Ultrasound in Medicine and Biology最新文献

{"title":"Editorial Advisory Board","authors":"","doi":"10.1016/S0301-5629(25)00098-5","DOIUrl":"10.1016/S0301-5629(25)00098-5","url":null,"abstract":"","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Page i"},"PeriodicalIF":2.4,"publicationDate":"2025-04-16","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143838711","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Low-Intensity Pulsed Ultrasound on Post-Traumatic Intra-Articular Knee Adhesions in Rats.","authors":"Ryo Nakahara, Akira Ito, Momoko Nagai-Tanima, Chia Tai, Zixi Zhao, Shixuan Xu, Fumika Miyamoto, Sachiko Abiko, Tomoki Aoyama, Hiroshi Kuroki","doi":"10.1016/j.ultrasmedbio.2025.03.008","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.03.008","url":null,"abstract":"<p><strong>Objective: </strong>Intra-articular adhesions (IAA) caused by trauma or surgical invasion commonly elicit pain and motor dysfunction. However, effective treatments for preventing IAA remain elusive. This study investigated the effects of low-intensity pulsed ultrasound (LIPUS) therapy on IAA after immobilization following trauma.</p><p><strong>Methods: </strong>A knee adhesion model was established in male Wistar rats, which were divided into LIPUS and sham groups. LIPUS was applied for 20 min/d (30 mW/cm² [spatial average temporal average], 1 MHz, duty cycle 20%, 5 times/wk, for 1, 2 and 3 wk). Another group of rats was treated with the same parameters for 3 or 7 d. After the treatment period, we evaluated the range of motion (ROM) of the knee joint, the length of the adhesion and the posterior knee joint capsule. RNA-seq and RT-quantitative polymerase chain reaction were performed to investigate the molecular mechanisms underlying the effects of LIPUS.</p><p><strong>Results: </strong>The knee ROM was significantly improved, and the adhesion length was reduced in the LIPUS group. RNA-seq identified 113 and 776 differentially expressed genes on days 3 and 7, respectively, highlighting pathways related to inflammatory, immune and fibrotic responses. IL-6 mRNA in the LIPUS group was significantly upregulated on day 3 and significantly downregulated at 1 wk. The TNFα, TGFβ and HIF1α levels did not differ between all groups. COL1A1 expression in the sham group significantly increased on day 7.</p><p><strong>Conclusion: </strong>These results indicate that LIPUS therapy may affect inflammatory and fibrotic pathways and may serve as a rehabilitation approach to prevent the development of IAA.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143812587","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Targeted, Condensed Lung Ultrasound Training Program for Image Interpretation: A Prospective Multicenter Observational Study in Intensive Care Unit Professionals.","authors":"Amne Mousa, Coen Plantinga, Siebe G Blok, Robin Mulder, Sylvia den Boer, Laura A E Cox, Tom Dormans, Marnix Kuindersma, Harm Scholten, Marcus J Schultz, Marry R Smit, Lieuwe D J Bos, Frederique Paulus, Pieter R Tuinman","doi":"10.1016/j.ultrasmedbio.2025.03.005","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.03.005","url":null,"abstract":"<p><strong>Introduction: </strong>Standardized training in lung ultrasound is lacking. Given time constraints and challenges in accessing training, there is a need for condensed, focused training programs. This study aims to assess the effect of a targeted, condensed two hour training session on the competency of intensive care unit (ICU) healthcare professionals in interpreting lung ultrasound clips.</p><p><strong>Methods: </strong>This is a multicenter prospective study in ICU healthcare professionals from six centers. Subjects received a two hour lung ultrasound training: a lecture on principles and recognition of (patho)physiological patterns followed by hands-on training. Subjects competency was tested using twenty pre-recorded lung ultrasound clips before and after training. Retainment of knowledge was tested after six to twelve months in a subset of subjects. Subjects were deemed competent if they reached a test score ≥80%.</p><p><strong>Results: </strong>Sixty-six subjects (49% intensivist, 44% with no lung ultrasound experience) were included. 