrl":"https://doi.org/10.5114/pjp.2025.156555","url":null,"abstract":"TROP-2 is a transmembrane calcium signaling molecule that is detected at a high rate (63%) in breast cancers. There are conflicting data on its relationship with subtypes, clinical, and pathological data in breast cancer. TROP-2 expression levels were evaluated by immunohistochemistry, and the H-score method was used to analyze the data. This evaluation was conducted on a total of 79 patients diagnosed with triple-negative breast cancer (TNBC) and human epidermal growth factor receptor 2 (HER2) positive (HER2-E) breast cancer. The study also investigated the relationship between TROP-2 expression levels and the clinical-pathological features observed in patients diagnosed with TNBC and HER2-E. A total of 62 TNBC (78.5%) and 17 HER2-E (21.5%) cases were analyzed in the study. The presence of high TROP-2 (H-score > 100) was detected in 87% of the TNBC group and 94% of the HER2-E group. Among the pathological parameters, only low H-score values were found to have a statistically significant correlation with mucinous morphology; however, no significant correlation was found with other pathological parameters, including CerbB2 expression status. No significant relationship was found between H-score and clinical parameters. Furthermore, TROP-2 expression in HER2-E cancers is notably elevated. Further studies with larger series are required to clarify expression rates in mucinous tumors.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 3","pages":"248-256"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memoriam: Paweł Piotr Liberski.

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158045

Beata Sikorska, Radzisław Kordek

引用次数: 0