BioEnergy Research最新文献

{"title":"Electrospun Cellulase Nanofibers for Continuous Hydrolysis of Bermuda Grass for Biofuels","authors":"Vedavarshini Narayanan,&nbsp;Harini Saravanan,&nbsp;Sri Sundar Rajan K,&nbsp;Sri Sakthi Ashwin R M,&nbsp;Piyush Singh,&nbsp;Aarthi P. A.,&nbsp;Kiran Babu Uppuluri","doi":"10.1007/s12155-025-10877-1","DOIUrl":"10.1007/s12155-025-10877-1","url":null,"abstract":"<div><p>The increasing demand for stable biocatalysts in lignocellulose hydrolysis highlights the potential of onsite-produced fungal enzyme cocktails. Immobilization of these enzymes can improve their stability, enable reusability, and reduce costs, making them a viable option for sustainable industrial processes. The present study explored the direct encapsulation of the partially purified cellulase obtained from <i>Trichoderma harzianum</i> BPGF1 with polyvinyl alcohol (PVA) into nanofibers through electrospinning. The optimized solution composition for membrane synthesis was determined to be 13% (w/v) PVA and 25% (v/v) of the enzyme. The scanning electron microscopy confirmed the encapsulated enzymes in the nanofiber. The wettability of the electrospun enzyme nanofiber (ENF) was found to be 37.78°, which indicates the hydrophilicity. Young’s modulus of ENF was found to be 28.39 MPa, indicating reliable tensile strength. The effects of pH and temperature were studied on the ENF, and the maximum enzyme activity was observed at pH 7.0 and 70 °C, respectively, when compared with the free enzyme. The kinetic parameters were determined using Michaelis–Menten kinetics. The ENF was tested in the hydrolysis of pretreated <i>Cynodon dactylon</i> grass into fermentable sugars to produce biofuels. The ENF retained 50% of its initial enzyme activity even after 20 cycles, promising its application in continuous hydrolysis.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145162559","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Synthesis of Hydroxycinnamic Acid Esters Integrated with Aqueous Two-Phase System and Production of Fermentable Sugars from Corncobs","authors":"Lucas Felipe Simões Silva,&nbsp;Jadna Lúcia de Freitas Silva,&nbsp;Matheus Galvão Corcino,&nbsp;Filipe de Melo Solon,&nbsp;Gleyson Batista de Oliveira,&nbsp;Emmanuel Damilano Dutra,&nbsp;Elisama Vieira dos Santos,&nbsp;Domingos Fabiano de Santana Souza,&nbsp;Carlos Eduardo de Araújo Padilha","doi":"10.1007/s12155-025-10881-5","DOIUrl":"10.1007/s12155-025-10881-5","url":null,"abstract":"<div><p>The fractionation of lignocellulosic biomasses has been widely reported as a way to establish biorefineries. However, more efforts are needed to valorize lignin and its phenolic derivatives in the production chain. Thus, the present study investigated the production of hydroxycinnamic acid esters and monosaccharides from an integrated scheme using corncob as raw material. After alkaline pretreatment, p-coumaric acid and ferulic acid were released in the liquid fraction (1.91 and 0.81 mg/mL), and they were isolated/concentrated in an aqueous two-phase system (ATPS) ethanol/ammonium sulfate. The best ATPS led to a high recovery in the top phase, corresponding to 89.7% for p-coumaric acid and 90.5% for ferulic acid. Direct esterification of the ATPS top phase with 20% (v/v) sulfuric acid guaranteed 1.60 mg/mL ethyl p-coumarate and 0.67 mg/mL ethyl ferulate, both products with potential application in cosmetics. Xylan was recovered in the ATPS and was successfully converted into xylose via acid hydrolysis (yield of 69% under 10% solids). The pretreated corncob was digested to obtain 99.6 g/L glucose under 30% solids in fed-batch mode using cellulase loading of 5 FPU/g and 2% (w/w) Tween 80. These results are in line with the idea of ​​comprehensive use of lignocellulosic biomass, especially in the development of lignin-derived products with high added value.