Ultrasound in Medicine and Biology最新文献

{"title":"Ultrasonic-Based Radiomics Signature With Machine Learning for Differentiating Prognostic Subsets of Pediatric Peripheral Neuroblastic Tumors: A Retrospective Study.","authors":"Hui Zhu, Jiazong Ye, Hongxia Luo, Shuangshuang Ni, Yin Pan, Zhilin Zhao, Yan Yang","doi":"10.1016/j.ultrasmedbio.2025.01.013","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.013","url":null,"abstract":"<p><strong>Objective: </strong>To construct and select a better model based on ultrasonic-based radiomics features and clinical characteristics for prognostic subsets of pediatric neuroblastic tumors.</p><p><strong>Methods: </strong>Data from 73 children with neuroblastic tumors were included and divided into a training group and a validation group. Data 1 contained the subjects' radiomics features and clinical characteristics, while data 2 contained radiomics features. With the help of machine learning, five models were constructed for data 1 and data 2, respectively. The model with the highest accuracy and area under the curve was selected as the combined model and radiomics model for data 1 and data 2, respectively. A superior model was then chosen from the models after further comparison.</p><p><strong>Results: </strong>The extreme gradient-boosting model for data 1 was chosen as the combined model and the extreme gradient-boosting model for data 2 was chosen as the radiomics model. The area under the curve of the combined and radiomics models in the validation group was 0.941 and 0.918 (p = 0.6906). The balanced accuracy, kappa value and F1 score of the radiomics model (0.9045, 0.8091 and 0.9091, respectively) were higher than those of the combined model (0.8545, 0.7123 and 0.8696, respectively). The top eight features of the radiomics model included five first-order statistical features and three textural features, all of which were high-dimensional features.</p><p><strong>Conclusion: </strong>Our study proved that the radiomics model outperformed the combined model at differentiating prognostic subsets of pediatric neuroblastic tumors. Additionally, we found that high-dimensional ultrasonic-based radiomics features surpassed other features and clinical characteristics.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383898","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Therapeutic Effects of Different Ultrasound Intensity Stimulation on Brown Adipose Tissue for the Treatment of Type 2 Diabetes.","authors":"Badamgarav Bat-Erdene, Min He, Jingsong Dong, Ying Li, Dean Ta","doi":"10.1016/j.ultrasmedbio.2025.01.010","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.010","url":null,"abstract":"<p><p>Type 2 diabetes (T2D) is a persistent illness that has a high incidence rate. Still, there is no conclusive evidence on effectively improving blood sugar levels in patients through physical therapy. This study examined the regulatory effects of different intensities of low-intensity pulsed ultrasound (LIPUS) on T2D by stimulating brown adipose tissue (BAT). Eight-week-old C57BL/6J mice were divided into six groups (n = 10 per group): Control sham (C-Sham), Control-LIPUS (C-LIPUS), T2D-sham (T2D-Sham), T2D groups treated with LIPUS at spatial average-temporal-average intensity (I_sata) of 60mW/cm² (T2D-L-60), 80mW/cm² (T2D-L-80), and 100mW/cm² (T2D-L-100). T2D models were induced by intraperitoneal injection of 40 mg/kg streptozotocin (STZ) three times after 12 wks of high-fat diet (HFD). The T2D-LIPUS group received LIPUS stimulation for 20 minutes per day for 6 weeks. The LIPUS stimulation had a duty cycle of 20%, a frequency of 1 MHz, and I_sata of 60mW/cm², 80mW/cm², 100mW/cm². Subsequently, glucose tolerance tests (GTT) and insulin tolerance tests (ITT) were performed, and body fat content in mice was analyzed using nuclear magnetic resonance (NMR). Metabolic changes were monitored using metabolic cages. The results indicated that 80mW/cm² intensity level significantly improved glucose tolerance, insulin sensitivity, and metabolic function after LIPUS exposure. Significant reductions in body fat content and enhanced thermogenesis were observed, highlighting the potential of LIPUS in T2D management. This provides the basis for the dose study of LIPUS in the treatment of T2D.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-08","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143383897","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Safety of Shear Wave Elastography as Evidenced From Carotid Artery Strain and Strain Rate Induced by Acoustic Radiation Force Impulse and Arterial Pulsations.","authors":"Tim Nordenfur, Kenneth Caidahl, Linnea Lindberg, Matthew W Urban, Matilda Larsson","doi":"10.1016/j.ultrasmedbio.2024.12.004","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2024.12.004","url":null,"abstract":"<p><strong>Objective: </strong>The aim of this study was to investigate the mechanical effects of carotid shear wave elastography (SWE) in vivo as its effects on the arterial wall have not been thoroughly examined.