Polish Journal of Pathology最新文献_第4页

Retracted: Histopathological image analysis and enhanced diagnostic accuracy explainability for oral cancer detection. 撤回：组织病理学图像分析和提高口腔癌检测的诊断准确性。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159057

V P Gladis Pushparathi, Sylaja Vallee Narayan S R, Pratheeba R S, V Naveen

引用次数: 0

Neural proximity, vascular remodeling, and perineural immune exclusion across the cervical neoplasia spectrum - a whole-slide, nerve-anchored spatial analysis of 135 cases. 神经接近、血管重构和神经周围免疫排斥贯穿宫颈肿瘤谱——135例全切片、神经锚定空间分析

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158907

Giorgi-Jemal Gogitidze, Shota Kepuladze, George Burkadze

{"title":"Neural proximity, vascular remodeling, and perineural immune exclusion across the cervical neoplasia spectrum - a whole-slide, nerve-anchored spatial analysis of 135 cases.","authors":"Giorgi-Jemal Gogitidze, Shota Kepuladze, George Burkadze","doi":"10.5114/pjp.2025.158907","DOIUrl":"10.5114/pjp.2025.158907","url":null,"abstract":"Cervical carcinogenesis unfolds within a complex tissue ecosystem. While epithelial biomarkers and HPV status inform risk, the contribution of local neural circuits and their relationship to angiogenesis and cytotoxic immunity remains insufficiently defined in cervical intraepithelial neoplasia (CIN). In a balanced, archival cohort (n = 135; 27 per group: normal, CIN1, CIN2, CIN3, squamous cell carcinoma - SCC), whole-slide images were analyzed using a pre-specified QuPath pipeline with fixed thresholds and area normalization. We created composite indices: a neuro-epithelial coupling index (NECI) integrating nerve density, caliber, PGP9.5 signal, and inverse nerve-basement membrane (BM) distance; a vascular remodeling index (VRI) integrating CD31/CD34 microvessel density, vascular endothelial growth factor, vessel caliber, and peribasement vessels. Cytotoxic access was captured by CD8 density within 0-50 µm of nerves (NACD50) NECI and VRI increased monotonically with grade (group medians, normal  SCC: NECI -1.29, -0.64, 0.00, 1.33, 3.54; VRI -1.35, -0.58, 0.00, 1.14, 2.06). Neurovascular progression index values differ between grades (-1.32, -0.64, 0.00, 1.24, 2.66). The minimum nerve-BM distance shortened stepwise. In contrast, nerve-adjacent CD8 access declined with grade: NACD50 fell (≈ 5, 8, 4, 2, 1 cells/mm²) and nerve-avoidance ratio decreased (≈ 0.9, 0.8, 0.6, 0.4, 0.3), indicating progressive perineural CD8 exclusion. Across the full histologic spectrum, neuro-epithelial proximity and vascular remodeling intensify, whereas cytotoxic access to the perineural niche declines. These nerve-anchored, spatially explicit metrics add a neural dimension to cervical carcinogenesis and nominate the 0-50 µm perineural zone as a quantifiable compartment for risk stratification and interventional trials. External validation and calibrated modeling are warranted.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"318-328"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic consistency of programmed death-ligand 1 immunohistochemistry - comparative analysis of SP142 and 22C3 clones in non-small cell lung cancer. 程序性死亡-配体1免疫组化诊断的一致性——SP142和22C3克隆在非小细胞肺癌中的比较分析。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158889