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145162560","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Optimization of Fuel Injection Parameters for Enhanced Performance and Emission Reduction in a Diesel Engine Using Animal Fat–Based Biodiesel Blends","authors":"Ratchagaraja Dhairiyasamy,&nbsp;Saurav Dixit,&nbsp;Deekshant Varshney,&nbsp;Deepika Gabiriel","doi":"10.1007/s12155-025-10878-0","DOIUrl":"10.1007/s12155-025-10878-0","url":null,"abstract":"<div><p>This study investigates the performance and emission characteristics of a diesel engine using a blend of 20% animal fat-based biodiesel (AFB20) under various FIPs (fuel injection pressures) and FITs (fuel injection timing). The objective was to optimize these parameters to enhance engine efficiency and reduce emissions. The experimental setup included a vertical, single-cylinder, four-stroke diesel engine equipped with an eddy current dynamometer and an exhaust gas analyzer. Methods involved varying the FIP between 180 and 240 bar and the FIT from 19 to 27° before the top dead center (TDC). Key performance indicators such as BSFC, BTE, and EGT were measured. Emissions of CO, UBHC, NOx, and smoke were also recorded. Results showed that at 200 bar FIP, the engine exhibited the lowest BSFC of 0.26 g/kWh and the highest BTE of 32.57%. The optimal FIT was 23° bTDC, achieving a BTE of 33.8% and a significant reduction in NOx emissions by 15.07%. Higher FIPs improved atomization, leading to better combustion and lower emissions of UBHC and CO. However, NOx emissions were higher at increased FIPs due to higher combustion temperatures. The study concludes that a FIP of 200 bar and a FIT of 23° bTDC are optimal for achieving improved engine performance and reduced emissions.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145163062","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Theoretical Analysis of the Hydrolysis Mechanism of Biopolymers in the Anaerobic Digestion Process of Livestock Manure","authors":"Sebastián Villegas-Moncada,&nbsp;Mario Luna-delRisco,&nbsp;Catalina Arroyave-Quiceno,&nbsp;Mauricio González-Palacio,&nbsp;Carlos Peláez-Jaramillo","doi":"10.1007/s12155-025-10873-5","DOIUrl":"10.1007/s12155-025-10873-5","url":null,"abstract":"<div><p>Over the past two decades, modeling the hydrolysis stage has been recognized as critical for understanding its behavior and determining optimal operating conditions for anaerobic digestion (AD). Traditional approaches, such as first-order and Michaelis–Menten kinetic models, account for substrate concentration and enzymatic activity, respectively, but neglect mass-transfer effects. In this work, we propose a semi-empirical model that integrates enzymatic catalysis with molecular diffusion phenomena in the microbial boundary layer. We derive a hydrolysis rate expression by combining Michaelis–Menten kinetics with Fick’s law of diffusion and validate it against experimental data from a thermophilic batch reactor treating cattle manure (55 <span>(^{circ })</span>C, 62 <span>(g,text {VS},text {L}^{-1})</span>). Compared to the first-order model (<i>R</i><span>(^2)</span> = 0.940), our model achieves a superior fit (<i>R</i><span>(^2)</span> = 0.973), demonstrating that diffusion resistance can significantly influence hydrolysis kinetics. By formulating the kinetic model in terms of explicit biochemical and mass-transfer parameters (<span>(r_{h,text {max}})</span>, <span>(K_M)</span>, <span>(k_d)</span>, <span>(alpha )</span>), it becomes possible to identify optimal operational strategies for enhancing hydrolysis efficiency. The results indicate that coupling enzymatic catalysis with diffusion provides a more accurate theoretical description than the first-order model and enables improved prediction of biopolymer solubilization in AD.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145163064","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Functional Properties Optimization of Carboxymethylcellulose-Based Films Enriched with Bioactive Compounds from Olive Leaf Co-products","authors":"Hajer Aloui,&nbsp;Yassine M’Rabet,&nbsp;Monia El Ouni,&nbsp;Khaoula Khwaldia","doi":"10.1007/s12155-025-10871-7","DOIUrl":"10.1007/s12155-025-10871-7","url":null,"abstract":"<div><p>Composite carboxymethyl cellulose (CMC)-based films containing olive leaf extract (OLE) and the polysaccharide fraction from olive leaves (PFOL) were produced by a solvent casting method. The effects of OLE and PFOL contents on physical and functional properties of CMC composite films were assessed using a two-factor, five-level central