</p><p><strong>Methods: </strong>We evaluated the mechanical effects of carotid SWE in vivo in terms of the radial strain and strain rate to which acoustic radiation force impulses (ARFIs) expose the arterial wall, and compared them with the strain and strain rate induced by arterial pulsation in 13 healthy study subjects (seven individuals 20-35 y of age and six individuals 50-65 y of age). Additionally, we explored whether mechanical effects vary with timing of ARFI and subject age.</p><p><strong>Results: </strong>The young cohort was found to have, compared with the old cohort, a higher diastolic ARFI-induced peak strain (p = 0.002) and peak strain rate (p = 0.001), and a lower diastolic ARFI-induced peak negative strain rate (p = 0.013). When comparing cardiac phases, diastolic ARFIs were found to induce a lower peak negative strain rate than systolic ARFIs (p = 0.006). Importantly, ARFI-induced peak strain was lower than that caused by arterial pulsation in both age cohorts (p < 0.0001). The ARFI-induced peak strain rate was slightly higher than that caused by arterial pulsation at rest but lower than published exercise data. The ARFI-induced peak negative strain rate was similar to that caused by arterial pulsation.</p><p><strong>Conclusion: </strong>Our results indicate that arterial SWE does not expose the arterial wall to any higher strain or strain rate than is experienced during normal arterial pulsation. Further research is required to validate the results in arteries containing vulnerable plaques.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-06","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143371257","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Diagnosis of Salivary Gland Tumors Using Ultrasound Radiomics.","authors":"Ping-Chia Cheng, Wu-Chia Lo, Li-Jen Liao, Huihua Kenny Chiang","doi":"10.1016/j.ultrasmedbio.2025.01.008","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.008","url":null,"abstract":"<p><strong>Objective: </strong>The current diagnosis of salivary gland tumors (SGTs) is dependent on subjective ultrasound features. Here we aimed to develop an objective method using ultrasound radiomics.</p><p><strong>Methods: </strong>We collected 248 benign and 46 malignant images and divided them into training (80%) and testing (20%) groups, with 105 radiomic features extracted from each image. Data re-sampling, feature selection and classification were conducted. The diagnostic accuracy of different combinations was evaluated.</p><p><strong>Results: </strong>After data re-sampling using the Synthetic Minority Over Sampling Technique (SMOTE) and feature selection with LASSO+ANOVA, 10 radiomic features were selected. Using the Random Forest classifier, the testing set achieved an area under the receiver operating characteristic curve of 0.85, accuracy of 90%, sensitivity of 78% and specificity of 92% for diagnosing SGTs. It maintained an accuracy of 85% in a separate internal validation set.</p><p><strong>Conclusion: </strong>This study offers significant insights into the use of radiomics for the diagnosis of SGTs. When selected properly and paired with a suitable classification model, radiomics can be used to differentiate between benign and malignant SGTs.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256960","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-intensity Pulsed Ultrasound Stimulation of the Intestine Improves Insulin Resistance in Type 2 Diabetes.","authors":"Huan Wang, Min He, Badamgarav Bat-Erdene, Ying Li, Dean Ta","doi":"10.1016/j.ultrasmedbio.2025.01.004","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.004","url":null,"abstract":"<p><strong>Objective: </strong>Ultrasound stimulation of internal organs and peripheral nerves has demonstrated promising potential in regulating blood glucose metabolism. This study aims to assess the effectiveness of low-intensity pulsed ultrasound stimulation (LIPUS) on intestine in improving insulin resistance with type 2 diabetes mellitus (T2DM).</p><p><strong>Methods: </strong>C57BL/6J mice, both normal and T2DM, were randomly divided into three groups: Control, T2D-sham, and T2D-LIPUS. The T2D-LIPUS group received LIPUS stimulation in the intestine. The parameters were as follows: 1 MHz frequency, 1.0 kHz pulse repetition frequency (PRF), 20% duty cycle, 100 mW/cm² intensity spatial average temporal average (I_SATA), for 20 minutes per session, five days per week, over four weeks.