Polish Journal of Pathology最新文献_第5页

Primary epithelioid angiosarcoma of endometrium. 原发性子宫内膜上皮样血管肉瘤。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132302

Selim Sevim, Ekin Gedik, Hilal Ozakinci, Gulsah Kaygusuz, Duygu Enneli

引用次数: 0

The Krüppel-like factor 6 represses proliferation and invasion of cutaneous malignant melanoma. kr<s:1> ppel样因子6抑制皮肤恶性黑色素瘤的增殖和侵袭。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132224

Yuan-Yuan Li, Qi-Ming Zheng, Nan Zhang, Min Zhang

{"title":"The Krüppel-like factor 6 represses proliferation and invasion of cutaneous malignant melanoma.","authors":"Yuan-Yuan Li, Qi-Ming Zheng, Nan Zhang, Min Zhang","doi":"10.5114/pjp.2023.132224","DOIUrl":"10.5114/pjp.2023.132224","url":null,"abstract":"Cutaneous carcinoma is one of the most common neoplasm tumors in the West. Its incidence rate is one of the fastest growing tumors in China. The Krüppel-like factor 6 (KLF6) is a latent tumor suppressor. Decreased KLF6 is related to the occurrence and progression of many cancers in human. Our previous studies have demonstrated that KLF6 was down-regulation in cutaneous malignant melanoma (CMM), and was significant correlated with ulcer, lymph node metastasis and clinical stage, suggesting that KLF6 loss is expected to become a biological indicator of poor prognosis in CMM patients. In this research, we would further study the features of KLF6 in the malignant progression of CMM. The expression of KLF6 was up-regulated by lentivirus infection containing KLF6, and short hairpin RNA (shRNA) was used for knockdown of KLF6 in CMM cells. Western blot, RT-qpcr, CCK8 assay, transwell migration assays, wound healing assay and flow cytometry were used to test the role of KLF6 in the CMM. We found that reduced expression of KLF6 significantly enhanced proliferation, migration and invasion. Moreover, KLF6 induced CMM cell apoptosis and G1 cycle arrest. The decreased KLF6 expression is expected to be a biological indicator of poor prognosis in CMM patients.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720246","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Assessment of the diagnostic usefulness of liquid-based cytology. The impact of modifications. 液基细胞学诊断实用性评估。修改的影响。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.134320

Andrzej Marszałek, Joanna Bakinowska, Małgorzata Grobelna, Adam Śliwiński

{"title":"Assessment of the diagnostic usefulness of liquid-based cytology. The impact of modifications.","authors":"Andrzej Marszałek, Joanna Bakinowska, Małgorzata Grobelna, Adam Śliwiński","doi":"10.5114/pjp.2023.134320","DOIUrl":"10.5114/pjp.2023.134320","url":null,"abstract":"The technique of liquid-based cytology (LBC) is of increasing diagnostic value in non-gynecological cytology. The purpose of the present study was to validate modifications to stages of the LBC process and to assess the diagnostic usefulness of the LBC technique. Between May 2021 and January 2022, a total of 484 samples were prepared from routine cytology tests carried out in the Department of Tumor Pathology of The Greater Poland Cancer Center. The material was obtained from fine-needle aspiration biopsies and fluid samples. One hundred cases were selected for the research described in the article. The slides were prepared using the Becton Dickinson Totalys SlidePrep device. Based on the research, it was concluded that predominantly the LBC technique gives better results than a conventional smear because the background, which would otherwise make it difficult to assess preparations, was eliminated. Additionally, in LBC preparations the cellularity was increased, and the cells were arranged in a monolayer system, which improves the image quality. The introduction of modifications to the LBC method facilitated the process of sample preparation, without adversely affecting the quality of the obtained material.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112001","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Clinicopathological significance of Obg-like ATPase 1 and its association with Snail in gastric cancer. 胃癌中obg样atp酶1的临床病理意义及其与蜗牛的关系。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.127265

