Polish Journal of Pathology最新文献_第5页

High mobility group box 1 attenuates aortic stenosis by modulating macrophages to reduce valvular calcification. 高迁移率组1通过调节巨噬细胞减少瓣膜钙化来减轻主动脉狭窄。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153975

Dong Zhao, Yun Zhao, Li-Na Luan

{"title":"High mobility group box 1 attenuates aortic stenosis by modulating macrophages to reduce valvular calcification.","authors":"Dong Zhao, Yun Zhao, Li-Na Luan","doi":"10.5114/pjp.2025.153975","DOIUrl":"https://doi.org/10.5114/pjp.2025.153975","url":null,"abstract":"Our previous study demonstrated that HMGB1 may suppress M1 macrophage polarisation and mitigate the progression of calcific aortic valve disease (CAVD). However, the role of HMGB1 in regulating macrophage-mediated valvular calcifi-cation remains to be further explored. Serum samples from healthy individuals and CAVD patients with varying severity were collected and analysed by ELISA. Immunofluorescence staining of human heart tissue arrays assessed macrophage infiltration in calcified valves. A macro-phage-aortic valve interstitial cell (haVIC) co-culture system was used to examine the effects of reHMGB1-treated macrophages. RUNX2 and osteopontin mRNA expression were measured by RT-qPCR, and alkaline phosphatase (ALP) staining was performed to evaluate calcification. HMGB1 levels were significantly reduced in severe CAVD patients than controls. Immunofluorescence staining revealed increased CD68 expression in calcified valve samples, indicating macrophage infiltration. In the macrophage-haVIC co-culture system, macrophages pretreated with reHMGB1 led to reduced RUNX2 mRNA expression and lower ALP activity in haVICs, suggesting a potential inhibitory effect of HMGB1 on valvular calcification. HMGB1 may have the potential to suppress inflammation and mitigate aortic valve calcification, making it a promising therapeutic target for preventing the progression of aortic stenosis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"141-150"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114754","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

FOXC1 expression profile in invasive breast carcinomas and its relationship with prognostic parameters. 浸润性乳腺癌中FOXC1表达谱及其与预后参数的关系

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153969

Özge Bozkurt Öztürk, Remzi Arslan

{"title":"FOXC1 expression profile in invasive breast carcinomas and its relationship with prognostic parameters.","authors":"Özge Bozkurt Öztürk, Remzi Arslan","doi":"10.5114/pjp.2025.153969","DOIUrl":"https://doi.org/10.5114/pjp.2025.153969","url":null,"abstract":"This study aimed to investigate the expression profile of FOXC1 in molecular sub-types of invasive breast cancer. Additionally, it sought to explore the association between FOXC1 expression and clinicopathological prognostic parameters to eval-uate its potential diagnostic and therapeutic implications. A total of 122 invasive breast carcinoma cases from excision specimens were an-alysed. Immunohistochemical staining for FOXC1 was performed, with nuclear expression > 1% considered positive. The correlation between FOXC1 expression, molecular subtypes, and prognostic parameters was examined. A significant negative correlation was found between FOXC1 expression and ER, PR, and HER2 in 122 cases, while FOXC1 expression was notably higher in the triple-negative breast cancer (TNBC) subtype. Additionally, FOXC1 expression showed a significant positive correlation with nuclear grade, histological grade, Ki67 index, and prognostic stage. FOXC1 expression was found to be higher in TNBCs compared to other molecular subtypes, and FOXC1 expression was negatively correlated with hormone recep-tors and HER2. On the other hand, FOXC1 was significantly associated with high proliferation index, high-grade tumour, and prognostic stage. These findings sug-gest that high FOXC1 expression may indicate aggressive behaviour and may be a predictive marker for poor prognosis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"79-86"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114757","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Histopathological image analysis and enhanced diagnostic accuracy explainability for oral cancer detection. 组织病理学图像分析和提高口腔癌检测的诊断准确性。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153973

