Polish Journal of Pathology最新文献_第8页

Prognostic significance of a panel of two biomarkers (E-cadherin and CD105) in laryngeal cancer. 喉癌中两种生物标记物（E-cadherin 和 CD105）的预后意义。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.134316

Elvir Zvrko, Ljiljana Vuckovic, Miodrag Radunovic

{"title":"Prognostic significance of a panel of two biomarkers (E-cadherin and CD105) in laryngeal cancer.","authors":"Elvir Zvrko, Ljiljana Vuckovic, Miodrag Radunovic","doi":"10.5114/pjp.2023.134316","DOIUrl":"10.5114/pjp.2023.134316","url":null,"abstract":"This study aimed to evaluate the clinicopathologic significance of the combined immunohistochemical expression of the epithelial-mesenchymal transition marker E-cadherin and the angiogenesis marker CD105 in laryngeal squamous cell carcinoma and assess correlation of their expression. Eighty-five patients who underwent complete resection as primary treatment were selected for this study. E-cadherin and CD105 expression levels were determined by immunohistochemistry. The receiver operating curve approach was applied to determine the cut-off value and separate patients with high and low expression of markers. The high-risk group (\"CD105 high\" and \"E-cadherin low\" expression) showed statistically significant correlations with age less than 66 years (p = 0.039), advanced T-status (T3-4) (p = 0.046), aggressive TNM stage (stage III-IV) (p = 0.003) and locoregional recurrence of disease (p = 0.004). In the Kaplan-Meier analyses, the high-risk group had significantly worse prognoses than other risk groups (log-rank test 2 = 9.415, p = 0.024). Spearman's correlation coefficient analysis showed a nonsignificant negative correlation between the expression of E-cadherin and CD105 (rho = -0.073, p = 0.505). Simultaneous consideration of E-cadherin and CD105 is a simple panel of markers to determine aggressive tumour phenotype with a higher risk of disease recurrence in patients with laryngeal cancer.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2023-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"140112009","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Vaginal metastasis of renal clear cell carcinoma: a case report emphasizing the role of immunohistochemistry in differential diagnosis in the absence of a clinical history. 肾透明细胞癌阴道转移一例报告:强调免疫组织化学在无临床病史的情况下鉴别诊断的作用。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2023-01-01 DOI: 10.5114/pjp.2023.132229

Simona Stolnicu, Andrei Patrichi, Francisc Rozsnyai, Robert A Soslow

引用次数: 0

Laryngeal chondrosarcoma treated with conventional radiotherapy - case report and review of the literature. 常规放疗治疗喉软骨肉瘤病例报告及文献复习。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.119240

Agata Roch-Zniszczoł, Dorota Księżniak-Baran, Ewa Chmielik, Maria Goleń, Natalia Amrogowicz, Dorota Gabryś

引用次数: 0

Roles of CD3, CD4 and CD8 in synovial lymphocytes of rheumatoid arthritis. CD3、CD4和CD8在类风湿关节炎滑膜淋巴细胞中的作用。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.117173

Guangyi Xiong, Ting Lei, Shuhui Dong, Lina Xu, Mingshan Li, Ruilin Wang

{"title":"Roles of CD3, CD4 and CD8 in synovial lymphocytes of rheumatoid arthritis.","authors":"Guangyi Xiong, Ting Lei, Shuhui Dong, Lina Xu, Mingshan Li, Ruilin Wang","doi":"10.5114/pjp.2022.117173","DOIUrl":"https://doi.org/10.5114/pjp.2022.117173","url":null,"abstract":"In this study, the immunohistochemical EnVision method was applied to detect CD3, CD4 and CD8 in synovial tissues of 40 patients with rheumatoid arthritis (RA) and 10 patients with osteoarthritis (OA). In 92.5% (37/40) RA cases, lymphocytes were focally aggregated, and even germinal centers appeared, forming lymphoid follicle-like structures. The expression of CD3, CD4, and CD8 were high in synovial tissue of RA group, but low in OA group. The number of CD3, CD4+, and CD8+ lymphocytes in OA group were significantly lower than that in RA group (p < 0.05); CD4+lymphocytes in RA accounted for the majority, and mostly were focally distributed. The number of CD8+lymphocytes in the synovial tissue were small, and were mostly scattered. The number of CD4+lymphocytes were significantly higher than CD8+lymphocytes (p<0.05). Compared with the OA group, the number of CD4+T and CD8+T lymphocytes in RA group were higher, and the ratio of CD4/CD8 was higher in RA group (p < 0.05). In conclusion, the CD3, CD4 and CD8 with high level may promote the occurrence and development of RA. The ratio of CD4+/CD8+ may be used as a reference index for the diagnosis and prognosis of RA.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40515007","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 3

A novel pre-processing approach based on colour space assessment for digestive neuroendocrine tumour grading in immunohistochemical tissue images. 一种基于色彩空间评估的免疫组化组织图像中消化神经内分泌肿瘤分级的新型预处理方法。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.119841

