Polish Journal of Pathology最新文献_第9页

Clinical significance and effect of PRR 11 up-regulation on the malignancy of osteosarcoma. prr11上调对骨肉瘤恶性肿瘤的临床意义及影响。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.124493

Rui Tan, Chunbing Zhao, Yangcheng Lu, Lei Chen

{"title":"Clinical significance and effect of PRR 11 up-regulation on the malignancy of osteosarcoma.","authors":"Rui Tan, Chunbing Zhao, Yangcheng Lu, Lei Chen","doi":"10.5114/pjp.2022.124493","DOIUrl":"https://doi.org/10.5114/pjp.2022.124493","url":null,"abstract":"Osteosarcoma ranks first in both morbidity and mortality among primary bone tumors. This study aimed to investigate the effect of up-regulation of PRR11 on the malignancy of osteosarcoma and its clinical significance. The expression, biological function, related pathways of PRR11 in osteosarcoma and its impact on prognosis were explored through the bioinformatics database. After PRR11 was up-regulated, cell proliferation, invasion, migration and apoptosis were detected by the cell counting kit-8 method, Transwell, scratch, and flow cytometry. PRR11 is highly expressed in a variety of malignant tumors, including osteosarcoma tissue and cells, and has a significant impact on prognosis. Univariate Cox regression analysis revealed that PRR11 was an independent prognostic factor for osteosarcoma. The gene set enrichment analysis results showed that the differential genes were mainly enriched in the biological process of the cell cycle; the protein-protein interaction network mainly interacted with the regulatory genes of the cell cycle. PRR11 promotes the invasion, migration, and proliferation of osteosarcoma cells and inhibits their apoptosis. Comprehensive bioinformatics analysis revealed that PRR11 promotes the malignancy of osteosarcoma cells mainly by participating in cell cycle regulation, and has an important impact on osteosarcoma prognosis. PRR11 may provide the basis for prognosis and treatment in patients with osteosarcoma.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10665899","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Placental recent/on-going foetal vascular malperfusion with endothelial fragmentation is diagnostically equivalent to established distal villous lesions of foetal vascular malperfusion. 胎盘近期/持续的胎儿血管灌注不良伴内皮破裂在诊断上等同于胎儿血管灌注不良的远端绒毛病变。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.124487

Jerzy Stanek

{"title":"Placental recent/on-going foetal vascular malperfusion with endothelial fragmentation is diagnostically equivalent to established distal villous lesions of foetal vascular malperfusion.","authors":"Jerzy Stanek","doi":"10.5114/pjp.2022.124487","DOIUrl":"https://doi.org/10.5114/pjp.2022.124487","url":null,"abstract":"CD34 immunostaining increases the sensitivity of placental diagnosis of foetal vascular malperfusion (FVM). This comparative retrospective study was performed to find out whether recent distal FVM lesions diagnosed with CD34 are diagnostically equivalent to remote FVM lesions diagnosed with haematoxylin-eosin (H&E). Clinical and placental phenotypes of 562 placentas from ≥ 20-week, high-risk pregnancies were analysed: Group 1-158 placentas with remote distal villous FVM (by H&E only), Group 2-142 placentas showing clustered endothelial fragmentation by CD34 immunostaining, 98 of them also with H&E distal FVM lesions (on-going, temporal heterogeneity), and Group 3-262 placentas without distal villous FVM. In Group 1, gestational age was the shortest, postnatal mortality most frequent, placental weight the smallest, and intra villous haemorrhage, erythroblasts in foetal blood, hypertrophic decidual arteriopathy, and foetal vascular thrombi most common. In Group 2, placental infarction, post-uterine pattern of chronic placental injury, and excessive extra villous trophoblasts of chorionic disc were most common (p < 0.05). In this cohort of foetuses/neonates dominated by congenital malformations, distal villous FVM was the most common pattern of placental injury, and those diagnosed by CD34 and by H&E are diagnostically/prognostically equivalent. CD34 immunostaining is therefore a powerful tool in the diagnosis of distal villous FVM.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10708152","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

Immunohistochemical expression of PARP-1 in triple-negative endometrial cancer - a comparison of different score systems. PARP-1在三阴性子宫内膜癌中的免疫组织化学表达——不同评分系统的比较

