Ultrasound in Medicine and Biology最新文献

{"title":"Ultrasound Assessment of Fluid Tolerance in Critically Ill Patients: Epidemiology of Systemic and Pulmonary Congestion","authors":"Peter Klompmaker ,&nbsp;Amne Mousa ,&nbsp;Dylan J. Allard ,&nbsp;Bram S.H. Hagen ,&nbsp;Tessa Bánki ,&nbsp;Judith van Zwol ,&nbsp;Krijna Opschoor ,&nbsp;Carolien A. den Otter ,&nbsp;Thomas P. Ootjers ,&nbsp;Alexander P.J. Vlaar ,&nbsp;Denise P. Veelo ,&nbsp;Pieter R. Tuinman","doi":"10.1016/j.ultrasmedbio.2025.11.003","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.003","url":null,"abstract":"<div><h3>Objective</h3><div>By using lung ultrasound and Venous Excess Ultrasound Grading System (VExUS) to assess fluid tolerance, four congestion types can be identified: fluid tolerant (no congestion), pulmonary congestion, systemic congestion and both pulmonary and systemic congestion. The primary aim is to describe the epidemiology of these congestions types.</div></div><div><h3>Methods</h3><div>This is a pre-planned secondary analysis of a prospective observational cohort study. Fluid tolerance was defined as absence of congestion on ultrasound. Pulmonary congestion was defined as a bilateral B-profile. Systemic congestion was defined as VExUS ≥ 2, and pulmonary and systemic congestion were used when both were present. Primary outcome was the prevalence of congestion types. Secondary outcomes were associations between congestion types, associations with left (LVF) and right ventricular function (RVF) and cumulative fluid balance.</div></div><div><h3>Results</h3><div>A total of 87 (63%) of 138 patients were fluid tolerant, 36 (27%) had pulmonary congestion, 8 (6%) systemic congestion and 7 (5%) both pulmonary and systemic congestion. Between the first and second measurement 16 out of 87 (18%, 95% CI: 11%–28%) fluid tolerant patients developed pulmonary congestion and 8 out of 36 (22%, 95% CI: 11%–40%) patients with pulmonary congestion switched to fluid tolerance. No association was found between pulmonary and systemic congestion. A positive association was found for reduced LVF and pulmonary congestion (OR 2.1 [95% CI: 1.03–4.6]), and reduced RVF and systemic congestion OR 4.3 (95% CI: 1.5–15.6). No association between cumulative fluid balance and any type of congestion was found.</div></div><div><h3>Conclusion</h3><div>Most critically ill patients are fluid tolerant at admission. Pulmonary congestion is the most prevalent congestion type. Pulmonary and systemic congestion seldom occur simultaneously; therefore, both need to be assessed.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 596-601"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145662456","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reframing CEUS-LI-RADS Through Mechanistic and Translational Lenses: Reflections on Machine Learning–Augmented Diagnosis of Hepatocellular Carcinoma","authors":"Nav La ,&nbsp;Schawanya K. Rattanapitoon ,&nbsp;Chutharat Thanchonnang ,&nbsp;Nathkapach K. Rattanapitoon","doi":"10.1016/j.ultrasmedbio.2025.11.664","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.664","url":null,"abstract":"","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Page 725"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146049239","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Evaluating the Role of Point-of-Care Ultrasound in Central Venous Pressure Monitoring for Critically Ill Patients. A Comprehensive Systematic Review and Meta-analysis","authors":"Eman E. Shaban ,&nbsp;Yavuz Yigit ,&nbsp;Benny Ponappan ,&nbsp;Ahmed Shaban ,&nbsp;Amira Shaban ,&nbsp;Mohamed Helmi Ahmed ,&nbsp;Yasser Osman Abdelaal ,&nbsp;Hany A. Zaki","doi":"10.1016/j.ultrasmedbio.2025.04.008","DOIUrl":"10.1016/j.ultrasmedbio.2025.04.008","url":null,"abstract":"<div><div>Central venous pressure (CVP) is a critical parameter for assessing volume status in critically ill patients, traditionally measured through invasive central venous catheterization. While effective, this method poses risks such as infection and procedural complications. Point-of-care ultrasound (POCUS) has emerged as a promising, non-invasive alternative, yet its accuracy in estimating CVP remains debated.