Hana Rmili, Aymen Mouelhi, Basel Solaiman, Raoudha Doghri, Salam Labidi

{"title":"A novel pre-processing approach based on colour space assessment for digestive neuroendocrine tumour grading in immunohistochemical tissue images.","authors":"Hana Rmili, Aymen Mouelhi, Basel Solaiman, Raoudha Doghri, Salam Labidi","doi":"10.5114/pjp.2022.119841","DOIUrl":"https://doi.org/10.5114/pjp.2022.119841","url":null,"abstract":"Introduction: The complexity of histopathological images remains a challenging issue in cancer diagnosis. A pathologist analyses immunohistochemical images to detect a colour-based stain, which is brown for positive nuclei with different intensities and blue for negative nuclei. Several issues emerge during the eyeballing tissue slide analysis, such as colour variations caused by stain inhomogeneity, non-uniform illumination, irregular cell shapes, and overlapping cell nuclei. To overcome those problems, an automated computer-aided diagnosis system is proposed to segment and quantify digestive neuroendocrine tumours.Material and methods: We present a novel pre-processing approach based on colour space assessment. A criterion called pertinence degree is introduced to select the appropriate colour channel, followed by contrast enhancement. Subsequently, the adaptive local threshold technique that uses the modified Laplacian filter is applied to minimize the implementation complexity, highlight edges, and emphasize intensity variation between cells across the slide. Finally, the improved watershed algorithm based on the concave vertex graph is applied for cell separation.Results: The performance of the algorithms for nucleus segmentation is evaluated according to both the object-level and pixel-level criteria. Our approach increases segmentation accuracy, with the F1-score equal to 0.986. There is significant agreement between the applied approach and the expert's ground truth segmentation.Conclusions: The proposed method outperformed the state-of-the-art techniques based on recall, precision, the F1-score, and the Dice coefficient.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40379263","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Overexpression of kif11 is a poor prognostic factor in clear cell renal cell carcinoma. 在透明细胞肾细胞癌中，kif11的过表达是一个预后不良的因素。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.118137

Adam Kowalewski, Damian Jaworski, Paulina Antosik, Marta Smolińska, Joanna Ligmanowska, Dariusz Grzanka, Łukasz Szylberg

{"title":"Overexpression of kif11 is a poor prognostic factor in clear cell renal cell carcinoma.","authors":"Adam Kowalewski, Damian Jaworski, Paulina Antosik, Marta Smolińska, Joanna Ligmanowska, Dariusz Grzanka, Łukasz Szylberg","doi":"10.5114/pjp.2022.118137","DOIUrl":"https://doi.org/10.5114/pjp.2022.118137","url":null,"abstract":"Introduction: Unresectable renal cell carcinoma continues to be a great challenge due to our limited understanding of its underlying pathophysiology. We explored the relationship between KIF11 protein expression and the clinical courses of clear cell renal cell carcinoma (ccRCC) using a tissue microarray.Material and methods: The tissue microarray contained specimens derived from 90 patients, cancer and matched adjacent non-cancerous tissue (2 cores per case), followed up for 7 years. Tumour samples were evaluated for KIF11 expression using the H-score, and their correlations with clinicopathological data and survival data were analysed.Results: 72.7% of ccRCC tissues presented KIF11 cytoplasmic expression with a median value of 20 (interquartile range 0-200). The nuclear staining was positive in 36.36% of ccRCC tissues. Among controls, nuclear KIF11 expression was absent, but cytoplasmic expression was identified in all cases, with a median value of 230 (interquartile range 45-290). Cytoplasmic KIF11 expression in ccRCC tissues was lower than in the control tissues and was positively correlated with tumour grade and mortality (p < 0.05). KIF11 nuclear expression did not correlate with overall survival.Conclusions: Elevated expression of KIF11 predicts poor clinical outcome in ccRCC patients. Downregulation of KIF11 may provide a new therapeutic strategy for ccRCC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40423172","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical clinical presentation mimicking stroke in an adult patient caused by a rare diffuse leptomeningeal glioneuronal tumour. 一个罕见的弥漫性脑脊膜神经胶质细胞肿瘤引起的非典型临床表现。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125822

Angelika Stapińska-Syniec, Anna Gogol, Albert Acewicz, Michał Sobstyl, Teresa Wierzba-Bobrowicz, Wiesława Grajkowska

引用次数: 1

Expressions of EZH2 and NOTCH3 pathway in osteosarcoma and their roles in osteosarcoma stem cells. EZH2和NOTCH3通路在骨肉瘤中的表达及其在骨肉瘤干细胞中的作用

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125534

Guangning Yan, Yangfan Lv, Xuwen Lai, Zhenlian Li, Danli Ye, Wei Wang, Yunfeng Qian, Haojie Yu, Zhuocai Wang