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125613

Michał Bulsa, Sylwia Romana Jankowska-Szabłowska, Elżbieta Urasińska

{"title":"Immunohistochemical expression of PARP-1 in triple-negative endometrial cancer - a comparison of different score systems.","authors":"Michał Bulsa, Sylwia Romana Jankowska-Szabłowska, Elżbieta Urasińska","doi":"10.5114/pjp.2022.125613","DOIUrl":"https://doi.org/10.5114/pjp.2022.125613","url":null,"abstract":"Endometrial cancer is the most common malignant neoplasm of the female reproductive system. The number of diagnosed cases is increasing every year. In recent years, the triple-negative phenomenon (TNP) has been identified as one of the determinants of shorter survival in endometrial cancer patients. The aim of the study was to compare the PARP-1 protein expression in triple-negative (TNEC) and non-triple-negative (NTNEC) endometrial cancer patients and determine the relationship between the PARP-1 protein expression in endometrial cancer cells and patient's overall survival depending on the adopted scale (H-SCORE < 75, H-SCORE < 50, Allred scale). The study involved 265 patients with histopathologically confirmed endometrial cancer. The patients were divided into 2 groups: patients with TNEC and patients with NTNEC. The study was conducted using a tissue microarray technique. Expression of PARP-1 protein was determined by immunohistochemistry. Protein expression evaluation was performed using virtual microscopy and the Image Scope computer image analysis system. The following conclusions were reached: total and individual levels of nuclear or cytoplasmic PARP-1 expression varied with the presence or absence of TNP, and PARP-1 nuclear expression at the 2+ level had a significant effect on the increased risk of death (according to H-SCORE < 75).","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9158601","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

LncRNA FGD5-AS1 drives the malignant development of gastric cancer by negatively interacting with FZD3. LncRNA FGD5-AS1通过与FZD3负相互作用驱动胃癌的恶性发展。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.117179

Limin Feng, Hui Zheng, Huaping Zhang, Mingxiao Cao, Hua Zhou

{"title":"LncRNA FGD5-AS1 drives the malignant development of gastric cancer by negatively interacting with FZD3.","authors":"Limin Feng, Hui Zheng, Huaping Zhang, Mingxiao Cao, Hua Zhou","doi":"10.5114/pjp.2022.117179","DOIUrl":"https://doi.org/10.5114/pjp.2022.117179","url":null,"abstract":"We aimed to detect the expression pattern of long non-coding RNA (lncRNA) FGD5-AS1 in gastric cancer (GC) samples and its impact on driving the development of GC. FGD5-AS1 levels in 66 cases of GC tissues and paracancerous ones were detected. Its influences on clinical features and prognosis in GC patients were analyzed. In AGS and SGC-7901 cells with FGD5-AS1 knockdown, phenotype changes were assessed through cell counting kit-8 (CCK-8), Transwell and wound healing assay. The downstream target of FGD5-AS1 was searched by a bioinformatics tool and confirmed by dual-luciferase reporter assay. Their interaction in regulating the malignant development of GC was finally explored. FGD5-AS1 was upregulated in GC tissues compared to paracancerous ones. GC patients expressing a high level of FGD5-AS1 had higher risk of lymphatic metastasis or distant metastasis and worse prognosis than those with a low level. Knockdown of FGD5-AS1 weakened proliferative and metastatic abilities in AGS and SGC-7901 cells. FZD3 was the downstream target of FGD5-AS1. Protein levels of FZD3 and FZD5 were upregulated, while b-catenin, TGF-b and MMP9 were downregulated in GC cells with FGD5-AS1 knockdown. Knockdown of FZD3 abolished the regulatory effects of FGD5-AS1 on malignant phenotypes of GC cells. FGD5-AS1 is upregulated in GC samples, which is linked to metastasis and prognosis in GC. It drives proliferative and metastatic abilities in GC cells via negatively interacting with FZD3.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"40515960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The rare combination of low-grade thyroid-like nasopharyngeal papillary adenocarcinoma and squamous cell carcinoma: a case report and literature review. 罕见的低级别甲状腺样鼻咽乳头状腺癌合并鳞状细胞癌1例报告并文献复习。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125569

Tugba Toyran, Merve Inceman, Kıvılcım Erdogan, Aysegül Kücükosmanoglu, Nur Yücel Ekici, Aysun Hatice Ugu

引用次数: 0

Evaluation of pd-1, pd-l1, and cytotoxic T-lymphocyteassociated protein 4 expressions together with clinicopathological findings in clear cell, papillary, and chromophobe types of renal cell carcinoma. 透明细胞型、乳头状型和憎色型肾细胞癌中pd-1、pd-l1和细胞毒性t淋巴细胞相关蛋白4表达及临床病理结果的评估