</div><div>From inception to December 2024, PubMed, Embase, MEDLINE, Web of Science, and Google Scholar databases were extensively searched. The search strategy used to identify relevant studies from these databases involved combining keywords such as “Point-of-care ultrasound,” “central venous pressure,” and “critically ill” with Boolean expression “AND” and “OR.” Two independent reviewers then screened all potential studies and included those reporting the accuracy of POCUS in predicting CVP and those reporting the correlation between POCUS-measured indices and invasively-measured CVP in critically ill patients. Additionally, two independent reviewers extracted the relevant data from the included studies. Statistical analyses were conducted using MedCalc and Meta-Disc software, and quality appraisal was assessed using the QUADAS-2 tool.</div><div>The review included studies evaluating POCUS-derived indices from the inferior vena cava (IVC) and internal jugular vein (IJV). Overall, strong correlations were observed between POCUS measurements and CVP, with IVC parameters showing the strongest associations. IJV measurements also demonstrated moderate reliability, particularly in scenarios where IVC assessments were impractical. Sensitivity and specificity analyses indicated that POCUS-derived indices could predict CVP with variable accuracy.</div><div>These findings support the use of POCUS as a practical, non-invasive tool for estimating CVP in critically ill patients. IVC measurements appear to provide the most reliable correlations, while IJV assessments serve as a useful alternative. Despite its potential, variability in study methodologies and patient factors highlights the need for further research to refine POCUS-based CVP estimation and improve clinical application.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 473-484"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"144129111","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Acoustic Activation Imaging of Intravenous Perfluoropropane Droplets To Detect Risk Area and Ultimate Infarct Size in a Large Animal Model of Ischemia/Reperfusion","authors":"Shouqiang Li ,&nbsp;Cheng Chen ,&nbsp;John Lof ,&nbsp;Elizabeth A. Stolze ,&nbsp;Jiri Sklenar ,&nbsp;Xucai Chen ,&nbsp;John J. Pacella ,&nbsp;Flordeliza S. Villanueva ,&nbsp;Terry O. Matsunaga ,&nbsp;E. Carr Everbach ,&nbsp;Stanley F.J. Radio ,&nbsp;Sruti Shiva ,&nbsp;Chad Hovseth ,&nbsp;Feng Xie ,&nbsp;Xiaoping Leng ,&nbsp;Thomas R. Porter","doi":"10.1016/j.ultrasmedbio.2025.10.016","DOIUrl":"10.1016/j.ultrasmedbio.2025.10.016","url":null,"abstract":"<div><h3>Objective</h3><div>Acoustically activatable perfluoropropane droplets (PD) have been utilized to enhance the reperfused zone in acute myocardial infarction small animal models. Despite the correlation with infarct size, the enhanced area consistently overestimates infarct size. We hypothesized that the enhanced area correlates with risk area, and that hypo-enhanced areas (HEA) within the enhanced zone represent the ultimate infarct size.</div></div><div><h3>Methods</h3><div>Intravenous PD was administered in the reperfusion period following a 90 min period of ischemia in 30 pigs. Injections were given at either 30 min, 1 d or 7 d following reperfusion to determine what the enhanced regions within the reperfused zone following acoustic activation (AA) were detecting. Correlations of the enhanced and unenhanced regions with the infarct size using delayed enhancement magnetic resonance imaging (DE-MRI) were determined.</div></div><div><h3>Results</h3><div>AA within the reperfused zone was evident in 26 pigs (9/10 pigs injected at 30 min post-reperfusion, 9/10 pigs injected at 1 d and 8/10 pigs injected at 7 d). In the 26 pigs with AA, the extent of enhancement was significantly larger than the infarct size by DE-MRI, and correlated more closely with risk area (<em>r</em> = 0.55, <em>p</em> = 0.0002). A HEA within the enhanced region was observed in 21 of the 26 pigs with AA, and the spatial extent of HEA correlated with infarct size (<em>r</em> = 0.85; <em>p</em> &lt; 0.0001).