Polish Journal of Pathology最新文献_第6页

The prognostic role of epidermal growth factor receptor mutation in completely resected ampullary adenocarcinoma. 表皮生长因子受体突变在完全切除的膀胱腺癌中的预后作用

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.127044

Umut Varol, Ebru Cakır, Safiye Aktas, Zekiye Sultan Altun, Fatma Husniye Dilek, Osman Butun, Tarık Salman, Ayse Gulden Diniz Unlu, Ahmet Alacacioglu, Isil Somali

{"title":"The prognostic role of epidermal growth factor receptor mutation in completely resected ampullary adenocarcinoma.","authors":"Umut Varol, Ebru Cakır, Safiye Aktas, Zekiye Sultan Altun, Fatma Husniye Dilek, Osman Butun, Tarık Salman, Ayse Gulden Diniz Unlu, Ahmet Alacacioglu, Isil Somali","doi":"10.5114/pjp.2023.127044","DOIUrl":"10.5114/pjp.2023.127044","url":null,"abstract":"The aim of this study is to make a differential diagnosis and prognosis of the ampullary adenocarcinoma subtypes. We also investigated the role of prognostic markers PD-1 and PD-L1, and epidermal growth factor receptor (EGFR). Local or locally advanced stage ampullary adenocarcinoma patients who had undergone pancreaticoduodenectomy at the time of diagnosis were included. MUC1, MUC2, MUC5AC, CDX2, CK7, CK20, PD-1, and PDL-1 were analysed immunohistochemically, and EGFR was analysed by real-time polymerase chain reaction. According to histopathological and immunohistochemical evaluation, we found 27 patients as pancreatobiliary type and 56 patients as intestinal type adenocarcinoma. The median survival of patients with intestinal and pancreatobiliary type adenocarcinoma was 23 months and 76 months ( p = 0.201), respectively. When the survival of PD1-positive ( n = 23) and PD-L1-positive ( n = 18) patients were compared with the patients with negative staining ( n = 60, n = 65), no significant difference was found. Epidermal growth factor receptor mutation was detected in a total of 6 patients, and 5 of these 6 mutations were shown in intestinal type tumours and one in a pancreatobiliary type tumour. A significant difference was determined in terms of overall survival for the patients with EGFR mutations compared to those without ( p = 0.008). In conclusion, we could reveal the prognostic significance of EGFR mutation, which is also a target molecule.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 1","pages":"18-28"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9620504","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Evaluation of the cytogenetic profile in patients with acute leukaemia. 急性白血病患者细胞遗传学特征的评估。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129425

Nagaraj V Kulkarni, Vijith V Shetty, Kishan Prasad Hl, Meenakshi Arumugam, Rajesh Krishna, Reshma A Shetty, Akanksha A Kalal, D Prashanth Shetty

{"title":"Evaluation of the cytogenetic profile in patients with acute leukaemia.","authors":"Nagaraj V Kulkarni, Vijith V Shetty, Kishan Prasad Hl, Meenakshi Arumugam, Rajesh Krishna, Reshma A Shetty, Akanksha A Kalal, D Prashanth Shetty","doi":"10.5114/pjp.2023.129425","DOIUrl":"https://doi.org/10.5114/pjp.2023.129425","url":null,"abstract":"Acute leukaemia (AL) is a heterogeneous neoplastic disease that occurs by the growth of abnormal lymphoid and myeloid cells in the bone marrow and blood leading to acute myeloid leukaemia (AML) and acute lymphocytic leukaemia (ALL). Conventional cytogenetics is a characteristic technique to hunch chromosomal abnormalities, it helps in the diagnosis and therapeutic approach of the disease by the molecular cytogenetics technique of fluorescence in situ hybridization (FISH). Chromosomal abnormalities in AL are performed by karyotyping to confirm specific chromosomal abnormalities using FISH. The descriptive study included 42 clinically diagnosed AL patients. Karyotyping analysis was performed using the standard Giemsa banding procedure. To confirm specific chromosomal abnormalities and all culture failure (CF) cases, FISH was done. Among 42 cases, 29 (69.4%) males and 13 (30.9%) females, AML comprised 22 (52.38%) cases, ALL 14 (33.33%) cases, and AL 6 (14.2%) cases. Normal karyotype was found in 18 (42.85%), abnormal karyotype in 16 (39.09%), and 8 (19.09%) were CF. Specific abnormalities of t(15;17), hyperdiploidy; t(3;3) with monosomy 7 in; del(9q22); del(2p); del(17p); del(Xq); 1~2 dmin; der(3); +11, +13 and composite karyotype. Hypodiploidy was strongly associated with AL, which signifies the loss of chromosomes causing potential risk. Composite karyotype, rare t(3;3) double minutes, +11,+13, del(9q), and del(Xq) were the novel findings reported in the South Canara region of Karnataka. Despite other molecular techniques, conventional cytogenetics remains the baseline in the diagnosis of malignancies.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 2","pages":"89-97"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41179024","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Lowered expression level of INSC predicts poor prognosis in patients with colon cancer. INSC表达水平降低可预测癌症患者预后不良。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129301