61 subjects (92%) reached the predetermined competency level after training. After the training, the highest percentage of subjects (n = 27, 97%) who reached the competency threshold were those with no prior experience in lung ultrasound. Post-test scores were significantly higher than pre-test scores (87%, 95% CI (86, 89) vs 76%, 95% CI (73, 79), p < 0.001) with a median improvement of 9, 95% CI (7, 11) percentage points. After six to twelve months all retested subjects (n = 12) maintained competency.</p><p><strong>Conclusion: </strong>A short two hour training program may be sufficient for ICU healthcare professionals to achieve competency in lung ultrasound interpretation, even for subjects with no prior lung ultrasound experience. Further studies are needed to validate these findings in different settings and assess competency in bedside ultrasound acquisition.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-04-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143796800","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Ultrasound-Assisted Blood-Brain Barrier Opening Monitoring by Photoacoustic and Fluorescence Imaging Using Indocyanine Green.","authors":"Thomas Ador, Mylène Fournié, Sébastien Rigollet, Claire Counil, Vasile Stupar, Emmanuel L Barbier, Chantal Pichon, Anthony Delalande","doi":"10.1016/j.ultrasmedbio.2025.02.016","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.02.016","url":null,"abstract":"<p><strong>Objective: </strong>The blood-brain barrier (BBB) is a selectively permeable membrane that restricts drug delivery to the central nervous system. Focused ultrasound (FUS) combined with microbubbles (MBs) is a promising technique to transiently open the BBB, enabling therapeutic delivery. However, real-time monitoring of BBB permeability changes remains challenging. This study investigated the use of indocyanine green (ICG) as a bi-modal contrast agent for photoacoustic and fluorescence imaging to assess BBB opening and closure dynamics.</p><p><strong>Methods: </strong>BALB/c mice underwent FUS-mediated BBB opening with different doses of MBs and ICG administration. Photoacoustic and fluorescence imaging were performed at various time points post-FUS to evaluate ICG extravasation dynamics. Magnetic resonance imaging (MRI) with gadolinium contrast was used as the gold standard for BBB permeability assessment. The effect of MB dose and injection timing on BBB closure kinetics was analyzed.</p><p><strong>Results: </strong>Photoacoustic imaging provided reliable BBB monitoring within the first hour post-FUS, whereas fluorescence imaging was more effective at detecting ICG extravasation at 24 h. A strong correlation was observed between fluorescence intensity and MRI-based contrast enhancement, confirming BBB opening dynamics. BBB closure followed an exponential decay model, with a half-closure time of approximately 81 min. The degree of BBB opening was proportional to the MB dose administered.</p><p><strong>Conclusion: </strong>ICG-based photoacoustic and fluorescence imaging provide a non-invasive and cost-effective alternative to MRI for monitoring FUS-induced BBB opening. These techniques offer complementary temporal windows for assessment, improving the precision of BBB permeability evaluation in preclinical and potentially clinical applications.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-03-27","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143744252","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Hepatic Portal Venous Perfusion Imaging Using Vessel-Labeling Super-Resolution Ultrasound","authors":"Jinyu Yang ,&nbsp;Jiabin Zhang ,&nbsp;Jian An ,&nbsp;Feihong Dong ,&nbsp;Shuo Huang ,&nbsp;Wenyu Guo ,&nbsp;Wenli Zhang ,&nbsp;Yunlong Bao ,&nbsp;Jue Zhang","doi":"10.1016/j.ultrasmedbio.2025.01.019","DOIUrl":"10.1016/j.ultrasmedbio.2025.01.019","url":null,"abstract":"&lt;div&gt;&lt;h3&gt;Objective&lt;/h3&gt;&lt;div&gt;Blood flow imaging and perfusion assessment of the hepatic portal vein are critical for the diagnosis of several liver diseases, including cirrhosis, primary and metastatic liver tumors. However, perfusion imaging of the portal vein is challenging due to the unique dual blood supply system of the liver.