Songül Şahin, Sule Ayla

{"title":"Diagnostic consistency of programmed death-ligand 1 immunohistochemistry - comparative analysis of SP142 and 22C3 clones in non-small cell lung cancer.","authors":"Songül Şahin, Sule Ayla","doi":"10.5114/pjp.2025.158889","DOIUrl":"10.5114/pjp.2025.158889","url":null,"abstract":"Programmed death-ligand 1 (PD-L1) expression is a critical predictive biomarker for immune checkpoint inhibitor therapies in non-small cell lung cancer (NSCLC). Although various antibody clones are used for immunohistochemical (IHC) detection, differences in platforms and scoring algorithms raise concerns regarding analytical agreement at clinically relevant thresholds. This study evaluated the analytical concordance between VENTANA SP142, VENTANA 22C3, and DAKO 22C3 PD-L1 IHC assays in NSCLC, focusing on therapeutic decision-making thresholds. Non-small cell lung cancer samples from 205 patients were stained using VENTANA SP142, VENTANA 22C3, and DAKO 22C3 assays. Programmed death-ligand 1 expression was assessed in tumor cells using predefined thresholds (< 1%, 1-49%, ≥ 50%). Inter-assay agreement was evaluated using intraclass correlation coefficients (ICC). Strong analytical agreement (ICC > 0.90) was observed between SP142 and both DAKO and VENTANA 22C3 assays in the < 1% and 1-49% expression groups. Agreement decreased between VENTANA SP142 and DAKO 22C3 at the ≥ 50% threshold (ICC = 0.704). Conversely, concordance between VENTANA and DAKO 22C3 was high across all thresholds, particularly in the < 1% and ≥ 50% subgroups (ICC = 1.0 and 0.867, respectively). VENTANA and DAKO 22C3 assays demonstrate high analytical agreement. While SP142 is comparable at low expression levels, its reliability declines at higher thresholds. These findings underscore the necessity of standardized assay selection for accurate immunotherapy stratification in NSCLC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"299-306"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576245","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

miR-21 regulates LPS-induced apoptosis and inflammatory injury in rat cardiomyocytes by targeting PLD1 and STAT3. miR-21通过靶向PLD1和STAT3调控lps诱导的大鼠心肌细胞凋亡和炎症损伤。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153974

Rui Chen, Wei Xiong, Ruiying Liu, Sai Wan, Tao Huang, Jiajing Ai, Lingjing Ye, Qingping He

{"title":"miR-21 regulates LPS-induced apoptosis and inflammatory injury in rat cardiomyocytes by targeting PLD1 and STAT3.","authors":"Rui Chen, Wei Xiong, Ruiying Liu, Sai Wan, Tao Huang, Jiajing Ai, Lingjing Ye, Qingping He","doi":"10.5114/pjp.2025.153974","DOIUrl":"https://doi.org/10.5114/pjp.2025.153974","url":null,"abstract":"This study aims to elucidate the role and molecular mechanism of microRNA-21 (miR-21) in LPS-induced inflammatory injury in H9c2 cardiomyocytes. H9c2 cardiomyocytes were treated with lipopolysaccharide (LPS) to establish an in vitro model. The expression of miR-21 was quantified using RT-qPCR, while protein levels were assessed via Western blot analysis. The impact of miR-21 on inflamma-tory response, cell proliferation, and apoptosis in LPS-treated H9c2 cells was evalu-ated using ELISA, CCK-8/EdU assays, and flow cytometry. TargetScan predictions and dual-luciferase reporter assays were employed to identify potential miR-21 tar-gets. The regulatory effects of miR-21 on inflammation, proliferation, and apop-tosis in cells were further examined following transfection with phospholipase D1 (PLD1) overexpression constructs or signal transducer and activator of transcription 3 (STAT3) activation. The expression levels of miR-21, PLD1, and p-STAT3 were significantly elevated in LPS-treated H9c2 cells. Knockdown of miR-21 markedly inhibited the LPS-induced inflammatory response, enhanced cell proliferation, and reduced apoptosis in H9c2 cells. PLD1 and STAT3 were confirmed as direct targets of miR-21. Overexpression of PLD1 or activation of STAT3 significantly reversed the protective effects of miR-21 downregulation in LPS-treated H9c2 cells. Downregu-lation of miR-21 protects cardiomyocytes against LPS-induced inflammatory injury and apoptosis by inhibiting PLD1 expression and STAT3 phosphorylation.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"131-140"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114780","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Spontaneous pathological splenic rupture as a first manifestation of mantle cell lymphoma - a case report and literature review. 以套细胞淋巴瘤为首发表现的自发性病理性脾破裂1例报告并文献复习。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156558