Juan Wang, Qinghua Cao, Zhiqing Wang

{"title":"Clinicopathological significance of Obg-like ATPase 1 and its association with Snail in gastric cancer.","authors":"Juan Wang, Qinghua Cao, Zhiqing Wang","doi":"10.5114/pjp.2023.127265","DOIUrl":"https://doi.org/10.5114/pjp.2023.127265","url":null,"abstract":"Obg-like ATPase 1 (OLA1) is a member of the Obg family of P-loop NTPases and has recently been detected in several human cancer cells. However, its expression type and clinical relevance in gastric cancer remains unclear. In the present study, mRNA level of OLA1 in gastric cancer (GC) was detected in 2 datasets downloaded from the open Gene Expression Omnibus database and 30 cancer tissues. Immunohistochemistry was performed on GC and its association with Snail in 334 GC patients. The results showed that OLA1 mRNA and protein were elevated in GC tissues. High expression of OLA1 was significantly associated with aggressive features, such as tumour size, lymph-node-metastasis and tumour-nodus-metastases stage ( p = 0.0146, p = 0.0037, p < 0.001, respectively). Moreover, high levels of OLA1 predicted worse overall survival. Multivariate Cox regression analysis indicated that high expression of OLA1 was an independent prognostic factor for poor overall survival ( p = 0.009). Additionally, OLA1 expression was positively correlated with Snail, and a combination of them revealed improved prognostic accuracy for GC patients. High expression of OLA1 predicts poor prognosis in GC patients and may be serviced as a novel target for GC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9667663","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Uterine sarcoma - time is worth its weight in gold. 子宫肉瘤——时间是无价的。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.127292

Dominik Pruski, Sonja Millert-Kalińska, Radosław Mądry, Klaudia Kotynia, Tadeusz Pawełek, Damian Rusek, Andrzej Zmaczyński, Robert Jach, Marcin Przybylski

引用次数: 0

CD98 expression can be a predictive factor of resistance to radiotherapy in head and neck squamous cell carcinoma. CD98的表达可能是头颈部鳞状细胞癌放疗耐药性的预测因素。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.128695

Hitomi Suzuki, Yohei Kawasaki, Shinsuke Suzuki, Takechiyo Yamada, Ayumi Ito, Maya Suzuki, Masahito Miura, Haruka Hatakeyama, Yasufumi Omori

{"title":"CD98 expression can be a predictive factor of resistance to radiotherapy in head and neck squamous cell carcinoma.","authors":"Hitomi Suzuki, Yohei Kawasaki, Shinsuke Suzuki, Takechiyo Yamada, Ayumi Ito, Maya Suzuki, Masahito Miura, Haruka Hatakeyama, Yasufumi Omori","doi":"10.5114/pjp.2023.128695","DOIUrl":"https://doi.org/10.5114/pjp.2023.128695","url":null,"abstract":"CD98 is a marker of cancer stem cells, and it regulates radiosensitivity in head and neck squamous cell carcinoma (HNSCC). The current study aimed to investigate whether CD98 can be used as a prognostic factor and marker of radioresistance. CD98 immunostaining was performed using biopsy specimens collected from patients diagnosed with HNSCC. The average period of postoperative monitoring was 31.6 months. The treatment options were radiation therapy with either cisplatin or cetuximab, and surgery. The participants were divided into groups of low and high fluorescence intensity. CD98 was an independent prognostic factor of radioresistance. In total, 103 patients were treated with chemoradiotherapy or bioradiotherapy. The overall survival rates of patients receiving chemoradiotherapy or bioradiotherapy were 69.2% in the low group and 36.2% in the high group. The progression-free survival rates were 60.0% and 24.6%, respectively. CD98 expression was considered an independent prognostic factor of overall survival and progression-free survival. In total, 99 patients underwent surgical treatment. The surgery group did not differ according to CD98 expression. Via CD98 immunostaining, sensitivity to radiotherapy can be determined in advance. In HNSCC, knowledge about sensitivity to radiotherapy can significantly improve prognosis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41157503","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Potential impact of HercepTest™ mAb PharmDx (Dako Omnis) (ge001) in breast cancer diagnosis. HercepTest的潜在影响™ mAbPharmDx（DakoOmnis）（ge001）在乳腺癌症诊断中的应用。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129352

