Polish Journal of Pathology最新文献_第2页

Challenging cases of Epstein-Barr virus positive lymphomas. 具有挑战性的eb病毒阳性淋巴瘤病例。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-25 DOI: 10.5114/pjp.2026.162572

Anna Porwit

{"title":"Challenging cases of Epstein-Barr virus positive lymphomas.","authors":"Anna Porwit","doi":"10.5114/pjp.2026.162572","DOIUrl":"https://doi.org/10.5114/pjp.2026.162572","url":null,"abstract":"Epstein-Barr virus (EBV)-associated lymphomas can develop in both immuno- competent and immunocompromised patients, such as those with human immunodeficiency virus (HIV) infection, other primary or acquired immunodeficiencies, or post transplantation. The current World Health Organization classification consolidated immunodeficiency/dysregulation-related lymphoproliferative disorders into three major histopathological categories: hyperplasia, lymphoproliferative disorder of varied malignant potential, and lymphoma; and reporting the histopathological category, the presence or absence of causally associated viruses (EBV, KSHV/HHV-8), and the immune deficiency/dysregulation setting is recommended. In hematopathology practice, EBV is best confirmed by EBV-encoded RNA in situ hybridization, while immunohistochemistry is used as a complementary test for the presence of EBV-related proteins. Often, additional analyses such as fluorescence in situ hybridization and polymerase chain reaction for B- and T-cell clonality have to be applied for the final classification. This review, summarizing a lecture given at the 10th Course of the Academy of Immunohistochemistry in Cracow, Poland (June 2026), presents some challenging examples of EBV-related lymphomas. Cases include EBV+ mucocutaneous ulcer with subsequent EBV+ diffuse large B-cell lymphoma, EBV+ diffuse large B-cell lymphoma with plasma cell differentiation, post therapy EBV+ Burkitt lymphoma and cutaneous NK/T-cell lymphoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 2","pages":"162-174"},"PeriodicalIF":0.8,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148425854","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

New technologies in the genomic evaluation of lymphomas. 淋巴瘤基因组评估的新技术。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-25 DOI: 10.5114/pjp.2026.162573

Magdalena Tyburczy

引用次数: 0

The role of computational pathology in predicting expression of selected immunohistochemical markers. 计算病理学在预测选定免疫组织化学标记物表达中的作用。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-25 DOI: 10.5114/pjp.2026.162578

Mateusz Miller, Martyna Przybylska, Slawomir Pakuło, Żaneta Świderska-Chadaj

{"title":"The role of computational pathology in predicting expression of selected immunohistochemical markers.","authors":"Mateusz Miller, Martyna Przybylska, Slawomir Pakuło, Żaneta Świderska-Chadaj","doi":"10.5114/pjp.2026.162578","DOIUrl":"10.5114/pjp.2026.162578","url":null,"abstract":"Immunohistochemistry (IHC) remains a cornerstone of precision oncology, providing essential diagnostic, prognostic, and predictive molecular data. However, the traditional manual assessment of IHC slides faces persistent challenges regarding interobserver variability, reproducibility, and high diagnostic workloads. To address these limitations, artificial intelligence (AI) is increasingly integrated into computational pathology workflows. This paper outlines the current landscape of AI-assisted IHC, highlighting significant trends such as automated scoring, explainable AI, virtual staining, and multiplex analysis. We explore the transition from basic image analysis to advanced deep learning architectures capable of predicting specific IHC biomarker expression directly from standard hematoxylin and eosin morphology. Furthermore, we examine the commercial ecosystem of these tools and highlight the critical pre-analytical bottlenecks that hinder widespread clinical adoption. Finally, we present a practical case study demonstrating an automated, deep learning- based pipeline for quantifying CD34-positive myeloblasts in acute myeloid leukemia and myelodysplastic syndromes. Ultimately, while AI holds vast potential to optimize turnaround times and streamline laboratory triage, its successful clinical implementation will depend on seamless integration into existing digital pathology platforms and standardization of pre-analytical variables, moving the field from fragmented algorithms to reliable, standard-of-care diagnostic tools.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 2","pages":"209-215"},"PeriodicalIF":0.8,"publicationDate":"2026-06-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148425883","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic impact of tumour budding, stromal phenotype, and CD163-positive macrophages in intestinal-type gastric adenocarcinoma. 肠型胃腺癌中肿瘤出芽、基质表型和cd163阳性巨噬细胞对预后的影响