V P Gladis Pushparathi, Sylaja Vallee NarayanS R, Pratheeba R S, V Naveen

{"title":"Histopathological image analysis and enhanced diagnostic accuracy explainability for oral cancer detection.","authors":"V P Gladis Pushparathi, Sylaja Vallee NarayanS R, Pratheeba R S, V Naveen","doi":"10.5114/pjp.2025.153973","DOIUrl":"10.5114/pjp.2025.153973","url":null,"abstract":"Deep learning (DL) has transformed medical imaging, particularly in the realm of oral cancer (OC) diagnosis using histopathological images. Timely detection of OC is es-sential for enhancing precision medicine and saving lives. However, incorrect diagnosis may impede effective treatment. In this study, we have proposed a DL model for OC classification, enhanced diagnosis decision-making and interpretability. We achieve this by starting with colour normalisation of histopathology images using the Vaha-dane 3-stain parameter normalisation and watershed segmentation method, followed by tiling and augmentation. Key features are selected using the weighted Fisher score (WFS) to address class imbalance. The U-Net classifier has been improved by using feature-based inputs instead of full images, reducing computational complexity and training time. The integration of Vahadane normalisation for consistent preprocessing across samples, WFS, and explainable artificial intelligence (XAI) addresses critical challenges in histopathological image analysis. The proposed model surpasses exist-ing approaches with a classification accuracy of 99.54%, and it outperforms Dense- Net201 and VGG10 in precision and reliability. The efficiency in handling imbal-anced datasets and explainability features make it suitable for early precise OC detec-tion, which can reduce diagnostic errors and enhance treatment outcomes.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"120-130"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114764","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

An unexpected leprosy diagnosis in Nordic patient with skin lesions. 一个意外的麻风病诊断北欧患者的皮肤病变。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158044

Arnhild Binde, Johannes Hendricus van der Stoep, Camilla Sivesind Mehlum, Monika Aneta Dzieniecka

引用次数: 0

TROP-2 expression in triple-negative and human epidermal growth factor receptor 2 enriched breast cancers and its relationship with clinicopathologic parameters. TROP-2在三阴性和人表皮生长因子受体2富集乳腺癌中的表达及其与临床病理参数的关系

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156555

Hale Kivrak, Duygu Simsek, Egemen Akincioglu, Ilke Seçinti, Gül Keskin, Ismail Erturk, Murat Demiriz

{"title":"TROP-2 expression in triple-negative and human epidermal growth factor receptor 2 enriched breast cancers and its relationship with clinicopathologic parameters.","authors":"Hale Kivrak, Duygu Simsek, Egemen Akincioglu, Ilke Seçinti, Gül Keskin, Ismail Erturk, Murat Demiriz","doi":"10.5114/pjp.2025.156555","DOIUrl":"10.5114/pjp.2025.156555","url":null,"abstract":"TROP-2 is a transmembrane calcium signaling molecule that is detected at a high rate (63%) in breast cancers. There are conflicting data on its relationship with subtypes, clinical, and pathological data in breast cancer. TROP-2 expression levels were evaluated by immunohistochemistry, and the H-score method was used to analyze the data. This evaluation was conducted on a total of 79 patients diagnosed with triple-negative breast cancer (TNBC) and human epidermal growth factor receptor 2 (HER2) positive (HER2-E) breast cancer. The study also investigated the relationship between TROP-2 expression levels and the clinical-pathological features observed in patients diagnosed with TNBC and HER2-E. A total of 62 TNBC (78.5%) and 17 HER2-E (21.5%) cases were analyzed in the study. The presence of high TROP-2 (H-score > 100) was detected in 87% of the TNBC group and 94% of the HER2-E group. Among the pathological parameters, only low H-score values were found to have a statistically significant correlation with mucinous morphology; however, no significant correlation was found with other pathological parameters, including CerbB2 expression status. No significant relationship was found between H-score and clinical parameters. Furthermore, TROP-2 expression in HER2-E cancers is notably elevated. Further studies with larger series are required to clarify expression rates in mucinous tumors.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 3","pages":"248-256"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145998947","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Does SOX-2 expression have a prognostic value in triple-negative breast cancer? SOX-2表达在三阴性乳腺癌中有预后价值吗？

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149426

Ismail Guzelis, Betul Bolat Kucukzeybek, Merve Gursoy, Yeliz Yilmaz, Yuksel Kucukzeybek