Antoni Knera, Wojciech Ciesielski, Adam Placek, Piotr Kruszyński, Marcin Braun, Julia Sołek, Janusz Strzelczyk, Adam Durczyński, Tomasz Klimczak, Piotr Hogendorf

引用次数: 0

Invasive breast papillary carcinoma with mucinous stroma. 浸润性乳腺乳头状癌伴粘液间质。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156138

Joško Bezić, Toni Čeprnja

引用次数: 0

On interactions between fever, inflammation, and the autonomic nervous system. 发热、炎症和自主神经系统的相互作用。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149441

Beato Suwa

{"title":"On interactions between fever, inflammation, and the autonomic nervous system.","authors":"Beato Suwa","doi":"10.5114/pjp.2025.149441","DOIUrl":"10.5114/pjp.2025.149441","url":null,"abstract":"The pathologist Simon Samuel, one of the pioneers in the field of the autonomic nervous system during the 19 th century, contributed significantly to the understanding of the pathological mechanisms underlying inflammation. The remarkable advances in that field around the mid-19 th century fundamentally influenced future research of the last 170 years. In fact these findings are still connected with many novel studies on pathology today. Albert Eulenburg (1840-1917) is another scientist who contributed to the former advances in the autonomic nervous system and who closely cooperated with Samuel. Both scientists published articles and reviews in the German journal Schmidt's yearbooks edited by J. A. Winter (1816-1901). It is remarkable that Johann Ignaz Hoppe (1811-1891), an even earlier pioneer of the autonomic nervous system, also wrote articles for the same journal and obviously had influenced Samuel. Although regulations discriminating Jewish academics existed in Prussia (and later in the German Empire) until approximately 1888, Samuel was finally made assistant professor (außerordentlicher Professor) at Königsberg University (today Kaliningrad/Russian Federation) in 1874. An embarrassingly low position compared to the significance of his scientific contributions and to the implications of his work on future generations.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"47-53"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Towards standardized categorization of serous effusions: application of the International System for Reporting Serous Fluid Cytopathology diagnostic framework with a special emphasis on borderline ovarian tumors. 浆液积液的标准化分类：应用国际系统报告浆液细胞病理学诊断框架，特别强调交界性卵巢肿瘤。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159329

Suguna Badiginchala, Sindhuja Gv, Rukmangadha Nandyala, Aruna Kumari Prayaga

{"title":"Towards standardized categorization of serous effusions: application of the International System for Reporting Serous Fluid Cytopathology diagnostic framework with a special emphasis on borderline ovarian tumors.","authors":"Suguna Badiginchala, Sindhuja Gv, Rukmangadha Nandyala, Aruna Kumari Prayaga","doi":"10.5114/pjp.2025.159329","DOIUrl":"10.5114/pjp.2025.159329","url":null,"abstract":"Borderline ovarian tumors (BOT) in effusion cytology are rare, and to date only limited literature has existed. The current study used the International System for Reporting Fluid Cytopathology (ISRSFC) criteria to reclassify 321 effusion cytology cases from 302 patients, analyzed diagnostic performance among different positive groups and evaluated the risk of malignancy (ROM). A total of 14 BOTs were included, majority of them were mucinous borderline and these tumors were reclassified into atypia of uncertain significance (AUS) and suspicious for malignancy (SFM) intermediate categories. According to ISRSFC criteria, effusion cases were categorized into five different groups: non-diagnostic (ND), negative for malignancy, AUS, SFM and malignant. The corresponding overall ROMs were 23.3%, 19.4%, 44%, 87.8% and 100%. Morphologically, mucinous BOTs in effusions presented mostly as acellular mucin, while serous BOTs exhibited mesothelial cells with few atypical cells showing mild to moderate nuclear atypia. These findings highlight the diagnostic difficulty of BOTs in effusion cytology and the need to apply ISRSFC criteria especially for AUS and SFM grey zone categories for standard reporting and clinical guidance.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"329-337"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Hardware and quality-control factors influencing digital pathology reproducibility. 影响数字病理再现性的硬件和质量控制因素。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159327