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161702

Elif Aydın, Ömer Atmış, Hanife Seda Mavili, Mustafa Şahbazlar, Semin Ayhan

{"title":"Prognostic impact of tumour budding, stromal phenotype, and CD163-positive macrophages in intestinal-type gastric adenocarcinoma.","authors":"Elif Aydın, Ömer Atmış, Hanife Seda Mavili, Mustafa Şahbazlar, Semin Ayhan","doi":"10.5114/pjp.2026.161702","DOIUrl":"10.5114/pjp.2026.161702","url":null,"abstract":"The tumour microenvironment plays an important role in the progression of gastric adenocarcinoma, but the prognostic value of its histopathological and immunological components in intestinal-type tumours remains unclear. This retrospective study included 100 patients who underwent gastrectomy for intestinal-type gastric adenocarcinoma. Tumour budding, stromal phenotype, tumour-stroma ratio, and lymphocytic infiltration were assessed on haematoxylin-eosin-stained sections, and immunohistochemical analyses of CD68, CD163, L-caldesmon, and periostin were performed at the invasive front. Tumour budding was associated with invasion depth and tumour stage but showed no independent prognostic significance. A myxoid stromal phenotype was independently associated with improved overall survival and reduced recurrence risk. CD163-positive macrophage density exceeded CD68 density and was independently linked to better survival. L-caldesmon expression was rare and not associated with clinicopathological parameters. Periostin expression correlated with macrophage infiltration but not with survival outcomes. These findings suggest that stromal phenotype and tumour-associated macrophages, particularly CD163-positive cells, have prognostic relevance in intestinal- type gastric adenocarcinoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 1","pages":"72-83"},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457541","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Suppressing Dicer inhibits proliferation and contributes to cellular senescence, partially via a p21-associated senescence pathway. 抑制Dicer抑制增殖并促进细胞衰老，部分通过p21相关的衰老途径。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161718

Chao-Wen Cheng, Wen-Fang Fang, Yea-Mey Yang, Jiunn-Diann Lin

{"title":"Suppressing Dicer inhibits proliferation and contributes to cellular senescence, partially via a p21-associated senescence pathway.","authors":"Chao-Wen Cheng, Wen-Fang Fang, Yea-Mey Yang, Jiunn-Diann Lin","doi":"10.5114/pjp.2026.161718","DOIUrl":"10.5114/pjp.2026.161718","url":null,"abstract":"Dicer is known to engage in regulating micro (mi)RNA biosynthesis. However, the role in driving thyroid carcinogenesis is unknown. In this study, we explored the role of Dicer in the carcinogenesis of well-differentiated thyroid carcinoma (WDTC). Thyroid cancer complementary (c)DNA and tissue arrays were purchased to measure Dicer messenger (m)RNA and protein levels. Additionally, research into silencing Dicer1 in papillary thyroid cancer (PTC) (TPC-1), follicular thyroid cancer (FTC), and normal thyroid (Nthy-ori 3-1) cell lines was also applied to evaluate its effects on tumour behaviors. Dicer1 mRNA was upregulated in PTC, while the Dicer protein was overexpressed in PTC and FTC tissues. Suppressing Dicer led to inhibition of cell proliferation in all cell lines, and repression of cell-cycle progression signatures in TPC-1 cells. Furthermore, p21 expression consistently increased in response to compromising Dicer in all four cell lines. Finally, Dicer1-knockdown TPC-1 and Nthy-ori 3-1 cells demonstrated nuclear phospho-H2AX staining, reduced bromodeoxyuridine incorporation ability, and G1 phase arrest of the cell cycle. Collectively, Dicer expression is increased in WDTC clinical samples, and Dicer regulates the proliferative ability of normal and WDTC cells, at least in part, through p21-dependent modulation of a cell-senescence mechanism linked to genomic instability.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 1","pages":"25-38"},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457568","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-step next-generation sequencing of immunoglobulin heavy chain genes to detect clonality in classical Hodgkin lymphoma. 免疫球蛋白重链基因单步测序检测经典霍奇金淋巴瘤的克隆性。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161719