composite design. Overall, water barrier properties of CMC-based films were significantly improved upon OLE and PFOL incorporation as confirmed by the observed reduction in their water absorption and water vapor permeability (WVP). Likewise, increasing PFOL content from 0 to 60 wt% significantly enhanced the flexibility of CMC/PFOL composite films, most likely due the increased mobility of CMC chains due to the formation of new hydrogen bonds between the CMC chains and the phenolic compounds in PFOL. Furthermore, the incorporation of OLE and PFOL endowed the CMC composite films with higher antioxidant capacity and excellent light barrier properties. Using the desirability function approach, an OLE contents of 0.51 wt% and 59.85 wt% PFOL were identified as the optimum factor levels providing maximum %<i>E</i> (23.28%), higher antioxidant activity (1.1 µmol TE/mg extract), and minimum WVP (17.62 g mm m⁻² j⁻¹ kPa⁻¹), with an overall desirability of 0.984.</p><h3>Graphical Abstract</h3>\u0000<div><figure><div><div><picture><source><img></source></picture></div></div></figure></div></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-08-07","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145163065","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Isolation and Screening of Indigenous Microalgal Strains from Diverse Ecological Niches for Lipid and Pigment Production Relevant to Sustainable Biorefinery Applications","authors":"Aravind K. Vijay,&nbsp;Syama Prabha,&nbsp;Said Ali M. Salim,&nbsp;Atheesh O. S.,&nbsp;Basil George","doi":"10.1007/s12155-025-10869-1","DOIUrl":"10.1007/s12155-025-10869-1","url":null,"abstract":"<div><p>Exploring microalgae diversity has been a longstanding endeavour for producing valuable metabolites with significant biotechnological applications. This study investigates the microalgal diversity present in three distinct agroecological zones of Kerala, each varying considerably in topography, rainfall patterns, and altitude. A total of 209 microalgae strains were identified from 19 selected sites, showcasing the richness of diversity across these regions. Furthermore, 30 microalgae strains belonging to chlorophyceae (16) and cyanophyceae (14) were isolated and screened for their lipid and pigment productivity potential. Among the Chlorophyceae, <i>Coelastrella</i> sp. exhibited the highest growth rate (0.096 µ/day), while <i>Ankistrodesmus falcatus</i> and <i>Monoraphidium contortum</i> demonstrated the highest lipid productivity (23.69–22.17 mg/L/day). Carotenoid productivity was notably higher in <i>Chlorella</i> spp. and <i>Monoraphidium contortum</i> (343–268 µg/L/day). Among the Cyanophyceae members, <i>Arthrospira platensis</i> showed the highest growth rate (0.110 µ/day), followed by <i>Oscillatoria</i> sp. (0.89–0.78 µ/day), both exhibiting significant phycocyanin productivity (11.7–2.9 mg/L/day). The phycoerythrin productivity was exceptionally high in <i>Synechococcus</i> sp. and <i>Leptolyngbya</i> sp. (3.4–1.7 mg/L/day). Additionally, <i>Synechocystis</i> sp. exhibited moderate carotenoid content (4.5–3.8 µg/mg), while heterocystous <i>Nostoc</i> sp. and <i>Anabaena</i> sp. showed promising protein content (30–35%). The study highlights the potential of these isolated strains as promising candidates for biofuel and pigment production and their integration into a microalgae-based biorefinery.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-28","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145171078","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Sugarcane Bagasse Polysaccharides Decomposition by the Cockroach Digestive System","authors":"D. Pagliuso,&nbsp;A. Grandis,&nbsp;D. G. Santos,&nbsp;C. Ferreira,&nbsp;W. R. Terra,&nbsp;C. Cardoso,&nbsp;A. C. Pimentel,&nbsp;F. J. Fuzita,&nbsp;J. Pereira,&nbsp;I. Ramos,&nbsp;L. Quezia,&nbsp;A. C. Bahia,&nbsp;N. Heise,&nbsp;M. S. Buckeridge,&nbsp;E. A. Machado","doi":"10.1007/s12155-025-10872-6","DOIUrl":"10.1007/s12155-025-10872-6","url":null,"abstract":"<div><p>Cockroaches are omnivorous insects that consume a diverse diet, including lignocellulose. This study investigated <i>Periplaneta americana, which was</i> fed exclusively on sugarcane bagasse, to