</div></div><div><h3>Conclusion</h3><div>AA of intravenous PD enhances the risk area following reperfusion. However, it is the extent of hypo-enhancement within the enhanced region that predicts infarct size irrespective of the time AA is performed following reperfusion.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 531-541"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145847066","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Resting-state Functional Ultrasound Imaging of Cerebral Blood Flow and Functional Connectivity Changes in Hibernating Mice","authors":"Yanjun Zhang ,&nbsp;Yijun Zhou ,&nbsp;Yang Liu ,&nbsp;Yongchao Wang ,&nbsp;Lihai Fan ,&nbsp;Kun Song ,&nbsp;Jianbo Tang","doi":"10.1016/j.ultrasmedbio.2025.11.660","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.660","url":null,"abstract":"<div><div>Hibernation can significantly reduce energy consumption and prolong survival by decreasing the metabolic rate and body temperature. Understanding the brain regulatory mechanisms may help overcome challenges in applying hibernation techniques to humans. In this study, we induced hibernation-like state in mice using pharmacological agents and monitored the process within the brain using resting-state functional ultrasound imaging. During hibernation and recovery, we measured various physiological parameters, including heart rate, body temperature, and cerebral blood volume. Additionally, we observed cerebral hemodynamic metrics and brain functional connectivity (FC) patterns. The results revealed significant reductions in heart rate, body temperature, and cerebral blood volume during this state. Notably, cerebral blood flow (CBF) decreased by about 50% on average, with the thalamus showing a more pronounced and delayed decline compared to other examined regions, accompanied by corresponding alterations in FC. A strong correlation (<em>r</em> = 0.82) was observed between changes in CBF and brain FC. This study provides insights into the unique regulation of CBF during hibernation, offering a deeper understanding of the brain regulation in this process.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 665-672"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145829010","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sensitivity to Detail: A Meta-Analytic Essential — Especially When It’s Missing","authors":"Javier Arredondo Montero MD, PhD","doi":"10.1016/j.ultrasmedbio.2025.06.003","DOIUrl":"10.1016/j.ultrasmedbio.2025.06.003","url":null,"abstract":"","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 721-722"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"146048955","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Use of Diagnostic Ultrasound for the Assessment of Rotator Cuff Tendinopathy: A Systematic Review","authors":"Gabriella Gould ,&nbsp;Chloe Macindoe ,&nbsp;Joshua Wong ,&nbsp;Wesley Matthews","doi":"10.1016/j.ultrasmedbio.2025.10.241","DOIUrl":"10.1016/j.ultrasmedbio.2025.10.241","url":null,"abstract":"<div><h3>Background</h3><div>Rotator cuff tendinopathy (RCT) is a common clinical diagnosis of pain and dysfunction in the shoulder. In conjunction with clinical tests, diagnostic ultrasound (US) is beneficial in the diagnosis of RCT through its ability to visualise tendon structural change. However, there is currently no standardised method to assess RCT with US.</div></div><div><h3>Aims/Purpose</h3><div>The primary aim of this systematic review was to identify the common US parameters used to diagnose RCT with US. The secondary aim was to determine whether a nominal or ordinal scale should be used to diagnose RCT with US.</div></div><div><h3>Methods</h3><div>A systematic review was conducted in July 2024 according to the Preferred Reporting Items for Systematic reviews and Meta-Analyses guidelines. Studies were selected based on the following inclusion criteria: adults with RCT diagnosed with US, defined classification criteria for symptomatic tendinopathy, the US parameter used to measure structural change was reported with a grading scale (nominal or ordinal) and retrievable in full English text. Primary diagnosis of previous shoulder operations, partial or full-thickness tears, were excluded. Study quality was assessed using the Joanna Briggs Institute Critical Appraisal tools by two independent reviewers.