Polish Journal of Pathology最新文献_第4页

Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria. 阿尔及利亚西部乳腺癌患者中 IGF-1 (rs7136446) 和 IL-6 (rs1800795) 多态性的特征。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143230

Fatima Zohraa Boussouf, Asmahane Medjdoub, Zineb Tahari, Moussa Messatfa, Noria Bouras, Chahinez Amira Dahmani, Sonia Amal Seddiki, Tewfik Sahraoui

{"title":"Characterisation of IGF-1 (rs7136446) and IL-6 (rs1800795) polymorphisms among breast cancer patients in western Algeria.","authors":"Fatima Zohraa Boussouf, Asmahane Medjdoub, Zineb Tahari, Moussa Messatfa, Noria Bouras, Chahinez Amira Dahmani, Sonia Amal Seddiki, Tewfik Sahraoui","doi":"10.5114/pjp.2024.143230","DOIUrl":"10.5114/pjp.2024.143230","url":null,"abstract":"Breast cancer (BC) is the most frequently diagnosed cancer among women worldwide, including Algeria. Certain single nucleotide polymorphisms (SNPs) have been linked to higher risk of BC. Several studies have been performed on the insulin- like growth factor 1 (IGF-1) T > C (rs7136446) and the interleukin 6 (IL-6) 174 G > C (rs1800795) SNPs to explore their role in BC. The aim of this study is to investigate the association between the 2 SNPs, IL-6 (rs1800795) and IGF-1 (rs7136446) with BC in the population of western Algeria. This study was carried out for the first time among the Algerian population. This case-control study included 109 BC patients and 112 healthy controls. Genomic DNA was extracted from peripheral blood samples. DNA concentration was determined using the Qubit 2.0 fluorometer. The genotyping of selected SNPs was performed by real-time polymerase chain reaction followed by statistical analysis to assess genotypic frequencies and genetic association with BC. The results showed that IGF-1 rs7136446 was positively associated with BC (p = 0.00001) and that the distribution of genotype frequencies of rs7136446 showed a statistically difference between human epidermal growth factor receptor 2 (HER-2) positive and HER-2 negative BC (p = 0.04), but this association did not last after the correction of Bonferroni. No association was found in genotype distribution of the IL-6 (rs1800795) among controls and BC patients or with clinicopathological parameters including HER-2 status in BC (p > 0.05). In summary, our findings indicate that IGF-1 rs7136446 is associated with BC in our population of western Algeria.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 3","pages":"205-214"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

E-cadherin/CD34 double immunostaining in placental diagnosis. 胎盘诊断中的 E-cadherin/CD34双重免疫染色。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142733

Jerzy Stanek

{"title":"E-cadherin/CD34 double immunostaining in placental diagnosis.","authors":"Jerzy Stanek","doi":"10.5114/pjp.2024.142733","DOIUrl":"10.5114/pjp.2024.142733","url":null,"abstract":"Placental foetal vascular malperfusion (FVM) may be responsible for complicated foetal or neonatal condition. By highlighting endothelial fragmentation, the double E-cadherin/CD34 immunostain highlights distal villous endothelial fragmentation of recent FVM not seen on haematoxylin-eosin stained sections. We routinely perform the stain on a grossly unremarkable placental sections of placentas predominantly from pregnancies with mass-forming foetal anomalies and umbilical cord complications. The stain can upgrade the FVM and/or reveal its temporal heterogeneity, both useful in establishing the cause of foetal death or poor neonatal condition. It also highlights the basement membranes of syncytiotrophoblastic cells which, in conjunction with endothelial staining, is helpful in the diagnosis of widening thereof in distal villous hypomaturity. It can distinguish mineralised stem occluding thrombi from mineralised trophoblastic pseudo inclusions - the former outlined by CD34, the latter by E-cadherin - thus helping to differentiate FVM from placental aneuploidies. The E-cadherin component helps in the diagnosis of trophoblastic lesions of shallow placental implantation featuring an increased number of extravillous trophoblasts in placental membranes and chorionic disc. Therefore, the double immunostain is helpful in histological diagnosis of placental lesions and patterns of injury.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 3","pages":"171-181"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142511747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Tumour budding in gallbladder carcinoma. 胆囊癌的肿瘤出芽。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135860