</p><p><strong>Results: </strong>Blood glucose analysis indicated that mice in the T2D-LIPUS group displayed significantly lower area under the curve (AUC) of glucose tolerance tests (GTT) and insulin tolerance tests (ITT) (p < 0.001), HOMA-IR (p < 0.001), and fasting serum insulin levels (p < 0.01) compared to the T2D-sham group. LIPUS treatment effectively lowered serum levels of IL-1β (p < 0.001) and TNF-α (p < 0.01) along with mRNA expression levels of IL-1β (p < 0.01) and IL-18 (p < 0.001) in the intestines of T2DM mice. Additionally, Western blot analysis revealed a reduction in the protein levels of NLRP3, caspase-1, and GSDMD-N in the intestinal tissues of mice treated with LIPUS.</p><p><strong>Conclusion: </strong>These findings suggest that LIPUS can reduce inflammation and cellular apoptosis, while improving insulin resistance by inhibiting the NLRP3/Caspase-1/GSDMD signaling pathway. This research introduces a novel, non-pharmacological approach for managing T2DM.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-05","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143366343","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sonosensitizers for Sonodynamic Therapy: Current Progress and Future Perspectives.","authors":"Ting Wang, Meng Du, Zhiyi Chen","doi":"10.1016/j.ultrasmedbio.2025.01.011","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.011","url":null,"abstract":"<p><p>Sonodynamic therapy (SDT) is a novel non-invasive treatment method that combines low-intensity ultrasound and sonosensitizers. Compared with photodynamic therapy, SDT has the advantages of deeper tissue penetration, higher accuracy and fewer adverse reactions. Sonosensitizers are essential for the efficacy of SDT. Sonosensitizers have the advantages of clear structure, easy monitoring, evaluation of drug metabolism and clinical transformation, etc. Notably, biochemical techniques can be used in the field of sonosensitizers and SDT to overcome inherent barriers and achieve sustainable innovation. This article first summarizes the molecular mechanism of SDT, focusing on organic sonosensitizers, inorganic nano-sonosensitizers and multi-functional drug delivery systems with targeting, penetration and imaging functions after a series of modifications. This review provides ideas and references for the design of sonosensitizers and SDT and promotes their future transformation into clinical applications.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-04","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143256961","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Application of B+M-Mode Ultrasound in Evaluating Dysphagia in Elderly Stroke Patients","authors":"Jiaying Shuai ,&nbsp;Linping Pian ,&nbsp;Li Tian ,&nbsp;Linying Wang ,&nbsp;Miaomiao Deng ,&nbsp;Chen Cheng","doi":"10.1016/j.ultrasmedbio.2024.10.001","DOIUrl":"10.1016/j.ultrasmedbio.2024.10.001","url":null,"abstract":"<div><h3>Objective</h3><div>The purpose of this study was to evaluate and compare the swallow-related muscles and bones movement patterns during swallowing quantitatively by B+M-mode ultrasound, and to investigate its application value in dysphagia.</div></div><div><h3>Methods</h3><div>Sixty elderly stroke patients with dysphagia (patient group) and sixty healthy elderly individuals (control group) were enrolled in this study. M-mode ultrasound was utilized to measure the radial displacement and duration of tongue and geniohyoid muscle movements. B-mode ultrasound was employed to assess hyoid bone displacement and the distance of hyoid-larynx approximation. Ultrasound parameters were compared between the two groups. Logistic regression was used to identify the optimal parameters with independent predictive value. The predictive efficacy of each parameter for dysphagia was evaluated using ROC curve analysis.</div></div><div><h3>Results</h3><div>Both intraindividual and interindividual variations were observed in the B+M-mode imaging traces during the three swallows. Significant differences were found in the average radial displacement and duration of tongue and geniohyoid muscle movements, hyoid bone displacement, and hyoid-larynx approximation distance between the two groups (all <em>p</em> &lt; 0.001). The radial displacement of the tongue and geniohyoid muscle, hyoid bone displacement, and hyoid-larynx approximation distance were lower in the patient group compared to the control group, while the duration of tongue and geniohyoid muscle movements was higher in the patient group. Logistic regression selected the radial displacement of the tongue, the duration of geniohyoid muscle movement, and the hyoid-larynx approximation distance as significant predictors. The combined model demonstrated excellent predictive performance with an AUC of 0.994 and good model fit (<em>p</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>The B+M-mode ultrasound offers a rapid and safe technique for the preliminary evaluation of swallowing movements. It can serve as a quantitative and noninvasive method for the clinical screening of dysphagia.