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Methods&lt;/h3&gt;&lt;div&gt;We developed a novel method for specific perfusion imaging of the portal vein and downstream vessels, which was validated on healthy mice (n = 4). The right lobe of the liver in healthy mice was sequentially imaged using ultrafast plane-wave Doppler imaging and vascular labeling. In each experiment, mice were first injected with phase-change nanodroplets (PCNDs), followed immediately by ultrafast Doppler imaging to determine the imaging section and locate portal vein branches. Through an interactive process, portal vein branches were selected by mouse click for data acquisition of vessel-labeling ultrasound (VLUS) based on PCNDs. Subsequent arrival time calculations and super-resolution ultrasound (SRUS) imaging were performed offline. To demonstrate the specificity of the proposed method for vascular imaging, one mouse was injected with Sonovue microbubbles for plane-wave ultrasound data acquisition and microbubble-based VLUS data acquisition. All imaging experiments were conducted on the Verasonics (Kirkland, WA, USA) Vantage 256 ultrasound system using an L22-8v linear array transducer with a center frequency of 15.625 MHz. The multi-angle coherent compounding plane-wave acquisition frame rate was 500 Hz.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Results&lt;/h3&gt;&lt;div&gt;Imaging results from healthy mice (n = 4) demonstrated that VLUS was able to label different branches of the hepatic portal vein and specifically image downstream vessels. Analysis of the &lt;em&gt;in vivo&lt;/em&gt; results at different spatial scales showed that the brightness of the downstream perfusion area was significantly enhanced after labeling started, while there was no significant difference in image brightness before the labeling started and after it ended. By analyzing the acoustic field distribution at the focal point, the full width at half maximum in the x&lt;sub&gt;1&lt;/sub&gt; and z&lt;sub&gt;1&lt;/sub&gt; directions were 98.56 μm and 526.68 μm, respectively. Along the propagation path of the focused beam (outside the labeling area), no significant activation of the PCNDs was observed (&lt;em&gt;p&lt;/em&gt; &lt; 0.0001). Combined with SRUS technology, the resolution of the VLUS portal vein imaging results was further enhanced. The time-intensity curves of the downstream regions of interest indicated that VLUS provided a step input signal to the downstream vessels. Based on the arrival time of the step point in the time-intensity curves, the arrival time distribution map of the downstream vessels relative to the labeling point could be calculated.&lt;/div&gt;&lt;/div&gt;&lt;div&gt;&lt;h3&gt;Conclusion&lt;/h3&gt;&lt;div&gt;We propose a novel method for hepatic portal vein perfusion imaging based on VLUS. &lt;em","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Pages 951-960"},"PeriodicalIF":2.4,"publicationDate":"2025-03-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143721443","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Imaging Behind the Plaque: Improved Blood Flow Quantification Using an Iterative Scheme for Active Attenuation Correction","authors":"Jelle Plomp ,&nbsp;Ashkan Ghanbarzadeh-Dagheyan ,&nbsp;Michel Versluis ,&nbsp;Guillaume Lajoinie ,&nbsp;Erik Groot Jebbink","doi":"10.1016/j.ultrasmedbio.2025.02.012","DOIUrl":"10.1016/j.ultrasmedbio.2025.02.012","url":null,"abstract":"<div><h3>Objective</h3><div>Blood flow quantification using high frame-rate (HFR), contrast-enhanced ultrasound followed by particle image velocimetry (PIV), termed echoPIV, allows the study of blood flow phenomena in diseased arteries before and after treatment. However, acoustic shadows caused by atherosclerotic plaques may lead to incomplete flow quantification. As a global increase in transmit pressure to compensate for the attenuation would lead to contrast agent destruction in unattenuated areas, this article proposes a method to locally enhance the signal amplitude, thereby improving flow quantification accuracy.</div></div><div><h3>Methods</h3><div>The acoustic pressure was locally increased by adjusting the transmit apodization of the transducer elements using a proportional integral controller coupled to an acoustic model based on the Rayleigh integral. These iterative adjustments were performed prior to the HFR recording. This iterative scheme for active attenuation correction (ISAAC) was applied <em>in vitro</em> on phantoms with different levels of attenuation. A PIV analysis was then performed on each of the recorded HFR datasets.