Yan Li, Shugao Tian, Jing Ran, Xiaofan Han

{"title":"Lowered expression level of INSC predicts poor prognosis in patients with colon cancer.","authors":"Yan Li, Shugao Tian, Jing Ran, Xiaofan Han","doi":"10.5114/pjp.2023.129301","DOIUrl":"https://doi.org/10.5114/pjp.2023.129301","url":null,"abstract":"The aim of this study was to investigate the prognostic value of inscuteable spindle orientation adaptor protein (INSC) in colon cancer (CC). Firstly, transcriptional change of INSC was analysed using the data from public databases. Next, INSC protein expression was assessed by immunohistochemistry. Its correlation with clinicopathological features and the prognostic values of patients were also investigated. Then, an INSC-based nomogram was built to predict CC prognosis. Compared to normal tissues, INSC was significantly downregulated at the transcriptional level in CC tissues. A low INSC mRNA level not only positively correlated with TNM stage (tumour-nodus-metastases), advanced T stage, and N stage, but also with the shorter 5- and 8-year overall survival (OS) and disease-specific survival. Concerning protein level, INSC downregulation was confirmed in CC samples. In terms of the correlation with N stage and 5- and 8-year OS, it was also consistent with mRNA levels. Cox regression analysis indicated that INSC protein expression was an independent prognostic factor for OS. The nomogram showed better prognostic accuracy and clinical net benefit for 5-year OS than TNM staging. Altogether, downregulation of INSC is related to inferior clinicopathological features and patient outcomes, and it may be a novel independent prognostic biomarker in CC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 2","pages":"109-121"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41149043","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Single-minded homolog 2 as a potential prognostic signature and assessment of its correlation with immune cell infiltration in pancreatic cancer. 作为潜在预后特征的单基因同源物 2 及其与胰腺癌免疫细胞浸润的相关性评估。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.134317

Qian Hu, Na Wen, Fang Li, Dujiang Yang, Chao Yue, Zuowei Wu, Mao Li, Huimin Lu

{"title":"Single-minded homolog 2 as a potential prognostic signature and assessment of its correlation with immune cell infiltration in pancreatic cancer.","authors":"Qian Hu, Na Wen, Fang Li, Dujiang Yang, Chao Yue, Zuowei Wu, Mao Li, Huimin Lu","doi":"10.5114/pjp.2023.134317","DOIUrl":"10.5114/pjp.2023.134317","url":null,"abstract":"Single-minded homolog 2 (SIM2) has been identified as a potential contributor to the development of solid tumors. Despite this, there is a lack of comprehensive research regarding its biological role and underlying mechanism within pancreatic cancer (PC), as well as its prognostic impact. This study systematically evaluated the expression level and clinical significance of SIM2 in patients with PC using various databases, including The Cancer Genome Atlas, KM Plotter, and gene expression profiling interactive analysis. To investigate the relationship between SIM2 expression and immune cell infiltration, we conducted ESTIMATE and single-sample gene set enrichment analysis (ssGSEA) analyses. Single-minded homolog 2 was up-regulated in patients with PC. Pancreatic cancer patients with higher SIM2 expression had poorer overall survival rates. Gene set enrichment analysis results suggested that SIM2 may have a significant impact on the progression of PC and the regulation of immune responses. According to the ssGSEA algorithm, SIM2 has a negative correlation with the levels of infiltrating TFH, mast cells, and pDC. Our study demonstrated that SIM2 serves as a biomarker, and is associated with both prognosis and immune infiltration in PC. This provides a solid foundation for future investigations into the precise role of SIM2 in the carcinogenesis and progression of PC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 4","pages":"232-247"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140111966","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129014

