Polish Journal of Pathology最新文献_第7页

Immunohistochemical expression and association of hypoxia-inducible factor 1a and carbonic anhydrase IX in colorectal cancer. 低氧诱导因子1a和碳酸酐酶IX在结直肠癌中的免疫组织化学表达及关联。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149408

Veronika Cígerová, Marian Adamkov, Marián Grendár, Veronika Mešťanová

{"title":"Immunohistochemical expression and association of hypoxia-inducible factor 1a and carbonic anhydrase IX in colorectal cancer.","authors":"Veronika Cígerová, Marian Adamkov, Marián Grendár, Veronika Mešťanová","doi":"10.5114/pjp.2025.149408","DOIUrl":"https://doi.org/10.5114/pjp.2025.149408","url":null,"abstract":"The presented work focuses on hypoxia-inducible factor 1a (HIF-1a) and carbonic anhydrase IX (CA IX) in colorectal cancer (CRC). The HIF-1a protein shows increased expression due to hypoxia, resulting in up-regulation of CA IX, which is involved in the survival of hypoxic cancer cells in the tumour microenvironment, with overexpression in various types of carcinomas. HIF-1a and CA IX immunohistochemical analysis was performed on 111 CRC samples. The primary goal was to determine the correlation of expression of proteins with clinical-morphological parameters and mutual correlation of the proteins in question. The HIF-1a expression was detected in 72.1% of CRC samples with exclusive nuclear localisation. The immunoreaction intensity was predominantly strong. Carbonic anhydrase IX protein was expressed in 75.7% of cases. The membrane positivity and strong immunoreaction intensity were mainly noticed. No statistically significant correlation between the expression of studied proteins and clinical-morphological parameters was confirmed. However, the results proved a statistically significant correlation in mutual co-localisation of given proteins. Despite contradictory scientific data, our findings suggest a mutual correlation between HIF-1a and CA IX in CRC. The presented hypothesis that their overexpression may represent a potential new therapeutic target in colorectal carcinogenesis might unveil novel strategies in disease development.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"16-24"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776749","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Coexistence of head and face Merkel cell and squamous cell carcinomas in the elderly population - a case report and literature review. 老年人群头面部默克尔细胞和鳞状细胞癌共存1例报告并文献复习

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149383

Shiwei Zhang, Hongliang Ji, Pan Qin

引用次数: 0

Identification of metabolism regulators as diagnostic markers for ulcerative colitis and their correlation with immune infiltration. 代谢调节因子作为溃疡性结肠炎诊断标志物的鉴定及其与免疫浸润的相关性。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.153972

Qilong Duan, Peng Liu, Hualei Chen, Yuanyuan Ding, Xiaoming Xu

{"title":"Identification of metabolism regulators as diagnostic markers for ulcerative colitis and their correlation with immune infiltration.","authors":"Qilong Duan, Peng Liu, Hualei Chen, Yuanyuan Ding, Xiaoming Xu","doi":"10.5114/pjp.2025.153972","DOIUrl":"https://doi.org/10.5114/pjp.2025.153972","url":null,"abstract":"This study determined novel metabolism-related diagnostic biomarkers for ulcer-ative colitis (UC) and assessed their correlation with immune cell infiltration levels. Transcriptome data of UC was downloaded from the Gene Expression Omnibus (GEO) database, metabolism-related genes were summarised from the Gene Set Enrichment Analysis (GSEA) database. A total of 537 metabolism-related differen-tially expressed genes (DEGs) in UC were applied to functional enrichment analy-sis. We processed least absolute shrinkage and selection operator (LASSO) regres-sion analysis and support vector machine-recursive feature elimination (SVM-RFE). We obtained 6 potential metabolism-related diagnostic biomarkers (CHST13, ETNK1, LPCAT1, PDE6A, PLA2G2A, and UGT2A3). Expression patterns and diagnostic ROC curves were depicted in both the training and testing co-horts to verify their diagnostic value. Immune infiltration analysis indicated that UC samples have more abundant infiltration levels of immune cells. Fur-thermore, the upregulated diagnostic biomarkers significantly positively cor-related with B cell memory, T cell CD4 memory activated, dendritic cells ac-tivated, etc., while the downregulated ones mainly significantly positively correlated with mast cells resting, NK cells activated, and macrophages M2. Our study primarily identified 6 metabolism regulators (CHST13, ETNK1, LP-CAT1, PDE6A, PLA2G2A, and UGT2A3) as potential diagnostic biomarkers for UC and determined their correlation with immune infiltration.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 2","pages":"110-119"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145114752","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Malignant transformation of the epithelial component of a Warthin tumour into adenocarcinoma. 沃辛肿瘤上皮成分向腺癌的恶性转化。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.149536

