以马铃薯皮水解物为碳源的极性微藻衣藻 RCC2488 (Malina) 的混养培养

IF 3.1 3区 工程技术 Q3 ENERGY & FUELS
Shah Rucksana Akhter Urme, Laura Barth, Leobardo Serrano-Carreón, Alfredo Martinez, Daniela Morales-Sánchez
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引用次数: 0

摘要

极地微藻Chlamydomonas sp. RCC2488 (malina)在光养条件下表现出较高的PUFA产量,但没有异养或混合营养生长的证据。因此,在异养和混合营养条件下评估了生长和跨膜碳(葡萄糖)运输。在与葡萄糖混合营养的条件下,微藻以0.015 μmol/g/min的速率运输碳,获得0.4 g/L的生物量,PUFA含量高达122.5 mg/g。未观察到异养生长和转运。对马铃薯皮废料等替代碳源的利用进行了评价。采用1%硫酸,121℃,120 min预处理,α-淀粉酶和淀粉葡糖苷酶水解。得到的水解产物(PPH1和PPH3)释放高达46.3 g/L的葡萄糖,还原糖转化率为46%。但这些水解产物也分别含有高达4.7和8.5 g/L的糠醛和羟甲基糠醛(HMF),使微藻生长降低至0.07 g/L,促进了TAG的积累74.7 g/g。水洗额外步骤(PPH2)去除这些有毒化合物,使生物质和PUFA浓度与纯葡萄糖相似。因此,利用马铃薯皮作为微藻培养的高效碳源,必须进行预处理和水解优化,包括去除有毒副产物。衣藻RCC2488 (malina)的混合营养培养是可能的,但不是理想的,因为在光养条件下可以获得更高的生长。
本文章由计算机程序翻译,如有差异,请以英文原文为准。
Mixotrophic Cultivation of Polar Microalga Chlamydomonas sp. RCC2488 (Malina) Using Potato Peel Hydrolysates as Carbon Source

The polar microalga Chlamydomonas sp. RCC2488 (malina) exhibits high PUFA production under phototrophic conditions, but there is no evidence of heterotrophic or mixotrophic growth. Hence, the grow and transmembrane carbon (glucose) transport was assessed under heterotrophic and mixotrophic conditions. The microalga transported carbon at a rate of 0.015 μmol/g/min, achieving 0.4 g/L of biomass with high PUFA content (122.5 mg/g) under mixotrophic conditions with glucose. No heterotrophic growth and transport were observed. The use of an alternative carbon source such as potato peel waste was evaluated. This material was subjected to an acid-hydrothermal pretreatment with 1% sulfuric acid at 121 °C for 120 min followed by enzymatic hydrolysis with α-amylase and amyloglucosidase. The resulting hydrolysates (PPH1 and PPH3) released up to 46.3 g/L of glucose with a reducing sugar conversion of 46%. But these hydrolysates also presented up to 4.7 and 8.5 g/L of furfural and hydroxymethylfurfural (HMF), respectively, which decreased the microalgal growth to 0.07 g/L and promoted the accumulation of 74.7 g/g of TAG. A water-washing extra step (PPH2) removed these toxic compounds achieving biomass and PUFA concentrations similar to the ones obtained with pure glucose. Therefore, pretreatment and hydrolysis optimization including toxic by-product removal are necessary to use potato peel as an efficient carbon source for microalgae cultivation. Mixotrophic cultivation of Chlamydomonas sp. RCC2488 (malina) was possible but not ideal since higher growth is attained under phototrophic conditions.

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来源期刊
BioEnergy Research
BioEnergy Research ENERGY & FUELS-ENVIRONMENTAL SCIENCES
CiteScore
6.70
自引率
8.30%
发文量
174
审稿时长
3 months
期刊介绍: BioEnergy Research fills a void in the rapidly growing area of feedstock biology research related to biomass, biofuels, and bioenergy. The journal publishes a wide range of articles, including peer-reviewed scientific research, reviews, perspectives and commentary, industry news, and government policy updates. Its coverage brings together a uniquely broad combination of disciplines with a common focus on feedstock biology and science, related to biomass, biofeedstock, and bioenergy production.
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