</div></div><div><h3>Results</h3><div>Without ISAAC, using a driving voltage of 11.2V, the mean errors in velocity estimates were below 20% for attenuation values up to 6.4 dB. Using ISAAC, the errors were reduced to less than 10% for attenuation values up to 8.5 dB and to less than 20% for attenuation up to 10.6 dB.</div></div><div><h3>Conclusion</h3><div>The proposed iterative scheme for attenuation correction was shown to compensate effectively for acoustic signal loss in acoustic shadows. ISAAC led to an improved accuracy in echoPIV-derived flow velocities.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Pages 984-998"},"PeriodicalIF":2.4,"publicationDate":"2025-03-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143671521","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Potential for Blood–Brain Barrier Disruption During Transcranial Ultrasound Super-Resolution Imaging","authors":"Hanjoo R. Lee ,&nbsp;Rebecca M. Jones ,&nbsp;Phillip G. Durham ,&nbsp;Virginie Papadopoulou ,&nbsp;Gianmarco F. Pinton ,&nbsp;Paul A. Dayton","doi":"10.1016/j.ultrasmedbio.2025.02.006","DOIUrl":"10.1016/j.ultrasmedbio.2025.02.006","url":null,"abstract":"<div><div>Super-resolution (SR) ultrasound imaging dramatically improves the resolution of microvascular images beyond the diffraction limit. It is well-established that ultrasound with contrast agents, such as those used in SR, can open the blood–brain barrier (BBB) under certain conditions. This study aimed to quantify potential BBB disruption (BBBD) during 3-D transcranial ultrasound SR as a function of mechanical index (MI) and pressure distribution. Mice were imaged using conventional 3-D SR sequences. BBBD was quantified through fluorescence imaging of dye extravasation for mechanical indices in the range of 0–0.78 (measured in water). The results suggest that when 3-D SR images are acquired at 0.78 MI, BBBD occurs; however, imaging was achievable without significant dye extravasation below 0.78. In addition, hydrophone measurements and 3-D simulations were performed to estimate local pressure distributions in the brain. For a transducer surface MI of 0.64, estimates of the local MI within the brain averaged 0.18 ± 0.09, ranging from 0.03 to 0.44, with no significant BBBD observed. However, for a transducer surface MI of 0.78, significant BBBD was observed (<em>p</em> &lt; 0.05), where the values in the brain range from 0.04 to 0.53, averaging 0.22 ± 0.11. This suggests that the local MI that generates BBB bio-effects is within the range of 0.44–0.53 MI. For the 0.78 MI case, 4.8% of the total brain volume had a pressure above 0.44 MI according to hydrophone measurements (4.8 mm² of the 100 mm² 2-D plane scanned) and 11.10% according to simulations (74.6 mm³ of the 777.5 mm³ volume).</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Pages 961-968"},"PeriodicalIF":2.4,"publicationDate":"2025-03-18","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143665195","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Two-dimensional Speckle Tracking Echocardiography and Fetal Cardiac Performance During Fetoscopic Repair of Myelomeningocele","authors":"Stephanie A. Eyerly-Webb ,&nbsp;Cathleen R. Pruitt ,&nbsp;Trisha Gipple ,&nbsp;Saul Snowise ,&nbsp;Emily F. Barthel ,&nbsp;Clifton O. Brock ,&nbsp;Joseph B. Lillegard ,&nbsp;James Fisher ,&nbsp;Benjamin Reynolds ,&nbsp;Kyle Halvorson ,&nbsp;Paige Reimche ,&nbsp;Eric Dion ,&nbsp;Lisa Howley","doi":"10.1016/j.ultrasmedbio.2025.02.009","DOIUrl":"10.1016/j.ultrasmedbio.2025.02.009","url":null,"abstract":"<div><h3>Objective</h3><div>No clinical standard exists for intraoperative fetal cardiac monitoring during maternal-fetal surgery for fetal myelomeningocele (fMMC). This pilot study explores the feasibility of using speckle tracking echocardiography (STE)-derived functional measurements to characterize cardiac performance throughout fetoscopic fMMC and compares these measures with other common intraoperative cardiac function parameters.