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 2","pages":"Pages 273-279"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142607159","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Dyssynchronous Fetal Heart Failure in Maternal Diabetes: Evaluation with Speckle Tracking Echocardiography and Novel M-Mode Software","authors":"Theresa M. Kühle ,&nbsp;Angela Burgmair ,&nbsp;Georg Schummers ,&nbsp;Mareike Möllers ,&nbsp;Kathrin Oelmeier ,&nbsp;Chiara De Santis ,&nbsp;Helen Ann Köster ,&nbsp;Ute Möllmann ,&nbsp;Daniela Willy ,&nbsp;Janina Braun ,&nbsp;Felix Albert ,&nbsp;Ralf Schmitz","doi":"10.1016/j.ultrasmedbio.2024.10.004","DOIUrl":"10.1016/j.ultrasmedbio.2024.10.004","url":null,"abstract":"<div><h3>Objectives</h3><div>This study aimed to investigate dyssynchronous heart failure in fetuses of mothers with diabetes mellitus (FDM) and fetal controls (FC) using two-dimensional speckle tracking echocardiography (2D-STE) and novel M-mode prototype software (PS).</div></div><div><h3>Methods</h3><div>In this cohort study 174 fetuses were analyzed, 87 in the FDM-cohort and 87 gestational age-matched fetuses in the FC-cohort. A subgroup of 38 fetuses formed the final case group, with a high median frame rate of approximately 160 frames/s. Using 2D Cardiac Performance Analysis software (TOMTEC, Unterschleissheim, Germany) we measured global longitudinal strain (GLS). TOMTEC PS detected annular displacement by assessing an artificial M-mode on the previously generated tracking. Dyssynchrony (DYS) was calculated as the inter- and intraventricular difference in time to peak GLS or annular displacement.</div></div><div><h3>Results</h3><div>Greater DYS was observed in all basal myocardial measurement sites and software between FDM-cohort compared to FC-cohort and no significant correlation was found between DYS measurements and gestational age. Intraventricular DYS between the basal segments was statistically significant (all <em>p</em> ≤ 0.036, Wald test of univariate regression models). The PS performed best in DYS measurements identifying right ventricular DYS as potentially predicting FDM (FDM: median, 18.5 (interquartile range [IQR], 13.9–25.0) ms vs. FC: median, 2.7 [IQR, 1.5–3.5] ms; <em>p</em> &lt; 0.001).</div></div><div><h3>Conclusion</h3><div>Increased intraventricular DYS demonstrated an impact of maternal diabetes mellitus on fetal hearts independent of gestational age. The prototype M-mode method identified cardiac dysfunction with higher accuracy than the conventional analysis. High-quality echocardiographic image acquisition is imperative for clinical application of 2D-STE and related advanced technologies.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 2","pages":"Pages 302-311"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631398","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"OA","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"The Relationship Between Rim-like Enhancement on Pre-ablation Contrast-enhanced Ultrasound of Colorectal Liver Metastasis and Early Intrahepatic Progression After Thermal Ablation: A Preliminary Study","authors":"Jia Lin ,&nbsp;Huahui Liu ,&nbsp;Shuang Liang ,&nbsp;Liping Luo ,&nbsp;Sainan Guan ,&nbsp;Shanshan Wu ,&nbsp;Ying Liu ,&nbsp;Shuxian Xu ,&nbsp;Ronghua Yan ,&nbsp;Erjiao Xu","doi":"10.1016/j.ultrasmedbio.2024.10.015","DOIUrl":"10.1016/j.ultrasmedbio.2024.10.015","url":null,"abstract":"<div><h3>Objective</h3><div>To investigate the relationship between the rim-like enhancement pattern on pre-ablation contrast-enhanced ultrasound (CEUS) of colorectal liver metastasis (CRLM) and the therapeutic efficacy of percutaneous microwave ablation (MWA).</div></div><div><h3>Methods</h3><div>Patients with CRLM underwent MWA and were evaluated using CEUS before ablation in our hospital between February 2020 and February 2023 were enrolled in this retrospective study. The enhancement patterns of CRLM were assessed by two radiologists and classified as rim-like enhancement and non-rim-like enhancement patterns. The therapeutic outcomes, including cumulative intrahepatic progression rate and early intrahepatic progression rate, were followed up and analyzed.