Polish Journal of Pathology最新文献_第2页

Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer. 下调的lncRNA LINC00908与胃癌的不良预后和恶性程度增加有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141283

Chunyue Zhang, Yanmin Zhang, Yan Wang

{"title":"Downregulated lncRNA LINC00908 correlates with a poor prognosis and increasing malignancy of gastric cancer.","authors":"Chunyue Zhang, Yanmin Zhang, Yan Wang","doi":"10.5114/pjp.2024.141283","DOIUrl":"https://doi.org/10.5114/pjp.2024.141283","url":null,"abstract":"Long non-coding RNA LINC00908 is a functional biomarker in regulating tumour progression. Its dysregulation in gastric cancer implies its potential functional role. Few studies have noted the functional role of LINC00908 in gastric cancer. The potential of LINC00908 to serve as a biomarker in gastric cancer was evaluated. A total of 113 paired gastric cancer tissues and normal tissues were collected from patients with gastric cancer. LINC00908 levels were evaluated by polymerase chain reaction, and its significance in disease progression and patients' prognosis was assessed. In vitro, the function of LINC00908 in tumour-related cellular processes was evaluated with CCK8 and Transwell assay. Significant downregulation of LINC00908 was observed in gastric cancer and was negatively associated with disease development and overall survival of patients. LINC00908 showed significant inhibitory effects on the proliferation, migration, and invasion of gastric cancer cells. Additionally, miR-627-3p was sponged by LINC00908 and therefore mediated the function of LINC00908 in gastric cancer cells. LINC00908 functioned as a prognostic biomarker and tumour suppressor of gastric cancer, providing a therapeutic target for gastric cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"157-165"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019320","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumour budding in gallbladder carcinoma. 胆囊癌的肿瘤出芽。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135860

Luisa F Rivera-Moncada, Leonardo S Lino-Silva

引用次数: 0

Deciphering signet ring cells in the bone marrow of a child - a rare case report with review of literature. 解密儿童骨髓中的印戒细胞--罕见病例报告及文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142666

Mili Jain, Anurag Singh, Preeti Agarwal, Nishant Verma

引用次数: 0

Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel. 预后不良，结肠腺癌的免疫浸润与钙激活氯通道的低表达水平有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141232

Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu

{"title":"Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel.","authors":"Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu","doi":"10.5114/pjp.2024.141232","DOIUrl":"https://doi.org/10.5114/pjp.2024.141232","url":null,"abstract":"The calcium-activated chloride channel (CLCA4) in colon adenocarcinoma (COAD) and immunological infiltration have not been extensively studied. This work thoroughly employed several datasets to assess the expression, prognosis, and association between immune infiltration and clinicopathological characteristics of CLCA4 in cancer, as well as look into potential signalling pathways. The human protein atlas (HPA), TIMER, UALCAN, TISIDB, GSCA, SangerBox, GeneMANIA, and LinkedOmics were among the datasets that were used. The findings demonstrated that, in comparison to normal tissues, COAD tissues had lower levels of CLCA4 expression. The prognosis was worse for those whose levels of CLCA4 expression were lower. For validation, immunohistochemistry (HPA) was used. Positive correlations between CLCA4 mRNA expression and its copy number variation (CNV) were observed, and CLCA4 CNV was linked to immunological infiltration. Subsequent investigation demonstrated the association between immune cell markers, immune checkpoint genes, and immunological infiltration with CLCA4. The overall survival and disease-free survival of M0 patients were considerably better than those of M1 patients, and the groups with tumour stages M0 and M1 had notably different levels of CLCA4 expression. Its substantial enrichment in ion channel activity, transmembrane transporter activity, digestion, and other biological processes was revealed by gene ontology analysis. Oxidative phosphorylation, pancreatic secretion, Parkinson's and Alzheimer's diseases, renin secretion, and other signalling pathways were the primary associations found for CLCA4. It is evident that the immunological microenvironment and functions like ion transport, metabolism, and intestinal digestion are all impacted by CLCA4 expression.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"138-152"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of PIK3CA somatic mutations with clinicopathological parameters in breast cancer.

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.145702

Gizem Teoman, Zeynep Turkmen Usta, Zeynep Sagnak Yilmaz, Sevdegul Aydin Mungan, Ismail Saygin

{"title":"Association of PIK3CA somatic mutations with clinicopathological parameters in breast cancer.","authors":"Gizem Teoman, Zeynep Turkmen Usta, Zeynep Sagnak Yilmaz, Sevdegul Aydin Mungan, Ismail Saygin","doi":"10.5114/pjp.2024.145702","DOIUrl":"https://doi.org/10.5114/pjp.2024.145702","url":null,"abstract":"The enzyme phosphatidylinositide-3-kinase (PI3K) regulates cellular proliferation and apoptosis. Somatic mutations in the PIK3CA gene can accelerate these processes and significantly contribute to the development and progression of breast cancer. This study aimed to ascertain the PIK3CA gene mutations in breast cancer patients and investigate their correlation with certain clinicopathological characteristics. We conducted a mutational investigation of the PIK3CA gene using next-generation sequencing (NGS) in a sample of 100 cases of primary breast cancer. We investigated the associations between PIK3CA mutations and clinicopathological characteristics. Our analysis revealed a mutation rate of 45% in the PIK3CA gene. The mutation frequencies for the three hotspot sites were 33.3% for E545K in exon 10, 26.7% for H1047R in exon 20, and 6.7% for E542K in exon 10. Of the 45 individuals with tumors carrying the PIK3CA mutation, 41 (91.2%) had only one mutation, while 4 (8.8%) had two. Pathogenic PIK3CA mutations were significantly correlated with tumor size ( p = 0.015) and tumor location ( p = 0.017). Our study results demonstrated a significant association between tumor size, location, and presence of the PIK3CA mutation. We must validate these data in larger sample sizes.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 4","pages":"268-273"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells.

