Effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells.

IF 0.7 4区医学 Q4 PATHOLOGY

Polish Journal of Pathology Pub Date : 2023-01-01 DOI:10.5114/pjp.2022.119264

Min Yuan, Chengyu Li, Xiang Xiao, Dengfeng Wan, Bin Xi, Xingxing Jiang, Jianzhong Zhang

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引用次数: 1

Abstract

Aim of the study: Aim of the study is To investigate the effect of lentinan on proliferation and apoptosis of human astrocytoma U251 cells. Lentinan was dissolved in DMEM complete medium to form different concentrations (0, 25, 50, 100, 200, 400, 500, 600 µg/ml). CCK8 was used to detect the effect of lentinan with different concentrations on proliferation of human astrocytoma U251 cells, and the expression of Ki-67 was detected by immunofluorescence. In addition, the effect of different concentrations of lentinan on apoptosis of human astrocytoma U251 cells was detected by flow cytometry. Compared with the blank control group, 50 and 100 µg/ml lentinan significantly promoted proliferation of human astrocytoma U251 cells. When the concentration is more than 100 µg/ml, the cell activity gradually decreases, and the cell activity is the lowest when the concentration is 600 µg/ml. In addition, the low concentration lentinan (25, 50, and 100 µg/ml) had no significant effect on apoptosis of human astrocytoma U251 cells. However, lentinan above 200 µg/ml significantly promoted apoptosis of human astrocytoma U251 cells and had a concentration gradient effect, and the highest apoptosis rate was at 600 µg/ml.

Conclusions: Lentinan can effectively inhibit proliferation and promote apoptosis of human astrocytoma U251 cells.

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香菇多糖对人星形细胞瘤U251细胞增殖和凋亡的影响。

研究目的：研究香菇多糖对人星形细胞瘤U251细胞增殖和凋亡的影响。将香菇多糖溶解在DMEM完全培养基中以形成不同浓度（0、25、50、100、200、400、500、600µg/ml）。用CCK8检测不同浓度香菇多糖对人星形细胞瘤U251细胞增殖的影响，并用免疫荧光法检测Ki-67的表达。此外，通过流式细胞术检测不同浓度的香菇多糖对人星形细胞瘤U251细胞凋亡的影响。与空白对照组相比，50和100µg/ml香菇多糖显著促进人星形细胞瘤U251细胞的增殖。当浓度超过100µg/ml时，细胞活性逐渐降低，当浓度为600µg/ml时细胞活性最低。此外，低浓度的香菇多糖（25、50和100µg/ml）对人星形细胞瘤U251细胞的凋亡没有显著影响。然而，200µg/ml以上的香菇多糖显著促进人星形细胞瘤U251细胞凋亡，并具有浓度梯度效应，最高凋亡率为600µg/ml。结论：香菇多糖能有效抑制人星形细胞癌U251细胞增殖，促进细胞凋亡。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Polish Journal of Pathology PATHOLOGY-

CiteScore

1.00

自引率

0.00%

发文量

审稿时长

>12 weeks

期刊介绍： Polish Journal of Pathology is an official magazine of the Polish Association of Pathologists and the Polish Branch of the International Academy of Pathology. For the last 18 years of its presence on the market it has published more than 360 original papers and scientific reports, often quoted in reviewed foreign magazines. A new extended Scientific Board of the quarterly magazine comprises people with recognised achievements in pathomorphology and biology, including molecular biology and cytogenetics, as well as clinical oncology. Polish scientists who are working abroad and are international authorities have also been invited. Apart from presenting scientific reports, the magazine will also play a didactic and training role.