</div></div><div><h3>Results</h3><div>Overall, 50 patients with 121 nodules were enrolled. Rim-like enhancement pattern was observed in 18 patients (18/50, 36.0%). The cumulative intrahepatic progression rate was significantly higher in the rim-like enhancement group than the rate in the non-rim-like enhancement group (<em>p</em> = 0.022). The early intrahepatic progression rate in the rim-like enhancement group was also significantly higher than the rate in the non-rim-like enhancement group (12/17, 70.6% vs. 6/24, 25.0%, <em>p</em> = 0.005). The multivariable analysis demonstrated that the rim-like enhancement pattern of CRLM was a significant risk factor associated with early intrahepatic progression after MWA (<em>p</em> = 0.013).</div></div><div><h3>Conclusion</h3><div>Rim-like enhancement pattern on pre-ablation CEUS of CRLM was associated with a higher risk of intrahepatic progression after MWA.</div></div>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":"51 2","pages":"Pages 358-363"},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"142631401","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Low-Intensity Ultrasound Facilitation of Intranasal Drug Delivery to Olfactory Bulb and Trigeminal Nerves.","authors":"Meng-Ting Lin, Tsai-Yun Chan, Wei-Hao Liao, Chueh-Hung Wu, Tai-Horng Young, Wen-Shiang Chen","doi":"10.1016/j.ultrasmedbio.2025.01.003","DOIUrl":"https://doi.org/10.1016/j.ultrasmedbio.2025.01.003","url":null,"abstract":"<p><strong>Objective: </strong>Nasal-to-brain (NtoB) delivery is a noninvasive approach that uses the nasal cavity as a pathway to transport therapeutic agents directly to the brain. This approach bypasses systemic circulation and avoids the blood-brain barrier (BBB). Transcranial ultrasound, coupled with microbubbles (MB), is a technique used to oscillate and generate acoustic cavitation to open the capillary tight junctions of BBB temporarily. Its efficacy in facilitating NtoB delivery has been demonstrated in vivo. However, while opening the BBB, sonication with MB poses the risk of cerebral microhemorrhage or brain tissue damage due to sonication-induced physical injury. This study aimed to assess the effectiveness of low-intensity ultrasound treatment to facilitate NtoB delivery in a mouse model without using MB.</p><p><strong>Methods: </strong>In this study, 10-kDa dextran was administered intranasally (IN), and transcranial planar US was applied to the entire mouse brain without MB assistance. Ex-vivo whole brain imaging via fluorescence macroscopy, brain slice analysis with fluorescence microscope, and quantification of dextran concentration in distinct brain regions were conducted to compare the IN-only, IN combined with US (IN+US), and sham groups. For the trigeminal nerves (TN), fluorescence macroscopy, microscopy, and TN concentration quantification were performed to compare the three groups.</p><p><strong>Results: </strong>Whole brain imaging revealed that US facilitated the IN delivery of dextran to the olfactory bulb (OB) in the IN+US group compared with that in the IN-only and sham groups; however, this difference was not observed after a 24 h follow-up. Conversely, brain slice images showed that the tracer was delivered to the OB, cerebral cortex, striatum and brainstem in the IN+US group, but this finding was not observed in the IN-only group at the 4 h mark. The quantification of fluorescence intensity at two follow-up time points revealed no significant difference between the IN and IN+US groups in these specific regions. Dextran concentration analysis for distinct brain areas and TN showed that ultrasound significantly increased the tracer concentration delivered to the OB and TN in the IN+US group at the 4 h mark compared with that in the IN-only and sham groups; however, this effect was not sustained at 24 h. Confocal microscopy indicated that the dextran tracer accumulated in the perivascular space along the microvascular structures.</p><p><strong>Conclusion: </strong>We demonstrated the efficacy of low-intensity ultrasound without using MB, in enhancing nose-to-OB and nose-to-TN drug delivery, and proposed the potential for future clinical application. Thus, we showed that this approach was safe, without evidence of microhemorrhage or brain tissue damage.</p>","PeriodicalId":49399,"journal":{"name":"Ultrasound in Medicine and Biology","volume":" ","pages":""},"PeriodicalIF":2.4,"publicationDate":"2025-02-01","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"143081819","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"医学","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}