{"title":"Does SOX-2 expression have a prognostic value in triple-negative breast cancer?","authors":"Ismail Guzelis, Betul Bolat Kucukzeybek, Merve Gursoy, Yeliz Yilmaz, Yuksel Kucukzeybek","doi":"10.5114/pjp.2025.149426","DOIUrl":"https://doi.org/10.5114/pjp.2025.149426","url":null,"abstract":"Triple-negative breast cancer (TNBC) is the most aggressive subtype of breast cancer. At the cell of origin level, cancer stem cells (CSC) are the tumour initiators in breast cancer. SRY-box transcription factor 2 (SOX-2) is a CSC marker that plays a role in tumourigenesis. The objective of this study was to evaluate the association of SOX-2 expression with histopathological parameters and clinical outcomes in TNBC patients. The study included 95 TNBC cases. An in vitro diagnostic SOX-2 antibody was applied to the tumoural slides in a validated automated stainer. The expression of SOX-2 was defined as a SOX-2 H-score ≥ 1. The expression of SOX-2 was observed in 29 cases (30.5%). At a median follow-up of 76 months, SOX-2 expression was not associated with overall or disease-free survival. R-based statistical analysis determined a SOX-2 H-score cut-off of 2. Although the overall and disease-free survival rates of cases with an H-score ≥ 3 were lower than the others, the differences were not statistically significant. The percentage of SOX-2 staining is typically low, as only 1% of tumour cells exhibit CSC characteristics. In conclusion, the prognostic significance of SOX-2 could become clear in a larger group of TNBC patients using standardized methodologies.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"1-9"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776747","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

In memoriam: Paweł Piotr Liberski.

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158045

Beata Sikorska, Radzisław Kordek

引用次数: 0

Better pathology education during undergraduate education: Clinical dentists' expectations. 在本科教育中加强病理学教育：临床牙医的期望。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153976

Songül Sahin

{"title":"Better pathology education during undergraduate education: Clinical dentists' expectations.","authors":"Songül Sahin","doi":"10.5114/pjp.2025.153976","DOIUrl":"https://doi.org/10.5114/pjp.2025.153976","url":null,"abstract":"The education curriculum of the faculty of dentistry includes both general pathol-ogy and oral pathology courses. In our planned study, we aimed to determine the needs of dentists who graduated from the faculty of dentistry and received special-isation training for pathology information in their active professional lives and the importance of pathology education according to their fields of specialisation. Survey questions: Applications were made to 115 dentists who graduated from the faculty of dentistry, received specialist training, and worked in the clinic. While the benefit of pathology training in the dentist's professional life was oral and maxillofacial surgery, it was followed by periodontology, radiology, and oral diagnosis specialties. They also stated that they sent samples and evaluated reports in the fields of oral and maxillofacial surgery, periodontology, radiology, and oral diagnosis. In our study, the areas where specialist physicians benefit from pathology training in the clinic are those where the subjects overlap more with the sections in the training curriculum. It is necessary to provide adequate training in basic medical sciences to train dentists as physicians who do not perceive the patient only in terms of mouth and teeth, but can evaluate the patient as a whole and provide the right guidance at the right time. Integration should be ensured between basic sciences, dentistry, and clinical sciences. For pathology education to be permanent and useful, a curriculum should be designed with innovative teaching methods that are department-specific and practice-based, appropriate to the needs of the clinic. Dentistry graduates can improve patient outcomes and enhance their personal de-velopment by deepening their understanding of pathology education.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"151-157"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114744","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Impact of age and histological type on programmed death-ligand 1 expression in non-small cell lung cancer - a single-center analysis of 1,606 cases. 年龄和组织学类型对非小细胞肺癌中程序性死亡配体1表达的影响——一项1606例病例的单中心分析

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.157974

Anna Szumera-Ciećkiewicz, Piotr Wiśniewski, Agnieszka Tomczyk-Szatkowska, Magdalena Rosińska, Dorota Pierzchała, Joanna Owczarek, Kinga Winiarczyk, Patrycja Wołoszyn, Maciej Krzakowski, Magdalena Knetki-Wróblewska