evaluate biomass degradation across gut chambers and provide helpful information to the bioenergy industry. We analyzed enzyme activity, the composition of monosaccharides from bagasse, and lignin content in gut sections, feces, and food bolus. The fiber's morphology was also evaluated. Bagasse breakdown occurs sequentially (foregut, midgut, hindgut) through mechanical grinding and glycosyl hydrolase activity. The foregut primarily reduces particle size (&gt; 90%) through chewing and enzymatic action, aided by chitinous teeth. Sugarcane bagasse in the midgut resembled the foregut's morphology, while the hindgut and feces displayed microorganisms and small surface holes. The study also explored links between the distribution of monosaccharides and glycosyl hydrolase activity in gut chambers. Monosaccharide profiling revealed elevated levels of rhamnose (~ 1. 1.2 μg·mg⁻¹ CW) and galactose (~ 5 μg·mg⁻¹ CW) in the foregut/midgut, with xylose peaking in the foregut (~ 15 μg·mg⁻¹ CW). Fucose, mannose, arabinose, non-cellulosic, and cellulosic (~ 1, 4, 4.2, 6, 6.2, 7, and 48 μg· mg¹ CW, respectively) remained stable across gut regions. Lignin (20% of the cell wall) persisted undigested in bagasse and feces (~ 20% dry weight). Enzymatic profiling of digestive enzymes showed that the foregut and midgut exhibited higher soluble enzymatic activities against βGlc (~ 3), βXyl (~ 1. 8), αXyl (~ 0. 02), βGal (~ 1.6. 6), αGal (~ 1), βMan (~ 1.3. 3), αAra (~ 0. 0.8), βClb (~ 0. 8), βGlca (~ 0.3. 3), and αRha (~ 0. 006 μmol sugar min⁻¹ mg ptn⁻¹). The hindgut (the main microbial chamber) showed lower activity for most enzymes. Fecal analysis indicated digestion/absorption of ~ 50% cellulosic glucose, 25% non-cellulosic glucose, 21% arabinose, and 26% xylose. These findings suggest that <i>P. americana</i> is a promising hemicellulose/cellulose degradation model without lignin breakdown under mesophilic conditions.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169809","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Advancements in Heterogeneous Biobased and Bio-Composite Catalysts: A Comprehensive Review on Synthesis, Characterizations, and Applications in Biodiesel Production","authors":"Lata Deso Abo,&nbsp;Hirpha Adugna Areti","doi":"10.1007/s12155-025-10868-2","DOIUrl":"10.1007/s12155-025-10868-2","url":null,"abstract":"<div><p>The synthesis of biodiesel from inedible feedstocks using bio-composite heterogeneous catalysts has emerged as a sustainable strategy to address global warming, enhance energy security, and preserve biodiversity. This review highlights advancements in eco-friendly catalysts derived from waste biomass, including eggshells, banana peels, agricultural residues (such as cocoa pod husks and sugarcane bagasse), and industrial by-products. These catalysts, which include CaO-based composites (such as Zn-doped CaO and Zr/CaO), sulfonated carbon materials, silica-impregnated CaO, and metal oxide-supported systems (such as ZrO₂/bamboo leaf ash and Cu/TiO₂), are designed to improve catalytic efficiency, reusability, and sustainability. Characterization techniques such as FTIR, XRD, SEM, XPS, and TGA confirm their structural stability, active sites, and thermal resilience, all of which are critical for optimizing biodiesel yield. The use of waste-derived catalysts not only reduces reliance on edible feedstocks but also promotes circular economy principles by valorizing biowaste. This review discusses challenges related to scalability, long-term stability, and cost-effective synthesis, as well as opportunities to refine catalyst design through advanced functionalization and hybrid systems. By merging sustainable chemistry with industrial applications, bio-composite catalysts offer a pathway to decarbonize energy systems, support local economies in developing regions, and align with global sustainability goals. The review emphasizes the need for interdisciplinary innovation to unlock the full potential of waste-derived catalysts in achieving a low-carbon future.