Andrzej Marszałek, Agata Kubicka, Inga Jagiełło, Anna Malicka-Durczak

{"title":"Potential impact of HercepTest™ mAb PharmDx (Dako Omnis) (ge001) in breast cancer diagnosis.","authors":"Andrzej Marszałek, Agata Kubicka, Inga Jagiełło, Anna Malicka-Durczak","doi":"10.5114/pjp.2023.129352","DOIUrl":"https://doi.org/10.5114/pjp.2023.129352","url":null,"abstract":"The HER2 gene is a biomarker for breast cancer prognosis and treatment. Overexpression of HER2 protein determined by immunohistochemistry (IHC) or amplification of the HER2 gene determined by fluorescence in situ hybridization (FISH) is a condition for qualifying patients for anti-HER2 therapy. Due to the high toxicity of anti-HER2 treatment, proper patient selection is essential. In our study we compared 40 cases with IHC staining of HER2 antibody determined by Ventana PATHWAY anti-HER2/neu antibody (4B5) as HER2 2+ with the new antibody (HercepTest™ mAb PharmDx [Dako Omnis] [GE001]). Then using a double-blind study we compared the (IHC) evaluation with FISH results. In 65% of cases (26/40) the IHC 2+ score remained unchanged, in 32.5% of cases (13/40) expression of HER2 protein after IHC with new antibody was indicated as 3+ score, and in one case we observed a decrease of HER2 protein expression to 1+. In all cases but one, in which we found IHC HER 3+ with new antibody, there was FISH amplification. We have reason to believe that the new antibody will reduce the diagnostic time and avoid unnecessary costs. Due to the small study group, further investigation is needed.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41164504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Cutaneous metastasis of rectal adenocarcinoma – a case report and literature review 直肠腺癌皮肤转移1例报告并文献复习

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.128664

Vesa Alina, O. Maghiar, Laura Maghiar, Romina Cuc, O. Pop, A. Paşcalău, Monica Boros, A. Maghiar

引用次数: 0

Pleural mesothelioma metastasis of the scalp. 头皮的胸膜间皮瘤转移。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.134322

Tugcem Bicak, Selver Ozekinci, İbrahim İbiloğlu

引用次数: 0

Nuclear expression of Ku70/80 is associated with CHEK2 germline mutations in breast cancer. Ku70/80的核表达与癌症CHEK2种系突变相关。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129518

Rosik Jakub, Machaj Filip, Bodnar Daniel, Hybiak Jolanta, Huzarski Tomasz, Cybulski Cezary, Lubiński Jan, Domagała Wenancjusz, Paweł Domagała

{"title":"Nuclear expression of Ku70/80 is associated with CHEK2 germline mutations in breast cancer.","authors":"Rosik Jakub, Machaj Filip, Bodnar Daniel, Hybiak Jolanta, Huzarski Tomasz, Cybulski Cezary, Lubiński Jan, Domagała Wenancjusz, Paweł Domagała","doi":"10.5114/pjp.2023.129518","DOIUrl":"https://doi.org/10.5114/pjp.2023.129518","url":null,"abstract":"Ku70/80 protein inhibitors reduce the repair of DNA double-strand breaks via the Ku70/80 pathway, so they can be used to treat cancers with Ku70/80 overexpression. Since the association of Ku70/80 with germline CHEK2 mutations in breast cancer is unknown, in this study we evaluated the expression of Ku70/80 in breast cancers with germline CHEK2 mutations. Immunohistochemistry with a Ku70/80 antibody on tissue microarrays from 225 CHEK2-associated breast cancers was used and automatically assessed with computerized image analysis. We report that the vast majority of breast cancers expressed high level of nuclear Ku70/80 and a small percentage of tumors (3.5%) were negative for Ku70/80 expression. There was a significant difference between the nuclear Ku70/80 expression in CHEK2-associated vs. CHEK2-non-associated breast cancers in all tumors (p = 0.009), and in the estrogen receptor (ER) positive subgroup of breast cancers (p = 0.03). This study is the first reporting an association of Ku70/80 expression with CHEK2 germline mutations in breast cancer. The results suggest that evaluation of Ku70/80 expression in breast cancer may improve the selection of breast cancer patients for Ku70/80 inhibitor therapy, and point to CHEK2-associated breast cancer and a subset of ER-positive breast cancer as potential suitable targets for such therapy.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41150944","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0