</div></div><div><h3>Results</h3><div>A total of 1286 records were identified; eight studies met the inclusion criteria. The quality of the studies ranged from poor (12%) to good (88%). The most common US parameters were echogenicity (88%, <em>n</em> = 7), thickness (88%, <em>n</em> = 7), vascularity (50%, <em>n</em> = 4) and calcification (25%, <em>n</em> = 2). A nominal grading scale was most commonly utilised when assessing echogenicity (86%, <em>n</em> = 6 of 7), thickness (71%, <em>n</em> = 5 of 7) and calcification (100%, <em>n</em> = 2 of 2), while an ordinal scale was utilised in 100% of studies assessing vascularisation. Additionally, it was noted positioning and operator experience varied between studies.</div></div><div><h3>Conclusion</h3><div>This systematic review demonstrates the heterogeneity between methods used to diagnose RCT through US. Future studies should develop a standardised method to assess echogenicity, thickness, vascularisation and calcification in RCT, potentially using an ordinal scale to align with tendinopathy research in the lower limb.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 485-495"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145696395","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Reference Intervals of Lateral Ventricles for Extremely Pre-Term Infants: Multicenter Cohort Research","authors":"Junyi Yan ,&nbsp;Yang Gao ,&nbsp;Liangyu Guo ,&nbsp;Dongmei Liu ,&nbsp;Yuqi Tan ,&nbsp;Jingyi Lu ,&nbsp;Qiufang Tian ,&nbsp;Lingyu Yan ,&nbsp;Rong Gao ,&nbsp;Yulin Peng","doi":"10.1016/j.ultrasmedbio.2025.11.009","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.009","url":null,"abstract":"<div><h3>Objective</h3><div>To establish reference intervals and intervention cutoffs for lateral ventricles in extremely pre-mature infants.</div></div><div><h3>Methods</h3><div>This prospective study recruited neurologically healthy extremely pre-mature infants born or treated at two Chinese Grade 3A Hospitals between 2021 and 2024. Upon enrollment (U1), anterior horn width (AHW), ventricular index (VI) and thalamo-occipital distance (TOD) were measured to establish reference values and surgical intervention cutoffs. Four weeks later, the same indices were measured (U2). We analyzed the correlations between AHW, VI and TOD and independent variables in U1 data using multiple linear regressions and compared the time-varying trends of AHW, VI and TOD between U1 and U2 data via mixed linear models.</div></div><div><h3>Results</h3><div>A total of 353 extremely pre-mature infants were included. Special attention was paid to the 97th percentile and 4 mm above it, internationally regarded as upper reference limits and intervention cutoffs for post-hemorrhagic ventricular dilation, respectively. The AHW, VI and TOD were all positively correlated with birth weight (<em>p</em> values &lt;0.05) and increased over time (<em>p</em> values &lt;0.001).</div></div><div><h3>Conclusion</h3><div>The study presents reference values and intervention cutoffs for AHW, VI and TOD in extremely pre-mature infants and provides an extensive comparison of all available reference values.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 629-637"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145688443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Methodological Implementation of Ultrasound-Based Shear Wave Elastography in Individuals With Knee Osteoarthritis: A Scoping Review","authors":"Ryan Matthews ,&nbsp;Sophie Rayner ,&nbsp;Meaghan Hannigan ,&nbsp;Anne Doan ,&nbsp;Rebecca Moyer","doi":"10.1016/j.ultrasmedbio.2025.11.667","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.667","url":null,"abstract":"<div><div>Ultrasound shear wave elastography (SWE) enables the reliable assessment of tissue stiffness <em>in vivo</em>. Recently, SWE has emerged as a tool to study musculoskeletal tissue mechanics in healthy and pathological populations, such as individuals with knee osteoarthritis (KOA). No previous articles have explicitly reviewed the implementation of SWE methodology in KOA populations. Therefore, a scoping review of published literature was performed to investigate study equipment details, measurement protocols and participant/transducer positioning as they relate to SWE methodology. The databases PubMed, CINAHL, Scopus and Embase were searched for published literature, yielding <em>n</em> = 29 articles that met inclusion criteria. Results were summarized by tissue type under investigation (<em>n</em> = 7, muscle, tendon, adipose, multiple tissues, cartilage, nerve and joint capsule). Examined in over half of all included studies (55%), muscle was the predominant tissue under investigation, while relatively few studies focused on direct KOA-related structures, such as cartilage. Transducer type and orientation were the only relatively homogenous SWE methods, with 93% of all included studies using linear transducers and 91% of reporting studies using parallel orientation. Notable heterogeneity in SWE methods was present across device modes, settings, stiffness measures and body, knee and transducer positioning. These findings indicate that methodological variability remains a significant challenge for SWE research in KOA, potentially limiting the reproducibility and generalizability of the stiffness outcomes that drive our current understanding of tissue mechanics in this population. Collectively, these results highlight the need for tissue-specific SWE methodology standardization to enhance the reliability, comparability and clinical interpretability of SWE in KOA research.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 496-505"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145835103","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Abdominal Low-intensity Pulsed Ultrasound Therapy Mitigates Intestinal Damage and Microbial Dysbiosis in Diabetic Mice","authors":"Liang Tang ,&nbsp;Huiyuan Zhang ,&nbsp;Yanan Yu ,&nbsp;Mengshu Cao ,&nbsp;Xiushan Fan ,&nbsp;Jianzhong Guo ,&nbsp;Lijun Sun ,&nbsp;Dean Ta","doi":"10.1016/j.ultrasmedbio.2025.11.661","DOIUrl":"10.1016/j.ultrasmedbio.2025.11.661","url":null,"abstract":"<div><h3>Objective</h3><div>Diabetes impairs intestinal barrier integrity and disrupts gut microbiota balance, exacerbating hyperglycemia. While low-intensity pulsed ultrasound (LIPUS) has been shown to alleviate inflammatory bowel disease (IBD), its role in diabetes-related gut dysfunction remains unclear. This study aimed to investigate whether LIPUS could improve intestinal barrier function, modulate gut microbiota, and mitigate hyperglycemia in type 1 diabetic (T1D) mice.</div></div><div><h3>Methods</h3><div>Streptozotocin-induced type 1 diabetic (T1D) mice received a daily 20-min abdominal LIPUS treatment (30 mW/cm²) for 6 wk. Body weight and blood glucose levels were monitored weekly throughout the intervention period. After sacrifice, the intestinal morphology was examined, and barrier integrity was systematically evaluated. The composition of the gut microbiota was analyzed, along with the quantification of microbial-derived metabolites.</div></div><div><h3>Results</h3><div>T1D mice exhibited weight loss, hyperglycemia, intestinal damage, and microbial dysbiosis. LIPUS treatment upregulated the intestinal mRNA expression of ZO-1 and Occludin, enhancing barrier function. LIPUS increased the abundance of beneficial bacteria (<em>e.g.</em>, Lactobacillus, Intestinimonas) and the levels of short-chain fatty acids (acetate, propionate, butyrate), while reducing lipopolysaccharides. Crucially, LIPUS significantly lowered blood glucose levels in the diabetic mice.</div></div><div><h3>Conclusion</h3><div>These findings demonstrate that abdominal LIPUS restores gut barrier integrity and microbiota homeostasis, offering a novel therapeutic strategy for mitigating the progression of diabetes.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"52 3","pages":"Pages 673-683"},"PeriodicalIF":2.6,"publicationDate":"2026-03-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145866162","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}