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.122613

Merve Inceman, Tugba Toyran, Yildirim Bayazit, Volkan Izol, Seyda Erdogan

{"title":"Evaluation of pd-1, pd-l1, and cytotoxic T-lymphocyteassociated protein 4 expressions together with clinicopathological findings in clear cell, papillary, and chromophobe types of renal cell carcinoma.","authors":"Merve Inceman, Tugba Toyran, Yildirim Bayazit, Volkan Izol, Seyda Erdogan","doi":"10.5114/pjp.2022.122613","DOIUrl":"https://doi.org/10.5114/pjp.2022.122613","url":null,"abstract":"Our study aimed to determine the expressions of programmed death protein 1 (PD-1), programmed death ligand protein 1 (PD-L1), and cytotoxic T-lymphocyte- associated protein 4 (CTLA-4) to investigate and compare the differences between early and advanced cases in the 3 most common types of renal cell carcinoma (RCC) and reveal their correlations with prognosis and survival. A total of 166 RCC cases diagnosed between 2010 and 2019 in our hospital were included. PD-1, PD-L1, and CTLA-4 markers were applied to the paraffin blocks of the cases using an immunohistochemical method, and their expression status was evaluated by distinguishing subtypes in advanced- and early-stage RCCs. It was observed that PD-L1 positivity in the tumour cells, in clear cell RCC, was statistically significantly more frequent in advanced-stage cases compared to early-stage cases. It was concluded that cases with PD-L1 positivity in tumour- infiltrating mononuclear cells (TIMC) in clear cell and chromophobe RCC had a shorter survival. The frequency of perinephritic fat invasion and necrosis was higher in cases with PD-L1 expression in TIMC. We think that PD-1, PD-L1, and CTLA-4 must be considered together in advanced stage RCC for the treatment of both pathway inhibitors. Further large studies will shed light on the immunotherapy options at the advanced stage of all RCC types even in the absence of metastasis.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10707678","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Expression of fascin in association with p16 and Ki-67 in cervical lesions: immunohistochemical study. 免疫组化研究筋膜蛋白与p16和Ki-67在宫颈病变中的表达。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.124488

Sandra Csizmár, Desanka Výbohová, Veronika Mešt'anová, Bibiána Krajňáková, Karol Kajo, Lenka Kunertová, Marian Adamkov

{"title":"Expression of fascin in association with p16 and Ki-67 in cervical lesions: immunohistochemical study.","authors":"Sandra Csizmár, Desanka Výbohová, Veronika Mešt'anová, Bibiána Krajňáková, Karol Kajo, Lenka Kunertová, Marian Adamkov","doi":"10.5114/pjp.2022.124488","DOIUrl":"https://doi.org/10.5114/pjp.2022.124488","url":null,"abstract":"Annual gynecological examination with cervical cancer screening and HPV vaccination ensures the appropriate prevention of the onset and progression of cervical cancer. Currently, efforts are being made to find new diagnostic and prognostic biomarkers. Fascin, an actin-bundling protein, promotes cellular migration. Its overexpression has been observed in many types of squamous carcinomas and was usually correlated with a worse prognosis and metastasis. However, the data on fascin expression in cervical lesions are limited. This study focuses on the quantitative evaluation of fascin expression, the immunoreaction intensity and subcellular localization of fascin expression in low-grade squamous intraepithelial lesions (LSIL), high-grade squamous intraepithelial lesions (HSIL) and squamous cell carcinomas (SCC). Fascin expression was also correlated with the routinely used diagnostic markers p16 and Ki-67. Biopsy specimens (n = 67) of LSIL, HSIL and SCC were taken from adult women in the age range 20-86 years. Fascin expression was detected by immunohistochemical analysis and quantified using morphometric software. Analysis of variance confirmed statistically significant differences in the percentage of fascin-positive cells between the LSIL, HSIL and SCC groups. Finally, the results showed a significant positive correlation between fascin expression and p16 and Ki-67 expression.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10708153","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 1

The relationship of tumour-associated macrophages (CD68, CD163, CD11c) and cancer stem cell (CD44) markers with prognostic parameters in breast carcinomas. 肿瘤相关巨噬细胞(CD68、CD163、CD11c)和肿瘤干细胞(CD44)标志物与乳腺癌预后参数的关系