Yan Li, Tiantian Li

{"title":"Hardware and quality-control factors influencing digital pathology reproducibility.","authors":"Yan Li, Tiantian Li","doi":"10.5114/pjp.2025.159327","DOIUrl":"10.5114/pjp.2025.159327","url":null,"abstract":"Digital pathology (DP) converts glass slides into whole-slide images (WSI), enabling quantitative and remote diagnostics. This systematized review examines how hardware and instrumentation (HI) form the technical basis for reliable DP. Evidence from WSI validation and engineering studies shows that section-thickness variation, staining consistency, and scanner calibration are major determinants of diagnostic reproducibility. Comparative studies consistently show that even small mechanical or optical deviations - such as micron-level thickness variation or illumination shifts - produce measurable image differences that affect both inter-site diagnostic concordance and artificial intelligence model stability. We propose a unified framework linking HI parameters to quality-control (QC) indicators and regulatory requirements, including device documentation, software validation, and metadata traceability. Integrating structured metadata from tissue processing through WSI acquisition supports reproducible, auditable, and clinically compliant DP workflows. By connecting validated WSI practice, QC mechanisms, and HI-centered standardization, this review emphasizes that reliable DP depends not only on computational methods but also on precise, well-regulated, and interoperable engineering systems.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"275-283"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576242","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Diagnostic challenge of primary colonic poorly cohesive adenocarcinoma exhibiting gastric‑type immunohistochemistry profile and focal signet‑ring differentiation. 显示胃型免疫组织化学特征和局灶印戒分化的原发性结肠低粘连腺癌的诊断挑战。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159280

Gizem Issın, Merve Tok, Diren Vuslat Çağatay, Oruç Numan Gökçe, Faruk Önder Aytekin

{"title":"Diagnostic challenge of primary colonic poorly cohesive adenocarcinoma exhibiting gastric‑type immunohistochemistry profile and focal signet‑ring differentiation.","authors":"Gizem Issın, Merve Tok, Diren Vuslat Çağatay, Oruç Numan Gökçe, Faruk Önder Aytekin","doi":"10.5114/pjp.2025.159280","DOIUrl":"10.5114/pjp.2025.159280","url":null,"abstract":"Colorectal adenocarcinoma infrequently exhibits a diffuse, poorly cohesive architecture and a gastric-type immunophenotype, closely resembling diffuse-type gastric carcinoma and posing a formidable diagnostic challenge. This report describes a 65-year-old woman who presented with non-specific abdominal discomfort and was found by colonoscopy to have a 6.5 cm ulceroinfiltrative lesion in the hepatic flexure. Histologically, the tumor comprised discohesive cells with focal signet-ring morphology, accompanied by extensive lymphovascular and perineural invasion, necrosis, and a marked peritumoral lymphoid response. Immunohistochemical analysis demonstrated a gastric-type profile (CK7+, CK20-, SATB2-, CDX2 weak/focal, MUC5AC+, MUC2-), whereas panels excluding breast, urothelial, Müllerian, neuroendocrine, and hematolymphoid differentiation were uniformly negative. Comprehensive imaging and endoscopic evaluation excluded an extra-colonic primary malignancy/carcinoma. Mismatch repair testing revealed loss of MLH1/PMS2 expression with preserved MSH2/MSH6. This case underscores that, in such uncommon colorectal variants, reliance on immunophenotype alone may mislead, and that definitive diagnosis necessitates integrated clinicopathologic correlation, exhaustive sampling, and exclusion of alternative primaries/primary malignancies/carcinomas to confirm a primary colonic poorly cohesive adenocarcinoma with focal signet-ring differentiation.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"348-354"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576269","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0