Pınar Savaş, Gürdeniz Serin, Pınar Gürsoy, Osman Zekioğlu, Necmettin Özdemir

{"title":"Prognosis-related novel immunostaining pattern for programmed cell death ligand 1 and prognostic value of tumour-infiltrating lymphocytes in triple-negative breast cancer.","authors":"Pınar Savaş, Gürdeniz Serin, Pınar Gürsoy, Osman Zekioğlu, Necmettin Özdemir","doi":"10.5114/pjp.2023.129014","DOIUrl":"https://doi.org/10.5114/pjp.2023.129014","url":null,"abstract":"This study aims to determine the prognostic significance of programmed cell death ligand 1 (PD-L1) expression and tumour-infiltrating lymphocytes (TILs) in triple- negative breast cancer (TNBC). PD-L1 expression and TIL percentage were determined in TNBCs that did not receive neoadjuvant therapy. The relationship between PD-L1 expression and the percentage of TILs with survival was investigated. The presence of intratumoural PD-L1-positive tumour-infiltrating immune cells (TIICs) in tumours with ≥ 1% PD-L1 expression was identified as a new PD-L1 evaluation parameter. The presence of intratumoural PD-L1-positive TIICs as a new parameter in PD-L1-positive cases increased overall survival. The percentage of TILs increased in both overall and distant metastasis-free survival (p = 0.040 and p = 0.006, respectively). As a result, it was found that the risk of death was increased 5.18-fold (p = 0.013) in patients without intratumoural PD-L1-positive TIICs. This risk of death was calculated to be 5.40-fold higher in patients with TIL percentage ≤ 10% than in those with > 40% (p = 0.024), and the risk of distant metastasis was calculated to be 11.95 times higher. In our study, we discovered that the percentage of TILs made a statistically significant difference in TNBC survival. The presence of intratumoural PD-L1-positive TIICs in PD-L1-positive cases significantly increased survival.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 2","pages":"65-74"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"41155198","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Prognostic significance of PD-L1 expression in pancreatic cancer: evidence from an updated meta-analysis. 胰腺癌中PD-L1表达的预后意义:来自最新荟萃分析的证据

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132220

Chenchen Liu, Lijuan Fan, Qian Wu, Yingjie Shi, Xuan Sun

{"title":"Prognostic significance of PD-L1 expression in pancreatic cancer: evidence from an updated meta-analysis.","authors":"Chenchen Liu, Lijuan Fan, Qian Wu, Yingjie Shi, Xuan Sun","doi":"10.5114/pjp.2023.132220","DOIUrl":"10.5114/pjp.2023.132220","url":null,"abstract":"Recent studies revealed that programmed cell death ligand 1 (PD-L1) expression was associated with unfavorable prognosis in various solid tumors, but its clinical relevance for pancreatic cancer has not yet been well established. This meta-analysis summarizes the potential prognostic value of PD-L1 in pancreatic cancer. A quantitative meta-analysis was performed by a systematic search of databases including PubMed, EMBASE, Web of Science, Cochrane library, Scopus and Ovid for eligible studies on the prognostic significance of PD-L1 in pancreatic cancer patients. Pooled hazard ratios (HRs) and their 95% confidence intervals (CIs) were calculated to evaluate the strength of the link between PD-L1 expression and clinical prognosis of patients. Seventeen eligible studies with 2669 patients were included in our study. A significant association was observed between PD-L1 abundance and poor overall survival (OS) of patients with pancreatic cancers, with a pooled hazard ratio (HR) of 1.902, 95% CI: 1.657-2.184. Sensitivity analysis confirmed the reliability of our results. Subgroup analysis shows that differences in regions and detection methods of PD-L1 did not change the overall predictive value of PD-L1 for poor prognosis in pancreatic cancer patients. This meta-analysis indicated that the expression of PD-L1 is associated with a worse OS in pancreatic cancer patients. Additionally, PD-L1 may act as a potential parameter for predicting poor prognosis and thus providing a promising target for anticancer therapy in pancreatic cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 3","pages":"151-160"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. 香菇多糖对人星形细胞瘤U251细胞增殖和凋亡的影响。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2022.119264