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125424

Suna Sari, Çiğdem Özdemir, Murat Çilekar

{"title":"The relationship of tumour-associated macrophages (CD68, CD163, CD11c) and cancer stem cell (CD44) markers with prognostic parameters in breast carcinomas.","authors":"Suna Sari, Çiğdem Özdemir, Murat Çilekar","doi":"10.5114/pjp.2022.125424","DOIUrl":"https://doi.org/10.5114/pjp.2022.125424","url":null,"abstract":"Introduction: In invasive breast cancer (IBC), tumour-associated macrophages and cancer stem cells in the tumour microenvironment are thought to be associated with poor prognosis, tumour growth, and metastasis.Material and methods: In this study, we evaluated the relationship of CD68, CD163, CD11c, and CD44 expressions in the tumour microenvironment of IBC with prognostic parameters by immunohistochemical method.Results: A correlation was found between high histological grade, oestrogen receptor/ progesterone receptor negativity, and high Ki-67 index in IBC, and the number of CD68-, CD163-, and CD11c-positive macrophages in the tumour microenvironment. In addition, in the tumour microenvironment, a correlation was found between IBC-specific subtypes and CD68, luminal A subtype relative to luminal B and CD163, HER2 score 3 cases, and CD11c-positive macrophage count. It was observed that CD44 expression in the tumour in IBC was not associated with the parameters examined. In addition, a high-level linear correlation was found between CD68- and CD163- and CD11c-positive macrophage counts. Tumour-associated macrophages in the tumour microenvironment in IBC may be a novel prognostic factor and an alternative avenue for targeted therapies.Conclusions: The fact that CD44 positivity in the tumour is not associated with prognostic parameters will contribute to the literature in this direction.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"9526885","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

The significance of StAR-related lipid transfer protein-3 expression in breast cancer. star相关脂质转移蛋白-3在乳腺癌中的表达意义

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.124489

Abdulfattah Fararjeh, Ezidin Kaddumi, Ali Al-Khader, Walid Aburayyan

{"title":"The significance of StAR-related lipid transfer protein-3 expression in breast cancer.","authors":"Abdulfattah Fararjeh, Ezidin Kaddumi, Ali Al-Khader, Walid Aburayyan","doi":"10.5114/pjp.2022.124489","DOIUrl":"https://doi.org/10.5114/pjp.2022.124489","url":null,"abstract":"StAR-related lipid transfer domain containing proteins (STARD3) are a group of proteins that contain a steroidogenic acute regulatory protein-related lipid transfer domain. Breast cancer (BC) has been linked to the STARD3 gene. In this study, we sought to confirm the relationship of STARD3 protein expression with clinicopathological characteristics and BC molecular subtypes. Using tissue microarray, we examined the STARD3 protein expression in 200 BC tissues and 20 normal breast tissues. Higher protein expression of STARD3 was found in tumor tissues than normal tissues. One hundred and fifty-two (69.1%) of the 200 cases tested positive for STARD3 (high H-score), while seventy (30.9%) had a low STARD3 H-score. When STARD3 is present, its expression ranges from mild to strong. STARD3 H-score was strongly linked to human epidermal growth factor receptor 2 (HER2)-positive (p < 0.001) and estrogen receptor (ER)-positive (p < 0.009) patients, but not to triple-negative BC patients. STARD3 high H-score was associated with histological grade and tumor size. No significant associations were found with stage of the disease, pathological stage or node status. Our research revealed that STARD3 levels were higher in tissues from malignant BC, and it was associated with HER2 and ER, suggesting that it might be utilized as a marker for BC.","PeriodicalId":49692,"journal":{"name":"Polish Journal of Pathology","volume":null,"pages":null},"PeriodicalIF":0.8,"publicationDate":"2022-01-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"10660113","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":4,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

引用次数: 0

Atypical spindle cell lipomatous tumour presenting as a large retroperitoneal mass - a case report and review of the literature. 非典型梭形细胞脂肪瘤表现为腹膜后大肿块- 1例报告及文献复习。

IF 0.8 4区医学

Polish Journal of Pathology Pub Date : 2022-01-01 DOI: 10.5114/pjp.2022.125654

Martyna Krupinska, Carmen León, Rocío López Henríquez, Beatriz Esquivel Vázquez, Rosa Nieves Rodríguez Rodríguez, Patricia Marrero Marrero, Luciano Delgado Plasencia, Eduardo Salido

引用次数: 0