Luisa F Rivera-Moncada, Leonardo S Lino-Silva

引用次数: 0

Deciphering signet ring cells in the bone marrow of a child - a rare case report with review of literature. 解密儿童骨髓中的印戒细胞--罕见病例报告及文献综述。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.142666

Mili Jain, Anurag Singh, Preeti Agarwal, Nishant Verma

引用次数: 0

Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel. 预后不良，结肠腺癌的免疫浸润与钙激活氯通道的低表达水平有关。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.141232

Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu

{"title":"Prognosis poor, immune infiltration of colon adenocarcinoma associated with low expression levels of calcium-activated chloride channel.","authors":"Xueying Zhao, Yunfan Chen, Liyuan Wang, Dongli Sui, Jin Lu","doi":"10.5114/pjp.2024.141232","DOIUrl":"https://doi.org/10.5114/pjp.2024.141232","url":null,"abstract":"The calcium-activated chloride channel (CLCA4) in colon adenocarcinoma (COAD) and immunological infiltration have not been extensively studied. This work thoroughly employed several datasets to assess the expression, prognosis, and association between immune infiltration and clinicopathological characteristics of CLCA4 in cancer, as well as look into potential signalling pathways. The human protein atlas (HPA), TIMER, UALCAN, TISIDB, GSCA, SangerBox, GeneMANIA, and LinkedOmics were among the datasets that were used. The findings demonstrated that, in comparison to normal tissues, COAD tissues had lower levels of CLCA4 expression. The prognosis was worse for those whose levels of CLCA4 expression were lower. For validation, immunohistochemistry (HPA) was used. Positive correlations between CLCA4 mRNA expression and its copy number variation (CNV) were observed, and CLCA4 CNV was linked to immunological infiltration. Subsequent investigation demonstrated the association between immune cell markers, immune checkpoint genes, and immunological infiltration with CLCA4. The overall survival and disease-free survival of M0 patients were considerably better than those of M1 patients, and the groups with tumour stages M0 and M1 had notably different levels of CLCA4 expression. Its substantial enrichment in ion channel activity, transmembrane transporter activity, digestion, and other biological processes was revealed by gene ontology analysis. Oxidative phosphorylation, pancreatic secretion, Parkinson's and Alzheimer's diseases, renin secretion, and other signalling pathways were the primary associations found for CLCA4. It is evident that the immunological microenvironment and functions like ion transport, metabolism, and intestinal digestion are all impacted by CLCA4 expression.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 2","pages":"138-152"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142019324","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Association of PIK3CA somatic mutations with clinicopathological parameters in breast cancer. 乳腺癌中PIK3CA体细胞突变与临床病理参数的关系

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.145702

Gizem Teoman, Zeynep Turkmen Usta, Zeynep Sagnak Yilmaz, Sevdegul Aydin Mungan, Ismail Saygin