Min Yuan, Chengyu Li, Xiang Xiao, Dengfeng Wan, Bin Xi, Xingxing Jiang, Jianzhong Zhang

{"title":"Effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells.","authors":"Min Yuan, Chengyu Li, Xiang Xiao, Dengfeng Wan, Bin Xi, Xingxing Jiang, Jianzhong Zhang","doi":"10.5114/pjp.2022.119264","DOIUrl":"10.5114/pjp.2022.119264","url":null,"abstract":"Aim of the study: Aim of the study is To investigate the effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. Lentinan was dissolved in DMEM complete medium to form different concentrations (0, 25, 50, 100, 200, 400, 500, 600 µg/ml). CCK8 was used to detect the effect of lentinan with different concentrations on proliferation of human astrocytoma U251 cells, and the expression of Ki-67 was detected by immunofluorescence. In addition, the effect of different concentrations of lentinan on apoptosis of human astrocytoma U251 cells was detected by flow cytometry. Compared with the blank control group, 50 and 100 µg/ml lentinan significantly promoted proliferation of human astrocytoma U251 cells. When the concentration is more than 100 µg/ml, the cell activity gradually decreases, and the cell activity is the lowest when the concentration is 600 µg/ml. In addition, the low concentration lentinan (25, 50, and 100 µg/ml) had no significant effect on apoptosis of human astrocytoma U251 cells. However, lentinan above 200 µg/ml significantly promoted apoptosis of human astrocytoma U251 cells and had a concentration gradient effect, and the highest apoptosis rate was at 600 µg/ml.Conclusions: Lentinan can effectively inhibit proliferation and promote apoptosis of human astrocytoma U251 cells.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":" ","pages":"136-140"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10629671","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Incidental lymphangioleiomyomatosis in pelvic lymph nodes associated with Malignant neoplasm of the ovary - two case reports. 盆腔淋巴结伴卵巢恶性肿瘤伴淋巴管平滑肌瘤病2例报告。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.129181

Rupei Ye, Yehui Liao, Li Luo, Xiuli Xiao

引用次数: 0

Potential significance of podoplanin immunohistochemical expression in papillary thyroid carcinoma. podoplanin免疫组化表达在甲状腺乳头状癌中的潜在意义。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132222

Rana M Khalil, Nafissa M El Badawy, Wesam M Osman, Lobna S Shash, Fatma S Hafez

{"title":"Potential significance of podoplanin immunohistochemical expression in papillary thyroid carcinoma.","authors":"Rana M Khalil, Nafissa M El Badawy, Wesam M Osman, Lobna S Shash, Fatma S Hafez","doi":"10.5114/pjp.2023.132222","DOIUrl":"10.5114/pjp.2023.132222","url":null,"abstract":"Podoplanin (PDPN) is a lymphatic endothelial marker expressed by a range of human malignancies in which it has been shown to contribute to tumor progression and metastasis. However, there is a lack of the studies, examining the function of PDPN in thyroid cancer. The current study was performed to explore the possible diagnostic value of PDPN expression in papillary thyroid cancer (PTC) and to evaluate the marker's potential for prediction of regional lymph node metastasis. Lymphatic vascular density (LVD) and the stromal/cancer-associated fibroblasts (CAFs), labeled by PDPN, were examined in PTC compared to the other thyroid lesions. The current study included 50 cases of PTC and 50 cases of non-PTC thyroid lesions. Immunohistochemical staining was performed using monoclonal PDPN antibodies. Podoplanin expression was scored as positive and negative. Podoplanin expression was found in 36% of PTC cases, but it was not found in benign, low risk (borderline), or malignant lesions other than PTC. Furthermore, lymph node metastasis was significantly correlated with PDPN expression, LVD and CAFs (p-values < 0.00001, < 0.001 and 0.0002 respectively). These findings support the diagnostic utility of PDPN expression in PTC and its predictive value for LN metastasis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"74 3","pages":"171-181"},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"89720233","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Primary epithelioid angiosarcoma of endometrium. 原发性子宫内膜上皮样血管肉瘤。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132302

Selim Sevim, Ekin Gedik, Hilal Ozakinci, Gulsah Kaygusuz, Duygu Enneli

引用次数: 0