{"title":"Expressions of EZH2 and NOTCH3 pathway in osteosarcoma and their roles in osteosarcoma stem cells.","authors":"Guangning Yan, Yangfan Lv, Xuwen Lai, Zhenlian Li, Danli Ye, Wei Wang, Yunfeng Qian, Haojie Yu, Zhuocai Wang","doi":"10.5114/pjp.2022.125534","DOIUrl":"https://doi.org/10.5114/pjp.2022.125534","url":null,"abstract":"Osteosarcoma (OS) is the most common malignant bone tumour; however, the underlying mechanisms are mainly unknown. Enhancer of zeste homologue 2 (EZH2) and NOTCH pathway are important molecular signals related to carcinogenesis and tumour progression, but they are not fully understand in OS. Enhancer of zeste homologue 2, Notch3, HES1, and Nanog were detected on OS samples and statistically analysed. Expressions of these genes were investigate, and stem-like phenotype was verified in OS cells. This study found that higher EZH2 expression, Notch3 pathway, or Nanog were associated with tumour relapse and metastasis and a significantly shorter survival time. Moreover, the Notch3 pathway was activated in osteosarcoma stem cells. Enhancer of zeste homologue 2 overexpression could activate the Notch3 pathway and increase HES1 expression, leading to upregulated stem cell-related gene expression and self-renewal of OS cells. Our study demonstrates that EZH2, Notch3, and Nanog are important prognostic factors. Enhancer of zeste homologue 2 could maintain the self-renewal of OS cells, where the Notch3 pathway activation may be involved.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9593349","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Optimal DNA quality preservation process for comprehensive genomic testing of pediatric clinical autopsy formalin-fixed, paraffin-embedded tissues. 用于儿科临床尸检福尔马林固定石蜡包埋组织的全面基因组检测的最佳DNA质量保存工艺。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.124492

Noriko Watanabe, Tomohiro Umezu, Masashi Kyushiki, Kayoko Ichimura, Atsuko Nakazawa, Masahiko Kuroda

{"title":"Optimal DNA quality preservation process for comprehensive genomic testing of pediatric clinical autopsy formalin-fixed, paraffin-embedded tissues.","authors":"Noriko Watanabe, Tomohiro Umezu, Masashi Kyushiki, Kayoko Ichimura, Atsuko Nakazawa, Masahiko Kuroda","doi":"10.5114/pjp.2022.124492","DOIUrl":"https://doi.org/10.5114/pjp.2022.124492","url":null,"abstract":"Clinical autopsies are performed to reveal the process of the disease that caused patient death and validate the diagnosis and treatment decisions. In pediatric clinical autopsy, the feedback provided to bereaved families has a considerable social impact; however, pediatric diseases are diverse, which makes it difficult to elucidate them. Therefore, it is necessary to employ molecular biology techniques in addition to conventional methods. Formalin-fixed, paraffin-embedded (FFPE) tissues are routinely prepared. However, clinical autopsy FFPE tissue processing is not standardized, and it is unclear whether DNA from such tissues can be used for comprehensive genomic analysis. In this study, we evaluated the DNA quality of FFPE tissues from 15 recent autopsy cases at a single-center children's hospital using quantitative polymerase chain reaction [PCR (Q129/Q41)] and nanoelectrophoresis (DNA integrity number (DIN)). Good quality DNA was obtained from every organ type excluding bone marrow within 6 days of formalin fixation. Prolonged proteinase K digestion (48 h > 24 h > 1 h) and thicker tissue sections (10 µm > 1 µm) improved Q129/Q41; however, 24 h fixed FFPE tissues showed better DNA quality. We propose an optimal and feasible workflow for storing short-term fixed FFPE tissues as DNA-preserved FFPE tissues for future comprehensive genomic searches.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10652767","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The histopathologic checkpoints for thyroid core needle biopsy compared with resection sections. 甲状腺核心穿刺活检与切除切片的组织病理学检查点比较。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125778

Tugce Kiran, Beril Guler

{"title":"The histopathologic checkpoints for thyroid core needle biopsy compared with resection sections.","authors":"Tugce Kiran, Beril Guler","doi":"10.5114/pjp.2022.125778","DOIUrl":"https://doi.org/10.5114/pjp.2022.125778","url":null,"abstract":"Introduction: The nuclear features that are thought to be classic indicators of thyroid nodules are not fully observed in core needle biopsies (CNB). The aim of this study was to evaluate the histopathological differences between CNB samples and resection sections.Material and methods: The haematoxylin and eosin-stained CNB and resection sections of thyroid nodules were retrospectively re-evaluated in terms of nuclear and architectural parameters. The evaluations were conducted by 2 pathologists. Statistical analysis was applied in 80 selected cases diagnosed as benign (n = 37) and papillary carcinoma/suspicious for papillary carcinoma (n = 43).Results: The nuclear findings in the CNB were more subtle than in the resection sections. The nuclei were smaller (measurements of the nuclear areas, major axes, and minor axes in CNB and resection sections were 52.62 µm2, 9.89 µm, 6.75 µm, 129.18 µm2, 14.53 µm, and 10.79 µm, respectively). Hypochromia was detected in 46.5% of the papillary carcinoma cases. Grooves and pseudoinclusions were the other nuclear features that could be detected. However, nuclear contour irregularity was the most reliable finding that could predict papillary carcinoma diagnosis in the CNB sections (v: 0.82, p < 0.001).Conclusions: We believe that the histopathological differences we found have an important place in diagnostics and should be emphasized, and new diagnostic algorithms should be developed.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9158599","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0