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-25","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145169254","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Effect of Primary Air on Performance of a Natural Draft Dual-Purpose Gasifier Stove: An Experimental Study","authors":"Himanshu,&nbsp;Deepanshu Awasthi,&nbsp;Kunwar Pal","doi":"10.1007/s12155-025-10870-8","DOIUrl":"10.1007/s12155-025-10870-8","url":null,"abstract":"<div><p>Biochar production and household energy utilization have garnered considerable attention in developing nations worldwide for over a decade. Gasification-based cookstoves mitigate numerous issues related to conventional cooking methods while effectively producing biochar. The novelty of this work lies in utilizing the gasifier stove for biochar production while simultaneously offering a clean cooking solution that empowers the livelihood of the rural population. The present study is carried out to investigate the effects of primary airflow on the thermal performance and biochar yield of a natural draft gasification technology-based improved cookstove. The inlet area of primary air varies from 25 to 100% in increments of 25%, producing biochar samples BC 25, BC 50, BC 75, and BC 100. The produced biochar is further characterized by performing proximate and ultimate analysis, X-ray diffraction analysis, FTIR analysis, and BET surface area analysis. The developed cookstove exhibits thermal efficiency in the range of 26.96–28.52%, while the biochar yield varies between 15.2 and 20.4%. The emission of carbon monoxide calculated per unit useful energy delivered to the pot ranges from 2.48 to 3.89 g/MJ_D corresponding to various airflow rates of primary air. The calorific value and percentage carbon content in the produced biochar samples range between 7424 and 8329 kcal/kg and 84.42 and 86.82%, respectively. The surface area of the biochar sample is increased three times with the increment in the primary air inlet area from 25 to 100%.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-19","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145167722","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}

{"title":"Research on Volatile Fatty Acids Production During the Anaerobic Digestion of Corn Stover Waste in Northeast China: Freeze-Thaw Pretreatment and Effect of Initial pH","authors":"Nan Qi,&nbsp;Yinuo Zhao,&nbsp;Chaoxing Zhang,&nbsp;Ying Xu,&nbsp;Zixi Wei,&nbsp;Hongxu Bao,&nbsp;Jian Wang","doi":"10.1007/s12155-025-10866-4","DOIUrl":"10.1007/s12155-025-10866-4","url":null,"abstract":"<div><p>The low temperatures in Northeastern China offer a promising and energy-efficient approach for lignocellulose pretreatment. The effects of freeze-thaw pretreatment on the microstructure of corn stover hydrolysis characteristics and fermentation acid production were investigated using corn stover as the raw material. The experimental results demonstrated that the reducing sugar release and soluble chemical oxygen demand (SCOD) values of corn stover following freeze-thaw pretreatment exhibited increases of 15.8% to 67.0% and 13.9% to 68.9%, respectively, compared to those of the control group. Freeze-thaw pretreatment modifies the microstructure of corn stover by breaking hydrogen bonds in the amorphous region between cellulose and hemicellulose, facilitating lignin removal. The acid yield of the treated corn stover under optimal pretreatment conditions is enhanced by up to 77.9% compared with the control group. The initial pH pronouncedly influenced the acid yield of anaerobic fermentation of corn stover, with the highest acid yield of 3.8 g/L observed at pH values between 7.5 and 8. This study provides theoretical guidance for the industrial development of a low-cost and low-energy consumption pretreatment method in lignocellulose wastes.</p></div>","PeriodicalId":487,"journal":{"name":"BioEnergy Research","volume":"18 1","pages":""},"PeriodicalIF":3.0,"publicationDate":"2025-07-14","publicationTypes":"Journal Article","fieldsOfStudy":null,"isOpenAccess":false,"openAccessPdf":"","citationCount":null,"resultStr":null,"platform":"Semanticscholar","paperid":"145165609","PeriodicalName":null,"FirstCategoryId":null,"ListUrlMain":null,"RegionNum":3,"RegionCategory":"工程技术","ArticlePicture":[],"TitleCN":null,"AbstractTextCN":null,"PMCID":"","EPubDate":null,"PubModel":null,"JCR":null,"JCRName":null,"Score":null,"Total":0}