Noninvasive Ultrasound Study of Acute Myocardial Infarction in Different Pathological Phases.

IF 2.4 3区医学 Q2 ACOUSTICS

Ultrasound in Medicine and Biology Pub Date : 2025-06-14 DOI:10.1016/j.ultrasmedbio.2025.05.021

Zihang Wang, Ting Ma, Yuanyuan Yang, Guodong Wang, Lina Guan, Baihetiya Tayier, Yuming Mu

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引用次数: 0

Abstract

Objective: By evaluating the myocardial mechanics and microcirculation perfusion in different pathological phases of acute myocardial infarction (AMI) using speckle tracking imaging (STI) and myocardial contrast echocardiography (MCE). we aim to provide a non-invasive ultrasound diagnosis strategy to distinguish different pathological phases of AMI.

Methods: The C57/BL6 mouse model of acute myocardial infarction (AMI) was created by ligating the left anterior descending coronary artery. Microvessel density (MVD) and pathological changes in the infarct area were analyzed quantitatively and qualitatively using TTC, HE, Masson staining, immunohistochemistry, and immunofluorescence during the inflammatory, proliferative, and mature phases. Global longitudinal strain (GLS), circumferential strain (GCS), and radial strain (GRS) were measured by STI, while myocardial perfusion peak enhancement (PE) and Wish-in-rate (WIR) were measured by MCE.

Results: For the inflammatory phase, MVD was 52.8/0.1mm², and inflammatory factors IL-6, IL-1β, and TNFα were up-regulated. WIR showed the best accuracy at this phase, with a value of 12.29 ± 2.24 dB, AUC of 0.93, sensitivity of 1.00, specificity of 0.83, and cut-off of 9.24. For the proliferative phase, MVD dropped to 21.6/0.1mm²; GLS and WIR had high diagnostic performance, with AUCs of 0.89 and 0.97, sensitivities of 0.75 and 1.00, and specificities of 1.00 and 0.91, respectively. In the mature phase, the infarct area became fibrotic, inflammatory factors disappeared, and MVD decreased to 8.6/0.1mm². GRS analysis has diagnostic value, with an AUC of 0.77, sensitivity of 0.91, specificity of 0.66, and a cut-off of 14.36. PE maintained high diagnostic accuracy, with an AUC of 0.93, sensitivity of 1.00, specificity of 0.75, and a cut-off of 5.47.

Conclusion: The combination of MCE and STI can act as a reliable non-invasive ultrasound diagnosis to distinguish different pathological phases of AMI.

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急性心肌梗死不同病理阶段的无创超声研究。

目的：应用散斑跟踪成像（STI）和心肌超声造影（MCE）评价急性心肌梗死（AMI）不同病理阶段心肌力学和微循环灌注的变化。我们的目的是提供一种无创超声诊断策略来区分AMI的不同病理阶段。方法：结扎左冠状动脉前降支，建立C57/BL6小鼠急性心肌梗死模型。采用TTC、HE、Masson染色、免疫组织化学、免疫荧光定量定性分析梗死区炎症期、增殖期和成熟期微血管密度（MVD）和病理变化。STI法测量心肌整体纵向应变（GLS）、周向应变（GCS）和径向应变（GRS）， MCE法测量心肌灌注峰值增强（PE）和wish -in率（WIR）。结果：炎症期MVD为52.8/0.1mm²，炎性因子IL-6、IL-1β、TNFα上调。WIR在这一阶段的准确度最高，其值为12.29±2.24 dB， AUC为0.93，灵敏度为1.00，特异性为0.83，截止值为9.24。增生期MVD降至21.6/0.1mm²；GLS和WIR具有较高的诊断效能，auc分别为0.89和0.97，灵敏度分别为0.75和1.00，特异性分别为1.00和0.91。成熟期梗死区纤维化，炎性因子消失，MVD降至8.6/0.1mm²。GRS分析具有诊断价值，AUC为0.77，敏感性为0.91，特异性为0.66，cut-off为14.36。PE保持了较高的诊断准确性，AUC为0.93，敏感性为1.00，特异性为0.75，cut-off为5.47。结论：MCE联合STI可作为一种可靠的无创超声诊断方法来区分AMI的不同病理分期。

本文章由计算机程序翻译，如有差异，请以英文原文为准。

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来源期刊

Ultrasound in Medicine and Biology 医学-核医学

CiteScore

6.20

自引率

6.90%

发文量

325

审稿时长

70 days

期刊介绍： Ultrasound in Medicine and Biology is the official journal of the World Federation for Ultrasound in Medicine and Biology. The journal publishes original contributions that demonstrate a novel application of an existing ultrasound technology in clinical diagnostic, interventional and therapeutic applications, new and improved clinical techniques, the physics, engineering and technology of ultrasound in medicine and biology, and the interactions between ultrasound and biological systems, including bioeffects. Papers that simply utilize standard diagnostic ultrasound as a measuring tool will be considered out of scope. Extended critical reviews of subjects of contemporary interest in the field are also published, in addition to occasional editorial articles, clinical and technical notes, book reviews, letters to the editor and a calendar of forthcoming meetings. It is the aim of the journal fully to meet the information and publication requirements of the clinicians, scientists, engineers and other professionals who constitute the biomedical ultrasonic community.