</div></div><div><h3>Methods</h3><div>Continuous fetal echocardiography was performed during fetoscopic fMMC repair with fetal heart rate assessment every 2 minutes and a 4-chamber cine clip and mitral and tricuspid Doppler inflow patterns captured every 5 minutes. Offline postprocessing was used to measure functional data during fetal surgery including left ventricle (LV) global longitudinal strain (GLS), right ventricle (RV) GLS, LV ejection fraction (EF), and RV fractional area of change (FAC). Interrater agreement was determined for all measurements.</div></div><div><h3>Results</h3><div>Intraoperative fMMC echocardiograms were successfully obtained and analyzed for twenty patients. LV and RV GLS remained stable with a population average of -25.9±2.6% and -23.1±2.0% respectively during fMMC, and comparable to published normative ranges. Cardiac systolic and diastolic function was preserved at all surgical stages as demonstrated by LV EF, RV FAC, and spectral Doppler measurements. Interrater agreement for the spectral Doppler measurements was high, whereas STE agreement was low.</div></div><div><h3>Conclusion</h3><div>Fetal cardiac image acquisition for STE analysis was feasible during all stages of fetoscopic fMMC repair. Poor interrater agreement and offline post-processing limit the utility of STE for real-time clinical assessment, yet with experienced operators it could serve as a useful tool for scientific innovation to better understand modifiers of intraoperative fetal cardiac function.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Pages 977-983"},"PeriodicalIF":2.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143639837","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Editorial Advisory Board","authors":"","doi":"10.1016/S0301-5629(25)00074-2","DOIUrl":"10.1016/S0301-5629(25)00074-2","url":null,"abstract":"","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 5","pages":"Page i"},"PeriodicalIF":2.4,"publicationDate":"2025-03-15","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143628743","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Delivering Gd-Labeled IgG Antibodies Into the Mouse Brain Following Focused Ultrasound Treatment","authors":"Hasan Koruk ,&nbsp;Chris Payne ,&nbsp;Paul Cressey ,&nbsp;Maya Thanou ,&nbsp;Antonios N. Pouliopoulos","doi":"10.1016/j.ultrasmedbio.2025.02.015","DOIUrl":"10.1016/j.ultrasmedbio.2025.02.015","url":null,"abstract":"<div><h3>Objective</h3><div>Antibody-based therapy has emerged as a powerful tool for targeted treatment of neurological diseases, such as brain cancer and neurodegenerative disorders. However, direct, scalable, and safe confirmation of antibody delivery into the brain remains challenging. Antibodies can be effectively tracked when tagged with molecules that are detectable by medical imaging modalities, such as MRI, PET, or SPECT. In this study, we aimed to confirm gadolinium (Gd)-labeled IgG antibody delivery into the mouse brain using MRI, following exposure to focused ultrasound (FUS) and circulating microbubbles.</div></div><div><h3>Methods</h3><div>We acquired MR images of the mouse brain to evaluate antibody delivery into the targeted brain region. First, we quantified the MR signal of Gd-labeled IgG antibodies in phantoms using preclinical 9.4 T and clinical 3 T MRI scanners. Then, we determined optimal ultrasound and MR imaging parameters to non-invasively and safely disrupt the blood-brain barrier in a localized and reversible manner and effectively monitor antibody delivery into the murine brain, respectively.</div></div><div><h3>Results</h3><div>We confirmed that IgG antibodies can be reliably delivered into the murine brain using FUS and microbubble treatment and that we can track their biodistribution within the brain parenchyma using clinically relevant MR image sequences. The maximum detected volume of Gd-IgG antibody delivery (n = 4) was determined to be 0.12 ± 0.02 mm³ at t = 75.3 ± 17.3 minutes following treatment.</div></div><div><h3>Conclusion</h3><div>This work paves the way for a scalable and non-ionizing method for performing and evaluating antibody delivery into the brain.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 6","pages":"Pages 1018-1027"},"PeriodicalIF":2.4,"publicationDate":"2025-03-13","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143630902","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}