{"title":"Impact of age and histological type on programmed death-ligand 1 expression in non-small cell lung cancer - a single-center analysis of 1,606 cases.","authors":"Anna Szumera-Ciećkiewicz, Piotr Wiśniewski, Agnieszka Tomczyk-Szatkowska, Magdalena Rosińska, Dorota Pierzchała, Joanna Owczarek, Kinga Winiarczyk, Patrycja Wołoszyn, Maciej Krzakowski, Magdalena Knetki-Wróblewska","doi":"10.5114/pjp.2025.157974","DOIUrl":"10.5114/pjp.2025.157974","url":null,"abstract":"Programmed death-ligand 1 (PD-L1) guides immune checkpoint inhibitor use in non-small cell lung cancer (NSCLC), yet its variation by age and histology remains uncertain. We retrospectively evaluated 1,606 consecutive NSCLC cases (2017-2022) with PD-L1 immunohistochemistry (IHC) on formalin-fixed, paraffin- embedded samples. Patients were grouped by age (< 60, 60-79, ≥ 80 years) and tumor proportion score (TPS), categorized as < 1%, 1-49%, or ≥ 50%. Associations were tested using the ² test, and independent predictors were identified using multinomial logistic regression. High PD-L1 expression (TPS ≥ 50%) occurred in 33.6% of patients, intermediate (1-49%) in 23.6%, and negative (< 1%) in 42.7%. Programmed death-ligand 1 expression ≥ 1% was most frequent in squamous cell carcinoma (63.0%), followed by adenocarcinoma (55.0%), and was least common in large cell carcinoma (36.0%; p = 0.002). Overall proportions of PD-L1 ≥ 1% did not differ significantly by age. However, patients aged ≥ 80 had nearly twice the likelihood of high expression compared to those < 60 (relative risk ratio, 1.92; 95% CI: 1.11-3.34; p = 0.02), independent of the histotype. Programmed death-ligand 1 expression in NSCLC shows distinct histotype-related patterns and a modest, age-related trend toward higher values in the oldest group. These data support routine PD-L1 assessment and suggest that advanced age alone should not preclude consideration of immunotherapy. Findings may inform trial design and real-world treatment decision-making.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 3","pages":"177-186"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145999514","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Molecular profiling of sporadic medullary thyroid carcinomas - a next-generation sequencing-based study. 散发性甲状腺髓样癌的分子谱分析——新一代基于测序的研究。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.156557

Serdar Altinay, Altan Kara, Mustafa Gülbağcı, Ahmet Cem Dural, Şaban Tekin, Tuğba Köprülü, Nisa Erdoğan, Meral Mert, Ülkan Çelik, Mustafa Turan

{"title":"Molecular profiling of sporadic medullary thyroid carcinomas - a next-generation sequencing-based study.","authors":"Serdar Altinay, Altan Kara, Mustafa Gülbağcı, Ahmet Cem Dural, Şaban Tekin, Tuğba Köprülü, Nisa Erdoğan, Meral Mert, Ülkan Çelik, Mustafa Turan","doi":"10.5114/pjp.2025.156557","DOIUrl":"10.5114/pjp.2025.156557","url":null,"abstract":"Medullary thyroid carcinomas (MTCs) are 75% sporadic and 25% hereditary. This study aimed to determine the histopathological parameters and molecular changes of sporadic MTCs in a university hospital by targeted next-generation sequencing (NGS) including 62 genes. All RET mutations were missense mutations. EIF1AX was suggested by artificial intelligence as a gene of interest for further analysis; subsequent testing revealed a pathogenic missense mutation in this gene in a patient with advanced-stage disease, who died at the 25th month of follow-up due to liver metastasis. We identified different gene mutations that could be associated with nodal metastasis in the presence or absence of RET mutation. We identified mutations that may be involved in tumour progression and have prognostic significance, such as HRAS, MAP3K1, and EIF1AX. We observed KDR mutation in this cohort. Although driver mutations in sporadic medullary thyroid carcinoma (sMTC) mostly come from targeted NGS data in tumours from patients with localised disease, NGS findings can also be used for therapeutic purposes in advanced-stage sMTC cases with progressive local-regional or distant metastatic disease. We believe that additional studies should be conducted with a larger number of patients so that the findings can be included in the treatment guidelines to be prepared.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 3","pages":"195-213"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145999515","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0