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.146501

Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong

{"title":"Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells.","authors":"Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong","doi":"10.5114/pjp.2024.146501","DOIUrl":"https://doi.org/10.5114/pjp.2024.146501","url":null,"abstract":"Fat mass and obesity-associated protein (FTO) was the earliest discovered m6A RNA demethylase. Previous studies have indicated that m6A modifications significantly influence the development, progression, and prognosis of various cancers. This study aimed to explore the role of FTO overexpression in colorectal cancer development, as well as its biological functions. Expression levels of FTO mRNA and protein in colorectal cancer and adjacent non-cancerous tissues were assessed using RT-PCR and immunohistochemistry. FTO overexpression was achieved by transiently transfecting an FTO overexpression plasmid into the HCT15 SW480 colorectal cancer cell line. The impact of this overexpression on the cells was evaluat-ed using real-time fluorescence quantitative PCR, CCK8 proliferation assays, colony formation assays, scratch healing assays, and transwell migration and invasion assays. RT-PCR and immunohistochemistry demonstrated negligible or low FTO mRNA and protein expression in adjacent non-cancerous tissues, while high expression levels were observed in cancerous tissues. FTO overexpression in the HCT15 SW480 cell line significantly increased FTO mRNA levels compared to the control group. CCK8 assays indicated that cell proliferation was significantly higher in the FTO overexpressing group than in the control group. Colony formation assays revealed an increased number of colonies in the FTO group compared to controls. Scratch healing assays showed enhanced cell migration in the FTO group relative to controls. Transwell assays demonstrated a significant increase in invasive cell numbers in the FTO group compared to controls. In conclusion, FTO is highly expressed in colorectal cancer tissues, and its overexpression promotes proliferation and migration of colorectal cancer cells, underscoring its critical oncogenic role in this cancer type.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 4","pages":"343-352"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giant low-grade myofibroblastic sarcoma of the male breast: case report and literature review.

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143232

Shiwei Xiao, Hongtao Chen, Juan Xu, Na Fang, Zirui Ke, Mancheng Yu

引用次数: 0

Tumour budding in invasive breast carcinoma of no special type - relationship with clinicopathological parameters. 无特殊类型浸润性乳腺癌的肿瘤萌发--与临床病理参数的关系。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.139272

Özben Yalçın, Gamze Kulduk

{"title":"Tumour budding in invasive breast carcinoma of no special type - relationship with clinicopathological parameters.","authors":"Özben Yalçın, Gamze Kulduk","doi":"10.5114/pjp.2024.139272","DOIUrl":"https://doi.org/10.5114/pjp.2024.139272","url":null,"abstract":"Each breast cancer is a heterogeneous tumour with different clinicopathological feature, and thus they all have different prognoses. Tumour budding (TB), considered as the first step in tumour metastasis, is the most critical factor for poor prognosis and is associated with the epithelial-mesenchymal transition (EMT). Tumour budding and its clinicopathological features in invasive breast carcinoma of no special type (NST). Patients who underwent surgery for invasive breast carcinoma (NST) between January 2018 and 2022 were retrospectively reviewed from the database, haematoxylin and eosin-stained slides were retrieved and reevaluated. The study included 200 patients. The mean number of TB was 12.8 ±9.6. The number of TB was significantly lower in patients who underwent neoadjuvant chemotherapy treatment ( p = 0.002). There was a weak positive correlation between TB count and tumour size ( r = 0.177). Triple-negative patients had significantly lower TB counts ( p = 0.001). No significant difference was observed between histological grade, nuclear grade, presence of ductal carcinoma in situ , stromal tumour-infiltrating lymphocytes, perineural invasion, lymph node metastasis, and number of TB ( p > 0.05). The number of TB was higher in oestrogen receptor positive tumours ( p = 0.015). There were more TB in patients with angiolymphatic invasion, which supports the pathophysiological relationship between tumour budding, metastasis, and EMT. Clarification of the mechanism of TB with more studies is promising in terms of treatment options.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"77-82"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019327","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fatal pulmonary bile embolism associated with acute pancreatitis - a case report and review of the literature. 与急性胰腺炎相关的致命性肺胆汁栓塞--病例报告和文献综述。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135762

Michał Knapik, Krzysztof Okoń, Magdalena Ulatowska-Białas

引用次数: 0

Retrospective analysis of negative cytology results correlated with a positive high-risk in the human papillomavirus result and subsequent results of patients over a period of three years. 回顾性分析细胞学阴性结果与人类乳头瘤病毒阳性高风险结果的相关性，以及患者三年内的后续结果。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135828

Paweł Gerard Piotrowski, Michał Teodor Jeleń

引用次数: 0