Mohsen Navari, Gazzola Anna, Claudia Mannu, Luigi Cimmino, Martina Lopiano, Riccardo Donelli, Pier Paolo Piccaluga

{"title":"Single-step next-generation sequencing of immunoglobulin heavy chain genes to detect clonality in classical Hodgkin lymphoma.","authors":"Mohsen Navari, Gazzola Anna, Claudia Mannu, Luigi Cimmino, Martina Lopiano, Riccardo Donelli, Pier Paolo Piccaluga","doi":"10.5114/pjp.2026.161719","DOIUrl":"10.5114/pjp.2026.161719","url":null,"abstract":"Classical Hodgkin lymphoma (cHL) diagnosis occasionally requires clonality assessment. However, the paucity of neoplastic Hodgkin and Reed-Sternberg cells among abundant reactive elements dramatically reduces the sensitivity of standard polymerase chain reaction (PCR)-based BIOMED-2/EuroClonality protocols, which fail to detect clonality in approximately one-third of cases. Next-generation sequencing (NGS)-based approaches have been proposed to overcome this limitation, but their performance in routine diagnostics for cHL remains insufficiently evaluated. We analyzed seven cHL cases and five follicular lymphoid hyperplasia (FLH) controls, all showing polyclonal patterns by standard PCR/capillary electrophoresis. DNA extracted from formalin-fixed paraffin-embedded lymph node biopsies was subjected to immunoglobulin heavy chain (IGH) gene rearrangement analysis using the commercially available LymphoTrack® IGH assay on the MiSeq platform. Next-generation sequencing identified clonal IGH rearrangements in 3 of 7 (43%) cHL cases previously undetectable by conventional methods. Clonal cases exhibited a dominant rearrangement representing > 2.5% of total reads, with the second most frequent rearrangement being less than half of the first. The remaining cHL cases and all FLH controls displayed polyclonal patterns. The LymphoTrack® IGH NGS assay demonstrated superior sensitivity over standard PCR-based protocols for detecting clonality in cHL, supporting its implementation in routine diagnostic workflows for challenging cases.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 1","pages":"39-43"},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457603","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of hypoxia-induced autophagy related markers in malignant surface epithelial ovarian tumours. 低氧诱导自噬相关标志物在恶性卵巢表面上皮性肿瘤中的预后意义。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161233