WenBin Gou, BeiWen Song, KeJia Tong, Yong Qiang Yang

{"title":"Malignant transformation of the epithelial component of a Warthin tumour into adenocarcinoma.","authors":"WenBin Gou, BeiWen Song, KeJia Tong, Yong Qiang Yang","doi":"10.5114/pjp.2025.149536","DOIUrl":"10.5114/pjp.2025.149536","url":null,"abstract":"To study the clinicopathological features of malignant transformation of the epithelial component of a Warthin tumour into adenocarcinoma. The histological and immunophenotypic features of the malignant transformation of the epithelial component of a Warthin tumour into adenocarcinoma were studied, and the literature was reviewed. The patient had a history of surgery for a Warthin tumour 3 years previously, and the typical histological manifestations of a Warthin tumour were observed under the microscope following biopsy. Scattered tumour tissue infiltration was observed in the lymphatic interstitium, and a transitional area of benign and malignant epithelial cells was observed locally. The tumour cells were solid, nested, and glandular and grew infiltratively in the salivary gland tissue. There was diffuse positive staining for CK7 and CK19 in the cytoplasm of tumour cells. CK5/6 was positively expressed in the cytoplasm of basal cells in the tumour tissue, and P63 was positively expressed in the basal cell nuclei. Ki-67 positivity reached approximately 10% in tumour tissues. The MAML2 fracture probe result was negative. The final diagnosis was malignant transformation of a Warthin tumour, with malignant transformation of epithelial components into adenocarcinoma. Malignant transformation of a Warthin tumour is rare, and the intraoperative histopathological diagnosis based on frozen sectioning should be made with caution. The key to diagnosis is to determine whether there is migration between benign and malignant epithelium.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"64-69"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776751","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Crosstalk between OPG/RANKL/RANK in bone marrow mesenchymal stem cells and Wnt/b-catenin pathway in prostate cancer cells regulates bone metastasis of prostate cancer. 骨髓间充质干细胞OPG/RANKL/RANK与前列腺癌细胞Wnt/b-catenin通路的串扰调控前列腺癌骨转移。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.150029

Shihua Ye, Qiongyun Lin, Meihua Deng, Shulong Huang, Changlin Mao, Jiabin Zhang, Wuming Zhan, Guangbing Chen

{"title":"Crosstalk between OPG/RANKL/RANK in bone marrow mesenchymal stem cells and Wnt/b-catenin pathway in prostate cancer cells regulates bone metastasis of prostate cancer.","authors":"Shihua Ye, Qiongyun Lin, Meihua Deng, Shulong Huang, Changlin Mao, Jiabin Zhang, Wuming Zhan, Guangbing Chen","doi":"10.5114/pjp.2025.150029","DOIUrl":"https://doi.org/10.5114/pjp.2025.150029","url":null,"abstract":"This study aimed to investigate whether the crosstalk between the osteoprotegerin (OPG)/receptor activator of nuclear factor-kB (RANK)/receptor activator of nuclear factor-kB ligand (RANKL) in bone marrow mesenchymal stem cells (BMSCs) and Wnt/b-catenin pathways in Pca cells regulates bone metastasis of PCa. Our study showed that there was increased OPG/RANKL/RANK and b-catenin expression in the tissue of PCa and its bone metastasis. This study further showed that RANKL knockdown in BMSCs or b-catenin knockdown in PC-3s blocked the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. Conversely, RANKL overexpression in BMSCs and b-catenin overexpression in PC-3s promoted the proliferation and migration of BMSCs and the proliferation, migration, and invasion of PC-3s in vitro. These data indicate that the RANKL pathway in BMSCs promoted the PC-3s invasion and the catenin pathway in PC-3s activated BMSCs with expression of cancer-associated fibroblast markers, which promoted the bone metastasis. This suggests that the interaction and crosstalk between BMSCs in bone microenvironment and PCa play a critical role in the exquisite tropism for Pca bone metastasis. Cancer therapies classically target tumour cells; however, based on this study, targeting BMSCs in bone microenvironment is a reasonable option for PCa therapy strategy.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 1","pages":"25-37"},"PeriodicalIF":0.6,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144776746","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Dedifferentiation in adenoid cystic carcinoma of the breast - an unexpected intraoperative finding and a diagnostic challenge. 乳腺腺样囊性癌的去分化-一个意外的术中发现和诊断挑战。