{"title":"Association of PIK3CA somatic mutations with clinicopathological parameters in breast cancer.","authors":"Gizem Teoman, Zeynep Turkmen Usta, Zeynep Sagnak Yilmaz, Sevdegul Aydin Mungan, Ismail Saygin","doi":"10.5114/pjp.2024.145702","DOIUrl":"10.5114/pjp.2024.145702","url":null,"abstract":"The enzyme phosphatidylinositide-3-kinase (PI3K) regulates cellular proliferation and apoptosis. Somatic mutations in the PIK3CA gene can accelerate these processes and significantly contribute to the development and progression of breast cancer. This study aimed to ascertain the PIK3CA gene mutations in breast cancer patients and investigate their correlation with certain clinicopathological characteristics. We conducted a mutational investigation of the PIK3CA gene using next-generation sequencing (NGS) in a sample of 100 cases of primary breast cancer. We investigated the associations between PIK3CA mutations and clinicopathological characteristics. Our analysis revealed a mutation rate of 45% in the PIK3CA gene. The mutation frequencies for the three hotspot sites were 33.3% for E545K in exon 10, 26.7% for H1047R in exon 20, and 6.7% for E542K in exon 10. Of the 45 individuals with tumors carrying the PIK3CA mutation, 41 (91.2%) had only one mutation, while 4 (8.8%) had two. Pathogenic PIK3CA mutations were significantly correlated with tumor size ( p = 0.015) and tumor location ( p = 0.017). Our study results demonstrated a significant association between tumor size, location, and presence of the PIK3CA mutation. We must validate these data in larger sample sizes.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 4","pages":"268-273"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030166","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells. 结直肠癌中脂肪量和肥胖相关蛋白表达及其对结直肠癌细胞生物学行为的影响

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.146501

Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong

{"title":"Fat mass and obesity-associated protein expression in colorectal cancer and its influence on the biological behaviour of colorectal cancer cells.","authors":"Huihui Wu, Yibo Wu, Zhishan Zhang, Qinglin Chen, Tingjin Zheng, Hui Zhong","doi":"10.5114/pjp.2024.146501","DOIUrl":"10.5114/pjp.2024.146501","url":null,"abstract":"Fat mass and obesity-associated protein (FTO) was the earliest discovered m6A RNA demethylase. Previous studies have indicated that m6A modifications significantly influence the development, progression, and prognosis of various cancers. This study aimed to explore the role of FTO overexpression in colorectal cancer development, as well as its biological functions. Expression levels of FTO mRNA and protein in colorectal cancer and adjacent non-cancerous tissues were assessed using RT-PCR and immunohistochemistry. FTO overexpression was achieved by transiently transfecting an FTO overexpression plasmid into the HCT15 SW480 colorectal cancer cell line. The impact of this overexpression on the cells was evaluat-ed using real-time fluorescence quantitative PCR, CCK8 proliferation assays, colony formation assays, scratch healing assays, and transwell migration and invasion assays. RT-PCR and immunohistochemistry demonstrated negligible or low FTO mRNA and protein expression in adjacent non-cancerous tissues, while high expression levels were observed in cancerous tissues. FTO overexpression in the HCT15 SW480 cell line significantly increased FTO mRNA levels compared to the control group. CCK8 assays indicated that cell proliferation was significantly higher in the FTO overexpressing group than in the control group. Colony formation assays revealed an increased number of colonies in the FTO group compared to controls. Scratch healing assays showed enhanced cell migration in the FTO group relative to controls. Transwell assays demonstrated a significant increase in invasive cell numbers in the FTO group compared to controls. In conclusion, FTO is highly expressed in colorectal cancer tissues, and its overexpression promotes proliferation and migration of colorectal cancer cells, underscoring its critical oncogenic role in this cancer type.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 4","pages":"343-352"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143030217","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Giant low-grade myofibroblastic sarcoma of the male breast: case report and literature review. 男性乳房巨大低级别肌纤维母细胞肉瘤1例报告及文献复习。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.143232

Shiwei Xiao, Hongtao Chen, Juan Xu, Na Fang, Zirui Ke, Mancheng Yu

引用次数: 0

Fatal pulmonary bile embolism associated with acute pancreatitis - a case report and review of the literature. 与急性胰腺炎相关的致命性肺胆汁栓塞--病例报告和文献综述。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135762

Michał Knapik, Krzysztof Okoń, Magdalena Ulatowska-Białas

引用次数: 0

Retrospective analysis of negative cytology results correlated with a positive high-risk in the human papillomavirus result and subsequent results of patients over a period of three years. 回顾性分析细胞学阴性结果与人类乳头瘤病毒阳性高风险结果的相关性，以及患者三年内的后续结果。