Alaa Ibrahim Mohamed Amer, Sara A Darwish, Nehal Heabah

{"title":"Prognostic significance of hypoxia-induced autophagy related markers in malignant surface epithelial ovarian tumours.","authors":"Alaa Ibrahim Mohamed Amer, Sara A Darwish, Nehal Heabah","doi":"10.5114/pjp.2026.161233","DOIUrl":"10.5114/pjp.2026.161233","url":null,"abstract":"Bcl-2/adenovirus E1B 19-kDa interacting protein 3 (BNIP3), a pro-apoptotic protein, regulates autophagy. BNIP3 is induced in hypoxic cells, being regulated by hypoxia-inducible factor 1-α (HIF1-α). The role of hypoxia-induced autophagy in surface epithelial ovarian carcinomas (EOC) is not well established. This work aimed to evaluate BNIP3 and HIF1-α immunohistochemical expression and study the relation between their expression and patients' survival in EOC patients. High BNIP3 and HIF1-α expressions were associated with a high tumuor grade (p = 0.012 and 0.013), stage III tumuors (p = 0.031 and 0.035), presence of omental deposits (p = 0.029 and 0.004), presence of capsular invasion (p = 0.010 and 0.019), and presence of vascular invasion (p = 0.033 and 0.007). High BNIP3 and HIF1-α expressions were associated with shortened overall survival (OS) (p = 0.021 and 0.029) and poor disease-free survival (DFS) (p = 0.002 and 0.005). The relation between BNIP3 and HIF1-α immunohistochemical expression was statistically significant (p < 0.001). High BNIP3 and HIF1-α expressions are related to unfavorable clinicopathological parameters in terms of higher tumuor grade, advanced tumuors, presence of omental deposits, capsular and vascular invasion, together with poor OS and DFS in malignant surface epithelial ovarian tumuor patients.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 1","pages":"12-24"},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457562","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Pathologic assessment of response to neoadjuvant therapy in melanoma - recommendations for the evaluation of post-resection specimens. 对黑色素瘤新辅助治疗反应的病理评估-切除后标本评估的建议。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.162026

Michał Wągrodzki, Irmina Maria Michalek, Monika Durzynska, Andrzej Kaziemierz Marszałek, Andrzej Mróz, Justyna Tuziak-Klym, Kamill Sokół, Anna Małgorzata Czarnecka, Hanna Koseła-Paterczyk, Piotr Rutkowski, Anna Szumera-Ciećkiewicz

引用次数: 0

The clinicopathological significance of METTL3/SERPINE2 immunoexpression in urothelial carcinoma of the urinary bladder. METTL3/SERPINE2免疫表达在膀胱尿路上皮癌中的临床病理意义。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161459

Mina Ezzat Attya, Hany Yousry Sayed

{"title":"The clinicopathological significance of METTL3/SERPINE2 immunoexpression in urothelial carcinoma of the urinary bladder.","authors":"Mina Ezzat Attya, Hany Yousry Sayed","doi":"10.5114/pjp.2026.161459","DOIUrl":"10.5114/pjp.2026.161459","url":null,"abstract":"The aim of this study was to investigate the immunohistochemical expression of METTL3 and SERPINE2 in urothelial carcinoma and to assess their individual and combined associations with clinicopathological features and possible correlation. This study included 126 cases of urothelial carcinoma. Immunohistochemical staining was used to evaluate METTL3 and SERPINE2 expression in tumour tissues. Clinico- pathological data including age, sex, bilharziasis, tumour grade and stage, lymph node status, lymphovascular invasion, soft tissue surgical margins, and tumour- infiltrating lymphocytes (TIL) were collected. Statistical analysis was performed to determine associations between marker expression and clinicopathological parameters. High nuclear METTL3 expression was significantly associated with a higher tumour grade, advanced stage, increased TIL, and lymphovascular invasion, with no significant association with age, sex, bilharziasis, nodal stage, or surgical margins. High cytoplasmic SERPINE2 expression was significantly associated with an advanced stage, lymph node metastasis, and positive soft tissue margins, while no association was found with age, sex, bilharziasis, grade, TIL, or lymphovascular invasion. Combined high METTL3/high SERPINE2 expression correlated significantly with a high grade, advanced tumour and nodal stages, low TIL, and positive surgical margins, indicating a more aggressive tumour phenotype. METTL3 and SERPINE2 are associated with adverse pathological features in urothelial carcinoma, and their combined overexpression may serve as a potential prognostic biomarker.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"77 1","pages":"53-61"},"PeriodicalIF":0.8,"publicationDate":"2026-06-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"148457570","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The hidden tumour: giant anterior mediastinal thymolipoma, an incidental finding. 隐藏肿瘤：巨大前纵隔胸腺脂肪瘤，偶然发现。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2026-06-08 DOI: 10.5114/pjp.2026.161537

Banyameen Iqbal, Mangesh Machindra Londhe, Sushama Gurwale, Pragati Gupta

引用次数: 0