IF 0.6 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158973

Selcuk Cin, Ezgi Duman, Merve Cin

引用次数: 0

Vimentin 3 expression in odontogenic keratocysts - a molecular and immunohistochemical study. 牙源性角化囊肿中Vimentin - 3表达的分子和免疫组织化学研究。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.159041

Ipek Atak Secen, Heike Goebel, Emre Barış, Melanie Freifrau von Brandenstein, Sibel Elif Gültekin

{"title":"Vimentin 3 expression in odontogenic keratocysts - a molecular and immunohistochemical study.","authors":"Ipek Atak Secen, Heike Goebel, Emre Barış, Melanie Freifrau von Brandenstein, Sibel Elif Gültekin","doi":"10.5114/pjp.2025.159041","DOIUrl":"10.5114/pjp.2025.159041","url":null,"abstract":"Odontogenic keratocysts (OKC) are locally aggressive jaw lesions characterized by a high recurrence rate. Vimentin 3 (VIM3), a truncated splice variant of full-length vimentin (VIMFL), has recently emerged as a potential biomarker in odontogenic tumors. However, its role in OKC and association with recurrence remain unexplored. Formalin-fixed paraffin-embedded samples from OKC (n = 25) and dentigerous cysts (DC) (n = 25) were analyzed. Immunohistochemical staining for VIM3, VIMFL, p53, and Ki-67 was performed. In parallel, reverse transcription-quantitative polymerase chain reaction was used to assess mRNA expression levels of VIM3, VIMFL, and p53. While VIM3 expression did not significantly differ between OKC and DC, recurrent OKC exhibited significantly higher VIM3 labeling indices compared to non-recurrent cases (p = 0.040). This study demonstrates, for the first time, the expression of VIM3 in OKC. Although the findings are promising, further research with larger cohorts and functional validation is required to clarify the mechanistic role of VIM3 in odontogenic cyst biology.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"284-291"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576210","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Utility of BRAF immunohistochemistry for the detection of BRAF mutations in papillary thyroid carcinoma. Analysis of detailed histological subtypes. BRAF免疫组化检测甲状腺乳头状癌BRAF突变的应用详细的组织学亚型分析。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2025-01-01 DOI: 10.5114/pjp.2025.158888

Evren Uzun, Suna Erkilic

{"title":"Utility of BRAF immunohistochemistry for the detection of BRAF mutations in papillary thyroid carcinoma. Analysis of detailed histological subtypes.","authors":"Evren Uzun, Suna Erkilic","doi":"10.5114/pjp.2025.158888","DOIUrl":"10.5114/pjp.2025.158888","url":null,"abstract":"The BRAF mutation has been observed in 32-73% of papillary thyroid carcinomas. Despite the superiority of genetic tests for detecting BRAF mutations, they have inherent disadvantages. The objective of this study was to analyze the specificity and sensitivity of BRAF immunohistochemistry (IHC) that has been used in recent years and may serve as an alternative to genetic testing, in papillary thyroid carcinoma. A total of 103 cases of papillary thyroid carcinoma were analyzed for BRAF V600E mutation by real-time polymerase chain reaction (PCR) and IHC. The results demonstrated that the BRAF V600E mutation was identified in 55 (53.3%) of 103 cases by real-time PCR, while BRAF IHC positivity was observed in 65 (63.1%) of 103 cases. The study found that the specificity of BRAF IHC was 79.2%, while the sensitivity was 100%. The real-time PCR analysis identified 10 cases that were positive for BRAF IHC and exhibited wild-type BRAF. BRAF IHC can be utilized as a screening test and as a partial alternative to genetic tests. This is due to its low cost, straightforward routine application, moderate to high specificity, and high sensitivity.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"76 4","pages":"292-298"},"PeriodicalIF":0.8,"publicationDate":"2025-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"147576243","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases. 激素受体阳性、人类表皮生长因子受体 2 阴性、腋窝结节病负担低的早期乳腺癌患者 Ki-67 增殖指数与肿瘤型 DX 复发评分的相关性 - 137 例病例回顾。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.135859

Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili

{"title":"Correlation of Ki-67 proliferative index with oncotype DX recurrence score in hormone receptor-positive, human epidermal growth factor receptor 2-negative early breast cancer with low-burden axillary nodal disease - a review of 137 cases.","authors":"Abdalla Saad Abdalla Al-Zawi, Kristina Arsenievna Anichkina, Mohamed Elamass, Zina Aladili","doi":"10.5114/pjp.2024.135859","DOIUrl":"10.5114/pjp.2024.135859","url":null,"abstract":"The use of chemotherapy in breast cancer management has significantly contributed to the decrease in its mortality. Currently, the prognosis is determined by molecular biomarkers, such as oestrogen receptors, and human epidermal growth factor receptor 2. However, the increasing use of advanced molecular technologies, including oncotype DX recurrence score (ODX-RS), has provided the ability to estimate the risk of recurrence. Research has demonstrated that the ODX-RS helps to predict recurrence risk and the potential benefit of chemotherapy in breast cancer. As a result, it can assist clinicians in making decisions regarding using the chemotherapy. The goal of work is to explore the correlation between the ODX-RS and Ki-67 proliferative index (Ki-67-PI). This study included 137 patients with oestrogen positive, human epidermal growth factor receptor 2-negative early breast cancer, and had non- or early axillary disease. Patients with low Ki-67-PI were as follows: low ODX-RS in 17%, intermediate ODX-RS in 80%, and high ODX-RS in 2%. In the high Ki-67-PI group: low ODX-RS in 12%, intermediate ODX-RS in 48%, and high ODX-RS in 40%. In conclusion, the results show no significant correlation between the ODX-RS and Ki-67-PI (r = 0.511, p-value < 0.9).","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 1","pages":"8-18"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917322","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway. CTRP 9通过调节AMPK通路减轻OGD/R诱导的视网膜神经节细胞的凋亡和未折叠蛋白反应。

IF 0.7 4区医学

Polish Journal of Pathology Pub Date : 2024-01-01 DOI: 10.5114/pjp.2024.136025

Xiaofan Yang, Yuling Niu

{"title":"CTRP 9 mitigates the apoptosis and unfolded protein response of OGD/R-induced retinal ganglion cells by regulating the AMPK pathway.","authors":"Xiaofan Yang, Yuling Niu","doi":"10.5114/pjp.2024.136025","DOIUrl":"10.5114/pjp.2024.136025","url":null,"abstract":"C1q/TNF-related protein-9 (CTRP9) has been reported to play roles in several types of retinal diseases. However, the role and the potential mechanism of CTRP9 in glaucoma are still incompletely understood. The expression of CTRP9 in OGD/R-induced retinal ganglion cells (RGCs) was detected by quantitative real-time polymerase chain reaction and western blot assay. Cell proliferation was identified by cell counting Kit-8 assay. Flow cytometry, enzyme-linked immunosorbent assay and western blot assay were performed to assess cell apoptosis. Unfolded protein response (UPR), endoplasmic reticulum (ER) stress and the AMPK pathway were evaluated by western blot assay. The data showed that the expression of CTRP9 was significantly downregulated in OGD/R-induced 661W cells. OGD/R treatment reduced cell viability, promoted cell apoptosis and activated the UPR and ER stress. The overexpression of CTRP9 reversed the effects of OGD/R on 661W cell viability, apoptosis, the UPR and ER stress, as well as the AMPK pathway. However, Compound C, an inhibitor of AMPK signaling, reversed the protection of CTRP9 overexpression against injury from OGD/R in 661W cells. In summary, the results revealed that CTRP9 abated the apoptosis and UPR of OGD/R-induced RGCs by regulating the AMPK pathway, which may provide a promising target for the treatment of glaucoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":"75 1","pages":"40-53"},"PeriodicalIF":0.7,"publicationDate":"2024-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140917323","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0